CN105165685A - Method for culturing double-white-shell crassostrea sikamea strain - Google Patents
Method for culturing double-white-shell crassostrea sikamea strain Download PDFInfo
- Publication number
- CN105165685A CN105165685A CN201510670668.1A CN201510670668A CN105165685A CN 105165685 A CN105165685 A CN 105165685A CN 201510670668 A CN201510670668 A CN 201510670668A CN 105165685 A CN105165685 A CN 105165685A
- Authority
- CN
- China
- Prior art keywords
- shell
- white
- oyster
- parent
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for culturing a double-white-shell crassostrea sikamea strain. Hereditary characteristics of a left white shell and a right white shell of crassostrea sikamea are different, the right white shell has an invisible hereditary character which can be stably inherited, and the left white shell has a dominant hereditary character and requires screening of homozygous dominant individual. The new double-white-shell crassostrea sikamea strain with a stable inheritance character can be obtained by the technical means of core group construction, double-white-shell individual heterozygosity testing, double-white-shell homozygous family screening, double-white-shell strain directional purification and the like in combination with the negative correlation principle of the content of melanin in a mantle and a white shell color according to a shell color hereditary mechanism. The double-white-shell crassostrea sikamea strain obtained with the method has the advantages of stable inheritance, high commercial value and the like and has an appearance as white as polished jade, an approach is provided for cultivation of the new crassostrea sikamea strain, and a new fine breed is provided for the cultivation industry of the crassostrea sikamea.
Description
Technical field:
The invention belongs to shellfish genetic breeding field in marine biotechnology, be specifically related to a kind of producing method for seed of double blank shell Kumamoto oyster strain.
Background technology:
Kumamoto oyster Crassostreasikamea originates in Southeast Asia, comprises the ground such as China, Korea S, Japan.It likes living in inclined salt water marine site in brackish water region, is distributed on the south China the Changjiang river and (comprises Nantong), as Zhejiang, Fujian, Guangdong, Guangxi, Hainan etc.Although Kumamoto oyster individuality is less, delicious flavour, in the U.S., its single live body price is higher, is long oyster, American oyster about 2 times.In China, Kumamoto oyster is known as again oyster oyster, yellow oyster, iron fourth oyster etc., and its fresh meat price is 1.5-2.0 times of Hong Kong oyster, the 2-3 of long oyster doubly, the 3-4 of Portuguese oyster doubly, is that during the huge oyster of China belongs to, head is minimum, but has a seed oyster of business development potentiality most.
Kumamoto oyster shell look has more much higher state property, the radiation type of strip such as the background colors such as frequent appearance is white, yellow, orange, purple, black and twice are black, twice are grey, twice are yellow, two spoken parts in an opera, also have two type superpositions individual, this haves laid a good foundation for we carry out Kumamoto oyster shell look strain development.
Summary of the invention:
In order to solve the problem not having new quality variety in current Kumamoto oyster culture industry, the invention provides a kind of producing method for seed of double blank shell Kumamoto oyster strain, not only can cultivate according to method of the present invention the white shell chromaticity system that outward appearance is beautiful, shell mould is neat, and provide opportunity for its rearing new variety.
The applicant finds, Kumamoto oyster left and right shell shell look genetic mechanism is different, and right shell has the phenomenon of genetic stability, and left shell often there will be heterozygosis, isozygotys, superdominance expresses genetic mechanism.For this reason, it is different that applicant utilizes Kumamoto oyster to do left and right shell color genetic mechanism, test cross technology is utilized to carry out double blank shell homozygous individual directed screening, in conjunction with melanin content negative correlation principle in white shell look and outer embrane, cultivate genetic stability, the double blank shell Kumamoto oyster strain that shell is as white as polished jade, thus achieve object of the present invention.
The producing method for seed of double blank shell Kumamoto oyster strain of the present invention, is characterized in that, comprise the following steps:
A, key foundation group build: using wild Kumamoto oyster as parent, carry out first filial generation from numerous, form according to oyster monomer conventional cultivation pattern;
The individual heterozygosity test of b, double blank shell: be the white shell Kumamoto oyster (the white shell look area of left and right shell is 100%) of white with bivalve in the first filial generation in step a and left shell is that the Kumamoto oyster of black is for material, set up n hybridization family, wherein n >=100; Meanwhile, set up n self-fertilization family (sff), wherein n >=50 that bivalve is the white shell Kumamoto oyster of white, form according to oyster monomer conventional cultivation pattern;
C, double blank shell isozygoty Genealogy screening: observe and hybridize in family in step b, whether occur left shell white, black point from, every its white shell Kumamoto oyster parent of family occurring to be separated is heterozygous parent, abandons the family that this parent participates in and makes; Otherwise in hybridization family progeny, left shell is white white shell Kumamoto oyster parent and is homozygous parent, selects this parent to carry out selfing and sets up homozygous double blank shell self-fertilization family (sff);
The directed purifying of d, double blank shell new lines: with the homozygous double blank shell self-fertilization family (sff) filial generation in step c for material, according to melanin content in outer embrane and white shell look negative correlation principle, the 10% Kumamoto oyster that in screening outer embrane, melanin content is minimum is individual as parent, carry out filial generation from numerous, namely obtain the double blank shell Kumamoto oyster strain of genetic stability.Form according to oyster monomer conventional cultivation pattern afterwards.
In described step a using wild Kumamoto oyster as parent, its parent's quantity is preferably more than 3000.
The present invention is different by the left and right shell white hereditary capacity of Kumamoto oyster, right shell white is stealthy proterties (Absorbable organic halogens heredity), left shell white is dominant character (screening homozygous dominant is individual), according to its shell look genetic mechanism, in conjunction with melanin content in outer embrane and white shell look negative correlation principle, built by core population, the individual heterozygosity test of double blank shell, double blank shell isozygoty the technological means such as Genealogy screening, the directed purifying of double blank shell strain, obtain the double blank shell Kumamoto oyster new lines of genetic stability.The double blank shell Kumamoto oyster new lines that the present invention obtains, has genetic stability, white appearance as beautiful, commodity value advantages of higher.The present invention is that the cultivation of Kumamoto new oyster variety provides approach, also for Kumamoto oyster culture industry provides new breeding.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1
A, key foundation group build: in late May, 2013, in marine economic animal experiment station, Zhanjiang of Chinese Academy of Science Nanhai Ocean Research Institute, group based on wild Kumamoto oyster 6230 individuality of Zhanjiang of maturation, colony is adopted to carry out key foundation group structure from numerous mode by the stimulation by running water that dries in the shade, amount to and produce D shape larva 1.2 hundred million, be positioned over 3 20m respectively
3in cement pit, cultivate according to the South China coastal salinity gradual change type Kumamoto oyster breeding method, when it occurs eyespot, foot overhanging stage, adopt norepinephrine process eyebot larvae, sinking device is utilized to carry out monomer cultivation, when it grows to 10-20mm, (bivalve is white to filter out double blank shell, the white shell look area of left and right shell is 100%) individual 873 of Kumamoto oyster, the individual filial generation 768 of two black shell (bivalve is black) Kumamoto oyster, adopts the mode of hanging culture to carry out monomer at island in Guangdong Province, East Sea Island, Zhanjiang water channel afterwards and forms;
B, the individual heterozygosity test of double blank shell: in late May, 2014, double blank shell Kumamoto oyster is individual, the all sexual maturity of two black shell Kumamoto oyster individuality, (90 is female to select 120 double blank shell Kumamoto oyster individualities, 30 is male), (30 is female for male individual 36 of two black shell Kumamoto oyster, 6 is male) be material, set up 120 hybridization family (male black shell=90,6 group of 15 female white shell × 1 orthogonal familys, male white shell=30, a 30 group of 1 female black shell × 1 reciprocal cross family), build double blank shell from numerous family 90 (male white shell=90,30 group of 3 female white shell × 1 self-fertilization family (sff)) simultaneously, conveniently monomer cultural technique is formed at island in Guangdong Province, East Sea Island, Zhanjiang water channel afterwards,
C, double blank shell isozygotys Genealogy screening: in late May, 2015, observe reciprocal cross family shell look genetic development, find in orthogonal family, 54 left shells of family progeny 100% are had to be white, 18 left shells of family progeny 100% are had for white in reciprocal cross family, prove in double blank shell parent, have 54 female for homozygous individual, 18 male is homozygous individual, utilizing these parents to build family of isozygotying is male white shell=54,18 group of 3 female white shell × 1 self-fertilization family (sff), find that these 54 family progeny are double blank shell individual simultaneously, ratio reaches 100%, also double blank shell parent is demonstrated for dominant homogeneous individuality, its filial generation 100% genetic stability.
D, double blank shell strain purifying: with 54 homozygous double blank shell look self-fertilization family (sff)s filial generation in step c for material, randomly draw 1620 individualities as parent (each family extracts 30 individualities), Elisa oyster melanin kit is utilized to detect melanin content in its outer embrane, according to melanin content in outer embrane and white shell look negative correlation principle, 139 of screening melanin content minimum 10% effectively individual (because the effective amount detection of parent is 1390 individualities, other 230 sample unsuccessfully) as parent, female is 112, male is 27, adopt colony from numerous mode second filial from numerous, obtain 1,640 ten thousand D shape larvas, 2400000 monomer juvenile mollusks, as its growth 10-20mm, bivalve is white, as white as polished jade, regularity reaches 100%, obtain double blank shell Kumamoto oyster strain thus.Afterwards, conveniently monomer breeding way hanging culture is formed at island in Guangdong Province, Zhanjiang water channel.
This double blank shell Kumamoto oyster strain follow-up finds through some Dai Zifan, and its filial generation is all the Kumamoto oyster that bivalve is 100% white, and can confirm thus, the invention described above obtains genetic stability, and bivalve is the double blank shell Kumamoto oyster strain of 100% white.
Above are only the specific embodiment of invention, but design concept of the present invention is not limited thereto, all changes utilizing design the present invention to be carried out to unsubstantiality, all should belong to the behavior of invading scope.
Claims (2)
1. a producing method for seed for double blank shell Kumamoto oyster strain, is characterized in that, comprise the following steps:
A, key foundation group build: using wild Kumamoto oyster as parent, carry out first filial generation from numerous, form according to oyster monomer conventional cultivation pattern;
The individual heterozygosity test of b, double blank shell: be Kumamoto oyster that the white shell Kumamoto oyster of white and left shell are black for material with bivalve in the first filial generation in step a, set up n and hybridize family, wherein n >=100; Meanwhile, set up n self-fertilization family (sff), wherein n >=50 that bivalve is the white shell Kumamoto oyster of white, form according to oyster monomer conventional cultivation pattern;
C, double blank shell isozygoty Genealogy screening: observe and hybridize in family in step b, whether occur left shell white, black point from, every its white shell Kumamoto oyster parent of family occurring to be separated is heterozygous parent, abandons the family that this parent participates in and makes; Otherwise in hybridization family progeny, left shell is white white shell Kumamoto oyster parent and is homozygous parent, selects this parent to carry out selfing and sets up homozygous double blank shell self-fertilization family (sff);
The directed purifying of d, double blank shell new lines: with the homozygous double blank shell self-fertilization family (sff) filial generation in step c for material, the 10% Kumamoto oyster that in screening outer embrane, melanin content is minimum is individual carries out filial generation from numerous as parent, namely obtains double blank shell Kumamoto oyster strain.
2. the producing method for seed of double blank shell Kumamoto oyster strain according to claim 1, is characterized in that, in described step a using wild Kumamoto oyster as parent, its parent's quantity is more than 3000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510670668.1A CN105165685B (en) | 2015-10-13 | 2015-10-13 | A kind of producing method for seed of double blank shell Kumamoto oyster strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510670668.1A CN105165685B (en) | 2015-10-13 | 2015-10-13 | A kind of producing method for seed of double blank shell Kumamoto oyster strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105165685A true CN105165685A (en) | 2015-12-23 |
| CN105165685B CN105165685B (en) | 2017-11-07 |
Family
ID=54888572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510670668.1A Active CN105165685B (en) | 2015-10-13 | 2015-10-13 | A kind of producing method for seed of double blank shell Kumamoto oyster strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105165685B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN107926790A (en) * | 2017-12-08 | 2018-04-20 | 重庆金宇恒科技有限公司 | A kind of producing method for seed for the Perna viridis strain that encrusts |
| CN107950448A (en) * | 2017-12-07 | 2018-04-24 | 重庆凌丰科技有限公司 | A kind of producing method for seed of double purple black long oyster strains of shell |
| CN111034662A (en) * | 2019-12-31 | 2020-04-21 | 广西壮族自治区水产科学研究院 | Method for obtaining hong Kong oyster monomer by drug induction |
| CN113826568A (en) * | 2020-06-24 | 2021-12-24 | 中国海洋大学 | Genetic improvement method for growth speed of crassostrea gigas orange shell color mutation strain |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN103416336A (en) * | 2013-08-16 | 2013-12-04 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| KR20130141887A (en) * | 2012-06-18 | 2013-12-27 | 최창오 | Method for culturing pearls having pattern |
| CN103858803A (en) * | 2014-03-28 | 2014-06-18 | 中国海洋大学 | Method for breeding new golden-shell crassostrea gigas strain |
-
2015
- 2015-10-13 CN CN201510670668.1A patent/CN105165685B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130141887A (en) * | 2012-06-18 | 2013-12-27 | 최창오 | Method for culturing pearls having pattern |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN103416336A (en) * | 2013-08-16 | 2013-12-04 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| CN103858803A (en) * | 2014-03-28 | 2014-06-18 | 中国海洋大学 | Method for breeding new golden-shell crassostrea gigas strain |
Non-Patent Citations (1)
| Title |
|---|
| 丛日浩: ""长牡蛎4中壳色家系子代的表型性状比较"", 《中国水产科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN106614164B (en) * | 2016-12-26 | 2019-04-30 | 中国海洋大学 | A kind of breeding method of the long oyster new lines of pure white shell |
| CN107950448A (en) * | 2017-12-07 | 2018-04-24 | 重庆凌丰科技有限公司 | A kind of producing method for seed of double purple black long oyster strains of shell |
| CN107926790A (en) * | 2017-12-08 | 2018-04-20 | 重庆金宇恒科技有限公司 | A kind of producing method for seed for the Perna viridis strain that encrusts |
| CN111034662A (en) * | 2019-12-31 | 2020-04-21 | 广西壮族自治区水产科学研究院 | Method for obtaining hong Kong oyster monomer by drug induction |
| CN113826568A (en) * | 2020-06-24 | 2021-12-24 | 中国海洋大学 | Genetic improvement method for growth speed of crassostrea gigas orange shell color mutation strain |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105165685B (en) | 2017-11-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105165685A (en) | Method for culturing double-white-shell crassostrea sikamea strain | |
| Ghose | Fisheries and aquaculture in Bangladesh: Challenges and opportunities | |
| CN103416336B (en) | Breeding method for new strain of crassostrea gigas with orange left and right shells | |
| Nair et al. | Current status and prospects of farming the giant river prawn M acrobrachium rosenbergii (D e M an) and the monsoon river prawn M acrobrachium malcolmsonii (HM E dwards) in I ndia | |
| CN102499130A (en) | Breeding method of low-salt-resistant line of blue crab | |
| CN103858803B (en) | The selection of the long oyster new lines of a kind of shell gold | |
| CN105165686B (en) | A kind of producing method for seed of double black shell Kumamoto oyster strains | |
| CN101990854A (en) | Breeding method of pure color breeding line of pacific oyster shells | |
| CN103478028A (en) | Directional cultivation method of white or purple fresh water pearl | |
| CN101617640A (en) | Seed production method of crassostrea gigas mantle pure breeding strains | |
| CN102067821A (en) | Method for breeding high-ammonia-nitrogen and high pH resistant species of prawn | |
| CN103975879A (en) | Cultivation method of new strain of quickly-grown Portuguese oysters with golden yellow shells | |
| CN101720691A (en) | Seed production method for breeding pinctada martensii | |
| CN104067962B (en) | A kind of tropical and subtropical zone aquafarm comprehensive enrichment procedure of shellfish algae | |
| CN101953321B (en) | Breeding method for high-yield new variety line of recessive white-feather gooses and application thereof | |
| CN105360022B (en) | A kind of producing method for seed of double golden shell Kumamoto oyster strains | |
| CN102422825A (en) | Method for establishing and breeding three matched breeding systems of Eriocheir sinensis | |
| CN102100201B (en) | Method for breeding ornamental type Cyprinus carpio var. color | |
| CN101861839B (en) | Method for artificially breeding purple nacre strain hyriopsis cumingii | |
| CN105360023B (en) | A kind of producing method for seed of golden adductor scar Kumamoto oyster strain | |
| Hussain et al. | Progress and the future for tilapia farming and seed production in Bangladesh | |
| CN106577424A (en) | Low-salt acclimation method of short-necked clams | |
| CN101288384A (en) | Black shrimp intraspecific hybridization method | |
| CN103478025B (en) | Cultivating method of hyriopsis cumingii with golden yellow shell | |
| CN103947588B (en) | A kind of is the Kumamoto oyster producing method for seed marked with left shell radiation ridge number |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |