CN105462983A - 一种抑制水稻OsRboh(LOC_Os01g25820)基因的amiRNA - Google Patents
一种抑制水稻OsRboh(LOC_Os01g25820)基因的amiRNA Download PDFInfo
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- CN105462983A CN105462983A CN201610028696.8A CN201610028696A CN105462983A CN 105462983 A CN105462983 A CN 105462983A CN 201610028696 A CN201610028696 A CN 201610028696A CN 105462983 A CN105462983 A CN 105462983A
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
- C12N2310/141—MicroRNAs, miRNAs
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CN201610028696.8A CN105462983B (zh) | 2016-01-18 | 2016-01-18 | 一种抑制水稻OsRboh(LOC_Os01g25820)基因的amiRNA |
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CN201610028696.8A CN105462983B (zh) | 2016-01-18 | 2016-01-18 | 一种抑制水稻OsRboh(LOC_Os01g25820)基因的amiRNA |
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CN105462983A true CN105462983A (zh) | 2016-04-06 |
CN105462983B CN105462983B (zh) | 2023-04-07 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109652443A (zh) * | 2019-02-25 | 2019-04-19 | 四川大学 | 一种人工microRNA干扰载体及其构建方法和应用 |
CN113416747A (zh) * | 2020-03-03 | 2021-09-21 | 山东舜丰生物科技有限公司 | 一种创建温度敏感型雄性不育植物的方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1671847A (zh) * | 2002-07-22 | 2005-09-21 | 巴斯福植物科学有限公司 | 在植物中获得病原体抗性的方法 |
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2016
- 2016-01-18 CN CN201610028696.8A patent/CN105462983B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1671847A (zh) * | 2002-07-22 | 2005-09-21 | 巴斯福植物科学有限公司 | 在植物中获得病原体抗性的方法 |
Non-Patent Citations (6)
Title |
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KIKUCHI ET AL.: "AK065117.1", 《GENBANK》 * |
严红: "植物人工微RNA(amiRNA)表达载体构建的方法学研究及其应用", 《中国博士学位论文全文数据库 基础科技辑》 * |
刘秋圆: "水稻OsRboh1基因的表达模式分析与遗传转化初步研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
刘秋圆等: "植物Rboh基因功能及其活性调节机制的研究进展", 《生物技术通报》 * |
李业等: "OsRboh基因家族在水稻免疫应答中的表达及功能分析", 《生物工程学报》 * |
胡丽芳等: "水稻OsRbohB基因原位杂交载体的构建及其在花药中的表达分析", 《广东农业科学》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109652443A (zh) * | 2019-02-25 | 2019-04-19 | 四川大学 | 一种人工microRNA干扰载体及其构建方法和应用 |
CN109652443B (zh) * | 2019-02-25 | 2023-04-07 | 四川大学 | 一种人工microRNA干扰载体及其构建方法和应用 |
CN113416747A (zh) * | 2020-03-03 | 2021-09-21 | 山东舜丰生物科技有限公司 | 一种创建温度敏感型雄性不育植物的方法 |
CN113416747B (zh) * | 2020-03-03 | 2023-09-12 | 山东舜丰生物科技有限公司 | 一种创建温度敏感型雄性不育植物的方法 |
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Inventor after: He Haohua Inventor after: Bian Jianmin Inventor after: Xu Jie Inventor after: Sun Xiaotang Inventor after: Zhou Dahu Inventor after: Hu Lifang Inventor after: Liu Shiqiang Inventor after: Zhu Changlan Inventor after: OuYang Linjuan Inventor after: Peng Xiaosong Inventor after: Chen Xiaorong Inventor after: He Xiaopeng Inventor after: Fu Junru Inventor before: Hu Lifang Inventor before: Bian Jianmin Inventor before: Xu Jie Inventor before: Sun Xiaotang Inventor before: Zhou Dahu Inventor before: He Haohua Inventor before: Liu Shiqiang Inventor before: Zhu Changlan Inventor before: Ouyang Linjuan Inventor before: Peng Xiaosong Inventor before: Chen Xiaorong Inventor before: He Xiaopeng Inventor before: Fu Junru |
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