CN105164253B - 葡糖淀粉酶变体和编码它们的多核苷酸 - Google Patents

葡糖淀粉酶变体和编码它们的多核苷酸

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Publication number
CN105164253B
CN105164253B CN201480024499.8A CN201480024499A CN105164253B CN 105164253 B CN105164253 B CN 105164253B CN 201480024499 A CN201480024499 A CN 201480024499A CN 105164253 B CN105164253 B CN 105164253B
Authority
CN
China
Prior art keywords
variant
glucoamylase
substitution
starch
alpha
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201480024499.8A
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English (en)
Chinese (zh)
Other versions
CN105164253A (zh
Inventor
堤纪子
绫部圭一
岸下诚一郎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
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Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Publication of CN105164253A publication Critical patent/CN105164253A/zh
Application granted granted Critical
Publication of CN105164253B publication Critical patent/CN105164253B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480024499.8A 2013-04-30 2014-04-29 葡糖淀粉酶变体和编码它们的多核苷酸 Active CN105164253B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13165995.5 2013-04-30
EP13165995 2013-04-30
PCT/EP2014/058692 WO2014177546A2 (en) 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
CN105164253A CN105164253A (zh) 2015-12-16
CN105164253B true CN105164253B (zh) 2025-11-14

Family

ID=48190353

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480024499.8A Active CN105164253B (zh) 2013-04-30 2014-04-29 葡糖淀粉酶变体和编码它们的多核苷酸

Country Status (9)

Country Link
US (1) US10550374B2 (enExample)
EP (2) EP3372680B1 (enExample)
CN (1) CN105164253B (enExample)
BR (1) BR112015026972B1 (enExample)
CA (1) CA2904022C (enExample)
DK (2) DK2992092T3 (enExample)
ES (2) ES2835313T3 (enExample)
MX (2) MX368655B (enExample)
WO (1) WO2014177546A2 (enExample)

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EP2893009A4 (en) * 2012-09-07 2016-03-02 Novozymes As GLYCOAMYLASE VARIANTS AND POLYNUCLEOTIDE CODING THEREWITH, AND THEIR USE
RU2706297C2 (ru) * 2014-02-07 2019-11-15 Новозимс А/С Композиции для получения глюкозных сиропов
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US10308963B2 (en) 2015-02-27 2019-06-04 Novozymes A/S Processes of producing ethanol using a fermenting organism
WO2016205127A1 (en) 2015-06-18 2016-12-22 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
US10494685B2 (en) 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
EP3610027A1 (en) 2017-04-11 2020-02-19 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
CA3064042A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
WO2019005755A1 (en) 2017-06-28 2019-01-03 Novozymes A/S POLYPEPTIDES HAVING TREHALASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
EP3665275A1 (en) 2017-08-08 2020-06-17 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
MX2020007914A (es) 2018-01-29 2020-10-28 Novozymes As Microorganismos con uso de nitrogeno mejorado para produccion de etanol.
WO2019161227A1 (en) 2018-02-15 2019-08-22 Novozymes A/S Improved yeast for ethanol production
CA3098718A1 (en) 2018-05-31 2019-12-05 Novozymes A/S Processes for enhancing yeast growth and productivity
MX2021000893A (es) 2018-07-25 2021-03-31 Novozymes As Levadura que expresa enzimas para la producción de etanol.
MX2021003955A (es) 2018-10-08 2021-05-27 Novozymes As Levadura que expresa enzimas para la produccion de etanol.
CA3143381A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
WO2021025872A1 (en) 2019-08-06 2021-02-11 Novozymes A/S Fusion proteins for improved enzyme expression
BR112022011271A2 (pt) 2019-12-10 2022-09-06 Novozymes As Célula hospedeira recombinante, composição, métodos para produzir um derivado de uma célula e um produto de fermentação, e, uso de uma célula hospedeira recombinante
WO2022261003A1 (en) 2021-06-07 2022-12-15 Novozymes A/S Engineered microorganism for improved ethanol fermentation
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137704A2 (en) 2022-12-19 2024-06-27 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
CN120380158A (zh) 2022-12-19 2025-07-25 诺维信公司 用于降低发酵产物生产方法后端的浆体粘度的方法
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization

Citations (3)

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WO2006069289A2 (en) * 2004-12-22 2006-06-29 Novozymes North America, Inc Polypeptides having glucoamylase activity and polynucleotides encoding same
WO2011066576A1 (en) * 2009-11-30 2011-06-03 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
WO2011066560A1 (en) * 2009-11-30 2011-06-03 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same

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WO2011066576A1 (en) * 2009-11-30 2011-06-03 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
WO2011066560A1 (en) * 2009-11-30 2011-06-03 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same

Also Published As

Publication number Publication date
ES2835313T3 (es) 2021-06-22
MX368655B (es) 2019-10-10
DK3372680T3 (da) 2021-01-11
BR112015026972B1 (pt) 2023-05-02
MX2019005158A (es) 2019-08-05
BR112015026972A2 (enExample) 2017-09-05
CN105164253A (zh) 2015-12-16
EP2992092A2 (en) 2016-03-09
ES2674701T3 (es) 2018-07-03
CA2904022C (en) 2022-04-19
EP2992092B1 (en) 2018-04-04
EP3372680B1 (en) 2020-11-11
WO2014177546A3 (en) 2014-12-24
US20160152960A1 (en) 2016-06-02
CA2904022A1 (en) 2014-11-06
DK2992092T3 (en) 2018-06-25
MX2015014537A (es) 2016-02-05
EP3372680A1 (en) 2018-09-12
WO2014177546A2 (en) 2014-11-06
US10550374B2 (en) 2020-02-04

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CB03 Change of inventor or designer information

Inventor after: Dijizi

Inventor after: Thin silk fabric section Gui one

Inventor after: Kishinoshita Seiichiro

Inventor before: Didianzi

Inventor before: Satin department built one

Inventor before: S - based chihi tower

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