CN105111278A - Method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis - Google Patents
Method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis Download PDFInfo
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- CN105111278A CN105111278A CN201510668973.7A CN201510668973A CN105111278A CN 105111278 A CN105111278 A CN 105111278A CN 201510668973 A CN201510668973 A CN 201510668973A CN 105111278 A CN105111278 A CN 105111278A
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Abstract
The invention provides a method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis. The novel method for preparing crude phycobiliprotein and crude polysaccharide simultaneously comprises steps as follows: fresh and wet porphyra is taken as a raw material, harvested porphyra yezoensis is washed, drained and subjected to centrifugal dewatering processing, and mixing swelling, wet-method ultrafine grinding performed by a colloid mill combined homogenizer, salting-out and other procedures are performed. According to the method, drying processing of the fresh and wet porphyra in the preparation process of phycobiliprotein is eliminated, a prepared crude phycobiliprotein product contains 34.3%-38.9% of phycobiliprotein on the dry basis, and the yield of phycobiliprotein ranges from 4.59% to 4.71%. The method is suitable for industrial application, the crude phycobiliprotein product prepared from porphyra can be taken as a preparation raw material of high-purity porphyra phycobiliprotein, and the prepared crude polysaccharide can be taken as a preparation raw material of polysaccharide.
Description
Technical field
The present invention relates to the preparation method of a kind of phycobiliprotein and polysaccharide, particularly relate to a kind of method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides.
Background technology
Laver be rhodophyta (
rhodophyta), Porphyra (
porphyra) general name of kelp, be one of most important economic algae.Yezoensis laver (
porphyrayezoensis) and porphyra haitanensis (
porphyrahaitanensis) be China two large laver cultivation kind.Laver high protein, lower fat, containing abundant mineral element and multivitamin, thought one of valuable natural seaweed protective foods by the traditional Chinese medical science.At present, on market, laver converted products is mainly dry laver cake, instant sea sedge and brews seaweed soup, causes the present situation that product is single, added value is low and renewal upgrading is slow, constrains sustainable development and the growth of laver industry.
Dry laver contains the protein of 25 ~ 50%, the carbohydrate of 20 ~ 40%.Phycobiliprotein, polysaccharide are the important component of laver.Phycobiliprotein is a kind of water-soluble, chromoprotein with natural radioactivity, form primarily of phycoerythrin, Phycocyanins, C-and allophycocyanin, its special, safety non-toxic bright in colour, there is antitumor, strengthening immunity, anti-oxidant, anti-ageing biological activity of waiting for a long time, can be used as the photosensitizers of oncotherapy, can be used as immunofluorescence label thing and be applied to the aspects such as particular molecule location, detection and medical diagnosis.Phycobiliprotein has wide potential market at food, medicine and cosmetic field, and the phycobiliprotein product price that current offshore company invests and develops can reach more than $ 100/mg.Laver amylose has immunomodulatory, reducing blood-fat, hypoglycemic, anticoagulation, anti-inflammatory, antifatigue, anti-ageing, radioprotective, resisiting influenza virus, the biological function such as anticancer, also has broad application prospects at food, medicine and cosmetic field.But at present to the exploitation of agar-agar phycobiliprotein and polysaccharide, the general practice is with dry laver for raw material, or carries out subsequent disposal by after fresh wet laver drying.But, only can obtain 1 kilogram of dry laver after the fresh wet laver drying of 10 ~ 13 kilograms, and drying process can consume a large amount of heat energy and produce tankage, also can cause protein denaturation and affect phycobiliprotein extraction and activity.Therefore, directly with fresh wet laver for raw material, the exploitation phycobiliprotein of high added value and polysaccharide new preparation technology have great importance and industrialization prospect.
Summary of the invention
Technical problem to be solved by this invention is the deficiency for existing agar-agar phycobiliprotein and polysaccharide technology of preparing, provide a kind of newly, the yezoensis laver that utilizes that method design is reasonable, workable prepares the method for thick phycobiliprotein and Crude polysaccharides.
Technical problem to be solved by this invention is realized by following technical scheme.The present invention is a kind of method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides, is characterized in, the method key step comprises:
(1) harvested yezoensis laver is carried out clean, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 79.5 ~ 81.2%; In fresh wet laver, add the Lin acid dihydride Jia – sodium hydrate buffer solution of pH8.0, the mass ratio of damping fluid and laver is 0.8 ~ 1:1; Homogenate, to slurry, then by slurry material swelling treatment 8 ~ 10 hours at 38 ~ 42 DEG C, obtains the first material.
(2) the first material is carried out milling treatment of colloid, under 75 ~ 100Mpa, carry out high pressure homogenizer process after then the material after process being added appropriate water dilution, obtain the second material; Second material is carried out centrifugal treating, obtains the first supernatant liquor and the first throw out; In the first supernatant liquor, add ammonium sulfate, then at 4 ~ 10 DEG C, leave standstill 10 ~ 12 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out; The second described throw out is thick phycobiliprotein product; The first described throw out and the second supernatant liquor are Crude polysaccharides.
The method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides of the present invention, preferred technical scheme is as follows further:
1, in step (1): when the fresh wet laver after dewatering can not carry out homogenized in time, be placed under DEG C environment of temperature≤-18 after freeze preservation, then add damping fluid and carry out homogenate.
2, in step (1): carried out by harvested yezoensis laver cleaning, draining, centrifugal dehydration treatment, the fresh wet laver that water content is 80 ~ 81% is obtained.
3, in step (1): the mass ratio of damping fluid and laver is 0.9:1; Homogenate, to slurry, then by slurry material swelling treatment 9 hours at 40 DEG C, obtains the first material.
4, in step (2): during material thin up, the mass ratio of water and the first material is 0.8 ~ 1:3.
5, in step (2): when adding ammonium sulfate in the first supernatant liquor, ammonium sulfate saturation ratio is made to reach 45 ~ 60% gradually.
6, in step (2): the material after milling treatment of colloid carries out high pressure homogenizer process after adding appropriate water dilution under 85 ~ 90Mpa.
7, in step (2): add ammonium sulfate in the first supernatant liquor, then at 4 ~ 6 DEG C, leave standstill 10 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out.
In the present invention: dry matter content analysis can adopt 105 DEG C of constant weight methods; In thick phycobiliprotein product the mensuration of phycoerythrin, Phycocyanins, C-, allophycocyanin content adopt high flood peak (high flood peak. the content [J] of phycobiliprotein in different growing stages porphyra haitanensis. Oceanologia et Limnologia Sinica, method 1993:645-648.): the concentration measuring pure phycobiliprotein by Lowry method, then the absorbancy of mixed algae biliprotein in the maximum absorption wave strong point of each phycobiliprotein component is measured, by the optical extinction coefficient of each phycobiliprotein, calculate the content of each phycobiliprotein in mixed algae biliprotein solution according to Lambert-Beer law.
In the inventive method: harvested yezoensis laver is carried out clean, the object of draining, centrifugal dehydration treatment is impurity in removing laver and reduce the water content of laver, be conducive to the addition reducing swelling solution (Lin acid dihydride Jia – sodium hydrate buffer solution), and improve homogenate, swelling effect.The object of the first material being carried out milling treatment of colloid be preliminary broken laver cell wall to make protein stripping (milling treatment of colloid can make product grading reach 2 ~ 50 μm), make material to utilize follow-up high-pressure homogeneous process simultaneously.Be the fluid properties of adjustment material by the object of the material thin up after milling treatment of colloid, be beneficial to follow-up high-pressure homogeneous process.The object of carrying out the high pressure homogenizer process of 75 ~ 100Mpa be depth crushing laver cell wall to make the abundant stripping of protein (clarifixator process can make product grading reach 0.01 ~ 2 μm), thus reach and improve protein yield effect.
Compared with prior art, the beneficial effect of the inventive method is as follows:
The present invention for raw material with fresh wet laver, adopts modern food development technique, first laver is carried out swelling treatment, changes the weave construction of laver, is beneficial to subsequent wet micronizing (process of colloidal mill associating clarifixator) and makes protein stripping; Then, on the basis of swelling treatment, adopt colloidal mill and high pressure homogenizer coupling to carry out soybean dietary fiber process, thus destruction laver cell wall make the abundant stripping of purple laver protein, to utilize the preparation of follow-up phycobiliprotein.The present invention first with fresh wet laver for raw material, establish the technology of preparing of agar-agar phycobiliprotein based on swelling and soybean dietary fiber (process of colloidal mill associating clarifixator) and polysaccharide, and specify that technological process and the parameter of whole technology.At present, to the exploitation of agar-agar phycobiliprotein and polysaccharide, the general practice carries out subsequent disposal with dry laver for raw material or by after fresh wet laver drying, this only can obtain 1 kilogram of dry laver after causing the fresh wet laver drying of 10 ~ 13 kilograms, and drying process can consume a large amount of heat energy and produce tankage, also can cause protein denaturation and affect phycobiliprotein extraction and activity, therefore present invention, avoiding the shortcoming of this respect, and provide and a kind ofly utilize fresh wet yezoensis laver to prepare the novel method of phycobiliprotein and polysaccharide.Thick phycobiliprotein product prepared by the method contains dry-matter 16.4 ~ 18.9%, containing phycobiliprotein (phycoerythrin+Phycocyanins, C-+allophycocyanin) 34.3 ~ 38.9%(in butt), and phycobiliprotein yield (phycobiliprotein that prepared thick phycobiliprotein product contains account for consumed dehydration after the mass percent of laver) is 4.59 ~ 4.71%.This invention with fresh wet laver for raw material, the operation such as merge swelling, soybean dietary fiber (process of colloidal mill associating clarifixator), saltout, construct the thick phycobiliprotein of laver and Crude polysaccharides novel preparation method, involved colloidal mill and clarifixator are all the equipment that current foodstuffs industry is commonly used, and therefore the present invention is applicable to industrial applications.The first prepared throw out and the second supernatant liquor are Crude polysaccharides, can make laver amylose further; Prepared thick agar-agar phycobiliprotein product can as the raw materials of high-purity laver phycobiliprotein.
Embodiment
Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further.
Embodiment 1, a kind of method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides, the method key step comprises:
(1) harvested yezoensis laver is carried out clean, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 79.5%; In fresh wet laver, add the Lin acid dihydride Jia – sodium hydrate buffer solution of pH8.0, the mass ratio of damping fluid and laver is 0.8:1; Homogenate, to slurry, then by slurry material swelling treatment 8 hours at 38 DEG C, obtains the first material.
(2) the first material is carried out milling treatment of colloid, under 75Mpa, carry out high pressure homogenizer process after then the material after process being added appropriate water dilution, obtain the second material; Second material is carried out centrifugal treating, obtains the first supernatant liquor and the first throw out; In the first supernatant liquor, add ammonium sulfate, then at 4 DEG C, leave standstill 10 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out; The second described throw out is thick phycobiliprotein product; The first described throw out and the second supernatant liquor are Crude polysaccharides.
In the step (2) of the present embodiment: during material thin up, the mass ratio of water and the first material is 0.8:3; When adding ammonium sulfate in the first supernatant liquor, ammonium sulfate saturation ratio is made to reach 45% gradually.
Embodiment 2, a kind of method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides, the method key step comprises:
(1) harvested yezoensis laver is carried out clean, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 81.2%; In fresh wet laver, add the Lin acid dihydride Jia – sodium hydrate buffer solution of pH8.0, the mass ratio of damping fluid and laver is 1:1; Homogenate, to slurry, then by slurry material swelling treatment 10 hours at 42 DEG C, obtains the first material.
(2) the first material is carried out milling treatment of colloid, under 100Mpa, carry out high pressure homogenizer process after then the material after process being added appropriate water dilution, obtain the second material; Second material is carried out centrifugal treating, obtains the first supernatant liquor and the first throw out; In the first supernatant liquor, add ammonium sulfate, then at 10 DEG C, leave standstill 12 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out; The second described throw out is thick phycobiliprotein product; The first described throw out and the second supernatant liquor are Crude polysaccharides.
In the step (2) of the present embodiment: during material thin up, the mass ratio of water and the first material is 1:3; When adding ammonium sulfate in the first supernatant liquor, ammonium sulfate saturation ratio is made to reach 60% gradually.
Embodiment 3, a kind of method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides, the method key step comprises:
(1) harvested yezoensis laver is carried out clean, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 80%; In fresh wet laver, add the Lin acid dihydride Jia – sodium hydrate buffer solution of pH8.0, the mass ratio of damping fluid and laver is 0.9:1; Homogenate, to slurry, then by slurry material swelling treatment 9 hours at 40 DEG C, obtains the first material.
(2) the first material is carried out milling treatment of colloid, under 85Mpa, carry out high pressure homogenizer process after then the material after process being added appropriate water dilution, obtain the second material; Second material is carried out centrifugal treating, obtains the first supernatant liquor and the first throw out; In the first supernatant liquor, add ammonium sulfate, then at 6 DEG C, leave standstill 11 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out; The second described throw out is thick phycobiliprotein product; The first described throw out and the second supernatant liquor are Crude polysaccharides.
In the step (2) of the present embodiment: during material thin up, the mass ratio of water and the first material is 0.9:3; When adding ammonium sulfate in the first supernatant liquor, ammonium sulfate saturation ratio is made to reach 50% gradually.
Embodiment 4, a kind of phycobiliprotein and polyoses producing method utilizing fresh wet yezoensis laver, its key step comprises:
(1) harvested yezoensis laver is carried out clean, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 80.3%; In the fresh wet laver after dehydration, add the Lin acid dihydride Jia – sodium hydrate buffer solution of pH8.0, make the mass ratio of damping fluid and laver be 1:1; Homogenate mixtures, to slurry, then by slurry material swelling treatment 9 hours at 40 DEG C, obtains the first material.
(2) the first material is carried out milling treatment of colloid, then by the material thin up after milling treatment of colloid, make the mass ratio of water and the first material be 1:3, then carry out the high pressure homogenizer process under 80Mpa, obtain the second material; Second material is carried out centrifugal treating, obtains the first supernatant liquor and the first throw out; In the first supernatant liquor, add ammonium sulfate, make ammonium sulfate saturation ratio reach 45% gradually, then at 4 DEG C, leave standstill 12 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out; The second described throw out is thick phycobiliprotein product; The first described throw out and the second supernatant liquor are Crude polysaccharides.
In the present embodiment, when the fresh wet laver after dehydration can not carry out homogenized in time, can put under DEG C environment of temperature≤-18 after freeze preservation, then add damping fluid and carry out homogenate.
Embodiment 5, a kind of phycobiliprotein and polyoses producing method utilizing fresh wet yezoensis laver, its key step comprises:
(1) harvested yezoensis laver is carried out clean, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 80.8%; In the fresh wet laver after dehydration, add the Lin acid dihydride Jia – sodium hydrate buffer solution of pH8.0, make the mass ratio of damping fluid and laver be 0.9:1; Homogenate mixtures, to slurry, then by slurry material swelling treatment 10 hours at 39 DEG C, obtains the first material.
(2) the first material is carried out milling treatment of colloid, then by the material thin up after milling treatment of colloid, make the mass ratio of water and the first material be 0.9:3, then carry out the high pressure homogenizer process under 90Mpa, obtain the second material; Second material is carried out centrifugal treating, obtains the first supernatant liquor and the first throw out; In the first supernatant liquor, add ammonium sulfate, make ammonium sulfate saturation ratio reach 60% gradually, then at 6 DEG C, leave standstill 10 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out; The second described throw out is thick phycobiliprotein product; The first described throw out and the second supernatant liquor are Crude polysaccharides.
In the present embodiment, when the fresh wet laver after dehydration can not carry out homogenized in time, can put under DEG C environment of temperature≤-18 after freeze preservation, then add damping fluid and carry out homogenate.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (8)
1. utilize yezoensis laver to prepare a method for thick phycobiliprotein and Crude polysaccharides, it is characterized in that, the method key step comprises:
(1) harvested yezoensis laver is carried out clean, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 79.5 ~ 81.2%; In fresh wet laver, add the Lin acid dihydride Jia – sodium hydrate buffer solution of pH8.0, the mass ratio of damping fluid and laver is 0.8 ~ 1:1; Homogenate, to slurry, then by slurry material swelling treatment 8 ~ 10 hours at 38 ~ 42 DEG C, obtains the first material;
(2) the first material is carried out milling treatment of colloid, under 75 ~ 100Mpa, carry out high pressure homogenizer process after then the material after process being added appropriate water dilution, obtain the second material; Second material is carried out centrifugal treating, obtains the first supernatant liquor and the first throw out; In the first supernatant liquor, add ammonium sulfate, then at 4 ~ 10 DEG C, leave standstill 10 ~ 12 hours, then carry out centrifugal treating, obtain the second supernatant liquor and the second throw out; The second described throw out is thick phycobiliprotein product; The first described throw out and the second supernatant liquor are Crude polysaccharides.
2. the method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides according to claim 1, it is characterized in that, in step (1): when the fresh wet laver after dewatering can not carry out homogenized in time, be placed under DEG C environment of temperature≤-18 after freeze preservation, then add damping fluid and carry out homogenate.
3. the method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides according to claim 1, it is characterized in that, in step (1): carried out by harvested yezoensis laver cleaning, draining, centrifugal dehydration treatment, obtain the fresh wet laver that water content is 80 ~ 81%.
4. the method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides according to claim 1, is characterized in that, in step (1): the mass ratio of damping fluid and laver is 0.9:1; Homogenate, to slurry, then by slurry material swelling treatment 9 hours at 40 DEG C, obtains the first material.
5. the method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides according to claim 1, is characterized in that, in step (2): during material thin up, the mass ratio of water and the first material is 0.8 ~ 1:3.
6. the method utilizing yezoensis laver to prepare thick phycobiliprotein and Crude polysaccharides according to claim 1, is characterized in that, in step (2): when adding ammonium sulfate in the first supernatant liquor, makes ammonium sulfate saturation ratio reach 45 ~ 60% gradually.
7. utilize yezoensis laver to prepare the method for thick phycobiliprotein and Crude polysaccharides according to claim 1 or 5, it is characterized in that, in step (2): the material after milling treatment of colloid carries out high pressure homogenizer process after adding appropriate water dilution under 85 ~ 90Mpa.
8. the yezoensis laver that utilizes according to claim 1 or 6 prepares the method for thick phycobiliprotein and Crude polysaccharides, it is characterized in that, in step (2): add ammonium sulfate in the first supernatant liquor, then at 4 ~ 6 DEG C, 10 hours are left standstill, carry out centrifugal treating again, obtain the second supernatant liquor and the second throw out.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107141342A (en) * | 2017-06-21 | 2017-09-08 | 江苏中兴药业有限公司 | A kind of method that soybean dietary fiber method extracts silybum marianum seeds albumen |
| CN107722132A (en) * | 2017-10-23 | 2018-02-23 | 青岛大学 | A kind of method of coproduction algal polysaccharides and Sargassum protein from marine alga |
| CN107853681A (en) * | 2017-11-18 | 2018-03-30 | 淮海工学院 | A kind of method that plural gel body is prepared using seaweed and asparagus |
| CN109400745A (en) * | 2018-11-21 | 2019-03-01 | 温州大学苍南研究院 | A kind of preparation of low molecular weight Porphyra yezoensis Polysaccharides and its application in anti-human cervical cancer cell tumour |
| CN109608514A (en) * | 2018-11-29 | 2019-04-12 | 河北中科汉禧生物科技有限公司 | The method of protein and polysaccharide is extracted from turnip |
| CN111411090A (en) * | 2020-04-07 | 2020-07-14 | 珀莱雅化妆品股份有限公司 | Preparation method and application of high-temperature-resistant superoxide dismutase |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617784A (en) * | 2008-07-01 | 2010-01-06 | 宋俊文 | Method for preparing eatable phycobiliprotein in laver |
| CN104447967A (en) * | 2014-11-26 | 2015-03-25 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori |
| CN104672325A (en) * | 2015-03-11 | 2015-06-03 | 福建农林大学 | Method for preparing phycocyanin from fresh spirulina |
| CN104844707A (en) * | 2015-05-28 | 2015-08-19 | 江西三达新大泽生物工程有限公司 | Method for extracting phycocyanobilin from spirulina |
-
2015
- 2015-10-13 CN CN201510668973.7A patent/CN105111278A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617784A (en) * | 2008-07-01 | 2010-01-06 | 宋俊文 | Method for preparing eatable phycobiliprotein in laver |
| CN104447967A (en) * | 2014-11-26 | 2015-03-25 | 南通中国科学院海洋研究所海洋科学与技术研究发展中心 | Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori |
| CN104672325A (en) * | 2015-03-11 | 2015-06-03 | 福建农林大学 | Method for preparing phycocyanin from fresh spirulina |
| CN104844707A (en) * | 2015-05-28 | 2015-08-19 | 江西三达新大泽生物工程有限公司 | Method for extracting phycocyanobilin from spirulina |
Non-Patent Citations (4)
| Title |
|---|
| 李玉环主编: "《水产品加工技术（第二版）》", 30 September 2014, 中国轻工业出版社 * |
| 肖海芳等: "条斑紫菜蛋白和多糖提取工艺优化", 《食品研究与开发》 * |
| 蔡春尔等: "硫酸铵三步盐析对藻胆蛋白纯化的影响", 《生物技术通报》 * |
| 邹礼根等主编: "《农产品加工副产物综合利用技术》", 31 March 2013, 浙江大学出版社 * |
Cited By (8)
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|---|---|---|---|---|
| CN107141342A (en) * | 2017-06-21 | 2017-09-08 | 江苏中兴药业有限公司 | A kind of method that soybean dietary fiber method extracts silybum marianum seeds albumen |
| CN107141342B (en) * | 2017-06-21 | 2020-06-23 | 江苏中兴药业有限公司 | Method for extracting silybum marianum seed kernel protein by wet superfine grinding method |
| CN107722132A (en) * | 2017-10-23 | 2018-02-23 | 青岛大学 | A kind of method of coproduction algal polysaccharides and Sargassum protein from marine alga |
| CN107853681A (en) * | 2017-11-18 | 2018-03-30 | 淮海工学院 | A kind of method that plural gel body is prepared using seaweed and asparagus |
| CN109400745A (en) * | 2018-11-21 | 2019-03-01 | 温州大学苍南研究院 | A kind of preparation of low molecular weight Porphyra yezoensis Polysaccharides and its application in anti-human cervical cancer cell tumour |
| CN109400745B (en) * | 2018-11-21 | 2021-01-05 | 温州大学苍南研究院 | Preparation of low molecular weight porphyra yezoensis polysaccharide and application of low molecular weight porphyra yezoensis polysaccharide in resisting human cervical cancer cell tumor |
| CN109608514A (en) * | 2018-11-29 | 2019-04-12 | 河北中科汉禧生物科技有限公司 | The method of protein and polysaccharide is extracted from turnip |
| CN111411090A (en) * | 2020-04-07 | 2020-07-14 | 珀莱雅化妆品股份有限公司 | Preparation method and application of high-temperature-resistant superoxide dismutase |
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Application publication date: 20151202 |