CN103053953A - Functional bean sprouts prepared from Chinese herbal medicines used as both food and medicine and method for preparing functional bean sprouts - Google Patents
Functional bean sprouts prepared from Chinese herbal medicines used as both food and medicine and method for preparing functional bean sprouts Download PDFInfo
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Abstract
The invention belongs to the technical field of bean sprout production, in particular discloses functional bean sprouts prepared from Chinese herbal medicines used as both food and medicine and a method for preparing the functional bean sprouts. The method comprises the following steps: step one, weighing 20-50% by weight of kudzuvine root, 15-50% by weight of hawthorn and 10-40% by weight of honeysuckle, adding water or ethanol to extract, filtering and adding 0.02-0.05% by weight of vitamin C to a filtrate to obtain a sprout culture solution; and step two, soaking beans with the sprout culture solution, spraying the sprout culture solution or/and water and culturing according to the conventional bean sprout culture method, thereby obtaining the bean sprouts. By adopting the method, the Chinese herbal medicines used as both the food and the medicine, such as the kudzuvine root, the hawthorn and the honeysuckle can be used effectively, so a new way is provided for the comprehensive utilization of the traditional Chinese medicine. The prepared functional bean sprouts comprise active components beneficial to human health, such as puerarin, thereby providing an effective way and a research idea for the development of the functional bean sprouts.
Description
Technical field
The invention belongs to the bean sprouts production technical field, particularly a kind of medicine food that utilizes is with functional form bean sprouts and the method for producing with Chinese medicine.
Background technology
The tall scrawny person is the general name of moyashi, mung bean sprouts, black bean sprout and little bean sprouts, is the traditional dish of China.Bean sprouts and bamboo shoot, bacterium are listed as vegetarian diet delicate flavour three despots.The bean sprouts also claims bud seedling dish, is that the cultivating seeds of various cereals, beans, tree class goes out edible " bud dish ", also claims " live body vegetables ".
The tall scrawny person is as a kind of Vegetables, with its delicious flavour, nutritious, have the good characteristics such as health-care efficacy and very popular, its production method is easy, production efficiency is high.Tall scrawny person's growth cycle is short, does not apply fertilizer, and endangers without agricultural chemicals.
Especially most favored by customers with mung bean sprouts among the tall scrawny person, Japan, a large amount of mung beans of the annual import of the U.S. are used for the production of mung bean sprouts.Mung bean amino acid content in germination process increases substantially (content of glutamic acid is the highest); Lipoid material is resolved into glycerine and aliphatic acid by enzyme, generates at last the carbohydrate that can be absorbed by the body; Carbohydrate then is hydrolyzed to the monose that is easy to absorption of human body, has improved absorbability, and total sugar content generally reduces; Vitamin kind (Vc, VA, nicotinic acid, carrotene etc.) content all increases; Mineral matter (potassium, magnesium, calcium, iron, phosphorus) content increases substantially after soaking germination.
Mung bean sprouts has very high medical value.The traditional Chinese medical science is thought, mung bean sprouts flavor cool in nature is sweet, the clear hot summer weather of energy, qualcomm meridian, all poison of solution, kidney tonifying, diuresis, detumescence, nourishing and strengthening vital, transfer the five internal organs, U.S. skin, dampness removing heat, be applicable to damp and hot stasis, eat the patients such as few body is tired, pyreticosis polydipsia, constipation, difficult urination, red eye, swell pain, the living sore of mouth and nose, can also reducing blood lipid and softening blood vessel.
China's Chinese Traditional Medicine just has " integration of drinking and medicinal herbs " theory from ancient times, people have found nature and flavor and the effect of various foods and medicine in the process of search of food, recognize that many foods can be medicinal, many medicines are also edible, be difficult to strict the differentiation between the two, the discussion of " medicine is to dispel it, following the drugs with foods " is just arranged in China's Medical monographs Huangdi's Internal Classics the earliest." regimen " is in motherland's medicine treasure-house an important component part to be arranged.Utilize the food health to cure the disease, among the people always by wide-spread.Integration of drinking and medicinal herbs uses the food health to cure the disease, and is simple, evident in efficacy, safety non-toxic.
The root of kudzu vine is legume pueraria lobata
Pueraria lobata(Willd.) Ohwi. or sweet kudzu
Pueraria thomsoniiBanthd. dry root is used as medicine.The energy relieving muscles to expel heat promotes the production of body fluid, and promoting eruption rises positive antidiarrheal.Be used for fever caused by exogenous pathogens headache, stiff nape and back, thirsty, quench one's thirst, measles without adequate eruption, hot dysentery is had loose bowels; Hypertension neck pain etc.Using together Chinese medicine for the common medicine food, is often edible food such as kudzu-vine root powder, root of kudzu vine jelly, root of kudzu vine biscuit, root of kudzu vine Nakai beverage etc.Root of kudzu vine main component is osajin (Puerarin, daidzin) etc.; Modern pharmacological research shows: the root of kudzu vine has hypoglycemic, coronary artery dilator, improve myocardial metabolism, anti-arrhythmia, improve microcirculation and brain circulation, spasmolysis, the effect such as analgesic.
Hawthorn is the rosaceous plant large-fruited Chinese hawthorn
Crataegus pinnatifidaBge.var.major N.E.Br. or hawthorn
Crataegus pinnatifidaBge. dry mature fruit.Can food digesting stomach fortifying, the promoting the circulation of qi stasis of blood of faling apart.Be used for carnivorous stagnantly, the gastral cavilty turgor rushes down dysentery stomachache, and hemostasis is through closing, postpartum stasis blocking, trusted subordinate's shouting pain, hernia pain; Hyperlipidemia.For the common medicine food is used together Chinese medicine.Such as Chang Zuowei food consumption such as haw jelly, hawthorn volume, Sugarcoated haws on a stick.The hawthorn main component is organic acid (such as citric acid, malic acid etc.) and flavonoids (Hyperoside, Quercetin, rutin etc.) etc.Modern pharmacological research shows: hawthorn has hypotensive, reducing blood lipid, improves myocardial ischemia, reduces the effects such as cholesterol, anti peroxidation of lipid, is used for the treatment of cardiovascular and cerebrovascular disease and has obtained good effect.
Honeysuckle is the dry flower of caprifoliaceae plant honeysuckle Lonicera japonica Thunb. or the flower that band is just opened.Honeysuckle is cold in nature, and it is sweet to distinguish the flavor of.Return lung, the heart, stomach warp.Can be clearing heat and detoxicating, wind-heat dissipating.Be used for the swollen furunculosis of carbuncle, larynx numbness, erysipelas, toxic-heat and blood stasis, anemopyretic cold, warm disease heating.Using Chinese medicine together for the common medicine food, is common food such as the distilled liquid of honeysuckle, Lonicera flower tea, honeysuckle cold tea, honeysuckle lozenge etc., is used for mouth parched and tongue scorched, abscess of throat etc.The honeysuckle main active is organic acid (chlorogenic acid, isochlorogenic acid etc.) and flavonoids (galuteolin, lonicerin etc.).Honeysuckle has: (1) resisting pathogenic microbes; (2) antibacterium toxin; (3) anti-inflammatory; (4) cholagogic, the effect such as protect the liver.
Ascorbic effect: (1) promotes collagen synthesis, strengthens skin elasticity; (2) anti-oxidant, detoxifcation, environmental contamination reduction is to the infringement of health; (3) absorption and the utilization of promotion iron, calcium, folic acid; (4) improve the metabolism of cholesterol, the prevention cardiovascular and cerebrovascular diseases; (5) promote tooth and bone growth.
At present, the bean sprouts quality is single, and function bean sprouts with health role is less, only have at present " iodine bean sprouts " (production method of iodine bean sprouts, ZL93102789.6,1993.03.29), " splashings bud " (production method of ferro-rich bean sprouts, ZL88100007.8,1988.01.05).Enhancing along with people's health care consciousness, the particularly quickening of China's aging population process, be fit to the middle-aged and old health, the bean sprouts with reducing blood lipid, softening blood vessel, prevention cardiovascular and cerebrovascular disease, resisting pathogenic microbes, hepatic cholagogic, the anti-ageing health care of waiting for a long time is subject to the favor in market.
Summary of the invention
For overcoming deficiency of the prior art, the object of the present invention is to provide a kind of medicine food that utilizes with functional form bean sprouts and the method for producing with Chinese medicine.
For achieving the above object, the technical scheme taked of the present invention is as follows:
A kind of medicine food that utilizes may further comprise the steps with the method for producing the functional form bean sprouts with Chinese medicine:
The first step: in weight percentage, add water or alcohol extract by the root of kudzu vine 20～50%, hawthorn 15～50%, honeysuckle 10～40%, filter, add the vitamin C that accounts for its percentage by weight 0.02 ~ 0.05% in the filtrate, must educate bud liquid;
Second step: with educating bud liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with educating bud liquid.
Preferably, the first step: in weight percentage, add water or alcohol extract by the root of kudzu vine 30～40%, hawthorn 30～40%, honeysuckle 20～30%, filter, add the vitamin C that accounts for its percentage by weight 0.03% in the filtrate, must educate bud liquid.
Preferably, extract 1 ~ 4 time, and 3 ~ 25 volume/weight of at every turn pressing the root of kudzu vine, hawthorn, honeysuckle total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
Further, extracting mode extracts for soaking extraction, refluxing extraction or sudden strain of a muscle formula, and extracting mode extracts for soaking extraction, refluxing extraction or sudden strain of a muscle formula, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Sudden strain of a muscle formula extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
Preferably, described beans kind is mung bean, soya bean, French beans, pea, sword bean, rde bean, broad bean, black soya bean, peanut, green soya bean, Chinese toon seed, buckwheat, clover, Chinese prickly ash, jequirity, sunflower seeds or Semen Raphani.
The used root of kudzu vine, hawthorn, in the honeysuckle one or several are used fennel seeds, field thistle, Chinese yam, hawthorn, purslane, dark plum, pawpaw, bitter orange flower, radix polygonati officinalis, Radix Glycyrrhizae, the root of Dahurain angelica, gingko, Flos Semen Lablab Album, arillus longan, cassia seed, lily, emblic, Buddha's hand, almond is (sweet, bitter), sea-buckthorn, Gorgon fruit, Fructus Hordei Germinatus, jujube (date, wild jujube, dateplum persimmon), Momordica grosvenori, brush-cherry seed, honeysuckle, Chinese olive, cordate houttuynia, ginger (ginger, rhizoma zingiberis), hoveniae semoveniae semen, the fruit of Chinese wolfberry, cape jasmine, fructus amomi, the sterculia seed, Poria cocos, citron, elscholtiza, peach kernel, mulberry leaf, mulberry fruit, orange, balloonflower root, fructus alpiniae oxyphyllae, lotus leaf, radish seed, lotus seeds, galangal, lophatherum gracile, chrysanthemum, witloof, SEMEN SINAPIS ALBAE, sealwort, purple perilla, the root of kudzu vine, Semen sesami nigrum, the sophora bud, sophora flower, dandelion, honey, Chinese torreyanut, spina date seed, fresh Rhizoma Imperatae, fresh Rhizoma Phragmitis, orange peel, peppermint, coix seed, Longstamen Onion Bulb, raspberry, the medicines such as wrinkled giant hyssop food is with one or several replacements of using in the Chinese medicine.
A kind of functional form bean sprouts of aforementioned production method preparation.
The cultivation success of functional form bean sprouts, take full advantage of the medicine foods such as the root of kudzu vine, hawthorn, honeysuckle with using Chinese material medicine resource, open up the medicine food with the new way with the Chinese medicine comprehensive utilization, provide a kind of novel green functional food to people, promoted the health-care effect of traditional bean sprouts.
Advantage of the present invention is:
1, by enforcement of the present invention, effectively utilize the medicine foods such as the root of kudzu vine, hawthorn, honeysuckle with using Chinese medicine, the integration of drinking and medicinal herbs theory and practice of China's traditional chinese medicine is innovated and explained, for the medicine food provides new way with the comprehensive utilization with Chinese medicine.
2, for the single problem of bean sprouts production kind, be that raw material is made and educated bud liquid with the root of kudzu vine, hawthorn, honeysuckle.Employing is educated bud liquid and is cultivated a kind of functional form bean sprouts, by to functional form bean sprouts active ingredient contrast test, find that " functional form bean sprouts " contain the active component that Puerarin etc. is conducive to health, for the exploitation of functional bean sprouts provides effective approach and Research Thinking.
Description of drawings
Fig. 1: Puerarin reference substance HPLC figure;
Fig. 2: embodiment 1 functional form bean sprouts HPLC figure;
Fig. 3: embodiment 2 functional form bean sprouts HPLC figure;
Fig. 4: embodiment 3 functional form bean sprouts HPLC figure;
The specific embodiment
Below in conjunction with specific embodiment technical scheme of the present invention is done further in detail introduction, but protection scope of the present invention is not limited to this:
Embodiment 1
The first step: get the root of kudzu vine 40 g, hawthorn 40 g, honeysuckle 20 g, extract 1 time with the 1000ml water soaking, extraction time is 10 hours, and extracting temperature is 25 ℃, filters, and account for the vitamin C of its percentage by weight 0.03% filtrate adding, namely gets and educate bud liquid.
Second step: replace the water soaking mung bean with educating bud liquid, with the mung bean of educating in the case of bud liquid spray bean sprouts.Cultivate and get final product according to conventional mung bean sprouts breeding method.As get the 100g mung bean and place 250ml to educate 26 ℃ of constant temperature of bud liquid to soak 10 hours, and after the abundant imbibition of seed, be transferred in the case of bean sprouts, cultivate the bean sprouts, temperature keeps 28 ℃.Educate bud liquid with method preparation every day according to the above ratio, keeps fresh, and per 4 hour spray bean sprouts 1 time with educating bud liquid every day, generally in the case of bean sprouts 3-4 days, during radicle plumular axis length to the 3 centimetre left and right sides, just obtains the functional form mung bean sprouts after washing with clear water.
Embodiment 2
The first step: get the root of kudzu vine 45 g, hawthorn 40 g, honeysuckle 15 g, extract 1 time with 2000ml 40v% alcohol heating reflux, extraction time is 2 hours, extracting liquid filtering, decompression filtrate recycling ethanol is to distinguishing the flavor of without alcohol, filter, account for the vitamin C of its percentage by weight 0.03% filtrate adding, namely gets and educate bud liquid.
Second step: replace the water soaking mung bean with educating bud liquid, the mung bean in the case of water spray bean sprouts is cultivated and be get final product according to conventional mung bean sprouts breeding method.
Embodiment 3
The first step: get the root of kudzu vine 25 g, hawthorn 45 g, honeysuckle 30 g, adopt flash extracter (ZL 200520031604.9) to extract 3 times, each 1000ml water of using, extracted 5 minutes, extracting temperature is 30 ℃, merges extract, filters, account for the vitamin C of its percentage by weight 0.03% filtrate adding, namely gets and educate bud liquid.
Second step: replace the water soaking mung bean with educating bud liquid, with the mung bean of educating in the case of bud liquid spray bean sprouts, cultivate and get final product according to conventional mung bean sprouts breeding method.
Puerarin content assay method in the functional form mung bean sprouts
1, instrument and reagent
Agilent 1260 high performance liquid chromatographs (quaternary gradient pump, online degasser, column oven, VWD detector, ChemStation control system); Ultrasonic cleaner (KQ5200DA type, Kunshan Ultrasonic Instruments Co., Ltd.); Electronic balance (FA1004 type, upper Nereid section balance); The Puerarin reference substance (National Institute for Food and Drugs Control provides, for assay, and lot number:: 110752-200912); Methyl alcohol is chromatographically pure (Merk company); Water is ultra-pure water, and other reagent are pure for analyzing.
Chromatographic condition and system suitability octadecylsilane chemically bonded silica are filler; Mobile phase: methanol-water (volume ratio, 33: 67); Column temperature is 25 ℃; Detect wavelength: 250nm; Flow velocity: 1mL/min; Number of theoretical plate calculates by Puerarin and is not less than 4000.
It is an amount of that the Puerarin reference substance is got in the preparation of reference substance solution, accurately weighed, adds 30v% ethanol and make the solution that every 1ml contains 51 μ g, and get final product.
The preparation of need testing solution is got this product fresh sample and is ground, and accurately weighed about 10 g put in the tool plug conical flask, add 30 v % ethanol 50ml, weighed weight added hot reflux 30 minutes, let cool, filter, filtrate decompression is concentrated into and is less than 10ml, let cool, be transferred in the 10 ml measuring bottles, add 30 v % ethanol and be diluted to scale, shake up, filter, get subsequent filtrate, and get final product.
Determination method is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, and the injection liquid chromatography is measured, and be get final product.
In reference substance solution and the embodiment 1-3 functional form bean sprouts content of Puerarin see Table 1, Fig. 1-4.
Claims (7)
1. one kind is utilized the medicine food with the method for producing the functional form bean sprouts with Chinese medicine, it is characterized in that may further comprise the steps:
The first step: in weight percentage, add water or alcohol extract by the root of kudzu vine 20～50%, hawthorn 15～50%, honeysuckle 10～40%, filter, add the vitamin C that accounts for its percentage by weight 0.02 ~ 0.05% in the filtrate, must educate bud liquid;
Second step: with educating bud liquid soaking beans kind,, cultivate and get final product according to conventional bean sprout cultivation method or/and water sprays with educating bud liquid.
2. production method as claimed in claim 1, it is characterized in that the first step: in weight percentage, add water or alcohol extract by the root of kudzu vine 30～40%, hawthorn 30～40%, honeysuckle 20～30%, filter, add the vitamin C that accounts for its percentage by weight 0.03% in the filtrate, must educate bud liquid.
3. production method as claimed in claim 1 is characterized in that: extracts 1 ~ 4 time, and 3 ~ 25 volume/weight of at every turn pressing the root of kudzu vine, hawthorn, honeysuckle total amount extraordinarily water or ethanol, wherein volume/weight is in ml/g.
4. production method as claimed in claim 4 is characterized in that: extracting mode extracts for soaking extraction, refluxing extraction or sudden strain of a muscle formula, soaks extraction conditions: 4～80 ℃ of temperature, time 0.5～12h; Refluxing extraction condition: time 0.2～3h; Sudden strain of a muscle formula extraction conditions: 15～60 ℃ of temperature, time 0.1～30min.
5. production method as claimed in claim 1, it is characterized in that: described beans kind is mung bean, soya bean, French beans, pea, broad bean, black soya bean, sword bean, rde bean, peanut, green soya bean, Chinese toon seed, buckwheat, clover, Chinese prickly ash, jequirity, sunflower seeds or Semen Raphani.
6. production method as claimed in claim 1 is characterized in that: the used root of kudzu vine, hawthorn, in the honeysuckle one or several are used fennel seeds, field thistle, Chinese yam, hawthorn, purslane, dark plum, pawpaw, bitter orange flower, radix polygonati officinalis, Radix Glycyrrhizae, the root of Dahurain angelica, gingko, Flos Semen Lablab Album, arillus longan, cassia seed, lily, emblic, Buddha's hand, almond, sea-buckthorn, Gorgon fruit, Fructus Hordei Germinatus, jujube, Momordica grosvenori, brush-cherry seed, honeysuckle, Chinese olive, cordate houttuynia, ginger, hoveniae semoveniae semen, the fruit of Chinese wolfberry, cape jasmine, fructus amomi, the sterculia seed, Poria cocos, citron, elscholtiza, peach kernel, mulberry leaf, mulberry fruit, orange, balloonflower root, fructus alpiniae oxyphyllae, lotus leaf, radish seed, lotus seeds, galangal, lophatherum gracile, chrysanthemum, witloof, SEMEN SINAPIS ALBAE, sealwort, purple perilla, the root of kudzu vine, Semen sesami nigrum, the sophora bud, sophora flower, dandelion, honey, Chinese torreyanut, spina date seed, fresh Rhizoma Imperatae, fresh Rhizoma Phragmitis, orange peel, peppermint, coix seed, Longstamen Onion Bulb, raspberry, one or several replacements in the wrinkled giant hyssop.
7. the functional form bean sprouts of the described production method preparation of any one of a utilization such as claim 1 ~ 6.
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