CN1051317C - 用置换色谱纯化血红蛋白产物的方法 - Google Patents

用置换色谱纯化血红蛋白产物的方法 Download PDF

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Publication number
CN1051317C
CN1051317C CN94190708A CN94190708A CN1051317C CN 1051317 C CN1051317 C CN 1051317C CN 94190708 A CN94190708 A CN 94190708A CN 94190708 A CN94190708 A CN 94190708A CN 1051317 C CN1051317 C CN 1051317C
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CN
China
Prior art keywords
hemoglobin
column
solution
hbao
impurities
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN94190708A
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English (en)
Chinese (zh)
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CN1114836A (zh
Inventor
D·朴陆拉
D·威芬
S·阿史拉福
A·A·马格因
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Hemosol Inc
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Hemosol Inc
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Publication date
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Priority claimed from ZA951034A external-priority patent/ZA951034B/xx
Publication of CN1114836A publication Critical patent/CN1114836A/zh
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Publication of CN1051317C publication Critical patent/CN1051317C/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN94190708A 1993-09-21 1994-09-20 用置换色谱纯化血红蛋白产物的方法 Expired - Fee Related CN1051317C (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA002106612A CA2106612C (en) 1993-09-21 1993-09-21 Displacement chromatography process
CA2,106,612 1993-09-21
ZA951034A ZA951034B (en) 1993-09-21 1995-02-09 Displacements chromatography process and purified hemoglobin product

Publications (2)

Publication Number Publication Date
CN1114836A CN1114836A (zh) 1996-01-10
CN1051317C true CN1051317C (zh) 2000-04-12

Family

ID=25676656

Family Applications (1)

Application Number Title Priority Date Filing Date
CN94190708A Expired - Fee Related CN1051317C (zh) 1993-09-21 1994-09-20 用置换色谱纯化血红蛋白产物的方法

Country Status (9)

Country Link
US (1) US5439591A (enExample)
EP (1) EP0668877B1 (enExample)
CN (1) CN1051317C (enExample)
AU (1) AU681438B2 (enExample)
CA (1) CA2106612C (enExample)
CZ (1) CZ287589B6 (enExample)
PL (1) PL179525B1 (enExample)
SK (1) SK281839B6 (enExample)
WO (1) WO1995008574A1 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545328A (en) * 1993-09-21 1996-08-13 Hemosol Inc. Purification of hemoglobin by displacement chromatography
US5741894A (en) * 1995-09-22 1998-04-21 Baxter International, Inc. Preparation of pharmaceutical grade hemoglobins by heat treatment in partially oxygenated form
JP2002515880A (ja) * 1996-08-27 2002-05-28 ヘモソル インク． 赤血球造血の増強刺激
DE69831248T2 (de) 1997-02-28 2006-04-13 The Regents Of The University Of California, Oakland Verfahren und zusammensetzungen zur optimierung des sauerstofftransportes in zellfreien systemen
US5814601A (en) * 1997-02-28 1998-09-29 The Regents Of The University Of California Methods and compositions for optimization of oxygen transport by cell-free systems
US5851400A (en) * 1997-03-21 1998-12-22 University Of Maryland At Baltimore County Method for eliminating the displacer in displacement chromatography of proteins
US6423233B1 (en) * 2000-08-15 2002-07-23 Biogal Gyogyszergyar Rt. Purification process
GB9707696D0 (en) * 1997-04-16 1997-06-04 Agner Erik Improvements in or relating to chromatography
US6239262B1 (en) 1998-01-07 2001-05-29 Rensselaer Polytechnic Institute Low molecular weight displacers for protein purification in hydrophobic interaction and reversed phase chromatographic systems
CA2233725A1 (en) 1998-03-31 1999-09-30 Hemosol Inc. Hemoglobin-hydroxyethyl starch complexes
GB9822963D0 (en) * 1998-10-20 1998-12-16 Agner Erik Improvements in or relating to chromatography
US6573373B1 (en) 1999-06-29 2003-06-03 Isis Pharmaceuticals, Inc. High affinity, low molecular weight displacers for oligonucleotide purification
US6747132B2 (en) 2000-11-29 2004-06-08 Apex Biosciences, Inc. Methods for the synthesis of a modified hemoglobin solution
US7195923B2 (en) * 2001-01-31 2007-03-27 Scripps Laboratories, Inc. Ratiometric determination of glycated protein
CA2437381C (en) 2001-02-01 2012-03-27 Sigma-Aldrich Co. Improved affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
US20050164915A1 (en) 2002-04-01 2005-07-28 Sangart, Inc. Compositions for oxygen transport comprising a high oxygen affinity modified hemoglobin
US20030153491A1 (en) * 2002-01-11 2003-08-14 Winslow Robert M. Methods and compositions for oxygen transport comprising a high oxygen affinity modified hemoglobin
US6929747B2 (en) * 2002-02-28 2005-08-16 Renesselaer Polytechnic Institute High-affinity, low-molecular-mass displacers for ion-exchange chromatography
JP3912206B2 (ja) * 2002-07-05 2007-05-09 株式会社日立製作所 筒内直接燃料噴射装置用燃料ポンプ
KR101482791B1 (ko) 2005-03-11 2015-01-21 와이어쓰 엘엘씨 약한 분배성 크로마토그래피법
DE112006001012B4 (de) * 2005-04-22 2017-05-18 Waters Technologies Corp. (N.D.Ges.D. Staates Delaware) Verfahren für eine auf Massenspektrometrie gerichtete Aufbereitung von Biopolymeren
JP5001949B2 (ja) * 2005-12-02 2012-08-15 サッチェム，インコーポレイテッド 陰イオン交換置換クロマトグラフィー方法および陰イオン交換置換クロマトグラフィー方法における置換剤化合物として用いられる陰イオン性有機化合物
WO2009138992A1 (en) * 2008-05-12 2009-11-19 Secretary, Department Of Atomic Energy An innovative 'cut -and-feed' operation for enhancing the performance of ion-exchange chromatographic separation
KR101843915B1 (ko) † 2008-08-14 2018-04-02 제넨테크, 인크. 고유 단백질 대체 이온 교환 막 크로마토그래피를 이용한 오염물의 제거 방법
KR101844859B1 (ko) 2009-08-06 2018-05-18 제넨테크, 인크. 단백질 정제 시의 바이러스 제거의 개선방법
US8304248B2 (en) * 2009-11-16 2012-11-06 Torres Anthony R Protein separation via ion-exchange chromatography and associated methods, systems, and devices
MY161534A (en) 2010-05-25 2017-04-28 Genentech Inc Methods of purifying polypeptides
WO2012068134A1 (en) 2010-11-15 2012-05-24 Biogen Idec Inc. Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography
US8293115B2 (en) * 2011-01-20 2012-10-23 Gordon Rossiter Ionic impurities rejection and chromatographic purification using ion exchange
GB201103363D0 (en) * 2011-02-28 2011-04-13 Ge Healthcare Uk Ltd Sample preservation method and sample application substrate
TR201903707T4 (tr) * 2011-11-02 2019-04-22 Hoffmann La Roche Aşırı yükleme ve elüsyon kromatografisi.
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
CN103665098B (zh) * 2012-09-20 2015-08-05 中国科学院大连化学物理研究所 双相柱膜蛋白质微反应器及其应用
HUE038741T2 (hu) 2013-05-06 2018-11-28 Sanofi Sa Folyamatos többlépéses eljárás antitestek tisztítására
EP3546475A1 (en) 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
ES2982322T3 (es) * 2018-04-18 2024-10-15 Sekisui Medical Co Ltd Método de análisis de hemoglobina

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925574A (en) * 1985-06-26 1990-05-15 Canadian Patents & Development Limited Purification of hemoglobin and modified hemoglobin by affinity chromatography
US5028696A (en) * 1988-10-28 1991-07-02 Torres Anthony R Ion exchange and separation method
US5115100A (en) * 1988-08-17 1992-05-19 Kinetic Investments Limited Purification of hemoglobin and methemoglobin by bioselective elution

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3971842A (en) * 1974-08-08 1976-07-27 Atlantic Richfield Company Continuous displacement chromatographic separation
FR2548670B1 (fr) * 1983-07-07 1985-10-25 Merieux Inst Procede de preparation des principales proteines du sang hemolyse sous forme non denaturee
JPS6075530A (ja) * 1983-09-30 1985-04-27 Asahi Chem Ind Co Ltd 金属元素の新しい分離精製方法
US5084558A (en) * 1987-10-13 1992-01-28 Biopure Corporation Extra pure semi-synthetic blood substitute
US5340474A (en) * 1988-03-24 1994-08-23 Terrapin Technologies, Inc. Panels of analyte-binding ligands
US5149436A (en) * 1988-11-28 1992-09-22 Union Oil Company Of California Continuous displacement chromatographic method
IT1232311B (it) * 1989-09-04 1992-01-28 Sclavo Spa Metodo di purificazione di composti a struttura peptidica o pseudo peptidica a basso peso molecolare.
US5264555A (en) * 1992-07-14 1993-11-23 Enzon, Inc. Process for hemoglobin extraction and purification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4925574A (en) * 1985-06-26 1990-05-15 Canadian Patents & Development Limited Purification of hemoglobin and modified hemoglobin by affinity chromatography
US5115100A (en) * 1988-08-17 1992-05-19 Kinetic Investments Limited Purification of hemoglobin and methemoglobin by bioselective elution
US5028696A (en) * 1988-10-28 1991-07-02 Torres Anthony R Ion exchange and separation method

Also Published As

Publication number Publication date
SK67895A3 (enExample) 1995-09-13
WO1995008574A1 (en) 1995-03-30
CZ128795A3 (en) 1995-12-13
SK281839B6 (sk) 2001-08-06
US5439591A (en) 1995-08-08
EP0668877A1 (en) 1995-08-30
AU681438B2 (en) 1997-08-28
AU7650394A (en) 1995-04-10
PL179525B1 (pl) 2000-09-29
CZ287589B6 (en) 2000-12-13
CA2106612A1 (en) 1995-03-22
EP0668877B1 (en) 1999-01-20
CA2106612C (en) 2001-02-06
PL309034A1 (en) 1995-09-18
CN1114836A (zh) 1996-01-10

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Granted publication date: 20000412

Termination date: 20091020