CN104903465B - 基于寡核苷酸切割及延伸‑依赖性非‑杂交探测的靶核酸序列的检测 - Google Patents
基于寡核苷酸切割及延伸‑依赖性非‑杂交探测的靶核酸序列的检测 Download PDFInfo
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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| KR20120154834 | 2012-12-27 | ||
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| KR10-2013-0034670 | 2013-03-29 | ||
| KR20130034670 | 2013-03-29 | ||
| PCT/KR2013/012312 WO2014104818A1 (en) | 2012-12-27 | 2013-12-27 | Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay |
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| CN104903465A CN104903465A (zh) | 2015-09-09 |
| CN104903465B true CN104903465B (zh) | 2017-04-26 |
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| EP3469100B1 (en) | 2016-06-10 | 2021-01-27 | Seegene, Inc. | Methods for preparing tagging oligonucleotides |
| KR102301305B1 (ko) | 2016-06-30 | 2021-09-13 | 주식회사 씨젠 | 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기 |
| WO2018066950A1 (en) | 2016-10-06 | 2018-04-12 | Seegene, Inc. . | Methods for preparing oligonucleotides for detecting target nucleic acid molecules in samples |
| EP3559275A1 (de) * | 2016-12-23 | 2019-10-30 | Albert-Ludwigs-Universität Freiburg | Zweiteilige mediatorsonde |
| ES2972231T3 (es) | 2016-12-29 | 2024-06-11 | Seegene Inc | Procedimiento para reducir la formación de dímeros de cebadores y aumentar la eficacia de la amplificación |
| WO2018182281A1 (en) | 2017-03-28 | 2018-10-04 | Seegene, Inc. . | Analytical signal for determination of the presence of a target nucleic acid sequence |
| CN108823287B (zh) | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
| CN107236815A (zh) * | 2017-07-18 | 2017-10-10 | 江西贤聚景欣医药生物科技有限公司 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
| WO2019045532A2 (en) | 2017-08-31 | 2019-03-07 | Seegene, Inc. | EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS |
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| US12391997B2 (en) | 2018-01-05 | 2025-08-19 | Seegene, Inc. | Method for determining the presence or absence of M. tuberculosis, M. bovis and M. bovis BCG in a sample |
| KR102464501B1 (ko) | 2018-04-20 | 2022-11-17 | 주식회사 씨젠 | 샘플 내 복수의 타겟 핵산 서열의 검출을 위한 방법 및 장치 |
| WO2020122679A1 (en) | 2018-12-14 | 2020-06-18 | Seegene, Inc. | Method for detecting a target analyte in a sample using an s-shaped function for a slope data set |
| US20220298555A1 (en) * | 2018-12-20 | 2022-09-22 | Roche Molecular Systems, Inc. | Detection of target nucleic acid by solid-phase molography |
| WO2023043280A1 (ko) | 2021-09-17 | 2023-03-23 | 주식회사 씨젠 | 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출 |
| EP4511509A1 (de) * | 2022-04-22 | 2025-02-26 | Hahn-Schickard-Gesellschaft für angewandte Forschung e. V. | Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes |
| EP4265734A1 (de) * | 2022-04-22 | 2023-10-25 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes |
| KR20250086619A (ko) | 2022-10-14 | 2025-06-13 | 주식회사 씨젠 | 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법 |
| EP4372100A1 (en) * | 2022-11-15 | 2024-05-22 | Stilla Technologies | Data analysis method for multiplex digital pcr using color-combination |
| WO2024106890A1 (ko) | 2022-11-16 | 2024-05-23 | 주식회사 씨젠 | 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension; q-ptoce) 어세이에 의한 타겟 핵산의 검출 |
| WO2025005717A1 (ko) * | 2023-06-30 | 2025-01-02 | 주식회사 씨젠 | Hto 절단 및 자기-연장 어세이에 의해 샘플 내의 타겟 핵산을 검출하는 방법 |
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- 2013-12-27 AU AU2013371128A patent/AU2013371128B2/en active Active
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- 2013-12-27 WO PCT/KR2013/012312 patent/WO2014104818A1/en active Application Filing
- 2013-12-27 EP EP13868457.6A patent/EP2943586B1/en active Active
- 2013-12-27 KR KR1020157017974A patent/KR101760267B1/ko active Active
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- 2013-12-27 RU RU2015130335A patent/RU2620958C2/ru active
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Also Published As
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|---|---|
| JP6178430B2 (ja) | 2017-08-09 |
| CA2896213A1 (en) | 2014-07-03 |
| AU2013371128B2 (en) | 2017-08-10 |
| KR101760267B1 (ko) | 2017-07-24 |
| KR20150093766A (ko) | 2015-08-18 |
| ES2653642T3 (es) | 2018-02-08 |
| EP2943586B1 (en) | 2017-09-20 |
| EP2943586A1 (en) | 2015-11-18 |
| WO2014104818A1 (en) | 2014-07-03 |
| AU2013371128A1 (en) | 2015-08-13 |
| CN104903465A (zh) | 2015-09-09 |
| US9783845B2 (en) | 2017-10-10 |
| US20180057869A1 (en) | 2018-03-01 |
| US20160060690A1 (en) | 2016-03-03 |
| EP2943586A4 (en) | 2016-09-28 |
| MX361191B (es) | 2018-11-29 |
| RU2015130335A (ru) | 2017-01-30 |
| CA2896213C (en) | 2018-12-11 |
| MX2015008494A (es) | 2015-09-10 |
| RU2620958C2 (ru) | 2017-05-30 |
| BR112015014152B1 (pt) | 2023-01-24 |
| BR112015014152A2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2017-08-22 |
| JP2016502861A (ja) | 2016-02-01 |
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