CN105331555A - Composite agricultural microorganism bacteria and method for applying composite bacteria to lotus root planting - Google Patents

Composite agricultural microorganism bacteria and method for applying composite bacteria to lotus root planting Download PDF

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Publication number
CN105331555A
CN105331555A CN201510738349.XA CN201510738349A CN105331555A CN 105331555 A CN105331555 A CN 105331555A CN 201510738349 A CN201510738349 A CN 201510738349A CN 105331555 A CN105331555 A CN 105331555A
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bacteria
composite
mixed solution
weight
farm
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CN201510738349.XA
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任艳萍
李建飞
张远斌
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Nanping Zhengda Ouruixin Biotechnology Development Co Ltd
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Nanping Zhengda Ouruixin Biotechnology Development Co Ltd
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Priority to CN201510738349.XA priority Critical patent/CN105331555A/en
Publication of CN105331555A publication Critical patent/CN105331555A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Soil Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides composite agricultural microorganism bacteria and a method for applying composite bacteria to lotus root planting. All strains of compound bacteria comprise, by weight, 20% of rhodospirillum, 10% of nitrospira, 10% of azotobacter, 10% of bacilli, 10% of thermoactinomyces, 10% of xanthomonas, 10% of lysobacter, 10% of acidophilic bacteria and 10% of novosphingobium. The composite bacteria are used for lotus root planting, the yield of lotus roots is increased, and the quality of the lotus roots is improved.

Description

A kind of composite farm microbial bacteria and composite bacteria is used for lotus rhizome plantation method
Technical field
The present invention relates to agriculture field, particularly relate to a kind of composite farm microbial bacteria and this composite bacteria is used for the method for lotus rhizome plantation.
Background technology
Existing lotus rhizome planting technology is usually through water table management, weeding, the making for improving its output of chemical agent and fertilizer, but the use of chemical fertilizer makes lotus rhizome quality effectively do not improved.Improving lotus rhizome output, oil recovery enhancement though also have by genetic engineering means, also there is many uncertainties in this technology, is not also employed to promote.
Also have many microniological proudcts at present, but its flora kind is single or the composite prod of several bacterium, the bacterial diversity of microniological proudcts is not enough.Using routine techniques to improve yield and quality needs the manpower and materials of input large, and the use of fertilizer and pesticide easily produces pollution, causes soil compaction, and soil property declines.
Summary of the invention
The technical problem to be solved in the present invention, be to provide a kind of composite farm microbial bacteria, described composite bacteria comprises following bacterial classification: rhodospirillum, nitrated spirillum, vinelandii, genus bacillus, thermophilic actinomycete, Xanthomonas campestris, molten bacillus, acidophilic bacteria, new sphingolipid bacterium.
Further, the weight percent of each bacterial classification of described composite bacteria is: rhodospirillum 20%, nitrated spirillum 10%, vinelandii 10%, genus bacillus 10%, thermophilic actinomycete 10%, Xanthomonas campestris 10%, molten bacillus 10%, acidophilic bacteria 10%, new sphingolipid bacterium 10%.
Further, the preparation method of described composite bacteria comprises the following steps:
1, choose each bacterial classification and mix by described part by weight after cultivating separately;
2, wheat bran, dregs of beans, molasses and the mixed complex liquid of described composite bacteria liquid are carried out fermentation culture;
3, complex liquid is cultured to moisture be down to 30% and living bacteria count is greater than 1 × 109cfu/g time, stop cultivate and carry out vacuum packaging.
4. composite farm microbial bacteria according to claim 3, is characterized in that, in described step 1, the part by weight of wheat bran, dregs of beans, molasses and described composite bacteria liquid is 6:2:1:1.
Further, described composite farm microbial bacteria is used for the method for lotus rhizome plantation, comprises the following steps:
1, complex liquid, honey, water are mixed by the part by weight of 1:1:200, expose to the sun mixed solution oxygen 7 days continuously;
2, the mixed solution application with irrigation water after oxygen of step 1 being exposed to the sun is discharged in lotus root Tanaka;
3, after 2 ~ 4 weeks, by complex liquid, honey, water is by the half weight taking relative step 1 and mix by the part by weight of 1:1:200, and expose to the sun mixed solution oxygen 3 days continuously;
4, the mixed solution application with irrigation water after oxygen of step 3 being exposed to the sun is discharged in lotus root Tanaka.
Further, the usage quantity of described mixed solution is 100 ~ 140 kgs/acre, preferably 120.12 kgs/acre.
The present invention has following advantage:
1, species used herein enriches, and easy to use, natural environmental protection, can better adapt to various environment, its effect of fast onset;
2, do not need to continue for a long time to add bacterial classification, can play and continue improvement lotus root field, improve lotus rhizome quality and improve the effect of output;
3, the probiotics used is composite bacteria, active high, strong to adaptive capacity to environment, and adopt the plain modes such as irrigation or fertilising to add, do not need to increase other cost, labour cost is low.
Embodiment
A kind of composite farm microbial bacteria of most preferred embodiment of the present invention, the weight percent of each bacterial classification is: rhodospirillum 20%, nitrated spirillum 10%, vinelandii 10%, genus bacillus 10%, thermophilic actinomycete 10%, Xanthomonas campestris 10%, molten bacillus 10%, acidophilic bacteria 10%, new sphingolipid bacterium 10%.
The preparation method of described composite bacteria comprises the following steps:
1, choose each bacterial classification and mix by described part by weight after cultivating separately;
2, be the wheat bran of 6:2:1:1 by part by weight, dregs of beans, molasses and the mixed complex liquid of described composite bacteria liquid carry out fermentation culture;
3, complex liquid is cultured to moisture be down to 30% and living bacteria count is greater than 1 × 109cfu/g time, stop cultivate and carry out vacuum packaging.
4. composite farm microbial bacteria according to claim 3, is characterized in that, in described step 1, wheat bran, dregs of beans, molasses and described composite bacteria liquid.
Described composite farm microbial bacteria is used for the application method of lotus rhizome plantation, comprises the following steps:
1, according to concrete lotus root field size, complex liquid, honey, water are mixed by the part by weight of 1:1:200, take mixed solution according to the gross weight of 120.12 kgs/acre, expose to the sun mixed solution oxygen 7 days continuously;
2, the mixed solution application with irrigation water after oxygen of step 1 being exposed to the sun is discharged in lotus root Tanaka;
3, after 2 ~ 4 weeks, by complex liquid, honey, water is by the half weight taking relative step 1 and mix by the part by weight of 1:1:200, and expose to the sun mixed solution oxygen 3 days continuously;
4, the mixed solution application with irrigation water after oxygen of step 3 being exposed to the sun is discharged in lotus root Tanaka.
The specific embodiment that utilization aforesaid method carries out lotus rhizome plantation is as follows: composite microbial bacteria is applied to lotus root field, arranges the control group not executing bacterial manure simultaneously.
(1) test materials: lotus rhizome.
(2) test process: test establishes 2 process, experimental group and control group, and the cultivated area of experimental group and control group is all made up of 3.33 mu and 3.77 mu of two pieces of lands for growing field crops.
(3) test method: altogether discharge twice.First day, takes bacterial classification 2.0 kilograms and is watered 400 kilograms, and expose to the sun after the mixing of 2.0 kilograms, honey oxygen 7 days continuously, and within the 8th day, discharge first time, application with irrigation water is discharged.17 day, take bacterial classification 0.5 kilogram and be watered 100 kilograms, expose to the sun after the mixing of 0.5 kilogram, honey oxygen 3 days continuously, and Ahau discharges second time, and application with irrigation water is discharged.
(4) survey item: main lotus root quality, diameter and coat color, vitamins C, reducing sugar, robust fibre, nitrate content.
(5) interpretation of result: experimental group lotus rhizome commodity is good: lotus root shape is full, ripening degree foot, epidermis are smooth.Output is significantly higher than control group.The reducing sugar of experimental group lotus rhizome, ascorbic content are higher than control group, and robust fibre, nitrate content are significantly lower than control group.The lotus rhizome of experimental group gives birth to crisp few cinder notch sense because content of cellulose is low good, and because of VITAMIN, reducing sugar content is high and taste is sweet is of high nutritive value, the edible safety because nitrate content is low.Concrete numerical result is as shown in table 1.
Table 1 lotus rhizome Yield and qualities detection case
In the present invention, its activity of compound probiotics of abundant species is high, accommodative ability of environment is strong, the ecotope of lotus root field different steps is different, amount reproduction can be adapted to the population of this environment by the probiotics that can overcome poor environment impact in composite bacteria fast, and form dominant population, thus under not needing to continue for a long time to think and increasing beneficial microorganism situation, increase the quantity of lotus root field probiotics, define complicated and stable microecosystem, improve field microenvironment, form the good environment being suitable for plant-growth, simultaneously, also generation is easily the benefit materials of plant absorption in a large number, as amino acid, organic acid, polyose, various VITAMIN, various biochemical enzyme, somatomedin, microbiotic and antiviral substance etc., improve the immunologic function of plant, promotion health grows.
Photosynthetic bacteria group wherein utilizes sun power, synthesis carbohydrate, amino acid, vitamins, nitrogen compound and physiologically active substance etc.; Yeast flora utilizes amino acid, carbohydrate and other organic substance, by fermentation, produces and promotes fissional active substance, for other effective microbe propagation provides important nutrition assurance, and can prevent the generation of maggot and other insects; Actinomycete group produces various antibiotics, VITAMIN and enzyme, directly suppresses pathogenic bacteria, strengthens the resistance against diseases of crop.Microorganism decomposes promotion root system absorbing nutrient to macromolecular substance, and the interaction of various effective microbe promotes the raising of lotus rhizome output and the improvement of quality.
Above by reference to the accompanying drawings to invention has been exemplary description; obvious specific implementation of the present invention is not subject to the restrictions described above; as long as have employed the improvement of the various unsubstantialities that method of the present invention is conceived and technical scheme is carried out; or design of the present invention and technical scheme directly applied to other occasion, all within protection scope of the present invention without to improve.

Claims (7)

1. a composite farm microbial bacteria, is characterized in that, described composite bacteria comprises following bacterial classification: rhodospirillum, nitrated spirillum, vinelandii, genus bacillus, thermophilic actinomycete, Xanthomonas campestris, molten bacillus, acidophilic bacteria, new sphingolipid bacterium.
2. composite farm microbial bacteria according to claim 1, it is characterized in that, the weight percent of each bacterial classification of described composite bacteria is: rhodospirillum 20%, nitrated spirillum 10%, vinelandii 10%, genus bacillus 10%, thermophilic actinomycete 10%, Xanthomonas campestris 10%, molten bacillus 10%, acidophilic bacteria 10%, new sphingolipid bacterium 10%.
3. composite farm microbial bacteria according to claim 2, is characterized in that, the preparation method of described composite bacteria comprises the following steps:
1, choose each bacterial classification and obtain composite bacteria liquid by the mixing of described part by weight after cultivating separately;
2, wheat bran, dregs of beans, molasses and the mixed complex liquid of described composite bacteria liquid are carried out fermentation culture;
3, complex liquid is cultured to moisture be down to 30% and living bacteria count is greater than 1 × 109cfu/g time, stop cultivate and carry out vacuum packaging.
4. composite farm microbial bacteria according to claim 3, is characterized in that, in described step 1, the part by weight of wheat bran, dregs of beans, molasses and described composite bacteria liquid is 6:2:1:1.
5. composite farm microbial bacteria is used for a method for lotus rhizome plantation, it is characterized in that, the application method composite bacteria described in described claim 3 or 4 being used for lotus rhizome plantation comprises the following steps:
1, described complex liquid, honey, water are mixed by the part by weight of 1:1:200, expose to the sun mixed solution oxygen 7 days continuously;
2, the mixed solution application with irrigation water after oxygen of step 1 being exposed to the sun is discharged in lotus root Tanaka;
3, after 2 ~ 4 weeks, by complex liquid, honey, water is by the half weight taking relative step 1 and mix by the part by weight of 1:1:200, and expose to the sun mixed solution oxygen 3 days continuously;
4, the mixed solution application with irrigation water after oxygen of step 3 being exposed to the sun is discharged in lotus root Tanaka.
6. composite farm microbial bacteria according to claim 5, is characterized in that, the usage quantity of described mixed solution is 100 ~ 140 kgs/acre.
7. composite farm microbial bacteria according to claim 6, is characterized in that, the usage quantity of described mixed solution is 120.12 kgs/acre.
CN201510738349.XA 2015-11-03 2015-11-03 Composite agricultural microorganism bacteria and method for applying composite bacteria to lotus root planting Pending CN105331555A (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106116942A (en) * 2016-06-21 2016-11-16 农卫腾 A kind of agricultural microbial agent and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106116942A (en) * 2016-06-21 2016-11-16 农卫腾 A kind of agricultural microbial agent and preparation method thereof

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