CN105283550A - 生物样品的稳定化 - Google Patents

生物样品的稳定化 Download PDF

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Publication number
CN105283550A
CN105283550A CN201480025870.2A CN201480025870A CN105283550A CN 105283550 A CN105283550 A CN 105283550A CN 201480025870 A CN201480025870 A CN 201480025870A CN 105283550 A CN105283550 A CN 105283550A
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CN
China
Prior art keywords
sample
cell
stabilization
nucleic acid
stabilizing
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Pending
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CN201480025870.2A
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English (en)
Chinese (zh)
Inventor
拉尔夫·怀里希
托斯滕·沃斯
凯利·京特
乌伟·厄尔米勒
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Qiagen GmbH
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Qiagen GmbH
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Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of CN105283550A publication Critical patent/CN105283550A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201480025870.2A 2013-03-18 2014-03-18 生物样品的稳定化 Pending CN105283550A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361803107P 2013-03-18 2013-03-18
US61/803,107 2013-03-18
EP13160896.0 2013-03-25
EP13160896 2013-03-25
EP13180130 2013-08-12
EP13180130.0 2013-08-12
PCT/EP2014/000725 WO2014146781A1 (en) 2013-03-18 2014-03-18 Stabilisation of biological samples

Publications (1)

Publication Number Publication Date
CN105283550A true CN105283550A (zh) 2016-01-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480025870.2A Pending CN105283550A (zh) 2013-03-18 2014-03-18 生物样品的稳定化

Country Status (5)

Country Link
US (2) US11525155B2 (enExample)
EP (2) EP3447141B1 (enExample)
JP (2) JP6381628B2 (enExample)
CN (1) CN105283550A (enExample)
WO (1) WO2014146781A1 (enExample)

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EP2761020B1 (en) 2011-09-26 2018-06-13 Qiagen GmbH Stabilisation and isolation of extracellular nucleic acids
JP6608280B2 (ja) 2012-09-25 2019-11-20 キアゲン ゲーエムベーハー 生物学的試料の安定化
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WO2015140218A1 (en) 2014-03-18 2015-09-24 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
US12038434B2 (en) 2015-02-09 2024-07-16 DNA Genotek, Inc. Devices, solutions and methods for sample collection related applications, analysis and diagnosis
EP3307886B1 (en) 2015-06-10 2021-03-24 Qiagen GmbH Method for isolating extracellular nucleic acids using anion exchange particles
WO2017085321A1 (en) 2015-11-20 2017-05-26 Qiagen Gmbh Method of preparing sterilized compositions for stabilization of extracellular nucleic acids
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US11767522B2 (en) 2016-05-27 2023-09-26 Norgen Biotek Corp. Preservation of cell-free nucleic acids in biological samples
US10870891B2 (en) 2017-01-05 2020-12-22 Biodesix, Inc. Diagnostic test system for specific, sensitive and reproducible detection of circulating nucleic acids in whole blood
WO2019126343A1 (en) * 2017-12-19 2019-06-27 The Wistar Institute Of Anatomy And Biology Compositions and methods for diagnosing lung cancers using gene expression profiles
JP7548904B2 (ja) 2019-01-04 2024-09-10 キアゲン ゲーエムベーハー 尿安定化
CN114450402A (zh) * 2019-09-24 2022-05-06 恰根有限公司 稳定化的含细胞体液样品的多模式分析
EP4477751A1 (en) 2023-06-16 2024-12-18 QIAGEN GmbH Body fluid stabilization and processing

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JP6381628B2 (ja) 2018-08-29
EP3447141B1 (en) 2020-08-05
JP2016514464A (ja) 2016-05-23
JP6750178B2 (ja) 2020-09-02
EP2976424A1 (en) 2016-01-27
JP2018166519A (ja) 2018-11-01
US20220364149A1 (en) 2022-11-17
US11525155B2 (en) 2022-12-13
EP2976424B1 (en) 2018-10-03
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