ES2835313T3 - Variantes de glucoamilasa y polinucleótidos que las codifican - Google Patents

Variantes de glucoamilasa y polinucleótidos que las codifican Download PDF

Info

Publication number
ES2835313T3
ES2835313T3 ES18163981T ES18163981T ES2835313T3 ES 2835313 T3 ES2835313 T3 ES 2835313T3 ES 18163981 T ES18163981 T ES 18163981T ES 18163981 T ES18163981 T ES 18163981T ES 2835313 T3 ES2835313 T3 ES 2835313T3
Authority
ES
Spain
Prior art keywords
variant
glucoamylase
polypeptide
alpha
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES18163981T
Other languages
English (en)
Spanish (es)
Inventor
Noriko Tsutsumi
Keiichi Ayabe
Seiichiro Kishishita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of ES2835313T3 publication Critical patent/ES2835313T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/20Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES18163981T 2013-04-30 2014-04-29 Variantes de glucoamilasa y polinucleótidos que las codifican Active ES2835313T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP13165995 2013-04-30

Publications (1)

Publication Number Publication Date
ES2835313T3 true ES2835313T3 (es) 2021-06-22

Family

ID=48190353

Family Applications (2)

Application Number Title Priority Date Filing Date
ES14723388.6T Active ES2674701T3 (es) 2013-04-30 2014-04-29 Variantes de glucoamilasa y polinucleótidos que las codifican
ES18163981T Active ES2835313T3 (es) 2013-04-30 2014-04-29 Variantes de glucoamilasa y polinucleótidos que las codifican

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES14723388.6T Active ES2674701T3 (es) 2013-04-30 2014-04-29 Variantes de glucoamilasa y polinucleótidos que las codifican

Country Status (9)

Country Link
US (1) US10550374B2 (enExample)
EP (2) EP3372680B1 (enExample)
CN (1) CN105164253B (enExample)
BR (1) BR112015026972B1 (enExample)
CA (1) CA2904022C (enExample)
DK (2) DK3372680T3 (enExample)
ES (2) ES2674701T3 (enExample)
MX (2) MX368655B (enExample)
WO (1) WO2014177546A2 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9879241B2 (en) 2012-09-07 2018-01-30 Novozymes A/S Glucoamylase variants and polynucleotides encoding same and uses thereof
BR112016018075B1 (pt) 2014-02-07 2022-01-18 Novozymes A/S Composição compreendendo uma alfa-amilase, uma pululanase e uma enzima glicoamilase e método de fabricação de xarope de glicose a partir de amido liquefeito
DK3209788T3 (da) 2014-10-23 2019-08-26 Novozymes As Glucoamylasevarianter og polynukleotider, som koder for dem
AU2016225049B2 (en) 2015-02-27 2022-03-31 Microbiogen Pty. Ltd. Processes of producing ethanol using a fermenting organism
WO2016205127A1 (en) 2015-06-18 2016-12-22 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
US10494685B2 (en) 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
EP3610027A1 (en) * 2017-04-11 2020-02-19 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US20200157581A1 (en) 2017-06-02 2020-05-21 Novozymes A/S Improved Yeast For Ethanol Production
WO2019005755A1 (en) 2017-06-28 2019-01-03 Novozymes A/S POLYPEPTIDES HAVING TREHALASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME
WO2019030165A1 (en) 2017-08-08 2019-02-14 Novozymes A/S POLYPEPTIDES HAVING TREHALASE ACTIVITY AND THEIR USE IN A PRODUCTION PROCESS OF FERMENTATION PRODUCTS
US20220348967A1 (en) 2018-01-29 2022-11-03 Novozymes A/S Microorganisms With Improved Nitrogen Utilization For Ethanol Production
CA3107124A1 (en) 2018-02-15 2019-08-22 Novozymes A/S Improved yeast for ethanol production
MX2020012754A (es) 2018-05-31 2021-02-26 Novozymes As Procesos para mejorar el crecimiento y la productividad de la levadura.
WO2020023411A1 (en) 2018-07-25 2020-01-30 Novozymes A/S Enzyme-expressing yeast for ethanol production
WO2020076697A1 (en) 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
CN114450390A (zh) 2019-07-26 2022-05-06 诺维信公司 用于乙醇生产的氮转运提高的微生物
CN114127124A (zh) 2019-08-06 2022-03-01 诺维信公司 用于提高酶表达的融合蛋白
AU2020402990A1 (en) 2019-12-10 2022-06-09 Novozymes A/S Microorganism for improved pentose fermentation
CN117795089A (zh) 2021-06-07 2024-03-29 诺维信公司 用于改善的乙醇发酵的工程化微生物
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
CN120380158A (zh) 2022-12-19 2025-07-25 诺维信公司 用于降低发酵产物生产方法后端的浆体粘度的方法
WO2024137250A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
WO2024137246A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
AU2024303961A1 (en) 2023-06-13 2025-12-11 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
WO1989001969A1 (en) 1987-09-04 1989-03-09 Novo-Nordisk A/S Process for the production of protein products in aspergillus and promoters for use in aspergillus
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DK81293D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
KR970703426A (ko) 1994-06-03 1997-07-03 제임스 쉐한 정제된 Myceliophthora 락카제 및 그것을 암호화 하는 핵산(PURIFIED MYCELIOPHTHORA LACCASES AND NUCLEIC ACIDS ENCODING SAME)
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
DK2199378T3 (da) 1995-02-03 2012-10-29 Novozymes As alfa-amylase mutanter
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
ES2432519T3 (es) 1996-04-30 2013-12-04 Novozymes A/S Mutantes de alfa-amilasa
ES2436066T3 (es) 1997-10-13 2013-12-26 Novozymes A/S Mutantes de alfa-amilasa
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
DK1058724T3 (en) 1998-02-27 2011-03-21 Novozymes As Maltogene alpha-amylase varianter
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
CN1526820B (zh) 1999-03-22 2010-05-26 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
CN1252256C (zh) 1999-03-30 2006-04-19 诺维信公司 α-淀粉酶变体
AU2001278415A1 (en) 2000-08-01 2002-02-13 Novozymes A/S Alpha-amylase mutants with altered stability
CA2455013A1 (en) 2001-07-27 2003-02-13 Francesca Storici Systems for in vivo site-directed mutagenesis using oligonucleotides
ES2245621T3 (es) 2003-03-10 2010-05-19 Novozymes A/S Procesos para un producto alcoholico.
EP2336308B1 (en) 2003-06-25 2014-08-13 Novozymes A/S Enzymes for starch processing
MXPA06000212A (es) 2003-06-25 2006-03-21 Novozymes As Enzimas para procesar almidon.
ES2621921T3 (es) 2004-12-22 2017-07-05 Novozymes A/S Enzimas para tratamiento de almidón
WO2007144424A2 (en) 2006-06-15 2007-12-21 Novozymes A/S Processes for production of a starch hydrolysate
ES2534066T3 (es) * 2009-11-30 2015-04-17 Novozymes A/S Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
US8916359B2 (en) * 2009-11-30 2014-12-23 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
CA2782036C (en) 2009-12-01 2019-01-15 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi

Also Published As

Publication number Publication date
WO2014177546A3 (en) 2014-12-24
BR112015026972A2 (enExample) 2017-09-05
CA2904022C (en) 2022-04-19
MX368655B (es) 2019-10-10
US20160152960A1 (en) 2016-06-02
EP2992092A2 (en) 2016-03-09
DK3372680T3 (da) 2021-01-11
CN105164253B (zh) 2025-11-14
BR112015026972B1 (pt) 2023-05-02
EP2992092B1 (en) 2018-04-04
WO2014177546A2 (en) 2014-11-06
CN105164253A (zh) 2015-12-16
DK2992092T3 (en) 2018-06-25
US10550374B2 (en) 2020-02-04
EP3372680A1 (en) 2018-09-12
ES2674701T3 (es) 2018-07-03
MX2015014537A (es) 2016-02-05
MX2019005158A (es) 2019-08-05
CA2904022A1 (en) 2014-11-06
EP3372680B1 (en) 2020-11-11

Similar Documents

Publication Publication Date Title
ES2835313T3 (es) Variantes de glucoamilasa y polinucleótidos que las codifican
US11840713B2 (en) Glucoamylase variants and polynucleotides encoding same
ES2656556T3 (es) Variantes de glucoamilasa y polinucleótidos que codifican las mismas
US9963690B2 (en) Glucoamylase variants and polynucleotides encoding same
US9695406B2 (en) Glucoamylase variants and polynucleotides encoding same
BR122022018788B1 (pt) Variantes de glucoamilase, composição, uso de um polipeptídeo, processo de produção de um produto de fermentação, processo de produção de um produto de xarope, polinucleotídeos isolado, constructo de ácido nucleico, vetor de expressão e célula hospedeira