CN105191543B - A kind of method for breaking through Paris polyphylla seed dormancy advance germination - Google Patents
A kind of method for breaking through Paris polyphylla seed dormancy advance germination Download PDFInfo
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- CN105191543B CN105191543B CN201510742316.2A CN201510742316A CN105191543B CN 105191543 B CN105191543 B CN 105191543B CN 201510742316 A CN201510742316 A CN 201510742316A CN 105191543 B CN105191543 B CN 105191543B
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Abstract
The invention discloses a kind of method for breaking through Paris polyphylla seed dormancy advance germination, belong to Chinese medicine seed quality and cultivation field.The present invention comprises the following steps：Seeds preprocess：Full ripe Paris polyphylla seed is removed into first layer kind skin, seed is then separated into individual, selects full seed to be placed in soak at room temperature 24h 36h in mountain spring water, filters the seed floated above；Seed cleaning up：Seed after immersion is filtered dry, rinsed well；Cultivate pre-treatment：Seed after cleaning is placed in the cultivation dish equipped with Nutrition Soil or seedbed and cultivated, is sprayed water in cultivating process；Seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation.The present invention break through Paris polyphylla seed dormancy advance germination method, it is simple and easy, repeat, effect is good, effectively improves the germination percentage of Paris polyphylla seed, operating efficiency height, it is reproducible.The germination percentage of Paris polyphylla seed is 60% 70%, by cultivating up to 90% 95%.
Description
Technical field
The invention belongs to Chinese medicine seed quality and cultivation field, especially breaks through Paris polyphylla seed dormancy with a kind of ecological way
The method of advance germination is relevant.
Background technology
Paris polyphylla also known as " paris polyphylla ", it is Liliaceae Paris China Paris polyphylla Paris polylla Smith
Var.chinensis. rhizome.Paris polyphylla with the name of " flea stops " first recorded in《Sheng Nong's herbal classic》, medicinal history is long.Paris polyphylla is bitter,
It is slightly cold, slightly poisonous, the thoughts of returning home, lung, Liver Channel have the effect such as clearing heat and detoxicating, swelling and pain relieving, hemostasis, and treatment traumatic injury has uniqueness
The effect of.Paris polyphylla complex chemical composition, pharmacological activity is strong, and clinical application range is wide, and recent domestic scholar is conceived to its life
Reason activity and unique medical value, by modern pharmacological research, provide theoretical foundation, together for some clinical practices of Paris polyphylla
When be found that some new effects, it is shown that good development prospect.Paris polyphylla mainly contain a variety of saponinses such as chonglou saponin D into
Point.In recent years analyzing Paris polyphylla and its chemical composition has good antibacterial action, antiinflammatory action, anti-ulcer effect, antitumor work
With physiologically actives such as, Cardiovascular, sedation and analgesia effect, function of relieving cough and calming asthma.Paris polyphylla common are Paris polyphylla, Yunnan weight
Building, narrow leaf Paris polyphylla, gross weight building, magnificent Paris polyphylla (Paris polyphylla), white flower Paris polyphylla, P.polyphylla var.minora etc. are various in style.Due to valency in recent years
Lattice are in the trend risen year by year, and wild resource is by serious destruction, and endangered, artificial growth is bred and is a problem, is come
Source is miscellaneous, plantation is chaotic.And the characteristic of the rest period of Paris polyphylla seed tool one year, because Paris polyphylla seed has the life of 2 dormancy
Characteristic is managed, bad hydraulic permeability, natural emergence rate is poor, it is difficult to meet production requirement.The germination percentage of Paris polyphylla is studied according to relevant expert is
80%-85%, the germination percentage that planting household oneself is broadcasted sowing only have 45%-60%, and seed waste is excessive, and cost is too high, natural propagation
Slowly, germination percentage is low, emergence rate is relatively low wastes germplasm resource, fetters, constrains the development of Paris polyphylla Chinese medicine.Paris polyphylla seed
Breeding is one of main modes of reproduction (i.e. generative propagation).Because Paris polyphylla grain endosperm is hard, plumular axis is ripe slow, therefore seed
Characteristic with " dormancy ", 2 winters will be undergone by, which seeding into the ground, can just break ground emergence, that is, 2 years.So Paris polyphylla seed is bred
Emphasis be how to break the rest period of Paris polyphylla seed, make seed advance germination, emergence, improve its germination percentage and emergence rate.
The content of the invention
In view of the above-mentioned problems, the purpose of the present invention aims to provide the good ecology of a kind of simple and easy to do, favorable repeatability, effect
The method that mode breaks through Paris polyphylla seed dormancy advance germination, the germination percentage and can that this method can improve Paris polyphylla seed are complete
The holding original property of medicine of Paris polyphylla, efficiency high are reproducible.
Therefore, the present invention uses following technical scheme：A kind of method for breaking through Paris polyphylla seed dormancy advance germination, its feature
It is that the method for described breakthrough Paris polyphylla seed dormancy advance germination comprises the following steps：
1), Seeds preprocess：Full ripe Paris polyphylla seed is removed into first layer kind skin, then seed is separated into individual
Body, select full seed to be placed in soak at room temperature 24h-36h in mountain spring water, filter the seed floated above；
2), Seed cleaning up：Seed after immersion is filtered dry, rinsed well；
3) pre-treatment, is cultivated：Seed after cleaning is placed in the cultivation dish equipped with Nutrition Soil or seedbed and cultivated,
Sprayed water in cultivating process；
4) seed-bearing cultivation dish, will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation.
As the supplement to above-mentioned technical proposal and perfect, present invention additionally comprises following technical characteristic.
Seed in step 2), without removing red pericarp, is broken after being filtered dry so as to reduce artificial growth to seed
It is bad.
Nutrition Soil described in step 3) includes fertile soil and decomposed fertilizer.From fertile soil as parent-soil, that is, give Paris polyphylla seed
The growing environment of an imitative wild state is provided, natural support further is provided in germination process for Paris polyphylla seed
Point, make the thick robust seedling of its root.
Decomposed fertilizer is the ecological sheep sheep manure put in a suitable place to breed described in step 3), effectively saves the medicine under Paris polyphylla wild state
Property.
Sawdust is added in Nutrition Soil described in step 3), so as to so that seed can be happy in whole germination process
Breathing.
The mass ratio of fertile soil, decomposed fertilizer and sawdust is 2: 1: 1 in Nutrition Soil described in step 3).
Spray cycle described in step 3) is by mountain spring water sprinkling monthly at least 4 times, and cultivating soil holding humidity is
65%-75%, it is maintained at certain humidity.
Raising chamber in step 4) is by building a closed room in pollution-free area, thermostatic equipment being installed in room.
Temperature adjustment described in step 4) cultivate environment be：1-40 days temperature are 0 DEG C -10 DEG C, humidity 75%, 41-110 days
17 DEG C -24 DEG C of temperature, humidity 65%, 111-151 days temperature are 0 DEG C -10 DEG C, humidity 75%, 152-212 days temperature 17
DEG C -24 DEG C, humidity 65%, in cultivating process monthly at the 15th day, the gate for opening wide raising chamber flows into by ozone
Interior allows seed freely to breathe 3-4h, continuous 5 days.
It can reach following beneficial effect using the present invention：The method that the present invention breaks through Paris polyphylla seed dormancy advance germination,
It is simple and easy, repeat, effect is good, effectively improves the germination percentage of Paris polyphylla seed, operating efficiency is high, reproducible.Paris polyphylla kind
The germination percentage of son is 60%-70%, by cultivating up to 90%-95%.
Embodiment
Embodiment 1：
1), raising chamber：An enclosed room is built in pollution-free area, installs thermostatic equipment；
2) Seeds preprocess：Full ripe Paris polyphylla seed is removed into first layer kind skin, is separated into the individual of one one,
Soak at room temperature 24h-36h in mountain spring water is placed in, filters the seed floated above.Seed disinfectant and germinator are added in water, it is dense
Spend for 35%.
3) Seed cleaning up：Seed after immersion is filtered dry moisture, rinsed well with mountain spring water.
4) pre-treatment is cultivated：Seed after cleaning is placed in equipped with being cultivated on cultivation dish humous or seedbed.
5) cultivating seeds：Seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation：1-40 days
Temperature is 0 DEG C -10 DEG C, humidity 75%, 41-110 days 17 DEG C -24 DEG C of temperature, humidity 65%, and 111-151 days temperature are 0
DEG C -10 DEG C, humidity 75%, 152-212 days 17 DEG C -24 DEG C of temperature, humidity 65%.
This method, which is cultivated, sprouts successfully, germination percentage 75%, there is mould appearance, and mouldy rate is 25%, and budding gesture is uneven,
Bud strain is tiny.
Embodiment 2：
1), raising chamber：An enclosed room is built in pollution-free area, installs thermostatic equipment；
2) Seeds preprocess：Full ripe Paris polyphylla seed is removed into first layer kind skin, is separated into the individual of one one,
Soak at room temperature 24h-36h in mountain spring water is placed in, filters the seed floated above.Seed disinfectant and germinator are added in water, it is dense
Spend for 35%.
3) Seed cleaning up：Seed after immersion is filtered dry moisture, without removing red pericarp, rinsed with mountain spring water dry
Only；
4) pre-treatment is cultivated：Seed after cleaning is placed on cultivation dish or seedbed equipped with fertile soil and sheep manure and carried out
Cultivate.
5) cultivating seeds：To put seed-bearing cultivation dish or seedbed is placed into raising chamber, raising chamber's formalin and
Potassium permanganate carries out fumigation, and concentration is advisable with not dazzling, and stifling utensil is removed after fumigating sterilization 8 minutes, is divulged information 30 minutes,
Carry out temperature adjustment cultivation：1-40 days temperature are 0 DEG C -10 DEG C, humidity 75%, 41-110 days 17 DEG C -24 DEG C of temperature, and humidity is
65%, 111-151 days temperature are 0 DEG C -10 DEG C, humidity 75%, 152-212 days 17 DEG C -24 DEG C of temperature, humidity 65%.
This method, which is cultivated, sprouts successfully, germination percentage 80%, there is mould appearance, and mouldy rate is 15%, and budding gesture is uneven,
Bud strain is sturdy, occurs either with or without the seed of germination, and it extremely plants rate as 10%.
Embodiment 3：
1), raising chamber：An enclosed room is built in pollution-free area, installs thermostatic equipment；
2) Seeds preprocess：Full ripe Paris polyphylla seed is removed into first layer kind skin, is separated into the individual of one one,
Soak at room temperature 24h-36h in mountain spring water is placed in, filters the seed floated above, class chemical agent is expedited the emergence of without addition is any；
3) Seed cleaning up：Seed after immersion is filtered dry moisture, without removing red pericarp, rinsed with mountain spring water dry
Only；
4) pre-treatment is cultivated：Seed after cleaning is placed in the cultivation dish equipped with Nutrition Soil or seedbed and cultivated.
Need to spray water in cultivating process, water spray must be irrigated for the first time, and humidity will be controlled 75%, and insertion hygrometer is at any time
Observation, at least sprays water once weekly.
Sheep manure contains organic matter 24~27%, nitrogen (N) 0.7~0.8%, phosphorus (P2O5) 0.45~0.6%, potassium in Nutrition Soil
(K2O) 0.4~0.5%.Sheep manure is more than other animal manures containing organic matter, and excrement matter is thinner, and nutriment in a fertilizer is dense.
Sawdust in Nutrition Soil must be free from the wooden of grease.
5) cultivating seeds：Seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation：1-40 days
Temperature is 0 DEG C -10 DEG C, humidity 75%, 41-110 days 17 DEG C -24 DEG C of temperature, humidity 65%, and 111-151 days temperature are 0
DEG C -10 DEG C, humidity 75%, 152-212 days 17 DEG C -24 DEG C of temperature, humidity 65%.Monthly at the 15th day in cultivating process
When, the gate for opening wide raising chamber flows into seed by ozone and freely breathes 3-4h, continuous 5 days.
This method, which is cultivated, sprouts successfully, and germination percentage 95%, no mould occurs, and budding gesture is neat, and bud strain is sturdy.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (8)
- A kind of 1. method for breaking through Paris polyphylla seed dormancy advance germination, it is characterised in that：Described breakthrough Paris polyphylla seed dormancy carries The method of preceding germination comprises the following steps：1), Seeds preprocess：Full ripe Paris polyphylla seed is removed into first layer kind skin, seed is then separated into individual, is selected Full seed is placed in soak at room temperature 24h-36h in mountain spring water, filters the seed floated above；2), Seed cleaning up：Seed after immersion is filtered dry, rinsed well；3) pre-treatment, is cultivated：Seed after cleaning is placed in the cultivation dish equipped with Nutrition Soil or seedbed and cultivated, is cultivated During spray water；4) seed-bearing cultivation dish, will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation, the environment that temperature adjustment is cultivated is： 1-40 days temperature are 0 DEG C -10 DEG C, humidity 75%, 41-110 days 17 DEG C -24 DEG C of temperature, humidity 65%, 111-151 days temperature Spend for 0 DEG C -10 DEG C, humidity 75%, 152-212 days 17 DEG C -24 DEG C of temperature, humidity 65%, monthly in cultivating process At 15 days, the gate for opening wide raising chamber flows into interior by ozone and allows seed freely to breathe 3-4h, continuous 5 days.
- 2. according to a kind of method of breakthrough Paris polyphylla seed dormancy advance germination described in claim 1, it is characterised in that：In step 2) Seed after being filtered dry without removing red pericarp.
- A kind of 3. method for breaking through Paris polyphylla seed dormancy advance germination according to claim 1, it is characterised in that：Step 3) Described in Nutrition Soil include fertile soil and decomposed fertilizer.
- A kind of 4. method for breaking through Paris polyphylla seed dormancy advance germination according to claim 3, it is characterised in that：Step 3) Described in decomposed fertilizer be the ecological sheep sheep manure put in a suitable place to breed.
- A kind of 5. method for breaking through Paris polyphylla seed dormancy advance germination according to claim 4, it is characterised in that：Step 3) Described in Nutrition Soil in add sawdust.
- A kind of 6. method for breaking through Paris polyphylla seed dormancy advance germination according to claim 5, it is characterised in that：Step 3) Described in Nutrition Soil in fertile soil, it is decomposed fertilizer and sawdust mass ratio be 2: 1: 1.
- A kind of 7. method for breaking through Paris polyphylla seed dormancy advance germination according to claim 6, it is characterised in that：Step 3) Described in spray cycle be by mountain spring water sprinkling monthly at least 4 times, it is 65%-75% to cultivate soil and keep humidity.
- A kind of 8. method for breaking through Paris polyphylla seed dormancy advance germination according to claim 1, it is characterised in that：Step 4) In raising chamber be by building a closed room in pollution-free area, thermostatic equipment is installed in room.
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