CN110237241A - A kind of compositions of additives containing lysozyme and plant extracts - Google Patents
A kind of compositions of additives containing lysozyme and plant extracts Download PDFInfo
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- CN110237241A CN110237241A CN201910602895.9A CN201910602895A CN110237241A CN 110237241 A CN110237241 A CN 110237241A CN 201910602895 A CN201910602895 A CN 201910602895A CN 110237241 A CN110237241 A CN 110237241A
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/287—Chrysanthemum, e.g. daisy
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- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/539—Scutellaria (skullcap)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
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Abstract
The invention discloses a kind of compositions of additives containing lysozyme and plant extracts.The compositions of additives is made of lysozyme and plant extracts；The plant extracts is one of Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tea extract, glycyrrhiza glabra root extract, german chamomile flower extract, rosemary leaf extract or a variety of.The present invention is by one of lysozyme and above-mentioned plant extracts or a variety of compound uses, on the one hand it solves and be easy to cause oral bacteria ecological disturbance commonly using the oral care product of chemical component, make it is bacterial resistance occurred, cannot achieve the effect that prevent mouth disease the problem of；On the other hand, the lysozyme deficiency weaker to Gram-negative bacteria sterilizing ability is compensated for, while compensating for the deficiency for being applied alone plant extracts Antifungal activity weak, makes the compositions of additives containing lysozyme and plant extracts that there is good wide spectrum to press down bactericidal effect.
Description
Technical field
The present invention relates to lysozyme applied technical fields, and in particular, to a kind of containing lysozyme and plant extracts
Compositions of additives.
Background technique
Oral problem mainly as caused by bacterium, is arranged using effective toothpaste, gargle and oral disease therapeutic etc.
The quantity that can be substantially reduced pathogenic bacteria in oral cavity is applied, improvement even is eliminated mouth disease.The oral cavities such as toothpaste, oral nursing liquid shield
It is mainly the active constituent or antipathogenic composition leaned in product why reason product, which is able to achieve these functions, these antipathogenic compositions are mostly
Chemical classes bacteriostatic agent (such as triclosan, Cetylpyridinium Chloride, chlorohexidene, antibiotic) is protected commonly using the oral cavity containing these ingredients
Reason product be easy to cause Oral health behaviours to lack of proper care, or make it is bacterial resistance occurred, cannot achieve the effect that prevent mouth disease, instead
And cause other multiple oral diseases.
Lysozyme (Lysozyme) is the non-specific material composition of one of saliva, can be suppressed growth of microorganism, with
Antibiotic or the comparison of other antibacterial substances, it is not in drug resistance that lysozyme, which is used for a long time,.Lysozyme can effectively hydrolytic bacteria
The peptide glycan of cell wall, hydrolytic sites are 1 carbon atom and N-Acetyl-D-glucosamine (NAG) of -acetylmuramic acid (NAM)
4 carbon atoms between β -1,4 glycosidic bond.Peptide glycan is the main ingredient of bacteria cell wall, it is by NAM, NAG and peptide " tail "
(usually 4 amino acid) composition, NAM are connected with NAG by β-Isosorbide-5-Nitrae glycosidic bond, and peptide " tail " is connected by D- lactoyl carboxyl
On the 3rd carbon atom of NAM, connected between peptide tail by peptide " bridge " (peptide bond or a few amino acid), NAM, NAG, peptide
" tail " and peptide " bridge " has collectively constituted the multilayer network structure of peptide glycan, any in above structure as the skeleton of cell wall
Chemical bond rupture can all lead to the damage of bacteria cell wall.For gram-positive bacteria (G+), such as staphylococcus aureus, withered
Straw bacterium or micrococcus lysodeikticus etc., with Gram-negative bacteria (G-), as Escherichia coli, Fusobacterium nucleatum, porphyromonas gingivalis,
Pneumobacillus etc., peptidoglycan content is different in cell wall.G+Bacteria cell wall almost all is made of peptide glycan, and G-Bacterium
Only inner wall layer is peptide glycan, and therefore, lysozyme is to G+Bacterium, which has to press down well, kills effect, but to compared with G-The antibacterial work of bacterium
With weaker.
Plant extracts, which refers to, to be present in plant, and people have life by what the method for physics or chemistry extracted
The active substance of object, effective component such as alkaloid, volatile oil, flavones, polysaccharide, organic acid, tannin, grease etc. all have difference
The antibacterial action of degree.But the Antifungal activity of most plants extract is relatively low, and fungistatic effect is unobvious when low dose uses, greatly
In the presence of the quality for influencing food when dosage uses, such as the problems such as mouthfeel, color, smell.It has had not yet to see lysozyme and has planted
Object extract compounds the relevant report for inhibiting oral bacteria.
Summary of the invention
The purpose of the invention is to overcome the above-mentioned deficiency of the prior art, one kind is provided and contains lysozyme and plant extract
The compositions of additives of object.
Another object of the present invention is to provide above-mentioned compositions of additives to have the oral care of bacteriostasis efficacy in preparation
Application in articles.
To achieve the goals above, the present invention is achieved by following scheme:
A kind of compositions of additives containing lysozyme and plant extracts, the compositions of additives is by lysozyme and plant
Object extract composition；The plant extracts is Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tealeaves extraction
One of object, glycyrrhiza glabra root extract, german chamomile flower extract, rosemary leaf extract are a variety of.
On the one hand the present invention is by solving one of lysozyme and above-mentioned plant extracts or a variety of compound uses
Be easy to cause oral bacteria ecological disturbance commonly using the oral care product of chemical component, make it is bacterial resistance occurred, cannot
Achieve the effect that the problem of preventing mouth disease；On the other hand, significant synergy is produced, it is blue to leather to compensate for lysozyme
The weaker deficiency of family name's negative bacterium sterilizing ability, while the deficiency for being applied alone plant extracts Antifungal activity weak is compensated for, make containing molten
There is the compositions of additives of bacterium enzyme and plant extracts good wide spectrum to press down bactericidal effect.
Preferably, when plant extracts is a kind of, the mass ratio of the lysozyme and plant extracts be (0.01~
10): (0.1~10)；
When plant extracts is two kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10):
(0.1~10): (0.1~10)；
When plant extracts is three kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10):
(0.1~10): (0.1~10): (0.1~10)；
When plant extracts is four kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10):
(0.1~10): (0.1~10): (0.1~10): (0.1~10)；
When plant extracts is five kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10):
(0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10)；
When plant extracts is six kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10):
(0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10)；
When plant extracts is seven kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10):
(0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10).
It is highly preferred that when plant extracts is Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tealeaves mention
When taking object, glycyrrhiza glabra root extract, german chamomile flower extract, any in rosemary leaf extract, the lysozyme and plant
The mass ratio of object extract is 1:2.
It is highly preferred that when plant extracts is Gotu Kola P.E and polygonum cuspidate root extract, the lysozyme and each plant
The mass ratio of object extract is 1:1:1.
It is highly preferred that when plant extracts is Gotu Kola P.E, polygonum cuspidate root extract and radix scutellariae root extract, it is described
The mass ratio of lysozyme and each plant extracts is 1:0.66:0.66:0.66.
It is highly preferred that when plant extracts is that Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract and tealeaves mention
When taking object, the mass ratio of the lysozyme and each plant extracts is 1:0.5:0.5:0.5:0.5.
It is highly preferred that when plant extracts is Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tealeaves mention
When taking object and glycyrrhiza glabra root extract, the mass ratio of the lysozyme and each plant extracts is 1:0.4:0.4:0.4:0.4:
0.4。
It is highly preferred that when plant extracts is Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tealeaves mention
When taking object, glycyrrhiza glabra root extract and german chamomile flower extract, the mass ratio of the lysozyme and each plant extracts is 1:
0.33:0.33:0.33:0.33:0.33:0.33.
It is highly preferred that when plant extracts is Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tealeaves mention
When taking object, glycyrrhiza glabra root extract, german chamomile flower extract and rosemary leaf extract, the lysozyme and each plant extracts
Mass ratio be 1:0.17:0.17:0.5:0.17:0.5:0.17:0.33.
Compositions of additives provided by the present invention containing lysozyme and plant extracts, which can be used for institute, in need to be made
Use the technical field of lysozyme, such as food, medicine, feed, dental products technical field.
The inventors discovered that the compositions of additives containing lysozyme and plant extracts is added to oral care product
In toothpaste, oral nursing liquid, there is gained oral care product good wide spectrum to press down bactericidal effect, and then reaches and inhibit oral cavity
The purpose of bacteria breed, while having effects that treat gingivitis and bleeding gums.
Therefore, the oral care implement that the compositions of additives has bacteriostasis efficacy in preparation is also claimed in the present invention
In application.
Preferably, the oral care implement is toothpaste, oral nursing liquid or gel.
Compared with prior art, the invention has the following advantages:
(1) present invention by lysozyme and Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tea extract,
One of glycyrrhiza glabra root extract, german chamomile flower extract, rosemary leaf extract or a variety of combined uses solve often
It is be easy to cause oral bacteria ecological disturbance using the oral care product of chemical component, makes bacterial resistance occurred, cannot be reached
The problem of preventing the effect of mouth disease.
(2) it is used in combination, is produced significant using one of plant extracts of the present invention or a variety of and lysozyme
Synergy, compensate for the lysozyme deficiency weaker to Gram-negative bacteria sterilizing ability, while compensating for and plant is applied alone to mention
The deficiency for taking object Antifungal activity weak makes the compositions of additives containing lysozyme and plant extracts that there is the suppression of good wide spectrum to kill
Bacterium effect.
(3) oral care product such as toothpaste, mouth are made into using the compositions of additives containing lysozyme and plant extracts
There is good wide spectrum to press down bactericidal effect for chamber conditioning liquid, gained oral care product, and then reaches and inhibit oral bacteria growth
Purpose, while having effects that treat gingivitis and bleeding gums.
Specific embodiment
The present invention is made combined with specific embodiments below and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method as used in the following examples is normal unless otherwise specified
Rule method；Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
Embodiment 1
One kind containing lysozyme and plant extracts (Gotu Kola P.E, polygonum cuspidate root extract, radix scutellariae root extract, tea
One of leaf extract, glycyrrhiza glabra root extract, german chamomile flower extract, rosemary leaf extract are a variety of) compound,
According to the evaluation method of QB 2738-2012 daily chemical products antibacterial bacteriostatic effect, compositions of additives or raw material monomer object pair are measured
Common pathogen Streptococcus mutans (G in oral cavity-), porphyromonas gingivalis (G-), staphylococcus aureus (G+), Escherichia coli
(G-) inhibiting effect.
1, the fungistatic effect of raw material monomer is studied, formula is any one formula in the formula 1~11 in table 1.
The composition and fungistatic effect of the formula of table 1 1~11
As can be seen from Table 1, lysozyme kills effect than Gram-negative bacteria to the suppression of gram-positive bacteria and gets well, and
And the concentration of lysozyme has just had relatively better effect when reaching 1%；7 Plant Extracts have one at antibacterial aspect
Determine broad-spectrum antibacterial effect, but even if effect is also less desirable when reaching 2% concentration.
2, the fungistatic effect of the compound of lysozyme and single plant extracts is studied, formula is the formula 12 in table 2
Any one in~18 is formulated.
The composition and fungistatic effect of the formula of table 2 12~18
As can be seen from Table 2: antibacterial effect when after (1) lysozyme and single plant extracts compounding than raw material monomer
Fruit will get well, lysozyme and plant extracts it is compound after have cooperative synergism effect and influence each other without product and lead to the activity of enzyme
The case where failure and the failure of plant extracts activity；(2) fungistatic effect of lysozyme+radix scutellariae root extract compound is compared with other
Compound to get well, followed by lysozyme+glycyrrhiza glabra root extract compound, lysozyme+rosemary leaf extract are answered
Closing object also has preferable fungistatic effect.
3, the fungistatic effect of the compound of lysozyme and multiple plant extracts is studied, formula is the formula 19 in table 3
Any one in~25 is formulated.
The composition and fungistatic effect of the formula of table 3 19~25
As can be seen from Table 3, in the case where lysozyme and plant extracts equivalent add, the bacteriostasis rate of compound
As the type that plant extracts adds is more, fungistatic effect is better, when reaching+seven Plant Extracts of lysozyme while adding
When, compound bacteriostasis rate reaches highest.
4, the fungistatic effect of the compound of the different proportion of lysozyme and seven Plant Extracts is studied, each formula wraps
+ seven Plant Extracts of lysozyme, wherein bacteriolyze enzyme dosage is fixed as 1%, and total dosage of seven Plant Extracts is
2%, but the ratio in each formula between seven Plant Extracts is different, and specific formula is shown in Table the formula 26~32 in 4.
The composition and fungistatic effect of the formula of table 4 26~32
As can be seen from Table 4, in the case where it is 1% that lysozyme, which is fixed on dosage, seven Plant Extracts are different
Different fungistatic effects is had when ratio, it can be seen from the results above that formula 30 (Gotu Kola P.E: polygonum cuspidate root extract:
Radix scutellariae root extract: tea extract: glycyrrhiza glabra root extract: german chamomile flower extract: rosemary leaf extract=1:1:3:
1:3:1:2) there is a best bacteriostasis, be followed by formulated 27 (Gotu Kola P.E: polygonum cuspidate root extract: radix scutellariae root extract:
Tea extract: glycyrrhiza glabra root extract: german chamomile flower extract: rosemary leaf extract=1:1:2:1:2:1:2) also have compared with
Good bacteriostasis.
Embodiment 2
According to embodiment 1 as a result, the compound of+seven Plant Extracts of lysozyme is applied in toothpaste.
A kind of toothpaste of the compositions of additives containing lysozyme and plant extracts, the specific formula of toothpaste are shown in Table 5 and match
Side 33~39.
Preparation process is as follows:
Moisturizer (sorbierite, polyethylene glycol, propylene glycol, glycerol), rubbing agent (silica, calcium carbonate, phosphoric acid hydrogen is added
Calcium, aluminium hydroxide), adhesive (sodium carboxymethylcellulose, xanthan gum, carragheen), water, foaming agent (lauryl sodium sulfate, the moon
Osmanthus acylsarcosine sodium, Cocoamidopropyl betaine), after sweetener (saccharin sodium, Sucralose), keep vacuum degree-
0.090Mpa~-0.096Mpa stirs 20~50min；Then edible essence, lysozyme, plant extracts is added (in seven kinds
It is one or more of), it keeps vacuum degree in -0.090Mpa~-0.096Mpa, stirs 10~50min, lotion is made.
According to the evaluation method of QB 2738-2012 daily chemical products antibacterial bacteriostatic effect, measurement 33~39 gained toothpaste of formula
To Streptococcus mutans, porphyromonas gingivalis, staphylococcus aureus, Escherichia coli inhibiting effect.Measurement result such as 5 institute of table
Show.
The composition and fungistatic effect of the formula of table 5 33~39
As can be seen from Table 5: (1) being made into using the lysozyme of raw material monomer, Gotu Kola P.E, radix scutellariae root extract
Lotion, fungistatic effect are not satisfactory, wherein monomer lysozyme enzyme is slightly good, and to gram-positive bacteria golden yellow grape
Coccus has preferable inhibitory or killing effect, slightly poor to Gram-negative bacteria inhibitory or killing effect, and the Gotu Kola P.E of monomer, scutellariae,radix mention
Take object totality fungistatic effect low compared with monomer lysozyme；(2) cream being made into using the compound of lysozyme compound botanical extract
Body, compared with to get well using raw material monomer lysozyme and plant extracts, fungistatic effect arranges suitable fungistatic effect from big to small
Sequence is+two Plant Extracts of+three Plant Extracts > lysozyme of+seven Plant Extracts > lysozyme of lysozyme, wherein
When bacteriolyze enzyme dosage is 1%, the ratio between seven Plant Extracts is Gotu Kola P.E: polygonum cuspidate root extract: scutellariae,radix is mentioned
Take object: tea extract: glycyrrhiza glabra root extract: german chamomile flower extract: rosemary leaf extract=1:1:3:1:3:1:2
When, lotion has best bacteriostasis.This illustrates there is synergistic effect in toothpaste after lysozyme and plant extracts are compound
Fruit, and lead to active the case where the failing with plant extracts activity of failing of enzyme without generating to influence each other.
33~39 gained toothpaste of formula are placed 45 DEG C 3 months, using fragrance, pH, lotion character as inspection target, as a result 3
It is normal that it is formulated 33~39 gained toothpaste stability after a month, illustrates that lysozyme and plant extracts do not have lotion formulation stability
Have an impact.
39 gained toothpaste of formula are subjected to treatment gingivitis and bleeding gums clinical test in 3 months by a definite date, use tooth
Gum index (gingival index, GI), spy examine bleeding percentage (percentage of bleeding on probing,
BOP% the index for) evaluating gingivitis and bleeding gums, when off-test, test group gingivitis and bleeding gums it is each
Item Index for examination mitigates compared with control group, and difference has conspicuousness.Illustrate to be made using the compound of lysozyme and plant extracts
Lotion, have a more apparent improvement to the oral health of people, especially in terms for the treatment of gingivitis and bleeding gums.
Embodiment 3
According to embodiment 1 as a result, the compound of+seven Plant Extracts of lysozyme is applied in oral nursing liquid.
A kind of oral nursing liquid of the compositions of additives containing lysozyme and plant extracts, oral nursing liquid it is specific
Formula is shown in Table 6 formulas 40~46.
Preparation process is as follows:
The preparation of A group water-soluble component: moisturizer (polyethylene glycol, propylene glycol, glycerol), the surfactant agent (moon is added
Osmanthus acylsarcosine sodium, Cocoamidopropyl betaine), sweetener (saccharin sodium, Sucralose), water, stir 10~50min；
The preparation of B group oil-soluble constitents: solubilizer (Cremophor RH40, poloxamer188), essence, second is added
Alcohol stirs 10~50min；
Component C is lysozyme and plant extracts；B group is added in A group, stirs 10~50min；C group ingredient is added
It is adequately mixed in uniform AB mixed liquor, stirs 10~50min, obtain oral nursing liquid, can be sprayed for mouthwash or mouth.
According to the evaluation method of QB 2738-2012 daily chemical products antibacterial bacteriostatic effect, 40~46 gained oral cavities of measurement formula
Conditioning liquid to Streptococcus mutans, porphyromonas gingivalis, staphylococcus aureus, Escherichia coli inhibiting effect.Measurement result
As shown in table 6.
The composition and fungistatic effect of the formula of table 6 40~46
As can be seen from Table 6: (1) being made into using the lysozyme of raw material monomer, Gotu Kola P.E, radix scutellariae root extract
Oral nursing liquid, fungistatic effect are not satisfactory, wherein monomer lysozyme is slightly good, and to gram-positive bacteria golden yellow
Staphylococcus has preferable inhibitory or killing effect, slightly poor to Gram-negative bacteria inhibitory or killing effect, and the Gotu Kola P.E of monomer, radix scutellariae
Root extract totality fungistatic effect is low compared with monomer lysozyme；(2) it is made into using the compound of lysozyme compound botanical extract
Oral nursing liquid, fungistatic effect is compared with will get well using raw material monomer lysozyme and plant extracts, and fungistatic effect is from big
It is that+three Plant Extracts >+two kinds of plants of lysozyme of+seven Plant Extracts > lysozyme of lysozyme mention to minispread sequence
Take object, wherein when bacteriolyze enzyme dosage is 1%, the ratio between seven Plant Extracts is Gotu Kola P.E: polygoni cuspidati,radix is extracted
Object: radix scutellariae root extract: tea extract: glycyrrhiza glabra root extract: german chamomile flower extract: rosemary leaf extract=1:1:
When 3:1:3:1:2, oral nursing liquid has best bacteriostasis.This illustrates to be used in oral cavity after lysozyme and plant extracts are compound
There is cooperative synergism effect in conditioning liquid, and causes the activity failure of enzyme and plant extracts activity to be failed without generating to influence each other
The case where.
40~46 gained oral nursing liquids of formula are placed 45 DEG C 3 months, using fragrance, pH, water body character as inspection target,
As a result it is normal that it is formulated 40~46 gained oral nursing liquid stability after 3 months, illustrates lysozyme and plant extracts to oral cavity
Conditioning liquid formulation stability does not influence.
46 gained oral nursing liquids of formula are subjected to treatment gingivitis and bleeding gums clinical test in 3 months by a definite date,
Using gingival index (gingival index, GI), visits and examine bleeding percentage (percentage of bleeding on
Probing, BOP%) evaluation gingivitis and bleeding gums index, when off-test, test group gingivitis and gum
Every Index for examination of bleeding mitigates compared with control group, and difference has conspicuousness.Illustrate answering using lysozyme and plant extracts
Oral nursing liquid made of object is closed, has more apparent improvement to the oral health of people, especially in treatment gingivitis and tooth
In terms of oulorrhagia.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of shield range can also be made on the basis of above description and thinking for those of ordinary skill in the art
Other various forms of variations or variation, there is no necessity and possibility to exhaust all the enbodiments.It is all of the invention
Made any modifications, equivalent replacements, and improvements etc., should be included in the protection of the claims in the present invention within spirit and principle
Within the scope of.
Claims (10)
1. a kind of compositions of additives containing lysozyme and plant extracts, which is characterized in that the compositions of additives by
Lysozyme and plant extracts composition；The plant extracts is Gotu Kola P.E, polygonum cuspidate root extract, scutellariae,radix extraction
One of object, tea extract, glycyrrhiza glabra root extract, german chamomile flower extract, rosemary leaf extract are a variety of.
2. compositions of additives according to claim 1, which is characterized in that when plant extracts is a kind of, the bacteriolyze
The mass ratio of enzyme and plant extracts is (0.01~10): (0.1~10)；
When plant extracts is two kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10): (0.1~
10): (0.1~10)；
When plant extracts is three kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10): (0.1~
10): (0.1~10): (0.1~10)；
When plant extracts is four kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10): (0.1~
10): (0.1~10): (0.1~10): (0.1~10)；
When plant extracts is five kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10): (0.1~
10): (0.1~10): (0.1~10): (0.1~10): (0.1~10)；
When plant extracts is six kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10): (0.1~
10): (0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10)；
When plant extracts is seven kinds, the mass ratio of the lysozyme and each plant extracts is (0.01~10): (0.1~
10): (0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10): (0.1~10).
3. compositions of additives according to claim 2, which is characterized in that when plant extracts is Gotu Kola P.E, tiger
Cane root extract, radix scutellariae root extract, tea extract, glycyrrhiza glabra root extract, german chamomile flower extract, rosemary leave are extracted
When any in object, the mass ratio of the lysozyme and plant extracts is 1:2.
4. compositions of additives according to claim 2, which is characterized in that when plant extracts is Gotu Kola P.E and tiger
When cane root extract, the mass ratio of the lysozyme and each plant extracts is 1:1:1.
5. compositions of additives according to claim 2, which is characterized in that when plant extracts is Gotu Kola P.E, tiger
When cane root extract and radix scutellariae root extract, the mass ratio of the lysozyme and each plant extracts is 1:0.66:0.66:
0.66。
6. compositions of additives according to claim 2, which is characterized in that when plant extracts is Gotu Kola P.E, tiger
When cane root extract, radix scutellariae root extract and tea extract, the mass ratio of the lysozyme and each plant extracts is 1:
0.5:0.5:0.5:0.5.
7. compositions of additives according to claim 2, which is characterized in that when plant extracts is Gotu Kola P.E, tiger
When cane root extract, radix scutellariae root extract, tea extract and glycyrrhiza glabra root extract, the lysozyme and each plant extract
The mass ratio of object is 1:0.4:0.4:0.4:0.4:0.4.
8. compositions of additives according to claim 2, which is characterized in that when plant extracts is Gotu Kola P.E, tiger
When cane root extract, radix scutellariae root extract, tea extract, glycyrrhiza glabra root extract and german chamomile flower extract, the bacteriolyze
The mass ratio of enzyme and each plant extracts is 1:0.33:0.33:0.33:0.33:0.33:0.33.
9. compositions of additives according to claim 2, which is characterized in that when plant extracts is Gotu Kola P.E, tiger
Cane root extract, radix scutellariae root extract, tea extract, glycyrrhiza glabra root extract, german chamomile flower extract and rosemary leave mention
When taking object, the mass ratio of the lysozyme and each plant extracts is 1:0.17:0.17:0.5:0.17:0.5:0.17:0.33.
10. any one of claim 1 to 9 compositions of additives is in the oral care implement that preparation has bacteriostasis efficacy
Using.
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| CN111701014A (en) * | 2020-08-12 | 2020-09-25 | 好易康生物科技(广州)有限公司 | Toothpaste for children and preparation method thereof |
| CN112315842A (en) * | 2020-10-27 | 2021-02-05 | 杭州宠趣生物科技有限公司 | Dog and cat oral care spray containing lysozyme and preparation method thereof |
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