CN103053953B - Functional bean sprouts prepared from Chinese herbal medicines used as both food and medicine and method for preparing functional bean sprouts - Google Patents
Functional bean sprouts prepared from Chinese herbal medicines used as both food and medicine and method for preparing functional bean sprouts Download PDFInfo
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Abstract
The invention belongs to the technical field of bean sprout production, in particular discloses functional bean sprouts prepared from Chinese herbal medicines used as both food and medicine and a method for preparing the functional bean sprouts. The method comprises the following steps: step one, weighing 20-50% by weight of kudzuvine root, 15-50% by weight of hawthorn and 10-40% by weight of honeysuckle, adding water or ethanol to extract, filtering and adding 0.02-0.05% by weight of vitamin C to a filtrate to obtain a sprout culture solution; and step two, soaking beans with the sprout culture solution, spraying the sprout culture solution or/and water and culturing according to the conventional bean sprout culture method, thereby obtaining the bean sprouts. By adopting the method, the Chinese herbal medicines used as both the food and the medicine, such as the kudzuvine root, the hawthorn and the honeysuckle can be used effectively, so a new way is provided for the comprehensive utilization of the traditional Chinese medicine. The prepared functional bean sprouts comprise active components beneficial to human health, such as puerarin, thereby providing an effective way and a research idea for the development of the functional bean sprouts.
Description
Technical field
The invention belongs to bean sprouts production technical field, particularly a kind of medicine food that utilizes is with functional form bean sprouts and the method for producing with Chinese medicine.
Background technology
Tall scrawny person is the general name of moyashi, mung bean sprouts, black bean sprout and little bean sprouts, is the traditional dish of China.Bean sprouts and bamboo shoot, bacterium are listed as vegetarian diet delicate flavour three despots.Bean sprouts also claims bud seedling dish, is that the cultivating seeds of various cereals, beans, tree class goes out edible " bud dish ", also claims " live body vegetables ".
Tall scrawny person is as a kind of Vegetables, with its delicious flavour, nutritious, there is the good characteristics such as health-care efficacy and very popular, its production method is easy, production efficiency is high.Tall scrawny person's growth cycle is short, does not apply fertilizer, and without agricultural chemicals, endangers.
Especially most favored by customers with mung bean sprouts in tall scrawny person, Japan, a large amount of mung beans of the annual import of the U.S. are used for the production of mung bean sprouts.Mung bean amino acid content in germination process increases substantially (content of glutamic acid is the highest); Lipoid material is resolved into glycerine and aliphatic acid by enzyme, finally generates the carbohydrate that can be absorbed by the body; Carbohydrate is hydrolyzed to the monose that is easy to absorption of human body, has improved absorbability, and total sugar content generally reduces; Vitamin kind (Vc, VA, nicotinic acid, carrotene etc.) content all increases; Mineral matter (potassium, magnesium, calcium, iron, phosphorus) content increases substantially after soaking germination.
Mung bean sprouts has very high medical value.The traditional Chinese medical science is thought, mung bean sprouts taste cool in nature is sweet, the clear hot summer weather of energy, qualcomm meridian, all poison of solution, kidney tonifying, diuresis, detumescence, nourishing and strengthening vital, adjust the five internal organs, U.S. skin, dampness removing heat, be applicable to damp and hot stasis, eat the patients such as few body is tired, pyreticosis polydipsia, constipation, difficult urination, red eye, swell pain, the raw sore of mouth and nose, can also reducing blood lipid and softening blood vessel.
China's Chinese Traditional Medicine just has " integration of drinking and medicinal herbs " theory from ancient times, people have found nature and flavor and the effect of various food and medicine in the process of search of food, recognize that much food can be medicinal, many medicines are also edible, be difficult to strict differentiation between the two, in China Medical monographs < < The Yellow Emperor's Canon of Internal Medicine > > the earliest, just there is the discussion of " medicine to dispel it, following the drugs with foods "." regimen " is to have an important component part in motherland's medicine treasure-house.Utilize food health to cure the disease, among the people always by wide-spread.Integration of drinking and medicinal herbs, uses food health to cure the disease, simple, evident in efficacy, safety non-toxic.
The root of kudzu vine is legume pueraria lobata
pueraria lobata(Willd.) Ohwi. or sweet kudzu
pueraria thomsoniibanthd. dry root is used as medicine.Energy relieving muscles to expel heat, promotes the production of body fluid, and promoting eruption, rises positive antidiarrheal.For fever caused by exogenous pathogens headache, stiff nape and back, thirsty, quench one's thirst, measles without adequate eruption, hot dysentery, has loose bowels; Hypertension neck pain etc.For common medicine food is with using Chinese medicine, if kudzu-vine root powder, root of kudzu vine jelly, root of kudzu vine biscuit, root of kudzu vine Nakai beverage etc. are often edible food.Root of kudzu vine main component is osajin (Puerarin, daidzin) etc.; Modern pharmacological research shows: the root of kudzu vine has hypoglycemic, coronary artery dilator, improve myocardial metabolism, anti-arrhythmia, improve microcirculation and brain circulation, spasmolysis, the effect such as antipyretic.
Hawthorn is rosaceous plant large-fruited Chinese hawthorn
crataegus pinnatifidabge.var.major N.E.Br. or hawthorn
crataegus pinnatifidabge. dry mature fruit.Can food digesting stomach fortifying, the promoting the circulation of qi stasis of blood of faling apart.For carnivorous stagnant, gastral cavilty turgor, rushes down dysentery stomachache, and hemostasis is through closing, postpartum stasis blocking, trusted subordinate's shouting pain, hernia pain; Hyperlipidemia.For common medicine food is with using Chinese medicine.As Chang Zuowei food consumption such as haw jelly, hawthorn volume, Sugarcoated haws on a stick.Hawthorn main component is organic acid (as citric acid, malic acid etc.) and flavonoids (Hyperoside, Quercetin, rutin etc.) etc.Modern pharmacological research shows: hawthorn has hypotensive, reducing blood lipid, improves myocardial ischemia, reduces the effects such as cholesterol, anti peroxidation of lipid, is used for the treatment of cardiovascular and cerebrovascular disease and has obtained good effect.
Honeysuckle is the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb. or the flower that band is just opened.Honeysuckle is cold in nature, and taste is sweet.Return lung, the heart, stomach warp.Can be clearing heat and detoxicating, wind-heat dissipating.For the swollen furunculosis of carbuncle, larynx numbness, erysipelas, toxic-heat and blood stasis, anemopyretic cold, warm disease heating.For common medicine food is with Chinese medicine, if the distilled liquid of honeysuckle, Lonicera flower tea, honeysuckle cold tea, honeysuckle lozenge etc. are common food, for mouth parched and tongue scorched, abscess of throat etc.Honeysuckle main active is organic acid (chlorogenic acid, isochlorogenic acid etc.) and flavonoids (galuteolin, lonicerin etc.).Honeysuckle has: (1) resisting pathogenic microbes; (2) antibacterium toxin; (3) anti-inflammatory; (4) cholagogic, the effect such as protect the liver.
Ascorbic effect: (1) promotes collagen synthesis, strengthens skin elasticity; (2) anti-oxidant, removing toxic substances, reduces the infringement of environmental pollution to health; (3) promote absorption and the utilization of iron, calcium, folic acid; (4) improve the metabolism of cholesterol, prevention cardiovascular and cerebrovascular diseases; (5) promote tooth and bone growth.
At present, bean sprouts quality is single, and function bean sprouts with health role is less, only have at present " iodine bean sprouts " (production method of iodine bean sprouts, ZL93102789.6,1993.03.29), " splashings bud " (production method of ferro-rich bean sprouts, ZL88100007.8,1988.01.05).Enhancing along with people's health care consciousness, the particularly quickening of China's aging population process, be applicable to middle-aged and old health, the bean sprouts with reducing blood lipid, softening blood vessel, prevention cardiovascular and cerebrovascular disease, resisting pathogenic microbes, hepatic cholagogic, the anti-ageing health care of waiting for a long time is subject to the favor in market.
Summary of the invention
For overcoming deficiency of the prior art, the object of the present invention is to provide a kind of medicine food that utilizes with functional form bean sprouts and the method for producing with Chinese medicine.
For achieving the above object, the technical scheme that the present invention takes is as follows:
Utilize medicine food with produce a method for functional form bean sprouts with Chinese medicine, comprise the following steps:
The first step: in weight percentage, add water or alcohol extract by the root of kudzu vine 20～50%, hawthorn 15～50%, honeysuckle 10～40%, filter, add the vitamin C that accounts for its percentage by weight 0.02 ~ 0.05% in filtrate, must educate bud liquid;
Second step: with educating bud liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with educating bud liquid.
Preferably, the first step: in weight percentage, add water or alcohol extract by the root of kudzu vine 30～40%, hawthorn 30～40%, honeysuckle 20～30%, filter, add the vitamin C that accounts for its percentage by weight 0.03% in filtrate, must educate bud liquid.
Preferably, extract 1 ~ 4 time, and 3 ~ 25 volume/weight of at every turn pressing the root of kudzu vine, hawthorn, honeysuckle total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
Further, extracting mode is for soaking extraction, refluxing extraction or flash extraction, and extracting mode, for soaking extraction, refluxing extraction or flash extraction, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Flash extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
Preferably, described beans kind is mung bean, soya bean, French beans, pea, sword bean, rde bean, broad bean, black soya bean, peanut, green soya bean, Chinese toon seed, buckwheat, clover, Chinese prickly ash, jequirity, sunflower seeds or Semen Raphani.
The root of kudzu vine used, hawthorn, one or several in honeysuckle are used fennel seeds, field thistle, Chinese yam, hawthorn, purslane, dark plum, pawpaw, bitter orange flower, radix polygonati officinalis, Radix Glycyrrhizae, the root of Dahurain angelica, gingko, Flos Semen Lablab Album, arillus longan, cassia seed, lily, emblic, Buddha's hand, almond is (sweet, bitter), sea-buckthorn, Gorgon fruit, Fructus Hordei Germinatus, jujube (date, wild jujube, dateplum persimmon), Momordica grosvenori, brush-cherry seed, honeysuckle, Chinese olive, cordate houttuynia, ginger (ginger, rhizoma zingiberis), hoveniae semoveniae semen, the fruit of Chinese wolfberry, cape jasmine, fructus amomi, the sterculia seed, Poria cocos, citron, elscholtiza, peach kernel, mulberry leaf, mulberry fruit, orange, balloonflower root, fructus alpiniae oxyphyllae, lotus leaf, radish seed, lotus seeds, galangal, lophatherum gracile, chrysanthemum, witloof, SEMEN SINAPIS ALBAE, sealwort, purple perilla, the root of kudzu vine, Semen sesami nigrum, the sophora bud, sophora flower, dandelion, honey, Chinese torreyanut, spina date seed, fresh Rhizoma Imperatae, fresh Rhizoma Phragmitis, orange peel, peppermint, coix seed, Longstamen Onion Bulb, raspberry, the medicine foods such as wrinkled giant hyssop are with one or several replacements with in Chinese medicine.
Functional form bean sprouts prepared by a kind of aforementioned production method.
The cultivation success of functional form bean sprouts, take full advantage of the medicine foods such as the root of kudzu vine, hawthorn, honeysuckle with using Chinese material medicine resource, open up medicine food with the new way with Chinese medicine comprehensive utilization, to people, provide a kind of novel green functional food, promoted the health-care effect of traditional bean sprouts.
Advantage of the present invention is:
1, by enforcement of the present invention, effectively utilize the medicine foods such as the root of kudzu vine, hawthorn, honeysuckle with Chinese medicine, the integration of drinking and medicinal herbs theory and practice of China's traditional chinese medicine is innovated and explained, for medicine food is with providing new way with the comprehensive utilization of Chinese medicine.
2,, for the single problem of bean sprouts production kind, by the root of kudzu vine, hawthorn, honeysuckle, be that raw material is made and educated bud liquid.Employing is educated bud liquid and is cultivated a kind of functional form bean sprouts, by to functional form bean sprouts active ingredient contrast test, find that " functional form bean sprouts " contain the active component that Puerarin etc. is conducive to health, for the exploitation of functional bean sprouts provides effective approach and Research Thinking.
Accompanying drawing explanation
Fig. 1: Puerarin reference substance HPLC figure;
Fig. 2: embodiment 1 functional form bean sprouts HPLC figure;
Fig. 3: embodiment 2 functional form bean sprouts HPLC figure;
Fig. 4: embodiment 3 functional form bean sprouts HPLC figure;
The specific embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is done further and introduced in detail, but protection scope of the present invention is not limited to this:
Embodiment 1
The first step: get the root of kudzu vine 40 g, hawthorn 40 g, honeysuckle 20 g, extract 1 time with 1000ml water soaking, extraction time is 10 hours, and extracting temperature is 25 ℃, filters, and accounts for the vitamin C of its percentage by weight 0.03% filtrate adding, and must educate bud liquid.
Second step: replace water soaking mung bean with educating bud liquid, with the mung bean of educating in the case of bud liquid spray bean sprouts.According to conventional mung bean sprouts breeding method, cultivate and get final product.As get 100g mung bean and be placed in 250ml and educate 26 ℃ of constant temperature of bud liquid and soak 10 hours, after the abundant imbibition of seed, be transferred in the case of bean sprouts, cultivate bean sprouts, temperature keeps 28 ℃.Educate bud liquid with method preparation every day according to the above ratio, keeps fresh, and every 4 hour spray bean sprouts 1 time with educating bud liquid every day, and generally 3-4 days in the case of bean sprouts, when radicle plumular axis grows to 3 centimetres of left and right, just obtains functional form mung bean sprouts after rinsing with clear water.
Embodiment 2
The first step: get the root of kudzu vine 45 g, hawthorn 40 g, honeysuckle 15 g, with 2000ml 40v% alcohol heating reflux, extract 1 time, extraction time is 2 hours, extracting liquid filtering, decompression filtrate recycling ethanol is extremely without alcohol taste, filter, account for the vitamin C of its percentage by weight 0.03% filtrate adding, must educate bud liquid.
Second step: replace water soaking mung bean with educating bud liquid, the mung bean in the case of water spray bean sprouts, cultivates and get final product according to conventional mung bean sprouts breeding method.
Embodiment 3
The first step: get the root of kudzu vine 25 g, hawthorn 45 g, honeysuckle 30 g, adopt flash extracter (ZL 200520031604.9) to extract 3 times, each 1000ml water of using, extract 5 minutes, extracting temperature is 30 ℃, merges extract, filters, account for the vitamin C of its percentage by weight 0.03% filtrate adding, must educate bud liquid.
Second step: replace water soaking mung bean with educating bud liquid, with the mung bean of educating in the case of bud liquid spray bean sprouts, cultivate and get final product according to conventional mung bean sprouts breeding method.
puerarin content assay method in functional form mung bean sprouts
1, instrument and reagent
Agilent 1260 high performance liquid chromatographs (quaternary gradient pump, online degasser, column oven, VWD detector, ChemStation control system); Ultrasonic cleaner (KQ5200DA type, Kunshan Ultrasonic Instruments Co., Ltd.); Electronic balance (FA1004 type, upper Nereid section balance); Puerarin reference substance (National Institute for Food and Drugs Control provides, for assay, and lot number:: 110752-200912); Methyl alcohol is chromatographically pure (Merk company); Water is ultra-pure water, and other reagent are pure for analyzing.
Chromatographic condition and system suitability octadecylsilane chemically bonded silica are filler; Mobile phase: methanol-water (volume ratio, 33: 67); Column temperature is 25 ℃; Detect wavelength: 250nm; Flow velocity: 1mL/min; Number of theoretical plate calculates and is not less than 4000 by Puerarin.
It is appropriate that Puerarin reference substance is got in the preparation of reference substance solution, accurately weighed, adds 30v% ethanol and make every 1ml containing the solution of 51 μ g, obtains.
The preparation of need testing solution is got this product fresh sample and is ground, and accurately weighed approximately 10 g, put in tool plug conical flask, add 30 v % ethanol 50ml, weighed weight, adds hot reflux 30 minutes, let cool, filter, filtrate decompression is concentrated into and is less than 10ml, let cool, be transferred in 10 ml measuring bottles, add 30 v % ethanol and be diluted to scale, shake up, filter, get subsequent filtrate, obtain.
Determination method is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and injection liquid chromatography, measures, and obtains.
In reference substance solution and embodiment 1-3 functional form bean sprouts the content of Puerarin in Table 1, Fig. 1-4.
Claims (6)
1. utilize medicine food with produce a method for functional form bean sprouts with Chinese medicine, it is characterized in that comprising the following steps:
The first step: in weight percentage, add water or alcohol extract by the root of kudzu vine 20～50%, hawthorn 15～50%, honeysuckle 10～40%, filter, add the vitamin C that accounts for its percentage by weight 0.02 ~ 0.05% in filtrate, must educate bud liquid;
Second step: with educating bud liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with educating bud liquid.
2. production method as claimed in claim 1, it is characterized in that the first step: in weight percentage, by the root of kudzu vine 30～40%, hawthorn 30～40%, honeysuckle 20～30%, add water or alcohol extract, filter, in filtrate, add the vitamin C that accounts for its percentage by weight 0.03%, must educate bud liquid.
3. production method as claimed in claim 1, is characterized in that: extracts 1 ~ 4 time, and 3 ~ 25 volume/weight of at every turn pressing the root of kudzu vine, hawthorn, honeysuckle total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
4. production method as claimed in claim 4, is characterized in that: extracting mode, for soaking extraction, refluxing extraction or flash extraction, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Flash extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
5. production method as claimed in claim 1, is characterized in that: described beans kind is mung bean, soya bean, French beans, pea, broad bean, black soya bean, sword bean, rde bean, peanut, green soya bean or jequirity.
6. the functional form bean sprouts that prepared by the utilization production method as described in any one of claim 1 ~ 5.
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