CN105116138A - 用于鉴定细胞表面抗原的免疫结合剂的方法 - Google Patents

用于鉴定细胞表面抗原的免疫结合剂的方法 Download PDF

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Publication number
CN105116138A
CN105116138A CN201510261116.5A CN201510261116A CN105116138A CN 105116138 A CN105116138 A CN 105116138A CN 201510261116 A CN201510261116 A CN 201510261116A CN 105116138 A CN105116138 A CN 105116138A
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CN
China
Prior art keywords
cell
antibody
antigen
immunoconjugator
object antigen
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Pending
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CN201510261116.5A
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English (en)
Chinese (zh)
Inventor
V·胡尔曼-考提尔
D·厄里什
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Esbatech a Novartis Co LLC
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Esbatech An Alcon Biomedical Research Unit LLC
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Priority claimed from PCT/CH2009/000222 external-priority patent/WO2009155726A2/en
Application filed by Esbatech An Alcon Biomedical Research Unit LLC filed Critical Esbatech An Alcon Biomedical Research Unit LLC
Priority claimed from CN2010800034510A external-priority patent/CN102239181A/zh
Publication of CN105116138A publication Critical patent/CN105116138A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201510261116.5A 2009-02-24 2010-02-22 用于鉴定细胞表面抗原的免疫结合剂的方法 Pending CN105116138A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US15510509P 2009-02-24 2009-02-24
US15504109P 2009-02-24 2009-02-24
US61/155,105 2009-02-24
US61/155,041 2009-02-24
CH832/09 2009-06-02
CH8322009 2009-06-02
PCT/CH2009/000222 WO2009155726A2 (en) 2008-06-25 2009-06-25 Humanization of rabbit antibodies using a universal antibody framework
CHPCT/CH2009/000222 2009-06-25
CN2010800034510A CN102239181A (zh) 2009-02-24 2010-02-22 用于鉴定细胞表面抗原的免疫结合剂的方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2010800034510A Division CN102239181A (zh) 2009-02-24 2010-02-22 用于鉴定细胞表面抗原的免疫结合剂的方法

Publications (1)

Publication Number Publication Date
CN105116138A true CN105116138A (zh) 2015-12-02

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CN201510261116.5A Pending CN105116138A (zh) 2009-02-24 2010-02-22 用于鉴定细胞表面抗原的免疫结合剂的方法

Country Status (12)

Country Link
JP (3) JP6161655B2 (ko)
KR (4) KR20180005752A (ko)
CN (1) CN105116138A (ko)
BR (1) BRPI1005398A2 (ko)
CL (1) CL2011001453A1 (ko)
ES (1) ES2618181T3 (ko)
IL (4) IL226955A (ko)
MX (1) MX352922B (ko)
PH (1) PH12014502468B1 (ko)
RU (2) RU2577965C2 (ko)
SG (3) SG10201408247SA (ko)
ZA (1) ZA201103125B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016462A (zh) * 2019-02-20 2019-07-16 优睿赛思(武汉)生物科技有限公司 从脾脏细胞中高效分离单个抗原特异性b淋巴细胞的方法

Citations (9)

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JPS63253098A (ja) * 1987-04-10 1988-10-20 Ube Ind Ltd ヒト抗体に対するモノクロ−ナル抗体、その製法及び用途
WO1995003770A1 (en) * 1993-08-02 1995-02-09 The Board Of Trustees Of The Leland Stanford Junior University Methods for b-cell population control
KR0119909B1 (ko) * 1993-10-05 1997-10-20 김은영 플로우 사이토미터를 이용한 고상형 면역 측정방법
CN1396182A (zh) * 2002-07-31 2003-02-12 陕西超英生物医学研究开发有限公司 人内皮生长因子受体2蛋白全人单克隆抗体及其制备方法
WO2004102198A2 (en) * 2003-05-15 2004-11-25 Cytos Biotechnology Ag Selection of b cells with specificity of interest: method of preparation and use
CN1662556A (zh) * 2002-05-22 2005-08-31 艾斯巴技术股份公司 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
WO2007003041A1 (en) * 2005-07-01 2007-01-11 John Schrader Methods of isolating cells and generating monoclonal antibodies
US20070122852A1 (en) * 2002-11-13 2007-05-31 Micromet Ag Method for identifying antigen specific b cells
WO2007143168A2 (en) * 2006-06-02 2007-12-13 Regeneron Pharmaceuticals, Inc. High affinity antibodies to human il-6 receptor

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IL91948A (en) 1988-10-26 1994-05-30 Immunomedics Removal of immunoreactive components from a sample
CA2761116A1 (en) * 1995-04-27 1996-10-31 Amgen Fremont Inc. Human antibodies derived from immunized xenomice
WO1998002462A1 (en) * 1996-07-16 1998-01-22 Morphosys Gesellschaft Für Proteinoptimierung Mbh Immunoglobulin superfamily domains and fragments with increased solubility
EP1539947A4 (en) * 2002-08-15 2006-09-06 Epitomics Inc HUMANIZED RABBIT ANTIBODIES
JP4336812B2 (ja) * 2003-03-07 2009-09-30 博夫 岩田 細胞に発現する表面抗原を迅速に同定するための分析方法
EP1651659A4 (en) * 2003-08-07 2008-09-17 Epitomics Inc METHOD FOR HUMANIZATION OF MONOCLONAL RABBIT ANTIBODIES
US20070134249A1 (en) * 2005-12-08 2007-06-14 Genitope Corporation Combination therapy and antibody panels
KR101363695B1 (ko) * 2005-12-28 2014-02-14 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 항페리오스틴에 대한 항체, 및 페리오스틴이 관여하는 질환의 예방 또는 치료를 위해 그것을 함유하는 약제학적 조성물
WO2008004834A1 (en) * 2006-07-06 2008-01-10 Isu Abxis Co., Ltd Humanized monoclonal antibody highly binding to epidermal growth factor receptor, egf receptor

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JPS63253098A (ja) * 1987-04-10 1988-10-20 Ube Ind Ltd ヒト抗体に対するモノクロ−ナル抗体、その製法及び用途
WO1995003770A1 (en) * 1993-08-02 1995-02-09 The Board Of Trustees Of The Leland Stanford Junior University Methods for b-cell population control
KR0119909B1 (ko) * 1993-10-05 1997-10-20 김은영 플로우 사이토미터를 이용한 고상형 면역 측정방법
CN1662556A (zh) * 2002-05-22 2005-08-31 艾斯巴技术股份公司 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN1396182A (zh) * 2002-07-31 2003-02-12 陕西超英生物医学研究开发有限公司 人内皮生长因子受体2蛋白全人单克隆抗体及其制备方法
US20070122852A1 (en) * 2002-11-13 2007-05-31 Micromet Ag Method for identifying antigen specific b cells
WO2004102198A2 (en) * 2003-05-15 2004-11-25 Cytos Biotechnology Ag Selection of b cells with specificity of interest: method of preparation and use
WO2007003041A1 (en) * 2005-07-01 2007-01-11 John Schrader Methods of isolating cells and generating monoclonal antibodies
WO2007143168A2 (en) * 2006-06-02 2007-12-13 Regeneron Pharmaceuticals, Inc. High affinity antibodies to human il-6 receptor

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PAUL A. LALOR，ET AL: "Functional and molecular characterization of single, (4-hydroxy -3-nitrophenyl) acetyl (NP)-specific, IgG1+ B cells from antibody-secreting and memory B cell pathways in the C57BL/6 immune response to NP", 《EUR. J. IMMUNOL. 》 *
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016462A (zh) * 2019-02-20 2019-07-16 优睿赛思(武汉)生物科技有限公司 从脾脏细胞中高效分离单个抗原特异性b淋巴细胞的方法
CN110016462B (zh) * 2019-02-20 2020-04-24 优睿赛思(武汉)生物科技有限公司 从脾脏细胞中高效分离单个抗原特异性b淋巴细胞的方法

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Publication number Publication date
RU2577965C2 (ru) 2016-03-20
CL2011001453A1 (es) 2012-02-24
BRPI1005398A2 (pt) 2019-03-26
JP2017192384A (ja) 2017-10-26
JP2018196395A (ja) 2018-12-13
IL263158B (en) 2019-10-31
ES2618181T3 (es) 2017-06-21
KR20180005752A (ko) 2018-01-16
RU2011124039A (ru) 2012-12-20
KR101817279B1 (ko) 2018-01-10
IL226955A0 (en) 2013-07-31
IL226956A (en) 2015-04-30
RU2017135831A (ru) 2019-04-09
SG10201708233VA (en) 2017-11-29
PH12014502468A1 (en) 2016-01-18
MX352922B (es) 2017-12-14
KR20160150642A (ko) 2016-12-30
SG10201408247SA (en) 2015-02-27
SG2014014864A (en) 2014-09-26
KR20190020202A (ko) 2019-02-27
RU2017135831A3 (ko) 2019-04-09
PH12014502468B1 (en) 2016-01-18
IL226955A (en) 2015-04-30
KR101689791B1 (ko) 2016-12-26
JP6161655B2 (ja) 2017-07-12
ZA201103125B (en) 2012-08-29
KR20160022397A (ko) 2016-02-29
IL263158A (en) 2018-12-31
JP2015146825A (ja) 2015-08-20
IL237985B (en) 2018-12-31
JP6604989B2 (ja) 2019-11-13
IL226956A0 (en) 2013-07-31
KR102059866B1 (ko) 2019-12-27

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