CN105837700A - A comprehensive extraction, utilization and production method for glossy ganoderma sporocarps and spores - Google Patents

A comprehensive extraction, utilization and production method for glossy ganoderma sporocarps and spores Download PDF

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Publication number
CN105837700A
CN105837700A CN201610221431.XA CN201610221431A CN105837700A CN 105837700 A CN105837700 A CN 105837700A CN 201610221431 A CN201610221431 A CN 201610221431A CN 105837700 A CN105837700 A CN 105837700A
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ganoderma lucidum
take
extraction
controlling
temperature
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Inventor
毛日文
仲延龙
张莉
唐苏阳
沈井旺
周娟
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JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECHNOLOGY Co Ltd
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JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECHNOLOGY Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/04—Pretreatment of vegetable raw material
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/008—Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/16—Refining fats or fatty oils by mechanical means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

Abstract

The invention relates to a comprehensive extraction, utilization and production method for glossy ganoderma sporocarps and spores. The method includes (1) a step of extracting glossy ganoderma spore oil, namely a step of pretreating the glossy ganoderma spores, performing supercritical extraction, separating under reduced pressure, performing hyperfiltration and homogenizing to obtain a raffinate and the glossy ganoderma spore oil, and (2) a step of extracting glossy ganoderma poIysaccharides, namely a step of smashing the glossy ganoderma sporocarps, mixing with the raffinate, extracting, centrifuging, concentrating, precipitating with ethanol, performing enzymolysis and performing spray drying to obtain the glossy ganoderma poIysaccharides. The poIysaccharide extraction rate is increased by 25-32% than traditional processes. The glossy ganoderma poIysaccharides and the glossy ganoderma spore oil can be prepared into healthcare products, thus increasing production benefit and value.

Description

A kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method
Technical field
The present invention relates to spirit and glossy ganoderma deep process technology, particularly relate to a kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method.
Background technology
Glossy ganoderma master contains amino acid, polypeptide, protein, fungal lysozyme, and carbohydrate (reduced sugar and polysaccharide), ergosterol, triterpenes, coumarin glucoside, volatile oil, stearic acid, benzoic acid, alkaloid, vitamin B2 and C etc., it is Chinese medicine and the important source material of health food of state approval, typically it is difficult to reach therapeutic efficiency if lucid ganoderma fungus is used directly, need to carry out extracting, purifying, effect could be improved, play certain pharmacological action.
Ganoderma lucidum fruitbody is made up of cap and stem, glossy ganoderma in the growth and maturity phase time, it is hit by a bullet from glossy ganoderma lamella the most small avette reproduction cell i.e. Reishi sporule shot out, can be made into lucidum spore powder, for extracting extraction ganoderma lucidum spore oil, ganoderma lucidum spore oil Main Ingredients and Appearance is ganoderic acid, unrighted acid, GL-B etc., has a raising immunity of organisms, reduces the effect of the situations such as listless, the anorexia because Radiotherapy chemotherapy causes, vomiting, alopecia；
But its extract remainder still contains a large amount of polysaccharide, it is generally difficult to be utilized effectively, in the extraction process of prior art GL-B, using and ganoderma lucidum fruitbody is carried out conventional hot water's extraction or single super-pressure, enzyme process, there is complex technical process, extraction efficiency is low, the problems such as Polyose extraction is insufficient, extract the byproduct produced in the technique of polysaccharide simultaneously glossy ganoderma, be generally difficult to be fully used, affect process efficiency.
Summary of the invention
It is an object of the invention to the defect for prior art, it is provided that a kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, use overcritical C02Ganoderma lucidum spore oil in extracting ganoderma spore, its extract remainder again with jointly extract GL-B with ganoderma lucidum fruitbody, spore oil and polysaccharide extract rate are high, and ganoderma lucidum spore oil and GL-B can be used in healthy food material, thus improve glossy ganoderma productivity effect and value.
The present invention is achieved by the following technical solutions:
A kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, its method is as follows:
(1) ganoderma lucidum spore oil is extracted
1. pretreatment: take high-quality glossy ganoderma and scrape Reishi sporule; putting into microwave vacuum dryer, controlling baking temperature is 30 ~ 45 DEG C, carries out low temperature brittleness and is dried 10 ~ 15min; subsequently input air flow pulverizer carries out broken wall treatment, after broken wall, made the Reishi sporule particle of 20 ~ 40 mesh by dry-pressing granulator；
2. supercritical extract: take in the extraction kettle that Reishi sporule particle puts into equipped with ultrasonic oscillation device, be passed through supercritical CO2Open ultrasonic oscillation device simultaneously, control CO2Flow is 700 ~ 1000L/h, and extracting pressure is 35 ~ 45MPa, and extraction temperature is 40 ~ 50 DEG C, carries out supercritical extract 1 ~ 2h；
3. being depressured separation: the material after supercritical extract is put into separating still, controlling separating still temperature is 30 ~ 40 DEG C, and pressure is 10 ~ 15 MPa, collects a ganoderma lucidum spore oil now；
4. hyperfiltration: the material after separating step-down with the hyperfiltration film that molecular cut off is 50 ~ 100kD carries out hyperfiltration process, obtains secondary ganoderma lucidum spore oil and extract remainder；
5. homogeneous a: ganoderma lucidum spore oil and secondary ganoderma lucidum spore oil are put into homogenizer, controlling homogenizer pressure is 10 ~ 20MPa, and temperature is 30 ~ 40 DEG C, carries out homogeneous and processes 1 ~ 2 time, obtains ganoderma lucidum spore oil；
(2) GL-B is extracted
1. pulverize: the ganoderma lucidum fruitbody taking clean dried puts into pulverizer pulverizing, subsequently with 60 ~ 80 mesh sieve, obtains glossy ganoderma meal；
2. extraction: take glossy ganoderma meal and mix with extract remainder, adds the distilled water of volume 18 ~ 22 times amount, is 50 ~ 80W/cm in intensity2Under ultrasonic assistant, controlling temperature is 90 ~ 95 DEG C, carries out refluxing extraction 1 ~ 2h, and repeats refluxing extraction 3 times, collects and merges extract；
3. being centrifuged: take extract and put into centrifuge, controlling centrifugal rotational speed is 5000 ~ 8000 r/min, and centrifugal 10 ~ 15min takes supernatant；
4. concentrating: take supernatant and put into low-temperature vacuum thickener, controlling temperature is 20 ~ 30 DEG C, is concentrated into the 1/3 of original volume, obtains concentrate；
5. ethanol precipitation: take concentrate, by volume concentrate: 95% ethanol=1:3, adding ethanol mass concentration is the ethanol of 95%, staticly settling 5 ~ 8h, controlling centrifugal rotational speed subsequently is 4000 ~ 5000 r/min, centrifugal 5 ~ 10min, take precipitation, and precipitation is washed 1 ~ 2 time with acetone；
6. enzymolysis: take precipitation and add the distilled water of volume 2 ~ 3 times, add the complex enzyme of alkali protease, chymotrypsin and papain, controlling PH is 10 ~ 11, stir speed (S.S.) is 50 ~ 70r/min, carry out enzyme digestion reaction 2 ~ 3h, subsequently temperature is risen to 100 ~ 110 DEG C, and keep 5 ~ 7min, carry out enzyme-deactivating, prepare leaching liquor；
7. being spray-dried: take filtrate after being filtered by leaching liquor, put into spray dryer, the intake velocity controlling spray dryer is 40 ~ 80rpm, EAT is 150 ~ 180 DEG C, leaving air temp is 60 ~ 90 DEG C, and atomizer rotating speed is 12000 ~ 16000rpm, carries out being spray-dried prepared GL-B.
Above-mentioned a kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, wherein, the microwave frequency of described microwave vacuum dryer is 2450MHz ± 50Hz, and microwave output power is 10 ~ 20kW.
Above-mentioned a kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, wherein, the supersonic frequency of described ultrasonic oscillation device is 28 ~ 59KHz, and ultrasonic power is 120 ~ 600W.
Above-mentioned a kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, wherein, described complex enzyme neutral and alkali protease, chymotrypsin are 10:4:7 with the mass ratio of papain, and the interpolation quality of described complex enzyme is the 0.5 ~ 1% of precipitation quality.
Above-mentioned a kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, wherein, described ganoderma lucidum spore oil and GL-B, may use and prepare Ganoderma lucidum, its preparation method is as follows:
Softgel shell glue the most processed: take gelatin, G & W, after the 15 ~ 20min that at room temperature deaerates stirs, controlling temperature is 60 ~ 70 DEG C of insulation 40 ~ 50min, scrapes off liquid level foam, obtaining softgel shell glue, described gelatin, the weight ratio of G & W are 1:(0.4 ~ 0.6): 1；
Content the most processed: take 10 ~ 20 parts of ganoderma lucidum spore oils, 4 ~ 8 parts of GL-Bs, 3 ~ 4 parts of Radixs Astragali, 2 ~ 3 parts of rhodiola roots, 2 portions of Radix Glycyrrhizaes, 1 ~ 2 part of beeswax, 0.5 part of cassia seed, 0.1 ~ 0.2 portion of gynostemma pentaphylla, 0.1 ~ 0.2 portion of Chinese bulbul, 0.1 part of lutein and 0.01 ~ 0.05 part of vitaminAD according to the mass fraction, it is sequentially added in stirred tank, stir at 40 ~ 55 DEG C, cross colloid mill 2 ~ 3 times, deaerate and i.e. obtain content；
3. prepare Ganoderma lucidum: content and softgel shell glue put into and encapsulating machine is pressed into soft capsule, and the 3 ~ 4h that shapes at 20 ~ 25 DEG C, then wash ball, be dried, check, pack after i.e. obtain Ganoderma lucidum.
The invention have the benefit that
Microwave vacuum dryer of the present invention carries out brittle being dried, ultrasonic raising supercritical extract effect, and each process conditions are beneficial to improve working (machining) efficiency and shell-broken effect, and step-down separates and hyperfiltration is beneficial to be sufficiently separated ganoderma lucidum spore oil and extraction sky excess；With ultrasonic assistant hot water extraction, improving Polyose extraction effect, the protease in complex enzyme energy hydrolytic precipitation, rapid decomposing denatured protein, composite hydrolysis is effective；Ganoderma lucidum uses the ganoderma lucidum spore oil in Extraction method of ganoderan and GL-B, as content primary raw material, is aided with the new formulation such as the Radix Astragali, gynostemma pentaphylla, is beneficial to improve body immunity.
To sum up, the present invention makes full use of ganoderma lucidum fruitbody and the Reishi sporule of whole strain glossy ganoderma, the extract remainder of Reishi sporule, in order to jointly to extract polysaccharide with ganoderma lucidum fruitbody, polysaccharide extract rate is compared traditional handicraft and is improved 25 ~ 32%, ganoderma lucidum spore oil can produce health product with GL-B simultaneously, thus improves productivity effect and value.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described further.
Embodiment 1
A kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, its method is as follows:
(1) ganoderma lucidum spore oil is extracted
1. pretreatment: take high-quality glossy ganoderma and scrape Reishi sporule; put into microwave vacuum dryer; the microwave frequency of described microwave vacuum dryer is 2450MHz ± 50Hz; microwave output power is 10kW; controlling baking temperature is 35 DEG C; carry out low temperature brittleness and be dried 13min, subsequently input air flow pulverizer carries out broken wall treatment, after broken wall, made the Reishi sporule particle of 20 mesh by dry-pressing granulator；
2. supercritical extract: taking in the extraction kettle that Reishi sporule particle 2kg puts into equipped with ultrasonic oscillation device, the supersonic frequency of described ultrasonic oscillation device is 28KHz, and ultrasonic power is 120W, is passed through supercritical CO2Open ultrasonic oscillation device simultaneously, control CO2Flow is 800L/h, and extracting pressure is 42MPa, and extraction temperature is 45 DEG C, carries out supercritical extract 1.5h；
3. being depressured separation: the material after supercritical extract is put into separating still, controlling separating still temperature is 35 DEG C, and pressure is 13 MPa, collects a ganoderma lucidum spore oil now；
4. hyperfiltration: the material after separating step-down with the hyperfiltration film that molecular cut off is 50kD carries out hyperfiltration process, obtains secondary ganoderma lucidum spore oil and extract remainder 774g；
5. homogeneous a: ganoderma lucidum spore oil and secondary ganoderma lucidum spore oil are put into homogenizer, controlling homogenizer pressure is 10MPa, and temperature is 30 ~ 40 DEG C, carries out homogeneous and processes 1 ~ 2 time, obtains ganoderma lucidum spore oil 1105g；
(2) GL-B is extracted
1. pulverize: the ganoderma lucidum fruitbody 3kg taking clean dried puts into pulverizer, uses 60 mesh sieve subsequently, obtains glossy ganoderma meal；
2. extraction: take glossy ganoderma meal and mix with extract remainder, adds the distilled water of volume 21 times amount, is 60W/cm in intensity2Under ultrasonic assistant, controlling temperature is 92 DEG C, carries out refluxing extraction 1.5h, and repeats refluxing extraction 3 times, collects and merges extract；
3. being centrifuged: take extract and put into centrifuge, controlling centrifugal rotational speed is 6000 r/min, and centrifugal 13min takes supernatant；
4. concentrating: take supernatant and put into low-temperature vacuum thickener, controlling temperature is 24 DEG C, is concentrated into the 1/3 of original volume, obtains concentrate；
5. ethanol precipitation: take concentrate, by volume concentrate: 95% ethanol=1:3, adding ethanol mass concentration is the ethanol of 95%, staticly settles 7h, and controlling centrifugal rotational speed subsequently is 4000 r/min, centrifugal 7min, takes precipitation, and precipitation is washed 2 times with acetone；
6. enzymolysis: take precipitation and add the distilled water of volume 2.5 times, add the complex enzyme of alkali protease, chymotrypsin and papain, described complex enzyme neutral and alkali protease, chymotrypsin are 10:4:7 with the mass ratio of papain, and the interpolation quality of described complex enzyme is the 0.5% of precipitation quality, and controlling PH is 10.5, stir speed (S.S.) is 60r/min, carry out enzyme digestion reaction 2h, subsequently temperature is risen to 100 DEG C, and keep 5min, carry out enzyme-deactivating, prepare leaching liquor；
7. being spray-dried: take filtrate after being filtered by leaching liquor, put into spray dryer, the intake velocity controlling spray dryer is 60rpm, EAT is 150 DEG C, leaving air temp is 60 DEG C, and atomizer rotating speed is 12000rpm, carries out being spray-dried prepared GL-B 694g；
Described ganoderma lucidum spore oil and GL-B, may use and prepare Ganoderma lucidum, and its preparation method is as follows:
Softgel shell glue the most processed: take gelatin, G & W, after at room temperature degassing 18min stirs, controlling temperature is 60 DEG C of insulation 45min, scrapes off liquid level foam, obtains softgel shell glue, and described gelatin, the weight ratio of G & W are 1:(0.4 ~ 0.6): 1；
Content the most processed: take 18 parts of ganoderma lucidum spore oils, 7 parts of GL-Bs, 3 parts of Radixs Astragali, 2 parts of rhodiola roots, 2 portions of Radix Glycyrrhizaes, 1 part of beeswax, 0.5 part of cassia seed, 0.1 portion of gynostemma pentaphylla, 0.2 portion of Chinese bulbul, 0.1 part of lutein and 0.02 part of vitaminAD according to the mass fraction, it is sequentially added in stirred tank, stir at 45 DEG C, cross colloid mill 2 times, deaerate and i.e. obtain content；
3. prepare Ganoderma lucidum: content and softgel shell glue put into and encapsulating machine is pressed into soft capsule, and the 3.5h that shapes at 22 DEG C, then wash ball, be dried, check, pack after i.e. obtain Ganoderma lucidum.
Embodiment 2
A kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, its method is as follows:
(1) ganoderma lucidum spore oil is extracted
1. pretreatment: take high-quality glossy ganoderma and scrape Reishi sporule; put into microwave vacuum dryer; the microwave frequency of described microwave vacuum dryer is 2450MHz ± 50Hz; microwave output power is 20kW; controlling baking temperature is 35 DEG C; carry out low temperature brittleness and be dried 15min, subsequently input air flow pulverizer carries out broken wall treatment, after broken wall, made the Reishi sporule particle of 40 mesh by dry-pressing granulator；
2. supercritical extract: taking in the extraction kettle that Reishi sporule particle 3kg puts into equipped with ultrasonic oscillation device, the supersonic frequency of described ultrasonic oscillation device is 35KHz, and ultrasonic power is 400W, is passed through supercritical CO2Open ultrasonic oscillation device simultaneously, control CO2Flow is 1000L/h, and extracting pressure is 42MPa, and extraction temperature is 47 DEG C, carries out supercritical extract 2h；
3. being depressured separation: the material after supercritical extract is put into separating still, controlling separating still temperature is 31 DEG C, and pressure is 15 MPa, collects a ganoderma lucidum spore oil now；
4. hyperfiltration: the material after separating step-down with the hyperfiltration film that molecular cut off is 80kD carries out hyperfiltration process, obtains secondary ganoderma lucidum spore oil and extract remainder 1084g；
5. homogeneous a: ganoderma lucidum spore oil and secondary ganoderma lucidum spore oil are put into homogenizer, controlling homogenizer pressure is 14MPa, and temperature is 33 DEG C, carries out homogeneous and processes 2 times, obtains ganoderma lucidum spore oil 1855g；
(2) GL-B is extracted
1. pulverize: the ganoderma lucidum fruitbody 2kg taking clean dried puts into pulverizer, uses 60 mesh sieve subsequently, obtains glossy ganoderma meal；
2. extraction: take glossy ganoderma meal and mix with extract remainder, adds the distilled water of volume 18 times amount, is 50W/cm in intensity2Under ultrasonic assistant, controlling temperature is 95 DEG C, carries out refluxing extraction 1h, and repeats refluxing extraction 3 times, collects and merges extract；
3. being centrifuged: take zymotic fluid and put into centrifuge, controlling centrifugal rotational speed is 6000 r/min, and centrifugal 13min takes supernatant；
4. concentrating: take supernatant and put into low-temperature vacuum thickener, controlling temperature is 26 DEG C, is concentrated into the 1/3 of original volume, obtains concentrate；
5. ethanol precipitation: take concentrate, by volume concentrate: 95% ethanol=1:3, adding ethanol mass concentration is the ethanol of 95%, staticly settles 8h, and controlling centrifugal rotational speed subsequently is 5000 r/min, centrifugal 6min, takes precipitation, and precipitation is washed 2 times with acetone；
6. enzymolysis: take precipitation and add the distilled water of volume 3 times, add the complex enzyme of alkali protease, chymotrypsin and papain, described complex enzyme neutral and alkali protease, chymotrypsin are 10:4:7 with the mass ratio of papain, and the interpolation quality of described complex enzyme is the 1% of precipitation quality, and controlling PH is 11, stir speed (S.S.) is 70r/min, carry out enzyme digestion reaction 2h, subsequently temperature is risen to 105 DEG C, and keep 6min, carry out enzyme-deactivating, prepare leaching liquor；
7. being spray-dried: take filtrate after being filtered by leaching liquor, put into spray dryer, the intake velocity controlling spray dryer is 70rpm, EAT is 165 DEG C, leaving air temp is 90 DEG C, and atomizer rotating speed is 14000rpm, carries out being spray-dried prepared GL-B 386g；
Described ganoderma lucidum spore oil and GL-B, may use and prepare Ganoderma lucidum, and its preparation method is as follows:
Softgel shell glue the most processed: take gelatin, G & W, after at room temperature degassing 18min stirs, controlling temperature is 67 DEG C of insulation 42min, scrapes off liquid level foam, obtains softgel shell glue, and described gelatin, the weight ratio of G & W are 1:(0.4 ~ 0.6): 1；
Content the most processed: take 18 parts of ganoderma lucidum spore oils, 6 parts of GL-Bs, 3 parts of Radixs Astragali, 2 parts of rhodiola roots, 2 portions of Radix Glycyrrhizaes, 1 part of beeswax, 0.5 part of cassia seed, 0.2 portion of gynostemma pentaphylla, 0.15 portion of Chinese bulbul, 0.1 part of lutein and 0.04 part of vitaminAD according to the mass fraction, it is sequentially added in stirred tank, stir at 48 DEG C, cross colloid mill 3 times, deaerate and i.e. obtain content；
3. prepare Ganoderma lucidum: content and softgel shell glue put into and encapsulating machine is pressed into soft capsule, and the 3h that shapes at 25 DEG C, then wash ball, be dried, check, pack after i.e. obtain Ganoderma lucidum.
Embodiment 3
A kind of ganoderma lucidum fruitbody and Reishi sporule comprehensive extraction and application production method, its method is as follows:
(1) ganoderma lucidum spore oil is extracted
1. pretreatment: take high-quality glossy ganoderma and scrape Reishi sporule; put into microwave vacuum dryer; the microwave frequency of described microwave vacuum dryer is 2450MHz ± 50Hz; microwave output power is 18kW; controlling baking temperature is 38 DEG C; carry out low temperature brittleness and be dried 12min, subsequently input air flow pulverizer carries out broken wall treatment, after broken wall, made the Reishi sporule particle of 20 mesh by dry-pressing granulator；
2. supercritical extract: taking in the extraction kettle that Reishi sporule particle 4kg puts into equipped with ultrasonic oscillation device, the supersonic frequency of described ultrasonic oscillation device is 35KHz, and ultrasonic power is 500W, is passed through supercritical CO2Open ultrasonic oscillation device simultaneously, control CO2Flow is 900L/h, and extracting pressure is 36MPa, and extraction temperature is 48 DEG C, carries out supercritical extract 2h；
3. being depressured separation: the material after supercritical extract is put into separating still, controlling separating still temperature is 37 DEG C, and pressure is 13 MPa, collects a ganoderma lucidum spore oil now；
4. hyperfiltration: the material after separating step-down with the hyperfiltration film that molecular cut off is 90kD carries out hyperfiltration process, obtains secondary ganoderma lucidum spore oil and extract remainder 1321g；
5. homogeneous a: ganoderma lucidum spore oil and secondary ganoderma lucidum spore oil are put into homogenizer, controlling homogenizer pressure is 15MPa, and temperature is 30 DEG C, carries out homogeneous and processes 2 times, obtains ganoderma lucidum spore oil 2598g；
(2) GL-B is extracted
1. pulverize: the ganoderma lucidum fruitbody 4kg taking clean dried puts into pulverizer, uses 80 mesh sieve subsequently, obtains glossy ganoderma meal；
2. extraction: take glossy ganoderma meal and mix with extract remainder, adds the distilled water of volume 22 times amount, is 80W/cm in intensity2Under ultrasonic assistant, controlling temperature is 93 DEG C, carries out refluxing extraction 2h, and repeats refluxing extraction 3 times, collects and merges extract；
3. it is centrifuged: taking extract and put into centrifuge, control centrifugal rotational speed is 6000r/min, centrifugal 13min, takes supernatant；
4. concentrating: take supernatant and put into low-temperature vacuum thickener, controlling temperature is 27 DEG C, is concentrated into the 1/3 of original volume, obtains concentrate；
5. ethanol precipitation: take concentrate, by volume concentrate: 95% ethanol=1:3, adding ethanol mass concentration is the ethanol of 95%, staticly settles 6h, and controlling centrifugal rotational speed subsequently is 4500 r/min, centrifugal 8min, takes precipitation, and precipitation is washed 2 times with acetone；
6. enzymolysis: take precipitation and add the distilled water of volume 2 ~ 3 times, add the complex enzyme of alkali protease, chymotrypsin and papain, described complex enzyme neutral and alkali protease, chymotrypsin are 10:4:7 with the mass ratio of papain, and the interpolation quality of described complex enzyme is the 0.6% of precipitation quality, and controlling PH is 10, stir speed (S.S.) is 60r/min, carry out enzyme digestion reaction 2.5h, subsequently temperature is risen to 100 ~ 110 DEG C, and keep 5 ~ 7min, carry out enzyme-deactivating, prepare leaching liquor；
7. being spray-dried: take filtrate after being filtered by leaching liquor, put into spray dryer, the intake velocity controlling spray dryer is 70rpm, EAT is 175 DEG C, leaving air temp is 75 DEG C, and atomizer rotating speed is 15000rpm, carries out being spray-dried prepared GL-B 873g；
Described ganoderma lucidum spore oil and GL-B, may use and prepare Ganoderma lucidum, and its preparation method is as follows:
Softgel shell glue the most processed: take gelatin, G & W, after at room temperature degassing 18min stirs, controlling temperature is 65 DEG C of insulation 50min, scrapes off liquid level foam, obtains softgel shell glue, and described gelatin, the weight ratio of G & W are 1:(0.4 ~ 0.6): 1；
Content the most processed: take 19 parts of ganoderma lucidum spore oils, 5 parts of GL-Bs, 3 parts of Radixs Astragali, 2 parts of rhodiola roots, 2 portions of Radix Glycyrrhizaes, 2 parts of beeswaxs, 0.5 part of cassia seed, 0.15 portion of gynostemma pentaphylla, 0.15 portion of Chinese bulbul, 0.1 part of lutein and 0.04 part of vitaminAD according to the mass fraction, it is sequentially added in stirred tank, stir at 48 DEG C, cross colloid mill 3 times, deaerate and i.e. obtain content；
3. prepare Ganoderma lucidum: content and softgel shell glue put into and encapsulating machine is pressed into soft capsule, and the 3h that shapes at 22 DEG C, then wash ball, be dried, check, pack after i.e. obtain Ganoderma lucidum.
The invention have the benefit that
The GL-B recovery rate of the present invention is compared traditional handicraft and is improved 25 ~ 32%, takes Ganoderma lucidum of the present invention February continuously, and complexion is ruddy, and defecation is smooth and easy, and immunity improves.
The above; being only the present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto, any those familiar with the art is in the technical scope that the invention discloses; the change that can readily occur in or replacement, all should contain within the scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.

Claims (5)

1. ganoderma lucidum fruitbody and a Reishi sporule comprehensive extraction and application production method, is characterized by, its method is as follows:
(1) ganoderma lucidum spore oil is extracted
1. pretreatment: take high-quality glossy ganoderma and scrape Reishi sporule; putting into microwave vacuum dryer, controlling baking temperature is 30 ~ 45 DEG C, carries out low temperature brittleness and is dried 10 ~ 15min; subsequently input air flow pulverizer carries out broken wall treatment, after broken wall, made the Reishi sporule particle of 20 ~ 40 mesh by dry-pressing granulator；
2. supercritical extract: take in the extraction kettle that Reishi sporule particle puts into equipped with ultrasonic oscillation device, be passed through supercritical CO2Open ultrasonic oscillation device simultaneously, control CO2Flow is 700 ~ 1000L/h, and extracting pressure is 35 ~ 45MPa, and extraction temperature is 40 ~ 50 DEG C, carries out supercritical extract 1 ~ 2h；
3. being depressured separation: the material after supercritical extract is put into separating still, controlling separating still temperature is 30 ~ 40 DEG C, and pressure is 10 ~ 15 MPa, collects a ganoderma lucidum spore oil now；
4. hyperfiltration: the material after separating step-down with the hyperfiltration film that molecular cut off is 50 ~ 100kD carries out hyperfiltration process, obtains secondary ganoderma lucidum spore oil and extract remainder；
5. homogeneous a: ganoderma lucidum spore oil and secondary ganoderma lucidum spore oil are put into homogenizer, controlling homogenizer pressure is 10 ~ 20MPa, and temperature is 30 ~ 40 DEG C, carries out homogeneous and processes 1 ~ 2 time, obtains ganoderma lucidum spore oil；
(2) GL-B is extracted
1. pulverize: the ganoderma lucidum fruitbody taking clean dried puts into pulverizer pulverizing, subsequently with 60 ~ 80 mesh sieve, obtains glossy ganoderma meal；
2. extraction: take glossy ganoderma meal and mix with extract remainder, adds the distilled water of volume 18 ~ 22 times amount, is 50 ~ 80W/cm in intensity2Under ultrasonic assistant, controlling temperature is 90 ~ 95 DEG C, carries out refluxing extraction 1 ~ 2h, and repeats refluxing extraction 3 times, collects and merges extract；
3. being centrifuged: take extract and put into centrifuge, controlling centrifugal rotational speed is 5000 ~ 8000 R/min, centrifugal 10 ~ 15min, take supernatant；
4. concentrating: take supernatant and put into low-temperature vacuum thickener, controlling temperature is 20 ~ 30 DEG C, is concentrated into the 1/3 of original volume, obtains concentrate；
5. ethanol precipitation: take concentrate, by volume concentrate: 95% ethanol=1:3, adding ethanol mass concentration is the ethanol of 95%, staticly settling 5 ~ 8h, controlling centrifugal rotational speed subsequently is 4000 ~ 5000 r/min, centrifugal 5 ~ 10min, take precipitation, and precipitation is washed 1 ~ 2 time with acetone；
6. enzymolysis: take precipitation and add the distilled water of volume 2 ~ 3 times, add the complex enzyme of alkali protease, chymotrypsin and papain, controlling PH is 10 ~ 11, stir speed (S.S.) is 50 ~ 70r/min, carry out enzyme digestion reaction 2 ~ 3h, subsequently temperature is risen to 100 ~ 110 DEG C, and keep 5 ~ 7min, carry out enzyme-deactivating, prepare leaching liquor；
7. being spray-dried: take filtrate after being filtered by leaching liquor, put into spray dryer, the intake velocity controlling spray dryer is 40 ~ 80rpm, EAT is 150 ~ 180 DEG C, leaving air temp is 60 ~ 90 DEG C, and atomizer rotating speed is 12000 ~ 16000rpm, carries out being spray-dried prepared GL-B.
A kind of ganoderma lucidum fruitbody the most according to claim 1 and Reishi sporule comprehensive extraction and application production method, is characterized by, the microwave frequency of described microwave vacuum dryer is 2450MHz ± 50Hz, and microwave output power is 10 ~ 20kW.
A kind of ganoderma lucidum fruitbody the most according to claim 1 and Reishi sporule comprehensive extraction and application production method, is characterized by, the supersonic frequency of described ultrasonic oscillation device is 28 ~ 59KHz, and ultrasonic power is 120 ~ 600W.
A kind of ganoderma lucidum fruitbody the most according to claim 1 and Reishi sporule comprehensive extraction and application production method, it is characterized by, described complex enzyme neutral and alkali protease, chymotrypsin are 10:4:7 with the mass ratio of papain, and the interpolation quality of described complex enzyme is the 0.5 ~ 1% of precipitation quality.
A kind of ganoderma lucidum fruitbody the most according to claim 1 and Reishi sporule comprehensive extraction and application production method, is characterized by that described ganoderma lucidum spore oil and GL-B may use and prepare Ganoderma lucidum, and its preparation method is as follows:
Softgel shell glue the most processed: take gelatin, G & W, after the 15 ~ 20min that at room temperature deaerates stirs, controlling temperature is 60 ~ 70 DEG C of insulation 40 ~ 50min, scrapes off liquid level foam, obtaining softgel shell glue, described gelatin, the weight ratio of G & W are 1:(0.4 ~ 0.6): 1；
Content the most processed: take 10 ~ 20 parts of ganoderma lucidum spore oils, 4 ~ 8 parts of GL-Bs, 3 ~ 4 parts of Radixs Astragali, 2 ~ 3 parts of rhodiola roots, 2 portions of Radix Glycyrrhizaes, 1 ~ 2 part of beeswax, 0.5 part of cassia seed, 0.1 ~ 0.2 portion of gynostemma pentaphylla, 0.1 ~ 0.2 portion of Chinese bulbul, 0.1 part of lutein and 0.01 ~ 0.05 part of vitaminAD according to the mass fraction, it is sequentially added in stirred tank, stir at 40 ~ 55 DEG C, cross colloid mill 2 ~ 3 times, deaerate and i.e. obtain content；
3. prepare Ganoderma lucidum: content and softgel shell glue put into and encapsulating machine is pressed into soft capsule, and the 3 ~ 4h that shapes at 20 ~ 25 DEG C, then wash ball, be dried, check, pack after i.e. obtain Ganoderma lucidum.
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