CN105638221A - Planting method of organic lettuce - Google Patents
Planting method of organic lettuce Download PDFInfo
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- CN105638221A CN105638221A CN201610089155.6A CN201610089155A CN105638221A CN 105638221 A CN105638221 A CN 105638221A CN 201610089155 A CN201610089155 A CN 201610089155A CN 105638221 A CN105638221 A CN 105638221A
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- 238000000034 method Methods 0.000 title claims abstract description 41
- 241000208822 Lactuca Species 0.000 title abstract 6
- 235000003228 Lactuca sativa Nutrition 0.000 title abstract 6
- 210000003608 fece Anatomy 0.000 claims abstract description 43
- 239000010871 livestock manure Substances 0.000 claims abstract description 43
- 230000001580 bacterial effect Effects 0.000 claims abstract description 37
- 239000003814 drug Substances 0.000 claims abstract description 33
- 244000005700 microbiome Species 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 238000009331 sowing Methods 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 60
- 239000002068 microbial inoculum Substances 0.000 claims description 41
- 239000002893 slag Substances 0.000 claims description 37
- 244000063299 Bacillus subtilis Species 0.000 claims description 34
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 34
- 238000000855 fermentation Methods 0.000 claims description 30
- 230000004151 fermentation Effects 0.000 claims description 30
- 238000002513 implantation Methods 0.000 claims description 25
- 239000001963 growth medium Substances 0.000 claims description 23
- 239000002609 medium Substances 0.000 claims description 16
- 241000283690 Bos taurus Species 0.000 claims description 14
- 241000287828 Gallus gallus Species 0.000 claims description 14
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims description 14
- 239000005855 Trichoderma harzianum strains T-22 and ITEM 908 Substances 0.000 claims description 14
- 239000012531 culture fluid Substances 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 11
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 241000223260 Trichoderma harzianum Species 0.000 claims description 8
- 244000060011 Cocos nucifera Species 0.000 claims description 7
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 7
- 244000061456 Solanum tuberosum Species 0.000 claims description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 238000007596 consolidation process Methods 0.000 claims description 7
- 238000005138 cryopreservation Methods 0.000 claims description 7
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 235000013312 flour Nutrition 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 7
- 230000000813 microbial effect Effects 0.000 claims description 7
- DUWWHGPELOTTOE-UHFFFAOYSA-N n-(5-chloro-2,4-dimethoxyphenyl)-3-oxobutanamide Chemical compound COC1=CC(OC)=C(NC(=O)CC(C)=O)C=C1Cl DUWWHGPELOTTOE-UHFFFAOYSA-N 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 235000019260 propionic acid Nutrition 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 210000000582 semen Anatomy 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 201000010099 disease Diseases 0.000 abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 10
- 241000607479 Yersinia pestis Species 0.000 abstract description 9
- 239000002689 soil Substances 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract 1
- 235000013311 vegetables Nutrition 0.000 description 20
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000000796 flavoring agent Substances 0.000 description 5
- 235000019634 flavors Nutrition 0.000 description 5
- 239000007921 spray Substances 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 235000013348 organic food Nutrition 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 240000005860 Portulaca grandiflora Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a planting method of an organic lettuce. The method comprises the following steps that 1, land is leveled and compacted, 3,000-5,000 kg of matured farmyard manure is applied per mu, deep ploughing and raking are conducted, and a flat ridge which is 2-3-meter wide is made; 2, when sowing is conducted, shallow furrows are formed according to the line spacing of 20-25 cm in the ridge surface during sowing, and seeds are evenly scattered in the furrows and covered with fine earth and traditional Chinese medicine residues, wherein the traditional Chinese medicine residues account for 5%-20% by weight percentage of the fine earth; 3, after sowing is conducted, the ridge surface is kept wet, after seedling emergence is conducted, thinning and final singling are conducted in time, and microorganism bacterial liquid is sprayed regularly after seedling emergence, wherein the line spacing is 20-25 cm, and the row spacing is 15 cm. According to the planting method of the organic lettuce, by means of the mixture of the fine earth and the traditional Chinese medicine residues, the nutritional ingredients of the soil can be increased, plant diseases and insect pests can also be reduced, and the yield of the organic lettuce can be increased; by means of the microorganism bacterial liquid, the plant diseases and insect pests can be effectively prevented in the lettuce growth process, and the quality of the organic lettuce is further improved.
Description
Technical field
The present invention relates to the implantation methods of a kind of organic Herba Lactucae versicoloris, belong to organic vegetable field of planting.
Background technology
Organic vegetable refers in vegetable production process in strict accordance with organic production code, do not use the fertilizer of any chemosynthesis, and do not use genetically engineered biological and product thereof, but follow the course of nature and ecological principle, take the agricultural technology of a series of sustainable development, coordinate plantation balance, maintain Agro-ecological System continual and steady, and identify certification through Organic Food Attestation mechanism, and issue the victual of Organic food certificate.
Herba Sonchi Oleracei is raw sun plant in one, likes water, addicted to fertile, not drought-resistant. Like moist, fertile and loose soil, soil from sandy soil to clay, between pH4.5-8.9 all can grow, but best so that micro-acid to neutral sand drift soil to grow, and its cold and dressed with sauce mode of generally individually employing eats, along with growth in the living standard, organic vegetable is increasingly subject to the favor of people. But how it effectively prevents vegetable insect disease, and nutrition effectively provides the technical barrier being to plant organic Herba Lactucae versicoloris.
Summary of the invention
The present invention is to solve that above-mentioned technical problem provides the implantation methods of a kind of organic Herba Lactucae versicoloris.
The technical scheme is that the implantation methods of a kind of organic Herba Lactucae versicoloris, comprise the following steps:
Step 1), by ground leveling, consolidation, execute well-rotted farmyard manure 3,000 5000 kilograms, deep plough and rake for every mu, make the flat furrow that 2-3 rice is wide,
Step 2), during sowing, on furrow face, line-spacing by 20-25 centimetre opens shallow ridges, is then uniformly sprinkled in ditch by seed, and covers the mixture of fine earth and Chinese medicine slag, and described Chinese medicine slag accounts for the 5%-20% of fine earth weight percent;
Step 3), select ripe Herba Lactucae versicoloris seed standby;
Step 4), after planting to ensure that furrow face moistens, timely thinning, final singling, line-spacing 20-25 centimetre, spacing in the rows 15 centimetres, periodically sprinkle microbial inoculum after emerging after emerging.
On the basis of technique scheme, the present invention can also do following improvement.
The implantation methods of the present invention a kind of organic Herba Lactucae versicoloris described above, further, described microbial inoculum is mycete microbial inoculum and/or bacterial micro-organism bacterium solution, specifically: sprayed mycete microbial inoculum every 4 days, bacterial micro-organism bacterium solution, mycete microbial inoculum and bacterial micro-organism bacterium solution fountain height respectively every mu 10-30 kilogram is sprayed every 3 days.
The implantation methods of the present invention a kind of organic Herba Lactucae versicoloris described above, further, described mycete microbial inoculum is prepared by following steps:
Step 4.1), trichoderma harzianum T-22 is inoculated in potato glucose (PD) fluid medium, 25 DEG C-30 DEG C, shaking table revolution 125r/min-150r/min when, cultivate 3-4 days, and to regulate spore concentration be 1 �� 105-6Individual/mL, prepares Trichoderma harzianum bacteria culture fluid;
Step 4.2) carry out solid fermentation: wheat bran, Semen Maydis powder, Caulis et Folium Oryzae, coconut shell flour are added in the triangular flask of 1L according to the ratio of 8:5:6:2, add water so as to after humidity reaches 100% sterilizing standby, by 10mL step 4.1) the trichoderma harzianum T-22 culture fluid that obtains joins in triangular flask and prepares mycete microbial bacterial agent at 25 DEG C-30 DEG C after fermentation culture 3-4 days.
The implantation methods of the present invention a kind of organic Herba Lactucae versicoloris described above, further, described bacterial micro-organism bacterium solution is the bacterium solution of bacillus subtilis S9, and its preparation comprises the following steps:
Step 4.1) the bacillus subtilis S9 strain of cryopreservation is rule on LB culture medium solid plate, then cultivate 20��24 hours at 28 DEG C��32 DEG C, obtain activated spawn;
Step 4.2) according to the ratio that an inoculating loop activated spawn is inoculated in 100mL seed liquor culture medium, activated spawn is inoculated in seed liquor culture medium, 30 DEG C��32 DEG C, 170��210r/min when shaken cultivation 10��15h, obtain seed liquor;
Step 4.3) according to the ratio that percent by volume is 6%��20%, the seed liquor of step (2) gained is inoculated in fermentation medium, 30 DEG C��32 DEG C, pH7.0��7.5,170��210r/min when shaken cultivation 40��50h, obtain fermentation liquid.
Seed culture medium of the present invention can be: Nutrient agar 18g, distilled water 1L, pH6.8��7.0.
Fermentation medium of the present invention can be: selects glucose as carbon source, using peptone, bean as nitrogenous source; Specifically, concentration of glucose is 5g/L, bean 30g/L, and peptone concentration is 2g/L.
The implantation methods of the present invention a kind of organic Herba Lactucae versicoloris described above, further, described Chinese medicine slag includes following weight portion Chinese medicine slag raw material: Rhizoma Atractylodis 10-20 part, Poria 15-18 part, Rhizoma Pinelliae 8-10 part, Pericarpium Citri Reticulatae 30-50 part, Radix Sophorae Flavescentis 16-24 part, Radix Tripterygii Wilfordii 13-15 part, Radix Stemonae 11-15 part Radix Angelicae Dahuricae 15-20 part and Radix Salviae Miltiorrhizae 20-30 part, described Rhizoma Atractylodis, Poria, Rhizoma Pinelliae, Pericarpium Citri Reticulatae, Radix Sophorae Flavescentis, Radix Tripterygii Wilfordii, the Radix Stemonae, the Radix Angelicae Dahuricae and Radix Salviae Miltiorrhizae slag drying and crushing cross 200-300 mesh sieve.
The implantation methods of the present invention a kind of organic Herba Lactucae versicoloris described above, further, described after planting to ensure furrow face moisten be by add 5-20% propanoic acid carry out watering or spraying.
The implantation methods of the present invention a kind of organic Herba Lactucae versicoloris described above, further, described bacterial micro-organism bacterium solution also includes muscardine and bacillus thuringiensis bacterium solution, described muscardine, bacillus thuringiensis bacterium solution preparation method identical with the preparation method of described bacillus subtilis S9, it is 25:13:28 that described bacillus subtilis S9, muscardine and bacillus thuringiensis make the quantitative proportion in mixed bacteria liquid.
The implantation methods of the present invention a kind of organic Herba Lactucae versicoloris described above, further, step 1) described well-rotted farmyard manure be in chicken manure and cattle manure addition bacillus subtilis S9 bacterium solution, the addition quality of described bacillus subtilis S9 bacterium solution is chicken manure and the 12-18% of cattle manure quality.
The invention has the beneficial effects as follows: at the mixture that due to the fact that fine earth and Chinese medicine slag, be possible not only to increase the nutritional labeling of soil and the generation of pest and disease damage can also be reduced, it is possible to increase the yield of organic Herba Sonchi Oleracei; The present invention adopts microorganism microbial inoculum can effectively prevent the generation of pest and disease damage in Herba Lactucae versicoloris growth course, improves the quality of organic Herba Sonchi Oleracei further.
Detailed description of the invention
With the following Examples principles of the invention and feature being described, example is served only for explaining the present invention, is not intended to limit the scope of the present invention.
Trichoderma harzianum T-22 of the present invention, bacillus subtilis S9, muscardine and bacillus thuringiensis are all purchased from Beijing Coway BioWorks BioTech Co., Ltd..
Seed culture medium of the present invention is: Nutrient agar 18g, distilled water 1L, pH6.8��7.0.
Fermentation medium of the present invention is: concentration of glucose is 5g/L, bean 30g/L, and peptone concentration is 2g/L.
Embodiment 1
The implantation methods of a kind of organic Herba Lactucae versicoloris of the present invention, comprises the following steps:
Step 1), by ground leveling, consolidation, execute well-rotted farmyard manure 3000 kilograms for every mu, deep plough and rake, make the flat furrow of 3 meters wide, well-rotted farmyard manure described in the present embodiment is addition bacillus subtilis S9 bacterium solution, addition quality is chicken manure and cattle manure quality the 12% of described bacillus subtilis S9 bacterium solution in chicken manure and cattle manure.
Step 2), during sowing, on furrow face, line-spacing by 20-25 centimetre opens shallow ridges, is then uniformly sprinkled in ditch by seed, and covers the mixture of fine earth and Chinese medicine slag, and described Chinese medicine slag accounts for the 5% of fine earth weight percent; Chinese medicine slag described in the present embodiment includes following Chinese medicine slag raw material: Rhizoma Atractylodis 10kg, Poria 15kg, Rhizoma Pinelliae 8kg, Pericarpium Citri Reticulatae 30kg, Radix Sophorae Flavescentis 16kg, Radix Tripterygii Wilfordii 13kg, Radix Stemonae 11kg, Radix Angelicae Dahuricae 15kg and Radix Salviae Miltiorrhizae 20kg, and described Rhizoma Atractylodis, Poria, Rhizoma Pinelliae, Pericarpium Citri Reticulatae, Radix Sophorae Flavescentis, Radix Tripterygii Wilfordii, the Radix Stemonae, the Radix Angelicae Dahuricae and Radix Salviae Miltiorrhizae slag drying and crushing cross 200 mesh sieves.
Step 3), select ripe Herba Lactucae versicoloris seed standby;
Step 4), after planting to ensure that furrow face moistens, it is ensured that it is undertaken watering or spraying by the propanoic acid of addition 5% that furrow face moistens; Timely thinning, final singling, line-spacing 25 centimetres, spacing in the rows 15 centimetres, periodically sprinkle microbial inoculum after emerging after emerging; Microbial inoculum is mycete microbial inoculum and bacterial micro-organism bacterium solution, mycete microbial inoculum is sprayed every 4 days, spraying bacterial micro-organism bacterium solution every 3 days, and mycete microbial inoculum and bacterial micro-organism bacterium solution interval are sprayed, fountain height is 10 kilograms every mu respectively;
The present embodiment mycete microbial inoculum is prepared by following steps:
Step 4.1), trichoderma harzianum T-22 is inoculated in potato glucose (PD) fluid medium, 25 DEG C-30 DEG C, shaking table revolution 125r/min-150r/min when, cultivate 3-4 days, and to regulate spore concentration be 1 �� 105-6Individual/mL, prepares Trichoderma harzianum bacteria culture fluid;
Step 4.2) carry out solid fermentation: wheat bran, Semen Maydis powder, Caulis et Folium Oryzae, coconut shell flour are added in the triangular flask of 1L according to the ratio of 8:5:6:2, add appropriate water so as to after humidity reaches 100% sterilizing standby, by 10mL step 4.1) the trichoderma harzianum T-22 culture fluid that obtains joins in triangular flask and prepares mycete microbial bacterial agent 25 DEG C of fermentation culture after 4 days.
The above-mentioned bacterial micro-organism bacterium solution of the present embodiment is the bacterium solution of bacillus subtilis S9, and its preparation comprises the following steps:
Step 4.1) the bacillus subtilis S9 strain of cryopreservation is rule on LB culture medium solid plate, then cultivate 20 hours at 28 DEG C, obtain activated spawn;
Step 4.2) according to the ratio that an inoculating loop activated spawn is inoculated in 100mL seed liquor culture medium, activated spawn is inoculated in seed liquor culture medium, 30 DEG C DEG C, 170r/min when shaken cultivation 10h, obtain seed liquor;
Step 4.3) according to the ratio that percent by volume is 6%%, the seed liquor of step (2) gained is inoculated in fermentation medium, 30 DEG C, pH7.0,170r/min when shaken cultivation 40h, obtain fermentation liquid.
The method produces and produces organic vegetable easily, and pest and disease damage, per mu yield 2500kg does not occur in the whole production phase, it is also possible to be used in plastic greenhouse kind, and the yield of vegetables that the method produces is high, quality is good, local flavor is special, meets organic vegetable export standard.
Embodiment 2
The implantation methods of a kind of organic Herba Lactucae versicoloris of the present invention, comprises the following steps:
Step 1), by ground leveling, consolidation, execute well-rotted farmyard manure 5000 kilograms for every mu, deep plough and rake, make the flat furrow of 3 meters wide, well-rotted farmyard manure described in the present embodiment is addition bacillus subtilis S9 bacterium solution, addition quality is chicken manure and cattle manure quality the 18% of described bacillus subtilis S9 bacterium solution in chicken manure and cattle manure.
Step 2), on furrow face, to open shallow ridges by the line-spacing of 25 centimetres during sowing, then seed is uniformly sprinkled in ditch, and cover the mixture of fine earth and Chinese medicine slag, described Chinese medicine slag accounts for the 5%-20% of fine earth weight percent; Chinese medicine slag described in the present embodiment includes following Chinese medicine slag raw material: Rhizoma Atractylodis 20kg, Poria 18kg, Rhizoma Pinelliae 10kg, Pericarpium Citri Reticulatae 50kg, Radix Sophorae Flavescentis 24kg, Radix Tripterygii Wilfordii 15kg, Radix Stemonae 15kg, Radix Angelicae Dahuricae 20kg and Radix Salviae Miltiorrhizae 30kg, and described Rhizoma Atractylodis, Poria, Rhizoma Pinelliae, Pericarpium Citri Reticulatae, Radix Sophorae Flavescentis, Radix Tripterygii Wilfordii, the Radix Stemonae, the Radix Angelicae Dahuricae and Radix Salviae Miltiorrhizae slag drying and crushing cross 300 mesh sieves.
Step 3), select ripe Herba Lactucae versicoloris seed standby;
Step 4), after planting to ensure that furrow face moistens, it is ensured that it is undertaken watering or spraying by the propanoic acid of addition 20% that furrow face moistens; Timely thinning, final singling, line-spacing 25 centimetres, spacing in the rows 15 centimetres, periodically sprinkle microbial inoculum after emerging after emerging; Microbial inoculum is mycete microbial inoculum and/or bacterial micro-organism bacterium solution, mycete microbial inoculum is sprayed every 4 days, spraying bacterial micro-organism bacterium solution every 3 days, and mycete microbial inoculum and bacterial micro-organism bacterium solution interval are sprayed, fountain height is 30 kilograms every mu respectively;
The present embodiment mycete microbial inoculum is prepared by following steps:
Step 4.1), trichoderma harzianum T-22 is inoculated in potato glucose (PD) fluid medium, 30 DEG C, shaking table revolution 125r/min-150r/min when, cultivate 4 days, and to regulate spore concentration be 1 �� 106Individual/mL, prepares Trichoderma harzianum bacteria culture fluid;
Step 4.2) carry out solid fermentation: wheat bran, Semen Maydis powder, Caulis et Folium Oryzae, coconut shell flour are added in the triangular flask of 1L according to the ratio of 8:5:6:2, add appropriate water so as to after humidity reaches 100% sterilizing standby, by 10mL step 4.1) the trichoderma harzianum T-22 culture fluid that obtains joins in triangular flask and prepares mycete microbial bacterial agent 30 DEG C of fermentation culture after 3 days.
Bacterial micro-organism bacterium solution described in the present embodiment is the bacterium solution of bacillus subtilis S9, and its preparation comprises the following steps:
Step 4.1) the bacillus subtilis S9 strain of cryopreservation is rule on LB culture medium solid plate, then cultivate 24 hours at 32 DEG C, obtain activated spawn;
Step 4.2) according to the ratio that an inoculating loop activated spawn is inoculated in 100mL seed liquor culture medium, activated spawn is inoculated in seed liquor culture medium, 32 DEG C, 210r/min when shaken cultivation 15h, obtain seed liquor;
Step 4.3) according to the ratio that percent by volume is 20%, the seed liquor of step (2) gained is inoculated in fermentation medium, 32 DEG C, pH7.5,210r/min when shaken cultivation 50h, obtain fermentation liquid.
The method produces and produces organic vegetable easily, and pest and disease damage, per mu yield 3000kg does not occur in the whole production phase, it is also possible to be used in plastic greenhouse kind, and the yield of vegetables that the method produces is high, quality is good, local flavor is special, meets organic vegetable export standard.
Embodiment 3
The implantation methods of a kind of organic Herba Lactucae versicoloris of the present invention, comprises the following steps:
Step 1), by ground leveling, consolidation, execute well-rotted farmyard manure 5000 kilograms for every mu, deep plough and rake, make the flat furrow of 3 meters wide, well-rotted farmyard manure described in the present embodiment is addition bacillus subtilis S9 bacterium solution, addition quality is chicken manure and cattle manure quality the 18% of described bacillus subtilis S9 bacterium solution in chicken manure and cattle manure.
Step 2), during sowing, on furrow face, line-spacing by 20-25 centimetre opens shallow ridges, is then uniformly sprinkled in ditch by seed, and covers the mixture of fine earth and Chinese medicine slag, and described Chinese medicine slag accounts for the 5%-20% of fine earth weight percent; Chinese medicine slag described in the present embodiment includes following Chinese medicine slag raw material: Rhizoma Atractylodis 18kg, Poria 18kg, Rhizoma Pinelliae 9kg, Pericarpium Citri Reticulatae 35kg, Radix Sophorae Flavescentis 20kg, Radix Tripterygii Wilfordii 14kg, Radix Stemonae 14kg, Radix Angelicae Dahuricae 18kg and Radix Salviae Miltiorrhizae 25kg, and described Rhizoma Atractylodis, Poria, Rhizoma Pinelliae, Pericarpium Citri Reticulatae, Radix Sophorae Flavescentis, Radix Tripterygii Wilfordii, the Radix Stemonae, the Radix Angelicae Dahuricae and Radix Salviae Miltiorrhizae slag drying and crushing cross 200 mesh sieves.
Step 3), select ripe Herba Lactucae versicoloris seed standby;
Step 4), after planting to ensure that furrow face moistens, it is ensured that it is undertaken watering or spraying by the propanoic acid of addition 18% that furrow face moistens; Timely thinning, final singling, line-spacing 25 centimetres, spacing in the rows 15 centimetres, periodically sprinkle microbial inoculum after emerging after emerging; Microbial inoculum is mycete microbial inoculum and/or bacterial micro-organism bacterium solution, sprays mycete microbial inoculum every 4 days, sprays bacterial micro-organism bacterium solution every 3 days, and fountain height is 30 kilograms every mu;
The present embodiment mycete microbial inoculum is prepared by following steps:
Step 4.1), trichoderma harzianum T-22 is inoculated in potato glucose (PD) fluid medium, 30 DEG C, shaking table revolution 150r/min when, cultivate 4 days, and to regulate spore concentration be 1 �� 106Individual/mL, prepares Trichoderma harzianum bacteria culture fluid;
Step 4.2) carry out solid fermentation: wheat bran, Semen Maydis powder, Caulis et Folium Oryzae, coconut shell flour are added in the triangular flask of 1L according to the ratio of 8:5:6:2, add appropriate water so as to after humidity reaches 100% sterilizing standby, by 10mL step 4.1) the trichoderma harzianum T-22 culture fluid that obtains joins in triangular flask and prepares mycete microbial bacterial agent 25 DEG C of fermentation culture after 4 days.
Bacterial micro-organism bacterium solution described in the present embodiment is the bacterium solution of bacillus subtilis S9, and its preparation comprises the following steps:
Step 4.1) the bacillus subtilis S9 strain of cryopreservation is rule on LB culture medium solid plate, then cultivate 20 hours at 28 DEG C, obtain activated spawn;
Step 4.2) according to the ratio that an inoculating loop activated spawn is inoculated in 100mL seed liquor culture medium, activated spawn is inoculated in seed liquor culture medium, 30 DEG C, 170r/min when shaken cultivation 15h, obtain seed liquor;
Step 4.3) according to the ratio that percent by volume is 15%, the seed liquor of step (2) gained is inoculated in fermentation medium, 32 DEG C, pH7.5,210r/min when shaken cultivation 40h, obtain fermentation liquid.
The method produces and produces organic vegetable easily, and pest and disease damage, per mu yield 2500kg does not occur in the whole production phase, it is also possible to be used in plastic greenhouse kind, and the yield of vegetables that the method produces is high, quality is good, local flavor is special, meets organic vegetable export standard.
Embodiment 4
The implantation methods of a kind of organic Herba Lactucae versicoloris of the present invention, comprises the following steps:
Step 1), by ground leveling, consolidation, execute well-rotted farmyard manure 4500 kilograms for every mu, deep plough and rake, make the flat furrow of 3 meters wide, well-rotted farmyard manure described in the present embodiment is addition bacillus subtilis S9 bacterium solution, addition quality is chicken manure and cattle manure quality the 12% of described bacillus subtilis S9 bacterium solution in chicken manure and cattle manure.
Step 2), on furrow face, to open shallow ridges by the line-spacing of 25 centimetres during sowing, then seed is uniformly sprinkled in ditch, and cover the mixture of fine earth and Chinese medicine slag, described Chinese medicine slag accounts for the 5%-20% of fine earth weight percent; Chinese medicine slag described in the present embodiment includes following Chinese medicine slag raw material: Rhizoma Atractylodis 20kg, Poria 15kg, Rhizoma Pinelliae 8kg, Pericarpium Citri Reticulatae 50kg, Radix Sophorae Flavescentis 24kg, Radix Tripterygii Wilfordii 13kg, Radix Stemonae 15kg, Radix Angelicae Dahuricae 15kg and Radix Salviae Miltiorrhizae 20kg, and described Rhizoma Atractylodis, Poria, Rhizoma Pinelliae, Pericarpium Citri Reticulatae, Radix Sophorae Flavescentis, Radix Tripterygii Wilfordii, the Radix Stemonae, the Radix Angelicae Dahuricae and Radix Salviae Miltiorrhizae slag drying and crushing cross 250 mesh sieves.
Step 3), select ripe Herba Lactucae versicoloris seed standby;
Step 4), after planting to ensure that furrow face moistens, it is ensured that it is undertaken watering or spraying by the propanoic acid of addition 10% that furrow face moistens; Timely thinning, final singling, line-spacing 25 centimetres, spacing in the rows 15 centimetres, periodically sprinkle microbial inoculum after emerging after emerging; Microbial inoculum is mycete microbial inoculum and/or bacterial micro-organism bacterium solution, sprays mycete microbial inoculum every 4 days, sprays bacterial micro-organism bacterium solution every 3 days, and fountain height is 10 kilograms every mu;
The present embodiment mycete microbial inoculum is prepared by following steps:
Step 4.1), trichoderma harzianum T-22 is inoculated in potato glucose (PD) fluid medium, 25 DEG C, shaking table revolution 125r/min when, cultivate 3 days, and to regulate spore concentration be 1 �� 106Individual/mL, prepares Trichoderma harzianum bacteria culture fluid;
Step 4.2) carry out solid fermentation: wheat bran, Semen Maydis powder, Caulis et Folium Oryzae, coconut shell flour are added in the triangular flask of 1L according to the ratio of 8:5:6:2, add appropriate water so as to after humidity reaches 100% sterilizing standby, by 10mL step 4.1) the trichoderma harzianum T-22 culture fluid that obtains joins in triangular flask and prepares mycete microbial bacterial agent 30 DEG C of fermentation culture after 4 days.
Bacterial micro-organism bacterium solution described in the present embodiment is the bacterium solution of bacillus subtilis S9, and its preparation comprises the following steps:
Step 4.1) the bacillus subtilis S9 strain of cryopreservation is rule on LB culture medium solid plate, then cultivate 20 hours at 28 DEG C, obtain activated spawn;
Step 4.2) according to the ratio that an inoculating loop activated spawn is inoculated in 100mL seed liquor culture medium, activated spawn is inoculated in seed liquor culture medium, 32 DEG C, 210r/min when shaken cultivation 10, obtain seed liquor;
Step 4.3) according to the ratio that percent by volume is 8%, the seed liquor of step (2) gained is inoculated in fermentation medium, 32 DEG C, pH7.0��7.5,210r/min when shaken cultivation 50h, obtain fermentation liquid.
The method produces and produces organic vegetable easily, and pest and disease damage, per mu yield 2800kg does not occur in the whole production phase, it is also possible to be used in plastic greenhouse kind, and the yield of vegetables that the method produces is high, quality is good, local flavor is special, meets organic vegetable export standard.
Embodiment 5
The implantation methods of a kind of organic Herba Lactucae versicoloris of the present invention, comprises the following steps:
Step 1), by ground leveling, consolidation, execute well-rotted farmyard manure 4000 kilograms for every mu, deep plough and rake, make the flat furrow of 2 meters wide, well-rotted farmyard manure described in the present embodiment is addition bacillus subtilis S9 bacterium solution, addition quality is chicken manure and cattle manure quality the 15% of described bacillus subtilis S9 bacterium solution in chicken manure and cattle manure.
Step 2), on furrow face, to open shallow ridges by the line-spacing of 22 centimetres during sowing, then seed is uniformly sprinkled in ditch, and cover the mixture of fine earth and Chinese medicine slag, described Chinese medicine slag accounts for the 5%-20% of fine earth weight percent;Chinese medicine slag described in the present embodiment includes following Chinese medicine slag raw material: Rhizoma Atractylodis 15kg, Poria 17kg, Rhizoma Pinelliae 10kg, Pericarpium Citri Reticulatae 35kg, Radix Sophorae Flavescentis 20kg, Radix Tripterygii Wilfordii 14kg, Radix Stemonae 14kg, Radix Angelicae Dahuricae 18kg and Radix Salviae Miltiorrhizae 28kg, and described Rhizoma Atractylodis, Poria, Rhizoma Pinelliae, Pericarpium Citri Reticulatae, Radix Sophorae Flavescentis, Radix Tripterygii Wilfordii, the Radix Stemonae, the Radix Angelicae Dahuricae and Radix Salviae Miltiorrhizae slag drying and crushing cross 200 mesh sieves.
Step 3), select ripe Herba Lactucae versicoloris seed standby;
Step 4), after planting to ensure that furrow face moistens, it is ensured that it is undertaken watering or spraying by the propanoic acid of addition 6% that furrow face moistens; Timely thinning, final singling, line-spacing 20 centimetres, spacing in the rows 15 centimetres, periodically sprinkle microbial inoculum after emerging after emerging; Microbial inoculum is mycete microbial inoculum and/or bacterial micro-organism bacterium solution, mycete microbial inoculum is sprayed every 4 days, spraying bacterial micro-organism bacterium solution every 3 days, and mycete microbial inoculum and bacterial micro-organism bacterium solution interval are sprayed, fountain height is 30 kilograms every mu respectively;
The present embodiment mycete microbial inoculum is prepared by following steps:
Step 4.1), trichoderma harzianum T-22 is inoculated in potato glucose (PD) fluid medium, 25 DEG C-30 DEG C, shaking table revolution 125r/minr/min when, cultivate 3-4 days, and to regulate spore concentration be 1 �� 106Individual/mL, prepares Trichoderma harzianum bacteria culture fluid;
Step 4.2) carry out solid fermentation: wheat bran, Semen Maydis powder, Caulis et Folium Oryzae, coconut shell flour are added in the triangular flask of 1L according to the ratio of 8:5:6:2, add appropriate water so as to after humidity reaches 100% sterilizing standby, by 10mL step 4.1) the trichoderma harzianum T-22 culture fluid that obtains joins in triangular flask and prepares mycete microbial bacterial agent 30 DEG C of fermentation culture after 4 days.
Bacterial micro-organism bacterium solution described in the present embodiment is the bacterium solution of bacillus subtilis S9, muscardine and bacillus thuringiensis bacterium solution, and it is 25:13:28 that described bacillus subtilis S9, muscardine and bacillus thuringiensis make the quantitative proportion in mixed bacteria liquid.
Its preparation comprises the following steps:
Step 4.1) the bacillus subtilis S9 of cryopreservation, muscardine and bacillus thuringiensis strain are rule on LB culture medium solid plate, then cultivate 24 hours at 32 DEG C, obtain activated spawn;
Step 4.2) according to the ratio that an inoculating loop activated spawn is inoculated in 100mL seed liquor culture medium, activated spawn is inoculated in seed liquor culture medium, 32 DEG C, 170r/min when shaken cultivation 15h, obtain seed liquor;
Step 4.3) according to the ratio that percent by volume is 12%, the seed liquor of step (2) gained is inoculated in fermentation medium, 30 DEG C, pH7.5,210r/min when shaken cultivation 50h, obtain fermentation liquid.
The method produces and produces organic vegetable easily, and pest and disease damage, per mu yield 3000kg does not occur in the whole production phase, it is also possible to be used in plastic greenhouse kind, and the yield of vegetables that the method produces is high, quality is good, local flavor is special, meets organic vegetable export standard.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (9)
1. the implantation methods of an organic Herba Lactucae versicoloris, it is characterised in that comprise the following steps:
Step 1), by ground leveling, consolidation, execute well-rotted farmyard manure 3000-5000 kilogram for every mu, deep plough and rake, make the flat furrow that 2-3 rice is wide,
Step 2), during sowing, on furrow face, line-spacing by 20-25 centimetre opens shallow ridges, is then uniformly sprinkled in ditch by seed, and covers the mixture of fine earth and Chinese medicine slag, and described Chinese medicine slag accounts for the 5%-20% of fine earth weight percent;
Step 3), select ripe Herba Lactucae versicoloris seed standby;
Step 4), after planting to ensure that furrow face moistens, timely thinning, final singling, line-spacing 20-25 centimetre, spacing in the rows 15 centimetres, periodically sprinkle microbial inoculum after emerging after emerging.
2. the implantation methods according to a claim 1 organic Herba Lactucae versicoloris, it is characterized in that, described microbial inoculum is mycete microbial inoculum and/or bacterial micro-organism bacterium solution, specifically: sprayed mycete microbial inoculum every 4 days, bacterial micro-organism bacterium solution, mycete microbial inoculum and bacterial micro-organism bacterium solution fountain height respectively every mu 10-30 kilogram is sprayed every 3 days.
3. the implantation methods according to a claim 2 organic Herba Lactucae versicoloris, it is characterised in that described mycete microbial inoculum is prepared by following steps:
Step 4.1), trichoderma harzianum T-22 is inoculated in potato glucose (PD) fluid medium, 25 DEG C-30 DEG C, shaking table revolution 125r/min-150r/min when, cultivate 3-4 days, and to regulate spore concentration be 1 �� 105-6Individual/mL, prepares Trichoderma harzianum bacteria culture fluid;
Step 4.2), carry out solid fermentation: wheat bran, Semen Maydis powder, Caulis et Folium Oryzae, coconut shell flour are added in the triangular flask of 1L according to the ratio of 8:5:6:2, add water so as to after humidity reaches 100% sterilizing standby, by 10mL step 4.1) the Trichoderma harzianum bacteria culture fluid that obtains joins in triangular flask and prepares mycete microbial bacterial agent at 25 DEG C-30 DEG C after fermentation culture 3-4 days.
4. the implantation methods according to a claim 2 organic Herba Lactucae versicoloris, it is characterised in that described bacterial micro-organism bacterium solution is the bacterium solution of bacillus subtilis S9, its preparation comprises the following steps:
Step 4.1) the bacillus subtilis S9 strain of cryopreservation is rule on LB culture medium solid plate, then cultivate 20��24 hours at 28 DEG C��32 DEG C, obtain activated spawn;
Step 4.2) according to by an inoculating loop step 4.1) the described activated spawn prepared is inoculated in the ratio of 100mL seed liquor culture medium and activated spawn is inoculated in seed liquor culture medium, 30 DEG C��32 DEG C, 170��210r/min when shaken cultivation 10��15h, obtain seed liquor;
Step 4.3) according to the ratio that percent by volume is 6%��20%, the seed liquor of step (2) gained is inoculated in fermentation medium, 30 DEG C��32 DEG C, pH7.0��7.5,170��210r/min when shaken cultivation 40��50h, obtain fermentation liquid.
5. the implantation methods of a kind of organic Herba Lactucae versicoloris according to any one of Claims 1-4, it is characterized in that, step 2) described in Chinese medicine slag include following weight portion Chinese medicine slag raw material: Rhizoma Atractylodis 10-20 part, Poria 15-18 part, Rhizoma Pinelliae 8-10 part, Pericarpium Citri Reticulatae 30-50 part, Radix Sophorae Flavescentis 16-24 part, Radix Tripterygii Wilfordii 13-15 part, Radix Stemonae 11-15 part, Radix Angelicae Dahuricae 15-20 part and Radix Salviae Miltiorrhizae 20-30 part, described Rhizoma Atractylodis, Poria, Rhizoma Pinelliae, Pericarpium Citri Reticulatae, Radix Sophorae Flavescentis, Radix Tripterygii Wilfordii, the Radix Stemonae, the Radix Angelicae Dahuricae and Radix Salviae Miltiorrhizae slag drying and crushing cross 200-300 mesh sieve.
6. the implantation methods of a kind of organic Herba Lactucae versicoloris according to any one of Claims 1-4, it is characterised in that described after planting to ensure furrow face moisten be by add 5-20% propanoic acid carry out watering or spraying.
7. the implantation methods according to a claim 4 organic Herba Lactucae versicoloris, it is characterized in that, described bacterial micro-organism bacterium solution also includes muscardine and bacillus thuringiensis bacterium solution, described muscardine, bacillus thuringiensis bacterium solution preparation method identical with the preparation method of described bacillus subtilis S9, it is 25:13:28 that described bacillus subtilis S9, muscardine and bacillus thuringiensis make the quantitative proportion in mixed bacteria liquid.
8. the implantation methods according to a claim 4 organic Herba Lactucae versicoloris, it is characterized in that, described well-rotted farmyard manure is addition bacillus subtilis S9 bacterium solution in chicken manure and cattle manure, and the addition quality of described bacillus subtilis S9 bacterium solution is chicken manure and the 12-18% of cattle manure quality.
9. the implantation methods of a kind of organic Herba Lactucae versicoloris according to any one of Claims 1-4, it is characterised in that the thickness of earth covering of described fine earth and Chinese medicine slag is 0.2-1 centimetre.
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| CN109197429A (en) * | 2018-09-07 | 2019-01-15 | 广西大学 | A method of using sugar refinery give up material production wormcast cultivation Brassica rapa L |
| CN109197429B (en) * | 2018-09-07 | 2021-06-25 | 广西大学 | Method for cultivating Shanghai green by utilizing wormcast produced by sugar refinery waste materials |
| CN115843639A (en) * | 2022-12-23 | 2023-03-28 | 北京联合大学 | Method for improving fructification rate of rhizoma atractylodis and application thereof |
| CN116530361A (en) * | 2023-07-03 | 2023-08-04 | 内蒙古绿新园林绿化有限责任公司 | Greenhouse planting production method for Morchella in winter and spring |
| CN116530361B (en) * | 2023-07-03 | 2023-09-15 | 内蒙古绿新园林绿化有限责任公司 | Greenhouse planting production method for Morchella in winter and spring |
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