CN105393908B - The method and modular combination of the quick modularization plantation of submerged plant tissue-cultured seedling - Google Patents
The method and modular combination of the quick modularization plantation of submerged plant tissue-cultured seedling Download PDFInfo
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- CN105393908B CN105393908B CN201510784766.8A CN201510784766A CN105393908B CN 105393908 B CN105393908 B CN 105393908B CN 201510784766 A CN201510784766 A CN 201510784766A CN 105393908 B CN105393908 B CN 105393908B
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- seedling
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- submerged plant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/021—Pots formed in one piece; Materials used therefor
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- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
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Abstract
The invention discloses the methods and modular combination of a kind of quick modularization plantation of submerged plant tissue-cultured seedling.Steps are as follows for this method：Aseptic seedling is turned out according to tissue culture technique, obtains the aseptic seedlings for completion of having taken root, lid hardening is then opened in constant incubator；The aseptic seedling is Haloragidaceae Myriophyllum spicatum, waterweed, hydrilla verticillata；Water tank of the cube without upper cover is chosen, dirt bed, brown bed course is laid with successively in the bottom of water tank, by the seedling replanting after hardening to brown bed course, nylon wire is laid on seedling layer, the water of the quick-fried tap water or target water body for crossing gas is introduced in water tank；Natural lighting, temperature range are 18 ~ 30 DEG C.Water body under simulating natural environment of the present invention transplants a certain number of aseptic seedlings, selects the quick modularization plantation of submerged plant seedling, and repairing aquatic vegetation for aquatic ecosystem structure bottom environment is used.Survival rate is high, is easy transport and transplanting, and can be customized collocation according to the features such as water quality of target water body and be applied in combination.
Description
Technical field
The invention belongs to young plant growers technical fields, and in particular to a kind of quick modularization kind of submerged plant tissue-cultured seedling
The method of plant.
Background technology
Tissue culture technique（Tissue Culture Technology）It is will live under sterile conditions organ, tissue
Or cell is placed in culture medium, and be placed in suitable environment, carry out cell, tissue or individual made of continuously cultivating.It is this
Technology is widely used to the research of agricultural and biology, medicine.The aseptic seedling system obtained by tissue culture technique can be wide
The general recovery applied to the ecosystem.And how the aseptic seedlings of acquisition to be successfully transferred in natural water, and ensure children
The normal growth of seedling and procreation relate to structure and the selection of substrate to gradually restore the water body environment being damaged.Heavy
During water revegetation, need to adapt to the otherness of matrix according to different submerged plants to build aquatic vegetation, to not
Certain reparation is carried out suitable for the matrix of submerged plant growth.The type of submerged plant is more, needs to adapt to base to submerged plant
Matter makes further research, and to ensure that submerged plant good can grow, and then realizes the reconstruction of lake submerged vegetation and ecosystem
The recovery of system.Therefore, suitable substrate material transformation bottom environment is selected also to be embodied in ecosystem restoration system non-same
General importance.
Invention content
For overcome the deficiencies in the prior art, the present invention provides a kind of quick modularization plantations of submerged plant tissue-cultured seedling
Method and modular combination.On the basis of tissue culture is successful, it is completely dependent on natural lighting, temperature range is 18-30 DEG C of progress, root
Best substrate combination of materials is selected according to submerged plant growth situation, the Haloragidaceae Myriophyllum spicatum at 28 days, waterweed, hydrilla verticillata is complete
Portion survives, and growth length is respectively 36cm, 35 cm, 63 cm.Easy to implement the method, easy to operate, reliability is high.
The present invention uses following technical scheme：
A kind of method of the quick modularization plantation of submerged plant tissue-cultured seedling, steps are as follows：
A, the acquisition of tissue-cultured seedling：Aseptic seedling is turned out according to tissue culture technique, obtains the aseptic seedlings for completion of having taken root,
Then lid hardening is opened in constant incubator；The aseptic seedling is Haloragidaceae Myriophyllum spicatum, waterweed, hydrilla verticillata；
B, the structure of substrate and transplanting：
Water tank of the cube without upper cover is chosen,
It is laid with dirt bed, brown bed course successively in the bottom of water tank, a height of 3cm of the dirt bed,
By on the seedling replanting after hardening to brown bed course,
It is laid with nylon wire on seedling layer,
The water that the quick-fried tap water or target water body for crossing gas is introduced in water tank, it is more than seedling layer average height to make horizontal plane
10cm accordingly supplements water with the growth of seedling；Natural lighting, temperature range are 18 ~ 30 DEG C, in the step A when hardening
Between be 3 days, the growth time of aseptic seedlings is >=15 days in the step B.
The water tank uses low-density high-pressure polyethylene (LLDPE) material, general to select completely newly, is examined according further to needs
Consider corrosion resistance, density etc. can also select other materials.
The palm fibre pad and nylon wire size are sheared according to the size of water tank bottom, make it that can just cover bottom.
The long 112cm of the water tank, width 60cm, high 64cm, the water tank are bisected into three parts, are placed respectively in every portion
22 Haloragidaceae Myriophyllum spicatums, 22 waterweeds and 22 hydrilla verticillatas aseptic seedlings.
The type or quantity of the aseptic seedling change and increase and decrease according to target water body environment.
The submerged plant seedling modular combination that a kind of method described in basis obtains, including according to target water body environment and
The type and quantity of fixed aseptic seedling, dirt bed, brown bed course, nylon mesh layer, water tank, for water-bed Eco-environmental Reconstruction.
The present invention has the following advantages and effect compared with general planting technology：
The seedling that just cultivation has come is more fragile, and good bottom environment can make it easier to survive, and grows rapidly, also not
The case where depauperation can occur in default of nutrition.The submerged plant planting method for being currently used for restoration of the ecosystem is mostly to use
Non-woven fabrics encases plant root, is thrown into water, and such plant is difficult to take root and be colonized, and plant is easy to be blown away by stormy waves, nothing
Method forms phytobiocoenose well.The present invention simulates natural water, designs substrate, by recording the growing state of seedling and surviving
Rate has selected most adaptable growing environment, and in the combination of nylon wire+palm fibre pad+soil, the survival rate of the plant reaches 100%,
One month growth length is as to shown in table 1, far surpassed the effect for planting planting submerged plant tissue-cultured seedling directly in the bed mud of natural water
Fruit can be very good to be applied in heavy water seedling reparation polluted-water environment.In addition, the present invention can form modularization laying,
It is easy transport and transplanting, and collocation can be customized according to the features such as water quality of target water body and be applied in combination.
Description of the drawings
Fig. 1 is the growing state of heavy water seedling in three kinds of bottom environments,
Note：It is Haloragidaceae Myriophyllum spicatum successively from top to bottom, waterweed, hydrilla verticillata,
Part A, nylon wire+soil；Part B, nylon wire+palm fibre pad+soil；C portion, nylon wire+non-woven fabrics+palm fibre pad+mud
Soil.
Specific implementation mode
Reagent used in the embodiment of the present invention can select to those skilled in the art if not otherwise specified
It is commercially available to realize the present invention.
Embodiment 1：
A, the acquisition of tissue-cultured seedling：Go out Haloragidaceae Myriophyllum spicatum, waterweed, wheel blade in laboratory cultures according to known experimental method
The aseptic seedling of black algae obtains complete aseptic seedlings, bottle cap hardening is then opened in constant incubator 3 days, is then ready for turning
Enter in water body environment.
B, the structure of substrate：The long 112cm wide of water tank, 60cm, the high 64cm of selection, can just according to the shearing of the size of water tank
Place mat all spreads 3 centimetres in the nylon wire of bottom, nylon wire+palm fibre pad, nylon wire+palm fibre pad+non-woven fabrics, each substrate in bottom
Soil, build the environment of different substrate combinations of materials, nylon wire waits for covering again after aseptic seedling is put into wherein.
C, hardening and transplanting：By transplanting after the whole seedlings hardening of three kinds of acquisition heavy water seedling to three kinds of different substrate rings
In border, each water tank is bisected into three parts, and 22 three kinds of heavy water seedling are uniformly placed per a interior difference.Nylon wire is covered, is added
Water is more than seedling 10cm.With the growth of seedling, while amount of makeup water, so that the water surface is consistently higher than 10 cm of plant.Heavy water seedling
Quantity is related with the water tank size of selection.
D, survival rate and growing state are recorded：Each week surveys an average length, records survival rate（Such as table 2, table 3, table
4）.
E, it summarizes：According to Haloragidaceae Myriophyllum spicatum, waterweed, hydrilla verticillata growing state and survival rate in three kinds of substrates,
The theoretical foundation for selecting optimum substrate combination of materials to be selected as tissue culture transplantation of seedlings substrate.Waterweed is most to adapt to water body soon
Environment, it is come out with most fast growth rate, and survival rate reaches 100%.Three kinds of substrates compare, C groups（Nylon wire+non-woven fabrics+
Brown pad+soil）Survival rate is minimum, and seedling is short and small, and growing way is slow, and some is grown in later death.B groups（Nylon wire+palm fibre pad+
Soil）Survival rate highest, three kinds of heavy water growth of seedling situations are also all best, and A groups（Nylon wire+soil）B groups are only second to,
But growing state is substantially better than C groups.In short, under the water body environment, the substrate combination of soil+palm fibre pad+nylon wire is most suitable for
These three heavy water growth of seedling, and survival rate reaches 100%.The combination of plant can be replaced according to different water bodys, this is this field
Known to technical staff.
Natural lighting is relied in entire planting process, temperature range is between 18-30 DEG C.
Claims (6)
1. a kind of method of the quick modularization plantation of submerged plant tissue-cultured seedling, which is characterized in that steps are as follows：
A, the acquisition of tissue-cultured seedling：Aseptic seedling is turned out according to tissue culture technique, obtains the aseptic seedlings for completion of having taken root, then
Lid hardening is opened in constant incubator；The aseptic seedling is Haloragidaceae Myriophyllum spicatum, waterweed, hydrilla verticillata；
B, the structure of substrate and transplanting：
Water tank of the cube without upper cover is chosen,
It is laid with dirt bed, brown bed course successively in the bottom of water tank, a height of 3cm of the dirt bed,
By on the seedling replanting after hardening to brown bed course,
It is laid with nylon wire on seedling layer,
The water that the tap water or target water body that exposed gas are introduced in water tank, it is more than seedling layer average height to make horizontal plane
10cm accordingly supplements water with the growth of seedling；Natural lighting, temperature range are 18 ~ 30 DEG C, in the step A when hardening
Between be 3 days, the growth time of aseptic seedlings is >=15 days in the step B.
2. according to the method described in claim 1, it is characterized in that, the water tank uses low-density high-pressure polyethylene
(LLDPE) material.
3. according to the method described in claim 1, it is characterized in that, the palm fibre pad and nylon wire size are according to water tank bottom
Size is sheared, and makes it that can just cover bottom.
4. according to the method described in claim 1, it is characterized in that, the long 112cm of the water tank, width 60cm, high 64cm, described
Water tank be bisected into three parts, per in a respectively 22 Haloragidaceae Myriophyllum spicatums of placement, 22 waterweeds and 22 hydrilla verticillatas nothing
Bacterium seedling.
5. according to the method described in claim 1, it is characterized in that, the type or quantity of the aseptic seedling are according to target water
Body environment changes and increase and decrease.
6. the submerged plant seedling modular combination that a kind of method according to claim 11 obtains, which is characterized in that including
The type and quantity of aseptic seedling depending on target water body environment, the dirt bed, brown bed course, nylon mesh layer, water tank, are used for
Water-bed Eco-environmental Reconstruction.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108901849B (en) * | 2018-07-26 | 2022-03-08 | 水生藻安生物科技（武汉）有限公司 | Tissue culture method of aseptic seedlings of hydrilla verticillata |
| CN110150043B (en) * | 2019-04-28 | 2021-05-25 | 南京中科水治理股份有限公司 | Method for cultivating and transplanting hirsutella suiffii land seedlings |
| CN110122299A (en) * | 2019-05-27 | 2019-08-16 | 水生藻安生物科技（武汉）有限公司 | It is a kind of to there is phosphorus to be enriched with and drop the water plant field planting method of nitrogen function |
| CN110352842B (en) * | 2019-07-08 | 2024-03-26 | 湖南省建筑设计院有限公司 | Submerged plant cultivation system based on fiber blanket cultivation |
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