CN105534827A - Toothpaste containing lysozyme - Google Patents
Toothpaste containing lysozyme Download PDFInfo
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- CN105534827A CN105534827A CN201610085933.4A CN201610085933A CN105534827A CN 105534827 A CN105534827 A CN 105534827A CN 201610085933 A CN201610085933 A CN 201610085933A CN 105534827 A CN105534827 A CN 105534827A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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Abstract
The invention relates to toothpaste containing lysozyme. The weight ratio of the active ingredients, namely lysozyme to a gynura procumbens extract in the toothpaste is (1:10)-(10:1). In comparison with common Chinese herbal medicine toothpaste, the lysozyme toothpaste provided by the invention is simple in preparation method and reasonable in compatibility, and the two active ingredients cal generate a synergistic action while giving play to respective effects, thereby significantly preventing oral cavity gingivitis and dental plaque. The lysozyme and gynura procumbens have natural sources, are safe and nontoxic, and have no side effects; and as the toothpaste does not contain a western medicine ingredient, the toothpaste has no drug tolerance.
Description
Technical field
The present invention relates to oral hygiene field, be specifically related to a kind of toothpaste.
Background technology
Medically will around and cover periodontal soft tissue and be called gingiva, the active chronic inflammation betiding gingiva tissue is called gingivitis.Show as gingival hemorrhage, red and swollen, distending pain, continues development and invades sclerous tissues, produce periodontitis, comprises the performance at gingiva of the inflammation of gingiva tissue and general disease.Dental plaque is the adhered to one another of substrate parcel or adheres between facing, tooth or dummy surface soft and the bacillary colony of non-mineralising, for can not being washed away by water or gargling a kind of Bacterial biofilms fallen.When dental plaque amount is less, naked eyes are difficult to observe, and usual plaque indicator agents can show well.
Toothpaste is auxiliary a kind of preparation of brushing teeth, and can strengthen the frictional force of brushing teeth, and helps to remove food scraps, soft dirt and dental plaque, contributes to eliminating or alleviating halitosis.By mechanical methods, thoroughly removal dental plaque or control gingivitis are difficult to accomplish for most people.Therefore, researcher proposes in toothpaste, add certain antibacterial.At present, be mainly Western medicine for the antibacterial prevented and treated in the functional toothpaste of gingivitis and dental plaque on the market, life-time service easily produces drug resistance.Biological enzyme toothpaste is a kind of toothpaste containing bioenzyme activity composition and the composition such as enzyme stabilizers, activator, and it can further bactericidal, promotes that oral cavity is cleaned further, thus takes care of one's teeth, strengthen the defense system in oral cavity self.
Lysozyme (lysozyme), also known as muramidase (muramidase) or N-acetylmuramide lycanohydrlase (N-acetylmuramideglycanohydrlase), is a kind of alkaline enzyme that can be hydrolyzed mucopolysaccharide in pathogenic bacterium.Mainly through the β-Isosorbide-5-Nitrae glycosidic bond between the-acetylmuramic acid in destruction cell wall and NAG, make the insoluble mucopolysaccharide of cell wall resolve into solubility glycopeptide, cause cell wall rupture content overflow and make bacterolysis.Lysozyme also directly can be combined with electronegative virus protein, forms double salt, make virally inactivated with DNA, RNA, apoprotein.Therefore, this enzyme has the effects such as antibacterial, antiinflammatory, antiviral.Lysozyme (also claiming lyase) is a molecular weight is the enzyme of 14.4kDa, it can by 1 between-acetylmuramic acid and 2-Acetamido-2-deoxy-D-glucose residue and in chitodextrin between N-acetyl-glucosamine residue in catalysis Peptidoglycan, the hydrolysis of 4-β chain, and destroy the cell wall of antibacterial.In human body, lysozyme at emiocytosis liquid, as extensively existed in saliva, tear and some other body fluid; Also be present in the cytoplasmic granule body in mitochondrion.In addition, a large amount of lysozyme is also had in Ovum Gallus domesticus album.Lysozyme, because of the alkali hydrolysising protease acting on the cell wall of object microorganism that can be single-minded, is applied to the industries such as food, feedstuff, medicine widely.
Lysozyme (lysozyme) is by the simple alkaline globulin of 18 kinds of 129 Amino acid profiles, and molecular weight is 14.4KDa.Chemical property is highly stable.When pH value is acute variation in 1.2 ~ 11.3 scopes, its structure is almost constant.In sour environment, lysozyme is very strong to the stability of heat; When pH value is 4 ~ 7,100 DEG C of process 1min still can keep original enzyme activity; Ability 100 DEG C of heat treated 45min when pH value is 3; In dry conditions, lysozyme can be deposited in room temperature for a long time, and its sterling is white or micro-yellow.Yellow crystalline solid or amorphous powder, odorless, taste is sweet.Soluble in water, suffer alkali destructible, be insoluble to acetone and ether.As medical supplies, lysozyme has good biocompatibility, to organizing the advantages such as non-stimulated, avirulence.
Lysozyme is extensively present in poultry, the Ovum Gallus domesticus album of birds and mammiferous tear, saliva, blood plasma, urine, milk, leukocyte and other body fluid and tissue (as liver, kidney) cell; From Fructus Hordei Vulgaris, Fructus Fici, Fructus Chaenomelis and the plant such as Brassica oleracea L.var.capitata L., Radix Raphani, also can isolate lysozyme, be wherein the highest (about containing 0.3%) with content in Ovum Gallus domesticus album.And human milk, tear, lysozyme activity in saliva is far above (being about 3 times) in Ovum Gallus domesticus album or the activity of the lysozyme in other sources.Lysozyme in Ovum Gallus domesticus album is the Typical Representative of animal lysozyme, is object of study important in lysozyme group, is also to understand the most clearly one of lysozyme at present.In mammiferous milk, the lysozyme content in lacto is 3000 times of Lac Bovis seu Bubali.By sources different, lysozyme can be divided into egg white lysozyme, animal lysozyme, plant lysozyme, the lysozyme of microorganism generation and the lysozyme of bacteriophage generation; Different by function cells wall, lysozyme can be divided into bacteria cell wall lysozyme and fungal cell wall lysozyme.
Lysozyme can the cell wall of single-minded cracking object antibacterial.Its main attack Peptidoglycan, hydrolysis connects the β-Isosorbide-5-Nitrae glycosidic bond of-acetylmuramic acid and N-acetyl-glucosamine the 4th carbon atom.Whole process is, first lysozyme is attached on Peptidoglycan molecule by " ditch " between two domain; Its substrate forms the conformation of transition state in enzyme subsequently.According to Phillips mechanism, lysozyme is combined with glucohexaose.Then the sugared distortion of the 4th on glucohexaose is half-chair conformation by lysozyme.In this twisted state (energy is higher), glycosidic bond is easy to just rupture.The side chain being positioned at the glutamic acid (Glu35) of antalzyme protein sequence 35 and the aspartic acid (Asp52) of 52 is very crucial to the activity of lysozyme.Glu35, as the proton donor of glycosidic bond, shears the C-O key of substrate; And Asp52 participates in generating glycosyl enzyme intermediate as nucleopilic reagent.Subsequently, glycosyl enzyme intermediate and hydrone react, and hydrolysis generates product, and enzyme remains unchanged.
The extracting method of lysozyme comprises following several: 1, direct crystallization method: this method is simple to operate, and cost is low, but can not in order to be separated the lysozyme of trace.2, ion exchange chromatography: this method have easy, efficient, cost is low, and can the advantage circle of automatization's continued operation.3, affinity chromatograph: the multiple of affinity chromatography separation and purification is higher, quality better, and can be widely used in the lysozyme in various source, for the lysozyme of concentrated and refining trace.But because the making of affinity adsorbent is more complicated, limit its extensive utilization industrially.4, ultrafiltration is combined with affinity chromatograph: ultrafiltration is combined with affinity chromatograph and extracts lysozyme, is the new method developed in recent years.First remove most of foreign protein with ultrafiltration, retain lysozyme, now lysozyme is also comparatively thick, then obtains purified product with affinity chromatography., there is affinity film chromatography technology in 5, membrane chromatography technology: late 1980s.This technology has the feature of membrance separation and Human serum protein concurrently.Compared with traditional membrance separation, affinity chromatography; membrane chromatography technology not only has that purification is high, pressure drop is little, disengaging time is short, biomacromolecule degeneration probability in separation process is little; and allow the feature such as charging rate faster, and more easily accomplish scale production than post affinity chromatography.6, ultrafiltration: compared with traditional bio-chemistry separation technology, the main advantage of hyperfiltration technique is the high yield output of product.Although but hyperfiltration technique is widely used in reverse osmosis and the process such as concentrated, as a kind of protein stripping technique that potential in biological industry field is very large, ultrafiltration is fully utilized not yet.7, reverse micelle extracts: reverse micelle extraction is a kind of new extracting process that developed recently gets up, it is the method utilizing in organic solvent the reverse micelle adding the formation of a small amount of surfactant to extract, the bioactive substances such as the enzyme that can not with an organic solvent extract for some and reactive protein have opened up a kind of new isolation technics, expand the scope of application of organic solvent extraction.8, bioseparation technology: the bioseparation technology (immobilized metal affinity chromatography, affinity precipitation, affinity filtration method) based on affinity is also one of lysozyme extracting method.
Lysozyme has multiple uniqueness and important pharmacological action.
The first, anti-inflammation.The cell wall of antibacterial is made up of murein, the polymer that murein is alternately made up of 2-Acetamido-2-deoxy-D-glucose and-acetylmuramic acid, can connecting peptides on cell wall residue, is called Peptidoglycan.Polysaccharide exists with linear form, can be interconnected between polysaccharide chain located adjacent one another by peptide chain part, thus forms three dimensional structure.Lysozyme acts on the glycosidic bond between the-acetylmuramic acid of Peptidoglycan molecule and N-acetyl glucosamine ammonia in specific manner, and result makes bacteria cell wall become loose, loses the protective effect to cell, and last cytolysis is dead; Lysozyme directly can kill gram positive bacteria, in the presence of S-IgA complement, can also kill gram-negative bacteria: as colon bacillus.Lysozyme also can be combined with the various acidic materials bringing out inflammation, reduces bacterial endotoxin release, thus alleviates endotoxemia generation, slow down inflammation generating process, and certain potentiation is have to antibiosis, improve the mucopolysaccharide metabolism of periplast, play the effect of anti-inflammation, repair tissue.Prove through test, with a kind of cellulose copolymer by human lysozyme and proteolytic enzyme coupling, lysozyme antibiotic ability can be significantly improved, accelerating wound healing.
The second, antiviral.Lysozyme under neutral fluid environment with a large amount of positive charge, can with the virus protein direct effect with negative charge, combine with DNA, RNA, desorption base albumen formation double salt, makes virally inactivated.T suppression cell Denaturation is had after adding lysozyme in by the Hela cell culture fluid of herpesvirus infection.Lysozyme also can suppress adenovirus to grow, and can be used for the viral illness treatment such as herpes zoster, parotitis, chicken chickenpox, hepatitis and influenza.
3rd, enhancing immunity.Lysozyme participates in panimmunity reaction in body, in body normal defense and nonspecific immunity, have important function.It can improve and strengthen macrophage phagocytic digestive function, reduces the leukopenia that cytostatics causes, in conjunction with bacteria lipopolysaccharide, alleviates endotoxin effect, to reach the object of enhancing body resistance.Lysozyme itself has t cell epitope, and 2 types (Th2) t helper cell can be induced to react.Oral lysozyme energy inducing mouse produces general Th1 and Th2 immunoreation, strengthens immunologic function, and therefore, oral lysozyme has the function controlling upper respiratory tract infection and treatment dysentery.In addition, lysozyme also has the hematoblastic function of activation, can improve tissue local disturbance of blood circulation, decomposes pus, strengthens local defense function, thus embodies the repair that it stops blooding, subsides a swelling and accelerate body injury tissue.It also can be used as a kind of host's resistance factor, shields to tissue local.
Lysozyme is anticorrosion in food fresh keeping, oral cavity disinfecting, Water Disinfection, air purification and sterilizing and household electrical appliance sterilizing etc. are widely used in field.Research finds that lysozyme can be used for preservative of chilled meat, and this antistaling agent adopts safe, nontoxic, crude scientifically to coordinate composition completely, can extend the shelf-life 1-4 of Chilled Meats doubly.This antistaling agent can tolerate the temperature of less than 95 DEG C, and retention properties is stablized.Lysozyme also can be used for the anticorrosion of milk product, adds a certain amount of lysozyme and can prevent the foaming of middle and late stage cheese, spoiled in cheese processing process; The quality of cheese base fluid in cheese ageing process can also be kept, the more important thing is that it can play long-term bactericidal action, effectively control the fermentation of butyric acid.In addition, lysozyme also can be used for the aspects such as the sterilizing of anticorrosion, the aquatic products of the anti-bacterial refreshing of food flexible packing material, wine and beverage.
Antibacterial in air is adsorbed on dirt particle, common pathogenic bacterium have Mycobacterium tuberculosis, diphtheria corynebacterium, bordetella pertussis, staphylococcus aureus, micrococcus scarlatinae, Neisseria meningitidis, legionella etc., can cause respiratory infectious disease or wound infection.Consider safety factors, usually adopt the filters such as glass fibre HEPA to carry out filtration, purification to air degerming.But microorganism is easily bred, dispersed on HEPA, form secondary pollution.The domestic people of having has studied successfully a kind of lysozyme PPHEPA filter, and it by immobilized for lysozyme, can catch killing microorganisms at fibrous inside, effectively prevent secondary pollution on the whole filter paper fibre of HEPA.
Health type household electrical appliance are trend and the trend of current household electric appliances.Be applied to the many employing tradition of the sanitary disinfecting technology of white goods industry and physical sterilization and chemical disinfection at present, have potential safety hazard, secondary pollution problems.Sterilization lysozyme being applied to white goods industry can solve the problem of this respect well.Current lysozyme sterilization technology all has tentative application on air cleaner, central air-conditioning, purifier.
Antibacterial is the main cause that oral disease occurs, and dental caries, pulpitis, periapical periodontitis and part mucosa are sick all inseparable with the infection of oral cavity bacterium.Lysozyme is antimicrobial component important in people's saliva, participates in host to the nonimmune defence of antibacterial, regulates the homeostasis of oral environment, has certain relation with the generation development of opposing oral disease.Lysozyme can suppress the growth of dental decayed tooth, suppresses thalline glycolysis and produces acid, so most scholar thinks that lysozyme has certain effect to caries prevention.In addition, lysozyme also can treat oral ulcer, also also can be used as cleaning agent for mouth cavity simultaneously, take care of one's teeth and oral health.In oral cavity, apply lysozyme can repair tissue, is conducive to the healing of oral ulcer.Staphylococcus lysozyme and synergist, stabilizing agent also can be made into mouth cleaning solution, to several pathogenic bacterium causing oral disease, as staphylococcus, Hemolytic streptococcus, Candida albicans, anaerobe etc. have inhibitory or killing effect.Through detecting, in 5min, sterilizing rate reaches 99.9%.The safety evaluatios such as acute toxicity test, cumulative toxicity test, micronucleus test, mucosa irritation test all meet the requirements.Extracorporeal disinfecting test shows, lysozyme compound formulation effectively can suppress and kill pars oralis pharyngis pathogenic bacterium, can effectively treat the oral disease such as acute/chronic pharyngitis, tonsillitis.Because lysozyme has good curative effect to the treatment of oral disease and prevention, current domestic existing lysozyme buccal tablet listing, lysozyme has very wide prospect in oral disease.
Gynura procumbens (Lour.) Merr. (Gynuraprocumbens (Lour.) Merr) is Compositae Radix Notoginseng platymiscium, herbaceos perennial, another name Herba Gynurae procumbentis, tree Radix Notoginseng, stone Radix Notoginseng etc.Climb up by holding on to draft, have foul smell, stem is crawled, filbert or purple, has bar rib, has pubescence, have branch without when hair or children; Leaf tool handle; Blade ovum shape Long Circle or ellipse, long 3 ~ 8 centimetres, wide 1.5 ~ 3.5 centimetres; Top life or the raw corymb of axil, each corymb has 3 ~ 5 head inflorescences; The narrow mitriform of involucre or funnel-form, base portion has 5 ~ 6 linear squamellas, and each head inflorescence has little Hua 20 ~ 30, and pattern is orange; The long 12-15 millimeter of corolla, pipe portion is thin, long 8-10 millimeter, and top expands, sliver ovum shape lanceolar, top point; Achene is cylindrical, long 4 ~ 6 millimeters, Chestnut.The place of production is distributed in the ground such as Guangdong, Hainan, Guizhou, Yunnan, is often born in by woodland, small stream, on the sand in hillside fields, climbs up by holding on on shrub or arbor.Also there is distribution in Vietnam, Thailand, Indonesia and Africa.
Gynura procumbens (Lour.) Merr. young stem and leaf is nutritious, its protein, aminoacid, total sugar, fat content are respectively 1.24%, 0.87%, 1.5%, 0.79%, dietary fiber content is 2.40 grams/100 grams, Vitamin C content is 11.2 milligrams/100 grams, be rich in organic calcium, the pollution-free food that middle-aged and elderly people is replenished the calcium, also containing phenolic acid compounds such as flavonoid, triterpene aglycon class, phytosterin compound and vanillin, syringic aldehyde, P-hydroxybenzoic acid, p-hydroxycinnamic acid, protocatechuic acid.
Gynura procumbens (Lour.) Merr. is a kind of unique plant of dietotherapeutic, and it is mainly edible is partly young stem and leaf.The Herba basellae rubrae that it and present people eat belongs to together, and food flavor is soft and smooth, and delicate fragrance is good to eat, can fry, cold and dressed with sauce, float soup, cooks dumpling, the filling of steamed bun has much fragrance after the chopping of its stem and leaf, is used for brew meal, and its taste is especially good.Its leaf is also raw-eaten, or gets fresh leaf boiled water and brew and work as tea-drinking, as being used for soaked drinking after being dried by blade, does not then have some local flavor especially.
Gynura procumbens (Lour.) Merr. whole body is all precious, and rhizome is the excellent raw material of Chinese patent medicine, its slightly sweet flavor, and property is put down, can dredge the meridian passage, inflammation-diminishing and cough-controlling, scattered silt detumescence, blood activating and promoting tissue regeneration.Cook edible or medicinal, there is blood fat reducing, blood pressure lowering, hypoglycemic activity, replenish the calcium in addition, influenza and hepatitis B virus, anti-flu, improve the effects such as body immunity, external all has good curative effect to herpes zoster, various dermatitis, scald, burn and nameless gall, can also stop blooding, pain relieving, antipruritic, skin protection moisturizing and defying age.
At present, lysozyme and Gynura procumbens (Lour.) Merr. are combined and be used for preventing and treating odontopathy, there is not been reported in the application of particularly gingivitis and dental plaque.
Summary of the invention
Technical problem to be solved by this invention is to provide one can effectively prevent and treat gingivitis and dental plaque, has no side effect and drug resistance, and active component is originated natural functional toothpaste.
The present invention's adopted technical scheme that solves the problem is, provide a kind of ointment containing lysozyme, wherein the weight ratio of active component lysozyme and Gynura procumbens (Lour.) Merr. extract is (1:10) ~ (10:1).
Preferably, in described toothpaste, the weight ratio of lysozyme and Gynura procumbens (Lour.) Merr. extract is (1:5) ~ (5:1).
Preferred, the weight ratio of lysozyme and Gynura procumbens (Lour.) Merr. extract is 1:4.
Preferably, described Gynura procumbens (Lour.) Merr. extract is the ethanol extraction of Gynura procumbens (Lour.) Merr. stem.
Preferably, described toothpaste comprises one or more in abrasivus, wetting agent, binding agent, sweeting agent, water further.
Preferred, described abrasivus be selected from calcium hydrogen phosphate, silicon dioxide etc. one or more; Described wetting agent be selected from sorbitol, propylene glycol etc. one or more; Described binding agent be selected from carrageenan, Konjac glucomannan, sodium carboxymethyl cellulose etc. one or more; Described sweeting agent is saccharin sodium.
Preferably, in the toothpaste of described 100 weight portions, the lysozyme containing 0.5-5 % by weight and Gynura procumbens (Lour.) Merr. extract.
The present invention also provides the application of a kind of above-mentioned lysozyme toothpaste in the toothpaste preparing control gingivitis or dental plaque.
The present invention has actively useful effect:
Surprisingly, through repeated tests, the present invention surprisingly finds lysozyme and Chinese herbal medicine Gynura procumbens (Lour.) Merr. extract combination to use the control for gingivitis and dental plaque to have the effect of Synergistic, coordinates other active component results of use better.
Compared with common Chinese herbal toothpaste, lysozyme toothpaste preparation method of the present invention is simple, and compatibility is reasonable, and two kinds of active component, while the respective effect of performance, can produce synergistic function again, significantly control dental gingivitis and dental plaque.Lysozyme and Gynura procumbens (Lour.) Merr. are originated natural, and safety non-toxic, has no side effect; Due in toothpaste without western medicine composition, therefore toothpaste has no drug resistance.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described, but embodiments of the present invention are not limited thereto.The experimental technique used in following embodiment if no special instructions, is conventional method.
The extraction of embodiment 1, Gynura procumbens (Lour.) Merr.
After Gynura procumbens (Lour.) Merr. stem powder is crossed 40 mesh sieves, with petroleum ether decolouring defat 3h, use 90% ethanol of 30 times of weight in 80 DEG C of heating and refluxing extraction 3 times after being dried to constant weight again, each 2 hours, merge extractive liquid, after concentrate drying and get final product.
The preparation of embodiment 2, lysozyme/Gynura procumbens (Lour.) Merr. extract toothpaste
Get the Gynura procumbens (Lour.) Merr. extract that 5 weight portions are obtained by embodiment 1, the silicon dioxide of 50 weight portions, the sorbitol of 20 weight portions, the carrageenan of 1 weight portion, the saccharin sodium of 0.2 weight portion and the water mixing of surplus, make the toothpaste A1 containing Gynura procumbens (Lour.) Merr. extract of 100 weight portions.
Get the lysozyme of 5 weight portions, the silicon dioxide of 50 weight portions, the sorbitol of 20 weight portions, the carrageenan of 1 weight portion, the saccharin sodium of 0.2 weight portion and the water mixing of surplus, make the toothpaste A2 containing lysozyme of 100 weight portions.
Get the lysozyme of 4.167 weight portions, the Gynura procumbens (Lour.) Merr. extract obtained by embodiment 1 of 0.833 weight portion, the silicon dioxide of 50 weight portions, the sorbitol of 20 weight portions, the carrageenan of 1 weight portion, the saccharin sodium of 0.2 weight portion and the water mixing of surplus, make the toothpaste A3 containing lysozyme and Gynura procumbens (Lour.) Merr. extract (weight ratio is 5:1) of 100 weight portions.
Get the lysozyme of 2.5 weight portions, the Gynura procumbens (Lour.) Merr. extract obtained by embodiment 1 of 2.5 weight portions, the silicon dioxide of 50 weight portions, the sorbitol of 20 weight portions, the carrageenan of 1 weight portion, the saccharin sodium of 0.2 weight portion and the water mixing of surplus, make the toothpaste A4 containing lysozyme and Gynura procumbens (Lour.) Merr. extract (weight ratio is 1:1) of 100 weight portions.
Get the lysozyme of 1 weight portion, the Gynura procumbens (Lour.) Merr. extract obtained by embodiment 1 of 4 weight portions, the silicon dioxide of 50 weight portions, the sorbitol of 20 weight portions, the carrageenan of 1 weight portion, the saccharin sodium of 0.2 weight portion and the water mixing of surplus, make the toothpaste A5 containing lysozyme and Gynura procumbens (Lour.) Merr. extract (weight ratio is 1:4) of 100 weight portions.
Get the lysozyme of 0.833 weight portion, the Gynura procumbens (Lour.) Merr. extract obtained by embodiment 1 of 4.167 weight portions, the silicon dioxide of 50 weight portions, the sorbitol of 20 weight portions, the carrageenan of 1 weight portion, the saccharin sodium of 0.2 weight portion and the water mixing of surplus, make the toothpaste A6 containing lysozyme and Gynura procumbens (Lour.) Merr. extract (weight ratio is 1:5) of 100 weight portions.
The application in gingivitis and dental plaque prevented and treated by embodiment 3, the toothpaste comprising lysozyme/Gynura procumbens (Lour.) Merr. extract
3.1 object of study
The double blinding that adult men and women's volunteer of health is carried out, layering, parallel clinical research.First made clear experiment purpose, research details by researcher to experimental subject, participate in the responsibility studied.Be ready that the experimenter participated in fills in voluntary postscript, by researcher, it checked, judge according to including in exclusion standard, screen tested crowd.Preliminary screening process comprises oral soft tissue and sclerous tissues checks, gingivitis and bacterial plaque inspection are to determine whether being selected in of experimental subject.Finally 210 object of study of selected improvement Quigley-Hein plaque index >=1.5 and Loe-Silness index of gingivitis >=1.0 have participated in research, and be divided into 7 balance groups according to plaque index and gingival index, often organize 30 people, men and women half and half, and experimental subject the range of age is 20 ~ 40 years old.
3.2 inspection method
The Turesky plaque index of Quigley-Hein improvement is adopted to evaluate supragingival plaque.To supragingival plaque dyeing and according to following standard recording:
0=is without bacterial plaque;
The bacterial plaque that 1=neck portion marginal existence is dispersed in;
The thin bacterial plaque band (reaching lmm wide) of the visible continuous print in 2=neck edge;
3=neck portion bacterial plaque band is greater than lmm, but is less than 1/3 of facing;
4=bacterial plaque covers 1/3 ~ 2/3 of facing;
5=bacterial plaque covers facing more than 2/3.
Tooth detection buccal surface and lingual surface are 10 for the maximum numeration of each tooth.Except third molar, artificial tooth and the tooth having cervical region dummy, remaining tooth is all at the row of numeration.The mean of each experimenter's plaque index counts sum (each tooth 2 record) by individual's whole tooth detection gained and obtains divided by total measurement number (teeth number is multiplied by 2).
Gingivitis adopts improvement Loe-Silness index of gingivitis to measure, and measures the pre-test gingiva situation of plaque index in dyeing.Gingivitis method of counting and standard as follows:
0=is without gingivitis;
Gingivitis that 1=is slight, gingiva color and luster and quality slightly change, but spy is examined without hemorrhage;
2=moderate inflammation, gingiva light.Red, swollen, hypertrophy, spy are examined hemorrhage;
3=hyperphlogosis, gingiva obviously rubescent, hypertrophy, have hematostaxis.
As the inspection of plaque index, each tooth inspection record buccal surface and lingual surface, a maximum numeration of tooth is 6.Except third molar, artificial tooth and the tooth having cervical region dummy, remaining tooth includes the row in numeration.The mean of Loe-Silness index of gingivitis refers to that the summation (each tooth 2 face) of all tooth counter value is divided by total measuring surface number (tooth of measurement takes advantage of 2) institute value.
3.3 experimental techniques and result
After completing baseline inspection (soft, sclerous tissues, bacterial plaque, gingivitis), all experiments object accepts clean one's teeth and be divided into 7 balance groups to participate in this research according to bacterial plaque and gingivitis situation.Random is assigned as 6 experimental grouies (A1-A6) and 1 matched group (without any active component, the adjuvant in all the other toothpaste is identical with A1-A6, and excess water complements to 100 weight portions) that use embodiment 2 toothpaste.
Experimenter adopts unified soft toothbrush and uses toothpaste by regulation, and require to brush teeth for 1st 2 times (early, evening), each 1min.To require in experimentation without any other dental care product as other toothpaste or toothbrush, collutory, dental floss etc.Before experiment, good balance grouping is carried out, without statistics difference between each group from number, age, sex, bacterial plaque and index of gingivitis to experimental subject.When using toothpaste 6 months, assess experimenter's dental plaque and gingivitis situation according to the method described above, the statistical result after 6 months after clinical examination is in table 1.
The plaque index of each experimental group experimenter and index of gingivitis after table 16 month
| Plaque index | Index of gingivitis | |
| Matched group | 2.79 | 1.87 |
| A1 experimental group (Gynura procumbens (Lour.) Merr. extract) | 2.41 | 1.55 |
| A2 experimental group (lysozyme) | 2.50 | 1.68 |
| A3 experimental group (lysozyme of 5:1 and Gynura procumbens (Lour.) Merr. extract) | 2.05 | 1.03 |
| A4 experimental group (lysozyme of 1:1 and Gynura procumbens (Lour.) Merr. extract) | 1.72 | 1.05 |
| A5 experimental group (lysozyme of 1:4 and Gynura procumbens (Lour.) Merr. extract) | 1.51 | 0.71 |
| A6 experimental group (lysozyme of 1:5 and Gynura procumbens (Lour.) Merr. extract) | 1.98 | 0.99 |
As can be seen from the contrast experiment of embodiment 3, lysozyme toothpaste of the present invention can effectively prevent and treat gingivitis and dental plaque, particularly lysozyme and combinationally using of Gynura procumbens (Lour.) Merr. extract creates synergy, decreases the use amount of active component.When active component total amount remains unchanged, use the toothpaste (A3-A6) of lysozyme of the present invention and Gynura procumbens (Lour.) Merr. extract to be comparatively used alone the index of gingivitis of toothpaste (A1 and the A2) experimenter of lysozyme or Gynura procumbens (Lour.) Merr. extract and plaque index and all have reduction in various degree, the Be very effective of proved invention toothpaste in control gingivitis and dental plaque, wherein lysozyme is the most outstanding than the toothpaste effect being 1:4 with Gynura procumbens (Lour.) Merr. extract weight, plaque index and index of gingivitis are only 1.51 and 0.71 respectively, be significantly higher than matched group, create the excellent effect being difficult to expect.
Claims (8)
1. the toothpaste containing lysozyme, it is characterized in that, wherein the weight ratio of active component lysozyme and Gynura procumbens (Lour.) Merr. extract is (1:10) ~ (10:1).
2. toothpaste according to claim 1, is characterized in that, the weight ratio of lysozyme and Gynura procumbens (Lour.) Merr. extract is (1:5) ~ (5:1).
3. toothpaste according to claim 2, is characterized in that, the weight ratio of lysozyme and Gynura procumbens (Lour.) Merr. extract is 1:4.
4. the toothpaste according to any one of claim 1-3, is characterized in that, described Gynura procumbens (Lour.) Merr. extract is the ethanol extraction of Gynura procumbens (Lour.) Merr. stem.
5. toothpaste according to claim 1, is characterized in that, toothpaste comprise in abrasivus, wetting agent, binding agent, sweeting agent, water further one or more.
6. toothpaste according to claim 5, is characterized in that, described abrasivus be selected from calcium hydrogen phosphate, silicon dioxide etc. one or more; Described wetting agent be selected from sorbitol, propylene glycol etc. one or more; Described binding agent be selected from carrageenan, Konjac glucomannan, sodium carboxymethyl cellulose etc. one or more; Described sweeting agent is saccharin sodium.
7. want the toothpaste described in 1 according to right, it is characterized in that, in the toothpaste of described 100 weight portions, the lysozyme containing 0.5-5 % by weight and Gynura procumbens (Lour.) Merr. extract.
8. the application of the toothpaste containing lysozyme described in any one of claim 1-7 in the toothpaste preparing control gingivitis or dental plaque.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105943408A (en) * | 2016-06-07 | 2016-09-21 | 上海舒雅心日用品有限公司 | Fluoride-free anti-infection toothpaste with natural sterilization effect and preparation method thereof |
| EP3192495A1 (en) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Oral care compositions |
| CN107714611A (en) * | 2017-11-13 | 2018-02-23 | 常州麒通国际贸易有限公司 | A kind of edible toothpaste for children |
| KR20180098754A (en) * | 2017-02-27 | 2018-09-05 | 김동환 | Organic acid composition to be added to antimicrobial toothpaste and method for producing the same |
| CN110237241A (en) * | 2019-07-05 | 2019-09-17 | 广州康云生物科技有限公司 | A kind of compositions of additives containing lysozyme and plant extracts |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3192495A1 (en) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Oral care compositions |
| CN105943408A (en) * | 2016-06-07 | 2016-09-21 | 上海舒雅心日用品有限公司 | Fluoride-free anti-infection toothpaste with natural sterilization effect and preparation method thereof |
| KR20180098754A (en) * | 2017-02-27 | 2018-09-05 | 김동환 | Organic acid composition to be added to antimicrobial toothpaste and method for producing the same |
| KR101899237B1 (en) | 2017-02-27 | 2018-09-14 | 김동환 | Organic acid composition to be added to antimicrobial toothpaste and method for producing the same |
| CN107714611A (en) * | 2017-11-13 | 2018-02-23 | 常州麒通国际贸易有限公司 | A kind of edible toothpaste for children |
| CN110237241A (en) * | 2019-07-05 | 2019-09-17 | 广州康云生物科技有限公司 | A kind of compositions of additives containing lysozyme and plant extracts |
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|---|---|
| CN105534827B (en) | 2018-02-23 |
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Denomination of invention: A toothpaste containing lysozyme Effective date of registration: 20210630 Granted publication date: 20180223 Pledgee: Agricultural Bank of China Limited by Share Ltd. Pujiang County branch Pledgor: ZHEJIANG AEGIS BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. Registration number: Y2021330000759 |
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