CA2106612C - Displacement chromatography process - Google Patents
Displacement chromatography processInfo
- Publication number
- CA2106612C CA2106612C CA002106612A CA2106612A CA2106612C CA 2106612 C CA2106612 C CA 2106612C CA 002106612 A CA002106612 A CA 002106612A CA 2106612 A CA2106612 A CA 2106612A CA 2106612 C CA2106612 C CA 2106612C
- Authority
- CA
- Canada
- Prior art keywords
- column
- hemoglobin
- stage
- solution
- chromatographic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 71
- 230000008569 process Effects 0.000 title claims abstract description 57
- 238000002086 displacement chromatography Methods 0.000 title abstract description 9
- 108010054147 Hemoglobins Proteins 0.000 claims abstract description 114
- 102000001554 Hemoglobins Human genes 0.000 claims abstract description 114
- 239000000243 solution Substances 0.000 claims abstract description 42
- 239000003480 eluent Substances 0.000 claims abstract description 35
- 125000002091 cationic group Chemical group 0.000 claims abstract description 23
- 125000000129 anionic group Chemical group 0.000 claims abstract description 22
- 230000002378 acidificating effect Effects 0.000 claims abstract description 15
- 239000012045 crude solution Substances 0.000 claims abstract description 8
- 239000007864 aqueous solution Substances 0.000 claims abstract description 5
- 241000894007 species Species 0.000 claims description 37
- 238000004587 chromatography analysis Methods 0.000 claims description 29
- 241000700605 Viruses Species 0.000 claims description 15
- 238000006073 displacement reaction Methods 0.000 claims description 12
- 239000012527 feed solution Substances 0.000 claims description 12
- 239000003633 blood substitute Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 3
- 239000000356 contaminant Substances 0.000 abstract description 32
- 238000000926 separation method Methods 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 description 26
- 108090000623 proteins and genes Proteins 0.000 description 26
- 239000000872 buffer Substances 0.000 description 22
- 238000011068 loading method Methods 0.000 description 20
- 238000000746 purification Methods 0.000 description 17
- 239000000499 gel Substances 0.000 description 16
- 239000007983 Tris buffer Substances 0.000 description 15
- 210000003743 erythrocyte Anatomy 0.000 description 15
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 15
- 239000006166 lysate Substances 0.000 description 13
- 239000000047 product Substances 0.000 description 9
- 238000011084 recovery Methods 0.000 description 9
- 238000005349 anion exchange Methods 0.000 description 8
- 239000000427 antigen Substances 0.000 description 8
- 102000036639 antigens Human genes 0.000 description 8
- 108091007433 antigens Proteins 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 230000003612 virological effect Effects 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 7
- 239000003957 anion exchange resin Substances 0.000 description 7
- 238000013459 approach Methods 0.000 description 7
- 238000010828 elution Methods 0.000 description 7
- 238000005342 ion exchange Methods 0.000 description 7
- 238000001155 isoelectric focusing Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 239000011347 resin Substances 0.000 description 6
- 229920005989 resin Polymers 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 5
- 238000011067 equilibration Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 206010012735 Diarrhoea Diseases 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 241000580858 Simian-Human immunodeficiency virus Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 239000003656 tris buffered saline Substances 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- 241000700584 Simplexvirus Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000005571 anion exchange chromatography Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 125000003636 chemical group Chemical group 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000004255 ion exchange chromatography Methods 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 239000012160 loading buffer Substances 0.000 description 3
- 230000002934 lysing effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 238000009928 pasteurization Methods 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 241000991587 Enterovirus C Species 0.000 description 2
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- -1 agarose based) gels Chemical class 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 238000011097 chromatography purification Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000011026 diafiltration Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 208000006454 hepatitis Diseases 0.000 description 2
- 231100000283 hepatitis Toxicity 0.000 description 2
- 230000000951 immunodiffusion Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 150000003904 phospholipids Chemical class 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000013341 scale-up Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 102000003846 Carbonic anhydrases Human genes 0.000 description 1
- 108090000209 Carbonic anhydrases Proteins 0.000 description 1
- 108010044495 Fetal Hemoglobin Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 102000028180 Glycophorins Human genes 0.000 description 1
- 108091005250 Glycophorins Proteins 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 241000709701 Human poliovirus 1 Species 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000012518 Poros HS 50 resin Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000005890 Spectrin Human genes 0.000 description 1
- 108010019965 Spectrin Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000005277 cation exchange chromatography Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 238000007813 chromatographic assay Methods 0.000 description 1
- 239000012501 chromatography medium Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011035 continuous diafiltration Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000011118 depth filtration Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 108010036066 hemoglobin AA Proteins 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004237 preparative chromatography Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000012289 standard assay Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Diabetes (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (20)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002106612A CA2106612C (en) | 1993-09-21 | 1993-09-21 | Displacement chromatography process |
| US08/187,316 US5439591A (en) | 1993-09-21 | 1994-01-27 | Displacement chromatography process |
| HU9501479A HU218159B (hu) | 1993-09-21 | 1994-09-20 | Kiszorításos kromatográfiás eljárás tisztított hemoglobintermék előállítására |
| AU76503/94A AU681438B2 (en) | 1993-09-21 | 1994-09-20 | Displacement chromatography process and purified hemoglobin product |
| CN94190708A CN1051317C (zh) | 1993-09-21 | 1994-09-20 | 用置换色谱纯化血红蛋白产物的方法 |
| SK678-95A SK281839B6 (sk) | 1993-09-21 | 1994-09-20 | Spôsob separácie vopred vybraného druhu hemoglobínu |
| DE69416103T DE69416103T2 (de) | 1993-09-21 | 1994-09-20 | Verdrängungschromatographisches verfahren und gereinigtes hämoglobin |
| AT94926756T ATE175975T1 (de) | 1993-09-21 | 1994-09-20 | Verdrängungschromatographisches verfahren und gereinigtes hämoglobin |
| ES94926756T ES2126775T3 (es) | 1993-09-21 | 1994-09-20 | Procedimiento cromatografico de desplazamiento y producto de hemoglobina purificada. |
| EP94926756A EP0668877B1 (en) | 1993-09-21 | 1994-09-20 | Displacement chromatography process and purified hemoglobin product |
| UA95058439A UA35599C2 (uk) | 1993-09-21 | 1994-09-20 | Спосіб очищення гемоглобіну з сирого розчину |
| PL94309034A PL179525B1 (pl) | 1993-09-21 | 1994-09-20 | Sposób wydzielania hemoglobiny z surowego roztworu metoda chromatografii PL |
| CZ19951287A CZ287589B6 (en) | 1993-09-21 | 1994-09-20 | Process of separating a preselected hemoglobin species from a crude solution thereof |
| DK94926756T DK0668877T3 (da) | 1993-09-21 | 1994-09-20 | Fortrængningschromatografisk fremgangsmåde og oprenset hæmoglobinprodukt |
| PCT/CA1994/000514 WO1995008574A1 (en) | 1993-09-21 | 1994-09-20 | Displacement chromatography process and purified hemoglobin product |
| JP50945895A JP4057049B2 (ja) | 1993-09-21 | 1994-09-20 | 置換クロマトグラフィー法および精製ヘモグロビン産物 |
| RU95112503A RU2145873C1 (ru) | 1993-09-21 | 1994-09-20 | Способ очистки гемоглобина в коммерческом масштабе |
| KR1019950701487A KR100361894B1 (ko) | 1993-09-21 | 1994-09-21 | 치환크로마토그라피방법및정제된헤모글로빈생성물 |
| ZA951034A ZA951034B (en) | 1993-09-21 | 1995-02-09 | Displacements chromatography process and purified hemoglobin product |
| US08/406,148 US5545328A (en) | 1993-09-21 | 1995-03-20 | Purification of hemoglobin by displacement chromatography |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002106612A CA2106612C (en) | 1993-09-21 | 1993-09-21 | Displacement chromatography process |
| ZA951034A ZA951034B (en) | 1993-09-21 | 1995-02-09 | Displacements chromatography process and purified hemoglobin product |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2106612A1 CA2106612A1 (en) | 1995-03-22 |
| CA2106612C true CA2106612C (en) | 2001-02-06 |
Family
ID=25676656
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002106612A Expired - Lifetime CA2106612C (en) | 1993-09-21 | 1993-09-21 | Displacement chromatography process |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5439591A (enExample) |
| EP (1) | EP0668877B1 (enExample) |
| CN (1) | CN1051317C (enExample) |
| AU (1) | AU681438B2 (enExample) |
| CA (1) | CA2106612C (enExample) |
| CZ (1) | CZ287589B6 (enExample) |
| PL (1) | PL179525B1 (enExample) |
| SK (1) | SK281839B6 (enExample) |
| WO (1) | WO1995008574A1 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545328A (en) * | 1993-09-21 | 1996-08-13 | Hemosol Inc. | Purification of hemoglobin by displacement chromatography |
| US5741894A (en) * | 1995-09-22 | 1998-04-21 | Baxter International, Inc. | Preparation of pharmaceutical grade hemoglobins by heat treatment in partially oxygenated form |
| WO1998008537A1 (en) * | 1996-08-27 | 1998-03-05 | Hemosol Inc. | Enhanced stimulation of erythropoiesis |
| US6054427A (en) * | 1997-02-28 | 2000-04-25 | The Regents Of The University Of California | Methods and compositions for optimization of oxygen transport by cell-free systems |
| US5814601A (en) * | 1997-02-28 | 1998-09-29 | The Regents Of The University Of California | Methods and compositions for optimization of oxygen transport by cell-free systems |
| US5851400A (en) * | 1997-03-21 | 1998-12-22 | University Of Maryland At Baltimore County | Method for eliminating the displacer in displacement chromatography of proteins |
| US6423233B1 (en) * | 2000-08-15 | 2002-07-23 | Biogal Gyogyszergyar Rt. | Purification process |
| GB9707696D0 (en) * | 1997-04-16 | 1997-06-04 | Agner Erik | Improvements in or relating to chromatography |
| US6239262B1 (en) | 1998-01-07 | 2001-05-29 | Rensselaer Polytechnic Institute | Low molecular weight displacers for protein purification in hydrophobic interaction and reversed phase chromatographic systems |
| CA2233725A1 (en) * | 1998-03-31 | 1999-09-30 | Hemosol Inc. | Hemoglobin-hydroxyethyl starch complexes |
| GB9822963D0 (en) * | 1998-10-20 | 1998-12-16 | Agner Erik | Improvements in or relating to chromatography |
| US6573373B1 (en) | 1999-06-29 | 2003-06-03 | Isis Pharmaceuticals, Inc. | High affinity, low molecular weight displacers for oligonucleotide purification |
| US6747132B2 (en) | 2000-11-29 | 2004-06-08 | Apex Biosciences, Inc. | Methods for the synthesis of a modified hemoglobin solution |
| US7195923B2 (en) * | 2001-01-31 | 2007-03-27 | Scripps Laboratories, Inc. | Ratiometric determination of glycated protein |
| ES2545526T3 (es) * | 2001-02-01 | 2015-09-11 | Sigma-Aldrich Co. Llc | Matrices de afinidad mejorada con visibilidad mejorada para aplicaciones de arrastre molecular e inmunoprecipitación |
| US20030153491A1 (en) * | 2002-01-11 | 2003-08-14 | Winslow Robert M. | Methods and compositions for oxygen transport comprising a high oxygen affinity modified hemoglobin |
| US20050164915A1 (en) * | 2002-04-01 | 2005-07-28 | Sangart, Inc. | Compositions for oxygen transport comprising a high oxygen affinity modified hemoglobin |
| US6929747B2 (en) | 2002-02-28 | 2005-08-16 | Renesselaer Polytechnic Institute | High-affinity, low-molecular-mass displacers for ion-exchange chromatography |
| JP3912206B2 (ja) * | 2002-07-05 | 2007-05-09 | 株式会社日立製作所 | 筒内直接燃料噴射装置用燃料ポンプ |
| BRPI0608584B8 (pt) | 2005-03-11 | 2021-05-25 | Wyeth Corp | métodos de recuperar um produto purificado |
| DE112006001012B4 (de) * | 2005-04-22 | 2017-05-18 | Waters Technologies Corp. (N.D.Ges.D. Staates Delaware) | Verfahren für eine auf Massenspektrometrie gerichtete Aufbereitung von Biopolymeren |
| CA2630806C (en) * | 2005-12-02 | 2015-01-06 | Sachem, Inc. | Anion-exchange displacement chromatography process and anionic organic compounds for use as displacer compounds in anion-exchange displacement chromatography process |
| US7976708B2 (en) * | 2008-05-12 | 2011-07-12 | Secretary, Department of Atormic Energy | Innovative cut-and-feed operation for enhancing the performance of ion-exchange chromatographic separation |
| HUE066562T2 (hu) | 2008-08-14 | 2024-08-28 | Genentech Inc | Eljárások szennyezés eltávolítására indigén fehérje kiszorításos ioncserés membrán kromatográfia alkalmazásával |
| EP4786482A2 (en) | 2009-08-06 | 2026-08-05 | F. Hoffmann-La Roche AG | Virus filtration methods |
| US8304248B2 (en) | 2009-11-16 | 2012-11-06 | Torres Anthony R | Protein separation via ion-exchange chromatography and associated methods, systems, and devices |
| HRP20171095T4 (hr) * | 2010-05-25 | 2022-07-22 | F. Hoffmann - La Roche Ag | Postupci pročišćavanja polipeptida |
| JP2014503495A (ja) | 2010-11-15 | 2014-02-13 | バイオジェン アイデック インコーポレイテッド | 過負荷結合および溶出クロマトグラフィによる選択生成物アイソフォームの富化および濃縮 |
| US8293115B2 (en) * | 2011-01-20 | 2012-10-23 | Gordon Rossiter | Ionic impurities rejection and chromatographic purification using ion exchange |
| GB201103363D0 (en) * | 2011-02-28 | 2011-04-13 | Ge Healthcare Uk Ltd | Sample preservation method and sample application substrate |
| KR20140091721A (ko) * | 2011-11-02 | 2014-07-22 | 제넨테크, 인크. | 오버로드 및 용리 크로마토그래피 |
| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| CN103665098B (zh) * | 2012-09-20 | 2015-08-05 | 中国科学院大连化学物理研究所 | 双相柱膜蛋白质微反应器及其应用 |
| DK2994485T3 (en) | 2013-05-06 | 2018-04-23 | Sanofi Sa | CONTINUOUS MULTIPLE STEP PROCEDURE FOR PURIFICATION OF ANTIBODIES |
| EP3546475A1 (en) | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| JP6600123B1 (ja) * | 2018-04-18 | 2019-10-30 | 積水メディカル株式会社 | ヘモグロビン分析方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3971842A (en) * | 1974-08-08 | 1976-07-27 | Atlantic Richfield Company | Continuous displacement chromatographic separation |
| FR2548670B1 (fr) * | 1983-07-07 | 1985-10-25 | Merieux Inst | Procede de preparation des principales proteines du sang hemolyse sous forme non denaturee |
| JPS6075530A (ja) * | 1983-09-30 | 1985-04-27 | Asahi Chem Ind Co Ltd | 金属元素の新しい分離精製方法 |
| CA1244349A (en) * | 1985-06-26 | 1988-11-08 | Jen-Chang Hsia | Purification of hemoglobin and modified hemoglobin by affinity chromatography |
| US5084558A (en) * | 1987-10-13 | 1992-01-28 | Biopure Corporation | Extra pure semi-synthetic blood substitute |
| US5340474A (en) * | 1988-03-24 | 1994-08-23 | Terrapin Technologies, Inc. | Panels of analyte-binding ligands |
| CA1338244C (en) * | 1988-08-17 | 1996-04-09 | Xiang-Fu Wu | Purification of hemoglobin and methemoglobin by bioselective elution |
| US5028696A (en) * | 1988-10-28 | 1991-07-02 | Torres Anthony R | Ion exchange and separation method |
| US5149436A (en) * | 1988-11-28 | 1992-09-22 | Union Oil Company Of California | Continuous displacement chromatographic method |
| IT1232311B (it) * | 1989-09-04 | 1992-01-28 | Sclavo Spa | Metodo di purificazione di composti a struttura peptidica o pseudo peptidica a basso peso molecolare. |
| US5264555A (en) * | 1992-07-14 | 1993-11-23 | Enzon, Inc. | Process for hemoglobin extraction and purification |
-
1993
- 1993-09-21 CA CA002106612A patent/CA2106612C/en not_active Expired - Lifetime
-
1994
- 1994-01-27 US US08/187,316 patent/US5439591A/en not_active Expired - Lifetime
- 1994-09-20 EP EP94926756A patent/EP0668877B1/en not_active Expired - Lifetime
- 1994-09-20 AU AU76503/94A patent/AU681438B2/en not_active Ceased
- 1994-09-20 CZ CZ19951287A patent/CZ287589B6/cs not_active IP Right Cessation
- 1994-09-20 WO PCT/CA1994/000514 patent/WO1995008574A1/en not_active Ceased
- 1994-09-20 SK SK678-95A patent/SK281839B6/sk unknown
- 1994-09-20 PL PL94309034A patent/PL179525B1/pl not_active IP Right Cessation
- 1994-09-20 CN CN94190708A patent/CN1051317C/zh not_active Expired - Fee Related
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|---|---|
| SK67895A3 (enExample) | 1995-09-13 |
| WO1995008574A1 (en) | 1995-03-30 |
| CZ128795A3 (en) | 1995-12-13 |
| SK281839B6 (sk) | 2001-08-06 |
| PL309034A1 (en) | 1995-09-18 |
| EP0668877A1 (en) | 1995-08-30 |
| AU681438B2 (en) | 1997-08-28 |
| CA2106612A1 (en) | 1995-03-22 |
| PL179525B1 (pl) | 2000-09-29 |
| CN1114836A (zh) | 1996-01-10 |
| CN1051317C (zh) | 2000-04-12 |
| EP0668877B1 (en) | 1999-01-20 |
| CZ287589B6 (en) | 2000-12-13 |
| AU7650394A (en) | 1995-04-10 |
| US5439591A (en) | 1995-08-08 |
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