CN103070061A - Functional bean sprout and production method thereof - Google Patents
Functional bean sprout and production method thereof Download PDFInfo
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- CN103070061A CN103070061A CN2013100375489A CN201310037548A CN103070061A CN 103070061 A CN103070061 A CN 103070061A CN 2013100375489 A CN2013100375489 A CN 2013100375489A CN 201310037548 A CN201310037548 A CN 201310037548A CN 103070061 A CN103070061 A CN 103070061A
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the technical field of bean sprout production and specially discloses a functional bean sprout and a production method thereof. The method comprises the following steps: 1, adding water or alcohol to 30-65wt.% of folium cortex eucommiae, 15-30wt.% of ginseng roots and 15-40wt.% of ginkgo leaves for extracting, filtering, adding vitamin C to a filtrate, wherein the weight of the vitamin C is 0.01-0.02% of the weight of the filtrate, and adjusting the pH value of the liquid to 4.5-7.0 by using edible acid, thereby obtaining sprout-cultivating liquid, and 2, soaking bean seeds in the sprout-cultivating liquid, spraying with the sprout-cultivating liquid and/or water, and cultivating according to a conventional bean sprout cultivating method, thereby obtaining the bean sprout. Through the implementation of the production method, the folium cortex eucommiae, the ginseng roots and the ginkgo leaves are effectively utilized, a new method for compressively utilizing the traditional Chinese medicine is supplied, the prepared functional bean sprout contains the active ingredients, such as ginsenoside, which are beneficial to the health of human body, and an effective method and a research thought are supplied for developing the functional bean sprout.
Description
Technical field
The invention belongs to the bean sprouts production technical field, particularly a kind of functional form bean sprouts and production method thereof.
Background technology
The bean sprouts is the general name of moyashi, mung bean sprouts, black bean sprout and little bean sprouts, is the traditional dish of China.Ming Dynasty's Li Shizhen (1518-1593 A.D.) is pointed out in Compendium of Material Medica: only the white U.S.A in this bean sprouts is solely different, and clearing away heart-fire after the food takes care of health.It is " the beautiful matter of ice flesh ", " golden bud modest ability ", " palpus of white dragon " that ancients praise it, and the appearance of bean sprouts complies with one's wishes as one again, so people claim that again it is the dish of complying with one's wishes.Bean sprouts and bamboo shoot, bacterium are listed as vegetarian diet delicate flavour three despots.The bean sprouts also claims bud seedling dish, is that the cultivating seeds of various cereals, beans, tree class goes out edible " bud dish ", also claims " live body vegetables ".
The bean sprouts is as a kind of Vegetables, with its delicious flavour, nutritious, have the good characteristic such as health-care efficacy and very popular, its production method is easy, production technology is easy to grasp, production efficiency is high.Bean sprouts's growth cycle is short, needn't apply fertilizer in the process of growth, and suitable temperature, the sufficient moisture of assurance can rely on the nutrient growth of seed self storage, endanger without agricultural chemicals.
Especially like with the darkest consumer of being subjected to of mung bean sprouts among the bean sprouts that Japan, a large amount of mung beans of the annual import of the U.S. are used for the production of mung bean sprouts.Mung bean amino acid content in germination process increases substantially (content of glutamic acid is the highest); Lipoid material is resolved into glycerine and fatty acid by enzyme, generates at last the carbohydrate that can be absorbed by the body; Carbohydrate then is hydrolyzed to the monose that is easy to absorption of human body, has improved absorbability, and total sugar content generally reduces; Vitamin kind (Vc, VA, nicotinic acid, carotin etc.) content all increases; Mineral matter (potassium, magnesium, calcium, iron, phosphorus) content increases substantially after soaking germination.
Mung bean sprouts has very high medical value.The traditional Chinese medical science is thought, mung bean sprouts flavor cool in nature is sweet, the clear hot summer weather of energy, qualcomm meridian, all poison of solution, kidney tonifying, diuresis, detumescence, nourishing and strengthening vital, transfer the five internal organs, U.S. skin, dampness removing heat, be applicable to damp and hot stasis, eat the patients such as few body is tired, pyreticosis polydipsia, constipation, difficult urination, red eye, swell pain, the living sore of mouth and nose, can also reducing blood lipid and softening blood vessel.
The Eucommiaceae plant bark of eucommia
Eucommia ulmoidesOliv. for the Jue that the fourth season, the glacier carried over loses plant, be China second class protection plant.Bark of Eucommia Ulmoides is China's tradition rare traditional Chinese medicine, the energy filling liver kidney, and strengthening the bones and muscles, it is anti-old to make light of one's life by commiting suicide, and is used for kidney deficiency and liver, ache of the spinal column, muscles and bones is unable, has a dizzy spell etc.Folium cortex eucommiae is for " Chinese pharmacopoeia one one of version in 2010 is recorded kind, and eucommia bark, leaf and male flower contain similar active component and effect.Modern pharmacological research shows: have strengthen immunity, delay senility, the effects such as prevention of osteoporosis, activation collagen.
Ginseng derives from the Araliaceae ginseng
Panax ginsengThe dry root and rhizome of cultivation product C.A.Mey., fibrous root are called the ginseng palpus, are herbaceos perennial.It is the rare medicinal herbs that has won fame both at home and abroad.Sweet, the little hardship of ginseng flavor, slightly warm in nature has invigorating qi for relieving desertion, invigorating the spleen benefit lung, peaceful heart intelligence development, the effect of nourishing blood to promote the production of body fluid.The root of ginseng contains similar active component with rhizome, fibrous root, stem, leaf, and main component is the ginsenoside Rg
1, ginsenoside Re, ginsenoside Rb
1, panaxan etc.Modern pharmacological research shows: ginseng has: (1) regulates central nervous system; (2) promote brain to the energy matter utilization; (3) improve cardiac function; (4) hypoglycemic; (5) immunologic function of enhancing body; (6) raising is to the defensive ability/resistance ability of destructive stimulus; (7) antitumor; (8) effect such as anti-oxidant grade.
Pseudo-ginseng derives from panax araliaceae plant
Panax notoginseng(Burk.) dry root and the rhizome of F. H. Chen are herbaceos perennial.It is the rare medicinal herbs that has won fame both at home and abroad.Pseudo-ginseng, pseudo-ginseng stem, leaf contain similar active component, are mainly the ginsenoside Rg
1, Rb
1, Panax Notoginseng saponin R
1Deng, similar to the ginseng main component.
Ginkgo leaf derives from the Ginkgoaceae plant Ginkgo biloba
Ginkgo bilobaL. dried leaves.Ginkgo, another name gingko, Gong Sunshu, China's ginkgo leaf output accounts for 70% of the world, but availability is not high.Ginkgo leaf has very high medical value.Ginkgo is one of ancient seeds of China, Jurassic Period dinosaur control terrestrial time before more than 200,000,000 5 thousand year, and ginkgo is one of the most flouring plant.Life on earth is gone through the change in 100000000000 years, especially after the glacier covering of the 4th century, only has ginkgo still to keep its most original looks, is called as " living fossil " in the organic evolution history.Its leaf, fruit, seed have higher medical value, and the ginkgo leaf main active is ginkgolides and Gingko yellow ketoside.Wherein ginkgolides is potent platelet activating factor (PAF) antagonist, comprises ginkgolide A, B, C and Bilobalide etc., all has diterpene or hemiterpene structure.Ginkgo leaf has: (1) increases cerebral blood flow (CBF), the protection brain damage; (2) improve learning and memory, alleviate Auditory nerve impairment; (3) improve elderly cerebral disorder, brain insufficiency, insomnia, memory impairment; (4) coronary artery dilator, the protection ischemic myocardium reduces arrhythmia cordis and occurs; (5) remove free radical, anti-oxidant; (6) suppress the effects such as platelet aggregation, reducing blood lipid.
Ascorbic effect: (1) promotes collagen synthesis, is beneficial to wound healing, strengthens skin elasticity; (2) anti-oxidant, detoxifcation, the side effect that minimizing cigarette, wine, medicine produce and environmental pollution are to the infringement of health; (3) absorption and the utilization of promotion iron, calcium, folic acid; (4) improve the metabolism of cholesterol, the prevention cardiovascular and cerebrovascular diseases; (5) promote tooth and bone growth; (6) strengthen body to external world stress ability and the immunity of environment.
At present, the bean sprouts quality is single, and function bean sprouts with health role is less, only have at present " iodine bean sprouts " (production method of iodine bean sprouts, ZL93102789.6,1993.03.29), " splashings bud " (production method of ferro-rich bean sprouts, ZL88100007.8,1988.01.05).Enhancing along with people's health care consciousness, the particularly quickening of China's aging population process, be fit to the middle-aged and old health, have the favor that the bean sprouts that strengthens immunity, softening blood vessel, prevention cardiovascular and cerebrovascular disease, improves the health cares such as learning and memory function is subject to market.
Summary of the invention
For overcoming deficiency of the prior art, the object of the present invention is to provide a kind of functional form bean sprouts and production method thereof.
For achieving the above object, the technical scheme taked of the present invention is as follows:
The production method of a kind of functional form bean sprouts may further comprise the steps:
The first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 30～65%, ginseng fibrous root 15～30%, ginkgo leaf 15～40%, filter, add the vitamin C that accounts for its percentage by weight 0.01 ~ 0.02% in the filtrate, modulate this liquid pH value between 4.5 ~ 7.0 with food acids, must educate bud liquid;
Second step: with educating bud liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with educating bud liquid.
Preferably, the first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 30～50%, ginseng fibrous root 20～25%, ginkgo leaf 20～35%, filter, add the vitamin C that accounts for its percentage by weight 0.015% in the filtrate, modulate this liquid pH value between 4.5 ~ 7.0 with food acids, must educate bud liquid.
Preferably, described folium cortex eucommiae obtains by the following method: gather green folium cortex eucommiae annual 6～September, dries or oven dry below 60 ℃; Described ginseng fibrous root obtains by the following method: cultivate the ginseng in 5 ~ 7 years, behind White Dew solar term, after the ginseng above-ground plant parts is withered, when gathering ginseng, get the remaining fibrous root of processing, dry; Described ginkgo leaf obtains by the following method: gather ginkgo leaf, in time dry annual 9～October.
Preferably, extract 1～4 time, and 3～25 volume/weight of at every turn pressing folium cortex eucommiae, ginseng fibrous root, ginkgo leaf total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
Further, extracting mode extracts for soaking extraction, heating and refluxing extraction or sudden strain of a muscle formula, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Sudden strain of a muscle formula extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
Preferably, described beans kind is mung bean, soya bean, French beans, pea, broad bean, black soya bean, peanut, green soya bean, Chinese toon seed, buckwheat, clover, Chinese prickly ash, jequirity, sunflower seed or Semen Raphani.
Further, used folium cortex eucommiae replaces with eucommia Bark male flower or Bark of Eucommia Ulmoides; Used ginseng fibrous root employment ginseng, ginseng stem, folium panacis japonici cum caule, general ginsenoside, ginseng stem and leave general saponin, red ginseng tails, pseudo-ginseng, pseudo-ginseng stem, sanchi leaf, arasaponin or notoginseng stem and leaf total saponin replace; Used ginkgo leaf replaces with ginkgo biloba p.e or Bilobanoate (Xingling Sci. ﹠ Tech. Pharmaceutical Co., Ltd., Shanghai produces, the accurate word Z20000049 of traditional Chinese medicines).
A kind of functional form bean sprouts of aforementioned production method preparation.
The cultivation success of functional form bean sprouts takes full advantage of the bark of eucommia, ginseng, gingko resource, has opened up the new way of Chinese medicine comprehensive utilization, provides a kind of novel green functional food to people, has promoted the health-care effect of traditional bean sprouts.
Advantage of the present invention is:
1, by enforcement of the present invention, effectively utilizes folium cortex eucommiae, ginseng fibrous root, ginkgo leaf etc., for the comprehensive utilization of Chinese medicine provides new way.
2, for the single problem of bean sprouts production kind, be that raw material is made and educated bud liquid with folium cortex eucommiae, ginseng fibrous root, ginkgo leaf.Employing is educated bud liquid and is cultivated a kind of functional form bean sprouts, by to functional form bean sprouts active ingredient contrast test, find that " functional form bean sprouts " contain the active component that ginsenoside etc. is conducive to health, for the exploitation of functional bean sprouts provides effective approach and Research Thinking.
Description of drawings
Fig. 1: ginsenoside Rg
1, ginsenoside Re, ginsenoside Rb
1Mix reference substance HPLC figure;
Fig. 2: embodiment 1 functional form bean sprouts HPLC figure;
Fig. 3: embodiment 2 functional form bean sprouts HPLC figure;
Fig. 4: embodiment 3 functional form bean sprouts HPLC figure;
In Fig. 1 ~ 4, the 1--ginsenoside Rg
1The 2--ginsenoside Re; 3--ginsenoside Rb
1
Embodiment
Below in conjunction with specific embodiment technical scheme of the present invention is done further in detail introduction, but protection scope of the present invention is not limited to this:
The first step: gather green folium cortex eucommiae annual June, dries; Cultivate the ginseng in 6 years, behind White Dew solar term, after the ginseng above-ground plant parts is withered, when gathering ginseng, get the remaining fibrous root of processing, dry; Gather ginkgo leaf, in time dry annual September.
Second step: get folium cortex eucommiae 65 g, ginseng fibrous root 15 g, ginkgo leaf 20 g, extract 1 time with the 1000ml water soaking, extraction time is 6 hours, extracting temperature is 30 ℃, filter, account for the vitamin C of its percentage by weight 0.01% filtrate adding, modulates this liquid pH value 4.5 with edible citric acid, namely gets and educate bud liquid.
The 3rd step: replace the water soaking mung bean with educating bud liquid, with the mung bean of educating in the case of bud liquid spray bean sprouts.Cultivate and get final product according to conventional mung bean sprouts breeding method.As get the 100g mung bean and place 250ml to educate 26 ℃ of constant temperature of bud liquid to soak 10 hours, and after the abundant imbibition of seed, be transferred in the case of bean sprouts, cultivate the bean sprouts, temperature keeps 26 ℃.Educate bud liquid with method preparation every day according to the above ratio, keeps fresh, and per 4 hour spray bean sprouts 1 time with educating bud liquid every day, generally in the case of bean sprouts 3-4 days, during radicle plumular axis length to the 3 centimetre left and right sides, just obtains the functional form mung bean sprouts after washing with clear water.
Embodiment 2
The first step: gather green folium cortex eucommiae annual September, oven dry below 60 ℃; Cultivate the ginseng in 6 years, behind White Dew solar term, after the ginseng above-ground plant parts is withered, when gathering ginseng, get the remaining fibrous root of processing, dry; Gather ginkgo leaf, in time dry annual October.
Second step: get folium cortex eucommiae 40 g, ginseng fibrous root 30 g, ginkgo leaf 30 g, adopt flash extracter (ZL 200520031604.9) to extract 2 times, use 70v% ethanol 1500ml at every turn, each extraction time is 8 minutes, extract 25 ℃ of temperature, merge extract, filter, decompression filtrate recycling ethanol is to nothing alcohol flavor, continue to be concentrated into 500ml, account for the vitamin C of its percentage by weight 0.015% filtrate adding, modulates this liquid pH value 7.0 with edible malic acid, namely gets and educate bud liquid.
The 3rd step: replace the water soaking mung bean with educating bud liquid, the mung bean in the case of water spray bean sprouts is cultivated and be get final product according to conventional mung bean sprouts breeding method.
The first step: gather green folium cortex eucommiae annual August, oven dry below 60 ℃; Cultivate the ginseng in 6 years, behind White Dew solar term, after the ginseng above-ground plant parts is withered, when gathering ginseng, get the remaining fibrous root of processing, dry; Gather ginkgo leaf annual September, in time dry.
Second step: get folium cortex eucommiae 30 g, ginseng fibrous root 30 g, ginkgo leaf 40 g, adopt the 60v% alcohol heating reflux to extract 2 times, each with 60v% ethanol 1500ml, each extraction time is 1.5 hours, merges extract, filter, decompression filtrate recycling ethanol continues to be concentrated into 500ml to without the alcohol flavor, accounts for the vitamin C of its percentage by weight 0.02% filtrate adding, modulate this liquid pH value 6.0 with alimentary acetic acid, namely get and educate bud liquid.
The 3rd step: replace the water soaking mung bean with educating bud liquid, with the mung bean of educating in the case of bud liquid spray bean sprouts, cultivate and get final product according to conventional mung bean sprouts breeding method.
Method for measuring content of ginsenoside in the functional form mung bean sprouts
1, instrument and reagent
Agilent 1260 high performance liquid chromatographs (quaternary gradient pump, online degasser, column oven, VWD detector, ChemStation control system); Ultrasonic cleaner (KQ5200DA type, Kunshan Ultrasonic Instruments Co., Ltd.); Electronic balance (FA1004 type, upper Nereid section balance); The ginsenoside Rg
1Reference substance, ginsenoside Re's reference substance and ginsenoside Rb
1Reference substance (being National Institute for Food and Drugs Control provides, and for assay, lot number is respectively: 110703-201128,110754-201123,110704-201122); Acetonitrile is chromatographically pure (Merk company); Water is ultra-pure water, and other reagent are pure for analyzing.
Chromatographic condition and system suitability octadecylsilane chemically bonded silica are filler; Take acetonitrile as mobile phase A, take water as Mobile phase B; According to the form below carries out gradient elution (the results are shown in Table 1); The detection wavelength is 203nm; Column temperature is 10 ℃; Flow velocity is 1.0ml/min.Number of theoretical plate is by the ginsenoside Rg
1The peak calculates and is not less than 6000.
The preparation precision of reference substance solution takes by weighing the ginsenoside Rg
1Reference substance, ginsenoside Re's reference substance and ginsenoside Rb
1Reference substance adds methyl alcohol and makes the mixed solution that every 1ml contains 0.2mg, shakes up, and get final product.
The preparation of need testing solution is got this product and is ground, and accurately weighed 30g puts in the tool plug conical flask, add methyl alcohol 100ml, ultrasonic processing (power 200W, frequency 40kHz) 30 minutes, filter, filtrate decompression is concentrated into and is less than 10ml, lets cool, be transferred in the 10 ml measuring bottles, add methyl alcohol and be diluted to scale, shake up, filter, get subsequent filtrate, and get final product.
Determination method is accurate reference substance solution 10 μ l and the need testing solution 10 μ l of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
In reference substance solution and the embodiment 1-3 functional form bean sprouts content of ginsenoside see Table 2, Fig. 1-4.
Claims (8)
1. the production method of a functional form bean sprouts is characterized in that may further comprise the steps:
The first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 30～65%, ginseng fibrous root 15～30%, ginkgo leaf 15～40%, filter, add the vitamin C that accounts for its percentage by weight 0.01 ~ 0.02% in the filtrate, modulate this liquid pH value between 4.5 ~ 7.0 with food acids, must educate bud liquid;
Second step: with educating bud liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with educating bud liquid.
2. production method as claimed in claim 1, it is characterized in that the first step: in weight percentage, add water or alcohol extract by folium cortex eucommiae 30～50%, ginseng fibrous root 20～25%, ginkgo leaf 20～35%, filter, add the vitamin C that accounts for its percentage by weight 0.015% in the filtrate, modulate this liquid pH value between 4.5 ~ 7.0 with food acids, must educate bud liquid.
3. production method as claimed in claim 1, it is characterized in that: described folium cortex eucommiae obtains by the following method: gather green folium cortex eucommiae annual 6～September, dries or oven dry below 60 ℃; Described ginseng fibrous root obtains by the following method: cultivate the ginseng in 5 ~ 7 years, behind White Dew solar term, after the ginseng above-ground plant parts is withered, when gathering ginseng, get the remaining fibrous root of processing, dry; Described ginkgo leaf obtains by the following method: gather ginkgo leaf, in time dry annual 9～October.
4. production method as claimed in claim 1 is characterized in that: extracts 1～4 time, and 3～25 volume/weight of at every turn pressing folium cortex eucommiae, ginseng fibrous root, ginkgo leaf total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
5. production method as claimed in claim 4 is characterized in that: extracting mode extracts for soaking extraction, heating and refluxing extraction or sudden strain of a muscle formula, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Sudden strain of a muscle formula extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
6. production method as claimed in claim 1, it is characterized in that: described beans kind is mung bean, soya bean, French beans, pea, broad bean, black soya bean, peanut, green soya bean, Chinese toon seed, buckwheat, clover, Chinese prickly ash, jequirity, sunflower seed or Semen Raphani.
7. production method as claimed in claim 1 is characterized in that: used folium cortex eucommiae replaces with eucommia Bark male flower or Bark of Eucommia Ulmoides; Used ginseng fibrous root employment ginseng, ginseng stem, folium panacis japonici cum caule, general ginsenoside, ginseng stem and leave general saponin, red ginseng tails, pseudo-ginseng, pseudo-ginseng stem, sanchi leaf, arasaponin or notoginseng stem and leaf total saponin replace; Used ginkgo leaf replaces with ginkgo biloba p.e or Bilobanoate.
8. the functional form bean sprouts of the described production method preparation of any one of a utilization such as claim 1 ~ 7.
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