CN105191543A - Method for germination in advance by breaking through dormancy of seeds of Paris polylla Smith var. chinensis. - Google Patents
Method for germination in advance by breaking through dormancy of seeds of Paris polylla Smith var. chinensis. Download PDFInfo
- Publication number
- CN105191543A CN105191543A CN201510742316.2A CN201510742316A CN105191543A CN 105191543 A CN105191543 A CN 105191543A CN 201510742316 A CN201510742316 A CN 201510742316A CN 105191543 A CN105191543 A CN 105191543A
- Authority
- CN
- China
- Prior art keywords
- seed
- seeds
- paris polyphylla
- paris
- dormancy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The ivneniton discloses a method for germination in advance by breaking through dormancy of seeds of Paris polylla Smith var. chinensis., and belongs to the field of seed quality and cultivation of traditional Chinese medicinal materials. The method comprises steps as follows: pretreatment of seeds: the first layers of seed coats of fully-mature seeds of Paris polylla Smith var. chinensis. are removed, individual bodies of the seeds are separated, full seeds are put in mountain spring water to be immersed for 24-36 hours at the room temperature, and seeds floating on the water are filtered out; seed clearing: the immersed seeds are dried and washed clean; pretreatment before cultivation: the washed seeds are put on a culture plate or a seed bed filled with nutrient soil to be cultivated, and water is sprayed in the cultivation process; temperature-control cultivation: the culture plate or the seed bed with seeds are put in a cultivation chamber for temperature-control cultivation. According to the method for germination in advance by breaking through dormancy of seeds of Paris polylla Smith var. chinensis., the method is simple and easy to implement, the repeatability and the effect are good, the germination rate of the seeds of Paris polylla Smith var. chinensis. can be effectively increased, the working efficiency is high, and the germination rate of the seeds of Paris polylla Smith var. chinensis. can be 60%-70% and can reach 90%-95% through cultivation.
Description
Technical field
The invention belongs to traditional Chinese medicine seed quality and cultivation field, a kind of method especially breaking through Paris polyphylla seed dormancy advance germination with ecological way is relevant.
Background technology
Paris polyphylla has another name called " paris polyphylla ", is the rhizome of Liliaceae Paris China Paris polyphylla ParispolyllaSmithvar.chinensis..Paris polyphylla is loaded in Shennong's Herbal with the name head of " flea stops ", and medicinal history is long.Paris polyphylla is bitter, is slightly cold, slightly poisonous, the thoughts of returning home, lung, Liver Channel, and have the effects such as clearing heat and detoxicating, swelling and pain relieving, hemostasis, treatment traumatic injury has unique curative effect.Paris polyphylla complex chemical composition, pharmacologically active is strong, clinical application range is wide, recent domestic scholar is conceived to its physiologically active and unique medical value, pass through modern pharmacological research, for some clinical practices of Paris polyphylla provide theoretical foundation, found some new effects to show good development prospect simultaneously.Paris polyphylla is mainly containing multiple saponin components such as chonglou saponin D.In recent years analysis Paris polyphylla and chemical composition thereof have the physiologically actives such as good antibacterial action, antiinflammatory action, anti-ulcer effect, antitumor action, Cardiovascular, sedation and analgesia effect, function of relieving cough and calming asthma.Paris polyphylla common are Paris polyphylla, Yunnan Paris polyphylla, narrow leaf Paris polyphylla, gross weight building, magnificent Paris polyphylla (Paris polyphylla), it is various in style to spend Paris polyphylla, P.polyphylla var.minora etc. in vain.Because price is in the trend risen year by year in recent years, wild resource suffers serious destruction, endangered, and artificial planting is bred and is a difficult problem, and source is assorted, plantation is chaotic.And the characteristic of Paris polyphylla seed tool resting stage one year, because Paris polyphylla seed has the physiological property of 2 dormancy, bad hydraulic permeability, natural emergence rate is poor, is difficult to meet Production requirement.The germination rate studying Paris polyphylla according to relevant expert is 80%-85%, and the germination rate that plantation family oneself is broadcasted sowing only has 45%-60%, and seed waste is excessive, cost is too high, natural propagation is slow, and germination rate is low, emergence rate is also lower wastes germplasm resource, fetters, constrains the development of Paris polyphylla traditional Chinese medicine.The breeding of Paris polyphylla seed is one of main modes of reproduction (i.e. sexual propagation).Because Paris polyphylla grain endosperm is hard, plumular axis is ripe slow, and therefore seed has the characteristic of " dormancy ", seeds into the ground will experience 2 winters and just can break ground to emerge, namely 2 years.So the emphasis of Paris polyphylla seed breeding is the resting stage of how to break Paris polyphylla seed, makes seed advance germination, emerge, improve its germination rate and emergence rate.
Summary of the invention
For the problems referred to above, object of the present invention aims to provide the method that a kind of simple and easy to do, favorable repeatability, effective ecological way break through Paris polyphylla seed dormancy advance germination, this method can improve the germination rate original property of medicine of can completely maintenance Paris polyphylla again of Paris polyphylla seed, efficiency is high, reproducible.
For this reason, the present invention is by the following technical solutions: a kind of method breaking through Paris polyphylla seed dormancy advance germination, is characterized in that, the method for described breakthrough Paris polyphylla seed dormancy advance germination comprises the following steps:
1), Seeds preprocess: by full ripe Paris polyphylla seed removing ground floor seed coat, then seed is separated into individuality, selects full seed to be placed in mountain spring water soak at room temperature 24h-36h, filter floating seed above;
2), Seed cleaning up: the seed after soaking is filtered dry, rinses well;
3), pre-treatment is cultivated: be placed in by the seed after cleaning on the cultivation dish or seedbed that Nutrition Soil is housed and cultivate, spray water in cultivating process;
4), seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation.
As to technique scheme supplement and perfect, the present invention also comprises following technical characteristic.
Step 2) in seed after being filtered dry without the need to removing red pericarp, thus decrease the destruction of artificial planting to seed.
Step 3) described in Nutrition Soil comprise humus soil and to become thoroughly decomposed fertilizer.Select humus soil as female soil, provide the growing environment of an imitative wild state namely to Paris polyphylla seed, in germination process, provide natural nutrient for Paris polyphylla seed further again, the thick seedling of its root is strengthened.
Step 3) described in become thoroughly decomposed the fertile ecological sheep sheep excrement for putting in a suitable place to breed, effectively save the property of medicine under Paris polyphylla wild state.
Step 3) described in Nutrition Soil in add sawdust, thus can make seed can happy breathing in whole germination process.
Step 3) described in Nutrition Soil in humus soil, become thoroughly decomposed fertilizer and sawdust mass ratio be 2: 1: 1.
Step 3) described in spray cycle for be sprayed monthly at least 4 times by mountain spring water, cultivate soil keep humidity be 65%-75%, remain on certain humidity.
Step 4) in raising chamber be by building a closed room in pollution-free area, in room install thermostatic equipment.
Step 4) described in temperature adjustment cultivate environment be: within 1-40 days, temperature is 0 DEG C-10 DEG C, humidity is 75%, 41-110 days temperature 17 DEG C-24 DEG C, humidity is 65%, 111-151 days temperature is 0 DEG C-10 DEG C, humidity is 75%, 152-212 days temperature 17 DEG C-24 DEG C, humidity is 65%, in cultivating process monthly the 15th day time, the gate opening wide raising chamber flows into indoor by ozone allows seed freely breathe 3-4h, continuous 5 days.
Use the present invention can reach following beneficial effect: the present invention breaks through the method for Paris polyphylla seed dormancy advance germination, simple, can repeat, the germination rate of effective, effective raising Paris polyphylla seed, operating efficiency is high, reproducible.The germination rate of Paris polyphylla seed is 60%-70%, can reach 90%-95% through cultivating.
Embodiment
Embodiment 1:
1), raising chamber: build an enclosed room in pollution-free area, install thermostatic equipment;
2) Seeds preprocess: by full ripe Paris polyphylla seed removing ground floor seed coat, be separated into the individuality of, be placed in mountain spring water soak at room temperature 24h-36h, filter floating seed above.Add seed disinfectant and germinator in water, concentration is 35%.
3) Seed cleaning up: the seed after soaking is filtered dry moisture, rinses well with mountain spring water.
4) pre-treatment is cultivated: be placed in be equipped with on cultivation dish humous or seedbed by the seed after cleaning and cultivate.
5) cultivating seeds: seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation: within 1-40 days, temperature is 0 DEG C-10 DEG C, humidity is 75%, 41-110 days temperature 17 DEG C-24 DEG C, humidity is 65%, 111-151 days temperature is 0 DEG C-10 DEG C, and humidity is 75%, 152-212 days temperature 17 DEG C-24 DEG C, humidity is 65%.
This method is cultivated and is sprouted successfully, and germination rate is 75%, has mould to occur, mouldy rate is 25%, and gesture of sprouting is uneven, and bud strain is tiny.
Embodiment 2:
1), raising chamber: build an enclosed room in pollution-free area, install thermostatic equipment;
2) Seeds preprocess: by full ripe Paris polyphylla seed removing ground floor seed coat, be separated into the individuality of, be placed in mountain spring water soak at room temperature 24h-36h, filter floating seed above.Add seed disinfectant and germinator in water, concentration is 35%.
3) Seed cleaning up: the seed after soaking is filtered dry moisture, red pericarp need not be removed, rinse well with mountain spring water;
4) pre-treatment is cultivated: be placed in by the seed after cleaning on the cultivation dish or seedbed that humus soil and sheep excrement are housed and cultivate.
5) cultivating seeds: seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, raising chamber's formalin and potassium permanganate carry out fumigation, and concentration is advisable with not dazzling, stifling utensil is removed in stifling sterilization after 8 minutes, ventilate 30 minutes, carry out temperature adjustment cultivation: within 1-40 days, temperature is 0 DEG C-10 DEG C, and humidity is 75%, 41-110 days temperature 17 DEG C-24 DEG C, humidity is 65%, 111-151 days temperature is 0 DEG C-10 DEG C, and humidity is 75%, 152-212 days temperature 17 DEG C-24 DEG C, humidity is 65%.
This method is cultivated and is sprouted successfully, and germination rate is 80%, has mould to occur, and mouldy rate is 15%, and gesture of sprouting is uneven, and bud strain is sturdy, the appearance of the seed that whether germinates, its extremely the rate of planting be 10%.
Embodiment 3:
1), raising chamber: build an enclosed room in pollution-free area, install thermostatic equipment;
2) Seeds preprocess: by full ripe Paris polyphylla seed removing ground floor seed coat, be separated into the individuality of, be placed in mountain spring water soak at room temperature 24h-36h, filter floating seed above, need not add and anyly expedite the emergence of class chemical agent;
3) Seed cleaning up: the seed after soaking is filtered dry moisture, red pericarp need not be removed, rinse well with mountain spring water;
4) pre-treatment is cultivated: be placed in by the seed after cleaning on the cultivation dish or seedbed that Nutrition Soil is housed and cultivate.
Need water spray in cultivating process, first time sprays water and must irrigate, and humidity will control 75%, inserts moisture meter and observes at any time, at least spray water once weekly.
In Nutrition Soil, sheep excrement is containing organic 24 ~ 27%, nitrogen (N) 0.7 ~ 0.8%, phosphorus (P2O5) 0.45 ~ 0.6%, potassium (K2O) 0.4 ~ 0.5%.Sheep excrement is containing organic more than other animal manure, and excrement matter is comparatively thin, and nutriment in a fertilizer is dense.
Sawdust in Nutrition Soil must be not grease-contained wooden.
5) cultivating seeds: seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation: within 1-40 days, temperature is 0 DEG C-10 DEG C, humidity is 75%, 41-110 days temperature 17 DEG C-24 DEG C, humidity is 65%, 111-151 days temperature is 0 DEG C-10 DEG C, and humidity is 75%, 152-212 days temperature 17 DEG C-24 DEG C, humidity is 65%.In cultivating process monthly the 15th day time, the gate opening wide raising chamber flows into seed by ozone and freely breathes 3-4h, continuous 5 days.
This method is cultivated and is sprouted successfully, and germination rate is 95%, occurs without mould, and gesture of sprouting is neat, and bud strain is sturdy.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (9)
1. break through a method for Paris polyphylla seed dormancy advance germination, it is characterized in that: the method for described breakthrough Paris polyphylla seed dormancy advance germination comprises the following steps:
1), Seeds preprocess: by full ripe Paris polyphylla seed removing ground floor seed coat, then seed is separated into individuality, selects full seed to be placed in mountain spring water soak at room temperature 24h-36h, filter floating seed above;
2), Seed cleaning up: the seed after soaking is filtered dry, rinses well;
3), pre-treatment is cultivated: be placed in by the seed after cleaning on the cultivation dish or seedbed that Nutrition Soil is housed and cultivate, spray water in cultivating process;
4), seed-bearing cultivation dish will be put or seedbed is placed into raising chamber, carry out temperature adjustment cultivation.
2. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 1, is characterized in that: step 2) in seed after being filtered dry without the need to removing red pericarp.
3. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 1, is characterized in that: step 3) described in Nutrition Soil comprise humus soil and to become thoroughly decomposed fertilizer.
4. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 3, is characterized in that: step 3) described in become thoroughly decomposed the fertile ecological sheep sheep excrement for putting in a suitable place to breed.
5. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 4, is characterized in that: step 3) described in Nutrition Soil in add sawdust.
6. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 5, is characterized in that: step 3) described in Nutrition Soil in humus soil, become thoroughly decomposed fertilizer and sawdust mass ratio be 2: 1: 1.
7. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 6, is characterized in that: step 3) described in spray cycle for be sprayed monthly at least 4 times by mountain spring water, cultivate soil keep humidity be 65%-75%.
8. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 1, is characterized in that: step 4) in raising chamber be by building a closed room in pollution-free area, in room install thermostatic equipment.
9. a kind of method breaking through Paris polyphylla seed dormancy advance germination according to claim 1, it is characterized in that: step 4) described in temperature adjustment cultivate environment be: within 1-40 days, temperature is 0 DEG C-10 DEG C, humidity is 75%, 41-110 days temperature 17 DEG C-24 DEG C, humidity is 65%, within 111-151 days, temperature is 0 DEG C-10 DEG C, humidity is 75%, 152-212 days temperature 17 DEG C-24 DEG C, humidity is 65%, in cultivating process monthly the 15th day time, the gate opening wide raising chamber flows into indoor by ozone allows seed freely breathe 3-4h, continuous 5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510742316.2A CN105191543B (en) | 2015-11-05 | 2015-11-05 | A kind of method for breaking through Paris polyphylla seed dormancy advance germination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510742316.2A CN105191543B (en) | 2015-11-05 | 2015-11-05 | A kind of method for breaking through Paris polyphylla seed dormancy advance germination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105191543A true CN105191543A (en) | 2015-12-30 |
| CN105191543B CN105191543B (en) | 2018-01-16 |
Family
ID=54939836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510742316.2A Active CN105191543B (en) | 2015-11-05 | 2015-11-05 | A kind of method for breaking through Paris polyphylla seed dormancy advance germination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105191543B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613649A (en) * | 2016-11-30 | 2017-05-10 | 永仁金宏农业科技开发有限公司 | Cherry seedling culture method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101189943A (en) * | 2006-11-18 | 2008-06-04 | 怀化医学高等专科学校 | Seed propagating fast breeding method for paris polyphylla |
| CN103931502A (en) * | 2014-04-29 | 2014-07-23 | 中南民族大学 | Two-step seedling growing method for propagating paris polyphylla seeds |
| CN104206148A (en) * | 2014-08-22 | 2014-12-17 | 迪庆州迪姆生态高新产业股份有限公司 | Alternate cooling and heating paris polyphylla seed propagation method |
| CN104429516A (en) * | 2014-11-24 | 2015-03-25 | 界首市聚丰家庭农场 | Ecological and standardized planting method for paris polyphylla |
-
2015
- 2015-11-05 CN CN201510742316.2A patent/CN105191543B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101189943A (en) * | 2006-11-18 | 2008-06-04 | 怀化医学高等专科学校 | Seed propagating fast breeding method for paris polyphylla |
| CN103931502A (en) * | 2014-04-29 | 2014-07-23 | 中南民族大学 | Two-step seedling growing method for propagating paris polyphylla seeds |
| CN104206148A (en) * | 2014-08-22 | 2014-12-17 | 迪庆州迪姆生态高新产业股份有限公司 | Alternate cooling and heating paris polyphylla seed propagation method |
| CN104429516A (en) * | 2014-11-24 | 2015-03-25 | 界首市聚丰家庭农场 | Ecological and standardized planting method for paris polyphylla |
Non-Patent Citations (1)
| Title |
|---|
| 李运昌: "重楼属植物引种栽培的研究", 《云南植物研究》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613649A (en) * | 2016-11-30 | 2017-05-10 | 永仁金宏农业科技开发有限公司 | Cherry seedling culture method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105191543B (en) | 2018-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104737670B (en) | A kind of method for culturing seedlings of oil seed of Paeonia suffruticosa | |
| CN105917902A (en) | Breeding method for beautiful millettia root | |
| CN103493677A (en) | Method of cutting propagation of peony immature stem | |
| CN104094762A (en) | Quick cultivation method of Paris polyphylla seedlings | |
| CN103583108A (en) | Pseudo-ginseng seedling growing method | |
| CN107455259B (en) | Cultivation method of beautiful millettia root | |
| CN105359781A (en) | Method for pepper planting | |
| CN105732214A (en) | Special mothproof fertilizer for Wogan planting and preparation method thereof | |
| CN106069137A (en) | The implantation methods of plantation Herba Anoectochili roxburghii tissue cultured seedling in a kind of booth | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| CN106986691A (en) | A kind of cultural method of spun gold emperor chrysanthemum | |
| CN107258294A (en) | A kind of breeding method of Yunnan Paris polyphylla seed | |
| CN106376419A (en) | Organic planting method of fresh peaches | |
| CN104642000B (en) | Manglietia lucida seed germinating and seedling method | |
| CN105453752A (en) | Method for promoting rapid germination of rhizoma paridis seeds | |
| CN107324887A (en) | The mating system and pawpaw seedling dedicated fertilizer of a kind of pawpaw seed | |
| CN106332757A (en) | Dendrobe seedling culture technology | |
| CN109757327B (en) | Lily planting method | |
| CN111685006A (en) | Yield-increasing and quality-improving method for preventing and treating continuous cropping obstacle of radix codonopsis pilosulae | |
| CN109526298B (en) | Passion fruit seed treatment method and sowing and breeding method thereof | |
| CN106818434A (en) | A kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing | |
| CN106717305A (en) | A kind of method for preventing and treating Achlya spp disease | |
| CN106171883A (en) | A kind of method of Fructus Lycii nursery | |
| CN105191543B (en) | A kind of method for breaking through Paris polyphylla seed dormancy advance germination | |
| CN105746275A (en) | Planting method of grapefruits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |