CN105557517A - Method for culturing ginkgo seedlings by culture medium - Google Patents
Method for culturing ginkgo seedlings by culture medium Download PDFInfo
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- CN105557517A CN105557517A CN201510924770.XA CN201510924770A CN105557517A CN 105557517 A CN105557517 A CN 105557517A CN 201510924770 A CN201510924770 A CN 201510924770A CN 105557517 A CN105557517 A CN 105557517A
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- ginkgo
- seedling
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- ginkgo seedling
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention provides a method for culturing ginkgo seedlings by a culture medium, and relates to the technical field of ginkgo planting. The method comprises the following steps and processes of culture medium preparation, seedling cleaning, drying, soaking and culturing. The ginkgo seedlings cultured by using the technology provided by the invention have high planting survival rate during the later-stage planting, can adapt to various kinds of soil of different properties, and have low requirements on the land; the management is easy after the planting; only the simple normal maintenance is needed; special management is not needed.
Description
Technical field:
The present invention relates to ginkgo planting technology field, be specifically related to a kind of method of medium culture ginkgo seedling.
Background technology:
Ginkgo is Ginkgoaceae, Ginkgo deciduous tree.Before ginkgo appears at several hundred million years, be Relict Plant the most ancient in the gymnosperm that carries over after quaternary glacier motion, the alive ginkgo of existing work is rare and disperse, and upper veteran at the age of one hundred years old is rare, all become extinct, so ginkgo has again the laudatory title of living fossil with its every other plant with guiding principle.Mutation and kind have: yellow leaf ginkgo, tower-like ginkgo, baiera, weeping branch ginkgo, variegated leaf ginkgo etc. 26 kinds.
The fruit of ginkgo is commonly called as gingko, and therefore ginkgo has another name called maidenhair tree.Ginkgo growth is comparatively slow, and the life-span is extremely long, ties ginkgo nut and wants for two more than ten years, ability large result after 40 years under natural conditions from plant to, therefore having again people that it is called " Gong Sunshu ", have the implication of " public affairs are planted and grandson must eat ", is the venerable old man or lady in tree, have and view and admire, economical, medical value.
When ginkgo carries out plantation and seedling fostering, often output is not high, and seedling survival rate after plantation of cultivating is not high, and higher to the requirement in soil, is unfavorable for extensive popularizing planting.
Summary of the invention:
It is high, lower to the requirement in soil that technical problem to be solved by this invention is to provide a kind of survival rate of planting, and is easy to the method for the use medium culture ginkgo seedling managed after plantation.
Technical problem to be solved by this invention adopts following technical scheme to realize:
A method for medium culture ginkgo seedling, comprises following methods step:
(1) medium preparing:
Ginkgo leaf juicing 400-450 part
Ginkgo is with powder 800-900 part
Manganese sulphate 22-25 part
Zinc sulphate 10-15 part
Boric acid 5-8 part
Inositol 80-120 part
Sulfuric acid thiamine element 40-70 part
Nicotinic acid 1-1.5 part
Glycine 3-4 part
Sodium p-nitrophenoxide 5-10 part
Sodium onitrophenol 4-5 part
Citric acid 40-50 part
Mentioned component is put in culture dish according to this and stirs, then 24 hours are left standstill under being placed on the low temperature environment of 0-3 DEG C, allow between each composition and mutually ooze conjunction, leave standstill wild Oryza species preparation to terminate, use ginkgo leaf extracting juice and ginkgo can increase the nutritive element of ginkgo in medium with powder in the medium, use it to carry out ginkgo cultivating the growing ability that can increase ginkgo seedling, promote the growth of root system, increase the adaptability to soil;
(2) cleaned up by the root of ginkgo seedling, and soak 1-2 hour with the physiological saline that concentration is 40%, the ginkgo seedling after immersion can promote that root is to the absorption of medium;
(3) after having soaked, ginkgo seedling is placed in warm air drier and dries 10-15 minute by the temperature of 20-25 degree Celsius, oven dry can make the moisture of tiny root discharge fast, infiltration rate to liquid nutrient can be increased entering into next link, promote the absorption of nutrient;
(4) ginkgo seedling single is placed on diameter after terminating by oven dry is in the plastic bottle of 5-8 centimetre, then the ginkgo resin injecting 400 milliliters in plastic bottle extracts juice, and put the cool place place placement 1-2 days being neatly placed into ventilation, ginkgo seedling can be made like this before cultivating can fully to absorb nutrient of the same clan, ensure that the growth of seedling and drawing of nutrient;
(5) ready medium being poured into the degree of depth is in the cement pit of 10-20 centimetre, and the medium degree of depth is that 10-13 centimetre is dark, is then taken out by the ginkgo seedling in above-mentioned plastic bottle and evenly puts in cement pit;
(6) putting is evenly the plastic greenhouse of 1 meter at the top antenna height of cement pit, and ensures the sealability of plastic greenhouse;
(7) then constant temperature setting is carried out to plastic greenhouse, ensure that temperature of shed remains between 35-45 degree Celsius, the setting of constant temperature can ensure that the speed of seedling absorption medium is more stable, can not absorb not smooth problem, and the temperature of regulation is also carry out setting according to the characteristic of ginkgo;
(8) cultivate after 25-35 days and terminate to cultivate, ginkgo seedling can be taken out and carry out there is soil cultivation;
The invention has the beneficial effects as follows: the ginkgo seedling using the method to cultivate plants survival rate when carrying out later stage plantation high, and various soil of different nature, lower to the requirement in soil can be adapted to, be easy to management after plantation and only simply need carry out conventional maintenance, without the need to special management.
Embodiment:
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with concrete example, setting forth the present invention further.
Specific embodiment 1:
A method for medium culture ginkgo seedling, comprises following methods step:
(1) medium preparing:
Ginkgo leaf juicing 450 parts
Ginkgo is with 900 parts, powder
Manganese sulphate 25 parts
15 parts, zinc sulphate
Boric acid 8 parts
Inositol 120 parts
Sulfuric acid thiamine element 70 parts
1.5 parts, nicotinic acid
Glycine 4 parts
Sodium p-nitrophenoxide 10 parts
Sodium onitrophenol 5 parts
Citric acid 50 parts
Mentioned component is put in culture dish according to this and stirs, then 24 hours are left standstill under being placed on the low temperature environment of 3 DEG C, allow between each composition and mutually ooze conjunction, leave standstill wild Oryza species preparation to terminate, use ginkgo leaf extracting juice and ginkgo can increase the nutritive element of ginkgo in medium with powder in the medium, use it to carry out ginkgo cultivating the growing ability that can increase ginkgo seedling, promote the growth of root system, increase the adaptability to soil;
(2) cleaned up by the root of ginkgo seedling, and soak 2 hours with the physiological saline that concentration is 40%, the ginkgo seedling after immersion can promote that root is to the absorption of medium;
(3) after having soaked, ginkgo seedling is placed in warm air drier and dries 15 minutes by the temperature of 25 degrees Celsius, oven dry can make the moisture of tiny root discharge fast, infiltration rate to liquid nutrient can be increased entering into next link, promote the absorption of nutrient;
(4) ginkgo seedling single is placed on diameter after terminating by oven dry is in the plastic bottle of 8 centimetres, then the ginkgo resin injecting 400 milliliters in plastic bottle extracts juice, and put the cool place place placement 2 days being neatly placed into ventilation, ginkgo seedling can be made like this before cultivating can fully to absorb nutrient of the same clan, ensure that the growth of seedling and drawing of nutrient;
(5) ready medium being poured into the degree of depth is in the cement pit of 10-20 centimetre, and the medium degree of depth is that 10-13 centimetre is dark, is then taken out by the ginkgo seedling in above-mentioned plastic bottle and evenly puts in cement pit;
(6) putting is evenly the plastic greenhouse of 1 meter at the top antenna height of cement pit, and ensures the sealability of plastic greenhouse;
(7) then constant temperature setting is carried out to plastic greenhouse, ensure that temperature of shed remains between 45 degrees Celsius, the setting of constant temperature can ensure that the speed of seedling absorption medium is more stable, can not absorb not smooth problem, and the temperature of regulation is also carry out setting according to the characteristic of ginkgo;
(8) cultivate after 35 days and terminate to cultivate, ginkgo seedling can be taken out and carry out there is soil cultivation;
Specific embodiment 2:
A method for medium culture ginkgo seedling, comprises following methods step:
(1) medium preparing:
Ginkgo leaf juicing 400 parts
Ginkgo is with 800 parts, powder
Manganese sulphate 22 parts
10 parts, zinc sulphate
Boric acid 5 parts
Inositol 80 parts
Sulfuric acid thiamine element 40 parts
1 part, nicotinic acid
Glycine 3 parts
Sodium p-nitrophenoxide 5 parts
Sodium onitrophenol 4 parts
Citric acid 40 parts
Mentioned component is put in culture dish according to this and stirs, then 24 hours are left standstill under being placed on the low temperature environment of 0 DEG C, allow between each composition and mutually ooze conjunction, leave standstill wild Oryza species preparation to terminate, use ginkgo leaf extracting juice and ginkgo can increase the nutritive element of ginkgo in medium with powder in the medium, use it to carry out ginkgo cultivating the growing ability that can increase ginkgo seedling, promote the growth of root system, increase the adaptability to soil;
(2) cleaned up by the root of ginkgo seedling, and soak 1 hour with the physiological saline that concentration is 40%, the ginkgo seedling after immersion can promote that root is to the absorption of medium;
(3) after having soaked, ginkgo seedling is placed in warm air drier and dries 10 minutes by the temperature of 20 degrees Celsius, oven dry can make the moisture of tiny root discharge fast, infiltration rate to liquid nutrient can be increased entering into next link, promote the absorption of nutrient;
(4) ginkgo seedling single is placed on diameter after terminating by oven dry is in the plastic bottle of 5 centimetres, then the ginkgo resin injecting 400 milliliters in plastic bottle extracts juice, and put the cool place place placement 1 day being neatly placed into ventilation, ginkgo seedling can be made like this before cultivating can fully to absorb nutrient of the same clan, ensure that the growth of seedling and drawing of nutrient;
(5) ready medium being poured into the degree of depth is in the cement pit of 10 centimeters, the medium degree of depth be 10 centimeters dark, then by above-mentioned plastic bottle ginkgo seedling take out evenly put in cement pit;
(6) putting is evenly the plastic greenhouse of 1 meter at the top antenna height of cement pit, and ensures the sealability of plastic greenhouse;
(7) then constant temperature setting is carried out to plastic greenhouse, ensure that temperature of shed remains between 35 degrees Celsius, the setting of constant temperature can ensure that the speed of seedling absorption medium is more stable, can not absorb not smooth problem, and the temperature of regulation is also carry out setting according to the characteristic of ginkgo;
(8) cultivate after 25 days and terminate to cultivate, ginkgo seedling can be taken out and carry out there is soil cultivation;
Specific embodiment 3
A method for medium culture ginkgo seedling, comprises following methods step:
(1) medium preparing:
Ginkgo leaf juicing 420 parts
Ginkgo is with 850 parts, powder
Manganese sulphate 23 parts
13 parts, zinc sulphate
Boric acid 6 parts
Inositol 100 parts
Sulfuric acid thiamine element 55 parts
1.3 parts, nicotinic acid
Glycine 3.5 parts
Sodium p-nitrophenoxide 8 parts
Sodium onitrophenol 4.5 parts
Citric acid 45 parts
Mentioned component is put in culture dish according to this and stirs, then 24 hours are left standstill under being placed on the low temperature environment of 1.5 DEG C, allow between each composition and mutually ooze conjunction, leave standstill wild Oryza species preparation to terminate, use ginkgo leaf extracting juice and ginkgo can increase the nutritive element of ginkgo in medium with powder in the medium, use it to carry out ginkgo cultivating the growing ability that can increase ginkgo seedling, promote the growth of root system, increase the adaptability to soil;
(2) cleaned up by the root of ginkgo seedling, and soak 1.5 hours with the physiological saline that concentration is 40%, the ginkgo seedling after immersion can promote that root is to the absorption of medium;
(3) after having soaked, ginkgo seedling is placed in warm air drier and dries 13 minutes by the temperature of 23 degrees Celsius, oven dry can make the moisture of tiny root discharge fast, infiltration rate to liquid nutrient can be increased entering into next link, promote the absorption of nutrient;
(4) ginkgo seedling single is placed on diameter after terminating by oven dry is in the plastic bottle of 8 centimetres, then the ginkgo resin injecting 400 milliliters in plastic bottle extracts juice, and put the cool place place placement 1.5 days being neatly placed into ventilation, ginkgo seedling can be made like this before cultivating can fully to absorb nutrient of the same clan, ensure that the growth of seedling and drawing of nutrient;
(5) ready medium being poured into the degree of depth is in the cement pit of 10-20 centimetre, the medium degree of depth be 10 centimeters dark, then by above-mentioned plastic bottle ginkgo seedling take out evenly put in cement pit;
(6) putting is evenly the plastic greenhouse of 1 meter at the top antenna height of cement pit, and ensures the sealability of plastic greenhouse;
(7) then constant temperature setting is carried out to plastic greenhouse, ensure that temperature of shed remains between 40 degrees Celsius, the setting of constant temperature can ensure that the speed of seedling absorption medium is more stable, can not absorb not smooth problem, and the temperature of regulation is also carry out setting according to the characteristic of ginkgo;
(8) cultivate after 25-35 days and terminate to cultivate, ginkgo seedling can be taken out and carry out there is soil cultivation;
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (1)
1. by a method for medium culture ginkgo seedling, it is characterized in that: comprise following methods step:
(1) medium preparing:
Ginkgo leaf juicing 400-450 part
Ginkgo is with powder 800-900 part
Manganese sulphate 22-25 part
Zinc sulphate 10-15 part
Boric acid 5-8 part
Inositol 80-120 part
Sulfuric acid thiamine element 40-70 part
Nicotinic acid 1-1.5 part
Glycine 3-4 part
Sodium p-nitrophenoxide 5-10 part
Sodium onitrophenol 4-5 part
Citric acid 40-50 part
Mentioned component is put in culture dish according to this and stirs, leave standstill 24 hours under being then placed on the low temperature environment of 0-3 DEG C, allow between each composition and mutually ooze conjunction, left standstill wild Oryza species preparation and terminated;
(2) root of ginkgo seedling is cleaned up, and soak 1-2 hour with the physiological saline that concentration is 40%;
(3) after having soaked, ginkgo seedling is placed in warm air drier and dries 10-15 minute by the temperature of 20-25 degree Celsius;
(4) ginkgo seedling single is placed on diameter after terminating by oven dry is in the plastic bottle of 5-8 centimetre, and the ginkgo resin then injecting 400 milliliters in plastic bottle extracts juice, and is put the cool place place placement 1-2 days being neatly placed into ventilation;
(5) ready medium being poured into the degree of depth is in the cement pit of 10-20 centimetre, and the medium degree of depth is that 10-13 centimetre is dark, is then taken out by the ginkgo seedling in above-mentioned plastic bottle and evenly puts in cement pit;
(6) putting is evenly the plastic greenhouse of 1 meter at the top antenna height of cement pit, and ensures the sealability of plastic greenhouse;
(7) then constant temperature setting is carried out to plastic greenhouse, ensure that temperature of shed remains between 35-45 degree Celsius;
(8) cultivate after 25-35 days and terminate to cultivate, ginkgo seedling can be taken out and carry out there is soil cultivation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510924770.XA CN105557517A (en) | 2015-12-09 | 2015-12-09 | Method for culturing ginkgo seedlings by culture medium |
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| CN201510924770.XA CN105557517A (en) | 2015-12-09 | 2015-12-09 | Method for culturing ginkgo seedlings by culture medium |
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| CN105557517A true CN105557517A (en) | 2016-05-11 |
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| CN201510924770.XA Pending CN105557517A (en) | 2015-12-09 | 2015-12-09 | Method for culturing ginkgo seedlings by culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107125136A (en) * | 2017-06-08 | 2017-09-05 | 合肥市风达农业有限责任公司 | A kind of Camellia nitidissima tissue culture mating system |
| CN107466857A (en) * | 2017-09-16 | 2017-12-15 | 北京花乡花木集团有限公司 | A kind of high bar marigold tissue culture culture medium and preparation method thereof |
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2015
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| CN102113462A (en) * | 2011-03-09 | 2011-07-06 | 国家林业局泡桐研究开发中心 | Functional type gingko champignon cultivated by using medicinal plant residues and production method thereof |
| CN103070061A (en) * | 2013-01-31 | 2013-05-01 | 河南大学 | Functional bean sprout and production method thereof |
| CN103563623A (en) * | 2013-10-14 | 2014-02-12 | 合肥绿叶园林工程有限责任公司 | Ginkgo seedling planting cultivation method |
| CN104025988A (en) * | 2014-06-25 | 2014-09-10 | 金寨县冠友盆景园艺有限公司 | Soilless cultivation method of gingko plants |
| CN104642140A (en) * | 2015-03-13 | 2015-05-27 | 国家林业局竹子研究开发中心 | Rapid propagation method of oxytenanthera |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107125136A (en) * | 2017-06-08 | 2017-09-05 | 合肥市风达农业有限责任公司 | A kind of Camellia nitidissima tissue culture mating system |
| CN107466857A (en) * | 2017-09-16 | 2017-12-15 | 北京花乡花木集团有限公司 | A kind of high bar marigold tissue culture culture medium and preparation method thereof |
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Application publication date: 20160511 |