CN110051602B

CN110051602B - Skin moistening, moisturizing and repairing emulsion and preparation method thereof

Info

Publication number: CN110051602B
Application number: CN201910398033.9A
Authority: CN
Inventors: 丁一兵; 康立峰; 吴荣荣
Original assignee: Oriental Aibao Beijing Maternal And Child Health Technology Co ltd
Current assignee: Oriental Aibao Beijing Maternal And Child Health Technology Co ltd
Priority date: 2019-05-14
Filing date: 2019-05-14
Publication date: 2021-11-26
Anticipated expiration: 2039-05-14
Also published as: CN110051602A

Abstract

The invention discloses a skin moistening and moisturizing repair emulsion which comprises the following components in percentage by mass: 0.5 to 2 percent of sodium hyaluronate, 1 to 3 percent of almond extract, 0.5 to 5 percent of zostera marina extract, 2 to 5 percent of Japanese seaweed extract, 1.2 to 4 percent of chicory extract, 0.5 to 3 percent of parsley extract, 1 to 3 percent of broccoli extract, 2 to 3 percent of oat extract, 1 to 4 percent of arginine, 2 to 6 percent of polyalcohol, 0.2 to 0.5 percent of preservative, 0.02 to 0.06 percent of thickening agent and the balance of water; heating and mixing Japanese seaweed extract, chicory extract, parsley extract, broccoli extract and water, adding a thickening agent in a heat preservation state, uniformly mixing, and then adding mixed solution of sodium hyaluronate, polyalcohol, water, almond extract, zostera marina extract and oat extract, arginine, a preservative and an acid-base regulator to prepare the emulsion. The raw materials of the invention are safe and harmless, and can obviously improve the skin state and improve the skin health degree; the preparation method is easy for industrial production.

Description

Skin moistening, moisturizing and repairing emulsion and preparation method thereof

Technical Field

The invention belongs to the technical field of skin care products, and particularly relates to skin moistening, moisturizing and repairing emulsion and a preparation method thereof.

Background

Nowadays, with the aggravation of environmental pollution, the increase of working pressure of people, irregular work and rest and other factors, the problems of skin disorders such as unbalanced water and oil and acne growing are puzzling people day by day, and moisturizing cosmetics are receiving more and more attention of consumers; moisturizing cosmetics are produced by moisturizing agents to maintain the moisture content of the stratum corneum, maintain skin elasticity, and promote the repair of skin barrier function.

Although most of the traditional cosmetic moisturizing agents have good moisturizing effect and stability, the traditional cosmetic moisturizing agents generally have sticky feeling, the skin feeling after use is not fresh enough, the moisturizing time is not long enough, and even some moisturizing agents can absorb moisture in the inner layer of the skin under certain conditions, so that the skin is drier; or the closure is too strong, which causes unsmooth skin excretion and certain obstruction to the metabolism function of the skin.

Chinese patent application No. CN201811553518.2 discloses a water-moistening, brightening and moisturizing crystal lotion, which comprises the following components calculated by mass concentration: 0.6% of seaweed extract, 0.6% of cod liver oil, 0.5% of turtle oil, 0.135% of coral powder, 0.385% of jellyfish extract, 0.5% of sea fennel extract, 0.6% of chlorella pyrenoidosa extract, 3% of robinia pseudoacacia extract, 2% of beeswax, 4.55% of coconut oil, 3.5% of glycerin, 5.0% of soybean egg scale fat, 2.0% of sandalwood essential oil, 3.0% of prunus almond oil, 2.0% of honey, 1.0% of olive oil and 0.6% of wheat oil; the moisturizing crystal prepared by using natural materials focuses on improvement of moisture content, strength, viscoelasticity and the like of skin, but the problems of poor resistance, acne, roughness, melanin precipitation, wrinkles and the like of the skin still occur when the moisture content of the skin is only improved without paying attention to water-oil balance of the skin.

Therefore, there is a need to develop a natural skin care product to improve the skin condition more comprehensively.

Disclosure of Invention

The invention aims to provide a skin moistening and moisturizing repair emulsion aiming at the problems in the prior art, the pure natural plant extract is adopted, the effects of very high water replenishing and moisturizing, skin cell regeneration promotion, skin cell resistance improvement and the like can be achieved, in addition, the plant extract has small side effect, small irritation to skin, rich sources, certain plants even being edible and high in safety;

the invention also provides a preparation method of the skin moisturizing and repairing emulsion, which has simple preparation process, does not need complex and large-scale equipment, is suitable for large-scale production in factories and is also suitable for manual DIY.

In order to achieve the purpose, the invention adopts the technical scheme that:

the skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 0.5 to 2 percent of sodium hyaluronate, 1 to 3 percent of almond extract, 0.5 to 5 percent of zostera marina extract, 2 to 5 percent of Japanese seaweed extract, 1.2 to 4 percent of chicory extract, 0.5 to 3 percent of parsley extract, 1 to 3 percent of broccoli extract, 2 to 3 percent of oat extract, 1 to 4 percent of arginine, 2 to 6 percent of polyalcohol, 0.2 to 0.5 percent of preservative, 0.02 to 0.06 percent of thickening agent and the balance of water.

The chicory extract, the parsley extract and the broccoli extract are compounded and synergized to regulate the water-oil balance of skin, wherein the chicory is rich in tannin, tannic acid, dietary fiber, carotene, vitamin B2, vitamin C, potassium and iron, and has good moisturizing and antioxidant effects; celery is rich in tannin, beta-carotene, polyacetylene and luteolin, and has good oxidation resistance and anti-irritation effects; the broccoli is rich in tannin, flavonoid compounds, beta-carotene, mineral substances, vitamin A, vitamin C, vitamin K, potassium, manganese and the like, can promote the healing of damaged skin, and also has strong oxidation resistance and aging delaying; the chicory extract, the parsley extract and the broccoli extract form a water-oil balance regulating factor, firstly, lipid synthesis is promoted, the water-oil balance of skin is kept, secondly, hyaluronic acid synthesis is promoted, deep moisture preservation is achieved, and thirdly, the chicory extract is rich in the powerful antioxidant factor tannin, can effectively remove free radicals in vivo, protects skin tissues from inflammation and damage of harmful factors related to cancer, can reduce inflammation generation by inhibiting sebum oxidation, can regulate the balance of water and sebum in skin, strengthens skin tissues, and enables the skin to be fresh, smooth, glossy and elastic. 5 alpha-reductase promotes the generation of Dihydrotestosterone (DHT), which is a male hormone and can stimulate sebaceous gland secretion, cause pore blockage and be not beneficial to nutrient absorption; the seaweed extract can inhibit 5 alpha-reductase activity, thereby inhibiting sebum accumulation; in addition, tannin in the chicory extract, the parsley extract and the broccoli extract can contract and expand pores and avoid the pores from being large, so that the skin pores can be enabled to be fresh and clean, the nutrient substance absorption can be facilitated, acne caused by excessive sebum secretion can be avoided, and the skin is enabled to be glossy and smooth. The Zostera marina extract and maltose are polysaccharide materials, and can produce effects of tranquilizing, relieving and resisting inflammation when applied to skin. The fatty oil contained in the almond extract can soften the horny layer of the skin, soften skin cells, increase the absorption capacity of the skin, form a hydrophobic film on the surface of the skin, inhibit the evaporation of water in the epidermis, prevent the skin from being dry and rough and provide a protective effect; in addition, the almond extract is cooperated with the sodium hyaluronate moisturizing ingredient, so that the almond extract can obviously play a role in removing yellow in a skin care product; the semen Armeniacae amarum extract also contains components capable of inhibiting in vivo active tyrosinase, and can help eliminate facial pigmentation, freckle, dark spot, etc., to achieve whitening effect. The oat extract contains beta-glucan which acts on cellular molecular level, but not acts on the surface of the skin, and is a true active matter for internally changing the skin condition; it has film-forming properties, and helps to keep the skin moist; oxidation resistance, obvious reduction of skin wrinkles and improvement of skin elasticity; protecting skin from being damaged by ultraviolet rays, and improving the tolerance of the skin against external stimulation; the oat extract is added into the emulsion, so that the transdermal absorption rate of other active ingredients in the emulsion can be increased, and the efficacy of other ingredients can be enhanced to a certain extent. Arginine can improve the skin immunologic function, regulate water, acid and alkali, balance grease, improve the anti-allergy capability of sensitive skin and prevent wrinkles; can also activate the activity of superoxide dismutase peroxide of skin cells, dispel excessive free radicals of skin cells and effectively delay skin aging; it also has effects in activating phagocytic function of macrophage, enhancing toxin expelling and removing functions of lymphatic system, effectively blocking the invasion of external harmful substances to skin, and decomposing and excreting harmful substances and aged cells in vivo. In addition to the synergistic effect of the sodium hyaluronate and the almond extract, the sodium hyaluronate can permeate into the epidermis layer of the skin to promote the supply of skin nutrition and the excretion of waste, thereby preventing the skin from aging and playing the roles of maintaining beauty and keeping young; sodium hyaluronate can also promote the regeneration of skin at the injured part by promoting the proliferation and differentiation of epidermal cells and scavenging oxygen free radicals; the sodium hyaluronate is used in skin care products, has good lubrication feeling and good skin protection.

Preferably, the components are specifically: 0.8 to 1.6 percent of sodium hyaluronate, 1.2 to 2.6 percent of almond extract, 0.8 to 4.2 percent of zostera marina extract, 2.3 to 4.5 percent of Japanese seaweed extract, 1.5 to 3.2 percent of chicory extract, 1 to 2.5 percent of parsley extract, 1.3 to 2.6 percent of broccoli extract, 2.1 to 2.8 percent of oat extract, 1.4 to 3.2 percent of arginine, 2.6 to 5.2 percent of polyalcohol, 0.2 to 0.5 percent of preservative, 0.02 to 0.06 percent of thickening agent and the balance of water.

More preferably, the components are in particular: 1.2% sodium hyaluronate, 1.8% almond extract, 2.4% zostera marina extract, 3% japanese seaweed extract, 2.1% chicory extract, 1.7% parsley extract, 1.8% broccoli extract, 2.5% oat extract, 2% arginine, 3.5% polyol, 0.3% preservative, 0.04% thickener, and balance water.

Preferably, the polyalcohol is formed by combining glycerol and butanediol in a mass ratio of 1: 1-3. Butanediol is a micromolecular moisturizing component and also has a certain bacteriostatic action; the glycerin can absorb moisture in the air to moisturize the skin, can lock the moisture in the skin and prevent the moisture from losing, and has good moisturizing and moistening effects.

Preferably, the preservative is formed by combining octyl glycol and ethyl hexyl glycerol in a mass ratio of 1.2-1.5: 1. The addition of ethyl hexyl glycerol can reduce the addition amount of octyl glycol, and promote the antibacterial effect of fatty acids and lipids in the extract.

Preferably, the thickening agent is formed by combining xanthan gum and hydroxyethyl cellulose according to the mass ratio of 0.5-1.5: 1. Xanthan gum has a special macromolecular structure and colloidal characteristics, and when used in an emulsion, not only can improve the viscosity of the emulsion, but also can be used as an emulsifier.

A preparation method of a skin moistening and moisturizing repair emulsion comprises the following steps:

s1, weighing the raw materials according to mass percentage;

s2, dissolving sodium hyaluronate and polyalcohol into 20% water by mass, stirring and heating to 85-90 ℃, simultaneously dropwise adding an almond extract, a zostera marina extract and an oat extract, and then cooling to 60-70 ℃ to prepare a mixed solution A;

s3, adding the Japanese seaweed extract, the chicory extract, the parsley extract and the broccoli extract into the balance of 80 mass percent of water, stirring and heating to 60-70 ℃, adding the thickening agent while stirring in a heat preservation state, and preparing a mixed solution B;

s4, simultaneously dripping the mixed solution A and arginine into the mixed solution B, uniformly mixing, dripping a preservative, and adding an acid-base regulator to adjust the pH value of the mixed solution to 6.5-7.0; cooling to room temperature to obtain the emulsion.

Step S2, adding the almond extract, the zostera marina extract and the oat extract into a hot mixed solution of sodium hyaluronate, polyhydric alcohol and water, so as to increase the solubility of active ingredients in the almond extract, the zostera marina extract and the oat extract; step S3, mixing Japanese sea grass extract, chicory extract, parsley extract, broccoli extract and water to obtain a mixed solution, adding a thickening agent, and reducing the mobility of active molecules by the form of the thickening agent dissolved in water to keep the active molecules in a uniform dispersion state; and then, in step S4, the mixed solution a is added dropwise to the mixed solution B, so that the active ingredients in the mixed solution a slowly enter the mixed system, and the active ingredients are uniformly dispersed into the mixed system under the migration action of water molecules in the mixed solution a, so as to ensure that each active ingredient of the prepared emulsion exists uniformly and stably.

Compared with the prior art, the invention has the beneficial effects that:

1. the combination of the almond extract, the zostera marina extract, the Japanese seaweed extract, the chicory extract, the parsley extract, the broccoli extract and the oat extract is adopted to achieve the purposes of regulating the water-oil balance of the skin, improving the moisture retention capacity of the skin, preventing acne on the skin, improving the oxidation resistance of the skin, improving the absorption and permeation of the skin to active ingredients and avoiding the mud rubbing phenomenon;

2. the preparation method of the emulsion provided by the invention has the advantages that the raw materials are simple and easy to obtain, most of the raw materials are extracted from pure natural plants, the health is realized, the safety is high, and the cost is low; the preparation method is easy for industrial production.

Drawings

FIG. 1 is a graph showing the results of the test for antibacterial activity (against Staphylococcus aureus) of the present invention.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 1.2% sodium hyaluronate, 1.8% almond extract, 2.4% zostera marina extract, 3% japanese seaweed extract, 2.1% chicory extract, 1.7% parsley extract, 1.8% broccoli extract, 2.5% oat extract, 2% arginine, 3.5% polyol, 0.3% preservative, 0.04% thickener, water to 100%.

Wherein the polyalcohol is formed by combining glycerol and butanediol according to the mass ratio of 1: 2; the preservative is formed by combining caprylyl glycol and ethyl hexyl glycerol according to the mass ratio of 1.3: 1; the thickening agent is formed by combining xanthan gum and hydroxyethyl cellulose according to the mass ratio of 1: 1.

s1, weighing the raw materials according to mass percentage;

s2, dissolving sodium hyaluronate and polyalcohol in 20% by mass of water, stirring and heating to 88 ℃, simultaneously dropwise adding an almond extract, a zostera marina extract and an oat extract, and then cooling to 65 ℃ to prepare a mixed solution A;

s3, adding the Japanese seaweed extract, the chicory extract, the parsley extract and the broccoli extract into the balance of 80 mass percent of water, stirring and heating to 65 ℃, adding the thickening agent while stirring in a heat preservation state, and preparing a mixed solution B;

s4, simultaneously dripping the mixed solution A and arginine into the mixed solution B, uniformly mixing, dripping a preservative, and adding an acid-base regulator to adjust the pH value of the mixed solution to 6.8; cooling to room temperature to obtain the emulsion.

Example 2

This embodiment is substantially the same as embodiment 1 except that: the skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 0.5% sodium hyaluronate, 1% almond extract, 5% zostera marina extract, 2% japanese seaweed extract, 4% chicory extract, 2.8% parsley extract, 1.2% broccoli extract, 2% oat extract, 1.3% arginine, 4.7% polyol, 0.5% preservative, 0.02% thickener, water to 100%.

Example 3

This embodiment is substantially the same as embodiment 1 except that: the skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 2% sodium hyaluronate, 3% almond extract, 0.6% zostera marina extract, 4.7% japanese seaweed extract, 1.3% chicory extract, 0.9% parsley extract, 2.8% broccoli extract, 2.9% oat extract, 3.2% arginine, 2.2% polyol, 0.2% preservative, 0.06% thickener, water to 100%.

Example 4

This embodiment is substantially the same as embodiment 1 except that: the skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 0.8% sodium hyaluronate, 1.2% almond extract, 4.2% zostera marina extract, 2.3% japanese seaweed extract, 1.5% chicory extract, 1.2% parsley extract, 1.5% broccoli extract, 2.5% oat extract, 1.6% arginine, 2.8% polyol, 0.3% preservative, 0.03% thickener, water added to 100%.

Example 5

This embodiment is substantially the same as embodiment 1 except that: the skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 1.6% sodium hyaluronate, 2.6% almond extract, 0.8% zostera marina extract, 4.5% japanese seaweed extract, 3.2% chicory extract, 2.3% parsley extract, 2.5% broccoli extract, 2.6% oat extract, 3% arginine, 5% polyol, 0.4% preservative, 0.05% thickener, water to 100%.

Example 6

This embodiment is substantially the same as embodiment 1 except that: the polyalcohol is composed of glycerol and butanediol at a mass ratio of 1: 1.

Example 7

This embodiment is substantially the same as embodiment 1 except that: a preparation method of a skin moistening and moisturizing repair emulsion comprises the following steps:

s1, weighing the raw materials according to mass percentage;

s2, dissolving sodium hyaluronate and polyalcohol in 20% by mass of water, stirring and heating to 85 ℃, simultaneously dropwise adding an almond extract, a zostera marina extract and an oat extract, and then cooling to 62 ℃ to prepare a mixed solution A;

s3, adding the Japanese seaweed extract, the chicory extract, the parsley extract and the broccoli extract into the balance of 80 mass percent of water, stirring and heating to 68 ℃, adding the thickening agent while stirring in a heat preservation state, and preparing a mixed solution B;

s4, simultaneously dripping the mixed solution A and arginine into the mixed solution B, uniformly mixing, dripping a preservative, and adding an acid-base regulator to adjust the pH value of the mixed solution to 6.5; cooling to room temperature to obtain the emulsion.

Comparative example 1

This embodiment is substantially the same as embodiment 1 except that: the skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 2.5% sodium hyaluronate, 0.5% almond extract, 2.4% zostera marina extract, 3% japanese seaweed extract, 2.1% chicory extract, 1.7% parsley extract, 1.8% broccoli extract, 2.5% oat extract, 2% arginine, 3.5% polyol, 0.3% preservative, 0.04% thickener, water to 100%.

Comparative example 2

This embodiment is substantially the same as embodiment 1 except that: it does not contain Japanese seaweed extract.

Comparative example 3

This embodiment is substantially the same as embodiment 1 except that: does not contain broccoli extract.

Comparative example 4

This embodiment is substantially the same as embodiment 1 except that: the skin moistening and moisturizing repair emulsion comprises the following components in percentage by mass: 1.2% sodium hyaluronate, 1.8% almond extract, 2.4% zostera marina extract, 3% japanese seaweed extract, 1.0% chicory extract, 3.2% parsley extract, 1.5% broccoli extract, 2.5% oat extract, 2% arginine, 3.5% polyol, 0.3% preservative, 0.04% thickener, water to 100%.

Comparative example 5

This embodiment is substantially the same as embodiment 1 except that: the polyol is glycerol.

Comparative example 6

s1, weighing the raw materials according to mass percentage;

s2, mixing sodium hyaluronate, polyalcohol and water, stirring and heating to 88 ℃, sequentially adding an almond extract, a zostera marina extract, a Japanese seaweed extract, a chicory extract, a parsley extract, a broccoli extract, an oat extract and arginine, uniformly stirring, cooling to 65 ℃, adding a preservative and a thickening agent, continuously stirring uniformly, and adding an acid-base regulator to adjust the pH value of the mixed solution to 6.8; cooling to room temperature to obtain the emulsion.

Test example 1 skin feel evaluation

Skin feel evaluation was performed on the emulsions prepared in examples 1 to 12 and comparative examples 1, 5 and 6, and the skin feel evaluation method was mainly to examine the spreadability (wet feel, spreadability and absorbency) and the after-application feel (gloss and stickiness), as shown in table 1 below:

TABLE 1 skin feel evaluation index

The evaluation index range is 0-10 points. Selecting 50 volunteers of 18-38 years old, wherein 40 female volunteers are selected, and 10 male volunteers are selected; the volunteers' forearms were free of cream, perfume, alcohol wiped and fingertips cleaned; sampling 0.2g was done in 4.5cm diameter staggered circles (clockwise, approximately 2 seconds/time, up to 20 times) on the forearm, one index finger and the other finger felt the skin after application. The samples were scored against the criteria described in table 1 and, for final statistical comparison, the results of the tests are shown in table 2 below:

table 2 skin feel evaluation results

As can be seen from the test results in table 2 above:

1) from the test results of examples 1 to 5 and comparative example 1, it can be seen that: the emulsion obtained by combining the raw materials according to the weight percentage has the integral use effect, particularly the absorbability superior to that of the emulsion obtained by combining other weight percentages; this is mainly because in the emulsion system, the various raw materials cooperate with each other to exert the overall use effect, and only within a proper proportion range, the efficacy can be exerted to the best. In addition, the absorption of the emulsion by the human body increases with the increase of the active ingredient, but the absorption does not change much when a certain critical point is reached, which is mainly related to the saturation of the absorption of the active ingredient by the human skin.

2) From the test results in example 6 and comparative example 5, it can be seen that: the combination of two polyols is adopted, and two moisturizing components with different molecular grades act on different layers of skin moisturizing, so that the prepared emulsion has certain difference in usability.

3) From the test results in example 7 and comparative example 6, it can be seen that: the emulsion prepared by adopting different methods and process parameters has reduced wetting feeling, spreadability and absorbability after being coated, and the overall glossiness and viscosity of the coated emulsion are not as good as those of the emulsion prepared in the example 1.

Test example 2 skin irritation test

1) Acute skin irritation test in animals

The evaluation is carried out according to the method of national cosmetic safety evaluation national standard GB-7919-87, and the tested animal is a rabbit. Taking 0.1g of sample, uniformly coating the sample on the skin of one side of a spine of a rabbit with a unhaired area of 2.5cm multiplied by 2.5cm, covering the skin with a layer of oiled paper, fixing the oiled paper and a bandage with a non-irritant adhesive plaster for 24 hours, washing away the residual sample with warm water after 24 hours, observing the reaction of the skin within 24 hours, and integrating two phenomena of erythema and edema, and judging according to the scoring standard in the table 3.

TABLE 3 skin irritation score criteria

Among them, stimulation intensity evaluation (total score of 8): the integral is less than or equal to 0.4, and is nonirritating; integration 0.5-1.9 is mild irritability; the integral of 2.0-5.9 is moderate irritation; an integral of 6.0 or more is strongly irritating.

The emulsion samples prepared in examples 1-12 were tested, and the integral was not more than 0.4, and the samples were non-irritating.

2) Human skin irritation test

On the basis of animal stimulation tests, the emulsion samples prepared in examples 1-12 were selected for testing. A total of 50 volunteers aged 18-38 were selected, 40 female and 10 male. Taking 0.25g of sample in a medical skin test patch, sticking the sample on the inner side of the upper arm cleaned by the volunteer, taking the skin test patch after lasting for 24 hours, and judging according to the scoring standard in the table 3.

The measurement results show that: the tests of the emulsion samples prepared in the embodiments 1 to 7 show that the emulsion prepared by the invention has no irritation, and the emulsion prepared by the invention has no obvious irritation and is safe to use when being used for a human body.

Test example 3 antibacterial Properties

During the use of cosmetics, the most commonly exposed microorganisms are mostly molds of the bacterial and fungal type. When the antibacterial property of the emulsion sample is tested, the test comprises respectively inoculating Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli on nutrient agar inclined plate placed in dark cabinet at room temperature, culturing at 35 deg.C for 48 hr, washing with sterile physiological saline containing 0.05% polysorbate-80 to make the final cell concentration be 10⁸one/mL. In addition, Candida albicans was inoculated on a Sasa grape agar slant plate placed in a dark cabinet at room temperature, and cultured at 20 ℃ for 48h, and the rest operations were identical to the above.

Respectively taking 0.2mL of the 4 bacterial liquids, adding the 0.1mL of the emulsions into a blank emulsion with 20mL, respectively taking 0,1,2,3,4,5,8,24 hours and 7,14,21 and 28 days by taking physiological saline as a control, adding 20mL of sterilized agar at 55 ℃, gently mixing the liquids before solidification, culturing the liquids at 35 ℃ for 3 days (culturing the candida albicans at 20 ℃ for 5 days), and recording the colony count.

The emulsion prepared in example 1 is selected for test, the blank emulsion kills pseudomonas aeruginosa, staphylococcus aureus, escherichia coli and candida albicans within 24h by 100 percent, and the regeneration of the bacteria is not seen within 28 days. The test results of staphylococcus aureus are shown in fig. 1:

in figure 1 it is shown that staphylococcus aureus was added to the blank emulsion at 112/mL and rapidly reduced to zero after the 8h trial. This demonstrates that the emulsions prepared in the examples have good bactericidal properties.

Test example 4 evaluation of Using effects

The emulsions prepared in example 1 and comparative examples 2 to 4 were used for performance tests, 80 subjects were divided into 4 groups, and skin changes were observed after 3 months of use, and then again after 3 months (i.e., 6 months of use), and the results obtained were as shown in table 4 below:

table 4 emulsion use effect test results

From the results of the tests used for the emulsions in table 4 it can be seen that: 1) from the test results of comparative example 2, it can be seen that the extract of Japanese seaweed has a significant effect on the removal of color spots and the whitening of the whole skin, anti-aging, and when Japanese seaweed is not added, the effect of the emulsion is deteriorated; 2) from the test results of comparative example 3 and comparative example 4, it can be seen that the use effect of the emulsion is deteriorated if the chicory extract, the parsley extract and the broccoli extract are used as key factors for regulating the water-oil balance of the skin, and any one of the raw materials is absent or the addition amount of the three raw materials is not within the range recorded by the invention; 3) it can also be seen from the test results in Table 4 that none of the raw materials in the emulsion preparation process exists in isolation, and all raw materials are combined into a whole in a proper ratio to optimize the emulsion performance.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The skin moistening, moisturizing and repairing emulsion is characterized by comprising the following components in percentage by mass: 0.5 to 2 percent of sodium hyaluronate, 1 to 3 percent of almond extract, 0.5 to 5 percent of zostera marina extract, 2 to 5 percent of Japanese seaweed extract, 1.2 to 4 percent of chicory extract, 0.5 to 3 percent of parsley extract, 1 to 3 percent of broccoli extract, 2 to 3 percent of oat extract, 1 to 4 percent of arginine, 2 to 6 percent of polyalcohol, 0.2 to 0.5 percent of preservative, 0.02 to 0.06 percent of thickening agent, acid-base regulator and the balance of water; wherein the polyhydric alcohol is formed by combining glycerol and butanediol in a mass ratio of 1: 1-3;

the preparation method comprises the following steps:

s1, weighing the raw materials according to mass percentage;

2. The skin moisturizing and repairing emulsion as claimed in claim 1, wherein the components are specifically: 0.8 to 1.6 percent of sodium hyaluronate, 1.2 to 2.6 percent of almond extract, 0.8 to 4.2 percent of zostera marina extract, 2.3 to 4.5 percent of Japanese seaweed extract, 1.5 to 3.2 percent of chicory extract, 1 to 2.5 percent of parsley extract, 1.3 to 2.6 percent of broccoli extract, 2.1 to 2.8 percent of oat extract, 1.4 to 3.2 percent of arginine, 2.6 to 5.2 percent of polyalcohol, 0.2 to 0.5 percent of preservative, 0.02 to 0.06 percent of thickening agent, acid-base regulator and the balance of water.

3. The skin moisturizing and repairing emulsion as claimed in claim 2, wherein the components are specifically: 1.2% of sodium hyaluronate, 1.8% of almond extract, 2.4% of zostera marina extract, 3% of Japanese sea weed extract, 2.1% of chicory extract, 1.7% of parsley extract, 1.8% of broccoli extract, 2.5% of oat extract, 2% of arginine, 3.5% of polyhydric alcohol, 0.3% of preservative, 0.04% of thickening agent, acid-base regulator and the balance of water.

4. The skin moisturizing and repairing lotion according to any one of claims 1 to 3, wherein the preservative is a combination of caprylyl glycol and ethyl hexyl glycerol in a mass ratio of 1.2-1.5: 1.

5. The skin moisturizing and repairing emulsion as claimed in any one of claims 1 to 3, wherein the thickener is a combination of xanthan gum and hydroxyethyl cellulose at a mass ratio of 0.5-1.5: 1.