CN105012551A - Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract - Google Patents
Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract Download PDFInfo
- Publication number
- CN105012551A CN105012551A CN201510460189.7A CN201510460189A CN105012551A CN 105012551 A CN105012551 A CN 105012551A CN 201510460189 A CN201510460189 A CN 201510460189A CN 105012551 A CN105012551 A CN 105012551A
- Authority
- CN
- China
- Prior art keywords
- ethanol
- sanye
- radix
- preparation
- folium isatidis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an ethanol extract of radix tetrastigmae leaves as well as a preparation method and application of the ethanol extract. The preparation method comprises the steps of smashing dried radix tetrastigmae leaves to obtain dry powder; extracting by using an ethanol water solution, and filtering to obtain filtrate; and drying the filtrate to obtain the ethanol extract of the radix tetrastigmae leaves. According to the preparation method, bacterium inhibiting and killing components in the radix tetrastigmae leaves are extracted through extracting by using the ethanol water solution, and finally, the ethanol extract of the radix tetrastigmae leaves is obtained. The ethanol extract of the radix tetrastigmae leaves, prepared by using the preparation method, can be used for preparing bacterium inhibiting and killing supplies, can be used for inhibiting and killing bacteria on drugs, cosmetics, health foods and foods as well as bacteria generated in human, animals and aquaculture, and can be used for effectively inhibiting bacterial growth and killing bacteria.
Description
Technical field
The present invention relates to the technical field of Radix Apioris Fortunei (Radix Lespedezae Buergeri) leaf extract, be specifically related to ethanol extraction of a kind of SANYE Folium Isatidis and its preparation method and application.
Background technology
Chinese medicine Radix Apioris Fortunei (Radix Lespedezae Buergeri) (Tetrastigma hemsleyanum Diels et Gilg) is Vitaceae Tetrastigma tetratigma hemsleyanum, be born in the sylvan life that dark and damp hillside, mountain valley or small stream are other, be mainly distributed in the provinces and regions such as Southwestern China and Zhejiang, Anhui, Jiangxi, Fujian, Guangxi.Radix Apioris Fortunei (Radix Lespedezae Buergeri) is mainly with tuber, and also useful all herbal medicine, but without special instruction, the Radix Apioris Fortunei (Radix Lespedezae Buergeri) that current pharmacy is sold and commercially available Radix Apioris Fortunei (Radix Lespedezae Buergeri) prepared slices of Chinese crude drugs powder are Radix Apioris Fortunei (Radix Lespedezae Buergeri) tuber position, and the Radix Apioris Fortunei (Radix Lespedezae Buergeri) in pharmacopeia and document also refers to the tuber position of Radix Apioris Fortunei (Radix Lespedezae Buergeri) mostly.The tuber of Radix Apioris Fortunei (Radix Lespedezae Buergeri) has effects such as improving immunity, antiviral, enhancing liver function, especially to hepatitis B, kinds of tumors, cancer, children with high fever induced convulsions, bronchitis, pneumonia, pharyngolaryngitis, viral encephalitis etc., there is unique curative effect, be described as superfine product Chinese herbal medicine by Chinese medicine circle.The fresh medicine price of Radix Apioris Fortunei (Radix Lespedezae Buergeri) tuber up to every kilogram 2000 yuan in the market, the market price of prepared slices of Chinese crude drugs Radix Apioris Fortunei (Radix Lespedezae Buergeri) tuber powder reaches 10 yuan every gram, and supply falls short of demand.
The market demand of Radix Apioris Fortunei (Radix Lespedezae Buergeri) tuber is vigorous, and the rattan of Radix Apioris Fortunei (Radix Lespedezae Buergeri) is relative with the pharmaceutical research at the position of leaf less, and market demand is also little, and the rattan of most Radix Apioris Fortunei (Radix Lespedezae Buergeri) and leaf are abandoned by as waste material.Therefore the application on pharmacology for active development identification of lligera rhodantha hance and leaf, effectively can improve the comprehensive utilization at each position of Radix Apioris Fortunei (Radix Lespedezae Buergeri), increase economic efficiency.
Radix Apioris Fortunei (Radix Lespedezae Buergeri) has unique curative effect, the good reputation have " bouvardin " among the people because of the high heat to unknown cause, children with high fever induced convulsions, all kinds of inflammatory edema.But what existing pharmacopeia and document were recorded all bring down a fever for Radix Apioris Fortunei (Radix Lespedezae Buergeri) and the function of antiinflammatory, directly can have no record with the effect of killing antibacterial by bacteria growing inhibiting for Radix Apioris Fortunei (Radix Lespedezae Buergeri), particularly Radix Apioris Fortunei (Radix Lespedezae Buergeri) bacteria growing inhibiting has no report with the active component of the effect of killing antibacterial.
Publication number is that the Chinese invention patent application of CN 101417000A (application number is 200810120750.7) discloses a kind of new method preparing Lemna paucicostata, the i.e. method of high efficiency extraction separating flavone compounds from Radix Apioris Fortunei (Radix Lespedezae Buergeri), Radix Apioris Fortunei (Radix Lespedezae Buergeri) is dry, pulverize, add water to extract in ultrasonic extractor, extracting solution is concentrated into certain volume, adsorption bleaching is carried out again with active carbon, then cavitation suspension extraction segregation apparatus is utilized, first be extracted with ethyl acetate, reclaim ethyl acetate, by aqueous solution part chloroform extraction, reclaim chloroform, by aqueous solution partial concentration extremely without organic reagent taste, pass through macroporous adsorbent resin, use water and 50% ethanol elution successively, collect 50% ethanol elution, recycling design, obtain Lemna paucicostata.This technical scheme by ethyl acetate and chloroform extraction, high efficiency extraction separating flavone compounds, but cannot know the purposes of the flavone compound of its extraction and isolation.
Application publication number is that the Chinese invention patent application of CN 103381222A (application number is 201210144289.5) discloses kind of plant Radix Apioris Fortunei (Radix Lespedezae Buergeri) gaseous formulation and a preparation method, wherein, Radix Apioris Fortunei (Radix Lespedezae Buergeri) original liquid component extracting method comprises: Radix Apioris Fortunei (Radix Lespedezae Buergeri) former medicine 1Kg ethanol 50%3 ~ 4 times leaching twice, mixed by the supernatant of extracted twice, it is for subsequent use that 1:1 stock solution made by recovery ethanol.This technical scheme makes spray by the component of following weight percentage, and formulation components is as follows: Radix Apioris Fortunei (Radix Lespedezae Buergeri) stock solution 10% ~ 30%, polyvidone 3% ~ 6%, poloxamer 0.3% ~ 5%, glycerol 1% ~ 3%, ethanol 2% ~ 6%, Mentholum 0.02% ~ 0.05%, xylitol 2% ~ 4%, purified water surplus.This spray can be used for antiviral, analgesia detumescence by said components composite, can specifically for treating the upper respiratory tract infection of feeling the mucosa part such as wind and cold, oral cavity and being used for the treatment of hemorrhoid abscess outward.But how by extracting solution, extract the Radix Apioris Fortunei (Radix Lespedezae Buergeri) stock solution that antibiotic property is best, this patent application specification is also unexposed.
Summary of the invention
The invention provides ethanol extraction of a kind of SANYE Folium Isatidis and its preparation method and application, this ethanol extraction can effective bacteria growing inhibiting and kill antibacterial.
A preparation method for the ethanol extraction of SANYE Folium Isatidis, comprises the following steps:
The leaf pulverizing of getting dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) obtains dry powder, and extract with ethanol water, filter, filtrate obtains the ethanol extraction of SANYE Folium Isatidis after drying.
In the present invention, extracted by ethanol water, antibacterial and sterilization component in the leaf of Radix Apioris Fortunei (Radix Lespedezae Buergeri) extracted, finally obtains the ethanol extraction of SANYE Folium Isatidis, the ethanol extraction of the SANYE Folium Isatidis obtained can effectively bacteria growing inhibiting with kill antibacterial.
As preferably, in described ethanol water, the percentage by volume of ethanol is 70% ~ 95%, extracts 60min ~ 120min with ethanol water.Antibacterial in SANYE Folium Isatidis and sterilization component can be extracted better under above-mentioned condition.Further preferably, in described ethanol water, the percentage by volume of ethanol is 95%, 90min is extracted with ethanol water, the polarity of the ethanol water of above-mentioned concentration is applicable to antibacterial in the leaf of Radix Apioris Fortunei (Radix Lespedezae Buergeri) and sterilization component to extract very much, and above-mentioned condition is conducive to extraction that is antibacterial in the leaf of Radix Apioris Fortunei (Radix Lespedezae Buergeri) and sterilization component.The consumption of described ethanol water is the 5-50mL/g of dry powder quality.Namely the described quality of dry powder is 1g:5-50mL with the ratio of the volume of ethanol water, and namely every gram of dry powder adds the ethanol water of 5-50mL.Further preferably, the consumption of described ethanol water is the 15mL/g of dry powder quality, and the namely described quality of dry powder is 1g:15mL with the ratio of the volume of ethanol water.
The ethanol extraction of SANYE Folium Isatidis prepared by described preparation method, can be used for preparing bacteriostasis and sterilization articles for use, for medicine, cosmetics, health food, food and antibacterial and antibacterial in people, animal, aquaculture process.
A preparation method for antibacterial medicinal liquid, comprises the following steps:
The ethanol extraction of SANYE Folium Isatidis is dissolved in the water containing lytic agent, after the membrane filtration of 0.2 ~ 0.5 μm is degerming, obtained antibacterial medicinal liquid;
The quality of the ethanol extraction of described SANYE Folium Isatidis is 1-16mg:1mL with the ratio of the volume of the water containing lytic agent;
Described lytic agent is dimethyl sulfoxide (DMSO) or ethanol, and described is 0.1% ~ 2% containing the percentage by volume of lytic agent in the water of lytic agent.The present invention adopts specific lytic agent, can antibacterial in the ethanol extraction of SANYE Folium Isatidis and sterilization component be dissolved in the water containing lytic agent well, form stable solution, and through the filter membrane removing impurity of 0.2 ~ 0.5 μm, impurity is mainly the impurity that can not dissolve (without antibiotic effect).The present invention adopts the specific lytic agent of certain concentration, antibacterial and sterilization component is retained in antibacterial medicinal liquid to greatest extent, and the antibacterial medicinal liquid that the present invention is obtained is to bacteria growing inhibiting and kill antibacterial and have and act on preferably.Further preferably, described is 2% containing the percentage by volume of lytic agent in the water of lytic agent.
Described antibacterial medicinal liquid can be used for preparing bacteriostasis and sterilization articles for use, for medicine, cosmetics, health food, food and antibacterial and antibacterial in people, animal, aquaculture process.
Compared with prior art, tool of the present invention has the following advantages:
In the present invention, extracted by ethanol water, antibacterial and sterilization component in the leaf of Radix Apioris Fortunei (Radix Lespedezae Buergeri) extracted, finally obtains the ethanol extraction of SANYE Folium Isatidis, the ethanol extraction of the SANYE Folium Isatidis obtained can effectively bacteria growing inhibiting with kill antibacterial.
The ethanol extraction of SANYE Folium Isatidis prepared by the inventive method has antibiotic and sterilizing effect, can be used for preparing bacteriostasis and sterilization articles for use.As made antibacterial medicinal liquid, the present invention adopts the specific lytic agent of certain concentration, antibacterial and sterilization component is retained in antibacterial medicinal liquid to greatest extent, and the antibacterial medicinal liquid that the present invention is obtained is to bacteria growing inhibiting and kill antibacterial and have and act on preferably.This medicinal liquid can be used as composition for medicine, cosmetics, health food, food and antibacterial and antibacterial in people, animal, aquaculture process.The present invention is the purposes of new medicine, cosmetics, health food and food outside the original open effect of Radix Apioris Fortunei (Radix Lespedezae Buergeri), has bacteriostasis antibiosis effect, possesses wide application prospect.
Detailed description of the invention
Embodiment 1
(1) the leaf pulverizing of getting dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) obtains dry powder, 90min is extracted with the ethanol water of percentage by volume 95%, the quality of dry powder is 1g:15mL with the ratio of the volume of ethanol water, filters, and filtrate drying obtains the ethanol extraction of SANYE Folium Isatidis;
(2) ethanol extraction of SANYE Folium Isatidis is dissolved in the water containing the DMSO (dimethyl sulfoxide) of percentage by volume 2% with the concentration of 16mg/mL, after the membrane filtration of 0.45 μm is degerming, obtained antibacterial medicinal liquid, each medicinal liquid is for subsequent use under 4 DEG C of conditions.
(3) minimal inhibitory concentration (MIC) of each medicinal liquid to antibacterial is detected.Namely antibacterial in LB culture medium under 37 DEG C of conditions incubated overnight, 1:15 is transferred in new LB culture medium and continues to cultivate to OD
620=0.2 ± 0.02.Antibacterial 1:10000 is diluted in LB culture medium, adds isopyknic medicinal liquid, and the mixture getting same volume after mixing joins next dilution factor, and two-fold dilution is to last concentration always.Negative control adds isopyknic water containing percentage by volume 2%DMSO.
(4) antibacterial cultivates 18-20 hour under 37 DEG C of conditions, reads OD
620, OD
620being less than for 0.1 (being namely less than background numerical value) is judged to be that, without bacterial growth, result of the test is as shown in table 1.
Table 1
The above results shows, when the concentration of the ethanol extraction of SANYE Folium Isatidis is more than or equal to 0.5mg/mL, can suppress the growth of staphylococcus aureus ATCC6538; When concentration is more than or equal to 0.5mg/mL, the growth of listerisa monocytogenes in mjme NCTC7973 can be suppressed; When concentration is more than or equal to 1mg/mL, the growth of escherichia coli ATCC8739 can be suppressed.These results suggest that the ethanol extraction of SANYE Folium Isatidis has good inhibitory action to antibacterial, there is bacteriostasis and sterilization effect.
Embodiment 2
(1) the leaf pulverizing of getting dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) obtains dry powder, 90min is extracted with the ethanol water of percentage by volume 70%, the quality of dry powder is 1g:15mL with the ratio of the volume of ethanol water, filters, and filtrate drying obtains the ethanol extraction of SANYE Folium Isatidis;
(2) ethanol extraction of SANYE Folium Isatidis is dissolved in the water containing percentage by volume 2% ethanol with the concentration of 16mg/mL, and after the membrane filtration of 0.45 μm is degerming, obtained antibacterial medicinal liquid, each medicinal liquid is for subsequent use under 4 DEG C of conditions.
(3) minimal inhibitory concentration (MIC) of each medicinal liquid to antibacterial is detected.Namely antibacterial in LB culture medium under 37 DEG C of conditions incubated overnight, 1:15 is transferred in new LB culture medium and continues to cultivate to OD
620=0.2 ± 0.02.Antibacterial 1:10000 is diluted in LB culture medium, adds isopyknic medicinal liquid, and the mixture getting same volume after mixing joins next dilution factor, and two-fold dilution is to last concentration always.Negative control adds isopyknic water containing percentage by volume 2% ethanol.
(4) antibacterial cultivates 18-20 hour under 37 DEG C of conditions, reads OD
620, OD
620being less than for 0.1 (being namely less than background numerical value) is judged to be without bacterial growth.Result of the test is as shown in table 2.
Table 2
The above results shows, when the concentration of the ethanol extraction of SANYE Folium Isatidis is more than or equal to 0.5mg/mL, can suppress the growth of staphylococcus aureus ATCC6538; When concentration is more than or equal to 1mg/mL, the growth of listerisa monocytogenes in mjme NCTC7973 can be suppressed; When concentration is more than or equal to 1mg/mL, the growth of escherichia coli ATCC8739 can be suppressed.These results suggest that the ethanol extraction ethyl acetate extract of SANYE Folium Isatidis has good inhibitory action to antibacterial, there is bacteriostasis and sterilization effect.
Comparative example 1
The root pulverizing of getting dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) obtains dry powder, 90min is extracted with the ethanol water of percentage by volume 50%, 75%, 95%, the quality of dry powder is 1g:3mL with the ratio of the volume of ethanol water, filter, filtrate drying obtains the extract (i.e. the ethanol extract of SANYE QINGGEN) of Radix Apioris Fortunei (Radix Lespedezae Buergeri), adds water and is mixed with the antibacterial medicinal liquid of 16mg/mL concentration.Result of the test is as shown in table 3, and wherein, the water extract of SANYE QINGGEN refers to that Extraction solvent water replaces ethanol water, and other are constant.
Table 3
The above results shows, the water extract of the root of the Radix Apioris Fortunei (Radix Lespedezae Buergeri) of 16mg/mL and ethanol extract are to staphylococcus aureus ATCC6538, listerisa monocytogenes in mjme NCTC7973, escherichia coli ATCC8739, Salmonella enteritidis CGMCG50760, the equal unrestraint effect of Pseudomonas aeruginosa ATCC9027, vibrio parahaemolytious ATCC17082, Salmonella typhimurium ATCC14028.Compare with 2 results with table 1, the water extract of SANYE QINGGEN and the fungistatic effect of ethanol extract will be starkly lower than the ethanol extract of SANYE Folium Isatidis.
Claims (9)
1. a preparation method for the ethanol extraction of SANYE Folium Isatidis, is characterized in that, comprises the following steps:
The leaf pulverizing of getting dry Radix Apioris Fortunei (Radix Lespedezae Buergeri) obtains dry powder, and extract with ethanol water, filter, filtrate obtains the ethanol extraction of SANYE Folium Isatidis after drying.
2. the preparation method of the ethanol extraction of SANYE Folium Isatidis according to claim 1, is characterized in that, in described ethanol water, the percentage by volume of ethanol is 70% ~ 95%, extracts 60min ~ 120min with ethanol water.
3. the preparation method of the ethanol extraction of SANYE Folium Isatidis according to claim 2, is characterized in that, in described ethanol water, the percentage by volume of ethanol is 95%, extracts 90min with ethanol water.
4. the preparation method of the ethanol extraction of SANYE Folium Isatidis according to claim 1, is characterized in that, the consumption of described ethanol water is the 5-50mL/g of dry powder quality.
5. the preparation method of the ethanol extraction of SANYE Folium Isatidis according to claim 4, is characterized in that, the consumption of described ethanol water is the 15mL/g of dry powder quality.
6. the ethanol extraction of SANYE Folium Isatidis prepared by the preparation method according to any one of Claims 1 to 5.
7. the ethanol extraction of SANYE Folium Isatidis according to claim 6 is in the application preparing bacteriostasis and sterilization articles for use.
8. a preparation method for antibacterial medicinal liquid, is characterized in that, comprises the following steps:
The ethanol extraction of SANYE Folium Isatidis is dissolved in the water containing lytic agent, after the membrane filtration of 0.2 ~ 0.5 μm is degerming, obtained antibacterial medicinal liquid;
The quality of the ethanol extraction of described SANYE Folium Isatidis is 1-16mg:1mL with the ratio of the volume of the water containing lytic agent;
Described lytic agent is dimethyl sulfoxide or ethanol, and described is 0.1% ~ 2% containing the percentage by volume of lytic agent in the water of lytic agent.
9. antibacterial medicinal liquid is preparing the application in bacteriostasis and sterilization articles for use, it is characterized in that, described antibacterial medicinal liquid adopts preparation method according to claim 8 to prepare.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510460189.7A CN105012551A (en) | 2015-07-30 | 2015-07-30 | Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510460189.7A CN105012551A (en) | 2015-07-30 | 2015-07-30 | Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105012551A true CN105012551A (en) | 2015-11-04 |
Family
ID=54403096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510460189.7A Pending CN105012551A (en) | 2015-07-30 | 2015-07-30 | Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105012551A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105878124A (en) * | 2016-06-03 | 2016-08-24 | 宁波圣旺生物科技有限公司 | Radix tetrastigme toothpaste and preparation method thereof |
| CN106281797A (en) * | 2016-08-09 | 2017-01-04 | 宁波圣旺生物科技有限公司 | Radix Apioris Fortunei (Radix Lespedezae Buergeri) soap and preparation method thereof |
| CN106389188A (en) * | 2016-07-21 | 2017-02-15 | 上海蔚艺生物科技有限公司 | Chinese herbal medicine toothpaste containing radix tetrastigme and rhizoma bletillae |
| CN106417914A (en) * | 2016-10-10 | 2017-02-22 | 广州市义和化工有限公司 | Feed additive capable of taking place of antibiotic and preparation and use method of feed additive |
| CN107519328A (en) * | 2017-09-05 | 2017-12-29 | 浙江豆豆宝中药研究有限公司 | A kind of radix tetrastigme antimicrobial fluid and preparation method thereof |
| CN108888685A (en) * | 2018-07-26 | 2018-11-27 | 杭州金域医学检验所有限公司 | Purposes and its action method of the position Lemna paucicostata TH-w to staphylococcus aureus |
| CN112535699A (en) * | 2020-12-14 | 2021-03-23 | 浙江理工大学绍兴生物医药研究院有限公司 | Compound additive for preventing and treating recessive mastitis of dairy animals and application thereof |
| CN113576982A (en) * | 2021-09-02 | 2021-11-02 | 和也健康科技有限公司 | Preparation method of multifunctional nano stone needle fiber mask capable of diminishing inflammation and whitening skin |
| CN114452242A (en) * | 2022-03-23 | 2022-05-10 | 朱建国 | Beauty mask with radix tetrastigme as main material |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104435401A (en) * | 2014-11-13 | 2015-03-25 | 福建中医药大学 | Method for purifying general flavone from aboveground part of radix tetrastigme |
-
2015
- 2015-07-30 CN CN201510460189.7A patent/CN105012551A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104435401A (en) * | 2014-11-13 | 2015-03-25 | 福建中医药大学 | Method for purifying general flavone from aboveground part of radix tetrastigme |
Non-Patent Citations (2)
| Title |
|---|
| 国家食品药品监督管理局药品认证管理中心编写: "《无菌药品》", 31 August 2011, 中国医药科技出版社 * |
| 张晶: "《中药化学》", 30 April 2015, 中国农业大学出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105878124A (en) * | 2016-06-03 | 2016-08-24 | 宁波圣旺生物科技有限公司 | Radix tetrastigme toothpaste and preparation method thereof |
| CN106389188A (en) * | 2016-07-21 | 2017-02-15 | 上海蔚艺生物科技有限公司 | Chinese herbal medicine toothpaste containing radix tetrastigme and rhizoma bletillae |
| CN106281797A (en) * | 2016-08-09 | 2017-01-04 | 宁波圣旺生物科技有限公司 | Radix Apioris Fortunei (Radix Lespedezae Buergeri) soap and preparation method thereof |
| CN106417914A (en) * | 2016-10-10 | 2017-02-22 | 广州市义和化工有限公司 | Feed additive capable of taking place of antibiotic and preparation and use method of feed additive |
| CN107519328A (en) * | 2017-09-05 | 2017-12-29 | 浙江豆豆宝中药研究有限公司 | A kind of radix tetrastigme antimicrobial fluid and preparation method thereof |
| CN108888685A (en) * | 2018-07-26 | 2018-11-27 | 杭州金域医学检验所有限公司 | Purposes and its action method of the position Lemna paucicostata TH-w to staphylococcus aureus |
| CN108888685B (en) * | 2018-07-26 | 2021-12-10 | 杭州金域医学检验所有限公司 | Application of Tetrastigma hemsleyanum Diels et Gilg extract TH-w part to staphylococcus aureus and action method thereof |
| CN112535699A (en) * | 2020-12-14 | 2021-03-23 | 浙江理工大学绍兴生物医药研究院有限公司 | Compound additive for preventing and treating recessive mastitis of dairy animals and application thereof |
| CN113576982A (en) * | 2021-09-02 | 2021-11-02 | 和也健康科技有限公司 | Preparation method of multifunctional nano stone needle fiber mask capable of diminishing inflammation and whitening skin |
| CN114452242A (en) * | 2022-03-23 | 2022-05-10 | 朱建国 | Beauty mask with radix tetrastigme as main material |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105012551A (en) | Ethanol extract of radix tetrastigmae leaves as well as preparation method and application of ethanol extract | |
| CN105061616B (en) | Identification of lligera rhodantha hance polyoses extract and its preparation method and application | |
| CN105379771A (en) | Plant-derived pesticide and its preparation method and use | |
| CN105519613A (en) | Novel botanical insecticide, preparation method and application thereof | |
| CN105594765A (en) | Environment-friendly pesticide | |
| CN105532767A (en) | Insecticide, preparation method and application thereof | |
| CN105519612A (en) | Botanical insecticide for fruit trees, preparation method and application thereof | |
| CN107501065B (en) | Polysubstituted naphthalene compound with antibacterial activity in aloe and preparation method and application thereof | |
| CN108338406A (en) | A kind of quick-fried pearl of rosemary antioxidant and its application in cigarette filter rod | |
| CN102870995B (en) | Chinese herbal medicine additive for pig feed | |
| Abkhoo et al. | Antibacterial effects of aqueous and ethanolic extracts of medicinal plants against pathogenic strains | |
| CN105379780A (en) | Pesticide, as well as preparation method and application thereof | |
| CN105284942A (en) | Novel botanical insecticide, preparation method and application of novel botanical insecticide | |
| CN103385283B (en) | Preparation method for antiseptic insect-proof antistaling agent used for bacon | |
| Chaturvedi et al. | Formulation and evaluation of polyherbal anthelmintic preparation | |
| CN105248469A (en) | Plant source pesticide, preparation method and application thereof | |
| CN101773297B (en) | Cypress essential oil and cigarette containing same | |
| KR101283775B1 (en) | Pharmaceuticlal composition for anti-tussive activity or discharge of phlegm activity comprising extracts of dendropanax morbifera | |
| CN104522339A (en) | Fish feed additive used for controlling and preventing skin diseases, and preparation method thereof | |
| CN113303493A (en) | Plant-derived bacteriostatic essential oil for electronic atomization rod and preparation method thereof | |
| CN105055682A (en) | Ethanol extract ethyl acetate extractive fraction of Tetrastigma hemsleyanum leaf and preparing method and application thereof | |
| CN105248470A (en) | Plant source fruit tree pesticide, preparation method and application thereof | |
| CN101755859A (en) | Traditional Chinese medicine composition for preventing parasite in water, preparation method and application thereof | |
| RU2303992C1 (en) | Curative-prophylactic remedy for youngsters of farm animals and method for preventing and treating respiratory diseases in calves due to its application | |
| CN103446249B (en) | A kind of preparation technology of hepatic cholagogic granule |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151104 |