CN104447967A - Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori - Google Patents
Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori Download PDFInfo
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Abstract
The invention provides a method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori. The method comprises the following steps: firstly, extracting phycoerythrin from fresh inferior nori; then, extracting sulphated porphyra polysaccharide from residues of phycoerythrin; and simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide in a primary extraction process. The method provided by the invention can be used for obtaining phycoerythrin and sulphated porphyra polysaccharide in primary extraction. The obtained phycoerythrin is high in purity and the sulphated porphyra polysaccharide is good in uniformity. According to the method provided by the invention, the operation is simple and convenient, the process is simple, the separating speed is high, the cost is low, the utilization efficiency of inferior nori is improved, and resources are repeatedly used and utilized for many times.
Description
Technical field
The Extraction and isolation of algae functional mass of the present invention, is specifically related to a kind of method simultaneously extracting phycoerythrin and laver sulfated polysaccharide from laver.
Background technology
Laver is the kelp that a class has Important Economic value, has several kinds extensively to cultivate the coastal various countries in South East Asia at present.According to statistics, laver annual production in 2006 about 1.8 × 10
4ton dry product, annual value of production estimates about 1,300,000,000 dollars (FAO, 2006).China's laver industry development is rapid, from distributed areas, based on porphyra haitanensis on the south the Changjiang river, mainly concentrates on Fujian Province and Guangdong Province; North of Yangtze River, based on yezoensis laver, mainly concentrates on Jiangsu Province and Shandong Province.Wherein the whole province of Jiangsu Province laver cultivated area about 330,000 mu, practitioner about 80,000, and in Nantong, Yancheng and sea area, Lianyun Harbour define the main producing region of China's yezoensis laver, annual production 4000000000 pieces of standardized products, the industry gross output value about 2,000,000,000 yuan.The laver produced once with after secondary processing forms product in locality, sells to all over the world, becomes the important component part that China's export is earned foreign exchange.
In yezoensis laver is produced, inferior laver is because of the reason that glue content is high, protein content is low, and cause time processing quality product on the low side, secondary processing product mouthfeel is bad, thus on the low side, and then affects Business Economic Benefit.These inferior lavers usually together with the discarded laver produced in the course of processing as refuse treatment, not only waste resource, turn increase environmental pressure.Therefore, the high-valued processing of these inferior lavers and the extraction of high added value material thereof have become the key of laver industry Sustainable development.
Phycobiliprotein is a kind of water colo(u)r albumen, and have unique absorption spectrum and fluorescence emission spectrum, its existence supplements chlorophyllous light abstraction width, has widened the absorption of algae to visible ray, has more been conducive to the adaptation to aquatic environment.R-PE is I type R-PE, due to the spectral response curve of its uniqueness, stability and higher uptake factor and quantum yield, phycobiliprotein can be used as the fluorescent probe of Biochemical Research and apply to the macromolecular mark of Cell and organism, also can be used as the photosensitizers of oncotherapy, even report that it has immunoregulatory effect.In addition, phycobiliprotein also can be used as the interpolation of natural pigment for food, makeup, avoids the toxicity hazard that chemosynthesis pigment may bring.
Laver amylose is one of its cell main ingredient, has the effects such as anticoagulation, reducing blood-fat, anti-ageing and immunomodulatory.There is report display laver amylose by reducing mouse MDA level, directly can remove interior free yl and improving its resistance of oxidation.Thus laver amylose is the important component of a kind of more satisfactory be developed to antisenility cistanche food and medicine.There is great amount of hydroxy group or carboxyl isopolarity group in polysaccharide molecule, hydrogen bond can be formed with water molecules, show good moisture absorption and performance of keeping humidity, so also have broad application prospects in medicine, makeup, agricultural etc.Separable to there is activating macrophage, strengthening the polysaccharide fraction of immunologic function from yezoensis laver.
So people expect utilizing laver to extract phycobiliprotein and laver amylose simultaneously, done large quantity research for this reason.
Xiao Haifang etc. explore pulse ultrasonic wave and extract the technique of albumen and polysaccharide in laver simultaneously (pulse ultrasonic wave extracts the technical study of albumen and polysaccharide in laver simultaneously, Food science, 2007, Vol.28, No.06), Zhu Xiaojun etc. explore the technique (optimization of synchronous ultrasonic-assisted extraction Porphyra yezoensis Polysaccharides and phycobiliprotein technique of simultaneously extracting laver amylose and phycobiliprotein with ultrasonic power, Food science, 2008, Vol.29, No.05).Chinese patent CN200810018871.0 discloses a kind of Porphyra yezoensis Polysaccharides and protein synchronous ultrasonic auxiliary extraction method.But these extraction processes are all utilize laver dry powder, the phycobiliprotein spectrum activity obtained is very low, and polysaccharide is inevitably subject to the pollution of other molecules such as albumen in leaching process, complicated component.
Summary of the invention
From inferior laver, the method for phycoerythrin and laver sulfated polysaccharide is extracted the while of the object of the present invention is to provide a kind of, the phycoerythrin purity obtained is high, polysaccharide is contaminated little, extraction process is simple, improves inferior laver resource utilization, improves the economic benefit of laver industry.
For achieving the above object, the present invention adopts technical scheme to be:
A kind of method of simultaneously separation and Extraction phycoerythrin and sulfated polysaccharide from inferior laver, first phycoerythrin is extracted from fresh inferior laver, from the residue being extracted phycoerythrin, extract laver sulfated polysaccharide again, in a leaching process, extract phycoerythrin and laver sulfated polysaccharide simultaneously.
Wherein, concrete steps are as follows:
A. phycoerythrin extracts
(1) by fresh inferior laver chopping, put into 1% phosphate buffered saline buffer and make that its cell is swelling to break;
(2) centrifugal, collect supernatant liquor, obtain phycoerythrin crude extract, adding solid ammonium sulfate to final concentration is 0.5M;
(3) phycoerythrin crude extract is pumped into Phenyl-Sepharose post, foreign protein is removed in cleaning;
(4) use ammoniumsulphate soln, distilled water from Phenyl-Sepharose post wash-out phycoerythrin successively;
(5) elutriant of gained in step (4) is added DEAE-Sepharose ion exchange column respectively, first wash post with 50mM, 100mM phosphate buffered saline buffer, 10mM sodium-acetate, 4mM acetic acid successively, then with the phosphate buffered saline buffer wash-out containing NaCl, the phycoerythrin of purifying is obtained;
B. from residue, laver sulfated polysaccharide is extracted
(1) remaining residue after utilization extraction phycoerythrin, under 10 times of volume 1% phosphate buffered saline buffers, pH6.0 acidity, control temperature, at 110 ~ 121 DEG C, extracts 1 hour, filtered through gauze; In filter residue, add 1% phosphate buffered saline buffer of 5 times of volumes, repeat aforesaid operations, again carry out extracting, filtering; Then merge twice filtrate, obtain liquid of extracting polysaccharide; Liquid of extracting polysaccharide rotary evaporation is concentrated;
(2) in the liquid of extracting polysaccharide after concentrated, add ethanol, collecting precipitation, lyophilize, obtain laver sulfated polysaccharide dry powder.
Wherein, the pH=6.0 of 1% phosphate buffered saline buffer in steps A (1).
Wherein, centrifugal in steps A (2) be rotating speed be 9000 ~ 11000g, temperature be 5 ~ 10 DEG C of conditions under centrifugal 10 minutes.
Wherein, steps A (4) be use 0.20 successively, 0.10,0.05M ammoniumsulphate soln, distilled water with the speed of 3 ~ 5ml/min from Phenyl-Sepharose post wash-out phycoerythrin.
Wherein, use described in steps A (5), containing the phosphate buffered saline buffer wash-out of NaCl, is successively with 50mM phosphate buffered saline buffer, the 50mM phosphate buffered saline buffer containing 0.20M NaCl, the 100mM phosphate buffered saline buffer wash-out containing 0.2M NaCl containing 0.15M NaCl.
Wherein, step B (2) is that the ethanol adding 4 times of volumes precipitates.
Laver frond is pulverized by the present invention, water-soluble substances extracts with the expanded post of Phenyl-Sepharose under finite concentration salt ion condition, phycoerythrin goods, with DEAE-Sepharose ion exchange column, purifying is carried out to phycoerythrin again, phycoerythrin sterling can be obtained, then the remaining residue of extraction phycoerythrin is used for the extraction of laver sulfated polysaccharide.After purifying, phycoerythrin spectral purity is greater than 3.2, and productive rate is 0.66mg/g; Gained sulfated polysaccharide productive rate is 32.9mg/g.
The active phycoerythrin of the first purifying of the present invention, then extract sulfated polysaccharide, reaches the object of comprehensive utilization biomass, meanwhile, due to the extraction of phycobiliprotein, reduces the pollution of the carbohydrates such as the pollution of albumen when sulfated polysaccharide extracts and starch.Therefore, present method not only can obtain spectral purity higher than the SILVER REAGENT phycoerythrin of 3.2, and can obtain the good sulfated polysaccharide of homogeneity simultaneously.
Tool of the present invention has the following advantages:
1. operating process is simple.Extract raw material and only need chopping, freeze thawing, filter, obtain crude extract.Phycobiliprotein does not need to carry out pre-treatment before being separated.
2. the residue after the present invention protein extraction carries out Polyose extraction, reaches the object making full use of resource on the one hand, on the other hand after protein extraction, decreases protein contamination during Polyose extraction, kills two birds with one stone.
3. phycobiliprotein velocity of separation is fast, and a complete sepn process only needs 3.5 hours, and comprise required 30 minutes of the balance of post, loading 60 minutes, cleans 50 minutes, wash-out 60 minutes.
4. the present invention proposes a kind of method of laver comprehensive utilization of simple and effective, phycoerythrin spectral purity after purifying is greater than 3.2, and productive rate is 0.66mg/g, and sulfated polysaccharide yield is 32.9mg/g, polysaccharide homogeneity is good, greatly can be improved the utilization ratio of laver by the present invention.
The present invention utilizes inferior laver to extract phycobiliprotein and laver sulfated polysaccharide simultaneously, has gained phycoerythrin spectral purity high, the contaminated little advantage of laver sulfated polysaccharide.Easy and simple to handle, technique is simple, and velocity of separation is fast, and cost is low, improves the utilising efficiency of inferior laver, achieves reusing and repeatedly utilizing of resource.
Accompanying drawing explanation
Fig. 1 is the absorption spectrum of yezoensis laver crude extract at 250-800nm.
Fig. 2 A is 0.2M ammoniumsulphate soln from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
Fig. 2 B is 0.1M ammoniumsulphate soln from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
Fig. 2 C is 0.05M ammoniumsulphate soln from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
Fig. 2 D is distilled water from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
Fig. 3 A is phycoerythrin absorption spectrum at 250-800nm after ion exchange column purification.
Fig. 3 B is phycoerythrin fluorescence emission spectrum after ion-exchange resin purification.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read content of the present invention, these equivalent form of values fall within limited range of the present invention equally.
Embodiment 1, the extraction of phycoerythrin and purification process
1., after taking the fresh inferior laver chopping of 100g weight in wet base, add 10 times to 1% phosphate buffered saline buffer of frond weight, 4 DEG C are spent the night, and make cell swelling.
2. rotating speed be 9000g, temperature be 5 ~ 10 DEG C of conditions under centrifugal 10 minutes, collect whole supernatant liquor, obtain phycoerythrin crude extract 340ml.Collect all residues to put into-20 DEG C of refrigerators and preserve, in order to carrying out the extraction of sulfated polysaccharide.
Measure the OD value of phycoerythrin crude extract, then according to formula R-PE=155.8OD498.5-40.0OD614-10.5OD651, calculate containing 53.4mg phycoerythrin in crude extract, productive rate is 5.34mg/g.
Fig. 1 is the absorption spectrum of R-PE crude extract at 250-800nm.
The expanded post of 3.Pheny-Sepharose extracts.First with the expanded post of 0.5M ammoniumsulphate soln balance Pheny-Sepharose.At room temperature, adding solid ammonium sulfate to final concentration at phycoerythrin crude extract is 0.5M, and pump into expanded post from bottom to top with the speed of 15ml/min, phycobiliprotein is caught by sorbent material.Rinse pillar with 0.5M ammoniumsulphate soln again, impurity and cell debris are rinsed well, until effluent liquid does not have color.
4. use 0.20 successively, 0.10,0.05M ammoniumsulphate soln and distilled water is with the speed of 15ml/min elution phycoerythrin from the top down.Collect whole eluant, after dialysis, measure its volume, measure the absorption spectrum of 250-800nm.
Fig. 2 A is 0.2M ammoniumsulphate soln from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
Fig. 2 B is 0.1M ammoniumsulphate soln from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
Fig. 2 C is 0.05M ammoniumsulphate soln from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
Fig. 2 D is distilled water from absorption spectrum at 250-800nm of the phycoerythrin of the expanded post elution of Pheny-Sepharose.
0.20,0.10,0.05M ammoniumsulphate soln from the purity of expanded post elution samples and content in table 1.Total protein content is 91.2mg, and productive rate is 0.912mg/g.
The phycoerythrin yield of the different ammonium sulfate concentrations wash-out of table 1 and purity
5.DEAE-Sepharose ion exchange column purification.The eluant of upper step gained is pumped into DEAE-Sepharose ion exchange column respectively, 50mM, 100mM phosphoric acid buffer (PH 6.8) is first used to wash post, post is washed again with 10mM sodium-acetate, post washed by 4mM acetic acid, then adds 0.15M NaCl, 50mM phosphoric acid buffer with 50mM phosphoric acid buffer respectively and adds 0.20MNaCl, 100mM phosphoric acid buffer and add 0.20M NaCl solution wash-out.Collect red elutriant, the absorption spectrum of measurement volumes, mensuration 250-800nm.
Fig. 3 A is phycoerythrin absorption spectrum at 250-800nm after ion exchange column purification.
Fig. 3 B is phycoerythrin fluorescence emission spectrum after ion-exchange resin purification.
Different concns phosphate buffered saline buffer and sodium-chlor from the purity of the sample of ion exchange column wash-out and content in table 2.Phycoerythrin spectral purity after purifying is greater than 3.2, and total protein concentration is 66mg, and productive rate is 0.66mg/g.
The phycoerythrin yield of purifying and purity under the different salt ion condition of table 2
Embodiment 2, the extraction of sulfated polysaccharide
The residue of gained after extraction phycoerythrin crude extract is put into Erlenmeyer flask, and add 10 times of volume 1% phosphate buffered saline buffers that pH is 6.0, control temperature boils 1 hour at 110 ~ 121 DEG C, then 8 layers of filtered through gauze; In filter residue, again add 1% phosphoric acid buffer of 5 times of volumes, pH 6.0, under uniform temp condition, boil 1 hour again; Filter, merge twice filtrate, namely obtain liquid of extracting polysaccharide; By liquid of extracting polysaccharide rotary evaporation to 1/4th of original volume; Add 4 times of volume alcohol, collecting precipitation, freeze-drying.The results are shown in Table 3.
Laver amylose yield and sulfate content under table 3 various extracting conditions
Visible, extracting the red rear residual residue of algae with fresh frond weight in wet base 100g is raw material, can obtain sulfated polysaccharide 3.29 grams, productive rate 3.29%.
In leaching process, liquid of extracting polysaccharide, after concentrated, adds 3% trichoroacetic acid(TCA), 4 DEG C of hold over night, centrifugal, is not precipitated, illustrates that in liquid of extracting polysaccharide, protein contamination is little.
The ethanol of 2 times, 4 times and 8 times volumes is added successively, 4 DEG C of hold over night in liquid of extracting polysaccharide, centrifugal, Fraction collection polysaccharide precipitation.The polysaccharide precipitation that 2 times of volumes obtain accounts for the overwhelming majority, and the polysaccharide amount that 4 times of volumes obtain is relatively low, can not get polysaccharide precipitation during 8 times of volume ethanol postincubation.Illustrate that 4 times of volume ethanol can be substantially complete by polysaccharide precipitation.
The present invention can obtain phycoerythrin and laver sulfated polysaccharide in once extracting, and the spectral purity of the phycoerythrin obtained is high, and laver sulfated polysaccharide is without protein contamination, and homogeneity is good.The present invention is easy and simple to handle, and technique is simple, and velocity of separation is fast, and cost is low, improves the utilising efficiency of inferior laver, achieves the comprehensive utilization of resource.
Be more than the description to the embodiment of the present invention, by the above-mentioned explanation to the disclosed embodiments, professional and technical personnel in the field realized or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (6)
1. one kind is extracted the method for phycoerythrin and laver sulfated polysaccharide from inferior laver simultaneously, it is characterized in that, first phycoerythrin is extracted from fresh inferior laver, from the residue being extracted phycoerythrin, extract laver sulfated polysaccharide again, in a leaching process, extract phycoerythrin and laver sulfated polysaccharide simultaneously.
2., by the method extracting phycoerythrin and laver sulfated polysaccharide from inferior laver according to claim 1 simultaneously, it is characterized in that, concrete steps are as follows:
A. phycoerythrin extracts
(1) by fresh inferior laver chopping, put into 1% phosphate buffered saline buffer and make that its cell is swelling to break;
(2) centrifugal, collect supernatant liquor, obtain phycoerythrin crude extract, adding solid ammonium sulfate to final concentration is 0.5M;
(3) phycoerythrin crude extract is pumped into Phenyl-Sepharose post, foreign protein is removed in cleaning;
(4) use ammoniumsulphate soln, distilled water from Phenyl-Sepharose post wash-out phycoerythrin successively;
(5) elutriant of gained in step (4) is added DEAE-Sepharose ion exchange column respectively, first wash post with 50mM, 100mM phosphate buffered saline buffer, 10mM sodium-acetate, 4mM acetic acid respectively, then with the phosphate buffered saline buffer wash-out containing NaCl, the phycoerythrin of purifying is obtained;
B. from residue, laver sulfated polysaccharide is extracted
(1) remaining residue after utilization extraction phycoerythrin, under 10 times of volume 1% phosphate buffered saline buffers, pH6.0 acidity, control temperature, at 110 ~ 121 DEG C, extracts 1 hour, filtered through gauze; In filter residue, add 1% phosphate buffered saline buffer of 5 times of volumes, repeat aforesaid operations, again carry out extracting, filtering; Then merge twice filtrate, obtain liquid of extracting polysaccharide; Liquid of extracting polysaccharide rotary evaporation is concentrated;
(2) in the liquid of extracting polysaccharide after concentrated, add ethanol, collecting precipitation, lyophilize, obtain laver sulfated polysaccharide dry powder.
3., by a kind of method extracting phycoerythrin and laver sulfated polysaccharide from laver according to claim 2 simultaneously, it is characterized in that, centrifugal in steps A (2) be rotating speed be 9000 ~ 11000g, temperature be 5 ~ 10 DEG C of conditions under centrifugal 10 minutes.
4. by a kind of method extracting phycoerythrin and laver sulfated polysaccharide from laver according to claim 2 simultaneously, it is characterized in that, steps A (4) be use 0.20 successively, 0.10,0.05M ammoniumsulphate soln, distilled water with the speed of 3 ~ 5ml/min from Phenyl-Sepharose post wash-out phycoerythrin.
5. by the method extracting phycoerythrin and laver sulfated polysaccharide from laver according to claim 2 simultaneously, it is characterized in that, use described in steps A (5), containing the phosphate buffered saline buffer wash-out of NaCl, is successively with 50mM phosphate buffered saline buffer, the 50mM phosphate buffered saline buffer containing 0.20MNaCl, the 100mM phosphate buffered saline buffer wash-out containing 0.2M NaCl containing 0.15M NaCl.
6. by a kind of method extracting phycoerythrin and laver sulfated polysaccharide from laver according to claim 2 simultaneously, it is characterized in that, step B (2) is that the ethanol adding 4 times of volumes precipitates.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111278A (en) * | 2015-10-13 | 2015-12-02 | 淮海工学院 | Method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis |
| CN106117326A (en) * | 2015-12-09 | 2016-11-16 | 烟台大学 | A kind of centrifuging combines the method that anion-exchange chromatography medium prepares phycoerythrin |
| CN106117347A (en) * | 2015-12-09 | 2016-11-16 | 烟台大学 | A kind of hydrophobic chromatography prepares the method for high-purity phycoerythrin |
| CN106146631A (en) * | 2015-12-09 | 2016-11-23 | 烟台大学 | The method that a kind of centrifugation technique bonded hydrophobic layer analysis medium prepares phycoerythrin |
| CN107312076A (en) * | 2017-08-01 | 2017-11-03 | 中国科学院海洋研究所 | A kind of method that phycoerythrin is extracted in the dry product from Porphyra yezoensis |
| CN109965173A (en) * | 2019-03-15 | 2019-07-05 | 湖州师范学院 | A kind of preparation method and applications for extracting rhodophyll, polysaccharide and dietary fiber from low valued laver |
| CN112646051A (en) * | 2021-01-20 | 2021-04-13 | 河南理工大学 | Extraction method of callicarpa kwangtungensis chun polysaccharide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1106414A (en) * | 1994-08-24 | 1995-08-09 | 沈阳医药工业研究所 | Method for extracting protein and polysaccharide from spirulina and application thereof |
-
2014
- 2014-11-26 CN CN201410697186.0A patent/CN104447967A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1106414A (en) * | 1994-08-24 | 1995-08-09 | 沈阳医药工业研究所 | Method for extracting protein and polysaccharide from spirulina and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 牛建峰: "海洋藻类功能性蛋白质的纯化、鉴定及其应用的研究", 《中国博士学位论文全文数据库 基础科学辑》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111278A (en) * | 2015-10-13 | 2015-12-02 | 淮海工学院 | Method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis |
| CN106117326A (en) * | 2015-12-09 | 2016-11-16 | 烟台大学 | A kind of centrifuging combines the method that anion-exchange chromatography medium prepares phycoerythrin |
| CN106117347A (en) * | 2015-12-09 | 2016-11-16 | 烟台大学 | A kind of hydrophobic chromatography prepares the method for high-purity phycoerythrin |
| CN106146631A (en) * | 2015-12-09 | 2016-11-23 | 烟台大学 | The method that a kind of centrifugation technique bonded hydrophobic layer analysis medium prepares phycoerythrin |
| CN107312076A (en) * | 2017-08-01 | 2017-11-03 | 中国科学院海洋研究所 | A kind of method that phycoerythrin is extracted in the dry product from Porphyra yezoensis |
| CN109965173A (en) * | 2019-03-15 | 2019-07-05 | 湖州师范学院 | A kind of preparation method and applications for extracting rhodophyll, polysaccharide and dietary fiber from low valued laver |
| CN109965173B (en) * | 2019-03-15 | 2022-09-13 | 湖州师范学院 | Preparation method and application of phycoerythrin, polysaccharide and dietary fiber extracted from laver in water powder |
| CN112646051A (en) * | 2021-01-20 | 2021-04-13 | 河南理工大学 | Extraction method of callicarpa kwangtungensis chun polysaccharide |
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Application publication date: 20150325 |