CN105133040A - 一种检测海洋致病弧菌的基因芯片及其制备方法与检测方法 - Google Patents
一种检测海洋致病弧菌的基因芯片及其制备方法与检测方法 Download PDFInfo
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- CN105133040A CN105133040A CN201510379388.5A CN201510379388A CN105133040A CN 105133040 A CN105133040 A CN 105133040A CN 201510379388 A CN201510379388 A CN 201510379388A CN 105133040 A CN105133040 A CN 105133040A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106811542A (zh) * | 2017-03-28 | 2017-06-09 | 大连海洋大学 | 用于检测参虾贝养殖区致病弧菌群的基因芯片和使用方法 |
CN106957913A (zh) * | 2017-03-28 | 2017-07-18 | 大连海洋大学 | 一种海胆致病菌强壮弧菌的检测方法 |
CN107012227A (zh) * | 2017-04-21 | 2017-08-04 | 丹东出入境检验检疫局综合技术服务中心 | 用于致病性弧菌检测的通用引物对及其应用 |
CN107267633A (zh) * | 2017-07-20 | 2017-10-20 | 杭州更蓝生物科技有限公司 | 一种利用基因芯片检测结核分枝杆菌的方法 |
CN108707090A (zh) * | 2018-03-30 | 2018-10-26 | 宁波大学 | 一种含氯芳香化合物及其制备方法和用途 |
CN109187970A (zh) * | 2018-12-05 | 2019-01-11 | 鲁东大学 | 一种快速检测水产病害金标核酸试纸条及其制备方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101691608A (zh) * | 2009-06-03 | 2010-04-07 | 宁波大学 | 水产品养殖致病菌的基因芯片 |
WO2010060262A1 (zh) * | 2008-11-03 | 2010-06-03 | 天津生物芯片技术有限责任公司 | 检测水产品中重要致病菌的基因芯片和试剂盒 |
WO2012016375A1 (zh) * | 2010-08-03 | 2012-02-09 | 中国水产科学研究院黄海水产研究所 | 检测多种海水养殖动物病原菌的基因芯片及其应用 |
CN103451310A (zh) * | 2013-09-23 | 2013-12-18 | 国家海洋局第一海洋研究所 | 一种可并行检测多种弧菌的基因芯片及其检测方法 |
CN103540668A (zh) * | 2013-10-22 | 2014-01-29 | 宁波大学 | 检测10种海域致病菌的基因芯片 |
CN104017877A (zh) * | 2014-06-12 | 2014-09-03 | 南京农业大学 | 一种副溶血性弧菌分子检测方法及使用的引物对 |
-
2015
- 2015-06-30 CN CN201510379388.5A patent/CN105133040B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010060262A1 (zh) * | 2008-11-03 | 2010-06-03 | 天津生物芯片技术有限责任公司 | 检测水产品中重要致病菌的基因芯片和试剂盒 |
CN101691608A (zh) * | 2009-06-03 | 2010-04-07 | 宁波大学 | 水产品养殖致病菌的基因芯片 |
WO2012016375A1 (zh) * | 2010-08-03 | 2012-02-09 | 中国水产科学研究院黄海水产研究所 | 检测多种海水养殖动物病原菌的基因芯片及其应用 |
CN103451310A (zh) * | 2013-09-23 | 2013-12-18 | 国家海洋局第一海洋研究所 | 一种可并行检测多种弧菌的基因芯片及其检测方法 |
CN103540668A (zh) * | 2013-10-22 | 2014-01-29 | 宁波大学 | 检测10种海域致病菌的基因芯片 |
CN104017877A (zh) * | 2014-06-12 | 2014-09-03 | 南京农业大学 | 一种副溶血性弧菌分子检测方法及使用的引物对 |
Non-Patent Citations (3)
Title |
---|
于永翔等: "基于gyrB基因的SYBR 1 Green I 实时定量PCR 检测病原灿烂弧菌", 《渔业科学进展》 * |
杨芳等: "副溶血弧菌毒力基因和保守基因研究进展", 《预防医学情报杂志》 * |
王淑娴: "海洋弧菌中五类溶血素基因分布及其生物学活性研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106811542A (zh) * | 2017-03-28 | 2017-06-09 | 大连海洋大学 | 用于检测参虾贝养殖区致病弧菌群的基因芯片和使用方法 |
CN106957913A (zh) * | 2017-03-28 | 2017-07-18 | 大连海洋大学 | 一种海胆致病菌强壮弧菌的检测方法 |
CN106957913B (zh) * | 2017-03-28 | 2020-06-16 | 大连海洋大学 | 一种海胆致病菌强壮弧菌的检测方法 |
CN106811542B (zh) * | 2017-03-28 | 2020-08-21 | 大连海洋大学 | 用于检测参虾贝养殖区致病弧菌群的基因芯片和使用方法 |
CN107012227A (zh) * | 2017-04-21 | 2017-08-04 | 丹东出入境检验检疫局综合技术服务中心 | 用于致病性弧菌检测的通用引物对及其应用 |
CN107267633A (zh) * | 2017-07-20 | 2017-10-20 | 杭州更蓝生物科技有限公司 | 一种利用基因芯片检测结核分枝杆菌的方法 |
CN108707090A (zh) * | 2018-03-30 | 2018-10-26 | 宁波大学 | 一种含氯芳香化合物及其制备方法和用途 |
CN109187970A (zh) * | 2018-12-05 | 2019-01-11 | 鲁东大学 | 一种快速检测水产病害金标核酸试纸条及其制备方法 |
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Inventor after: Su Xiurong Inventor after: Zhou Jun Inventor after: Zhang Dijun Inventor after: Zhang Xuan Inventor after: Zhang Chundan Inventor after: He Shan Inventor after: Li Taiwu Inventor before: Zhou Jun Inventor before: Su Xiurong Inventor before: Zhang Dijun Inventor before: Zhang Xuan Inventor before: Zhang Chundan Inventor before: He Shan Inventor before: Li Taiwu |