CN105360023B - A kind of producing method for seed of golden adductor scar Kumamoto oyster strain - Google Patents
A kind of producing method for seed of golden adductor scar Kumamoto oyster strain Download PDFInfo
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- CN105360023B CN105360023B CN201510670772.0A CN201510670772A CN105360023B CN 105360023 B CN105360023 B CN 105360023B CN 201510670772 A CN201510670772 A CN 201510670772A CN 105360023 B CN105360023 B CN 105360023B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of producing method for seed of golden adductor scar Kumamoto oyster strain.Present invention firstly discovers that ratio extremely low golden adductor scar Kumamoto oyster, there is the characteristic of epistatic effect according to Kumamoto oyster gold adductor scar, with reference to the quantitative criteria of golden colourity, by technological means such as parental line selection, colourity assessment, filial generation cultivation, strain purifying, so as to cultivate golden adductor scar Kumamoto oyster strain.The golden adductor scar Kumamoto oyster strain of the present invention, have the advantages that stable heredity, commodity value are high.The present invention provides approach for Kumamoto oyster rearing new variety, and new breeding is also provided for Kumamoto oyster aquaculture.
Description
Technical field：
The invention belongs to shellfish genetic breeding field in marine biotechnology, and in particular to a kind of golden adductor scar Kumamoto
The producing method for seed of oyster strain.
Background technology：
Kumamoto oyster Crassostreasikamea originates in Southeast Asia, including the ground such as China, South Korea, Japan, Vietnam.It
It is the Important Economic kind in China's the South China coastal cultivated shellfish, likes the brackish water lived in nearly river mouth or nearby have fresh water to inject
Middle high salt marine site in region, is mainly distributed on the south the Changjiang river (except Nantong), including Zhejiang, Guangdong, Guangxi, Hainan etc.
Ground.In the past, it is outer successively to cultivate different shell colors, difference according to oyster shell color and its outer embrane retrochromism by scholars
The oyster new lines (Wang Qingzhi, 2009) of mantle color.Although some scholars are found that oyster adductor scar, there is color change
Change, but relevant report is black, white and its middle transition color.
The content of the invention：
In order to solve the problems, such as do not have high-quality new lines (kind) in current Kumamoto oyster aquaculture, the invention provides one kind
The producing method for seed of golden adductor scar Kumamoto oyster strain, possibility is provided for its rearing new variety.
Found by the research of inventor team in recent years：There is extremely low ratio gold adductor scar in Kumamoto oyster
Body, and the color individual can stablize heredity, this provides possibility for the research and development of golden adductor scar oyster new lines.
The producing method for seed of the golden adductor scar Kumamoto oyster strain of the present invention, it is characterised in that comprise the following steps：
A, parental line selection：The group based on wild Kumamoto oyster, using anatomy method, filter out golden adductor scar
Kumamoto oyster, then the golden colourity of golden adductor scar is assessed with chromascope, closed with gold of the golden colourity in 60-80
The Kumamoto oyster of shell flesh trace is as target parent；
B, filial generation is cultivated：By the use of target parent as parent, filial generation is carried out from numerous, is supported using conventional monomeric cultural method
Into, until sexal maturity, detect the adductor scar color of first filial generation sexal maturity adult, with golden colourity 60-80 golden closed shell
The Kumamoto oyster of flesh trace is as target parent；
C, strain purifies：2-3 progress strain purifying of repeat step b is (i.e. using adductor scar gold colourity 60-80 as mark
Standard, purified by 2-3 for strain), that is, the bear of golden adductor scar of hereditary (more than 95%) can be stablized by obtaining golden colourity
This oyster strain.
In described parental line selection, parent's quantitative requirement is more than 10000, it is ensured that the golden closed shell flesh that preliminary screening arrives
Trace individual amount prevents inbreeding depression occur from numerous filial generation more than 100；
It is that alternatively colony, guarantee determine parent's adductor scar color with sexal maturity group in described parental line selection
After can produce seed in time.
It is that chromaticity requirements can not be excessively light using adult Kumamoto oyster as object is assessed during described colourity is assessed, but
It is unable to overrich, it is ensured that be beautiful golden yellow；
During described filial generation is cultivated, filial generation quantitative requirement is more than 10,000, it is ensured that statistics accuracy rate；
In described strain purifying, gold be can be stably it is hereditary, the golden colourity of main purification, make it appear to
Golden yellow chroma color is consistent.
The present invention carries out oyster new lines cultivation first using adductor scar color as Kumamoto oyster criterion for breeding.This hair
It is bright to be different from documents 1 (Zeng Zhinan, Ning Yue, Qi Jianfei, Wu Qisheng, a kind of golden yellow fast lady's fingers tooth oyster new product of shell
The breeding method of system, national inventing patent, the patent No.：201410197671.1) and documents 2 (Li Qi, Kong Lingfeng, Wang Wei
Army, Cong Hao, Ge Jianlong, Wang Xubo, a kind of selection of the long oyster new lines of shell gold, national inventing patent, application number：
201410120162.9).Compared with the present invention, documents 1 and 2 are selected using oyster shell appearance face color as standard
Select, and the present invention is with the alternatively index of adductor scar color in Kumamoto oyster shell inner surface, carries out selection, between the two
Selection standard is entirely different, illustrates that above documents not yet have any impact to originality of the present invention.
The present invention has the characteristic of epistatic effect using Kumamoto oyster gold adductor scar first, with reference to golden colourity conduct
Criterion, by technological means such as parental line selection, colourity assessment, filial generation cultivation, strain purifying, so as to cultivate golden closed shell
Flesh trace color Kumamoto oyster strain.The golden adductor scar Kumamoto oyster strain that the present invention obtains, there is stable heredity, commodity value
The advantages that high.The present invention provides approach for Kumamoto oyster rearing new variety, and new breeding is also provided for Kumamoto oyster aquaculture.
Brief description of the drawings：
Fig. 1 is different colours adductor scar Kumamoto oyster strain, respectively black adductor scar, golden yellow adductor scar,
White adductor scar individual.
Embodiment：
Following examples are to further explanation of the invention, rather than limitation of the present invention.
Embodiment 1
A, parental line selection：In the first tenday period of a month in May, 2013, in Chinese Academy of Science Nanhai Ocean Research Institute's Zhanjiang marine economic animal
Experiment station, the group based on the wild individual of Kumamoto oyster 12000 in Zhanjiang of maturation, golden closed shell flesh is carried out using anatomy method
Trace oyster parental population preliminary screening, it is found that 107 individuals are golden adductor scar；
B, colourity is assessed：In order that its adductor scar solid colour, is assessed its golden colourity using chromascope, with
Golden colourity is used as target parent flock in 75 individuals of 60-80 adductor scars；
C, filial generation is cultivated：Female individuals are 63 in 75 target parent flocks, and male is 12, is fertilized using mixing
Method carry out filial generation from numerous, seed is cultivated using salinity gradual change type Kumamoto oyster artificial breeding method, kept hanging afterwards in Zhanjiang
East Sea Island island in Guangdong Province water channel is formed, and is cultivated by the anniversary, and oyster sexual gland is ripe；
D, strain purifies：Using adductor scar gold colourity 60-80 as standard, 10000 first filial generation adult closed shell fleshes are detected
Trace color, it is found that 8750 individuals meet standard, target parent flock is used as using it；The method that target parent flock is fertilized using mixing
Filial generation is carried out from numerous, seed is cultivated using salinity gradual change type Kumamoto oyster artificial breeding method, kept hanging afterwards in Zhanjiang East Sea Island
Island in Guangdong Province water channel is formed, and is cultivated by the anniversary, and oyster sexual gland is ripe, using adductor scar gold colourity 60-80 as standard, detection
Two generations sexal maturity adult adductor scar color, target parent flock is used as using standard compliant；Target parent flock is fertilized using mixing
Method carry out filial generation from numerous, seed is cultivated using salinity gradual change type Kumamoto oyster artificial breeding method, kept hanging afterwards in Zhanjiang
East Sea Island island in Guangdong Province water channel is formed, and is cultivated by the anniversary, and oyster sexual gland is ripe, using adductor scar gold colourity 60-80 as standard,
F3 adult adductor scar color is detected, 15600 adult individuals is detected by third time, finds 14976 individual closed shell fleshes
Trace gold colourity is in 60-80.As can be seen here, orient and purify by 3 generations, 96% golden adductor scar colourity, which can be stablized, to be lost
Pass, that is, obtain the Kumamoto oyster strain of golden adductor scar.
Embodiment 2
A, parental line selection：In in early June, 2013, in Chinese Academy of Science Nanhai Ocean Research Institute's Zhanjiang marine economic animal
Experiment station, the group based on the wild individual of U.S.'s Kumamoto oyster 15000 of maturation, golden closed shell flesh is carried out using anatomy method
Trace oyster parental population preliminary screening, it is found that 163 individuals are golden adductor scar；
B, colourity is assessed：In order that its adductor scar solid colour, is assessed its golden colourity using chromascope, with
Golden colourity is used as target parent flock in 124 individuals of 60-80 adductor scars；
C, filial generation is cultivated：In 124 target parent flocks female individuals be 105, male be 19, using mixing by
The method of essence carries out filial generation from numerous, cultivates seed using salinity gradual change type Kumamoto oyster artificial breeding method, keeps hanging afterwards in Zhan
East of a river island island in Guangdong Province water channel is formed, and is cultivated by the anniversary, and oyster sexual gland is ripe；
D, strain purifies：Using adductor scar gold colourity 60-80 as standard, 10000 first filial generation adult Kumamotos of detection are male
Oyster adductor scar color, it is found that 9137 individuals meet standard, target parent flock is used as using it；Target parent flock using mixing by
The method of essence carries out filial generation from numerous, cultivates seed using salinity gradual change type Kumamoto oyster artificial breeding method, keeps hanging afterwards in Zhan
East of a river island island in Guangdong Province water channel is formed, and is cultivated by the anniversary, and oyster sexual gland is ripe, using adductor scar gold colourity 60-80 as mark
Standard, second filial adult Kumamoto oyster adductor scar color is detected, target parent flock is used as using standard compliant；Target parent mines massively
Carries out filial generation from numerous with the method for mixing fertilization, using salinity gradual change type Kumamoto oyster artificial breeding method cultivation seed, afterwards
Keep hanging and formed in Zhanjiang East Sea Island island in Guangdong Province water channel, cultivated by the anniversary, oyster sexual gland is ripe, with adductor scar gold colourity
60-80 is standard, detects F3 adult Kumamoto oyster adductor scar color, and 14500 adult individuals are detected by third time,
It was found that 14210 individual adductor scar gold colourities are in 60-80.As can be seen here, orient and purify by 3 generations, 98% gold is closed
Shell flesh trace colourity can stablize heredity, that is, obtain the Kumamoto oyster strain of golden adductor scar.
Claims (1)
1. a kind of producing method for seed of golden adductor scar Kumamoto oyster strain, it is characterised in that comprise the following steps：
A, parental line selection：The group based on wild Kumamoto oyster, using anatomy method, filter out the Kumamoto of golden adductor scar
Oyster, then assessed with chromascope the golden colourity of golden adductor scar, with golden colourity 60-80 golden closed shell flesh
The Kumamoto oyster of trace is as target parent；
B, filial generation is cultivated：By the use of target parent as parent, filial generation is carried out from numerous, is formed using conventional monomeric cultural method, directly
To sexal maturity, detect the adductor scar color of first filial generation sexal maturity adult, with golden colourity 60-80 golden adductor scar
Kumamoto oyster is as target parent；
C, strain purifies：2-3 progress strain purifying of repeat step b, that is, obtain the Kumamoto oyster strain of golden adductor scar.
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| CN201510670772.0A CN105360023B (en) | 2015-10-13 | 2015-10-13 | A kind of producing method for seed of golden adductor scar Kumamoto oyster strain |
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| CN105360023B true CN105360023B (en) | 2017-12-05 |
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| JP2007053957A (en) * | 2005-08-24 | 2007-03-08 | Iyo Toyotama Shinju Kk | Method for selecting pearl oyster |
| CN101393006B (en) * | 2008-11-10 | 2010-08-11 | 淮海工学院 | Germplasm identification method for moerella irideseens |
| CN101617640A (en) * | 2009-07-30 | 2010-01-06 | 中国海洋大学 | Seed production method of crassostrea gigas mantle pure breeding strains |
| CN101990854A (en) * | 2010-10-29 | 2011-03-30 | 中国海洋大学 | Breeding method of pure color breeding line of pacific oyster shells |
| CN103858803B (en) * | 2014-03-28 | 2015-12-09 | 中国海洋大学 | The selection of the long oyster new lines of a kind of shell gold |
| CN103975879B (en) * | 2014-05-12 | 2015-08-26 | 福建省水产研究所 | The breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell |
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