CN105247037A - 使用重组酵母全细胞的猪圆环病毒(pcv2)亚单位疫苗及其制造方法 - Google Patents

使用重组酵母全细胞的猪圆环病毒(pcv2)亚单位疫苗及其制造方法 Download PDF

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Publication number
CN105247037A
CN105247037A CN201480026972.6A CN201480026972A CN105247037A CN 105247037 A CN105247037 A CN 105247037A CN 201480026972 A CN201480026972 A CN 201480026972A CN 105247037 A CN105247037 A CN 105247037A
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CN
China
Prior art keywords
orf2
yeast cells
bacterial strain
circular ring
ring virus
Prior art date
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Pending
Application number
CN201480026972.6A
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English (en)
Chinese (zh)
Inventor
崔毅星
朴景民
徐成和
安廷梧
李殷教
金千锡
尹寅重
俞成植
沈宁贞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
CHOONG ANG VACCINE LAB
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
CHOONG ANG VACCINE LAB
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Filing date
Publication date
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB, CHOONG ANG VACCINE LAB filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Publication of CN105247037A publication Critical patent/CN105247037A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
CN201480026972.6A 2013-03-11 2014-03-11 使用重组酵母全细胞的猪圆环病毒(pcv2)亚单位疫苗及其制造方法 Pending CN105247037A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020130025823A KR102217387B1 (ko) 2013-03-11 2013-03-11 재조합 효모 전세포(yeast whole cell)을 이용한 돼지 써코바이러스(PCV2) 서브유닛 백신과 그의 제조 방법
KR10-2013-0025823 2013-03-11
PCT/KR2014/002002 WO2014142515A1 (ko) 2013-03-11 2014-03-11 재조합 효모 전세포를 이용한 돼지 써코바이러스(pcv2) 서브유닛 백신과 그의 제조 방법

Publications (1)

Publication Number Publication Date
CN105247037A true CN105247037A (zh) 2016-01-13

Family

ID=51537083

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480026972.6A Pending CN105247037A (zh) 2013-03-11 2014-03-11 使用重组酵母全细胞的猪圆环病毒(pcv2)亚单位疫苗及其制造方法

Country Status (3)

Country Link
KR (1) KR102217387B1 (ko)
CN (1) CN105247037A (ko)
WO (1) WO2014142515A1 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104651316B (zh) * 2015-02-28 2019-03-08 上海海利生物技术股份有限公司 一种重组猪圆环病毒病毒样颗粒及其制备方法
GB2552441A (en) * 2015-10-22 2018-01-31 Royal Veterinary College Methods
KR102010061B1 (ko) * 2017-12-14 2019-08-12 주식회사 포스코 신규한 셀룰로오스 결합 도메인의 염기서열, 이를 포함하는 재조합 벡터 및 이를 활용한 단백질 분리정제 방법
CN114315983B (zh) * 2022-01-07 2023-07-25 河南兴华生物技术有限公司 猪圆环病毒亚单位疫苗

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102115755A (zh) * 2010-10-11 2011-07-06 洛阳普莱柯生物工程有限公司 酵母表达猪圆环病毒2型orf2蛋白与亚单位疫苗

Family Cites Families (3)

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FR2772047B1 (fr) * 1997-12-05 2004-04-09 Ct Nat D Etudes Veterinaires E Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection
US7276353B2 (en) * 2001-12-12 2007-10-02 Virginia Tech Intellectual Properties, Inc. Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof
KR101030792B1 (ko) * 2010-09-16 2011-04-27 주식회사 코미팜 돼지 써코바이러스２(ｐｃｖ２) 유전자의 표면발현용 벡터 및 이로 형질전환된 살모넬라 백신 균주

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102115755A (zh) * 2010-10-11 2011-07-06 洛阳普莱柯生物工程有限公司 酵母表达猪圆环病毒2型orf2蛋白与亚单位疫苗

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AAS65980.1: "capsid protein [Porcine circovirus 2]", 《GENBANK》 *
LIM等: "Recombinant Production of an Inulinase in a Saccharomyces cerevisiae gal80 Strain", 《J. MICROBIOL. BIOTECHNOL.》 *
SALLY E. ADAMS等: "The expression of hydrid HIV:Ty virus-like particles in yeast", 《NATURE》 *
SERGIO A. BUCAREY等: "The optimized capsid gene of porcine circovirus type 2 expressed in yeast forms virus-like particles and elicits antibody responses in mice fed with recombinant yeast extracts", 《VACCINE》 *

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Publication number Publication date
WO2014142515A1 (ko) 2014-09-18
KR20140111541A (ko) 2014-09-19
KR102217387B1 (ko) 2021-02-19

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Application publication date: 20160113