CN105051189B - 具有改善特性的酰基-acp还原酶 - Google Patents

具有改善特性的酰基-acp还原酶 Download PDF

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Publication number
CN105051189B
CN105051189B CN201480004992.3A CN201480004992A CN105051189B CN 105051189 B CN105051189 B CN 105051189B CN 201480004992 A CN201480004992 A CN 201480004992A CN 105051189 B CN105051189 B CN 105051189B
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Prior art keywords
fatty
aar
fatty alcohol
seq
polypeptide
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Chinese (zh)
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CN105051189A (zh
Inventor
M·鲁德
N·特里安
A·席尔默
J·加诺
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Genomatica Inc
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REG Life Sciences LLC
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Priority to CN201910169609.4A priority Critical patent/CN109897834A/zh
Priority to CN201910693927.0A priority patent/CN110387352A/zh
Publication of CN105051189A publication Critical patent/CN105051189A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0108—Long-chain acyl-[acyl-carrier-protein] reductase (1.2.1.80)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
CN201480004992.3A 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶 Active CN105051189B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201910169609.4A CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910693927.0A CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361753273P 2013-01-16 2013-01-16
US61/753,273 2013-01-16
PCT/US2014/011859 WO2014113571A2 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN201910169609.4A Division CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910693927.0A Division CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

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CN105051189A CN105051189A (zh) 2015-11-11
CN105051189B true CN105051189B (zh) 2019-08-06

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CN201480004992.3A Active CN105051189B (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910693927.0A Pending CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910169609.4A Pending CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

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CN201910693927.0A Pending CN110387352A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶
CN201910169609.4A Pending CN109897834A (zh) 2013-01-16 2014-01-16 具有改善特性的酰基-acp还原酶

Country Status (13)

Country Link
US (6) US9683219B2 (cg-RX-API-DMAC7.html)
EP (3) EP2946009B1 (cg-RX-API-DMAC7.html)
JP (5) JP6063585B2 (cg-RX-API-DMAC7.html)
KR (4) KR102082247B1 (cg-RX-API-DMAC7.html)
CN (3) CN105051189B (cg-RX-API-DMAC7.html)
AU (3) AU2014207522B2 (cg-RX-API-DMAC7.html)
BR (1) BR112015017005A2 (cg-RX-API-DMAC7.html)
CA (2) CA3143642A1 (cg-RX-API-DMAC7.html)
CO (1) CO7461134A2 (cg-RX-API-DMAC7.html)
ES (2) ES2600354T3 (cg-RX-API-DMAC7.html)
MX (2) MX346830B (cg-RX-API-DMAC7.html)
MY (1) MY167434A (cg-RX-API-DMAC7.html)
WO (1) WO2014113571A2 (cg-RX-API-DMAC7.html)

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WO2014026162A1 (en) 2012-08-10 2014-02-13 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
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US10047383B2 (en) 2013-03-15 2018-08-14 Cargill, Incorporated Bioproduction of chemicals
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
BR112017001105A2 (pt) * 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
JP2020506702A (ja) 2017-02-02 2020-03-05 カーギル インコーポレイテッド Ｃ６−ｃ１０脂肪酸誘導体を生成する遺伝子組み換え細胞
KR102677413B1 (ko) 2017-04-03 2024-06-24 게노마티카 인코포레이티드 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

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WO2011006137A2 (en) * 2009-07-09 2011-01-13 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
CN102695799A (zh) * 2009-09-27 2012-09-26 Opx生物工艺学公司 用于制备3-羟基丙酸和其它产物的方法
CN102719467A (zh) * 2012-07-09 2012-10-10 武汉大学 一种利用脂肪酰acp还原酶生物合成脂肪醇的方法

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Also Published As

Publication number Publication date
CN105051189A (zh) 2015-11-11
EP3103867B1 (en) 2018-05-16
JP2021006067A (ja) 2021-01-21
JP2017209118A (ja) 2017-11-30
KR101746040B1 (ko) 2017-06-13
WO2014113571A2 (en) 2014-07-24
CO7461134A2 (es) 2015-11-30
JP6211162B2 (ja) 2017-10-11
AU2014207522B2 (en) 2016-12-15
US9873865B2 (en) 2018-01-23
EP2946009B1 (en) 2016-07-27
CA3143642A1 (en) 2014-07-24
CN109897834A (zh) 2019-06-18
JP2016503663A (ja) 2016-02-08
AU2014207522A1 (en) 2015-05-07
US9683219B2 (en) 2017-06-20
JP2017042170A (ja) 2017-03-02
BR112015017005A2 (pt) 2017-08-15
CA2898317C (en) 2022-03-01
JP2019141083A (ja) 2019-08-29
JP7094343B2 (ja) 2022-07-01
AU2019202790A1 (en) 2019-05-16
EP3103867A1 (en) 2016-12-14
US20200010813A1 (en) 2020-01-09
KR20200020998A (ko) 2020-02-26
MX346830B (es) 2017-04-03
JP6063585B2 (ja) 2017-01-18
EP2946009A2 (en) 2015-11-25
ES2600354T3 (es) 2017-02-08
US20160348080A1 (en) 2016-12-01
KR20150105471A (ko) 2015-09-16
US10208294B2 (en) 2019-02-19
US20170342388A1 (en) 2017-11-30
US20150361454A1 (en) 2015-12-17
US12173329B2 (en) 2024-12-24
CN110387352A (zh) 2019-10-29
US11130944B2 (en) 2021-09-28
KR20210072147A (ko) 2021-06-16
KR102265148B1 (ko) 2021-06-15
MX356997B (es) 2018-06-22
AU2017200596B2 (en) 2019-01-24
EP3385375B1 (en) 2021-06-16
ES2675224T3 (es) 2018-07-09
JP6523393B2 (ja) 2019-05-29
KR20170066694A (ko) 2017-06-14
KR102082247B1 (ko) 2020-02-28
JP6926143B2 (ja) 2021-08-25
CA2898317A1 (en) 2014-07-24
MY167434A (en) 2018-08-28
US20220090028A1 (en) 2022-03-24
MX2015009138A (es) 2015-11-06
WO2014113571A3 (en) 2014-11-13
AU2017200596A1 (en) 2017-02-23
EP3385375A1 (en) 2018-10-10
US20240117324A1 (en) 2024-04-11

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Patentee after: Genomatica, Inc.

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Patentee before: REG Life Sciences Co.,Ltd.