CN105891387A - 一种用于检测亲水性氨基酸的富集方法 - Google Patents
一种用于检测亲水性氨基酸的富集方法 Download PDFInfo
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- CN105891387A CN105891387A CN201610320756.3A CN201610320756A CN105891387A CN 105891387 A CN105891387 A CN 105891387A CN 201610320756 A CN201610320756 A CN 201610320756A CN 105891387 A CN105891387 A CN 105891387A
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- amino acid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/08—Preparation using an enricher
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
Abstract
Description
时间(min) | 流速(mL/min) | 流动相A(%) | 流动相B(%) |
0 | 0.45 | 98 | 2 |
0.5 | 0.45 | 98 | 2 |
10 | 0.45 | 70 | 30 |
16 | 0.45 | 5 | 95 |
17 | 0.45 | 5 | 95 |
17.5 | 0.45 | 98 | 2 |
20 | 0.45 | 98 | 2 |
Claims (4)
Priority Applications (1)
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CN201610320756.3A CN105891387B (zh) | 2016-05-16 | 2016-05-16 | 一种用于检测亲水性氨基酸的富集方法 |
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CN201610320756.3A CN105891387B (zh) | 2016-05-16 | 2016-05-16 | 一种用于检测亲水性氨基酸的富集方法 |
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CN105891387A true CN105891387A (zh) | 2016-08-24 |
CN105891387B CN105891387B (zh) | 2018-01-19 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108854971A (zh) * | 2018-06-29 | 2018-11-23 | 中国人民解放军军事科学院军事医学研究院 | 一种兼性氮化碳亲水性富集材料及其制备方法与应用 |
CN109752465A (zh) * | 2017-11-01 | 2019-05-14 | 江苏维赛科技生物发展有限公司 | 一种采用高效液相色谱-质谱法测定乳粉中的牛磺酸含量的方法 |
CN110274973A (zh) * | 2019-07-16 | 2019-09-24 | 大连润生康泰医学检验实验室有限公司 | 一种血清中含硫氨基酸的检测方法及其富集材料 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103197022A (zh) * | 2013-04-25 | 2013-07-10 | 山西省食品质量安全监督检验研究所 | 一种检测食醋中所含氨基酸的方法 |
CN105548449A (zh) * | 2016-01-21 | 2016-05-04 | 同济大学 | 一种沼液中游离氨基酸的检测方法 |
-
2016
- 2016-05-16 CN CN201610320756.3A patent/CN105891387B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103197022A (zh) * | 2013-04-25 | 2013-07-10 | 山西省食品质量安全监督检验研究所 | 一种检测食醋中所含氨基酸的方法 |
CN105548449A (zh) * | 2016-01-21 | 2016-05-04 | 同济大学 | 一种沼液中游离氨基酸的检测方法 |
Non-Patent Citations (4)
Title |
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MICHAEL ARMSTRONG等: "Analysis of 25 underivatized amino acids in human plasma using ion-pairing reversed-phase liquid chromatography/time-of-flight mass spectrometry", 《RAPID COMMUNICATIONS IN MASS SPECTROMETRY》 * |
TOMAS HERRAIZ等: "Evaluation of solid-phase extraction procedures in peptide analysis", 《JOURNAL OF CHROMATOGRAPHY A》 * |
付志红等: "阴离子交换色谱-积分脉冲安培检测法测定寿胎丸中游离氨基酸", 《分析科学学报》 * |
周政华等: "固相萃取-离子色谱-积分脉冲安培法测定人血清中游离氨基酸", 《分析化学》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109752465A (zh) * | 2017-11-01 | 2019-05-14 | 江苏维赛科技生物发展有限公司 | 一种采用高效液相色谱-质谱法测定乳粉中的牛磺酸含量的方法 |
CN108854971A (zh) * | 2018-06-29 | 2018-11-23 | 中国人民解放军军事科学院军事医学研究院 | 一种兼性氮化碳亲水性富集材料及其制备方法与应用 |
CN110274973A (zh) * | 2019-07-16 | 2019-09-24 | 大连润生康泰医学检验实验室有限公司 | 一种血清中含硫氨基酸的检测方法及其富集材料 |
CN110274973B (zh) * | 2019-07-16 | 2021-03-26 | 大连润生康泰医学检验实验室有限公司 | 一种血清中含硫氨基酸的检测方法及其富集材料 |
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CN105891387B (zh) | 2018-01-19 |
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Inventor after: Bao Jimin Inventor after: Guo Zhimou Inventor after: Fan Xuran Inventor after: Dong Jun Inventor after: Meng Dawei Inventor after: Cao Yunfeng Inventor after: Sun Xiaoyu Inventor after: Fang Zhongze Inventor after: Ge Guangbo Inventor after: Hong Mo Inventor after: Gao Peng Inventor after: Liang Xinmiao Inventor before: Dong Jun Inventor before: Fan Xuran Inventor before: Cao Yunfeng Inventor before: Guo Zhimou Inventor before: Ge Guangbo Inventor before: Gao Peng Inventor before: Liang Xinmiao Inventor before: Fang Zhongze Inventor before: Sun Xiaoyu Inventor before: Hong Mo |
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Effective date of registration: 20180112 Address after: 121000 27 Fuzhou street, Taihe District, Liaoning, Jinzhou Co-patentee after: LIAONING PROVINCE JINQIU HOSPITAL Patentee after: LIAONING RUNSHENG KANGTAI BIOMEDICAL TECHNOLOGY CO., LTD. Address before: 121000 27 Fuzhou street, Taihe District, Liaoning, Jinzhou Patentee before: LIAONING RUNSHENG KANGTAI BIOMEDICAL TECHNOLOGY CO., LTD. |
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Effective date of registration: 20190802 Address after: 116000 Floor 3, 300-8 Jinlongsi Road, Ganjingzi District, Dalian City, Liaoning Province Co-patentee after: LIAONING PROVINCE JINQIU HOSPITAL Patentee after: Dalian Runsheng Kangtai Medical Laboratory Co., Ltd. Address before: 121000 27 Fuzhou street, Taihe District, Liaoning, Jinzhou Co-patentee before: LIAONING PROVINCE JINQIU HOSPITAL Patentee before: LIAONING RUNSHENG KANGTAI BIOMEDICAL TECHNOLOGY CO., LTD. |
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