CN115501142B - Facial essence and preparation process thereof - Google Patents
Facial essence and preparation process thereof Download PDFInfo
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- CN115501142B CN115501142B CN202211175571.XA CN202211175571A CN115501142B CN 115501142 B CN115501142 B CN 115501142B CN 202211175571 A CN202211175571 A CN 202211175571A CN 115501142 B CN115501142 B CN 115501142B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to the technical field of cosmetics, in particular to a facial essence and a preparation process thereof, wherein the facial essence containing lucid ganoderma fermentation filtrate comprises the following raw materials in parts by weight: 40-60 parts of lucid ganoderma fermentation filtrate, 2-4 parts of rosa rugosa essential oil, 0.2-1 part of carnosine, 0.5-2 parts of allantoin, 0.5-2.5 parts of nicotinamide, 10-20 parts of sodium hyaluronate, 5-10 parts of glycerin and 20-30 parts of water; the preparation method of the ganoderma lucidum fermentation filtrate comprises the following steps: pulverizing Ganoderma, mixing with water, and sterilizing to obtain raw materials to be fermented; ganoderma fermentation filtrate is obtained by fermenting Ganoderma with various strains including Lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071, bacillus subtilis CMCC 63501 and yeast CICC 1252. According to the invention, the ganoderma lucidum is fermented by a specific strain, the content of effective skin care components in the obtained ganoderma lucidum fermentation filtrate is high, and the effects of moisture preservation, wrinkle resistance and the like of the essence are improved by combining the ganoderma lucidum fermentation filtrate with other skin care components such as the essential oil of the rosa rugosa.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to facial essence and a preparation process thereof.
Background
The essence is a common skin care product for the face, and generally has the effects of preventing aging, resisting wrinkle, preserving moisture, whitening, removing spots and the like. Some plant extract components are often added into the existing essence to strengthen the efficacy of the essence in repairing skin.
Ganoderma lucidum is a dried fruiting body of Polyporaceae fungus Ganoderma lucidum Ganoderma lucidum (Leys. Ex Fr.) Karst or Ganoderma sinensis Ganoderma sinense Zhao, xu et Zhang. Has effects of invigorating qi, tranquilizing mind, relieving cough and asthma. It is mainly used for treating restlessness, insomnia, palpitation, cough, asthma, excessive phlegm, and consumptive disease. The Ganoderma contains polysaccharide, nucleosides, furans, sterols, alkaloids, triterpenes, oils and fats, various amino acids, proteins, enzymes, organogermanium, various microelements, etc. Ganoderan has effects of regulating immunity, reducing blood sugar, reducing blood lipid, resisting oxidation, resisting aging and resisting tumor; triterpene compounds can purify blood and protect liver function; the Ganoderma preparation has effects of tranquilizing, relieving convulsion, tonifying heart, relieving arrhythmia, lowering blood pressure, relieving cough and asthma; in addition, ganoderma lucidum has the functions of anticoagulation, platelet aggregation inhibition and antiallergic effect.
In the prior art, many people use lucid ganoderma or fermented extracts thereof for preparing skin care products with various effects, but the inventor finds that the effects of the existing skin care products containing lucid ganoderma components on moisture preservation, skin repair and the like still need to be improved.
Disclosure of Invention
The invention aims to provide a facial essence and a preparation process thereof, wherein a specific strain is selected for fermenting lucid ganoderma, the content of effective skin care components in the obtained lucid ganoderma fermentation filtrate is high, and the lucid ganoderma fermentation filtrate is combined with other skin care components such as rose essential oil, so that the effects of moisturizing, anti-wrinkle, whitening and spot removing of the facial essence are improved; and the addition of the rosa rugosa essential oil enhances the antibacterial effect of the essence, and is beneficial to long-time preservation.
In order to achieve the above purpose, the present invention provides the following technical solutions:
in a first aspect of the present invention, a facial essence containing a ganoderma lucidum fermentation filtrate is provided, which comprises the following raw materials in parts by weight:
40-60 parts of lucid ganoderma fermentation filtrate, 2-4 parts of rosa rugosa essential oil, 0.2-1 part of carnosine, 0.5-2 parts of allantoin, 0.5-2.5 parts of nicotinamide, 10-20 parts of sodium hyaluronate, 5-10 parts of glycerin and 20-30 parts of water;
the preparation method of the ganoderma lucidum fermentation filtrate comprises the following steps:
(1) Pulverizing Ganoderma, mixing with water, and sterilizing to obtain raw materials to be fermented;
(2) Respectively activating strains to obtain activated strains, respectively culturing the activated strains, and mixing to obtain mixed bacterial liquid; wherein the plurality of strains comprises lactobacillus reuteri DSM17938, bifidobacterium bifidum cic 6071, bacillus subtilis CMCC 63501, and yeast cic 1252;
(3) Inoculating the mixed bacterial liquid obtained in the step (2) into the raw material to be fermented obtained in the step (1), and fermenting for 24-48 hours at the temperature of 30-40 ℃ to obtain a fermentation product;
(4) Sterilizing the fermentation product to inactivate bacteria and obtain sterilized fermentation product, centrifuging the sterilized fermentation product, discarding precipitate, and collecting supernatant to obtain Ganoderma fermentation filtrate.
In one or more embodiments, the bacteria are inactivated in step (4) by autoclaving at 115 ℃ for 20 min;
in one or more embodiments, the inoculation amount of the mixed bacterial liquid in the step (3) is 1-3% of the mass percentage of the mixed bacterial liquid in the raw material to be fermented;
in one or more embodiments, the volume ratio of lactobacillus reuteri DSM17938, bifidobacterium bifidum cic 6071, bacillus subtilis CMCC 63501 and yeast cic 1252 seed solution in the mixed bacterial liquid in step (3) is 1:1-2:2-3:2-3;
in one or more embodiments, the method of preparing the palmarosa essential oil comprises the following steps:
(1) Adding a certain amount of NaCl aqueous solution into the rosa rugosa powder, wherein the mass volume ratio of the rosa rugosa powder to the NaCl aqueous solution is 1g:4-5mL; mixing, ultrasonic treating for a certain time, and distilling in boiling state;
(2) Standing for layering, removing water layer to obtain crude extract of herba Rosmarini officinalis essential oil, and adding anhydrous Na 2 SO 4 Absorbing water contained in the crude extract, sealing and preserving the dried crude extract, and standing to remove crystallized Na 2 SO 4 The obtained herba Rosmarini officinalis essential oil is preserved in dark.
In one or more embodiments, the NaCl solution in step (1) is a 4% by mass NaCl solution;
in one or more embodiments, the dried crude extract is hermetically stored at 4deg.C in step (2), and the crystallized Na is removed after standing for 22-24 hours 2 SO 4 ；
In one or more embodiments, the essential oil of the elliptica in step (2) is preserved at 0 to 4 ℃.
Preferably, the facial essence containing the lucid ganoderma fermentation filtrate comprises the following raw materials in parts by weight: 45-60 parts of lucid ganoderma fermentation filtrate, 2.5-4 parts of rosa rugosa essential oil, 0.3-1 part of carnosine, 0.6-2 parts of allantoin, 0.5-2 parts of nicotinamide, 12-20 parts of sodium hyaluronate, 6-10 parts of glycerin and 22-30 parts of water.
Further preferably, the facial essence containing the lucid ganoderma fermentation filtrate comprises the following raw materials in parts by weight: 45-55 parts of lucid ganoderma fermentation filtrate, 3-4 parts of rosa rugosa essential oil, 0.5-1 part of carnosine, 0.6-1.8 parts of allantoin, 0.5-1.5 parts of nicotinamide, 15-20 parts of sodium hyaluronate, 8-10 parts of glycerin and 25-30 parts of water.
The active ingredients such as triterpene compounds, polysaccharide and various amino acids contained in ganoderma lucidum can play roles in promoting cell proliferation and relieving oxidative stress injury of keratinocytes, can relieve the aging of epidermal cells, and the active ingredients can accelerate the regeneration of cells, increase the thickness of skin and increase collagen, so as to achieve the effects of plumping skin and eliminating fine lines and wrinkles. And ganoderan can also restore skin elasticity by maintaining and adjusting skin water, so that skin is moist and fine.
In the facial essence, the selected strains are used together to ferment lucid ganoderma, and the lucid ganoderma fermentation filtrate can obtain more substances such as triterpene compounds, polysaccharides, amino acids, polypeptides and the like.
In the facial essence, the rosa rugosa essential oil has the effects of moisturizing skin and regulating sebum secretion balance, and can promote epidermal cell regeneration and solve the problem of skin infection. Secondly, the rosa rugosa essential oil has a bactericidal function, and can relieve and treat acne, mild skin infection and skin inflammation. The essential oil of the rosa rugosa has good inhibition effect on bacteria and fungi, and especially can grow various moulds all the time. The prepared rosa rugosa essential oil is added into the facial essence, so that the rosa rugosa essential oil can play a role in bacteriostasis, can be stored for a long time on the premise of not adding a common cosmetic preservative, can also act together with polysaccharide components in the lucid ganoderma fermentation filtrate, can enhance the moisturizing and water locking functions of the essence, can reduce wrinkles on the skin surface, and can also relieve inflammation and telangiectasis on the skin surface.
The allantoin is added into the facial essence, and has the effects of sterilizing, preserving, relieving pain and resisting oxidation, so that the skin can keep moisture, moisten and soften, and the added allantoin has the effects of protecting tissues, hydrophilizing, absorbing water, preventing moisture from dispersing and the like; allantoin also promotes tissue growth, cellular metabolism, and softens stratum corneum proteins. The face essence is added with the nicotinamide which has the effect of resisting skin aging, and can alleviate and prevent the problem that skin is dull and yellow in the aging process; the invention has the advantages that the effect of repairing the stratum corneum can be achieved, the effect of water locking and moisture retention can be achieved, the proper amount of nicotinamide can achieve the good effect, but the opposite effect can be achieved when the amount of nicotinamide is increased. The facial essence is added with carnosine, and the carnosine has the antioxidation property and can play a role in preventing skin from oxidative aging by resisting free radicals in human bodies. In addition, the carnosine in the cosmetics can play roles in whitening skin and preventing skin aging, can maintain skin uniform complexion, improve skin self resistance and self-healing force, purify skin, maintain normal internal circulation of skin, accelerate discharge of skin metabolic products, maintain normal metabolism of skin and prevent skin aging. The allantoin, nicotinamide and carnosine act together and are combined with the ganoderma lucidum fermented extract, so that the facial essence has good skin repairing effect, can lighten skin and reduce textures.
The sodium hyaluronate and the glycerin in the facial essence are matched to serve as the humectant, so that the influence of the relative humidity of the surrounding environment on the moisture retention of the facial essence is smaller compared with other humectants.
In a second aspect of the present invention, a process for preparing the facial essence containing the ganoderma lucidum fermentation filtrate is provided, comprising the following steps:
(1) Weighing raw materials according to parts by weight;
(2) Mixing water, glycerol and sodium hyaluronate, heating for melting, and stirring uniformly;
(3) Cooling, adding Ganoderma fermentation filtrate, herba Rosmarini officinalis essential oil, carnosine, allantoin and nicotinamide, and mixing to obtain facial essence containing Ganoderma fermentation filtrate.
Compared with the prior art, the invention has the beneficial effects that:
the selected strains are matched for use to ferment lucid ganoderma, so that the lucid ganoderma fermentation filtrate can obtain more triterpene compounds, polysaccharides, amino acids, polypeptides and other substances, and the moisturizing and wrinkle removing effects of the skin of the facial essence containing the lucid ganoderma fermentation filtrate can be improved.
The prepared rosa rugosa essential oil is added into the facial essence, so that the rosa rugosa essential oil can play a role in bacteriostasis, can be stored for a long time on the premise of not adding a common cosmetic preservative, can also act together with polysaccharide components in the lucid ganoderma fermentation filtrate, can enhance the moisturizing and water locking functions of the essence, can reduce wrinkles on the skin surface, and can also relieve inflammation and telangiectasis on the skin surface.
The allantoin, nicotinamide and carnosine are added into the facial essence, and the components act together and are combined with the ganoderma lucidum fermented extract, so that the facial essence has good skin repairing effect, can lighten skin and reduce textures.
The sodium hyaluronate and the glycerin in the facial essence are matched to serve as the humectant, so that the influence of the relative humidity of the surrounding environment on the moisture retention of the facial essence is smaller compared with other humectants.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Preparing lucid ganoderma fermentation liquor:
(1) Pulverizing Ganoderma, mixing with water, and sterilizing to obtain raw materials to be fermented;
(2) Respectively activating strains to obtain activated strains, respectively culturing the activated strains, and mixing to obtain mixed bacterial liquid; wherein the plurality of strains comprise lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071, bacillus subtilis CMCC 63501 and saccharomycete CICC 1252, and the volume ratio of the seed solution of lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071, bacillus subtilis CMCC 63501 and saccharomycete CICC 1252 in the mixed bacterial solution is 1:1:2:2;
the method for activating the strain comprises the following steps: taking a small amount of strains, streaking on an MRS culture medium by adopting a parallel streaking method, and culturing for 20 hours at 37 ℃ to obtain an activated strain; wherein bifidobacterium bifidum cic 6071 is activated under anaerobic conditions. The composition of the MRS medium is: 10g of dried protein, 5g of beef powder, 4g of yeast powder, 2g of glucose, 1ml of tween 80, 2g of dipotassium hydrogen phosphate, 5g of sodium acetate, 2g of tri-ammonium citrate, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, 15g of agar powder and 1000ml of distilled water; adding the above components into distilled water, heating for dissolving, correcting pH to 6.2, packaging, and sterilizing at 121deg.C for 20 min.
The culture method of lactobacillus reuteri DSM17938, bacillus subtilis CMCC 63501 and saccharomycetes CICC 1252 seed liquid comprises the following steps: inoculating the activated bacterial solutions into liquid culture medium, and culturing at 37deg.C until the number of bacterial strains reaches 1×10 9 cfu/ml, respectively obtaining lactobacillus reuteri DSM17938, bacillus subtilis CMCC 63501 and saccharomycetes CICC 1252 seed solutions.
The culturing method of the bifidobacterium bifidum CICC 6071 comprises the following steps: firstly performing oxygen-resistant training, then inoculating the trained activated bacterial liquid into a liquid culture medium, and culturing at 37 ℃ until the number of strains reaches 1 multiplied by 10 9 cfu/ml to obtain bifidobacterium bifidum CICC 6071 seed solution.
(3) Inoculating the mixed bacterial liquid obtained in the step (2) into the raw material to be fermented obtained in the step (1), and fermenting at 30 ℃ for 24 hours to obtain a fermentation product;
(4) Sterilizing the fermentation product at 115 deg.C for 20min to inactivate bacteria and obtain sterilized fermentation product, centrifuging the sterilized fermentation product at 5000r/min for 25min, discarding precipitate, and collecting supernatant to obtain Ganoderma fermentation filtrate.
Extracting the essential oil of the herba rosae rugosae:
a certain amount of the herba rosae rugosae powder is put into a container, a certain amount of 4% NaCl aqueous solution is added, and the mass volume ratio of the herba rosae rugosae powder to the NaCl aqueous solution is 1g:5mL; uniformly mixing, performing ultrasonic treatment for a certain time, heating to a micro-boiling state, and extracting the essential oil of the rosa rugosa; standing for layering after extraction, removing water layer to obtain crude extract of herba Rosmarini officinalis essential oil, and using anhydrous Na 2 SO 4 Absorbing water contained in the crude extract, sealing and storing the dried crude extract at 4deg.C, standing for 24 hr, and removing crystallized Na 2 SO 4 Obtaining the essential oil of the rosa rugosa, placing the essential oil into a brown glass bottle for light-proof preservation and refrigerating at 0 ℃ for standby.
Preparation of facial essence containing ganoderma lucidum fermentation filtrate:
(1) Weighing the following raw materials in parts by weight: 45 parts of lucid ganoderma fermentation filtrate, 3 parts of rosa rugosa essential oil, 0.5 part of carnosine, 0.6 part of allantoin, 0.5 part of nicotinamide, 15 parts of sodium hyaluronate, 8 parts of glycerol and 25 parts of water.
(2) Mixing water, glycerol and sodium hyaluronate, heating for melting, and stirring uniformly;
(3) Cooling, adding Ganoderma fermentation filtrate, herba Rosmarini officinalis essential oil, carnosine, allantoin and nicotinamide, and mixing to obtain facial essence containing Ganoderma fermentation filtrate.
Example 2
Preparing lucid ganoderma fermentation liquor:
the differences from example 1 are: the volume ratio of the seed solution of lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071, bacillus subtilis CMCC 63501 and saccharomycete CICC 1252 in the mixed bacterial solution is 1:2:2:3; the remaining steps were the same as in example 1.
Extracting the essential oil of the herba rosae rugosae: this step is the same as in example 1.
Preparation of facial essence containing ganoderma lucidum fermentation filtrate:
(1) Weighing the following raw materials in parts by weight: 50 parts of lucid ganoderma fermentation filtrate, 3.5 parts of rosa rugosa essential oil, 0.6 part of carnosine, 0.8 part of allantoin, 1 part of nicotinamide, 16 parts of sodium hyaluronate, 9 parts of glycerol and 26 parts of water.
(2) Mixing water, glycerol and sodium hyaluronate, heating for melting, and stirring uniformly;
(3) Cooling, adding Ganoderma fermentation filtrate, herba Rosmarini officinalis essential oil, carnosine, allantoin and nicotinamide, and mixing to obtain facial essence containing Ganoderma fermentation filtrate.
Example 3
Preparing lucid ganoderma fermentation liquor:
the differences from example 1 are: the volume ratio of the seed solution of lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071, bacillus subtilis CMCC 63501 and saccharomycete CICC 1252 in the mixed bacterial solution is 1:1:2:3; the remaining steps were the same as in example 1.
Extracting the essential oil of the herba rosae rugosae: this step is the same as in example 1.
Preparation of facial essence containing ganoderma lucidum fermentation filtrate:
(1) Weighing the following raw materials in parts by weight: 52 parts of lucid ganoderma fermentation filtrate, 3.8 parts of rosa rugosa essential oil, 0.8 part of carnosine, 1 part of allantoin, 1.2 parts of nicotinamide, 18 parts of sodium hyaluronate, 9 parts of glycerol and 28 parts of water.
(2) Mixing water, glycerol and sodium hyaluronate, heating for melting, and stirring uniformly;
(3) Cooling, adding Ganoderma fermentation filtrate, herba Rosmarini officinalis essential oil, carnosine, allantoin and nicotinamide, and mixing to obtain facial essence containing Ganoderma fermentation filtrate.
Example 4
Preparing lucid ganoderma fermentation liquor:
the differences from example 1 are: the volume ratio of the seed solution of lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071, bacillus subtilis CMCC 63501 and saccharomycete CICC 1252 in the mixed bacterial solution is 1:1:3:3; the remaining steps were the same as in example 1.
Extracting the essential oil of the herba rosae rugosae: this step is the same as in example 1.
Preparation of facial essence containing ganoderma lucidum fermentation filtrate:
(1) Weighing the following raw materials in parts by weight: 55 parts of lucid ganoderma fermentation filtrate, 4 parts of rosa rugosa essential oil, 1 part of carnosine, 1.8 parts of allantoin, 1.5 parts of nicotinamide, 20 parts of sodium hyaluronate, 10 parts of glycerol and 30 parts of water.
(2) Mixing water, glycerol and sodium hyaluronate, heating for melting, and stirring uniformly;
(3) Cooling, adding Ganoderma fermentation filtrate, herba Rosmarini officinalis essential oil, carnosine, allantoin and nicotinamide, and mixing to obtain facial essence containing Ganoderma fermentation filtrate.
Comparative example 1: the facial essence containing the fermented ganoderma filtrate provided in this example is substantially the same as that in example 1, and the main difference is that: in the preparation of the ganoderma lucidum fermentation broth, a single strain lactobacillus reuteri DSM17938 is adopted.
Comparative example 2: the facial essence containing the fermented ganoderma filtrate provided in this example is substantially the same as that in example 1, and the main difference is that: in the preparation of the ganoderma lucidum fermentation liquid, the bacillus subtilis CMCC 63501 and the microzyme CICC 1252 are adopted in the volume ratio of 1: 1.
Comparative example 3: the facial essence containing the fermented ganoderma filtrate provided in this example is substantially the same as that in example 1, and the main difference is that: in the preparation of the ganoderma lucidum fermentation broth, lactobacillus reuteri DSM17938 and bifidobacterium bifidum CICC 6071 are adopted in a volume ratio of 1: 1.
Comparative example 4: the facial essence containing the fermented ganoderma filtrate provided in this example is substantially the same as that in example 1, and the main difference is that: in the preparation of the ganoderma lucidum fermentation broth, a mixed bacterial liquid of lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071 and bacillus subtilis CMCC 63501 with the volume ratio of 1:1:2 is adopted.
Comparative example 5: the facial essence containing the fermented ganoderma filtrate provided in this example is substantially the same as that in example 1, and the main difference is that: no carnosine, allantoin and nicotinamide were added.
Comparative example 6: the facial essence containing the fermented ganoderma filtrate provided in this example is substantially the same as that in example 1, and the main difference is that: no rosa rugosa essential oil was prepared and added.
Performance testing
The essences prepared in examples and comparative examples were subjected to a moisturizing effect test. The test method is as follows:
skin moisturization performance evaluation:
the facial essences of examples 1-4 and comparative examples 1-6 were tested for moisturizing properties by a human body method. The experimental conditions are as follows: the ambient temperature is 22-25 ℃, the humidity is 45-55%, and the real-time dynamic monitoring is carried out.
No cosmetic or topical drug can be used 3 days before the test site. Area markers were measured on both sides of the cleaned cheeks of the subject. The area of the test area is at least 3cm multiplied by 3cm, and a plurality of areas can be marked on the same cheek at the same time.
Evaluation method referring to QB/T4256-2011 cosmetic moisturizing efficacy evaluation guide
(1) The product smearing area and the blank control area should be randomly distributed in the left cheek calibration area and the right cheek calibration area, so that all the positions of the product and the blank area are ensured to reach equilibrium statistically.
(2) The test sample is coated for one time according to a certain dosage, the latex finger sleeve is used for uniformly coating the sample in a test area, and the actual sample coating amount is recorded.
(3) After adjusting the instrument according to the instructions of the skin analysis tester, measurements of the product area and the control area were performed, each area being measured in parallel at least 3 times. The initial values of each test area (before sample application) were measured and then after a set time the skin moisture content of the test and control areas was tested.
(4) Skin moisture content was measured for 10min, 30min, 1h after application, and 10min after washing, respectively, for the test and blank areas.
Testing of the same subject must be performed by the same measurement person using the same instrument, and the measurement probe should be cleaned between measurements.
The coating method comprises the following steps: the cheeks on both sides are correspondingly marked with circular areas with the diameter of 20mm, each area is separated by at least 1cm, different samples are respectively smeared on each area by 20 mu L, the smears are smeared once every 5min, after repeating for 4 times, the smears are used for testing the skin at the smears after 0h, 1h, 2h, 4h and 8 h.
TABLE 1
As can be seen from Table 1, the facial essence containing the fermented filtrate of Ganoderma lucidum prepared in examples 1-4 of the present invention has better moisturizing performance for 0-8 hours, and comparative examples 1-4 change the fermentation strain to ferment Ganoderma lucidum, and the obtained fermented liquid of Ganoderma lucidum has relatively less skin care active ingredients, so that the moisturizing effect is correspondingly reduced; the moisturizing effect of the facial extracts of comparative examples 5 and 6 was reduced relative to examples, but the reduction was not significant, without carnosine, allantoin, and nicotinamide added in comparative example 5, and without the addition of palmarosa essential oil in comparative example 6.
The facial essences prepared in examples 1 and 4 and comparative examples 1, 5 and 6 were subjected to skin repair efficacy test. The test method is as follows:
test instrument: VISIA-CR, test method: the subjects with wrinkles on the facial skin, selected for the age range of 30-50 years, were equally divided into 8 groups of 5 persons each. Skin wrinkle changes, including skin elasticity value, skin brightness and skin wrinkle condition, of the eyes were measured before facial use, after 14 days and after 28 days of using the essences described in examples 1 to 4 and comparative examples 1 to 4, respectively, and the results are shown in Table 2.
TABLE 2
As can be seen from table 2, the facial essence containing the fermented ganoderma filtrate prepared in examples 1 and 3 of the present invention has better skin restoration ability, and comparative example 1 changes the fermentation strain to ferment ganoderma, and the obtained fermented ganoderma liquid has relatively less skin care active ingredients, so that the skin restoration ability is correspondingly reduced; the skin repair ability of the facial lotions of comparative examples 5 and 6 was significantly reduced relative to examples 1 and 3 without carnosine, allantoin and nicotinamide added in comparative example 5 and without the rosa rugosa essential oil added in comparative example 6.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.
Claims (8)
1. A facial essence containing lucid ganoderma fermentation filtrate is characterized in that: the material comprises the following raw materials in parts by weight:
40-60 parts of lucid ganoderma fermentation filtrate, 2-4 parts of rosa rugosa essential oil, 0.2-1 part of carnosine, 0.5-2 parts of allantoin, 0.5-2.5 parts of nicotinamide, 10-20 parts of sodium hyaluronate, 5-10 parts of glycerin and 20-30 parts of water;
the preparation method of the ganoderma lucidum fermentation filtrate comprises the following steps:
(1) Pulverizing Ganoderma, mixing with water, and sterilizing to obtain raw materials to be fermented;
(2) Respectively activating strains to obtain activated strains, respectively culturing the activated strains, and mixing to obtain mixed bacterial liquid; wherein the plurality of strains comprises lactobacillus reuteri DSM17938, bifidobacterium bifidum cic 6071, bacillus subtilis CMCC 63501, and yeast cic 1252;
(3) Inoculating the mixed bacterial liquid obtained in the step (2) into the raw material to be fermented obtained in the step (1), and fermenting for 24-48 hours at the temperature of 30-40 ℃ to obtain a fermentation product; the volume ratio of the seed solution of lactobacillus reuteri DSM17938, bifidobacterium bifidum CICC 6071, bacillus subtilis CMCC 63501 and saccharomycete CICC 1252 in the mixed bacterial solution in the step (3) is 1:1-2:2-3:2-3;
(4) Sterilizing the fermentation product to inactivate bacteria and obtain sterilized fermentation product, centrifuging the sterilized fermentation product, discarding precipitate, and collecting supernatant to obtain Ganoderma fermentation filtrate;
the preparation method of the rosa rugosa essential oil comprises the following steps:
(1) Adding a certain amount of NaCl aqueous solution into the rosa rugosa powder, wherein the mass volume ratio of the rosa rugosa powder to the NaCl aqueous solution is 1g:4-5mL; mixing, ultrasonic treating for a certain time, and distilling in boiling state;
(2) Standing for layering, removing water layer to obtain crude extract of herba Rosmarini officinalis essential oil, and adding anhydrous Na 2 SO 4 Absorbing water contained in the crude extract, sealing and preserving the dried crude extract, and standing to remove crystallized Na 2 SO 4 The obtained herba Rosmarini officinalis essential oil is preserved in dark.
2. The facial concentrate containing a fermented filtrate of ganoderma lucidum according to claim 1, wherein: the inoculation amount of the mixed bacterial liquid in the step (3) is 1-3% of the mass percentage of the mixed bacterial liquid in the raw material to be fermented.
3. The facial concentrate containing a fermented filtrate of ganoderma lucidum according to claim 1, wherein: and (3) in the step (4), sterilizing at 115 ℃ for 20min, and inactivating bacteria.
4. The facial concentrate containing a fermented filtrate of ganoderma lucidum according to claim 1, wherein: in the preparation method of the rosa rugosa essential oil, the NaCl aqueous solution in the step (1) is 4% NaCl aqueous solution by mass percent.
5. The facial concentrate containing a fermented filtrate of ganoderma lucidum according to claim 1, wherein: the preparation method of the herba Rosmarini officinalis essential oil comprises the steps of (2) sealing and storing the dried crude extract at 4deg.C, standing for 22-24 hr, and removing crystallized Na 2 SO 4 The method comprises the steps of carrying out a first treatment on the surface of the And (3) preserving the essential oil of the rosa rugosa in the step (2) at the temperature of 0-4 ℃.
6. The facial concentrate containing a fermented filtrate of ganoderma lucidum according to claim 1, wherein: the face essence containing the lucid ganoderma fermentation filtrate comprises the following raw materials in parts by weight: 45-60 parts of lucid ganoderma fermentation filtrate, 2.5-4 parts of rosa rugosa essential oil, 0.3-1 part of carnosine, 0.6-2 parts of allantoin, 0.5-2 parts of nicotinamide, 12-20 parts of sodium hyaluronate, 6-10 parts of glycerin and 22-30 parts of water.
7. The facial concentrate containing a fermented filtrate of ganoderma lucidum according to claim 1, wherein: the face essence containing the lucid ganoderma fermentation filtrate comprises the following raw materials in parts by weight: 45-55 parts of lucid ganoderma fermentation filtrate, 3-4 parts of rosa rugosa essential oil, 0.5-1 part of carnosine, 0.6-1.8 parts of allantoin, 0.5-1.5 parts of nicotinamide, 15-20 parts of sodium hyaluronate, 8-10 parts of glycerin and 25-30 parts of water.
8. A process for preparing a facial concentrate containing a fermented filtrate of ganoderma lucidum according to any one of claims 1 to 7, characterized in that: the method comprises the following steps:
(1) Weighing raw materials according to parts by weight;
(2) Mixing water, glycerol and sodium hyaluronate, heating for melting, and stirring uniformly;
(3) Cooling, adding Ganoderma fermentation filtrate, herba Rosmarini officinalis essential oil, carnosine, allantoin and nicotinamide, and mixing to obtain facial essence containing Ganoderma fermentation filtrate.
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