CN105283550A - 生物样品的稳定化 - Google Patents

生物样品的稳定化 Download PDF

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Publication number
CN105283550A
CN105283550A CN201480025870.2A CN201480025870A CN105283550A CN 105283550 A CN105283550 A CN 105283550A CN 201480025870 A CN201480025870 A CN 201480025870A CN 105283550 A CN105283550 A CN 105283550A
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CN
China
Prior art keywords
sample
cell
stabilization
nucleic acid
stabilizing
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Pending
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CN201480025870.2A
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English (en)
Chinese (zh)
Inventor
拉尔夫·怀里希
托斯滕·沃斯
凯利·京特
乌伟·厄尔米勒
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Qiagen GmbH
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Qiagen GmbH
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Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of CN105283550A publication Critical patent/CN105283550A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201480025870.2A 2013-03-18 2014-03-18 生物样品的稳定化 Pending CN105283550A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361803107P 2013-03-18 2013-03-18
US61/803,107 2013-03-18
EP13160896 2013-03-25
EP13160896.0 2013-03-25
EP13180130.0 2013-08-12
EP13180130 2013-08-12
PCT/EP2014/000725 WO2014146781A1 (en) 2013-03-18 2014-03-18 Stabilisation of biological samples

Publications (1)

Publication Number Publication Date
CN105283550A true CN105283550A (zh) 2016-01-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480025870.2A Pending CN105283550A (zh) 2013-03-18 2014-03-18 生物样品的稳定化

Country Status (5)

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US (2) US11525155B2 (enExample)
EP (2) EP2976424B1 (enExample)
JP (2) JP6381628B2 (enExample)
CN (1) CN105283550A (enExample)
WO (1) WO2014146781A1 (enExample)

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CN113388606A (zh) 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
US20140227688A1 (en) 2011-09-26 2014-08-14 Qiagen Gmbh Stabilisation and isolation of extracellular nucleic acids
CN104755628B (zh) 2012-09-25 2019-03-01 凯杰有限公司 生物样品的稳定化
CN105121643A (zh) 2013-03-18 2015-12-02 凯杰有限公司 细胞外核酸的稳定化和分离
CA2940692C (en) 2014-03-18 2025-07-08 Qiagen Gmbh STABILIZATION AND ISOLATION OF EXTRACELLULAR NUCLEIC ACIDS
CN107430105A (zh) * 2015-02-09 2017-12-01 阿波根有限公司 用于采集相关应用、分析和诊断的样本的设备、溶液和方法
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JP7166169B2 (ja) 2015-11-20 2022-11-07 キアゲン ゲーエムベーハー 細胞外核酸の滅菌のための滅菌組成物を調製する方法
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JP2021506308A (ja) * 2017-12-19 2021-02-22 ザ・ウイスター・インステイテユート・オブ・アナトミー・アンド・バイオロジー 遺伝子発現プロファイルを使用して肺癌を診断するための組成物および方法
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US20160348151A1 (en) 2016-12-01
JP2016514464A (ja) 2016-05-23
EP2976424B1 (en) 2018-10-03
US11525155B2 (en) 2022-12-13
JP2018166519A (ja) 2018-11-01
EP2976424A1 (en) 2016-01-27
WO2014146781A1 (en) 2014-09-25
US20220364149A1 (en) 2022-11-17
JP6750178B2 (ja) 2020-09-02
EP3447141A1 (en) 2019-02-27
JP6381628B2 (ja) 2018-08-29
EP3447141B1 (en) 2020-08-05

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