JP6063585B2 - 改良された特性を有するアシル−ａｃｐレダクターゼ - Google Patents

改良された特性を有するアシル−ａｃｐレダクターゼ Download PDF

Info

Publication number
JP6063585B2
JP6063585B2 JP2015553830A JP2015553830A JP6063585B2 JP 6063585 B2 JP6063585 B2 JP 6063585B2 JP 2015553830 A JP2015553830 A JP 2015553830A JP 2015553830 A JP2015553830 A JP 2015553830A JP 6063585 B2 JP6063585 B2 JP 6063585B2
Authority
JP
Japan
Prior art keywords
mutation
fatty
polypeptide
aar
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015553830A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016503663A (ja
JP2016503663A5 (cg-RX-API-DMAC7.html
Inventor
マシュー リュード
マシュー リュード
ナ トリン
ナ トリン
アンドレアス シルマー
アンドレアス シルマー
ジェーコブ ガーノ
ジェーコブ ガーノ
Original Assignee
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー, アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー filed Critical アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
Publication of JP2016503663A publication Critical patent/JP2016503663A/ja
Publication of JP2016503663A5 publication Critical patent/JP2016503663A5/ja
Application granted granted Critical
Publication of JP6063585B2 publication Critical patent/JP6063585B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0108—Long-chain acyl-[acyl-carrier-protein] reductase (1.2.1.80)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
JP2015553830A 2013-01-16 2014-01-16 改良された特性を有するアシル−ａｃｐレダクターゼ Active JP6063585B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361753273P 2013-01-16 2013-01-16
US61/753,273 2013-01-16
PCT/US2014/011859 WO2014113571A2 (en) 2013-01-16 2014-01-16 Acyl-acp reductase with improved properties

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016202155A Division JP6211162B2 (ja) 2013-01-16 2016-10-14 改良された特性を有するアシル−ａｃｐレダクターゼ

Publications (3)

Publication Number Publication Date
JP2016503663A JP2016503663A (ja) 2016-02-08
JP2016503663A5 JP2016503663A5 (cg-RX-API-DMAC7.html) 2016-09-29
JP6063585B2 true JP6063585B2 (ja) 2017-01-18

Family

ID=50473752

Family Applications (5)

Application Number Title Priority Date Filing Date
JP2015553830A Active JP6063585B2 (ja) 2013-01-16 2014-01-16 改良された特性を有するアシル−ａｃｐレダクターゼ
JP2016202155A Expired - Fee Related JP6211162B2 (ja) 2013-01-16 2016-10-14 改良された特性を有するアシル−ａｃｐレダクターゼ
JP2017174449A Expired - Fee Related JP6523393B2 (ja) 2013-01-16 2017-09-12 改良された特性を有するアシル−ａｃｐレダクターゼ
JP2019084119A Active JP6926143B2 (ja) 2013-01-16 2019-04-25 改良された特性を有するアシル−ａｃｐレダクターゼ
JP2020178581A Active JP7094343B2 (ja) 2013-01-16 2020-10-26 改良された特性を有するアシル－ａｃｐレダクターゼ

Family Applications After (4)

Application Number Title Priority Date Filing Date
JP2016202155A Expired - Fee Related JP6211162B2 (ja) 2013-01-16 2016-10-14 改良された特性を有するアシル−ａｃｐレダクターゼ
JP2017174449A Expired - Fee Related JP6523393B2 (ja) 2013-01-16 2017-09-12 改良された特性を有するアシル−ａｃｐレダクターゼ
JP2019084119A Active JP6926143B2 (ja) 2013-01-16 2019-04-25 改良された特性を有するアシル−ａｃｐレダクターゼ
JP2020178581A Active JP7094343B2 (ja) 2013-01-16 2020-10-26 改良された特性を有するアシル－ａｃｐレダクターゼ

Country Status (13)

Country Link
US (6) US9683219B2 (cg-RX-API-DMAC7.html)
EP (3) EP2946009B1 (cg-RX-API-DMAC7.html)
JP (5) JP6063585B2 (cg-RX-API-DMAC7.html)
KR (4) KR102082247B1 (cg-RX-API-DMAC7.html)
CN (3) CN105051189B (cg-RX-API-DMAC7.html)
AU (3) AU2014207522B2 (cg-RX-API-DMAC7.html)
BR (1) BR112015017005A2 (cg-RX-API-DMAC7.html)
CA (2) CA3143642A1 (cg-RX-API-DMAC7.html)
CO (1) CO7461134A2 (cg-RX-API-DMAC7.html)
ES (2) ES2600354T3 (cg-RX-API-DMAC7.html)
MX (2) MX346830B (cg-RX-API-DMAC7.html)
MY (1) MY167434A (cg-RX-API-DMAC7.html)
WO (1) WO2014113571A2 (cg-RX-API-DMAC7.html)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2775390C (en) 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
WO2014026162A1 (en) 2012-08-10 2014-02-13 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN105051189B (zh) * 2013-01-16 2019-08-06 Reg生命科学有限责任公司 具有改善特性的酰基-acp还原酶
US10047383B2 (en) 2013-03-15 2018-08-14 Cargill, Incorporated Bioproduction of chemicals
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
BR112017001105A2 (pt) * 2014-07-18 2018-07-24 REG Life Sciences, LLC produção microbiana de dióis graxos
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
JP2020506702A (ja) 2017-02-02 2020-03-05 カーギル インコーポレイテッド Ｃ６−ｃ１０脂肪酸誘導体を生成する遺伝子組み換え細胞
KR102677413B1 (ko) 2017-04-03 2024-06-24 게노마티카 인코포레이티드 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
US12221644B2 (en) 2018-08-31 2025-02-11 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
EP4493683A1 (en) 2022-03-16 2025-01-22 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
BR0108198A (pt) * 2000-02-09 2003-03-25 Basf Ag ácido nucleico isolado, construção de gene, sequência de aminoácidos, vetor, organismo, anticorpo, molécula de ácido nucleico anti-sentido, processo para a preparação de pufas, óleo, lipìdeo ou ácido graxo ou uma fração destes, composição, uso da composição oleaginosa, lipìdica ou de ácido graxo, kit, e, processo para identificar um antagonista de elongases
EP2194119A3 (en) 2006-05-19 2010-09-22 LS9, Inc. Production of fatty acids and derivatives thereof
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
WO2008113041A2 (en) 2007-03-14 2008-09-18 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
CA2678915C (en) 2007-03-28 2019-04-09 Ls9, Inc. Enhanced production of fatty acid derivatives
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
WO2009085278A1 (en) 2007-12-21 2009-07-09 Ls9, Inc. Methods and compositions for producing olefins
CN102027108A (zh) 2008-05-16 2011-04-20 Ls9公司 产生碳氢化合物的方法和组合物
US8232924B2 (en) 2008-05-23 2012-07-31 Alliant Techsystems Inc. Broadband patch antenna and antenna system
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
EP2796559B1 (en) 2008-10-28 2018-09-12 REG Life Sciences, LLC Microbial cell for producing fatty alcohols
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
EP2432890B1 (en) * 2009-05-22 2015-09-02 Codexis, Inc. Engineered biosynthesis of fatty alcohols
US7794969B1 (en) * 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
JP5622154B2 (ja) * 2009-09-07 2014-11-12 独立行政法人情報通信研究機構 複数のマッハツェンダー干渉計を有する光変調器の特性評価方法
ES2707747T3 (es) 2009-09-25 2019-04-04 Reg Life Sciences Llc Producción de derivados de ácidos grasos
CA2775390C (en) 2009-09-27 2021-06-29 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US20110250663A1 (en) * 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
AU2011349444B2 (en) * 2010-12-20 2016-06-30 Lumen Bioscience, Inc. Modified photosynthetic microorganisms for producing lipids
WO2012096686A1 (en) * 2011-01-14 2012-07-19 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
CN102719467A (zh) * 2012-07-09 2012-10-10 武汉大学 一种利用脂肪酰acp还原酶生物合成脂肪醇的方法
CN105051189B (zh) 2013-01-16 2019-08-06 Reg生命科学有限责任公司 具有改善特性的酰基-acp还原酶
JP7478517B2 (ja) 2019-04-24 2024-05-07 三菱商事ライフサイエンス株式会社 脂質活性代謝改善組成物

Also Published As

Publication number Publication date
CN105051189A (zh) 2015-11-11
EP3103867B1 (en) 2018-05-16
JP2021006067A (ja) 2021-01-21
JP2017209118A (ja) 2017-11-30
KR101746040B1 (ko) 2017-06-13
WO2014113571A2 (en) 2014-07-24
CO7461134A2 (es) 2015-11-30
JP6211162B2 (ja) 2017-10-11
AU2014207522B2 (en) 2016-12-15
US9873865B2 (en) 2018-01-23
EP2946009B1 (en) 2016-07-27
CA3143642A1 (en) 2014-07-24
CN109897834A (zh) 2019-06-18
JP2016503663A (ja) 2016-02-08
AU2014207522A1 (en) 2015-05-07
US9683219B2 (en) 2017-06-20
JP2017042170A (ja) 2017-03-02
BR112015017005A2 (pt) 2017-08-15
CA2898317C (en) 2022-03-01
JP2019141083A (ja) 2019-08-29
JP7094343B2 (ja) 2022-07-01
AU2019202790A1 (en) 2019-05-16
EP3103867A1 (en) 2016-12-14
US20200010813A1 (en) 2020-01-09
KR20200020998A (ko) 2020-02-26
MX346830B (es) 2017-04-03
EP2946009A2 (en) 2015-11-25
ES2600354T3 (es) 2017-02-08
US20160348080A1 (en) 2016-12-01
KR20150105471A (ko) 2015-09-16
US10208294B2 (en) 2019-02-19
US20170342388A1 (en) 2017-11-30
CN105051189B (zh) 2019-08-06
US20150361454A1 (en) 2015-12-17
US12173329B2 (en) 2024-12-24
CN110387352A (zh) 2019-10-29
US11130944B2 (en) 2021-09-28
KR20210072147A (ko) 2021-06-16
KR102265148B1 (ko) 2021-06-15
MX356997B (es) 2018-06-22
AU2017200596B2 (en) 2019-01-24
EP3385375B1 (en) 2021-06-16
ES2675224T3 (es) 2018-07-09
JP6523393B2 (ja) 2019-05-29
KR20170066694A (ko) 2017-06-14
KR102082247B1 (ko) 2020-02-28
JP6926143B2 (ja) 2021-08-25
CA2898317A1 (en) 2014-07-24
MY167434A (en) 2018-08-28
US20220090028A1 (en) 2022-03-24
MX2015009138A (es) 2015-11-06
WO2014113571A3 (en) 2014-11-13
AU2017200596A1 (en) 2017-02-23
EP3385375A1 (en) 2018-10-10
US20240117324A1 (en) 2024-04-11

Similar Documents

Publication Publication Date Title
JP7094343B2 (ja) 改良された特性を有するアシル－ａｃｐレダクターゼ
JP6738852B2 (ja) 改良された脂肪酸誘導体産生方法
JP6230594B2 (ja) Ｃａｒ酵素、および改良された脂肪アルコール産生方法
JP2016500261A (ja) 脂肪酸誘導体のａｃｐ媒介性生産方法
JP7564800B2 (ja) 特性が改良されたオメガ－ヒドロキシラーゼ関連融合ポリペプチド
JP7458358B2 (ja) 改良された特性を有するω－ヒドロキシラーゼ関連融合ポリペプチドバリアント
JP6486372B2 (ja) 改善されたアセチル−ＣｏＡカルボキシラーゼ変種

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150917

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160808

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160808

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20160808

A975 Report on accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A971005

Effective date: 20160906

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160914

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20161014

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20161205

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20161216

R150 Certificate of patent or registration of utility model

Ref document number: 6063585

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250