JP6381628B2 - 生物試料の安定化 - Google Patents

生物試料の安定化 Download PDF

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Publication number
JP6381628B2
JP6381628B2 JP2016503571A JP2016503571A JP6381628B2 JP 6381628 B2 JP6381628 B2 JP 6381628B2 JP 2016503571 A JP2016503571 A JP 2016503571A JP 2016503571 A JP2016503571 A JP 2016503571A JP 6381628 B2 JP6381628 B2 JP 6381628B2
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JP
Japan
Prior art keywords
sample
cell
nucleic acid
cells
stabilization
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Japanese (ja)
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JP2016514464A (ja
JP2016514464A5 (enExample
Inventor
ラルフ ヴュリヒ,
ラルフ ヴュリヒ,
トルステン フォス,
トルステン フォス,
カレ ギュンター,
カレ ギュンター,
ウーヴェ アールミューラー,
ウーヴェ アールミューラー,
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Qiagen GmbH
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Qiagen GmbH
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Publication of JP2016514464A5 publication Critical patent/JP2016514464A5/ja
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2016503571A 2013-03-18 2014-03-18 生物試料の安定化 Active JP6381628B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361803107P 2013-03-18 2013-03-18
US61/803,107 2013-03-18
EP13160896 2013-03-25
EP13160896.0 2013-03-25
EP13180130 2013-08-12
EP13180130.0 2013-08-12
PCT/EP2014/000725 WO2014146781A1 (en) 2013-03-18 2014-03-18 Stabilisation of biological samples

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018143839A Division JP6750178B2 (ja) 2013-03-18 2018-07-31 生物試料の安定化

Publications (3)

Publication Number Publication Date
JP2016514464A JP2016514464A (ja) 2016-05-23
JP2016514464A5 JP2016514464A5 (enExample) 2017-02-16
JP6381628B2 true JP6381628B2 (ja) 2018-08-29

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JP2016503571A Active JP6381628B2 (ja) 2013-03-18 2014-03-18 生物試料の安定化
JP2018143839A Active JP6750178B2 (ja) 2013-03-18 2018-07-31 生物試料の安定化

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JP2018143839A Active JP6750178B2 (ja) 2013-03-18 2018-07-31 生物試料の安定化

Country Status (5)

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US (2) US11525155B2 (enExample)
EP (2) EP3447141B1 (enExample)
JP (2) JP6381628B2 (enExample)
CN (1) CN105283550A (enExample)
WO (1) WO2014146781A1 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2574956T3 (es) 2011-09-26 2016-06-23 Preanalytix Gmbh Estabilización y aislamiento de ácidos nucleicos extracelulares
AU2012314514B2 (en) 2011-09-26 2018-03-15 Qiagen Gmbh Stabilisation and isolation of extracellular nucleic acids
ES2693672T3 (es) 2011-09-26 2018-12-13 Qiagen Gmbh Método rápido para aislar ácidos nucleicos extracelulares
US11021733B2 (en) 2011-09-26 2021-06-01 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
CA2884915C (en) 2012-09-25 2022-05-17 Qiagen Gmbh Stabilisation of biological samples
WO2014146780A1 (en) 2013-03-18 2014-09-25 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
US20160374330A1 (en) 2014-03-18 2016-12-29 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
CN114965989A (zh) * 2015-02-09 2022-08-30 Dna吉诺特克股份有限公司 用于采集相关应用、分析和诊断的样本的设备、溶液和方法
US11104896B2 (en) 2015-06-10 2021-08-31 Qiagen Gmbh Method for isolating extracellular nucleic acids using anion exchange particles
CA3005694A1 (en) * 2015-11-20 2017-05-26 Qiagen Gmbh Method of preparing sterilized compositions for stabilization of extracellular nucleic acids
PL3205722T3 (pl) * 2016-02-11 2019-05-31 Sarstedt Ag & Co Kg Urządzenie oraz sposób izolacji kwasów nukleinowych z krwi pełnej
CA3025569A1 (en) 2016-05-27 2017-11-30 Norgen Biotek Corp. Preservation of cell-free nucleic acids in biological samples
EP3358020B1 (en) 2017-01-05 2021-05-19 Biodesix, Inc. Diagnostic test system for specific, sensitive and reproducible detection of circulating nucleic acids in whole blood
WO2019126343A1 (en) * 2017-12-19 2019-06-27 The Wistar Institute Of Anatomy And Biology Compositions and methods for diagnosing lung cancers using gene expression profiles
EP3905881A1 (en) 2019-01-04 2021-11-10 QIAGEN GmbH Urine stabilization
CA3148731A1 (en) 2019-09-24 2021-04-01 Qiagen Gmbh Multimodal analysis of stabilized cell-containing bodily fluid samples
EP4477751A1 (en) 2023-06-16 2024-12-18 QIAGEN GmbH Body fluid stabilization and processing

Family Cites Families (105)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3389133A (en) 1966-08-10 1968-06-18 Schwarz Biores Inc Process for isolating and purifying soluble ribonucleic acid
US3903179A (en) 1973-10-15 1975-09-02 Gulf Research Development Co Method for preparing nitrocyclopropane from 3-chloro-1-nitropropane
JPS6083581A (ja) * 1983-10-13 1985-05-11 Hideaki Yamada シユ−ドモナス属細菌の培養法
US4938961A (en) 1989-04-28 1990-07-03 Geoffrey Collins Organ preservation solution containing pokyethylene gycol and method of performing cardioplegia
US5460797A (en) 1991-05-08 1995-10-24 Streck Laboratories, Inc. Method for fixing tissues and cells for analysis using oxazolidine compounds
US5459073A (en) 1991-05-08 1995-10-17 Streck Laboratories, Inc. Method and composition for preserving antigens and process for utilizing cytological material produced by same
GB9215002D0 (en) 1992-07-15 1992-08-26 Univ Singapore Method of fixing biological tissue
DK0743950T3 (da) 1994-02-11 2002-03-25 Qiagen Gmbh Fremgangsmåde til adskillelse af dobbeltstrengede/enkeltstrengede nukleinsyre-strukturer
ES2459290T3 (es) 1994-08-11 2014-05-08 Sanofi-Aventis Deutschland Gmbh Anticuerpos monoclonales para la determinación inmunológica selectiva de formas de lamininas de alto peso molecular en líquidos corporales
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5817470A (en) 1995-03-10 1998-10-06 Sociedad Biotecnologica Collico Limitada Immobilization of antigens to solid support by the mussel adhesive polyphenolic protein and the method for use therein
US20050158699A1 (en) 1995-06-07 2005-07-21 Kadkade Prakash G. Cryopreservation of plant cells
US6673364B1 (en) 1995-06-07 2004-01-06 The University Of British Columbia Liposome having an exchangeable component
ES2235225T3 (es) 1996-02-14 2005-07-01 Biomerieux B.V. Aislamiento de acidos nucleicos de ramalles simples.
US6156504A (en) 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
ES2347854T5 (es) 1996-03-26 2014-07-24 Michael S. Kopreski Método que permite el uso de RNA extracelular extraído de plasma o suero para detectar, monitorizar o evaluar un cáncer
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
US6077232A (en) * 1996-12-27 2000-06-20 Sherwood Services Ag Anticoagulant composition
WO1998041651A1 (en) 1997-03-18 1998-09-24 Hsc Research & Development Limited Partnership Method for preparing chromatin
DE19726362A1 (de) 1997-06-21 1998-12-24 Schaeffler Waelzlager Ohg Vorrichtung zum Verändern der Steuerzeiten von Gaswechselventilen einer Brennkraftmaschine
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6479267B1 (en) 1998-06-17 2002-11-12 Amersham Pharmacia Biotech, Inc. FY7 polymerase
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US6407107B1 (en) 1999-05-28 2002-06-18 The Board Of Trustees Of The Unversity Of Arkansas Derivatives of butyric acid and uses thereof
US6379930B1 (en) 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails
US6492103B1 (en) 2000-01-31 2002-12-10 Organ Recovery Systems, Inc. System for organ and tissue preservation and hypothermic blood substitution
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
CN1427729A (zh) 2000-03-23 2003-07-02 科利昂特有限公司 生物材料消毒方法
US7033810B2 (en) * 2000-04-18 2006-04-25 Amgen Inc. Sitosterolemia susceptibility gene (SSG): compositions and methods of use
DE10031236A1 (de) 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
WO2002066993A1 (en) 2001-02-16 2002-08-29 Cortex Biochem, Inc. Magnetic isolation and purification of nucleic acids
JP2005501236A (ja) 2001-08-23 2005-01-13 イムニベスト・コーポレイション 分析用の細胞および生物学的検体の安定化
US20030064000A1 (en) 2001-09-24 2003-04-03 Wilson Burgess Methods of sterilizing biological mixtures using stabilizer mixtures
DK1442045T3 (da) 2001-11-06 2009-02-09 Cortex Biochem Inc Isolering og oprensning af nukleinsyrer
US6954128B2 (en) 2001-11-30 2005-10-11 The Regents Of The University Of California High performance hybrid magnetic structure for biotechnology applications
US6977162B2 (en) 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
WO2003086480A1 (en) 2002-04-10 2003-10-23 Clearant, Inc. Methods for sterilizing biological materials
JP2005524850A (ja) 2002-05-07 2005-08-18 ベクトン・ディキンソン・アンド・カンパニー 採集装置
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20040054160A1 (en) * 2002-09-16 2004-03-18 Santona Pal Nucleic-acid ink compositions for arraying onto a solid support
JP2006502415A (ja) 2002-10-10 2006-01-19 ベクトン・ディキンソン・アンド・カンパニー カスパーゼ阻害剤を有するサンプル収集システム
EP1580554A4 (en) 2002-12-02 2006-03-15 Toyo Kohan Co Ltd PROCESS FOR IMMOBILIZING POLYNUCLEOTIDE ON A SOLID CARRIER, FIXED IMMOBILIZING CARRIED ACCORDING TO SAID METHOD, PROCESS FOR DETECTING A TARGET POLYNUCLEOTIDE FROM THE SOLID SUPPORT AND SOLUTION FOR IMMOBILIZING POLYNUCLEOTIDE
US20040167165A1 (en) 2003-01-16 2004-08-26 Geetha Shankar Methods of treating conditions associated with an Edg-7 receptor
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US8758974B2 (en) 2003-03-27 2014-06-24 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Photoresist-free micropatterning on polymer surfaces
US7250270B2 (en) 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction
EP1510577A1 (en) 2003-08-29 2005-03-02 Qiagen GmbH Method for magnetic bead isolation of nucleic acids
US20070208166A1 (en) 2003-10-24 2007-09-06 Exelixis, Inc. Tao Kinase Modulators And Method Of Use
US7270799B2 (en) 2004-01-15 2007-09-18 Nst Neurosurvival Technologies Ltd. Perturbed membrane-binding compounds and methods of using the same
WO2005081867A2 (en) 2004-02-20 2005-09-09 The Regents Of The University Of C Alifornia Salivary mrna profiling, biomarkers, and related methods and kits of parts
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
JP2008509226A (ja) 2004-05-24 2008-03-27 ジェンボールト コーポレイション 回収可能な形式での安定なタンパク質保管および安定な核酸保管
US20100190688A1 (en) 2004-07-12 2010-07-29 Bin Chao Tetrapeptide analogs
US7727718B2 (en) 2005-01-04 2010-06-01 Molecular Research Center, Inc. Reagents for storage and preparation of samples for DNA analysis
EP1859038B1 (en) 2005-02-11 2010-08-04 Invitrogen Dynal AS Method for isolating nucleic acids comprising the use of ethylene glycol multimers
FR2883003B1 (fr) 2005-03-14 2008-04-11 Aldivia Sa Nouveaux antioxydants a base d'especes d'anacardiacees, plus particulierement de sclerocarya birrea, leurs procedes d'obtention et leurs applications
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
AU2011253662A1 (en) 2005-11-30 2011-12-22 Massachusetts Institute Of Technology Pathogen-detecting cell preservation systems
EP1804045B1 (de) 2005-12-30 2014-03-12 QIAGEN GmbH Ein Verfahren und ein Kit zur Behandlung einer biologischen Probe
CA2635978A1 (en) 2006-01-04 2007-07-12 Do-Coop Technologies Ltd. Solid-fluid composition
CA2637843C (en) 2006-01-20 2014-12-30 Reneuron, Inc. Method of improving cell proliferation of pancreatic progenitor cells in a pancreatic cell culture
US20070190535A1 (en) 2006-02-13 2007-08-16 Hall Gerald E Jr Size fractionation of nucleic acid samples
CA2550133A1 (en) 2006-02-16 2007-08-16 The Governors Of The University Of Alberta Temperature regulated gene expression
JP5274781B2 (ja) 2007-03-22 2013-08-28 昭和電工株式会社 R−t−b系合金及びr−t−b系合金の製造方法、r−t−b系希土類永久磁石用微粉、r−t−b系希土類永久磁石
US7828887B2 (en) 2007-04-23 2010-11-09 Hewlett-Packard Development Company, L.P. Dye-based ink formulations
DE102007025277A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Verfahren zur Stabilisierung einer biologischen Probe
US7960098B2 (en) 2007-07-12 2011-06-14 Warsaw Orthoperic, Inc. Methods and compositions for the preservation of cells and tissues
US7892724B2 (en) 2007-07-12 2011-02-22 Warsaw Orthopedic, Inc Method for enhancing the viability of mammalian cells, tissues and organs using a solution comprising two low molecular weight PEGs
EP2027922A1 (de) 2007-08-02 2009-02-25 Qiagen GmbH Verfahren und Vorrichtung für das Fixieren/Stabilisieren einer Probe
US20100285468A1 (en) 2007-09-24 2010-11-11 Allelogic Biosciences Corporation Detection and/or quantification of nucleic acids
EP2259798B1 (en) 2008-03-05 2013-12-11 Baxter International Inc. Surface-modified particles and methods for targeted drug delivery
US20100137575A1 (en) 2008-12-03 2010-06-03 Connolly D Michael Universal biological sample processing
US11634747B2 (en) 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
EP3290530B1 (en) 2009-02-18 2020-09-02 Streck Inc. Preservation of cell-free nucleic acids
US20100280233A1 (en) 2009-05-04 2010-11-04 Connolly Dennis M Method for sample preparation
US20150056604A1 (en) 2009-06-15 2015-02-26 Biovec Transfusion, Llc Compositions and methods for preserving platelet function
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
TWI407994B (zh) 2009-10-22 2013-09-11 Ind Tech Res Inst 分離核酸的方法、試劑及套組
NZ599583A (en) 2009-10-29 2013-09-27 Ascendis Pharma As Sterilization of biodegradable hydrogels
JP2013509883A (ja) * 2009-11-06 2013-03-21 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 臓器移植患者における移植片拒絶反応の非侵襲的診断方法
WO2011057184A1 (en) * 2009-11-09 2011-05-12 Streck, Inc. Stabilization of rna in and extracting from intact cells within a blood sample
JP5773347B2 (ja) 2009-11-27 2015-09-02 国立大学法人 千葉大学 血液細胞の凍結保存剤
US8093427B2 (en) 2010-06-09 2012-01-10 National Tsing Hua University Construction and screening of solution-phase derived library of fenbufen and ethacrynic acid
US20130095473A1 (en) 2010-06-14 2013-04-18 Qiagen Gmbh Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
FR2964567B1 (fr) 2010-09-14 2013-03-15 Lvmh Rech Extrait d'orchidee
ES2668213T3 (es) 2011-05-03 2018-05-17 Agios Pharmaceuticals, Inc. Uso de activadores de piruvato quinasa para aumentar la vida útil de los glóbulos rojos de la sangre y tratar la anemia
US9664702B2 (en) * 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US11021733B2 (en) * 2011-09-26 2021-06-01 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
ES2574956T3 (es) * 2011-09-26 2016-06-23 Preanalytix Gmbh Estabilización y aislamiento de ácidos nucleicos extracelulares
AU2012314514B2 (en) * 2011-09-26 2018-03-15 Qiagen Gmbh Stabilisation and isolation of extracellular nucleic acids
ES2693672T3 (es) 2011-09-26 2018-12-13 Qiagen Gmbh Método rápido para aislar ácidos nucleicos extracelulares
EP2760999A1 (en) 2011-09-26 2014-08-06 Qiagen GmbH Methods for separating nucleic acids by size
US9994891B2 (en) 2011-10-11 2018-06-12 Qiagen Gmbh Sample processing method and sample processing cartridge
CA2855941A1 (en) 2011-11-14 2013-05-23 Advanced Cell Technology, Inc. Pharmaceutical preparations of human rpe cells and uses thereof
US9546358B2 (en) 2012-06-04 2017-01-17 New England Biolabs, Inc. Compositions and methods for reducing background DNA amplification
CA2884915C (en) 2012-09-25 2022-05-17 Qiagen Gmbh Stabilisation of biological samples
WO2014055936A1 (en) 2012-10-04 2014-04-10 Integenx Inc. Preservation of biological materials in non-aqueous fluid media
GB201303666D0 (en) 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
WO2014146780A1 (en) * 2013-03-18 2014-09-25 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
US20160374330A1 (en) 2014-03-18 2016-12-29 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
CN106796219B (zh) 2014-08-07 2022-05-27 通用医疗公司 全血样品的稳定化

Also Published As

Publication number Publication date
EP3447141A1 (en) 2019-02-27
US11525155B2 (en) 2022-12-13
EP3447141B1 (en) 2020-08-05
JP2016514464A (ja) 2016-05-23
EP2976424B1 (en) 2018-10-03
US20220364149A1 (en) 2022-11-17
US20160348151A1 (en) 2016-12-01
JP2018166519A (ja) 2018-11-01
EP2976424A1 (en) 2016-01-27
JP6750178B2 (ja) 2020-09-02
WO2014146781A1 (en) 2014-09-25
CN105283550A (zh) 2016-01-27

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