ES2126775T3 - Procedimiento cromatografico de desplazamiento y producto de hemoglobina purificada. - Google Patents
Procedimiento cromatografico de desplazamiento y producto de hemoglobina purificada.Info
- Publication number
- ES2126775T3 ES2126775T3 ES94926756T ES94926756T ES2126775T3 ES 2126775 T3 ES2126775 T3 ES 2126775T3 ES 94926756 T ES94926756 T ES 94926756T ES 94926756 T ES94926756 T ES 94926756T ES 2126775 T3 ES2126775 T3 ES 2126775T3
- Authority
- ES
- Spain
- Prior art keywords
- hemoglobin
- column
- admixtures
- stage
- displacement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/422—Displacement mode
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
LA HEMOGLOBINA ES PURIFICADA DE UNA SOLUCION EN BRUTO, PARA OBTENER UNA SOLUCION ACUOSA QUE CONTIENE AL MENOS EL 99% DE UNA ESPECIE DE HEMOGLOBINA PRESELECCIONADA, POR UN PROCESO DE CROMATOGRAFIA DE DESPLAZAMIENTO DE DOS ETAPAS. UNA DE LAS ETAPAS ES CONDUCIDA BAJO CONDICIONES DE CAMBIO DE ANION, Y LA OTRA BAJO CONDICIONES DE CAMBIO DE CATION. EN AMBAS ETAPAS, LA COLUMNA DE CAMBIO ES SOBRECARGADA PARA DESPLAZAR LAS ESPECIES DE HEMOGLOBINA DE ELLO CON CONTAMINANTES QUE TIENEN AFINIDAD MAS GRANDE POR LA COLUMNA, Y USANDO LA SOLUCION DE HEMOGLOBINA IMPURA COMO EL DESPLAZADOR. NORMALMENTE, EL CAMBIO DE ANION ES CONDUCIDO PRIMERO, CON LOS CONTAMINANTES MAS ACIDICOS QUE LA HEMOGLOBINA QUE DESPLAZA LA HEMOGLOBINA DE LA COLUMNA Y ELLOS MISMOS PERMANECEN UNIDOS A LA COLUMNA PARA LA SEPARACION. EL PROCESO DE CAMBIO DE CATION ES CONDUCIDO DESPUES, EN EL ELUYENTE DE LA PRIMERA COLUMNA, Y EN ESTA ETAPA, LOS CONTAMINANTES MAS BASICOS DESPLAZAN LA HEMOGLOBINA DESDE LA COLUMNA BAJO CONDICIONES DE SOBRECARGA, PARA PRODUCIR UNA SOLUCION DE HEMOGLOBINA SUSTANCIALMENTE PURA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002106612A CA2106612C (en) | 1993-09-21 | 1993-09-21 | Displacement chromatography process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2126775T3 true ES2126775T3 (es) | 1999-04-01 |
Family
ID=4152343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES94926756T Expired - Lifetime ES2126775T3 (es) | 1993-09-21 | 1994-09-20 | Procedimiento cromatografico de desplazamiento y producto de hemoglobina purificada. |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JP4057049B2 (es) |
| KR (1) | KR100361894B1 (es) |
| AT (1) | ATE175975T1 (es) |
| DE (1) | DE69416103T2 (es) |
| DK (1) | DK0668877T3 (es) |
| ES (1) | ES2126775T3 (es) |
| HU (1) | HU218159B (es) |
| RU (1) | RU2145873C1 (es) |
| UA (1) | UA35599C2 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE112006001012B4 (de) * | 2005-04-22 | 2017-05-18 | Waters Technologies Corp. (N.D.Ges.D. Staates Delaware) | Verfahren für eine auf Massenspektrometrie gerichtete Aufbereitung von Biopolymeren |
| CA2770235C (en) | 2009-08-06 | 2021-06-29 | Genentech, Inc. | Method to improve virus removal in protein purification |
| EP2640483A1 (en) * | 2010-11-15 | 2013-09-25 | Biogen Idec Inc. | Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography |
| JP6605202B2 (ja) | 2011-12-22 | 2019-11-13 | ジェネンテック， インコーポレイテッド | イオン交換膜クロマトグラフィー |
| SG11201508968XA (en) * | 2013-05-06 | 2015-11-27 | Sanofi Sa | Continuous multistep process for purifying antibodies |
| TW202128264A (zh) * | 2019-09-25 | 2021-08-01 | 瑞士商赫孚孟拉羅股份公司 | 使用經預測之溶離緩衝鹽濃度的陽離子層析法 |
| CN114712894B (zh) * | 2022-04-08 | 2024-04-30 | 无锡博慧斯生物医药科技有限公司 | 一种糖化血红蛋白层析柱的活化液、活化方法和应用 |
-
1994
- 1994-09-20 JP JP50945895A patent/JP4057049B2/ja not_active Expired - Fee Related
- 1994-09-20 AT AT94926756T patent/ATE175975T1/de not_active IP Right Cessation
- 1994-09-20 ES ES94926756T patent/ES2126775T3/es not_active Expired - Lifetime
- 1994-09-20 RU RU95112503A patent/RU2145873C1/ru not_active IP Right Cessation
- 1994-09-20 UA UA95058439A patent/UA35599C2/uk unknown
- 1994-09-20 HU HU9501479A patent/HU218159B/hu not_active IP Right Cessation
- 1994-09-20 DK DK94926756T patent/DK0668877T3/da active
- 1994-09-20 DE DE69416103T patent/DE69416103T2/de not_active Expired - Fee Related
- 1994-09-21 KR KR1019950701487A patent/KR100361894B1/ko not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DE69416103D1 (de) | 1999-03-04 |
| HU218159B (hu) | 2000-06-28 |
| HUT72924A (en) | 1996-06-28 |
| JPH08503714A (ja) | 1996-04-23 |
| ATE175975T1 (de) | 1999-02-15 |
| JP4057049B2 (ja) | 2008-03-05 |
| DK0668877T3 (da) | 1999-09-13 |
| RU95112503A (ru) | 1996-12-20 |
| DE69416103T2 (de) | 1999-08-05 |
| RU2145873C1 (ru) | 2000-02-27 |
| UA35599C2 (uk) | 2001-04-16 |
| HU9501479D0 (en) | 1995-07-28 |
| KR100361894B1 (ko) | 2003-05-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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