CN101965815A - Fine breeding method for large yellow croaker - Google Patents
Fine breeding method for large yellow croaker Download PDFInfo
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- CN101965815A CN101965815A CN2010105362975A CN201010536297A CN101965815A CN 101965815 A CN101965815 A CN 101965815A CN 2010105362975 A CN2010105362975 A CN 2010105362975A CN 201010536297 A CN201010536297 A CN 201010536297A CN 101965815 A CN101965815 A CN 101965815A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a fine breeding method for large yellow croaker, relates to a breeding method for aquatic animals, and provides the fine breeding method for the large yellow croaker, which can effectively control germplasm resources and protect the benefits of breeding units. The method comprises the following steps of: collecting the parents of the large yellow croaker which grow rapidly and have long bodies and constructing basic populations; measuring the genetic relationship of the individual parent of the large yellow croaker by microsatellite technology and establishing full-sib families of one male mating with two females by artificial insemination; breeding indoors and then transferring family fries to a sea fish raft for breeding, randomly extracting 50 to 100 fries from each family when the fries are 15 to 45g, marking with PIT, putting into a net cage for mixed breeding, measuring the characters of each family, such as growth, survival and the like every 5 to 7 months, evaluating growth speed and calculating the breeding value of each family and each individual; continuously and separately breeding the remaining individuals of each family after marking; and selecting families with high breeding value as core families from the mixedly bred populations and selecting two families with high breeding value from standby families for hybridization and seed production among the families.
Description
Technical field
The present invention relates to the breeding method of aquatic livestock, especially relate to a kind of method of in selecting breeding process, effectively carrying out family selection and individual choice by family structure, PIT mark and BLUP breeding value estimation technique.
Background technology
BLUP BLUP (Best linear unbiased prediction), it is a kind of estimation of breeding value method that grows up the middle and later periods eighties 20th century, become at present universally recognized, the advanced hereditary assessment method of Chinese scholars ([1] Sun Shouyong, 2008. the summary of animal model Blup method and application thereof [J]. animal and veterinary magazine 27,56-62; [2] Gall, G.A.E., Bakar, Y.2002.Application of mixed-model techniques to fish breed improvement:Analysis of breeding-value selection to increase 98-day body weight in tilapia.Aquaculture, 212:93-113; [3] Kong Jie, Luan Sheng, when opening the sky etc., the BLUP of 3 kinds of breeding patterns of 2008. Chinese prawns heredity assessment analysis [J]. marine fishery research 29,27-34; [4] Luan Sheng, Kong Jie opens diligent. and selection index method, Chinese patent ZL200610070100.7 are formulated in the Chinese prawn multicharacter breeding; [5] Sonessona, A.K., Gjerdea, B., Meuwissen, T.H.E., 2005.Truncation selection for BLUP-EBV and phenotypic values in fish breeding schemes[J] .Aquaculture 243,61-68.).With respect to other estimation of breeding value method, have tangible advantage based on the breeding method of BLUP: seed selection is to carry out at breeding value rather than phenotype, peeled off environmental effect, thereby efficiency of selection is higher; By design mating strategy, controlled the problem of inbreeding effectively; And this method can be controlled germ plasm resource effectively, thereby has ensured the breeding enterprise and the producer's interests.Carry out the heredity evaluation by BLUP 3 kinds of breeding patterns ([3] Kong Jie is arranged, Luan Sheng, when opening the sky etc., 2008. the BLUP of 3 kinds of breeding patterns of Chinese prawn heredity assessment analysis [J]. marine fishery research 29,27-34): meticulous breeding pattern, full sibs breeding pattern and group's breeding pattern, utilize different pedigree information (all pedigrees, full sibs, group) respectively.Comparatively speaking, meticulous breeding pattern can be utilized individual relatives' information more fully, and the genetic parameter of estimation and breeding value can be more accurate.
By the meticulous breeding technique of BLUP, external much good aquatic products kinds that selected, as Atlantic salmon, Tilapia mossambica and Penaeus Vannmei ([2] Gall, G.A.E., Bakar, Y.2002.Application of mixed-model techniques to fish breed improvement:Analysis of breeding-value selection to increase 98-day body weight in tilapia.Aquaculture, 212:93-113; [3] Kong Jie, Luan Sheng, when opening the sky etc., the BLUP of 3 kinds of breeding patterns of 2008. Chinese prawns heredity assessment analysis [J]. marine fishery research 29,27-34).
Domestic aquatic livestock based on BLUP selects breeding research to start late, and mainly concentrates on projects such as Chinese prawn, Macrobrachium rosenbergii, turbot and scallop at present.Wherein, by " No. 2, the Huanghai Sea " Chinese prawn of Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science's cultivation, " extra large Da Jin shellfish " Patinopecten yessoensis that Chinese Marine University cultivates, the new varieties such as " No. 2, lake, South Pacific " Macrobrachium rosenbergii that Zhejiang Province's fresh water is cultivated all is that utilization BLUP breeding technique is cultivated ([6] national aquatic products technology popularization master station successfully, 2010.2009 new variety of aquatic products is promoted guide. Chinese agriculture publishing house, Beijing).Domestic other many breeding units have also been carried out the research of BLUP breeding technique at present, have formed the trend of the meticulous breeding of research BLUP gradually.
Large yellow Crocker (Pseudosciaena crocea) belongs to Perciformes, Sciaenidae, yellow croaker belongs to, mainly be distributed in CHINESE OFFSHORE from southern Yellow Sea to the Central South China Sea marine site, be that China also is the important marine fish in Fujian Province ([7] Fujian Province science and technology bureau, 2004. large yellow Crocker is cultured. Maritime Press, Beijing).Since artificial breeding technology in 1987 was achieved success, large yellow Crocker was cultured the developing history that has experienced year surplus in the of 20.Yet the large yellow Crocker of culturing all is to be bred by wild population at present, does not form kind as yet.Use all not satisfactory individualities such as specification and quality to produce offspring through continuing the artificial propagation in many generations, particularly part seed rearing unit, directly cause the seed quality to descend, the growth rate of large yellow Crocker, premunition and resistance all decrease.For improving this situation, high-quality, degeneration-resistant large yellow Crocker new varieties are set about cultivating by the Department of Science and Technology, the how tame R﹠D institution of tissue of Fujian Province Science and Technology Department.At present, cultivate a plurality of strains such as " excellent No. 1 of Fujian ", " yellow ", and on producing, tentatively promoted the use of, demonstrate ([8] Zhao Guangtai of seed selection effect preferably, Liu Xiande, Wang Zhi is brave etc., 2010. large yellow Crocker continuous 4 generation breeding population genetic diversity and little satellite analysis of genetic structure. aquatic product journal 34,672-677).But the seed selection of above-mentioned strain also is based on the breeding pattern of the comparatively extensive style of Phenotypic Selection, and be difficult to the control inbreeding, if adopt the meticulous breeding pattern of selecting based on breeding value of BLUP, not only can improve the accuracy of seed selection, and can control germ plasm resource effectively, this continuation to the productivity of existing kind promotes, and is very necessary.
Summary of the invention
The object of the present invention is to provide and a kind ofly can effectively control germ plasm resource, the method for the meticulous breeding of large yellow Crocker of protection breeding units interests.
The present invention includes following steps:
1) collects the large yellow Crocker parent population that growth is fast, build is long partially, basis of formation colony;
2) utilize little satellite technology, measure the affiliation of large yellow Crocker parent population individuality,, utilize test-tube method, set up 1 hero and join 2 female family full-sibses according to the big as far as possible principle of mating both sides hereditary difference;
In step 2) in, described principle is a Nei formula genetic distance greater than 1 (referring to document [9] Nei evolutionary-genetics.Columbia University Press M.1987.Molecular, New York), described family full-sibs can adopt 30～100.
3) behind the indoor culture family fry being transferred to marine fishing row cultures, the independent net cage of each family, when supporting body weight 15～45g, each family is randomly drawed 50～100 tails, carry out mixed breed with being put into net cage behind the PIT mark, measured the growth of each family every 5～7 months, proterties such as survival, the assessment growth rate, use MTDFREML software (, 23:67-83) to calculate each family and breeding value that each is individual referring to document [11] Meyer variances and covariances for multivariate animal models by restricted maximum likelihood.Genet.Sel.Evol. K.1991.Estimating; Remaining individuality continues separately to culture (reserve family) behind each family mark, further makes productivity and relatively confirms;
In step 3), the time of described indoor culture can be 35～40 days; The specification of the independent net cage of described each family can be length and width and is 4.2m, dark 4m; Described with PIT mark (passive integration radar mark) can referring to document [10] ([10] Cai Mingyi, Chen Jianlin, Liu Xiande etc., the assessment of 2008.PIT label large yellow Crocker method. " mariculture research new development " Maritime Press, Beijing, 104-109); Describedly can be length and width and be 8.4m, dark 6m with being put into specification that net cage carries out the net cage of mixed breed behind the PIT mark.
4) select and remain the high family of breeding value as core families from mixed breed colony, enter next circulation, 2 the high familys of breeding value of selecting and remain from 30～100 reserve familys are carried out the tame line cross production of hybrid seeds.
In step 4), the high family of the described breeding value of selecting and remain from mixed breed colony is as core families, and the high family of 15 breeding values of can selecting and remain from mixed breed colony is as core families.
Compared with the prior art the present invention has following outstanding advantage:
1) seed selection is to select at breeding value rather than phenotypic value, has improved the accuracy and the efficient of seed selection.
2),, controlled inbreeding effectively according to the far and near design of affiliation mating strategy by little satellite analysis.
3) owing to can effectively control germ plasm resource, the breeding enterprise and the producer's interests have therefore been ensured.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and need not limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, unspecified technology is a routine techniques.
Meticulous breeding method of the present invention comprises basic population method for building up, family construction method, genetic parameter and breeding value method of estimation and mating strategy method for designing.
(1) the large yellow Crocker parent population basis of formation colony that growth is fast, build is grown is partially collected in high mud sea area in Ningde, Fujian Jiaocheng District bight fishing row, Xiapu Xia Hu steck, Liaanjiang county Xia Yu fishing row, the Ningbo of Zhejiang Xiangshan.
(2) utilize little satellite technology, measure the affiliation of parent population individuality, little satellite analysis uses primer as shown in table 1.According to little satellite analysis result, according to the big as far as possible principle of mating both sides hereditary difference (Nei formula genetic distance is greater than 1), utilize test-tube method, set up 1 hero and join 30 of 2 female family full-sibses;
(3) the family fry is transferred to marine fishing row and cultured, the independent net cage of each family (cage size: length and width is 4.2m, dark 4m).Fry is plunged into the commercial sea when supporting body weight 15～45g, each family is randomly drawed 100 tails, (cage size: length and width is 8.4m with being put into a big net cage behind the PIT mark (passive integration radar mark), dark 6m) carries out mixed breed, measure the proterties such as growth, survival of each family every half a year, the assessment growth rate.Utilize MTDFREML software to calculate each family and individual breeding value (table 2); Remaining individuality continues separately to culture (reserve individuality) behind each family mark, further makes productivity and relatively confirms.
(4), in the middle of 15 high familys of breeding value, select 600 tails and make up core population (individuality of aggregate breeding value rank before 40 each family) according to the height of family breeding value.
(5) seed produces.According to each family aggregate breeding value size, in conjunction with the affiliation between family, the excellent individual of 2 superior families of selecting and remain from 30 reserve familys is carried out the tame line cross production of hybrid seeds, produces seed 3,000,000 tail supply the markets.
Table 1. is used for the micro-satellite primers that the analysis of Different Individual large yellow Crocker genetic distance is used
The long breeding value estimated result of the individual 13 monthly age bodies of table 2 large yellow Crocker part
Claims (7)
1. the method for the meticulous breeding of large yellow Crocker is characterized in that may further comprise the steps:
1) collects the large yellow Crocker parent population that growth is fast, build is long partially, basis of formation colony;
2) utilize little satellite technology, measure the affiliation of large yellow Crocker parent population individuality,, utilize test-tube method, set up 1 hero and join 2 female family full-sibses according to the big as far as possible principle of mating both sides hereditary difference;
3) behind the indoor culture family fry being transferred to marine fishing row cultures, the independent net cage of each family, when supporting body weight 15～45g, each family is randomly drawed 50～100 tails, carry out mixed breed with being put into net cage behind the PIT mark, measured the proterties such as growth, survival of each family every 5～7 months, the assessment growth rate uses MTDFREML software to calculate each family and breeding value that each is individual; Remaining individuality continues separately to culture behind each family mark, further makes productivity and relatively confirms;
4) select and remain the high family of breeding value as core families from mixed breed colony, enter next circulation, 2 the high familys of breeding value of selecting and remain from 30～100 reserve familys are carried out the tame line cross production of hybrid seeds.
2. the method for the meticulous breeding of a kind of large yellow Crocker as claimed in claim 1 is characterized in that in step 2) in, described principle is that Nei formula genetic distance is greater than 1.
3. the method for the meticulous breeding of a kind of large yellow Crocker as claimed in claim 1 is characterized in that in step 2) in, described family full-sibs is 30～100.
4. the method for the meticulous breeding of a kind of large yellow Crocker as claimed in claim 1 is characterized in that in step 3), and the time of described indoor culture is 35～40 days.
5. the method for the meticulous breeding of a kind of large yellow Crocker as claimed in claim 1 is characterized in that in step 3), and the specification of the independent net cage of described each family is that length and width is 4.2m, dark 4m.
6. the method for the meticulous breeding of a kind of large yellow Crocker as claimed in claim 1 is characterized in that in step 3), and described is that length and width is 8.4m with being put into the specification that net cage carries out the net cage of mixed breed behind the PIT mark, dark 6m.
7. the method for the meticulous breeding of a kind of large yellow Crocker as claimed in claim 1, it is characterized in that in step 4), the high family of the described breeding value of selecting and remain from mixed breed colony is as core families, is to select and remain the high family of 15 breeding values as core families from mixed breed colony.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124978A (en) * | 2011-04-10 | 2011-07-20 | 浙江海洋学院 | Large yellow croaker breeding method |
| CN102144592A (en) * | 2011-04-12 | 2011-08-10 | 中国水产科学研究院淡水渔业研究中心 | Rapid and efficient method for breeding tilapias |
| CN102613119A (en) * | 2012-04-11 | 2012-08-01 | 宁德市富发水产有限公司 | Method for transferring larimichthys crocea fries quickly |
| CN103882144A (en) * | 2014-04-14 | 2014-06-25 | 中国水产科学研究院黄海水产研究所 | Method for evaluating breeding value of turbot family |
| CN108728551A (en) * | 2018-04-09 | 2018-11-02 | 中国长江三峡集团公司中华鲟研究所 | A kind of breeding method keeping mandarin sturgeon merit |
| CN114657262A (en) * | 2022-03-31 | 2022-06-24 | 集美大学 | Molecular marker assisted breeding method for anti-visceral white spot large yellow croaker |
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| CN1957684A (en) * | 2006-11-14 | 2007-05-09 | 中国水产科学研究院黄海水产研究所 | Method for establishing selection index for breeding Chinese prawn in multiple characters |
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| CN1736184A (en) * | 2005-08-10 | 2006-02-22 | 宁波大学 | An artificial cultured pseudosciaena crocea and culturing method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102124978A (en) * | 2011-04-10 | 2011-07-20 | 浙江海洋学院 | Large yellow croaker breeding method |
| CN102124978B (en) * | 2011-04-10 | 2012-10-03 | 浙江海洋学院 | Large yellow croaker breeding method |
| CN102144592A (en) * | 2011-04-12 | 2011-08-10 | 中国水产科学研究院淡水渔业研究中心 | Rapid and efficient method for breeding tilapias |
| CN102613119A (en) * | 2012-04-11 | 2012-08-01 | 宁德市富发水产有限公司 | Method for transferring larimichthys crocea fries quickly |
| CN103882144A (en) * | 2014-04-14 | 2014-06-25 | 中国水产科学研究院黄海水产研究所 | Method for evaluating breeding value of turbot family |
| CN103882144B (en) * | 2014-04-14 | 2016-01-20 | 中国水产科学研究院黄海水产研究所 | A kind of turbot family breeding value appraisal procedure |
| CN108728551A (en) * | 2018-04-09 | 2018-11-02 | 中国长江三峡集团公司中华鲟研究所 | A kind of breeding method keeping mandarin sturgeon merit |
| CN108728551B (en) * | 2018-04-09 | 2022-02-08 | 中国长江三峡集团公司中华鲟研究所 | Breeding method for keeping fine characters of Chinese sturgeons |
| CN114657262A (en) * | 2022-03-31 | 2022-06-24 | 集美大学 | Molecular marker assisted breeding method for anti-visceral white spot large yellow croaker |
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