CN105534827B - A kind of toothpaste containing lysozyme - Google Patents
A kind of toothpaste containing lysozyme Download PDFInfo
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- CN105534827B CN105534827B CN201610085933.4A CN201610085933A CN105534827B CN 105534827 B CN105534827 B CN 105534827B CN 201610085933 A CN201610085933 A CN 201610085933A CN 105534827 B CN105534827 B CN 105534827B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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Abstract
The present invention relates to a kind of toothpaste containing lysozyme, and the wherein weight ratio of active component lysozyme and prostrate chrysanthemum Notogineng Extract is (1:10)~(10:1).Compared with common Chinese herbal toothpaste, lysozyme toothpaste preparation method of the invention is simple, and compatibility is reasonable, and for two kinds of active components while respective effect is played, and can produces synergistic function, significantly prevents and treats oral cavity gingivitis and bacterial plaque.Lysozyme and Gynura procumbens (Lour.) Merr source are natural, safe and non-toxic, have no side effect；Because, without western medicine composition, therefore toothpaste has no drug resistance in toothpaste.
Description
Technical field
The present invention relates to oral hygiene field, and in particular to a kind of toothpaste.
Background technology
It medically will be around and be covered in periodontal soft tissue being referred to as gum, the acute and chronic for betiding gingiva tissue is scorching
Disease is referred to as gingivitis.Bleeding gums are shown as, red and swollen, distending pain, continues development and invades sclerous tissues, produce periodontitis, including gum
Performance of the inflammation and general disease of tissue in gum.Bacterial plaque is the adhered to one another of matrix parcel or is adhered between facing, tooth
Or the bacillary colony of the soft and non-mineralising on dummy surface, for a kind of Bacterial biofilms fallen can not be washed away or gargled by water.
When bacterial plaque amount is less, it is difficult what is observed to be visually, can generally be shown well with plaque indicator agents.
Toothpaste is a kind of preparation that auxiliary is brushed teeth, and can strengthen the frictional force brushed teeth, and help removes food scraps, soft dirt and tooth
Bacterial plaque, help to eliminate or mitigate oral peculiar smell.By mechanical methods, bacterial plaque or preventing and treating gingivitis are thoroughly removed to big
It is difficult to accomplish for majority.Therefore, researcher proposes to add certain antiseptic in toothpaste.At present, it is used to prevent on the market
The antiseptic controlled in the feature toothpaste of gingivitis and bacterial plaque is mainly Western medicine, long-term use of easily to produce drug resistance.Biology
Enzyme toothpaste is a kind of toothpaste containing the composition such as bioenzyme activity composition and enzyme stabilizers, activator, and it can be further
Bactericidal, promote oral cavity further to clean, so as to take care of one's teeth, strengthen the defense system in oral cavity itself.
Lysozyme (lysozyme) is also known as muramidase (muramidase) or N-acetylmuramide lycanohydrlase (N-
Acetylmuramide glycanohydrlase), it is a kind of alkaline enzyme that can be hydrolyzed and polysaccharide is sticked in pathogenic bacteria.Mainly by broken
The β between -acetylmuramic acid and NAG-Isosorbide-5-Nitrae glycosidic bond in bad cell wall, make cell membrane insoluble glutinous more
Sugar decomposition causes cell wall rupture content to escape and make bacterolysis into soluble glycopeptide.Lysozyme can also with it is negatively charged
Virus protein directly in conjunction with, with DNA, RNA, apoprotein formed double salt, make virally inactivated.Therefore, the enzyme have antibacterial,
Anti-inflammatory, antiviral etc. act on.Lysozyme (also referred to as lyase) is the enzyme that a molecular weight is 14.4kDa, and it can pass through catalysis
In peptide glycan between -acetylmuramic acid and 2-Acetamido-2-deoxy-D-glucose residue and in chitodextrin between N-acetyl-glucosamine residue 1,
The hydrolysis of 4- β chains, and destroy the cell membrane of bacterium.In human body, lysozyme in cell juice, as saliva, tears and its
It is widely present in his some body fluid；Exist in the cytoplasmic granule body in mitochondria.In addition, also have in egg white substantial amounts of molten
Bacterium enzyme.Lysozyme is widely used because of the alkali hydrolysising protease of the cell membrane for acting on purpose microorganism that can be single-minded
In industries such as food, feed, medicine.
By the simple alkaline globulin of 18 kinds of 129 Amino acid profiles, molecular weight is lysozyme (lysozyme)
14.4KDa.Chemical property is highly stable.When pH value is acute variation in the range of 1.2~11.3, its structure is almost unchanged.
In sour environment, lysozyme is very strong to the stability of heat；When pH value is 4~7,100 DEG C of processing 1min remain to keep protoenzyme to live
Property；100 DEG C of heating 45min are resistant to when pH value is 3；In dry conditions, lysozyme can be deposited in room temperature for a long time, its
Sterling is white or slightly yellow.The crystalline solid or amorphous powder of yellow, it is odorless, it is sweet.It is soluble in water, by alkali destructible, no
It is dissolved in acetone and ether.As medical supplies, lysozyme have the advantages that good biocompatibility, to organize it is non-stimulated, non-toxic.
Lysozyme is widely present in the poultry, tear of the egg white of birds and mammal, saliva, blood plasma, urine, milk, white
Cell and other body fluid and tissue (such as liver, kidney) are intracellular；From the plant such as barley, fig, pawpaw and cabbage, radish
Lysozyme can be isolated, wherein using content in egg white as highest (containing about 0.3%).And the bacteriolyze enzyme activity in human milk, tears, saliva
Property be far above the activity of the lysozyme in (about 3 times) or other sources in egg white.Lysozyme in egg is animal lysozyme
Typical Representative, be research object important in lysozyme group, and it is currently understood that one of most clear lysozyme.Moved in lactation
In the milk of thing, the lysozyme content in human milk is 3000 times of cow's milk.It is by sources different, it is molten lysozyme can be divided into egg white
Bacterium enzyme, animal lysozyme, plant lysozyme, lysozyme caused by microorganism and lysozyme caused by bacteriophage；By effect
Cell membrane is different, and lysozyme can be divided into bacteria cell wall lysozyme and fungal cell wall lysozyme.
Lysozyme is capable of the cell membrane of single-minded cracking purpose bacterium.Its main attack peptide glycan, hydrolysis connection N- acetyl born of the same parents
β -1,4 glycosidic bonds of the 4th carbon atom of Teichaic acid and N-acetyl-glucosamine.Whole process is that lysozyme is tied by two first
" ditch " between structure domain is attached on peptide glycan molecule；Its subsequent substrate forms the conformation of transition state in enzyme.According to
Phillips mechanism, lysozyme are combined with glucohexaose.Then the 4th sugar distortion on glucohexaose is half chair by lysozyme
Shape conformation.In this twisted state (energy is higher), glycosidic bond is easy for being broken.Positioned at antalzyme protein sequence 35
The glutamic acid (Glu35) of position and the side chain of the aspartic acid of 52 (Asp52) are very crucial to the activity of lysozyme.Glu35 makees
For the proton donor of glycosidic bond, the C-O keys of substrate are sheared；And Asp52 participates in generation glycosyl enzyme intermediate as nucleopilic reagent.With
Afterwards, glycosyl enzyme intermediate reacts with hydrone, hydrolysis generation product, and enzyme keeps constant.
The extracting method of lysozyme includes following several：1st, direct crystallization method：This method is simple to operate, and cost is low, but can not
To separate micro lysozyme.2nd, ion exchange chromatography：This method has simplicity, efficient, cost is low, and can automate continuous
The advantages of operation, encloses.3rd, affinity chromatography：Multiple that affinity chromatography isolates and purifies is higher, quality is preferable, and can be widely applied to
The lysozyme in various sources, for concentrating and refining micro lysozyme.But because the making of affinity adsorbent is more complicated, limit
Its extensive utilization is industrially made.4th, ultrafiltration is combined with affinity chromatography：Ultrafiltration is combined extraction lysozyme with affinity chromatography,
It is the new method developed in recent years.Most of foreign protein is removed with ultrafiltration first, retains lysozyme, now lysozyme is also relatively thick,
Again purified product is obtained with affinity chromatography.5th, membrane chromatography technology：Late 1980s, there is affinity film chromatography technology.
The technology has UF membrane and affine the characteristics of separating concurrently.Compared with traditional UF membrane, affinity chromatography, membrane chromatography technology not only has
There have that purification is high, pressure drop is small, disengaging time is short, large biological molecule be denatured probability in separation process to be small, and allows comparatively fast
Charging rate the features such as, and be more easy to accomplish scale production than post affinity chromatography.6th, ultrafiltration：With traditional bio-chemistry separation skill
Art is compared, and hyperfiltration technique is a major advantage that the high yield output of product.But although hyperfiltration technique waited in reverse osmosis and concentration
It is widely used in journey, but a kind of protein stripping technique very big as the potential in biological industry field, ultrafiltration
It is not fully utilized yet.7th, reverse micelle extracts：Reverse micelle extraction is a kind of new extracting process that developed recently gets up, and it is profit
With reverse micelle that a small amount of surfactant formed is added in organic solvent come the method extracted, organic solvent can not be used for some
The bioactive substances such as the enzyme and reactive protein of extraction have opened up a kind of new isolation technics, expand organic solvent extraction
The scope of application.8th, bioseparation technology：Bioseparation technology based on affinity is (immobilized metal affinity chromatography, affine heavy
Form sediment, affinity filtration method), and one of lysozyme extracting method.
Lysozyme has a variety of unique and important pharmacological action.
First, anti-inflammation.The cell membrane of bacterium is made up of murein, and murein is by 2-Acetamido-2-deoxy-D-glucose and N-
Acetylmuramic acid replaces the polymer of composition, can be with connecting peptides, referred to as peptide glycan on cell wall residue.Polysaccharide is deposited with linear form
It can be connected with each other between polysaccharide chain located adjacent one another by peptide chain part, so as to form three-dimensional structure.Bacteriolyze enzyme specificity
The glycosidic bond that ground is acted between the -acetylmuramic acid of peptide glycan molecule and N- acetyl glucosamine ammonia, as a result makes bacteria cell wall
Become loose, lose the protective effect to cell, last cell dissolving is dead；Lysozyme can directly kill gram positive bacteria,
Under the participation of S-IgA complement, moreover it is possible to kill gram-negative bacteria：Such as ETEC.Lysozyme can also with it is each
The acidic materials that kind induces inflammation combine, and reduce bacterial endotoxin release, so as to mitigate endotoxemia, slow down inflammation and send out
Raw process, and certain synergistic effect is known as to antibiosis, improve glutinous more glycometabolisms of periplast, play anti-inflammation, reparation group
The effect knitted.Tests prove that being coupled human lysozyme and proteolytic enzyme with a kind of cellulose copolymer, it is remarkably improved molten
Bacterium enzyme antibacterial ability, accelerating wound healing.
Second, it is antiviral.Lysozyme carries a large amount of positive charges under neutral fluid environment, can be with the virus with negative electrical charge
Albumen directly acts on, and is combined to form double salt with DNA, RNA, desorption base albumen, makes virally inactivated.By herpesvirus infection
Have in Hela cell culture fluids plus after lysozyme and suppress cell degeneration effect.Lysozyme can also suppress adenovirus growth, can be used for
The viral illness treatment such as herpes zoster, parotitis, chicken varicella, hepatitis and influenza.
3rd, strengthen immunity.Lysozyme participates in panimmunity reaction in body, in body normal defense and non-specificity
Play an important roll in immune.It can improve and strengthen macrophage phagocytosis digestive function, reduce white caused by cytostatics
Leukopenia, with reference to bacteria lipopolysaccharide, mitigate endotoxin effect, to reach the purpose of enhancing Abwehrkraft des Koepers.Lysozyme is in itself
With t cell epitope, 2 types (Th2) t helper cell can be induced to react.Oral lysozyme energy inducing mouse produce systemic Th1 and
Th2 immune responses, strengthen immunologic function, therefore, oral lysozyme has the control infection of the upper respiratory tract and treats the function of dysentery.
In addition, lysozyme, which also has, activates hematoblastic function, tissue local blood circulation disorder can be improved, decompose fester, enhancing office
Portion's defense function, so as to embody its hemostasis, detumescence and the repair for accelerating body injury tissue.It is alternatively arranged as a kind of host
Resistance factor, tissue local is shielded.
Lysozyme kills in food fresh keeping anti-corrosion, oral cavity disinfecting, Water Disinfection, air purification and sterilizing and household electrical appliance
It is widely used in the fields such as bacterium sterilization.Research finds that lysozyme can be used for preservative of chilled meat, and the antistaling agent uses completely
Safe and nontoxic, crude scientifically with being combined into, can extend 1-4 times of the shelf-life of Chilled Meats.The antistaling agent can be with resistance to
By the temperature below 95 DEG C, and retention properties is stable.Lysozyme can also be used for the anti-corrosion of dairy products, add during cheese processing
A certain amount of lysozyme is added to prevent bubbling, being spoiled for middle and later periods cheese；Cheese base in cheese ageing process can also be kept
The quality of liquid, it is often more important that it can play long-term bactericidal action, effectively control the fermentation of butyric acid.In addition, bacteriolyze
Enzyme can also be used for sterilizing of the anti-bacterial refreshing of food flexible packing material, the anti-corrosion of wine and beverage, aquatic products etc..
Bacterium in air is adsorbed on dirt particle, and common pathogenic bacteria have Mycobacterium tuberculosis, Corynebacterium diphtheriae bar
Bacterium, bordetella pertussis, staphylococcus aureus, micrococcus scarlatinae, Neisseria meningitidis, Legionella etc., breathing can be caused
Road transmission disease or wound infection.In view of safety factor, the filter such as generally use glass fibre HEPA filters to air
Purify degerming.However, microorganism easily breeds on HEPA, dispersed, secondary pollution is formed.Domestic someone has studied successfully one kind
Lysozyme PPHEPA filters, lysozyme is immobilized on HEPA whole filter paper fibre by it, can be caught and be killed in fibrous inside
Microorganism killing, it effectively prevent secondary pollution.
Health type household electrical appliance are the trend and trend of current household electric appliances.It is applied to the health of white goods industry at present
Sterilization technology using tradition and physical sterilization and chemical disinfection, there are potential safety hazard, secondary pollution problems more.Will be molten
The sterilization that bacterium enzyme is applied to white goods industry can solve the problems, such as this respect well.Lysozyme sterilization technology is in sky at present
Gas cleaner, central air-conditioning, there is tentative application on purifier.
Bacterium is the main reason for mouth disease occurs, and dental caries, pulpitis, periapical inflammation and part mucous membrane disease are all and oral cavity
The infection of bacterium is inseparable.Lysozyme is antimicrobial component important in people's saliva, participates in nonimmune defence of the host to bacterium,
The homeostasis of oral environment is adjusted, the occurrence and development with resisting mouth disease have certain relation.Lysozyme can suppress oral cavity
The growth of carious tooth, suppress thalline glycolysis and production acid, so most scholars think that lysozyme has certain effect to pre- anti-caries.This
Outside, lysozyme can also treat canker sore, while can also can also be used as cleaning agent for mouth cavity, take care of one's teeth and oral health.
The healing of canker sore with repair tissue, can be advantageous in oral cavity using lysozyme.Staphylococcus lysozyme and synergist, stabilizer
Mouth cleaning solution is may also be fabricated which, several pathogenic bacteria for causing mouth disease, such as staphylococcus, hemolytic streptococcus, white are read
Pearl bacterium, anaerobic bacteria etc. have inhibitory or killing effect.By detection, sterilizing rate is up to 99.9% in 5min.Acute toxicity test, cumulative toxicity examination
Test, the safety evaluatio such as micronucleus test, mucosa irritation test meets the requirements.Extracorporeal disinfecting, which is tested, to be shown, the compound system of lysozyme
Agent can effectively suppress and kill pars oralis pharyngis pathogenic bacteria, can effectively treat the mouth diseases such as acute/chronic pharyngitis, tonsillitis.Due to
Treatment and prevention of the lysozyme to mouth disease have the effect of fine, existing lysozyme lozenge listing domestic at present, lysozyme
There is very wide prospect in terms of mouth disease.
Gynura procumbens (Lour.) Merr (Gynura procumbens (Lour.) Merr) is composite family pseudo-ginseng platymiscium, and perennial herb is planted
Thing, alias procumber velvetplant herb, tree pseudo-ginseng, stone pseudo-ginseng etc..Draft is climbed up by holding on to, there is foul smell, stem is crawled, filbert or purple, there is bar rib, hairless
Or have pubescence when young, there is branch；Leaf has handle；Blade ovate Long Circle or ellipse, it is long 3~8 centimetres, it is wide 1.5~3.5 centimetres；
Basidixed or axillary corymb, each corymb have 3~5 capitulums；The narrow mitriform of involucre or funnel-form, base portion have 5~6
The linear squamella of piece, each capitulum have little Hua 20~30, and pattern is orange；The long 12-15 millimeters of corolla, pipe portion is thin, long 8-
10 millimeters, top expands, sliver ovum shape lanceolar, top point；Achene cylinder, long 4~6 millimeters, Chestnut.The place of production is distributed in
The ground such as Guangdong, Hainan, Guizhou, Yunnan, often it is born in woodland, by small stream, on the sand in hillside fields, climbs up by holding on on shrub or arbor.
Vietnam, Thailand, Indonesia and Africa are also distributed.
Gynura procumbens (Lour.) Merr young stem and leaf is nutritious, its protein, amino acid, total reducing sugar, fat content be respectively 1.24%,
0.87%th, 1.5%, 0.79%, dietary fiber content is 2.40 grams/100 grams, and Vitamin C content is 11.2 milligrams/100 grams, rich
It is the pollution-free food that the elderly replenishes the calcium, also containing flavonoids, triterpene aglycon class, phytosterin compound and perfume (or spice) containing organic calcium
The phenolic acid compounds such as oxalaldehyde, syringaldehyde, P-hydroxybenzoic acid, hydroxycinnamic acid, protocatechuic acid.
Gynura procumbens (Lour.) Merr is a kind of unique plant of dietotherapeutic, and it is young stem and leaf that it, which mainly eats part,.It is with present people
Edible curled mallow belong to together, food flavor is soft and smooth, and delicate fragrance is tasty, can fry, be cold and dressed with sauce, float soup, and dumpling, steamed stuffed bun are done after the chopping of its cauline leaf
Filling have much fragrance, for brew meal, its taste it is further preferred that.Its leaf is also raw-eaten, or takes fresh leaf to be brewed with boiling water and work as tea-drinking, such as will
Blade is drunk after drying for soaked, then is even more not have some flavor.
Gynura procumbens (Lour.) Merr whole body is all precious, and rhizome is the excellent raw material of Chinese patent drug, and its slightly sweet flavor is mild-natured, and can stimulate the menstrual flow work
Network, inflammation-diminishing and cough-controlling, scattered silt detumescence, blood activating and promoting tissue regeneration.Cook edible or medicinal, there is reducing blood lipid, hypotensive, hypoglycemic effect, also
Replenish the calcium, anti influenza and hepatitis B, anti-flu, improve body immunity and other effects, external application to herpes zoster, various dermatitis,
Scald, burn and nameless gall have the effect of good, can also stop blooding, relieve pain, be antipruritic, skin care moisturizing and anti-aging.
At present, lysozyme is combined with Gynura procumbens (Lour.) Merr for preventing and treating odontopathy, particularly gingivitis and bacterial plaque
Using there is not been reported.
The content of the invention
The technical problems to be solved by the invention, which are to provide one kind, can effectively prevent and treat gingivitis and bacterial plaque, have no toxic side effect
And drug resistance, the natural feature toothpaste in active component source.
The present invention solves the above problems the technical scheme adopted is that a kind of ointment containing lysozyme is provided, wherein living
Property composition lysozyme and lie down chrysanthemum Notogineng Extract weight ratio be (1:10)~(10:1).
Preferably, in the toothpaste, lysozyme and the weight ratio for lying down chrysanthemum Notogineng Extract are (1:5)~(5:1).
It is furthermore preferred that lysozyme and the weight ratio for lying down chrysanthemum Notogineng Extract are 1:4.
Preferably, the Gynura procumbens (Lour.) Merr extract is the ethanol extract of Gynura procumbens (Lour.) Merr stem.
Preferably, the toothpaste further comprises one kind or more in rubbing agent, NMF, adhesive, sweetener, water
Kind.
It is furthermore preferred that one or more of the rubbing agent in calcium monohydrogen phosphate, silica etc.；The NMF choosing
One or more from sorbierite, propane diols etc.；Described adhesive is selected from carragheen, konjac glucomannan, sodium carboxymethylcellulose etc.
In one or more；The sweetener is saccharin sodium.
Preferably, in the toothpaste of 100 parts by weight, the lysozyme containing 0.5-5 weight % and Gynura procumbens (Lour.) Merr extraction
Thing.
The present invention also provides a kind of application of above-mentioned lysozyme toothpaste in the toothpaste of preventing and treating gingivitis or bacterial plaque is prepared.
The invention has the advantages that：
It is surprising that by repeated tests, present inventors have unexpectedly found that lysozyme and Chinese herbal medicine are lieed down into chrysanthemum three
Seven extract combinations use effect of the preventing and treating with Synergistic for gingivitis and bacterial plaque, coordinate other active components to make
With better.
Compared with common Chinese herbal toothpaste, lysozyme toothpaste preparation method of the invention is simple, and compatibility is reasonable, two kinds of activity
For composition while respective effect is played, and can produces synergistic function, significantly prevents and treats oral cavity gingivitis and bacterial plaque.Bacteriolyze
Enzyme and Gynura procumbens (Lour.) Merr source are natural, safe and non-toxic, have no side effect；Because, without western medicine composition, therefore toothpaste is without resistance in toothpaste
Property.
Embodiment
The present invention is further described with reference to embodiment, but the implementation of the present invention is not limited to this.Under
State experimental method used in embodiment unless otherwise specified, be conventional method.
The extraction of embodiment 1, Gynura procumbens (Lour.) Merr
After Gynura procumbens (Lour.) Merr stem powder is crossed into 40 mesh sieves, with petroleum ether decolouring degreasing 3h, again with 30 after drying to constant weight
90% ethanol of times weight 2 hours every time, merges extract solution, produced after concentrate drying in 80 DEG C of heating and refluxing extractions 3 times.
The preparation of embodiment 2, lysozyme/Gynura procumbens (Lour.) Merr extract toothpaste
Take 5 parts by weight Gynura procumbens (Lour.) Merr extract as made from embodiment 1, the silica of 50 parts by weight, 20 parts by weight
Sorbierite, the carragheen of 1 parts by weight, the water mixing of the saccharin sodiums of 0.2 parts by weight and surplus, containing for 100 parts by weight is made
The toothpaste A1 of Gynura procumbens (Lour.) Merr extract.
Take the lysozyme of 5 parts by weight, the silica of 50 parts by weight, the sorbierite of 20 parts by weight, the OK a karaoke club of 1 parts by weight
Glue, the saccharin sodium of 0.2 parts by weight and the water mixing of surplus, the toothpaste A2 containing lysozyme of 100 parts by weight is made.
Take the lysozyme of 4.167 parts by weight, the Gynura procumbens (Lour.) Merr extract as made from embodiment 1 of 0.833 parts by weight, 50
The silica of parts by weight, the sorbierite of 20 parts by weight, the carragheen of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water of surplus
Mixing, be made 100 parts by weight containing lysozyme and lie down chrysanthemum Notogineng Extract (weight ratio be 5:1) toothpaste A3.
Take the lysozyme of 2.5 parts by weight, the Gynura procumbens (Lour.) Merr extract as made from embodiment 1 of 2.5 parts by weight, 50 weight
The silica of part, the sorbierite of 20 parts by weight, the carragheen of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water of surplus mix
Close, be made 100 parts by weight containing lysozyme and lie down chrysanthemum Notogineng Extract (weight ratio be 1:1) toothpaste A4.
Take the lysozyme of 1 parts by weight, the Gynura procumbens (Lour.) Merr extract as made from embodiment 1 of 4 parts by weight, 50 parts by weight
Silica, the sorbierite of 20 parts by weight, the carragheen of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water mixing of surplus, system
Into 100 parts by weight, containing lysozyme, (weight ratio is 1 with chrysanthemum Notogineng Extract is lieed down:4) toothpaste A5.
Take the lysozyme of 0.833 parts by weight, the Gynura procumbens (Lour.) Merr extract as made from embodiment 1 of 4.167 parts by weight, 50
The silica of parts by weight, the sorbierite of 20 parts by weight, the carragheen of 1 parts by weight, the saccharin sodium of 0.2 parts by weight and the water of surplus
Mixing, be made 100 parts by weight containing lysozyme and lie down chrysanthemum Notogineng Extract (weight ratio be 1:5) toothpaste A6.
The application of embodiment 3, the toothpaste comprising lysozyme/Gynura procumbens (Lour.) Merr extract in preventing and treating gingivitis and bacterial plaque
3.1 research object
Double blinding, layering, the parallel clinical research carried out to the adult men and women volunteer of health.First from researcher to experiment
Object makes experiment purpose, research details, the responsibility for participating in research clear.After the subject for being ready to participate in fills in voluntary book, by grinding
The person of studying carefully checked it, is judged with exclusion standard according to including, is screened tested crowd.Preliminary screening process includes soft group of oral cavity
Knit and sclerous tissues checks, oulitis and bacterial plaque inspection are so that whether being selected in for experimental subjects determined.Finally select improvement Quigley-
210 research objects of Hein plaque index >=1.5 and Loe-Silness index of gingivitis >=1.0 have participated in research, and according to
Plaque index and gingival index are divided into 7 balance groups, and every group of 30 people, men and women half and half, and experimental subjects the range of age is 20~40 years old.
3.2 inspection method
Supragingival plaque is evaluated using the Turesky plaque indexs of Quigley-Hein improvement.To supragingival plaque dyeing and root
According to following standard recording：
0=is without bacterial plaque；
The bacterial plaque that 1=necks portion marginal existence is dispersed in；
The visible continuous thin bacterial plaque band (wide up to lmm) in 2=necks edge；
3=necks portion bacterial plaque band is more than lmm, but less than the 1/3 of facing；
The 1/3~2/3 of 4=bacterial plaques covering facing；
5=bacterial plaques cover facing more than 2/3.
Tooth detection buccal surface and lingual surface, it is 10 for the maximum numeration of each tooth.Except third molar, artificial tooth and there is neck reparation
The tooth of body, remaining tooth is in the row of numeration.The mean of each subject's plaque index is succeeded in one's scheme by personal whole tooth detections
Number sum (each 2 records of tooth) divided by total measurement number (teeth number is multiplied by 2) obtain.
Gingivitis measures gum using improvement Loe-Silness index of gingivitis measurements before dyeing measures plaque index
Situation.Gingivitis method of counting and standard are as follows：
0=is without oulitis；
Oulitis that 1=is slight, gum color and luster and quality are slightly changed, but are visited and examined no bleeding；
2=moderate inflammations, gum are bright.Red, swollen, bleeding is examined in hyperplasia, spy；
3=hyperphlogosises, the obvious rubescent, hyperplasia of gum, there is hematostaxis.
As the inspection of plaque index, each tooth inspection record buccal surface and lingual surface, the maximum numeration of a tooth is 6.Remove
Third molar, artificial tooth and the tooth for having neck dummy, remaining tooth are included in the row of numeration.Loe-Silness index of gingivitis
Mean refer to the summation (each face of tooth 2) divided by total measuring surface number (tooth of measurement multiplies 2) institute's total of all tooth counter values
Value.
3.3 experimental methods and result
After baseline inspection (soft, sclerous tissues, bacterial plaque, gingivitis) is completed, all experimentss object receives to clean one's teeth and according to bacterium
Spot and oulitis situation are divided into 7 balance groups and participate in the research.Random 6 experimental groups being assigned as using the toothpaste of embodiment 2
(A1-A6) and 1 control group (without any active component, the auxiliary material in remaining toothpaste is identical with A1-A6, and excess water complements to 100
Parts by weight).
Subject is using unified soft toothbrush and uses according to regulations toothpaste, it is desirable to brushes teeth within 1st 2 times (early, evenings), every time
1min.It is required that any other dental care product such as other toothpaste or toothbrush, gargle, dental floss are not had in experimentation.It is real
Good balance is carried out before testing from number, age, sex, bacterial plaque and index of gingivitis to experimental subjects to be grouped, nothing between each group
Statistics difference.When using toothpaste 6 months, subject's bacterial plaque and gingivitis situation are assessed according to the method described above, 6
Statistical result after individual month after clinical examination is shown in Table 1.
The plaque index and index of gingivitis of table each experimental group subject after 16 months
| Plaque index | Index of gingivitis | |
| Control group | 2.79 | 1.87 |
| A1 experimental groups (Gynura procumbens (Lour.) Merr extract) | 2.41 | 1.55 |
| A2 experimental groups (lysozyme) | 2.50 | 1.68 |
| A3 experimental groups (5:1 lysozyme and prostrate chrysanthemum Notogineng Extract) | 2.05 | 1.03 |
| A4 experimental groups (1:1 lysozyme and prostrate chrysanthemum Notogineng Extract) | 1.72 | 1.05 |
| A5 experimental groups (1:4 lysozyme and prostrate chrysanthemum Notogineng Extract) | 1.51 | 0.71 |
| A6 experimental groups (1:5 lysozyme and prostrate chrysanthemum Notogineng Extract) | 1.98 | 0.99 |
The lysozyme toothpaste of the present invention can effectively prevent and treat gingivitis and tooth it can be seen from the contrast experiment of embodiment 3
Bacterial plaque, particularly lysozyme and being applied in combination for chrysanthemum Notogineng Extract of prostrate generate synergy, reduce active component
Usage amount.In the case where active component total amount keeps constant, using lysozyme of the present invention and chrysanthemum Notogineng Extract is lieed down
The index of gingivitis of toothpaste (the A1 and A2) subject of lysozyme or Gynura procumbens (Lour.) Merr extract is relatively used alone in toothpaste (A3-A6)
There is different degrees of reduction with plaque index, it was demonstrated that effect of the toothpaste of the present invention in terms of preventing and treating gingivitis and bacterial plaque shows
Write, wherein lysozyme and Gynura procumbens (Lour.) Merr extract weight ratio are 1:4 toothpaste effect is the most prominent, and plaque index and oulitis refer to
Number is only respectively 1.51 and 0.71, is significantly higher than control group, generates and be difficult to expected excellent effect.
Claims (5)
1. a kind of toothpaste containing lysozyme, it is characterised in that wherein active component lysozyme and prostrate chrysanthemum Notogineng Extract
Weight ratio is 1:4, the preparation method of the Gynura procumbens (Lour.) Merr extract is：After Gynura procumbens (Lour.) Merr stem powder is crossed into 40 mesh sieves,
With petroleum ether decolouring degreasing 3h, again with 90% ethanol of 30 times of weight in 80 DEG C of heating and refluxing extractions 3 times after drying to constant weight, often
Secondary 2 hours, merge extract solution, produced after concentrate drying.
2. toothpaste according to claim 1, it is characterised in that toothpaste further comprises rubbing agent, NMF, bonding
One or more in agent, sweetener, water.
3. toothpaste according to claim 2, it is characterised in that the rubbing agent is in calcium monohydrogen phosphate, silica
It is one or more；One or more of the NMF in sorbierite, propane diols；Described adhesive is selected from carragheen, evil spirit
One or more in taro glue, sodium carboxymethylcellulose；The sweetener is saccharin sodium.
4. the toothpaste according to right wants 1, it is characterised in that in the toothpaste of 100 parts by weight, contain 0.5-5 weight %
Lysozyme and lie down chrysanthemum Notogineng Extract.
5. the toothpaste containing lysozyme described in claim any one of 1-4 is in the toothpaste of preventing and treating gingivitis or bacterial plaque is prepared
Application.
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| EP3192495A1 (en) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Oral care compositions |
| CN105943408A (en) * | 2016-06-07 | 2016-09-21 | 上海舒雅心日用品有限公司 | Fluoride-free anti-infection toothpaste with natural sterilization effect and preparation method thereof |
| KR101899237B1 (en) * | 2017-02-27 | 2018-09-14 | 김동환 | Organic acid composition to be added to antimicrobial toothpaste and method for producing the same |
| CN107714611A (en) * | 2017-11-13 | 2018-02-23 | 常州麒通国际贸易有限公司 | A kind of edible toothpaste for children |
| CN110237241A (en) * | 2019-07-05 | 2019-09-17 | 广州康云生物科技有限公司 | A kind of compositions of additives containing lysozyme and plant extracts |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101036628A (en) * | 2007-04-08 | 2007-09-19 | 谢平 | Gynura procumbens medical toothpaste |
| CN101721324A (en) * | 2010-01-14 | 2010-06-09 | 江苏雪豹日化有限公司 | Biological complex enzyme toothpaste and preparation method thereof |
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| JPS5254037A (en) * | 1975-10-28 | 1977-05-02 | Sunstar Inc | Lysozymeeblended dentifrice composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101036628A (en) * | 2007-04-08 | 2007-09-19 | 谢平 | Gynura procumbens medical toothpaste |
| CN101721324A (en) * | 2010-01-14 | 2010-06-09 | 江苏雪豹日化有限公司 | Biological complex enzyme toothpaste and preparation method thereof |
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Denomination of invention: A toothpaste containing lysozyme Effective date of registration: 20210630 Granted publication date: 20180223 Pledgee: Agricultural Bank of China Limited by Share Ltd. Pujiang County branch Pledgor: ZHEJIANG AEGIS BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. Registration number: Y2021330000759 |
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