CN106577424A - Low-salt acclimation method of short-necked clams - Google Patents
Low-salt acclimation method of short-necked clams Download PDFInfo
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- CN106577424A CN106577424A CN201611179413.6A CN201611179413A CN106577424A CN 106577424 A CN106577424 A CN 106577424A CN 201611179413 A CN201611179413 A CN 201611179413A CN 106577424 A CN106577424 A CN 106577424A
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- China
- Prior art keywords
- salinity
- ruditapes philippinarum
- short
- salinity gradient
- seawater
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a low-salt acclimation method of short-necked clams. The method is characterized by being implemented according to the following steps that the short-necked clams are naturally cultured in seawater with salinity being 32; the current salinity of the seawater is decreased to next salinity gradient values at intervals of 48 hours, the salinity gradient values include 30, 28, 26, 24, 22, 20, 18, 16 and 15, and still standing is performed for 6 hours after the salinity is decreased to the next salinity gradient values; and then feeding is performed according to 0.5% of the weight of the short-necked clams, when the salinity reaches 15 among the salinity gradient values, normal culturing is performed, and finally, acclimation is finished. In the acclimation process, people need to guarantee that the ammonia nitrogen content of the culturing seawater is smaller than 0.2 mg/L, the dissolved oxygen content is larger than 5 mg/L, the temperature is limited in the range of 18-20 DEG C, and the pH value is 8.2.
Description
Technical field
The present invention relates to a kind of marine product acclimation method, the less salt acclimation method of particularly a kind of Ruditapes philippinarum.
Background technology
Ruditapes philippinarum is the main marine shellfish of China, and Zhuanghe, Liaoning Province, Pulandian one are Philippine of China with coastal area
The young main producing region of clam and important export base, its production form are mudflat aquaculture.But in recent years because of coastal engineering, environmental pollution
Etc. reason, Ruditapes philippinarum mudflat aquaculture large area atrophy, industry development are faced adverse conditions.Therefore the batch production people of Ruditapes philippinarum
Work aquaculture becomes a kind of important way for obtaining Ruditapes philippinarum.During artificial cultivation is carried out, due to Philippine
The restriction of the young salinity toleration of clam, needs to allow the salinity of breeding seawater to be at least maintained at 30 or so, and if can work out
A kind of raising method of the Ruditapes philippinarum to salinity toleration, then can widen its culturing area, increase economic benefit.
The content of the invention
The present invention is, in order to solve the above-mentioned deficiency existing for prior art, to propose that one kind can effectively improve philippine clam
Son is for the less salt acclimation method of salinity toleration.
The present invention technical solution be：A kind of less salt acclimation method of Ruditapes philippinarum, it is characterised in that：Described
Method is followed the steps below：Ruditapes philippinarum is cultivated in the sea water that salinity is 32 naturally, every 48h by sea water by work as
Front salinity decrease is 30,28,26,24,22,20,18,16,15 to next salinity gradient value, described salinity gradient value, is reduced
6h is stood to next salinity gradient value, then according to the 0.5% of Ruditapes philippinarum body weight carries out bait throwing in, when salinity gradient value reaches
During to 15, normally raised, domestication is completed, in above process, it is ensured that the ammonia-nitrogen content of breeding seawater is less than 0.2mg/
L.Dissolved oxygen is more than 5mg/L, and temperature is required to in breeding seawater in pH8.2, and the overall process tamed in the range of 18-20 DEG C
Inflation.
The 6h after first bait throwing in, throws in the bait of Ruditapes philippinarum body weight 0.2% again.
The present invention compared with the existing technology, has the advantage that：
Less salt domestication is carried out to Ruditapes philippinarum using the method disclosed in the present, Ruditapes philippinarum can be significantly improved low
Survival rate under salt environment, the Ruditapes philippinarum Jing after this method domestication, its salinity tolerance limit can reduce multiple salinity units,
The median lethal time in salinity rapid drawdown can also be extended simultaneously, its appearance allows Ruditapes philippinarum advised under low-salt environment greatly
Mould artificial breeding be possibly realized, and greatly extends the cultivation scope of Ruditapes philippinarum, can produce higher economic benefit.
Description of the drawings
Fig. 1 is salinity to different lines Ruditapes philippinarum superoxide dismutase（SOD）The impact of activity.A, b, c in figure
Alphabetical different expression there is significant difference between salinity different lines of the same race（P ＜ 0.05）；X, y, z letter is different to be represented
There is significant difference between strain different salinity of the same race（P ＜ 0.05）.
Specific embodiment
The specific embodiment of the present invention is described below.As shown in Figure 1：
A kind of less salt acclimation method of Ruditapes philippinarum, follows the steps below：
First Ruditapes philippinarum is cultivated in the sea water that salinity is 32 naturally, is supported after determining, at interval of 48h by sea water by Current Salt
Angle value is reduced to next salinity gradient value, and described salinity gradient value is 30,28,26,24,22,20,18,16,15, is dropped every time
After as little as next salinity gradient value, bait throwing in will be carried out after 6h is stood, daily ration, feeding quantity is the 0.5% of Ruditapes philippinarum body weight, while
Depending on facility situation, if necessary to supplement bait throwing in, then, after first bait throwing in terminates 6h, bait is thrown in in cultivating pool again, throw
Bait amount is philippine clam the 0.2% of body weight；After salinity gradient is down to 15, you can normally raised, work is now tamed complete
Into；
During above-mentioned domestication, need to ensure that the ammonia-nitrogen content in breeding seawater is less than 0.2mg/L.Dissolved oxygen is more than 5mg/L, temperature
Degree in the range of 18-20 DEG C, pH8.2.
Embodiment
Choose the Ruditapes philippinarum of different lines：Zebra clam, orange clam, white clam and wild clam, set final salinity gradient
Value is respectively 5,15,30 3 gradients, carries out less salt domestication according to the method described above respectively, after domestication terminates, randomly selects respectively
Clam in each strain is young individual, gathers its tissue samples, makes tissue homogenate, and determine the work of superoxide dismutase
Property, it is noted that before sampling, 24h stops feeding；
As shown in Figure 1, with the reduction of salinity, the activity of the superoxide dismutase of the Ruditapes philippinarum of all strains, with
The reduction for salinity is all in rising trend, illustrates less salt for certain threat that the normal physiological of body is produced, so as to activate
Superoxide dismutase.
It is embodied in：When salinity is 15, the SOD activity of wild clam reaches highest, when salinity is 5, wild clam
Declining occurs in enzymatic activity, and considers, the final final salt angle value for determining that salinity 15 is the domestication of this method less salt.
Claims (2)
1. the less salt acclimation method of a kind of Ruditapes philippinarum, it is characterised in that：Described method is followed the steps below：By phenanthrene
Rule guest clam son is cultivated in the sea water that salinity is 32 naturally, and sea water is reduced to next salinity gradient by current salinity every 48h
Value, described salinity gradient value is 30,28,26,24,22,20,18,16,15, stands 6h after being reduced to next salinity gradient value,
Then according to the 0.5% of Ruditapes philippinarum body weight carries out bait throwing in, when salinity gradient value reaches 15, normally raised, tamed
Into in above process, it is ensured that the ammonia-nitrogen content of breeding seawater is less than 0.2mg/L；
Dissolved oxygen is more than 5mg/L, and temperature is required to breeding seawater in pH8.2, and the overall process tamed in the range of 18-20 DEG C
Middle inflation.
2. the less salt acclimation method of Ruditapes philippinarum according to claim 1, it is characterised in that：The 6h after first bait throwing in,
The bait of Ruditapes philippinarum body weight 0.2% is thrown in again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611179413.6A CN106577424A (en) | 2016-12-19 | 2016-12-19 | Low-salt acclimation method of short-necked clams |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611179413.6A CN106577424A (en) | 2016-12-19 | 2016-12-19 | Low-salt acclimation method of short-necked clams |
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| CN106577424A true CN106577424A (en) | 2017-04-26 |
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| CN201611179413.6A Pending CN106577424A (en) | 2016-12-19 | 2016-12-19 | Low-salt acclimation method of short-necked clams |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593419A (en) * | 2017-10-31 | 2018-01-19 | 广西中医药大学 | A kind of cyclic culture method of Low-salinity sea grape |
| CN112956434A (en) * | 2021-03-26 | 2021-06-15 | 青岛海洋科技馆(青岛海产博物馆、青岛水族馆) | Jellyfish germ plasm desalination culture method |
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| CN103688886A (en) * | 2013-12-16 | 2014-04-02 | 大连海洋大学 | Method for ruditapes philippinarums aquiculture in shallow sea of subtidal zone |
| CN105010764A (en) * | 2015-07-16 | 2015-11-04 | 大连海洋大学 | Low-salt tolerance scophthalmus maxima feed and scophthalmus maxima domesticating method using the low-salt tolerance feed |
| CN105379651A (en) * | 2015-11-18 | 2016-03-09 | 中国海洋大学 | Method for salinity domestication of lateolabraxjaponicus fry |
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2016
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101209031A (en) * | 2006-12-28 | 2008-07-02 | 浙江山下湖珍珠集团股份有限公司 | Desalination cultivation method for pinctada martensii |
| CN103688886A (en) * | 2013-12-16 | 2014-04-02 | 大连海洋大学 | Method for ruditapes philippinarums aquiculture in shallow sea of subtidal zone |
| CN105010764A (en) * | 2015-07-16 | 2015-11-04 | 大连海洋大学 | Low-salt tolerance scophthalmus maxima feed and scophthalmus maxima domesticating method using the low-salt tolerance feed |
| CN105379651A (en) * | 2015-11-18 | 2016-03-09 | 中国海洋大学 | Method for salinity domestication of lateolabraxjaponicus fry |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593419A (en) * | 2017-10-31 | 2018-01-19 | 广西中医药大学 | A kind of cyclic culture method of Low-salinity sea grape |
| CN112956434A (en) * | 2021-03-26 | 2021-06-15 | 青岛海洋科技馆(青岛海产博物馆、青岛水族馆) | Jellyfish germ plasm desalination culture method |
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Application publication date: 20170426 |