CN113855597A

CN113855597A - Antioxidant, anti-inflammatory and soothing repair composition, preparation method and application thereof

Info

Publication number: CN113855597A
Application number: CN202111148109.6A
Authority: CN
Inventors: 刘慧敏; 荀梦涵; 王伟; 刘清雷; 雷胜楠; 赵志伟; 程明艳; 汤伟
Original assignee: Shanghai Institute of Technology
Current assignee: Shanghai Institute of Technology
Priority date: 2021-09-29
Filing date: 2021-09-29
Publication date: 2021-12-31

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an antioxidant, anti-inflammatory and soothing repair composition, a preparation method and application thereof, wherein the antioxidant, anti-inflammatory and soothing repair composition comprises the following components in parts by weight: 16-24 parts of cactus extract, 3.6-6 parts of licorice extract and 0.96-1.6 parts of nymphaea hybrid extract. The composition provided by the invention has obvious antioxidant, anti-inflammatory and repairing effects, and is safe in components and free of adverse reactions. The results of the examples show that the free radical clearance rate can reach 99.95%, the hyaluronidase inhibition rate can reach 100%, the migration of HaCaT is obviously promoted, and the healing rate of the scratch of HaCaT cells can reach 73.92 +/-3.68%.

Description

Antioxidant, anti-inflammatory and soothing repair composition, preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an antioxidant, anti-inflammatory and soothing repair composition, a preparation method and application thereof.

Background

With the improvement of living standard of people and the rapid development of cosmetic industry, the demand of people on cosmetics is increasing day by day, and the shortage of professional knowledge easily causes people to be unable to select cosmetics suitable for the people from cosmetic raw materials and components in shape and color, thereby causing the phenomena of skin allergy, redness and the like; meanwhile, environmental factors such as air pollution and ultraviolet radiation can also cause a large amount of free radicals to be generated by the human body so as to cause skin allergy or inflammation.

The radical is also referred to as "radical" chemically, and refers to an atom or a group having an unpaired electron formed by homolytic cleavage of a covalent bond in a molecule of a compound under external conditions such as photo-thermal conditions. The skin-care product can attack normal cells in a human body, so that the cells are aged and killed, skin barriers are damaged, excessive skin moisture is lost, the environment in the survival of the cells is affected, the intercellular balance and stability are seriously damaged, finally, the resistance of the skin is reduced, skin diseases such as dryness, allergy, bacterial infection, eczema, dermatitis and the like appear, and the daily life quality of people is seriously affected.

At present, the antioxidant, free radical scavenging, anti-allergy, soothing and repairing composition and the cosmetics on the market have unobvious effects, are mostly chemical raw materials, and have obvious side effects. Therefore, the development of natural active substances with antioxidant, anti-inflammatory and soothing and repairing functions and the safe application of the natural active substances to cosmetics to achieve the effects of antioxidant, anti-allergy and soothing and repairing are the problems to be solved in the technical field.

Disclosure of Invention

The invention aims to provide an antioxidant, anti-inflammatory and soothing repair composition, a preparation method and application thereof.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides an antioxidant, anti-inflammatory and soothing repair composition which comprises the following components in parts by weight: 16-24 parts of cactus extract, 3.6-6 parts of licorice extract and 0.96-1.6 parts of nymphaea hybrid extract.

Preferably, the composition further comprises the following components in parts by weight: 0.8-2.4 parts of oat peptide, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

More preferably, the composition comprises the following components in parts by weight: 16 parts of cactus extract, 3.6 parts of licorice extract, 2.4 parts of oat peptide, 1.6 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

The invention provides a preparation method of an antioxidant, anti-inflammatory and soothing repair composition, which comprises the following steps:

respectively soaking radix et caulis Opuntiae Dillenii, Glycyrrhrizae radix and herba Hylothelospermi Japonici in water, ultrasonic extracting to obtain supernatant, concentrating, and lyophilizing to obtain radix et caulis Opuntiae Dillenii extract, Glycyrrhrizae radix extract and herba Hylothelospermi flower extract;

mixing the cactus extract, the licorice extract and the nymphaea hybrid extract to obtain the antioxidant, anti-allergy and soothing repair composition.

Preferably, when the antioxidant, anti-inflammatory and soothing repair composition further comprises avenin, rosmarinic acid and trehalose, the preparation method further comprises:

sequentially crushing oat grains, degreasing, and ultrasonically extracting with Tris-HCl buffer solution to obtain supernatant; mixing the supernatant with trichloroacetic acid with the same volume, and standing to obtain a standing supernatant; sequentially concentrating and freeze-drying the standing supernatant to obtain oat peptide;

mixing the cactus extract, the licorice extract, the nymphaea hybrid extract, the oat peptide, the trehalose and the rosmarinic acid to obtain the antioxidant, anti-allergy and soothing repair composition.

Preferably, the concentrating comprises: concentrating the clear cactus palms, the clear nymphaea hybrid supernatant and the clear oat peptide supernatant to 1/3-1/4 corresponding to the original volume respectively;

and concentrating the liquorice supernatant added with trichloroacetic acid to 1/4-1/5 of the original liquid volume.

Preferably, the degreasing solvent comprises n-hexane; the concentration of the Tris-HCl buffer solution is 0.1 mol/L; the mass percentage concentration of the trichloroacetic acid is 12 percent.

The invention also provides application of the antioxidant, anti-inflammatory and soothing repair composition in preparing cosmetics with antioxidant, anti-inflammatory and soothing repair functions.

Preferably, the antioxidant, anti-inflammatory and soothing repair composition accounts for 0.05-20% of the total mass of the cosmetic.

More preferably, the antioxidant, anti-inflammatory and soothing repair composition accounts for 1.0-3.0% of the total mass of the cosmetic.

The invention has the beneficial effects that:

the invention provides an antioxidant, anti-inflammatory and soothing repair composition which comprises the following components in parts by weight: 16-24 parts of cactus extract, 3.6-6 parts of liquorice extract and 0.96-1.6 parts of nymphaea hybrid extract. The antioxidant, anti-inflammatory and soothing repair composition provided by the invention is mostly derived from green herbaceous plants, belongs to mild active substances, has obvious antioxidant, anti-inflammatory and soothing repair effects, is safe and non-irritant, and has no adverse reaction.

The free radical scavenging experiment, the hyaluronidase inhibition experiment and the cell repair activity evaluation experiment prove that the composition can effectively scavenge excessive free radicals in an organism, and simultaneously can relieve local inflammation, calm and relieve skin, repair the function of damaged skin barrier, assist skin self-repair and maintain skin health. The free radical clearance rate can reach 99.95 percent, the hyaluronidase inhibition rate can reach 100 percent, the HaCaT migration is obviously promoted, and the healing rate of the scratch of the HaCaT cell can reach 73.92 +/-3.68 percent.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments will be briefly described below.

FIG. 1 is a graph showing the results of the effect on HaCaT cell proliferation after 24h treatment with different concentrations of the composition of example 10 of the present invention;

FIG. 2 is a graph showing the results of the effect on HaCaT cell proliferation 48h after treatment with different concentrations of the composition of example 10 of the present invention;

FIG. 3 is a graph showing the results of HaCaT cell migration after treatment with different concentrations of the composition of example 10 for 0h, 12h and 24h, respectively.

Detailed Description

The antioxidant, anti-inflammatory and soothing repair composition provided by the invention preferably further comprises the following components in parts by weight: 0.8-2.4 parts of oat peptide, 0.8 part of rosemary and 0.8 part of trehalose.

In the present invention, the source of each component is not particularly limited, and a conventional commercially available product may be used unless otherwise specified.

The antioxidant, anti-inflammatory and soothing repair composition comprises 16-24 parts by mass of cactus extract, preferably 16-20 parts by mass, and more preferably 16 parts by mass. In the invention, the cactus extract contains rich plant nutrients, can enhance the skin immunity, has obvious antioxidation and anti-infection effects, and has good effects of treating inflammation, whelk and the like on the skin; the cactus extract can promote cell regeneration and repair, restore skin elasticity, and protect skin.

Based on the mass parts of the cactus extract, the antioxidant, anti-inflammatory and soothing repair composition provided by the invention comprises 3.6-6 parts of licorice extract, preferably 3.6-4.8 parts, and more preferably 3.6 parts. In the invention, the licorice extract is white powder, has good water solubility, and is safe and nontoxic. Has antiinflammatory, antiulcer, antioxidant, antibacterial, wound healing, free radical scavenging, and epithelial tissue regeneration promoting effects.

Based on the mass parts of the cactus extract, the antioxidant, anti-inflammatory and soothing repair composition provided by the invention comprises 0.96-1.6 parts of nymphaea hybrid extract, preferably 1.28-1.6 parts, and more preferably 1.6 parts. In the invention, the nymphaea hybrid extract is rich in nutritional ingredients such as protein, carbohydrate, vitamins, active polypeptide, mineral substances, cellulose and the like required by a human body, and meanwhile, the plant placental extract contained in the nymphaea hybrid extract plays a very key role in balancing the endocrine system of the human body, can provide skin nutrients and mineral substances, can improve skin darkness after long-term use, enables the skin to be smooth, fine and glossy, and has remarkable effects of resisting oxidation, resisting wrinkles, replenishing water, brightening the skin and the like.

On the basis of the mass parts of the cactus extract, the antioxidant, anti-inflammatory and soothing repair composition provided by the invention preferably comprises 0.8-2.4 parts of oat peptide, preferably 1.6-2.4 parts, and more preferably 2.4 parts. In the invention, the oat peptide is a peptide existing in oat, and the common oat peptide has leupeptin, can strongly inhibit protease activity and has an antioxidant effect; meanwhile, the skin care product also has the function of permeation and helps the skin to better absorb other nutrient substances; meanwhile, the skin is provided with nutrition, the skin is conditioned, the moisture is effectively preserved, and the skin roughness is reduced. Are used mainly in cosmetics as antioxidants and skin conditioners.

The antioxidant, anti-inflammatory and soothing repair composition provided by the invention preferably comprises 0.8 part of rosmarinic acid based on the mass parts of the cactus extract. In the present invention, the rosmarinic acid has a very strong activity of scavenging free radicals in vivo and an anti-oxidation effect. The action mechanism is as follows: rosmarinic acid and unsaturated fatty acid are competitively combined with lipid peroxy to terminate the chain reaction of lipid peroxidation and reduce the lipid peroxidation rate; rosmarinic acid can inhibit release of lysosomes by reducing intracellular calcium ion concentration; inhibiting endothelial cell-mediated oxidation of low density lipoproteins.

The antioxidant, anti-inflammatory and soothing repair composition provided by the invention preferably comprises 0.8 part of trehalose based on the mass part of the cactus extract. In the invention, the trehalose has a stabilizing effect on the antioxidant polyphenol and has good compatibility, compatibility and stability with the antioxidant polyphenol. Trehalose can prevent protein denaturation, and maintain the effects of various proteins and polypeptide active ingredients; also has various biological functions, such as moisture retention, antioxidant activity, antibacterial activity, etc. Meanwhile, the trehalose can form a special protective film on the surface layer of the skin, so that the original nutrition and moisture of the skin are kept, the cell membrane structure is protected, and cells are activated; and the function of radiating external heat out can avoid sunburn and melanin deposition of the skin and effectively prevent the skin aging phenomenon.

The invention also provides a preparation method of the antioxidant, anti-inflammatory and soothing repair composition, which comprises the following steps: respectively soaking radix et caulis Opuntiae Dillenii, Glycyrrhrizae radix and herba Hylothelospermi Japonici in sequence, ultrasonic extracting to obtain supernatant, concentrating, and lyophilizing to obtain radix et caulis Opuntiae Dillenii extract, Glycyrrhrizae radix extract and herba Hylothelospermi flower extract; mixing the cactus extract, the licorice extract and the nymphaea hybrid extract to obtain the antioxidant, anti-allergy and soothing repair composition.

The invention sequentially soaks and ultrasonically extracts the cactus to obtain supernatant. The invention preferably uses cactus powder for soaking; the cactus powder is obtained by preferably crushing and sieving the cactus raw material. In the invention, the mesh number of the cactus powder is preferably 60-80 meshes, and more preferably 80 meshes.

In the present invention, the soaking agent is preferably distilled water; the mass volume ratio of the cactus powder to the distilled water is preferably 1 g: 25-30 mL, more preferably 1 g: 25 mL. In the invention, the soaking time is preferably 1-2 h, and more preferably 1 h. In the invention, the power of ultrasonic extraction is preferably 350-400W, and more preferably 400W; the temperature is preferably 30-35 ℃, and more preferably 30 ℃; the time is preferably 40-50 min, and more preferably 45 min. The supernatant is preferably obtained by centrifugation after ultrasonic extraction, and the centrifugation conditions are preferably at 4 ℃, 8000-10000 r/min, 10-15 min, more preferably at 4 ℃, 10000r/min, and 15 min. The invention fully extracts the effective substances by regulating and controlling the water consumption for soaking and the ultrasonic condition, thereby obtaining the cactus extract which is more beneficial to enhancing the skin immunity, resisting oxidation and resisting infection. The ultrasonic extraction and centrifugation equipment is not particularly limited, and the conventional ultrasonic extraction and centrifugation equipment in the field can be adopted.

After obtaining the supernatant, the invention concentrates the supernatant and freezes to obtain the cactus extract. In the present invention, the cactus extract is preferably in the form of powder. The invention is preferably concentrated to 1/3-1/4 of the volume of the original supernatant, and is more preferably concentrated to 1/4 of the original volume. After the concentrated solution is obtained, the concentrated solution is preferably stored at the temperature of-4 ℃ for 24 hours and then freeze-dried. In the invention, the freeze-drying time is preferably 32-36 h, and more preferably 36 h. In the present invention, the concentration and lyophilization can ensure the stability of the sample and the quantification of the subsequent sample, and the concentration and lyophilization have the same function and are not repeated unless otherwise specified. The method and the equipment for concentration and freeze-drying are not particularly limited, and the method and the equipment for concentration and freeze-drying are conventional in the field.

The invention sequentially soaks and ultrasonically extracts the liquorice to obtain supernatant. The invention preferably uses licorice powder to soak; further preferably, the licorice root raw material is pulverized and sieved to obtain licorice root powder. In the present invention, the licorice powder preferably has a mesh size of 60 to 80 mesh, and more preferably 80 mesh.

In the present invention, the soaking agent is preferably distilled water; the mass-volume ratio of the licorice powder to distilled water is preferably 1 g: 10-15 mL, more preferably 1 g: 10 mL. In the invention, the soaking time is preferably 1-2 h, and more preferably 1 h. In the invention, the power of ultrasonic extraction is preferably 250-300W, and more preferably 300W; the temperature is preferably 40-45 ℃, and more preferably 45 ℃; the time is preferably 30-40 min, and more preferably 30 min; the supernatant is preferably obtained by centrifugation after ultrasonic extraction, and the centrifugation conditions are preferably at 4 ℃, 8000-10000 r/min, 10-15 min, more preferably at 4 ℃, 10000r/min, and 15 min. According to the invention, by regulating and controlling the water consumption for soaking and ultrasonic conditions, effective substances are fully extracted, and the liquorice extract which is more beneficial to resisting oxidation, treating wounds, effectively removing free radicals and promoting epithelial cell tissue regeneration is obtained. The ultrasonic extraction and centrifugation equipment is not particularly limited, and the conventional ultrasonic extraction and centrifugation equipment in the field can be adopted.

After obtaining the supernatant, the invention concentrates the supernatant and freezes the licorice extract. In the present invention, the licorice extract is preferably in the form of powder. The concentration is preferably 1/4-1/5 of the original volume, and is more preferably 1/5 of the original volume. The concentrated supernatant is preferably stored at-4 ℃ for 24h and then lyophilized. The freeze-drying time is preferably 32-36 h, and preferably 36 h. The method and the equipment for concentration and freeze-drying are not particularly limited, and the method and the equipment for concentration and freeze-drying are conventional in the field.

The nymphaea hybrid is sequentially soaked and ultrasonically extracted to obtain supernatant. The invention preferably uses the nymphaea hybrid powder for soaking; preferably, the raw material of the nymphaea hybrid is crushed and sieved to obtain the nymphaea hybrid powder. In the invention, the mesh number of the nymphaea hybrid powder is preferably 60-80 meshes, and more preferably 80 meshes.

In the present invention, the soaking agent is preferably distilled water; the mass volume ratio of the nymphaea hybrid powder to the distilled water is preferably 1 g: 30-35 mL, more preferably 1 g: 35 mL. In the invention, the soaking time is preferably 1-2 h, and more preferably 1 h. In the invention, the power of ultrasonic extraction is preferably 350-400W, and more preferably 400W; the temperature is preferably 50-55 ℃, and more preferably 50 ℃; the time is preferably 30-40 min, and more preferably 30 min; the supernatant is preferably obtained by centrifugation after ultrasonic extraction, and the centrifugation conditions are preferably at 4 ℃, 8000-10000 r/min, 10-15 min, more preferably at 4 ℃, 10000r/min, and 15 min. According to the method, the water consumption for soaking and ultrasonic conditions are regulated and controlled, so that effective substances are fully extracted, and the nymphaea hybrid extract with remarkable oxidation resistance, wrinkle resistance, water replenishing and skin brightening effects is obtained. The ultrasonic extraction and centrifugation equipment is not particularly limited, and the conventional ultrasonic extraction and centrifugation equipment in the field can be adopted.

After obtaining the supernatant, the method concentrates and freezes the supernatant to obtain the nymphaea hybrid extract. In the present invention, the nymphaea hybrid extract is preferably in a powder form. The concentration is preferably carried out to 1/3-1/4 of the original volume, and more preferably to 1/4 of the original supernatant volume. The concentrated supernatant is preferably stored at-4 ℃ for 24h and then lyophilized. The freeze-drying time is preferably 32-36 h, and more preferably 36 h. The method and the equipment for concentration and freeze-drying are not particularly limited, and the method and the equipment for concentration and freeze-drying are conventional in the field.

The obtained cactus extract, the licorice extract and the nymphaea hybrid extract are mixed to obtain the antioxidant, anti-allergy, soothing and repairing composition.

In the invention, when the antioxidant, anti-inflammatory and soothing repair composition further comprises oat peptide, rosmarinic acid and trehalose, the invention preferably obtains a supernatant by sequentially crushing oat grains, degreasing and carrying out ultrasonic extraction by using a Tris-HCl buffer solution; mixing the supernatant with trichloroacetic acid with the same volume, and standing to obtain a standing supernatant; and sequentially concentrating and freeze-drying the standing supernatant to obtain the oat peptide. The invention further preferably pulverizes oat seeds and screens the pulverized oat seeds to obtain oat seed powder. In the present invention, the mesh number of the sieve is preferably 60 to 80 mesh, and more preferably 80 mesh.

After oat seed powder is obtained, the oat seed powder is preferably mixed with an extraction solvent for degreasing, and Tris-HCl buffer solution is subjected to ultrasonic extraction to obtain a supernatant. In the invention, the mass-volume ratio of the oat seed powder to the extraction solvent is preferably 1 g: 10-15 mL, more preferably 1 g: 10 mL; the extraction solvent is preferably n-hexane. After degreasing, the invention preferably further comprises drying the degreasing solution before ultrasonic extraction. In the invention, the concentration of the Tris-HCl buffer solution is preferably 0.1mol/L, and the volume of the Tris-HCl buffer solution is preferably 20-22 mL, and more preferably 20 mL. In the invention, the power of ultrasonic extraction is preferably 350-400W, and more preferably 400W; the temperature is preferably 25-30 ℃, and more preferably 30 ℃; the time is preferably 35-40 min, and more preferably 40 min. The invention preferably centrifuges the ultrasonic extraction liquid to obtain the supernatant, wherein the centrifugation condition is preferably at 4 ℃, 8000-10000 r/min, and centrifugation for 10-15 min, more preferably at 4 ℃, 10000r/min, and centrifugation for 15 min. According to the invention, the supernatant is preferably mixed with trichloroacetic acid with the same volume and is kept stand to obtain a standing supernatant. The mass concentration of the trichloroacetic acid is preferably 8-15%, and more preferably 12%; the standing time is preferably 30-40 min, and more preferably 35 min. The invention preferably centrifuges the mixed standing solution to obtain a standing supernatant; the centrifugation condition is preferably at 4 ℃ and 8000-10000 r/min, and the centrifugation is 10-15 min, more preferably at 4 ℃ and 10000r/min, and the centrifugation is 15 min. The ultrasonic extraction and centrifugation equipment is not particularly limited, and the conventional ultrasonic extraction and centrifugation equipment in the field can be adopted.

After the standing supernatant is obtained, the standing supernatant is concentrated and freeze-dried to obtain the oat peptide. In the present invention, the oat peptide is preferably in a powder form. The concentration is preferably carried out to 1/3-1/4 of the original volume, and more preferably to 1/4 of the original supernatant volume. The concentrated supernatant is preferably stored at-4 ℃ for 24h and then lyophilized. The freeze-drying time is preferably 32-36 h, and more preferably 36 h. The method and the equipment for concentration and freeze-drying are not particularly limited, and the method and the equipment for concentration and freeze-drying are conventional in the field.

The obtained cactus extract, the licorice extract, the nymphaea hybrid extract, the oat peptide trehalose and the rosmarinic acid are preferably mixed to obtain the antioxidant, anti-allergy and soothing repair composition.

According to the invention, the antioxidant, anti-inflammatory and soothing repair composition is preferably mixed with conventional auxiliary materials in the field to prepare various cosmetics for soothing and anti-allergy, and the antioxidant, anti-inflammatory and soothing repair composition accounts for 0.05-20%, preferably 0.1-5%, and further preferably 1.0-3.0% of the total mass of the cosmetics. The cosmetic provided by the invention is clear in components, has multiple effects of resisting inflammation, repairing and the like besides obvious antioxidant effect, is safe, does not have adverse reaction and has wide application prospect. The sources of the cosmetic auxiliary materials are not particularly limited, and the cosmetic auxiliary materials can be prepared from conventional commercial products.

In order to further illustrate the present invention, the following detailed description of the technical solutions provided by the present invention is made with reference to the accompanying drawings and examples, but they should not be construed as limiting the scope of the present invention.

Example 1

The components and contents of the antioxidant, anti-allergy and soothing repair composition in this example are as follows (by mass): 20 parts of cactus extract, 3.6 parts of liquorice extract, 1.6 parts of oat peptide, 1.28 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

Preparing a composition according to the proportion:

preparation of cactus extract: pulverizing a raw material sample cactus, sieving with a 60-mesh sieve, adding 1g of cactus powder into a beaker filled with 25mL of distilled water, soaking for 1h, then carrying out ultrasonic treatment for 45min under the conditions of ultrasonic power of 400W and ultrasonic temperature of 30 ℃, centrifuging for 15min under the condition of 4 ℃ of 10000r/min, taking supernatant, concentrating again, concentrating to 1/4 of the original volume, putting into a refrigerator of-4 ℃ for 24h, taking out, then using a freeze dryer for 36h, and freeze-drying to obtain powder, thereby obtaining the cactus extract.

Preparation of a licorice extract: crushing a raw material sample, sieving with a 80-mesh sieve, adding 1g of licorice powder into a beaker filled with 10mL of distilled water, soaking for 1h, then carrying out ultrasonic treatment for 30min under the conditions of ultrasonic power of 300W and ultrasonic temperature of 45 ℃, centrifuging for 15min under the condition of 4 ℃ of 10000r/min, taking supernatant, concentrating again, concentrating to 1/5 with the original volume, putting into a refrigerator with the temperature of-4 ℃ for 24h, taking out, then using a freeze dryer for 36h, and freeze-drying to obtain powder, thus obtaining the licorice extract.

Preparing a nymphaea hybrid extract: crushing raw material samples of nymphaea hybrid, sieving with a 80-mesh sieve, adding 1g of nymphaea hybrid powder into a beaker filled with 35mL of distilled water, soaking for 1h, then carrying out ultrasonic extraction for 30min under the conditions of ultrasonic power of 400W and ultrasonic temperature of 50 ℃, centrifuging for 15min under the condition of 10000r/min at 4 ℃, taking supernatant, concentrating again to obtain 1/3-1/4 of the original volume, putting the supernatant into a refrigerator at-4 ℃ for 24h, taking out, then using a freeze dryer for 36h, and freeze-drying to obtain powder, thereby obtaining the nymphaea hybrid extract.

Preparation of oat peptide: weighing oat grains, crushing, sieving with an 80-mesh sieve, putting 1g of oat grain powder into a conical flask, and degreasing with 10mL of n-hexane. Drying, adding 20mL of 0.1mol/L Tris-HCl buffer solution, performing ultrasonic extraction for 40min under the conditions of ultrasonic power of 400W and ultrasonic temperature of 30 ℃, and centrifuging for 15min at 10000r/min at 4 ℃ to obtain supernatant. Adding trichloroacetic acid with the same volume as 12% into the supernatant, mixing, standing for 30-40 min, centrifuging at 4 ℃ of 8000-10000 r/min for 10-15 min, taking the supernatant, concentrating again, concentrating to 1/4 of the original volume of the supernatant, putting into a refrigerator with the temperature of-4 ℃ for 24h, taking out, using a freeze dryer for 36h, and freeze-drying to obtain powder, thus obtaining the oat peptide.

According to the proportion, 0.7858g of cactus extract, 0.141444g of liquorice extract, 0.062864g of oat peptide extract, 0.0502912g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the antioxidant, anti-allergy and soothing repair composition.

Example 2

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 16 parts of cactus extract, 3.6 parts of liquorice extract, 0.8 part of oat peptide, 0.96 part of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

The preparation method of the composition is the same as that of example 1, wherein 0.62864g of cactus extract, 0.141444g of licorice extract, 0.031432g of oat peptide extract, 0.0377184g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed according to the proportion.

Example 3

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 24 parts of cactus extract, 3.6 parts of licorice extract, 2.4 parts of oat peptide, 1.6 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.94296g of cactus extract, 0.141444g of liquorice extract, 0.094296g of oat peptide extract, 0.062864g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Example 4

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 20 parts of cactus extract, 4.8 parts of liquorice extract, 2.4 parts of oat peptide, 0.96 part of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.7858g of cactus extract, 0.188592g of liquorice extract, 0.094296g of oat peptide extract, 0.0377184g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Example 5

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 24 parts of cactus extract, 4.8 parts of licorice extract, 0.8 part of oat peptide, 1.28 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.94296g of cactus extract, 0.188592g of liquorice extract, 0.031432g of oat peptide extract, 0.0502912g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Example 6

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 16 parts of cactus extract, 4.8 parts of liquorice extract, 1.6 parts of oat peptide, 1.6 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.62864g of cactus extract, 0.188592g of liquorice extract, 0.062864g of oat peptide extract, 0.062864g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Example 7

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 24 parts of cactus extract, 6 parts of licorice extract, 1.6 parts of oat peptide, 0.96 part of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.94296g of cactus extract, 0.23574g of liquorice extract, 0.062864g of oat peptide extract, 0.0377184g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Example 8

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 16 parts of cactus extract, 6 parts of licorice extract, 2.4 parts of oat peptide, 1.28 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.62864g of cactus extract, 0.23574g of liquorice extract, 0.094256g of oat peptide extract, 0.0502912g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Example 9

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 20 parts of cactus extract, 6 parts of liquorice extract, 0.8 part of oat peptide, 1.6 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.7858g of cactus extract, 0.23574g of liquorice extract, 0.031432g of oat peptide extract, 0.062864g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Example 10

The components and amounts of the antioxidant, anti-allergy and soothing repair composition in this example are as follows: 16 parts of cactus extract, 3.6 parts of licorice extract, 2.4 parts of oat peptide, 1.6 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

According to the proportion, 0.62864g of cactus extract, 0.141444g of liquorice extract, 0.094296g of oat peptide extract, 0.062864g of nymphaea hybrid extract, 0.031432g of trehalose and 0.031432g of rosmarinic acid are mixed to prepare the composition, and the preparation method is the same as that of example 1.

Comparative example 1

The only difference between comparative example 1 and example 10 is that in comparative example 1 the cactus extract is replaced by the same parts of distilled water, the total parts by weight of which are the same as in example 10.

Comparative example 2

The only difference between comparative example 1 and example 10 is that in comparative example 1, the licorice extract was replaced with the same parts of distilled water, and the total parts of the same were the same as in example 10.

Comparative example 3

The only difference between comparative example 1 and example 10 is that in comparative example 1, the nymphaea hybrid extract is replaced with the same parts of distilled water, and the total parts by weight thereof are the same as in example 10.

In order to verify the efficacy of the antioxidant, anti-inflammatory and soothing repair composition, the composition is detected by a free radical scavenging experiment, a hyaluronidase inhibition experiment and a cell repair activity efficacy evaluation experiment respectively.

Experimental example 1

ABTS free radical scavenging experiments on the antioxidant, anti-inflammatory and soothing repair compositions obtained in examples 1-10 and comparative examples 1-3.

The determination method of ABTS free radical clearance rate comprises the following steps: mixing the ABTS solution with the concentration of 7mmol/L and the potassium persulfate solution with the concentration of 2.45mmol/L according to the volume ratio of 1:1 to prepare a cation working solution of the ABTS free radical, and keeping out of the sun for 12-14 hours to excite the ABTS free radical. ABTS free radicals are diluted by PBS until the light absorption value is 0.7 +/-0.02 to prepare ABTS working solution.

Respectively preparing samples into solutions with three concentrations of 9.9mg/mL (1%), 29.7mg/mL (3%) and 49.5mg/mL (5%), respectively taking 0.09mL of the solution with each concentration, respectively adding 0.11mLABTS working solution, uniformly mixing, carrying out reaction at 37 ℃ in a dark place for 10min, and measuring an absorbance A at 734 nm; 0.09mL of each of the above-mentioned concentration solutions was taken, 0.11mL of PBS was added, and after the reaction, the absorbance A was measured at 734nm_iThen respectively reacting with 0.11mL of LaBTS working solution and 0.09mL of deionized water, and measuring the absorbance A at 734nm₀As a reference. The clearance calculation formula is:

EABTS＝[1-(A_i-A))/A₀]×100％

in the formula: EABTS is the clearance rate of ABTS free radicals,%;

a is the light absorption value of 0.09mL of sample solution added with 0.11mL of ABTS working solution;

A_iadding 0.11mL LPBS light absorption value for 0.09mL sample solution;

A₀absorbance of 0.09mL deionized water was added for 0.11mL of ABTS solution.

The results are shown in Table 1, as an average of three replicates.

TABLE 1 ABTS radical scavenging ratio for compositions of different concentrations in examples 1-10 and comparative examples 1-3

Experimental example 2

Anti-inflammatory and soothing repair compositions obtained in examples 1-10 and comparative examples 1-3 have hyaluronidase inhibition

1. The experimental principle is as follows: hyaluronidase is a major factor in causing allergic reactions. When the allergen invades the body for the first time, the body can generate antibody IgE, and when the allergen invades the body again, the allergen can be combined with IgE antibody on the surface of mast cells, so that an activation signal is started, histamine is released, and the histamine generates anaphylactic reaction under the activation action of hyaluronidase. Therefore, the activity of the hyaluronidase is inhibited, so that the anaphylactic reaction can be effectively prevented, and the hyaluronidase has strong correlation with inflammatory reaction and anaphylactic reaction. The antiallergic activity is based on the hyaluronidase inhibition rate, and the greater the hyaluronidase inhibition rate, the stronger the antiallergic activity.

2. The experimental steps are as follows:

2.1.1 preparation of acetic acid buffer solution (pH 5.6):

(1) 572 mu L of glacial acetic acid is weighed and diluted to 100mL by ultrapure water, and 4.8mL is taken as A solution after uniform mixing.

(2) 0.82g of sodium acetate crystal is weighed and dissolved in ultrapure water, the volume is adjusted to 100mL by the ultrapure water, and 45.2mL is taken as a B solution after uniform mixing.

(3) The solution A, B was mixed, and the volume was adjusted to 100mL with ultrapure water, and the mixture was mixed. Precisely measuring pH, and adjusting to 5.6 with solution A or B.

2.1.2 preparation of hyaluronidase solution:

0.01g of hyaluronidase was weighed into a beaker, and dissolved by adding 4mL of acetic acid buffer solution.

2.1.3 preparation of sodium hyaluronate solution (0.5 mg/mL):

0.01g of sodium hyaluronate was weighed and placed in a beaker, and 20mL of acetic acid buffer solution was added to dissolve the sodium hyaluronate.

2.1.4 preparation of Ellisib reagent (Ehrlichagent):

0.8g of p-dimethylaminobenzaldehyde is weighed and dissolved in 15mL of concentrated hydrochloric acid and 15mL of absolute ethyl alcohol, and the mixture can be stored for two months.

2.1.5 preparing sodium carbonate solution (1.0 mol/L):

5.3g of sodium carbonate was weighed, dissolved with ultrapure water and brought to a volume of 50 mL.

2.1.6 preparation of acetylacetone solution:

3.5mL of acetylacetone is dissolved in 50mL of 1.0mol/L sodium carbonate solution and prepared as before.

2.1.7 preparation of calcium chloride solution (0.25 mmol/L):

0.13873g of calcium chloride were weighed, dissolved in ultrapure water and made to volume of 100 mL.

2.1.8 preparing sodium hydroxide solution (0.4 mol/L):

1.6g of calcium chloride was weighed, dissolved with ultrapure water and made to volume of 100 mL.

2.2 assay of hyaluronidase Activity inhibition:

selecting the antioxidant, anti-inflammatory and soothing repair composition obtained in examples 1-10 and comparative examples 1-3 as a sample, and performing the following operations to obtain a light absorption value of a sample group:

(1) 0.1mL of 0.25mmol/L calcium chloride solution and 0.5mL of hyaluronidase solution are taken to be incubated at 37 ℃ for 20 min;

(2) adding 0.5mL of the sample solution prepared in the experimental example 1, and continuing to culture at 37 ℃ for 20 min;

(3) adding 0.5mL sodium hyaluronate solution, incubating at 37 deg.C for 30min, and standing at room temperature for 5 min;

(4) adding 0.1mL of 0.4mol/L sodium hydroxide solution and 0.5mL of acetylacetone solution, heating in a boiling water bath for 15min, and immediately cooling with ice water for 5 min;

(5) adding 1.0mL of an Ellisib reagent, diluting with 3.0mL of absolute ethyl alcohol, standing at normal temperature for 20min for color development, and measuring a light absorption value by using a spectrophotometer;

3. analysis of results

Hyaluronidase inhibition (%) - (A-B) - (C-D) ]/(A-B) × 100%

In the formula: a is the light absorption value of a blank group, and the blank group is that acetic acid buffer solution is used for replacing sample liquid;

b is the light absorption value of a blank control group, and the blank control group is that acetic acid buffer solution is used for replacing sample solution and hyaluronidase solution;

c is the light absorption value of the sample group;

d is the light absorption value of the sample control group, and the sample control group is formed by replacing the hyaluronidase solution with acetic acid buffer solution.

The results are shown in Table 2, as averaged in triplicate.

TABLE 2 inhibition of hyaluronidase by compositions of different concentrations in examples 1 to 10 and comparative examples 1 to 3

Experimental example 3

Evaluation of efficacy of anti-inflammatory, soothing repair compositions obtained in examples 1-10 and comparative examples 1-3 on skin repair Activity

1. Cytotoxicity study of composition on human immortalized epidermal cells (HaCaT) (CCK-8 method) the human immortalized epidermal cells (HaCaT) were purchased from shanghai cell bank of chinese academy of sciences.

1.1 cell Resuscitation

HaCaT cells were removed from liquid nitrogen and rapidly thawed in a 37 ℃ water bath to prevent the formation of ice crystals that would damage the cells. Centrifuging at 3000r/min at room temperature for 5min, discarding supernatant, adding l mL DMEM (Gibco, manufacturer) culture medium, pumping to mix well (containing 10% fetal calf serum and 1% double antibody) to obtain cell suspension, transferring the cell suspension to 25cm²Cell culture flask, adding 10mL DMEM medium, at 37 deg.C, 5% CO₂Culturing in an incubator with saturated humidity. The next day the medium was discarded and after gentle washing with sterile PBS, fresh DMEM medium was changed.

1.2 cell passages

When the cells grow to a logarithmic growth phase, removing the culture medium, washing with PBS 3 times, adding l mL of pancreatin, placing in an incubator for 3min, observing under an inverted microscope, if the cell gap becomes larger, rounding the cells, adding 2mL of the culture medium to terminate digestion,the cell suspension was aspirated into a 15mL centrifuge tube. Centrifuging at room temperature for 5min at 3000r/min, discarding supernatant, adding 3mL complete culture medium, blowing, mixing, and dividing to 25cm²In cell culture flasks, 10mL of DMEM medium was added to each flask at 37 deg.C with 5% CO₂And (5) continuously culturing in an incubator with saturated humidity.

1.3 cell count

After cells are digested by pancreatin, the mixture is centrifuged at 3000r/min for 5min at room temperature, the supernatant is discarded, 3mL of complete culture medium is added and blown to be mixed uniformly, 600 muL of cell suspension is sucked and added into a cell counting tube, the cell counting tube is placed under a cell counter to count, and the result is obtained, wherein the unit is one/mL (the average value is obtained by repeating twice).

1.4 Experimental procedure (CCK-8 method)

The CCK-8 reagent contains WST-8, which is reduced to yellow formazan dye with high water solubility by dehydrogenase in cell mitochondria under the action of an electron carrier (1-Methoxy PMS), and the generated formazan is proportional to the number of living cells, so that the reagent can be used for detecting cell proliferation.

(1) HaCaT cells were divided by 10 x 10⁴cells/mL, 100. mu.L/well, were plated in 96-well plates and incubated in an incubator, and different concentrations (as shown in Table 3) of the test compositions were added to incubate for 24h, 48h, until the cell density was about 80%.

(2) CCK-8 was diluted with the culture medium at a ratio of 1:9, and the liquid in a 96-well plate was aspirated, to which I00. mu.L of the diluted CCK-8 solution was added per well.

(3) Incubate in incubator for 30min, take out, detect absorbance value (OD) of each well at 450nm with microplate reader, this experiment is repeated more than three times, carry out statistical analysis difference between each group.

(4) Cell survival (%) ═ model group or sample group OD/normal group OD × 100%.

1.5 Experimental results and analysis

The cytotoxicity of the compositions of examples 1 to 10 and comparative examples 1 to 3 on HaCaT was examined by the CCK-8 method, and the results of example 10 are shown in Table 3, wherein Ctrl is cells of HaCaT cultured in a medium without adding the composition of the present invention. As can be seen from Table 3 and FIG. 1, the effect of different concentrations of the composition on cell proliferation was examined 24h after treatment of HaCaT cells. The results are as follows: the composition has no obvious cytotoxicity to normal HaCaT cells within the mass concentration range of 9.90-30.69 mg/mL (1.0% -3.2%), and when the concentration of the composition reaches 33.66mg/mL (3.4%), the survival rate of the HaCaT cells is remarkably reduced (P <0.05), as can be seen from Table 3 and figure 2, the influence of the compositions with different concentrations on cell proliferation after the composition is treated for 48 hours is detected. The results are as follows: the composition has no obvious cytotoxicity to normal HaCaT cells within the mass concentration range of 9.90-29.70 mg/mL (1.0% -3.0%), and when the concentration of the composition reaches 30.69mg/mL (3.1%), the survival rate of the HaCaT cells is remarkably reduced (P <0.05), which indicates that the composition has certain irritation to the HaCaT cells and influences the survival rate of the HaCaT cells due to overhigh concentration. Therefore, the concentration range of the composition in subsequent experiments is controlled to be 9.90-29.70 mg/mL (1.0% -3.0%) and the influence of the compositions with different concentrations on cell proliferation after the HaCaT cells are treated for 24h and 48h is shown in Table 3

Concentration of composition (mg/mL)	Cell Activity (%) (24h)	Cell Activity (%) (48h)
			Ctrl	100±0.65	100±3.48
9.9(1.0％)	94.74±1.21	93±2.49
			13.86(1.4％)	95.43±2.50	95.07±2.79
17.82(1.8％)	96.75±5.51	96.48±2.19
			21.78(2.2％)	100.27±3.01	98.31±3.15
25.74(2.6％)	100.47±3.20	94.81±4.30
			27.72(2.8％)	100.75±3.50	92.51±2.44
29.70(3.0％)	98.47±2.80	91.07±2.32
			30.69(3.1％)	91.68±1.99	85.61±4.30
31.68(3.2％)	89.98±3.71	80.32±3.38
			33.66(3.4％)	80.79±2.64	71.09±2.94
34.65(3.5％)	73.86±3.05	67.48±4.31

2. Effect of the composition on HaCaT cell wound healing (cell scratch test)

2.1 experimental principle: the cell scratching method is a method for simply measuring cell migration movement and repair capacity, is similar to an in vitro wound healing model, removes cells at the central part on a monolayer adherent cell cultured on an in vitro culture dish or a flat plate, then continuously cultures the cells for a set time of an experiment, takes out a cell culture plate, observes whether peripheral cells grow (repair) to a central scratching area, and judges the growth migration capacity of the cells according to the result.

2.2 Experimental procedures:

sterilizing the desired material, including straightedges and marker pens

(1) And (4) uniformly marking transverse lines at the back of the 6-hole plate by using a ruler and a marker pen, and traversing through the holes at intervals of 0.5-1 cm. Each hole passes through at least 5 lines.

(2) About 50X 10 additions to the wells⁵And inoculating HaCaT cells, wherein the inoculation principle is that the fusion rate reaches 100% after overnight.

(3) On the next day, the gun head is used, compared with the straight ruler, the gun head is perpendicular to the transverse line scratch on the back as much as possible, and the gun head is not inclined.

(4) The cells are washed 3 times with PBS, the scratched cells are removed, and 2mL of the test compositions with different concentrations are added into the culture wells after the washing is finished and are marked.

(5) Adding 5% CO at 37 deg.C₂And (5) an incubator for culture. Samples can be taken at 12 and 24h time points and photographed.

(6) The width of the cell scratch on each photo was determined using Image pro-plus software according to the formula: the scratch healing rate (scratch width of 0 h-scratch width at the detection time point)/scratch width of 0h × 100%, and the rate of migration was reflected by the scratch healing rate.

2.3 results of the experiment

FIG. 3 shows the effect of the anti-inflammatory, soothing repair composition on the promotion of HaCaT migration. The influence of the compositions with different concentrations on the migration capacity of HaCaT cells is discussed by adopting a cell in-vitro scratch experiment. The effect of the composition on HaCaT cell migration is shown in Table 4, where the cell migration rate was increased at different time points after scratching compared to the control group at the composition concentrations of 9.90mg/mL (1.0%) and 29.70mg/mL (3.3%). The mobility of the 9.90mg/mL (1.0%) composition group reached 26.54 + -2.49% compared with the mobility of the control group at 12h after scratching, and the mobility of the 9.90mg/mL (1.0%) composition group reached 43.91 + -3.06% compared with the mobility of the control group at 24h after scratching, and was significantly different from the control group (P < 0.05). The mobility of the 29.7mg/mL (3.0%) composition group reached 59.07 + -4.02% compared with the control group mobility at 12h after scratching, and the mobility of the 29.7mg/mL (3.0%) composition group reached 73.92 + -3.68% compared with the control group mobility at 24h after scratching, and was significantly different (P <0.05) compared with the control group mobility at 24.81 + -1.95%. The above results show that the composition can promote migration of HaCaT cells at lower doses at concentrations of 9.90mg/mL (1.0%) and 29.70mg/mL (3.0%), and that the composition can significantly promote migration of HaCaT cells at doses at concentrations of 29.70mg/mL (3.3%).

TABLE 4 results of composition on HaCaT cell scratch healing Rate (%)

Composition comprising a metal oxide and a metal oxide	0h		12h		24h
						Control group control	0	11.39±2.93％	24.81±1.95％
9.90mg/mL(1.0％)	0	26.54±2.49％	43.91±3.06％
				29.7mg/mL(3.0％)	0	59.07±4.02％	73.92±3.68％

From Table 4, the effect of the anti-inflammatory, soothing repair composition on the promotion of HaCaT migration is evident. The influence of the compositions with different concentrations on the migration capacity of HaCaT cells is discussed by adopting a cell in-vitro scratch experiment. The effect of the composition on HaCaT cell migration is shown in fig. 3. The above results indicate that the composition is able to promote migration of HaCaT cells at lower doses at concentrations of 9.90mg/mL (1.0%) and 29.70mg/mL (3.0%), and that the composition is able to significantly promote migration of HaCaT cells at doses at concentrations of 29.70mg/mL (3.0%).

As can be seen from the above examples and experimental examples, the antioxidant, anti-inflammatory and soothing repair composition prepared by the present invention has: (1) oxidation resistance: the prepared composition has the clearance rate of ABTS free radicals of more than 90 percent and has good oxidation resistance; (2) resistance to sensitivity: the hyaluronic acid inhibition rate of the prepared composition is more than 80%, and the composition has the effects of improving the skin fineness, enhancing the skin resistance, relieving the allergic symptoms and refreshing the skin; (3) no irritation: through HaCaT cytotoxicity experiments, the concentration range of the prepared composition is controlled to be 9.90-29.70 mg/mL (1.0-3.0%), and the composition is nonirritating; (4) repairing property: the composition prepared by the invention can promote the migration of HaCaT cells when being added with lower doses of 9.90mg/mL (1.0%) and 29.70mg/mL (3.0%), and can remarkably promote the migration of HaCaT cells when being added with doses of 29.70mg/mL (3.0%), so that the composition has remarkable effects of relieving and repairing, improving the skin resistance and comprehensively conditioning the skin state.

Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.

Claims

1. An antioxidant, anti-inflammatory and soothing repair composition is characterized by comprising the following components in parts by weight: 16-24 parts of cactus extract, 3.6-6 parts of licorice extract and 0.96-1.6 parts of nymphaea hybrid extract.

2. The composition according to claim 1, further comprising the following components in parts by weight: 0.8-2.4 parts of oat peptide, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

3. The composition according to claim 2, characterized by comprising the following components in parts by weight: 16 parts of cactus extract, 3.6 parts of licorice extract, 2.4 parts of oat peptide, 1.6 parts of nymphaea hybrid extract, 0.8 part of rosmarinic acid and 0.8 part of trehalose.

4. A method for preparing an antioxidant, anti-inflammatory and soothing repair composition, comprising the steps of:

respectively soaking radix et caulis Opuntiae Dillenii, Glycyrrhrizae radix and herba Hylothelospermi Japonici in sequence, ultrasonic extracting to obtain supernatant, concentrating, and lyophilizing to obtain radix et caulis Opuntiae Dillenii extract, Glycyrrhrizae radix extract and herba Hylothelospermi flower extract;

5. The method of claim 4, wherein when the antioxidant, anti-inflammatory, and soothing repair composition further comprises avenin, rosmarinic acid, and trehalose, the method further comprises:

6. The production method according to claim 4 or 5, wherein the concentration includes:

concentrating the clear cactus palms, the clear nymphaea hybrid supernatant and the clear oat peptide supernatant to 1/3-1/4 corresponding to the original volume respectively;

7. The production method according to claim 5, wherein the degreasing solvent comprises n-hexane; the concentration of the Tris-HCl buffer solution is 0.1 mol/L; the mass percentage concentration of the trichloroacetic acid is 12 percent.

8. Use of the antioxidant, anti-inflammatory and soothing repair composition of any one of claims 1 to 3 for the preparation of a cosmetic product with antioxidant, anti-inflammatory and soothing repair functions.

9. Use according to claim 8, wherein the antioxidant, anti-inflammatory and soothing repair composition represents between 0.05% and 20% of the total mass of the cosmetic product.

10. Use according to claim 8, wherein the antioxidant, anti-inflammatory and soothing repair composition represents 1.0% to 3.0% of the total mass of the cosmetic product.