CA3143642A1 - Acyl-acp reductase with improved properties - Google Patents

Acyl-acp reductase with improved properties

Info

Publication number
CA3143642A1
CA3143642A1 CA3143642A CA3143642A CA3143642A1 CA 3143642 A1 CA3143642 A1 CA 3143642A1 CA 3143642 A CA3143642 A CA 3143642A CA 3143642 A CA3143642 A CA 3143642A CA 3143642 A1 CA3143642 A1 CA 3143642A1
Authority
CA
Canada
Prior art keywords
mutation
aar polypeptide
fatty alcohol
variant aar
fatty
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3143642A
Other languages
English (en)
Inventor
Mathew Rude
Na Trinh
Andreas Schirmer
Jacob GANO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genomatica Inc
Original Assignee
Genomatica Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica Inc filed Critical Genomatica Inc
Publication of CA3143642A1 publication Critical patent/CA3143642A1/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/24Preparation of oxygen-containing organic compounds containing a carbonyl group
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0108Long-chain acyl-[acyl-carrier-protein] reductase (1.2.1.80)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
CA3143642A 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties Pending CA3143642A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361753273P 2013-01-16 2013-01-16
US61/753,273 2013-01-16
CA2898317A CA2898317C (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2898317A Division CA2898317C (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Publications (1)

Publication Number Publication Date
CA3143642A1 true CA3143642A1 (en) 2014-07-24

Family

ID=50473752

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3143642A Pending CA3143642A1 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties
CA2898317A Active CA2898317C (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2898317A Active CA2898317C (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Country Status (13)

Country Link
US (6) US9683219B2 (cg-RX-API-DMAC7.html)
EP (3) EP2946009B1 (cg-RX-API-DMAC7.html)
JP (5) JP6063585B2 (cg-RX-API-DMAC7.html)
KR (4) KR102082247B1 (cg-RX-API-DMAC7.html)
CN (3) CN105051189B (cg-RX-API-DMAC7.html)
AU (3) AU2014207522B2 (cg-RX-API-DMAC7.html)
BR (1) BR112015017005A2 (cg-RX-API-DMAC7.html)
CA (2) CA3143642A1 (cg-RX-API-DMAC7.html)
CO (1) CO7461134A2 (cg-RX-API-DMAC7.html)
ES (2) ES2600354T3 (cg-RX-API-DMAC7.html)
MX (2) MX346830B (cg-RX-API-DMAC7.html)
MY (1) MY167434A (cg-RX-API-DMAC7.html)
WO (1) WO2014113571A2 (cg-RX-API-DMAC7.html)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2775390C (en) 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
WO2014026162A1 (en) 2012-08-10 2014-02-13 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN105051189B (zh) * 2013-01-16 2019-08-06 Reg生命科学有限责任公司 具有改善特性的酰基-acp还原酶
US10047383B2 (en) 2013-03-15 2018-08-14 Cargill, Incorporated Bioproduction of chemicals
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
BR112017001105A2 (pt) * 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
JP2020506702A (ja) 2017-02-02 2020-03-05 カーギル インコーポレイテッド C6−c10脂肪酸誘導体を生成する遺伝子組み換え細胞
KR102677413B1 (ko) 2017-04-03 2024-06-24 게노마티카 인코포레이티드 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
BR0108198A (pt) * 2000-02-09 2003-03-25 Basf Ag ácido nucleico isolado, construção de gene, sequência de aminoácidos, vetor, organismo, anticorpo, molécula de ácido nucleico anti-sentido, processo para a preparação de pufas, óleo, lipìdeo ou ácido graxo ou uma fração destes, composição, uso da composição oleaginosa, lipìdica ou de ácido graxo, kit, e, processo para identificar um antagonista de elongases
EP2194119A3 (en) 2006-05-19 2010-09-22 LS9, Inc. Production of fatty acids and derivatives thereof
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
WO2008113041A2 (en) 2007-03-14 2008-09-18 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
CA2678915C (en) 2007-03-28 2019-04-09 Ls9, Inc. Enhanced production of fatty acid derivatives
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
WO2009085278A1 (en) 2007-12-21 2009-07-09 Ls9, Inc. Methods and compositions for producing olefins
CN102027108A (zh) 2008-05-16 2011-04-20 Ls9公司 产生碳氢化合物的方法和组合物
US8232924B2 (en) 2008-05-23 2012-07-31 Alliant Techsystems Inc. Broadband patch antenna and antenna system
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
EP2796559B1 (en) 2008-10-28 2018-09-12 REG Life Sciences, LLC Microbial cell for producing fatty alcohols
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
EP2432890B1 (en) * 2009-05-22 2015-09-02 Codexis, Inc. Engineered biosynthesis of fatty alcohols
US7794969B1 (en) * 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
JP5622154B2 (ja) * 2009-09-07 2014-11-12 独立行政法人情報通信研究機構 複数のマッハツェンダー干渉計を有する光変調器の特性評価方法
ES2707747T3 (es) 2009-09-25 2019-04-04 Reg Life Sciences Llc Producción de derivados de ácidos grasos
CA2775390C (en) 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US20110250663A1 (en) * 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
AU2011349444B2 (en) * 2010-12-20 2016-06-30 Lumen Bioscience, Inc. Modified photosynthetic microorganisms for producing lipids
WO2012096686A1 (en) * 2011-01-14 2012-07-19 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
CN102719467A (zh) * 2012-07-09 2012-10-10 武汉大学 一种利用脂肪酰acp还原酶生物合成脂肪醇的方法
CN105051189B (zh) 2013-01-16 2019-08-06 Reg生命科学有限责任公司 具有改善特性的酰基-acp还原酶
JP7478517B2 (ja) 2019-04-24 2024-05-07 三菱商事ライフサイエンス株式会社 脂質活性代謝改善組成物

Also Published As

Publication number Publication date
CN105051189A (zh) 2015-11-11
EP3103867B1 (en) 2018-05-16
JP2021006067A (ja) 2021-01-21
JP2017209118A (ja) 2017-11-30
KR101746040B1 (ko) 2017-06-13
WO2014113571A2 (en) 2014-07-24
CO7461134A2 (es) 2015-11-30
JP6211162B2 (ja) 2017-10-11
AU2014207522B2 (en) 2016-12-15
US9873865B2 (en) 2018-01-23
EP2946009B1 (en) 2016-07-27
CN109897834A (zh) 2019-06-18
JP2016503663A (ja) 2016-02-08
AU2014207522A1 (en) 2015-05-07
US9683219B2 (en) 2017-06-20
JP2017042170A (ja) 2017-03-02
BR112015017005A2 (pt) 2017-08-15
CA2898317C (en) 2022-03-01
JP2019141083A (ja) 2019-08-29
JP7094343B2 (ja) 2022-07-01
AU2019202790A1 (en) 2019-05-16
EP3103867A1 (en) 2016-12-14
US20200010813A1 (en) 2020-01-09
KR20200020998A (ko) 2020-02-26
MX346830B (es) 2017-04-03
JP6063585B2 (ja) 2017-01-18
EP2946009A2 (en) 2015-11-25
ES2600354T3 (es) 2017-02-08
US20160348080A1 (en) 2016-12-01
KR20150105471A (ko) 2015-09-16
US10208294B2 (en) 2019-02-19
US20170342388A1 (en) 2017-11-30
CN105051189B (zh) 2019-08-06
US20150361454A1 (en) 2015-12-17
US12173329B2 (en) 2024-12-24
CN110387352A (zh) 2019-10-29
US11130944B2 (en) 2021-09-28
KR20210072147A (ko) 2021-06-16
KR102265148B1 (ko) 2021-06-15
MX356997B (es) 2018-06-22
AU2017200596B2 (en) 2019-01-24
EP3385375B1 (en) 2021-06-16
ES2675224T3 (es) 2018-07-09
JP6523393B2 (ja) 2019-05-29
KR20170066694A (ko) 2017-06-14
KR102082247B1 (ko) 2020-02-28
JP6926143B2 (ja) 2021-08-25
CA2898317A1 (en) 2014-07-24
MY167434A (en) 2018-08-28
US20220090028A1 (en) 2022-03-24
MX2015009138A (es) 2015-11-06
WO2014113571A3 (en) 2014-11-13
AU2017200596A1 (en) 2017-02-23
EP3385375A1 (en) 2018-10-10
US20240117324A1 (en) 2024-04-11

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