CN105506135B - 一种含有pfb-2引物对的猪粪便污染检测试剂盒及其制备与应用 - Google Patents
一种含有pfb-2引物对的猪粪便污染检测试剂盒及其制备与应用 Download PDFInfo
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- CN105506135B CN105506135B CN201610037363.1A CN201610037363A CN105506135B CN 105506135 B CN105506135 B CN 105506135B CN 201610037363 A CN201610037363 A CN 201610037363A CN 105506135 B CN105506135 B CN 105506135B
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- swine excrement
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- pollution detection
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- excrement pollution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
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- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
正向引物(PFB-2F) | CCTTTGTCCTCAGTGAAGA | SEQ ID NO.1 |
反向引物(PFB-2R) | TCGGAATTCCGCTTGCCTCTGATCC | SEQ ID NO.2 |
正向引物(FUP-F) | CTAACTACGTGCCAGCAGCC | SEQ ID NO.3 |
反向引物(FUP-R) | GCCTTCGCCACTGGTGTTCC | SEQ ID NO.4 |
正向引物(PFB-2F)(10uM/L) | 0.5uL |
反向引物(PFB-2R)(10uM/L) | 0.5uL |
2×Power Taq PCR Master Mix | 7.5uL |
样品基因组DNA | 1uL |
无菌水补足至 | 15 uL |
正向引物(FUP-F)(10uM/L) | 0.5uL |
反向引物(FUP-R)(10uM/L) | 0.5uL |
2×Power Taq PCR Master Mix | 7.5uL |
样品基因组DNA | 1uL |
无菌水补足至 | 15 uL |
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610037363.1A CN105506135B (zh) | 2016-01-20 | 2016-01-20 | 一种含有pfb-2引物对的猪粪便污染检测试剂盒及其制备与应用 |
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CN201610037363.1A CN105506135B (zh) | 2016-01-20 | 2016-01-20 | 一种含有pfb-2引物对的猪粪便污染检测试剂盒及其制备与应用 |
Publications (2)
Publication Number | Publication Date |
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CN105506135A CN105506135A (zh) | 2016-04-20 |
CN105506135B true CN105506135B (zh) | 2019-01-15 |
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CN201610037363.1A Expired - Fee Related CN105506135B (zh) | 2016-01-20 | 2016-01-20 | 一种含有pfb-2引物对的猪粪便污染检测试剂盒及其制备与应用 |
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CN (1) | CN105506135B (zh) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101212251B1 (ko) * | 2010-07-14 | 2012-12-12 | 서울대학교산학협력단 | 돼지의 장내 미생물 진단용 dna 칩, 칩을 포함하는 키트 및 키트를 이용한 진단방법 |
CN104152563B (zh) * | 2014-08-18 | 2016-04-06 | 南京大学 | 一种检测污染水体中猪粪的pcr试剂盒及其检测方法 |
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Inventor after: Duan Chuanren Inventor after: Cui Yamin Inventor after: Zhang Kun Inventor after: Sun Da Inventor after: Zhang Baoyun Inventor after: Di Jun Inventor after: Zhou Fandie Inventor before: Duan Chuanren Inventor before: Cui Yamin Inventor before: Zhang Kun Inventor before: Sun Da Inventor before: Zhang Baoyun Inventor before: Di Jun |
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