CN105624082B - 表达单链抗体scFV或多肽适配体的功能化细菌磁颗粒及其应用 - Google Patents
表达单链抗体scFV或多肽适配体的功能化细菌磁颗粒及其应用 Download PDFInfo
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- CN105624082B CN105624082B CN201610153804.4A CN201610153804A CN105624082B CN 105624082 B CN105624082 B CN 105624082B CN 201610153804 A CN201610153804 A CN 201610153804A CN 105624082 B CN105624082 B CN 105624082B
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- magnetic particle
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- membrane protein
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
- C12N15/03—Bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610153804.4A CN105624082B (zh) | 2016-03-17 | 2016-03-17 | 表达单链抗体scFV或多肽适配体的功能化细菌磁颗粒及其应用 |
Applications Claiming Priority (1)
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CN201610153804.4A CN105624082B (zh) | 2016-03-17 | 2016-03-17 | 表达单链抗体scFV或多肽适配体的功能化细菌磁颗粒及其应用 |
Publications (2)
Publication Number | Publication Date |
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CN105624082A CN105624082A (zh) | 2016-06-01 |
CN105624082B true CN105624082B (zh) | 2021-08-24 |
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CN201610153804.4A Active CN105624082B (zh) | 2016-03-17 | 2016-03-17 | 表达单链抗体scFV或多肽适配体的功能化细菌磁颗粒及其应用 |
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CN (1) | CN105624082B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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KR102085101B1 (ko) * | 2018-08-30 | 2020-03-05 | 주식회사 넥스모스 | L-아스코르브산에 특이적으로 결합하는 압타머 및 그 용도 |
CN108165545B (zh) * | 2018-01-22 | 2020-04-21 | 北京国科融智生物技术有限公司 | 一种基于硅基多肽的生物纳米磁珠制备及其应用 |
CN108251415B (zh) * | 2018-01-22 | 2020-05-15 | 北京中科圆融生物科技发展有限公司 | 一种阴离子多肽羧基化生物纳米磁珠及其制备方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104278048B (zh) * | 2013-07-11 | 2017-05-17 | 中国农业大学 | 一种重组磁螺菌及其应用 |
CN104774844A (zh) * | 2015-04-16 | 2015-07-15 | 哈尔滨博翱生物医药技术开发有限公司 | 一种利用细菌表面展示系统筛选单链抗体的方法 |
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2016
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Effective date of registration: 20210926 Address after: 102200 Beijing Changping District science and Technology Park, Zhenxing Road, 36, Cage 2, 6, 625, 627, 629, 631, 633, 635, 637, and 637, respectively. Patentee after: BEIJING GUO KE RONG ZHI BIOTECHNOLOGY Co.,Ltd. Address before: 602, 6 / F, building 2, yard 106, Kexing West Road, Huilongguan town, Changping District, Beijing Patentee before: BEIJING ZHONGKE YUANRONG BIOLOGICAL TECHNOLOGY DEVELOPMENT Co.,Ltd. |
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Effective date of registration: 20230816 Address after: Building 3, Building 1602, Haipurui Biomedical Ecological Park, No.1 Rongtian Road, Jinsha Community, Kengzi Street, Pingshan District, Shenzhen City, Guangdong Province, 518000 Patentee after: Guoke Rongzhi (Shenzhen) Bioengineering Technology Co.,Ltd. Address before: 102200 Beijing Changping District science and Technology Park, Zhenxing Road, 36, Cage 2, 6, 625, 627, 629, 631, 633, 635, 637, and 637, respectively. Patentee before: BEIJING GUO KE RONG ZHI BIOTECHNOLOGY Co.,Ltd. |