CN105596243B - A kind of Essence and facial mask containing nanogold and thermal water - Google Patents

Abstract

The invention discloses a kind of Essence and the facial mask as made from the Essence containing nano gold sol and thermal water (especially Nanjing of China Tangshan Hot Springs water), the Essence and facial mask have the function that whitening weighing apparatus skin and go wrinkle anti-aging.

Description

Essence containing nanogold and hot spring water and facial mask

Technical Field

The invention relates to the field of beauty treatment, and in particular relates to essence containing nanogold and hot spring water (in particular to the hot spring water of the Nanjing Tangshan of China), a facial mask prepared from the essence and a corresponding preparation method.

Background

The facial mask is a category of skin care products, not only can make up the shortage of skin cleaning by makeup removal and face washing, but also can carry out nursing such as water replenishing, moisture preserving, whitening, aging resisting and the like on the skin, and the cosmetic principle is that the skin is temporarily isolated from the outside air by covering the skin for a short time, the skin temperature is simultaneously improved, skin pores are expanded to promote secretion and metabolism of sweat glands, the oxygen content of the skin is increased, and the skin is promoted to remove products of epidermal cell metabolism and accumulated grease substances; the water in the facial mask permeates into horny layer of epidermis to make skin soft, bright and elastic.

Modern beauty masks advocate subtracting skin, namely deeply cleaning, exfoliating and removing oxidized oil; then the skin is added, namely, the skin is subjected to the maintenance work of whitening, aging resistance and the like on the basis of water replenishing and moisture retention.

At present, the facial masks sold in the market are various, most of the facial masks take artificially synthesized chemicals as active ingredients, and although the facial masks have remarkable beautifying effect in a short time, the facial masks can cause skin damage due to the residue of a large amount of chemicals, particularly heavy metals after long-term use.

In the prior art, a beauty product which takes nano-gold, natural plant extracts and artificial additives as main components exists, for example, Chinese patent CN103479551A discloses a toner and a preparation method thereof, and the formula of the components is as follows: phase A: 13 percent of nano gold sol with the nano gold concentration of 400 mu mol/L, 5 percent of butanediol, 0.5 percent of disodium hydrogen phosphate and a proper amount of water; phase B: 0.5% of natural methyl proline, 0.5% of PHOSPHOVITALTM 4%, 0.5% of ferulic acid, 1% of tocopheryl succinate, 0.8% of rosmarinic acid, 0.5% of calendula extract, 0.5% of trehalose and 2% of mucopolysaccharide (10%); and C phase: 0.1% of PCA sodium, 0.05% of rose hydrosol, 0.1% of seaweed extract and 0.1% of phenoxyethanol, but the water used by the toner is common purified water, has no obvious effect on calming the skin of a human body, and is insufficient in supplementing trace elements to the skin.

Therefore, there is a need to develop a facial mask which can provide sufficient trace elements for human skin, has little damage to the skin and has good beautifying effect.

Disclosure of Invention

In order to solve the above problems, the present inventors have conducted intensive studies and, as a result, have found that: the nano gold sol with the particle size of 1-100 nm is doped into the skin care product, so that the absorption of the skin to active ingredients in the skin care product can be improved, and meanwhile, the nano gold sol can promote the proliferation of skin cells of a human body after being covered on the surface of the skin of the human body along with the skin care product, wherein the nano gold sol prepared by a thermal decomposition method avoids strong acid residue in the nano gold sol, so that the damage to the skin of the human body is small; the inventor also finds that the hot spring water, especially the hot spring water of Nanjing Tangshan of China contains radon gas, metasilicic acid, sulfate, strontium element, selenium element and the like, and the content of the substances is suitable for improving the skin condition of a human body, so that the invention selects nano gold sol and hot spring water prepared by a thermal decomposition method, especially the hot spring water of Nanjing Tangshan of China as basic components, and then adds natural plant extracts to avoid adding other chemical reagents which easily cause skin blockage, allergy, poison and even potential carcinogenic threats to prepare essence liquid, and the essence liquid or the mask prepared by the essence liquid has the effects of whitening, balancing skin, supplementing water, preserving moisture, removing wrinkles, resisting aging and the like, thereby completing the invention.

The object of the present invention is to provide the following:

in a first aspect, the invention provides a whitening and skin balancing essence, which is characterized by comprising the following components in parts by weight based on the total weight of the essence:

wherein,

the particle size of the nano-gold in the nano-gold sol is 1-100 nm, and based on the total weight of the nano-gold sol, the weight fraction of the nano-gold is 0.0001-10% by weight of the nano-gold.

In a second aspect, the invention also provides a whitening and skin balancing mask, which is characterized by comprising a mask carrier and whitening and skin balancing essence adsorbed on the mask carrier, wherein,

the mask carrier comprises artificial fiber cloth, natural fiber cloth and blended cloth;

the whitening and skin balancing essence is as described in the first aspect above.

In a third aspect, the invention also provides a wrinkle-removing and anti-aging essence which is characterized by comprising the following components in parts by weight based on the total weight of the essence:

wherein,

the particle size of the nano-gold in the nano-gold sol is 1-100 nm, based on the total weight of the nano-gold sol, wherein the weight fraction of the nano-gold is 0.0001-10%.

In a fourth aspect, the invention also provides a wrinkle-removing and anti-aging mask, which is characterized by comprising a mask carrier and wrinkle-removing and anti-aging essence adsorbed on the mask carrier, wherein,

the mask carrier comprises artificial fiber cloth, natural fiber cloth and blended cloth;

the wrinkle-removing and anti-aging essence is as described in the third aspect above.

Detailed Description

The features and advantages of the present invention will become more apparent and appreciated from the following detailed description of the invention.

The nanogold used in the prior art is usually prepared by reacting chloroauric acid with strong acid, so that the strong acid is inevitably remained in nanogold sol, and the remained strong acid has harm to the skin of a human body, while the nanogold sol used in the invention is prepared by thermally decomposing chloroauric acid by a sol-gel-template method instead of using the strong acid in the preparation process, and the specific preparation method is as described in the preparation of nanogold and the application initial research in cosmetics, and the nano technology 2010(5)12-17, so that the essence prepared in the invention and the mask prepared from the essence have no harm to the skin.

In the nano gold sol used in the invention, the particle size of nano gold is 1-100 nm, preferably 5-50 nm.

The weight fraction of the nano gold in the nano gold sol used in the invention is 0.0001-10%, preferably 0.01-8%, based on the total weight of the nano gold sol.

The inventor finds that after the nano gold sol is added into the essence, the absorption rate of the skin to active ingredients in the essence is improved, and the effects of whitening, balancing, moisturizing, removing wrinkles and resisting aging of the essence on the skin are obviously enhanced; the inventor finds that the nano gold sol can promote the proliferation of skin cells of a human body and promote metabolism after being covered on the surface of the skin of the human body along with the essence, and the nano gold sol has no toxicity to the skin cells (see experiment example 1 specifically).

The hot spring water of the invention, especially China Nanjing Tangshan hot spring is a composite high-temperature mineralized high-quality medical and health care mineral spring of radon, strontium, metasilicic acid, sulfate and the like, the temperature of the hot spring water is up to 60-70 ℃, the hot spring water contains rich elements of strontium, radon, selenium, calcium, magnesium, sulfur, chlorine, iron, sodium and the like, the pH value is about 7, the average value of the mineralization years is about 1.845g/L, and the hot spring water contains the following components in concentration,

wherein,

radon gas contained in hot spring water of Nanjing Tangshan of China can promote the contraction and expansion of skin blood vessels, promote skin metabolism, and take away garbage on the surface of the skin or even at the deep layer of the skin in the process of metabolism, thereby achieving the purpose of beautifying and cleaning the skin.

In the aging process, the growth of connective tissues is obvious, the growth of connective tissues is closely related to the silicon deficiency, and when the skin lacks silicon, the connective tissues obviously grow, thereby causing aging, so that the skin can be prevented from aging by supplementing the silicon in the skin.

In the invention, metasilicic acid in the hot spring water of Nanjing Tangshan is easy to be absorbed by the skin, and silicon in the water is supplemented into the skin to prevent the growth of connective tissues, so that the aging of the skin is delayed.

Selenium element in hot spring water of Nanjing Tangshan of China can clean skin, remove scars, lighten color spots, keep the vitality of organism cells, show the effect of resisting free radicals, have good promotion effect on the growth of epithelial tissues, and have the effects of accelerating the differentiation and proliferation of epidermal cells, promoting the normal arrangement of collagen fibers and accelerating the softening of scar tissues.

The sulfate in the hot spring water of Nanjing Tangshan of China is mainly sodium sulfate, calcium sulfate and ferric sulfate, and the sulfate covers the surface of the skin to relieve the skin and calm nerves.

The inventor finds that the mineral elements in hot spring water of Nanjing Tangshan have a proper proportion, have a promoting effect on moisturizing and whitening, and have an obvious improvement effect on aged, mature, nutrient-deficient or environmentally damaged skin by being matched with nano-gold sol for use.

The vitamins comprise vitamin A, vitamin C and vitamin E, wherein the vitamin A and the vitamin E have the anti-aging effect, and the vitamin E is low in price and easy to produce; on one hand, the vitamin C has good whitening effect on the skin and good reducibility, can remove free radicals on the surface of the skin and has an anti-aging effect; on the other hand, vitamin C can also keep the iron element in the hot spring water to be divalent, and the divalent iron can keep the skin to be ruddy and glossy, and can whiten the skin and promote the blood color by covering the skin with essence or facial mask, so that the vitamin C and the vitamin E are preferably added into the essence.

When the weight ratio of the vitamins in the essence is 0.01-0.50% based on the total weight of the essence, the effect of improving the skin is obvious, and therefore, the invention is preferably 0.02-0.3%, more preferably 0.03-0.2%, such as 0.05%.

The bioactive substance is used for resisting oxidation and protecting protein substances in essence, and is selected from hyaluronic acid and trehalose, preferably trehalose.

The natural whitening substance is used for improving skin pigmentation, removing freckles and whitening, and is selected from tranexamic acid and ferulic acid, preferably ferulic acid.

The moisturizer is used for moisturizing the skin and relieving the dryness of the skin, and is selected from natural proline methyl aminoacetate, butanediol, polyglutamic acid, hyaluronic acid, sodium pyrrolidone carboxylate, urea and lactic acid, preferably natural proline methyl aminoacetate, butanediol and polyglutamic acid;

the skin penetrant is used for promoting absorption of nutrients in the essence, and is selected from decyl methyl sulfoxide (DCMS), azone, 2-pyrrolidone, 5-methyl-2-pyrrolidone, 1, 5-dimethyl-2-pyrrolidone, oleic acid, isopropyl myristate, propylene glycol dinonyl ester and diethyl sebacate, preferably selected from azone, 2-pyrrolidone, 5-methyl-2-pyrrolidone and 1, 5-dimethyl-2-pyrrolidone, more preferably azone;

the buffer is used for maintaining the pH of the essence stable and enabling the essence to be suitable for covering the skin, and is acid salt of phosphoric acid, preferably selected from sodium dihydrogen phosphate, disodium hydrogen phosphate, potassium dihydrogen phosphate and dipotassium hydrogen phosphate, and more preferably sodium dihydrogen phosphate;

the cutin softening agent is used for softening cutin on the surface of skin and enabling the cutin to be easily removed, and is selected from salicylic acid, coal tar and propylene glycol, and is preferably propylene glycol;

the preservative is used for keeping the plant extract/extractive in the essence fresh and slowing down the deterioration of the essence, and is nipagin ester, preferably methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, n-butyl p-hydroxybenzoate and isobutyl p-hydroxybenzoate, and more preferably methyl p-hydroxybenzoate.

According to a first aspect of the invention, the whitening and skin balancing mask containing nanogold and hot spring water is provided, wherein the essence comprises the following components in parts by weight based on the total weight of the essence:

preferably, the first and second liquid crystal materials are,

the raw material medicine of the rhodiola rosea extracting solution is dry roots, rhizomes or whole grass of rhodiola crenulata of Crassulaceae;

the extraction method of the rhodiola rosea extract refers to the method in CN103814954A, and the adopted extraction solvent is 95% ethanol solution, wherein, the rhodiola rosea extract contains 0.5-2 wt% of rhodiola rosea glycoside based on the total weight of the rhodiola rosea extract.

The raw material medicine of the acanthopanax root extracting solution is the root and the rhizome of acanthopanax root of the plant of the acanthopanax of the araliaceae;

the extraction method of the acanthopanax senticosus extract refers to the method described in CN102600221A, and the adopted extraction solvent is 60% ethanol solution, wherein the acanthopanax senticosus extract contains eleutheroside B + E0.05wt% -0.15 wt% based on the total weight of the acanthopanax senticosus extract;

the raw material medicine of the calendula extract is the flower of calendula of Compositae;

the extraction method of the calendula officinalis extract refers to the method described in CN101413020A, the adopted soaking solvent is absolute ethyl alcohol, and the extraction solvent is normal hexane, wherein the calendula officinalis extract comprises the following components in percentage by weight based on the total weight of the calendula officinalis extract:

0.01 to 0.03 weight percent of lutein

0.02 wt% -0.10 wt% of zeaxanthin.

The herba Saxifragae extract is prepared from leaf of Saxifraga stolonifera of Saxifragaceae,

according to the method for extracting the saxifrage extract, referring to the influence of the saxifrage extract on prostate cancer cell apoptosis, the method described in basic medical journal of Chinese medicine 2005, 12(11), ethyl acetate is adopted as an extraction solvent, wherein the total weight of the saxifrage extract is calculated by the sum of the weights of bergenin, quercitrin, quercetin and gallic acid, and the weight fraction of the bergenin, the quercitrin, the quercetin and the gallic acid is 1-5 wt%.

The rhodiola rosea extract, the acanthopanax root extract, the calendula extract and the saxifrage extract provide SOD substances for the whitening and skin balancing essence, so that the whitening, moisturizing and moisturizing effects of the whitening and skin changing essence are enhanced.

The whitening and skin balancing essence is prepared by the following steps:

(1-1) mixing hot spring water with water, adding natural whitening substances, humectant, radix Rhodiolae extract, radix Acanthopanacis Senticosi extract and herba Sidae Rhombifoliae extract, simultaneously adding vitamin C and skin penetrating agent, and adding buffer to obtain mixture I;

(1-2) dissolving saxifrage extract and trehalose in glycerol, adding nanogold sol, stirring, and adding vitamin E/vitamin A, skin penetrating agent and cutin softener to obtain a mixture II;

(1-3) adding the mixture I dropwise to the mixture II with continuous stirring.

The application method of the whitening and skin balancing essence comprises the steps of cleaning the face, and then coating a proper amount of essence on the surface of the skin.

According to a second aspect of the invention, the whitening and skin balancing mask is characterized by comprising a mask carrier and whitening and skin balancing essence adsorbed on the mask carrier, wherein,

the mask carrier comprises artificial fiber cloth, natural fiber cloth and blended cloth;

the whitening and skin balancing essence is the whitening and skin balancing essence of the first aspect.

The whitening and skin balancing mask is prepared by soaking a mask carrier in the whitening and skin balancing essence, and preferably soaking for more than 4 hours.

The application method of the whitening and skin balancing mask comprises the steps of cleaning the face, covering the mask on the surface of the skin, and preferably covering for 15-20 minutes.

According to a third aspect of the invention, the wrinkle-removing and anti-aging essence is characterized by being prepared from the following components in parts by weight:

wherein,

the raw material medicine of the ginseng extract is dried root of ginseng of Panax of Araliaceae;

the extraction method of the ginseng extract is the extraction method referred to CN102961423B, and the extraction solvents are 75%, 85% and 95% ethanol solutions, wherein the total weight of the rhodiola rosea extract is basis, and the ginseng extract contains ginsenoside A + B5-15 mg/L;

the raw material medicine of the medlar extracting solution is mature fruits of Ningxia medlar which is a solanaceae plant;

the extraction method of the medlar extract is the extraction method described in reference to CN1263108A, and comprises the steps of suspending and filtering a 500-mesh terylene filter cloth for a medlar stock solution after seed removal, wherein an elution solvent for column chromatography is double distilled water, and the medlar extract comprises the following components in parts by weight based on the total weight of the medlar extract:

5-10 g/mL of lycium barbarum polysaccharide;

5-10 g/mL of alanine;

the herba Erodii seu Geranii is whole plant with fruit such as herba Geranii of Sophora Japonica family, herba Erodii seu Geranii, and radix Erodii seu Geranii;

the extraction method of the geranium extract refers to the extraction method described in CN1191262C, and the adopted extraction solvent is 50% ethanol solution, and the elution solvent is 75% ethanol solution, wherein the geranium extract contains 10 wt% -15 wt% of flavone based on the total weight of the geranium extract.

The raw material medicine of the seaweed extract is the whole plant of sargassum fusiforme or sargassum pallidum of sargassum family;

the extraction method of the seaweed extract refers to the extraction method described in CN104069059A, and the adopted extraction solvent is 50% ethanol, wherein, based on the total weight of the seaweed extract, the seaweed extract contains 0.02 wt% -0.05 wt% of alginic acid.

The ginseng extract, the medlar extract, the geranium extract and the seaweed extract provide SOD substances for the wrinkle-removing and anti-aging essence, so that the wrinkle-removing and anti-aging essence has a more obvious anti-aging effect.

The wrinkle-removing and anti-aging essence is prepared by the following steps:

(3-1) mixing hot spring water with water, adding natural whitening substances, humectant, radix Rhodiolae extract, radix Acanthopanacis Senticosi extract and herba Sidae Rhombifoliae extract, simultaneously adding vitamin C and skin penetrating agent, and adding buffer to obtain mixture III;

(3-2) dissolving saxifrage extract and trehalose in glycerol, adding nanogold sol, stirring, and adding vitamin E/vitamin A, skin penetrating agent and cutin softener to obtain mixture IV;

(3-3) adding the mixture III dropwise to the mixture IV with continuous stirring.

The application method of the wrinkle-removing and anti-aging essence comprises the steps of cleaning the face, and then coating a proper amount of essence on the surface of the skin.

According to a fourth aspect of the invention, there is provided a wrinkle-removing and anti-aging mask, characterized in that the mask comprises a mask carrier and a wrinkle-removing and anti-aging essence adsorbed thereon, wherein,

the mask carrier comprises artificial fiber cloth, natural fiber cloth and blended cloth;

the wrinkle-removing and anti-aging essence is prepared by the method of the third aspect.

The wrinkle-removing and anti-aging mask is prepared by soaking a mask carrier in the wrinkle-removing and anti-aging essence, and preferably soaking for more than 4 hours.

The application method of the wrinkle-removing and anti-aging mask comprises the steps of cleaning the face, covering the mask on the surface of the skin, and preferably covering for 15-20 minutes.

The essence and the mask containing the nanogold and the hot spring water provided by the invention have the following beneficial effects:

(1) the artificial and chemical addition is not really realized, the green moisturizing and beauty maintaining effects are realized, and the sealing is completely realized without preservative, bactericide, petroleum surfactant, spice and mineral oil;

(2) the skin-care product has small irritation to skin, does not contain chemical agents such as Diethanolamine (DEA), Monoethanolamine (MEA), Triethanolamine (TEA), video Pigments (FD & C Color Pigments), essence (Fragrance), polyquaternium-15 (Quaternium-15), Triclosan (Triclosan), minerals and the like, which can block, irritate, poison and even have potential carcinogenic threat to human body and skin, and prevents the skin from being damaged by artificial synthetic substances from the source;

(3) the whitening effect of the whitening and skin balancing essence is remarkable;

(4) the wrinkle-removing anti-aging essence has obvious effects of removing aging and removing wrinkles;

(5) the preparation method of the essence and the facial mask is simple.

Examples

Preparing materials:

the hot spring water used in the following examples is Nanjing Tangshan hot spring water, which comprises the following components in parts by weight:

the method is characterized in that the nano-gold is prepared by the method described in 'Nanojin preparation and application in cosmetics' 2010(5)12-17, specifically, 10mL of chloroauric acid solution and 80mL of triple distilled water are added into a beaker, the beaker is placed on a digital display speed measurement constant temperature magnetic stirrer, the beaker is heated and stirred at the rotating speed of 600r/min, the temperature is increased to 90 ℃, the constant temperature is kept for 2min, and a certain 1mL of reducing agent Na is removed by a pipette₃C₆H₅O₇Rapidly adding the solution into the mixed solution at one time, starting timing to ensure that the color of the liquid is constant, continuously heating for 9min, stopping heating, continuously stirring for 5min, stopping stirring, and cooling to room temperature to obtain nano gold sol, wherein the nano gold is 8% by weight of the nano gold based on the total weight of the nano gold sol, and the particle size of the prepared nano gold is 10-20 nm;

native methyl amino acid proline was purchased from: wuhan Dongkong science and technology Limited;

the method for extracting a ginseng extract was the extraction method described in reference to CN102961423B, and specifically,

(1) primary extraction: pulverizing dry Ginseng radix, adding into multifunctional hot reflux extraction tank, adding 75% ethanol 5 times of Ginseng radix, extracting for 2 hr, removing primary extractive solution, and leaving primary Ginseng radix residue;

(2) secondary extraction: adding 85% ethanol 4 times of the weight of Ginseng radix into the primary Ginseng radix residue, extracting for 2.5 hr, removing the secondary extractive solution, and leaving the secondary Ginseng radix residue;

(3) and (3) extracting for three times: adding 95% ethanol 4 times of the weight of Ginseng radix into the secondary Ginseng radix residue, extracting for 2 hr, removing the third extractive solution, and leaving the third Ginseng radix residue;

(4) mixing the extractive solutions, and ultrafiltering: mixing the extractive solutions, standing at room temperature for 48 hr, collecting supernatant, filtering with filter cloth, ultrafiltering with ceramic membrane, and collecting ultrafiltrate;

(5) ADS-4 resin: passing the ultrafiltrate through ADS-4 resin, and collecting effluent;

(6) concentrating and drying: concentrating the eluate to obtain paste, drying, and pulverizing to obtain Ginseng radix extract without pesticide residue;

in this example, the ginseng is a dried root of ginseng belonging to the genus Panax of the family Araliaceae, wherein the ginseng extract contains ginsenoside A + B10mg/L based on the total weight of the ginseng extract;

the extraction method of the wolfberry extract is the extraction method described with reference to CN1263108A, and specifically, the wolfberry is separated and wolfberry seeds are removed, the obtained wolfberry stock solution is subjected to bale filtration by using 500-mesh polyester filter cloth, is separated by using a 50-ten-thousand molecular weight membrane, then wolfberry polysaccharide is entrapped by using 1-10-ten-thousand molecular weight membrane, and is subjected to column chromatography after vacuum freeze drying, and the eluent is double distilled water, the wolfberry used in the embodiment is mature fruit of Ningxia wolfberry belonging to the solanaceae family, wherein the wolfberry comprises the following components in parts by weight based on the total weight of the wolfberry extract:

10g/mL of lycium barbarum polysaccharide;

8g/mL of alanine;

the extraction method of the seaweed extract was the extraction method described in reference to CN104069059A, and specifically,

(1) pulverizing seaweed into 40 mesh coarse powder with a pulverizer;

(2) adding 50% ethanol 10 times of the coarse powder, grinding into 650 mesh fine powder with a colloid mill, and circularly extracting in the colloid mill to obtain extractive solution;

(3) stirring the extracting solution by using 3 wt% of diatomite and 2 wt% of 300-mesh active carbon, and settling;

(4) centrifuging and separating the supernatant;

(5) adding algal polysaccharide crystal at 4 ℃, freezing and then thawing;

(6) filtering after melting, and collecting filter residue;

(7) re-dissolving the filter residue with water to obtain the seaweed extract.

The seaweed used in this example is the whole plant of sargassum fusiforme or sargassum pallidum of sargassum family, wherein the seaweed comprises 0.02 wt% -0.05 wt% of alginic acid based on the total weight of the seaweed extract;

the extraction method of the geranium extract refers to the extraction method described in CN1191262C, and specifically comprises extracting geranium with 50% alcohol, concentrating, passing through a D101 macroporous resin column, and eluting with 70% ethanol;

the geranium wilfordii used in this example is a whole herb of a plant of the family sophora geraniaceae, such as a geranium wilfordii seedling or geranium wilfordii with fruits, wherein the geranium wilfordii extract contains 12 wt% of flavones based on the total weight of the geranium wilfordii extract;

the extraction method of the rhodiola rosea extract refers to the method in CN103814954A, and specifically comprises the steps of grinding and crushing dry roots of rhodiola rosea in liquid nitrogen, adding 95% ethanol water solution for extraction, collecting the extract, and removing ethanol in the extract by concentration under reduced pressure to obtain an extract, wherein the extract contains 2 wt% of salidroside based on the total weight of the rhodiola rosea extract;

the extraction method of radix Acanthopanacis Senticosi extractive solution refers to the method described in CN102600221A, specifically, weighing 1000g of root of radix Acanthopanacis Senticosi of Araliaceae, placing into 10L round-bottom flask, soaking with 8000mL of 60% ethanol overnight, heating with electric heating jacket to slightly boil, and reflux extracting for 2 hr; repeating the steps for three times, combining the extracting solutions, and concentrating the extracting solutions to obtain an extract, wherein the extract contains eleutheroside B + E0.15wt% based on the total weight of the eleutheroside;

referring to the method described in CN101413020A, calendula officinalis flower of calendula officinalis of calendula of Compositae is soaked in anhydrous ethanol at 50 deg.C for 5 hr, the solvent is removed, and n-hexane is used for extraction under the conditions: at a pressure of 1MPa, for a period of 2 hours and at a temperature of 50 ℃, and finally removing the solvent, wherein the calendula extract comprises the following components in weight ratio:

0.02 wt% of lutein

Zeaxanthin 0.05 wt%;

the extraction method of saxifrage extract refers to the method described in basic medical journal of Chinese medicine 2005, 12(11), and specifically mainly comprises decocting leaf of saxifrage of Saxifragaceae in water, precipitating with ethanol, concentrating, extracting with ethyl acetate, concentrating the ethyl acetate extractive solution, and cooling, wherein the total weight of the saxifrage extract is calculated by the sum of the weight of bergenin, quercitrin, quercetin and gallic acid, and the extract contains bergenin, quercitrin, quercetin and gallic acid in an amount of 3 wt%;

DMEM, available from Hyclone, model SH30022.01B;

FBS, available from Gibco corporation, model 16000-044;

a 1% penicillin streptomycin double resistant solution, available from Gibco, model 15140-122;

0.05%/Trypsin/EDTA solution, available from Gibco, model 25300-054;

PBS, model SH30256.01B from Hyclone.

Example 1 preparation of whitening and skin-balancing essence

(1) Dissolving ferulic acid in the aqueous solution of hot spring water at 60 ℃ under 1.5 atmospheric pressure, adding butanediol, adding the rhodiola rosea extract, the acanthopanax senticosus extract, the calendula officinalis extract, the saxifraga stolonifera extract and the vitamin C which are described in the material preparation after full dissolution, finally adding sodium dihydrogen phosphate, adjusting the pH of a water phase to be about 7, and uniformly mixing water phase substances to prepare a mixture 1;

(2) at 60 ℃ and 1.0 atmospheric pressure, uniformly mixing trehalose and the oil-phase substances according to the following parts by weight to obtain a mixture 2;

(3) and (3) dropwise adding the mixture 1 into the mixture 2 at 60 ℃, and continuously stirring for 50min to prepare the whitening and skin balancing essence.

TABLE 1 weight of each component (total 100g)

Example 2 preparation of whitening and skin-balancing essence

This example operates the same as example 1 except that the weight of the ingredients used are as shown in table 2 below.

TABLE 2 weight of each component (total 100g)

Example 3 preparation of a whitening and skin-balancing mask

And (3) soaking the non-woven fabric mask carrier in the whitening and skin balancing essence prepared in the example 1 for 4 hours to prepare the whitening and skin balancing mask.

Example 4 preparation of wrinkle-removing and anti-aging essence

(1) Dissolving ferulic acid in an aqueous solution of hot spring water at 60 ℃ under 1.5 atmospheric pressure, adding butanediol, fully dissolving, then adding the ginseng extract, the medlar extract, the geranium extract, the seaweed extract and the vitamin C which are described in the material preparation, finally adding sodium dihydrogen phosphate, adjusting the pH of an aqueous phase to be about 7, and uniformly mixing aqueous phase substances to prepare a mixture 1;

(2) at 60 ℃ and 1.0 atmospheric pressure, uniformly mixing trehalose and the oil-phase substances according to the following parts by weight to obtain a mixture 2;

(3) and (3) dropwise adding the mixture 1 into the mixture 2 at 60 ℃, and continuously stirring for 50min to prepare the wrinkle-removing and anti-aging essence.

TABLE 3 amounts of ingredients (total 100g)

Example 5 preparation of wrinkle-removing and anti-aging essence

This example operates the same as example 1 except that the weights of the ingredients used are as shown in table 4 below.

TABLE 4 dosage of each component (total 100g)

Example 6 preparation of wrinkle-removing and anti-aging mask

And (3) soaking the non-woven fabric mask carrier in the wrinkle-removing and anti-aging essence prepared in the embodiment 3 for 4 hours to prepare the wrinkle-removing and anti-aging mask.

Comparative example

Comparative example 1

This comparative example is the same as example 1 except that no nanogold sol was added.

Comparative example 2

This comparative example is the same as example 1 except that no hot spring water from south Beijing Tangshan of China was added.

Comparative example 3

This comparative example is the same as example 1 except that neither nanogold nor hot spring water from south china kyo soup was added.

Comparative example 4

This comparative example is the same as example 4 except that no nanogold sol was added.

Comparative example 5

This comparative example is the same as example 4 except that no hot spring water from south Beijing Tangshan of China was added.

Comparative example 6

This comparative example is the same as example 4 except that neither nanogold nor hot spring water from south china kyo soup was added.

Examples of the experiments

Experimental example 1 determination of skin toxicity of Hot spring Water of Nanjing Tangshan

The Real-Time label-free cell analysis technology (RTCA Real Time cellular analysis) is adopted for the hot spring water of Nanjing Tangshan, and the RTCA system is used for detecting the influence of different concentrations of metasilicic acid in the mineral-rich hot spring water on the growth of Hs27 (skin fibroblast), KB (human oral epithelial cell) and Hela (cervical carcinoma cell).

And (3) experimental operation:

sucking out the old culture solution in the culture dish in an ultra-clean workbench under the aseptic condition; washing with PBS for 1 time, adding 0.5ml (T25 culture bottle) trypsin solution containing EDTA into the culture dish, covering, incubating in 37 deg.C incubator for 2-6min, and terminating digestion; adding the culture medium into a 4 ml/culture flask to form cell suspension, uniformly blowing the cells by using a pipette, and counting the concentration of the cell suspension by using a counting plate to prepare the cell concentration required by the experiment: 50000 cells/ml.

Experimental method-the use of RTCA system to detect the effect of hot spring water on cell growth:

add 50. mu.l of medium to the wells of E-Plate 96;

place E-Plate96 on RTCAStation;

the RTCA system automatically scans and detects;

taking out the E-Plate96, and adding 100 μ l of the uniformly mixed cell suspension into each well to make the number of cells in each well be 5 k/well; (Note: after addition of E-Plate96 to the cells, it is not necessary to mix the cells with the original medium in the well)

Placing E-Plate96 in a clean bench at room temperature for 30 min;

E-Plate96 was placed on the RTCASTATION in the incubator;

after the system automatically scans, real-time dynamic detection of cell proliferation is carried out;

the cells were treated with the drug at about 24h of growth, i.e., in hot spring water at a concentration gradient of 100. mu.g/ml, 50. mu.g/ml, 25. mu.g/ml, 12.5. mu.g/ml, 6.25. mu.g/ml, 3.12. mu.g/ml, 1.562. mu.g/ml metasilicate.

From the above experiments, it was confirmed that the hot spring water containing metasilicic acid at a high concentration of 25g/ml or more shows a weak effect of inhibiting the growth of cells in three, and that the hot spring water hardly has toxicity to the skin.

Experimental example 2 evaluation of radical scavenging Performance of mask by DPPH method

DPPH is a relatively stable lipid radical, and the ethanol solution of DPPH is purple and has a maximum absorption peak at 517 nm. After the antioxidant is added, DPPH captures an electron to be paired with a free electron, fades purple to be a colorless substance, the absorption at 517nm disappears, and the fading degree is in quantitative relation with the number of the accepted electrons.

The change of absorbance (Abs) after DPPH free radicals react with the sample solution was measured by an ultraviolet-visible spectrophotometer, and the ability of the sample to provide hydrogen atoms and scavenge free radicals against oxidation was determined, with the results shown in table 1 below.

The antioxidant of the vitamin C with different known standard concentrations is measured by DPPH spectrophotometry by taking an antioxidant vitamin C as a reference system, and the antioxidant is obtained through research, along with the increase of the concentration of the vitamin C, the amount of the antioxidant in the thermal spring water mask is increased, the capability of removing DPPH free radicals is enhanced, the intensity of an absorption peak of DPPH at 517nm is reduced, namely, the larger the change rate of absorbance value is, the stronger the capability of removing free radicals for resisting oxidation is.

Table 1 results for radical scavenging Performance of the samples

Test specimen	Absorbance change rate (%)
		Example 1	7
Example 2	8
		Example 4	10
Example 5	9
		Comparative example 1	3
Comparative example 2	2
		Comparative example 3	1
Comparative example 4	4
		Comparative example 5	3
Comparative example 6	2.5

Experimental example 3 evaluation of whitening efficacy of whitening and skin-weighing essence (tyrosinase activity inhibition experiment)

The samples used in this example were those obtained in examples 1 to 4 and comparative examples 1 to 4.

The whitening effect of the whitening and skin-balancing essence is measured by adopting a tyrosinase activity method.

The method used in the present invention is described in "Biochemical enzymology assay for tyrosinase Activity in cells", daily chemical industry ", 2001 (4): 52-53, i.e., the stronger the inhibitive ability, the more remarkable the whitening effect.

The test results were as follows:

TABLE 2 tyrosinase activity inhibition rate of the samples

Test specimen

Tyrosinase activity inhibition ratio (%)

Example 1	50
		Example 2	48
Example 4	40
		Example 5	42
Comparative example 1	28
		Comparative example 2	25
Comparative example 3	22
		Comparative example 4	20
Comparative example 5	21
		Comparative example 6	18

Experimental example 4 whitening and skin-weighing essence whitening efficacy evaluation (animal skin tissue test)

The sample used in this example was the sample prepared in example 1.

The specific operation method comprises the following steps:

(1) common grade healthy adult brown-yellow Hartley guinea pigs, female, 40, body weights (250 + -20) g, purchased from the Guangdong province medical animal center.

(2) Randomly selecting 10 guinea pigs, making model, collecting brown skin part, removing coat, irradiating with UVB ultraviolet ray at wavelength of 310nm, and accumulating total irradiation amount of 5000mJ/cm²The irradiation is carried out 1 time per day for 1h for 7 days, and UVB ultraviolet irradiation is carried out every day.

Covering the non-irradiated part with a spot tester, detecting MI and EI indexes of the skin at regular time, stopping irradiation for 7d, continuously dripping 2 ml/piece of original whitening skin-balancing mask liquid every day, and euthanizing the animal to take skin tissues.

(3) Selecting 10 guinea pigs at random, making model, removing coat from brown skin part, irradiating with UVB ultraviolet ray at wavelength of 310nm, and accumulating total irradiation amount of 5000mJ/cm²Irradiating for 1 time every day, 1h every time, continuing for 7d, dripping 2 ml/piece of original whitening skin-balancing facial mask solution on an irradiated part after UVB ultraviolet irradiation experiment every day, and euthanizing animals to take skin tissues.

(4)20 animals were randomly divided into 2 groups, 10 whitening and skin balancing mask stock solution groups, and 10 blank control groups. A whitening and skin balancing facial mask stock solution group is characterized in that 4 relatively independent areas on the back of each guinea pig are taken as administration areas, a closed spot tester is taken, about (0.8 +/-0.2) mL of a test object is placed in the spot tester and is in direct contact with the skin, the spot tester is fixed for 6h, and after the test is finished, the residual test object is removed by warm water.

Model control areas were each smeared with 1mL of 0.9% saline for 5 weeks and skin tissue was harvested after euthanasia.

(5) The obtained skin tissue is fixed by 10% paraformaldehyde solution, and is sliced by conventional paraffin, and the following histochemical staining is respectively carried out:

and L-dopa staining, namely dewaxing the section, soaking the section in 0.1 percent L-dopa solution at 37 ℃ for 4 hours, observing staining change under a mirror, washing the section by PBS, washing the section by distilled water, and performing HE counterstaining.

Fontana-Masson silver ammonia staining, namely dewaxing the slices, washing the slices with water, placing the slices in a silver ammonia solution, placing the slices in a water bath at 60 ℃ for 60min, staining the slices with 0.1% gold chloride for 10min, washing the slices with deionized water, and then using 5% Na₂SO₃Soaking in the solution for 5min, washing with tap water and deionized water, respectively, dyeing with 0.2% nuclear fast red for 5min, and washing with deionized waterAnd (6) washing. Conventional dehydration, xylene clarity and neutral gum blocking.

(6) Microscopic photographing is carried out on the stained histological sections, the Image is analyzed by Image-ProPlus6.0 analysis software, the results of skin color measurement, dopa staining and ammonia silver staining before and after ultraviolet irradiation are shown in the following table 3,

TABLE 3

(7) The experimental results show that after the brown skin is irradiated by UVB, MI is obviously increased, and EI index is obviously reduced. Histochemical staining showed that the melanin content was all significantly increased, and the melanin granule staining was graded close to grade 5. The values of L/M, L/K and L/E were significantly different from those before and after irradiation.

Indicating that the total amount of the additive is 5000mJ/cm²The skin of the guinea pig can be blackened by the irradiation of the left and right UVB, and after the original solution of the whitening and skin balancing facial mask is dripped, the MI value is obviously lower than that of an unused control group, and the EI value is relatively high; after the skin tissue of guinea pigs in the non-irradiated group is dripped with the original whitening and skin balancing mask solution, the MI value is reduced, but the change value is smaller than the change value range after UVB ultraviolet irradiation.

(8) The experimental result shows that the whitening and skin balancing mask has a whitening effect on animal epidermal tissues, and the whitening and repairing effect after being damaged by ultraviolet irradiation is more obvious.

EXAMPLE 5 evaluation of anti-aging efficacy of wrinkle-removing anti-aging mask (human skin test)

The standard used in this example was the sample prepared in example 4.

(1) Selecting 24 subjects to participate in a product efficacy test, wherein the females with the age of 25-70 have no serious system diseases, immunodeficiency or autoimmune diseases; an inactive allergic disease; the past has no history of allergy to skin care cosmetics; hormone drugs and immunosuppressive agents have not been used in the last month; those who did not participate in other clinical trials; the volunteers participate in and can complete the specified contents according to the test requirements.

(2) The test part is the random left and right faces of the face, and the product is required to completely cover one side of the face of the subject for 15 minutes; randomly arranging the left and right faces for a control test using a wrinkle-removing anti-aging and standard (a product known to have some anti-wrinkle effect, provided by dr. schrader research institute, germany) respectively; the subjects used the mask once on three days for a period of 4 weeks.

(3) The results of the two areas were compared by inserting 1 set of standards and blank tests during all product tests, using the standards on one test area and no product on the other side as a blank.

(4) Statistical analysis was performed using statistica7.1(Statsoft Company, USA) software

First, a descriptive statistical analysis was performed on the initial and final FDD values for all subjects and test areas, and a non-parametric test method was used for statistical analysis of FDD values.

(5) According to the analysis result of wrinkle FDD, fine structures were increased and significantly varied in the frequency of wrinkle depth distribution FDD, and facial wrinkles were reduced in 24 subjects.

The invention has been described in detail with reference to specific embodiments and illustrative examples, but the description is not intended to be construed in a limiting sense. Those skilled in the art will appreciate that various equivalent substitutions, modifications or improvements may be made to the technical solution of the present invention and its embodiments without departing from the spirit and scope of the present invention, which fall within the scope of the present invention. The scope of the invention is defined by the appended claims.

Claims (14)

1. The whitening and skin balancing essence is characterized by comprising the following components in parts by weight based on the total weight of the essence:

the particle size of the nano gold in the nano gold sol is 1-100 nm, and the weight fraction of the nano gold is 0.0001-10% based on the total weight of the nano gold sol.

2. The whitening and skin balancing essence of claim 1, wherein the nano gold in the nano gold sol has a particle size of 5-50 nm, and the nano gold accounts for 0.01-8 wt% of the total weight of the nano gold sol.

3. The whitening and skin balancing essence of claim 1, further comprising the following components in parts by weight, based on the total weight of the essence:

4. the whitening and skin balancing essence of claim 3, wherein the hot spring water comprises the following components in concentration:

the iron sulfate,

the hot spring water is the hot spring water of the Nanjing Tangshan of China; and/or

The raw material medicine of the rhodiola rosea extracting solution is dry roots and rhizomes of rhodiola crenulata of Crassulaceae; and/or

The raw material medicine of the acanthopanax root extracting solution is the root and the rhizome of acanthopanax root of the plant of the acanthopanax of the araliaceae; and/or

The raw material medicine of the calendula extract is the flower of calendula of Compositae; and/or

The herba Saxifragae extract is prepared from leaf of Saxifraga stolonifera of Saxifragaceae; and/or

The bioactive substance is selected from hyaluronic acid and trehalose; and/or

The natural whitening substance is selected from tranexamic acid and ferulic acid; and/or

The vitamins are selected from vitamin A, vitamin C and vitamin E; and/or

The humectant is selected from natural proline methyl amine, butanediol, polyglutamic acid, hyaluronic acid, sodium pyrrolidone carboxylate, urea and lactic acid; and/or

The skin penetrant is selected from decyl methyl sulfoxide (DCMS), azone, 2-pyrrolidone, 5-methyl-2-pyrrolidone, 1, 5-dimethyl-2-pyrrolidone, oleic acid, isopropyl myristate, propylene glycol dinonyl acid ester and diethyl sebacate; and/or

The buffer is selected from sodium dihydrogen phosphate, disodium hydrogen phosphate, potassium dihydrogen phosphate and dipotassium hydrogen phosphate; and/or

The cutin softening agent is selected from salicylic acid, coal tar and propylene glycol; and/or

The antiseptic is methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, n-butyl p-hydroxybenzoate and isobutyl p-hydroxybenzoate.

5. The whitening and skin balancing essence of claim 4,

the bioactive substance is trehalose; and/or

The natural whitening substance is ferulic acid; and/or

The vitamin is selected from vitamin C and vitamin E; and/or

The humectant is selected from natural methyl proline, butanediol and polyglutamic acid; and/or

The skin penetrant is selected from azone, 2-pyrrolidone, 5-methyl-2-pyrrolidone and 1, 5-dimethyl-2-pyrrolidone; and/or

The buffer is sodium dihydrogen phosphate; and/or

The cutin softener is propylene glycol; and/or

The preservative is methyl p-hydroxybenzoate.

6. The whitening and skin balancing essence according to one of claims 1 to 5,

the rhodiola rosea extracting solution is based on the total weight of the rhodiola rosea extracting solution, and the rhodiola rosea extracting solution contains 0.5-2 wt% of rhodiola rosea glycoside;

the acanthopanax root extracting solution is based on the total weight of the acanthopanax root extracting solution, and comprises 0.05 wt% -0.15 wt% of eleutheroside B and E;

the calendula extract is based on the total weight of the calendula extract and comprises the following components in parts by weight:

0.01 to 0.03 weight percent of lutein

0.02 wt% -0.10 wt% of zeaxanthin;

the saxifrage extract comprises 1-5 wt% of bergenin, quercitrin, quercetin and gallic acid based on the total weight of the saxifrage extract and the sum of the weight of the bergenin, the weight of the quercitrin, the weight of the quercetin and the weight of the gallic acid.

7. The whitening and skin balancing mask is characterized by comprising a mask carrier and whitening and skin balancing essence adsorbed on the mask carrier, wherein,

the mask carrier is selected from artificial fiber cloth, natural fiber cloth and blended cloth;

the whitening and skin balancing essence is as defined in any one of claims 1-6.

8. The wrinkle-removing and anti-aging essence is characterized by comprising the following components in parts by weight based on the total weight of the essence:

the particle size of the nano gold in the nano gold sol is 1-100 nm, and the weight fraction of the nano gold is 0.0001-10% based on the total weight of the nano gold sol.

9. The wrinkle-removing and anti-aging essence according to claim 8, wherein the nano-gold in the nano-gold sol has a particle size of 5-50 nm, and the weight fraction of nano-gold is 0.01-8% based on the total weight of the nano-gold sol.

10. The wrinkle-removing and anti-aging essence according to claim 8, further comprising the following components in parts by weight, based on the total weight of the essence:

11. the wrinkle-removing and anti-aging essence according to claim 10,

the hot spring water comprises the following components in concentration:

the iron sulfate,

the hot spring is hot spring water of Nanjing Tangshan of China; and/or

The raw material medicine of the ginseng extract is dried root of ginseng of Panax of Araliaceae; and/or

The raw material medicine of the medlar extracting solution is mature fruits of Ningxia medlar which is a solanaceae plant; and/or

The herba Erodii seu Geranii is whole plant with fruit such as Geranium robustum or Geranium thunbergii, Geranium nepalense, Geranium sibiricum, Geranium wilfordii, Geranium tuberosum, etc. of Geranium robustum of family Bodinamiaceae; and/or

The raw material medicine of the seaweed extract is the whole plant of sargassum fusiforme or sargassum pallidum of sargassum family; and/or

The bioactive substance is selected from hyaluronic acid and trehalose; and/or

The natural whitening substance is selected from tranexamic acid and ferulic acid; and/or

The vitamins are selected from vitamin A, vitamin C and vitamin E; and/or

The humectant is selected from natural proline methyl amine, butanediol, polyglutamic acid, hyaluronic acid, sodium pyrrolidone carboxylate, urea and lactic acid; and/or

The skin penetrant is selected from decyl methyl sulfoxide (DCMS), azone, 2-pyrrolidone, 5-methyl-2-pyrrolidone, 1, 5-dimethyl-2-pyrrolidone, oleic acid, isopropyl myristate, propylene glycol dinonyl acid ester and diethyl sebacate; and/or

The buffer is selected from sodium dihydrogen phosphate, disodium hydrogen phosphate, potassium dihydrogen phosphate and dipotassium hydrogen phosphate; and/or

The cutin softening agent is selected from salicylic acid, coal tar and propylene glycol; and/or

The antiseptic is methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, propyl p-hydroxybenzoate, n-butyl p-hydroxybenzoate and isobutyl p-hydroxybenzoate.

12. The wrinkle-removing and anti-aging essence according to claim 11,

the bioactive substance is trehalose; and/or

The natural whitening substance is ferulic acid; and/or

The vitamin is selected from vitamin C and vitamin E; and/or

The humectant is selected from natural methyl proline, butanediol and polyglutamic acid; and/or

The skin penetrant is selected from azone, 2-pyrrolidone, 5-methyl-2-pyrrolidone and 1, 5-dimethyl-2-pyrrolidone; and/or

The buffer is sodium dihydrogen phosphate; and/or

The cutin softener is propylene glycol; and/or

The preservative is methyl p-hydroxybenzoate.

13. The wrinkle-removing and antiaging essence as claimed in one of claims 8 to 12, wherein,

the ginseng extracting solution is based on the total weight of the ginseng extracting solution, and the ginseng extracting solution contains 5-15 mg/L of ginsenoside A and ginsenoside B;

the wolfberry fruit extracting solution is based on the total weight of the wolfberry fruit extracting solution, and comprises the following components in concentration:

5-10 g/mL of lycium barbarum polysaccharide;

5-10 g/mL of alanine;

the geranium extract comprises 10-15 wt% of flavone based on the total weight of the geranium extract;

the seaweed extract comprises 0.02 wt% -0.05 wt% of alginic acid based on the total weight of the seaweed extract.

14. The wrinkle-removing and anti-aging mask is characterized by comprising a mask carrier and wrinkle-removing and anti-aging essence adsorbed on the mask carrier, wherein,

the facial mask carrier is selected from artificial fiber cloth, natural fiber cloth and blended cloth;

the wrinkle-removing and anti-aging essence is as defined in any one of claims 8 to 13.