CN104857359A

CN104857359A - Medicinal composition for treating piglet dysentery and preparation method of medicinal composition

Info

Publication number: CN104857359A
Application number: CN201510318181.7A
Authority: CN
Inventors: 秦立廷; 李鑫; 黄河
Original assignee: Shandong New Hope Liuhe Group Co Ltd
Current assignee: Shandong New Hope Liuhe Group Co Ltd
Priority date: 2015-06-11
Filing date: 2015-06-11
Publication date: 2015-08-26

Abstract

The invention discloses a medicinal composition for treating piglet dysentery and a preparation method of the medicinal composition. The medicinal composition comprises the following raw medicine materials: whorlleaf loosestrife herb, lepisorus waltonii ching, cirsium souliei, semen iris lacteae, codonopsis pilosula, radix chloranthi serrate, garlic, semen cuscutae, hovenia acerba, rheum officinale, antenoron root, the root of Chinese pulsatilla, purslane, garden burnet, cimicifugae foetidae, radix tinosporae, semen euryales, hemsleya sphaerocarpa, folium isatidis, sophora flower, potentilla plumose, areca nut, smilax hypoglauca, violet, cortex moutan, vervain, herba pteridis multifidae, panicum repens, herba violae, indigo naturalis, lalang grass rhizome, lycianthes biflora and balanophora harlandii. The medicinal composition for treating piglet dysentery has the functions of clearing heat and removing toxicity, cooling blood and relieving dysentery, can be mainly used for treating the dysentery of piglets which are 2-3 months old, can also be used for improving the immunity of the piglets and enhancing the disease resistance, and has the advantages of quick curative effect, short course of treatment and no toxic and side effects during treatment on piglet dysentery.

Description

A kind of pharmaceutical composition for the treatment of piglet diarrhea and preparation method thereof

Technical field

The present invention relates to field of veterinary, particularly a kind of pharmaceutical composition for the treatment of piglet diarrhea and preparation method thereof.

Background technology

Swine dysentery is again Sanguis sus domestica dysentery, and be a kind of serious infectious intestinal disease caused by swine dysentery Serpulina, swine dysentery Serpulina is that Serpulina belongs to member, incorporates treponema in the past.Thalline is long 6 ~ 8.5 microns, wide 0.3 ~ 0.37 micron, mostly is 4 ~ 6 and bends, and 5 ~ 6 that have bend, and two ends are sharp-pointed, shape as two wild goose wing, Gram-negative.The spirochete motility that fresh pathological material of disease is active as seen under darkfield microscope.Cause of disease to external world environment has stronger resistance, can survive 61 days in feces when 5 DEG C, can survive 7 days for 25 DEG C, can survive 102 days for 4 DEG C in soil.But not strong to disinfectant resistance, it all can kill rapidly by common disinfectant.The main clinical symptom of swine dysentery is serious mucus hemorrhagic dysentery, and acute is based on hemorrhagic dysentery, subacute and chronic based on mucous diarrhea.Cuing open inspection pathological characters is colorectal mucosa generation Catarrhal, hemorrhagic and gangrenous inflammation.Clinical manifestation for becoming thin, mucus or hemorrhagic dysentery.

Swine dysentery characteristics of incidence: in natural situation, only has pig to fall ill, and the pig of various age, kind all can infect, but main infringement is the piglet at 2-3 monthly age; The M & M of piglets is all high than large pig; Sick pig and bacillicarrier are main source of infection, and the generation of primary disease is without obvious seasonality; Due to the existence of the pig that carries disease germs, transfer frequently by swinery and buy and sell pig and disease is scattered.Carry disease germs pig, often do not fall ill under normal feeding and management condition, when have reduce pig body resistance unfavorable factor, raise not enough, be deficient in vitamin and stressors time, just can promote to cause morbidity.

Clinical symptom: swine dysentery incubation period is 2 days to about 3 months, and average out to 1 ~ 2 week, modal symptom is the diarrhoea occurring that degree is different.To have loose bowels, feces is medicated porridge sample or water sample, and including mucus, mucosa or blood is feature.Body temperature is had to raise and stomachache phenomenon.The also performance that the course of disease is long is dewatered, is become thin and ataxia.Acute, subacute and chronic three types can be divided according to course of disease length.Initial stage of breaking out is many in acute, subsequently based on subacute and chronic, and the most acute sudden death.

Acute case: modal symptom is the diarrhoea occurring that degree is different.Be generally first stake excrement, fade to yellow loose stool, interior mixed mucus or band blood.Discharge feces when being in a bad way the pasty state that just takes on a red color, inside have a large amount of mucus, clot and purulent secretion, what have draws the even green pasty state excrement of Lycoperdon polymorphum Vitt, brown, sometimes with a lot of minute bubbles, and is mixed with mucus and the downright bad pseudomembrane of cellulosic.Sick pig lassitude, anorexia and happiness drinking-water, the back of a bow, dehydration, walking is waved, and abdominal part is crispaturaed, and stomachache, plays abdomen with hind leg, slightly random, matt by hair, becomes thin rapidly, later stage defecation incontinence.Perianal and root of the tail are stain by feces, stand up unable, extremely weak, finally dead.The sick temperature of pig body of major part is normal, 40 ~ 40.5 DEG C, but is no more than 41 DEG C.Acute disease temperature of pig body raises and reaches 40 ~ 40.5 DEG C, poor appetite.Diarrhoea, starts to arrange the yellow soft stool to Lycoperdon polymorphum Vitt, then the loose stool of row containing a large amount of mucus band blood streak, rear passage of watery stools, and the fragment containing blood, mucus and white stickiness fibrinous exudate.Sick pig hogback, presents colic symptoms sometimes.Sick pig dehydration, weak, become thin.Blood is sticky, and leukocyte count increases, neutrophil cell shift to left.Large enterorrhagia, serious generation anemia.

Subacute and chronic cases: condition of illness is comparatively light, dysentery, and containing more mucus and slough fragment in excrement, blood is less; Stadium is longer, progressive emaciation, growth retardation, dysplasia.Some cases can spontaneous remission, but can recur within a certain period of time, even death occurs.To death from excrement of having loose bowels, about through 7 ~ 10 days.The sick pig of minority is not felt any better through treatment, and the course of disease can reach more than 15 days.

Clinical diagnosis: can tentative diagnosis be made according to epidemic characteristic, clinical symptoms and pathological characters, carry out lab testing, lab testing commonly uses Microscopical Method For Detection, namely fresh excreta (being preferably the mucus of the band blood streak) is got a little, or get fritter and have the direct smear of the colorectal mucosa of obvious pathological changes, fix through flame after natural drying in atmosphere, with ammonium oxalate crystal violet liquid, Ji's nurse dyeing liquor dyeing 3 ~ 5 minutes, after smear washing is dried in the shade, examine under a microscope, can see that swine dysentery is crawled spirillum, or above-mentioned pathological material of disease 1 droplet is placed on microscope slide, dripping 1 normal saline, mixing, then cover slide, with dark-field microscopy (400 times), when finding that there is the thalline vivaciously moved in Serpentis sample, can make a definite diagnosis.

At present, Modern medical therapy swine dysentery is mainly according to putting prevention first: medicine mainly contains: (1) LIJUNJING: calculate by every kg body weight 5mg, oral, every day 2 times, serveing on 3 ~ 5 days is a course for the treatment of; Or with 0.5% LIJUNJING solution, calculate by every kg body weight 0.5mL, intramuscular injection, is used in conjunction 3 ~ 5 days.(2) diformazan nitroimidazole: add 0.25g in every premium on currency, for sick pig drink-service after dissolving, connects drink 5 days; Feedstuff per ton adds 100g and feeds, and can prevent.Or by every kg body weight 5 ~ 10mg, oral at twice, serve on 3 ~ 4 days.(3) neomycin: add 140g feed by feedstuff per ton, connects and feeds 3 ~ 5 days; Add 100g in feedstuff per ton, feed, for prevention, 20 days can be used in conjunction.(4) lincomycin: add 100g feed in feedstuff per ton, connects and feeds 21 days; Add 40g feed in feedstuff per ton, can prevent.Above-mentioned therapeutic scheme, medicine are all limited, do not reach desirable therapeutic effect, and treatment cost are higher, and long-time application can cause untoward reaction, and even death waits side effect.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of pharmaceutical composition for the treatment of piglet diarrhea, the various defects of Chinese medicine of the prior art and Western medicine can be overcome, there is effect of heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, cure mainly the piglet diarrhea at 2-3 monthly age, the immunity of pig can also be improved simultaneously, strengthen its resistance against diseases, in treatment swine dysentery, there is curative effect fast, treatment short treating period, the advantage such as have no side effect.

In order to realize foregoing invention object, the invention provides a kind of pharmaceutical composition for the treatment of piglet diarrhea, described pharmaceutical composition comprises following raw medicinal material: see blood live, halberd Ye Wawei, Cirsium souliei (Franch.) Mattf., alkali ground Chinese small iris, Radix Codonopsis, Radix Chloranthi Serrati, Bulbus Allii, Herba cuscutae, Semen Hoveniae (Fructus Hoveniae), Radix Et Rhizoma Rhei, bright and beautiful line boomerang, the Radix Pulsatillae, Herba Portulacae, Radix Sanguisorbae, Rhizoma Cimicifugae, Radix Tinosporae, Semen Euryales, Hemsleya sphaerocarpa Kuang et A. M. Lu, Folium Isatidis, Flos Sophorae, Herba Potentillae Chinensis, Mel tank, powder Rhizoma Smilacis Chinensis, Climbing Viloet Herb, Cortex Moutan, Herba Verbenae, Herba Pteridis Multifidae, Herba panici repentics, Herba Violae, Indigo Naturalis, Rhizoma Imperatae, ten calyx eggplant and Balaenoptera borealis Lessons.

In described pharmaceutical composition, the ratio of weight and number of each raw medicinal material is: see that blood lives 10-35 weight portion, halberd Ye Wawei 12-22 weight portion, Cirsium souliei (Franch.) Mattf. 10-30 weight portion, alkali ground Chinese small iris 11-31 weight portion, Radix Codonopsis 14-28 weight portion, Radix Chloranthi Serrati 15-35 weight portion, Bulbus Allii 15-25 weight portion, Herba cuscutae 13-33 weight portion, Semen Hoveniae (Fructus Hoveniae) 11-21 weight portion, Radix Et Rhizoma Rhei 12-26 weight portion, brocade line boomerang 14-36 weight portion, Radix Pulsatillae 11-33 weight portion, Herba Portulacae 10-30 weight portion, Radix Sanguisorbae 13-33 weight portion, Rhizoma Cimicifugae 10-20 weight portion, Radix Tinosporae 13-27 weight portion, Semen Euryales 14-34 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 11-32 weight portion, Folium Isatidis 10-30 weight portion, Flos Sophorae 12-36 weight portion, Herba Potentillae Chinensis 10-28 weight portion, Mel tank 14-36 weight portion, powder Rhizoma Smilacis Chinensis 10-20 weight portion, Climbing Viloet Herb 12-24 weight portion, Cortex Moutan 10-20 weight portion, Herba Verbenae 11-23 weight portion, Herba Pteridis Multifidae 12-32 weight portion, Herba panici repentics 15-35 weight portion, Herba Violae 12-24 weight portion, Indigo Naturalis 13-33 weight portion, Rhizoma Imperatae 12-31 weight portion, ten calyx eggplant 11-29 weight portions and Balaenoptera borealis Lesson 14-35 weight portion.

In described pharmaceutical composition, the ratio of weight and number of each raw medicinal material is: see that blood lives 33 weight portions, halberd Ye Wawei 20 weight portion, Cirsium souliei (Franch.) Mattf. 25 weight portion, alkali ground Chinese small iris 31 weight portion, Radix Codonopsis 18 weight portion, Radix Chloranthi Serrati 32 weight portion, Bulbus Allii 25 weight portion, Herba cuscutae 31 weight portion, Semen Hoveniae (Fructus Hoveniae) 21 weight portion, Radix Et Rhizoma Rhei 24 weight portion, brocade line boomerang 32 weight portion, the Radix Pulsatillae 31 weight portion, Herba Portulacae 28 weight portion, Radix Sanguisorbae 32 weight portion, Rhizoma Cimicifugae 16 weight portion, Radix Tinosporae 25 weight portion, Semen Euryales 32 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 30 weight portion, Folium Isatidis 30 weight portion, Flos Sophorae 34 weight portion, Herba Potentillae Chinensis 22 weight portion, Mel tank 32 weight portion, powder Rhizoma Smilacis Chinensis 20 weight portion, Climbing Viloet Herb 14 weight portion, Cortex Moutan 16 weight portion, Herba Verbenae 23 weight portion, Herba Pteridis Multifidae 32 weight portion, Herba panici repentics 28 weight portion, Herba Violae 22 weight portion, Indigo Naturalis 33 weight portion, Rhizoma Imperatae 30 weight portion, ten calyx eggplant 26 weight portions and Balaenoptera borealis Lesson 34 weight portion.

Described pharmaceutical composition also comprises Radix Puerariae, Herb Polygoni Chinensis, Herba Dianthi and Fructus Bruceae.

In described pharmaceutical composition, the parts by weight of each raw medicinal material are: see that blood lives 10-35 weight portion, halberd Ye Wawei 12-22 weight portion, Cirsium souliei (Franch.) Mattf. 10-30 weight portion, alkali ground Chinese small iris 11-31 weight portion, Radix Codonopsis 14-28 weight portion, Radix Chloranthi Serrati 15-35 weight portion, Bulbus Allii 15-25 weight portion, Herba cuscutae 13-33 weight portion, Semen Hoveniae (Fructus Hoveniae) 11-21 weight portion, Radix Et Rhizoma Rhei 12-26 weight portion, brocade line boomerang 14-36 weight portion, Radix Pulsatillae 11-33 weight portion, Herba Portulacae 10-30 weight portion, Radix Sanguisorbae 13-33 weight portion, Rhizoma Cimicifugae 10-20 weight portion, Radix Tinosporae 13-27 weight portion, Semen Euryales 14-34 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 11-32 weight portion, Folium Isatidis 10-30 weight portion, Flos Sophorae 12-36 weight portion, Herba Potentillae Chinensis 10-28 weight portion, Mel tank 14-36 weight portion, powder Rhizoma Smilacis Chinensis 10-20 weight portion, Climbing Viloet Herb 12-24 weight portion, Cortex Moutan 10-20 weight portion, Herba Verbenae 11-23 weight portion, Herba Pteridis Multifidae 12-32 weight portion, Herba panici repentics 15-35 weight portion, Herba Violae 12-24 weight portion, Indigo Naturalis 13-33 weight portion, Rhizoma Imperatae 12-31 weight portion, ten calyx eggplant 11-29 weight portions, Balaenoptera borealis Lesson 14-35 weight portion, Radix Puerariae 13-23 weight portion, Herb Polygoni Chinensis 14-36 weight portion, Herba Dianthi 12-32 weight portion and Fructus Bruceae 3-5 weight portion.

In described pharmaceutical composition, the parts by weight of each raw medicinal material are: see that blood lives 33 weight portions, halberd Ye Wawei 20 weight portion, Cirsium souliei (Franch.) Mattf. 25 weight portion, alkali ground Chinese small iris 31 weight portion, Radix Codonopsis 18 weight portion, Radix Chloranthi Serrati 32 weight portion, Bulbus Allii 25 weight portion, Herba cuscutae 31 weight portion, Semen Hoveniae (Fructus Hoveniae) 21 weight portion, Radix Et Rhizoma Rhei 24 weight portion, brocade line boomerang 32 weight portion, the Radix Pulsatillae 31 weight portion, Herba Portulacae 28 weight portion, Radix Sanguisorbae 32 weight portion, Rhizoma Cimicifugae 16 weight portion, Radix Tinosporae 25 weight portion, Semen Euryales 32 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 30 weight portion, Folium Isatidis 30 weight portion, Flos Sophorae 34 weight portion, Herba Potentillae Chinensis 22 weight portion, Mel tank 32 weight portion, powder Rhizoma Smilacis Chinensis 20 weight portion, Climbing Viloet Herb 14 weight portion, Cortex Moutan 16 weight portion, Herba Verbenae 23 weight portion, Herba Pteridis Multifidae 32 weight portion, Herba panici repentics 28 weight portion, Herba Violae 22 weight portion, Indigo Naturalis 33 weight portion, Rhizoma Imperatae 30 weight portion, ten calyx eggplant 26 weight portions and Balaenoptera borealis Lesson 34 weight portion, Radix Puerariae 21 weight portion, Herb Polygoni Chinensis 34 weight portion, Herba Dianthi 30 weight portion and Fructus Bruceae 3 weight portion.

The dosage form of described pharmaceutical composition is injectable powder, and its preparation method comprises the following steps:

The first step, described raw medicinal material is mixed in proportion, put into super micron mill and be ground into the mixed powder that the micron particles of 0.1 ~ 10 μm of volume average particle size and particle diameter be less than the nano-scale particle composition of 0.1 μm of volume average particle size, described mixed powder powder rate be at least 95%;

Second step, adding relative to the determining alcohol of the quality 3 ~ 5 times of described mixed powder in the mixed powder that the first step obtains is the ethanol of 85% ~ 95%, stirring and dissolving obtains alcoholic solution, alcoholic solution is left standstill 24 ~ 36 hours under the condition of 5 DEG C ~ 10 DEG C, adopt percolation with the speed of 1 ~ 2ml per minute slowly percolation, collect percolate, concentrated and dry, and put into super micron mill pulverizing 40 ~ 60 minutes at this, the micron particles of acquisition 0.1 ~ 10 μm of volume average particle size and particle diameter are less than the mixed powder of the nano-scale particle composition of 0.1 μm of volume average particle size, the powder rate that obtains of described mixed powder is at least 95%,

3rd step, gets mixed powder 10g ~ 20g that second step obtains, adds propylene glycol 30g ~ 50g, add 200ml water for injection, stirs and makes it dissolve, inject water to 1000ml further, then add 1g ~ 1.5g needle-use activated carbon, fully stir 30 ~ 50 minutes; Decarbonization filtering; With 0.22 ~ 0.24 μm of filtering with microporous membrane; Lyophilization obtains sterilized powder, subpackage embedding.

The dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:

The first step, Herba Portulacae, Radix Sanguisorbae, Rhizoma Cimicifugae, Radix Tinosporae, Semen Euryales, Hemsleya sphaerocarpa Kuang et A. M. Lu, Folium Isatidis, Flos Sophorae, Herba Potentillae Chinensis, Mel tank and powder Rhizoma Smilacis Chinensis are mixed in described ratio, with being the dissolve with ethanol of 85% ~ 95% relative to the determining alcohol of 3 ~ 5 times of mixture quality obtained, reflux was extracted after 2 ~ 4 hours, filter, obtain the first filtrate; By filtering residue again with the dissolve with ethanol of the determining alcohol 85% ~ 95% of 1 ~ 2 times relative to the mixture quality obtained, reflux was extracted after 1 ~ 2 hour, filtered, obtained the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.7 ~ 0.9g crude drug/mL, through the macroporous adsorptive resins eluting that volume is 5L, first use deionized water or the distilled water eluting of 5 times of resin column volumes, be 90% ~ 95% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collect ethanol elution, concentrating under reduced pressure removing ethanol, drying is also ground into powder;

Second step, Herba Verbenae, Herba Pteridis Multifidae, Herba panici repentics, Herba Violae, Indigo Naturalis, Rhizoma Imperatae, ten calyx eggplants and Balaenoptera borealis Lesson are mixed in described ratio, add the ethanol of the determining alcohol 85% ~ 95% of 2 ~ 4 times relative to the mixture quality obtained, heating and refluxing extraction 1 ~ 2 time, extracting solution merges, with the centrifugal remove impurity of tube centrifuge, when the extracting solution after remove impurity being evaporated under the condition of 60 DEG C ~ 70 DEG C 60 DEG C, relative density is the mastic of 1.20 ~ 1.23, and the mastic spray dryer after concentrated is dried to powder;

3rd step, mixes surplus stock medical material in described ratio, adds the water dissolution of 2 ~ 4 times relative to the mixture quality obtained, and heating decoction 2 ~ 4 hours, filters; By filtering residue again with the water dissolution of 1 ~ 2 times relative to the mixture quality obtained, again heat decoction 1 ~ 2 hour, filter; Merge the filtrate of twice, concentrating under reduced pressure removing moisture, drying is also ground into powder;

4th step, the powder mixing first step, second step and the 3rd step obtained, is screened into 80 ~ 120 object fine powders subsequently, is dropped in mixing and blending machine by the fine powder of acquisition and stirs, namely obtain powder.

Herba Potentillae Chinensis the invention has the beneficial effects as follows: pharmaceutical composition of the present invention has effect of heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, cure mainly the piglet diarrhea at 2-3 monthly age, the immunity of pig can also be improved simultaneously, strengthen its resistance against diseases, in treatment swine dysentery, there is curative effect fast, treatment short treating period, the advantage such as have no side effect.

Detailed description of the invention

Pharmaceutical composition of the present invention follows heat-clearing and toxic substances removing, the principle of eliminating pathogenic heat from blood to cure dysentery, provide the pharmaceutical composition that a kind treats piglet diarrhea, comprise following raw medicinal material: see that blood is lived, halberd Ye Wawei, Cirsium souliei (Franch.) Mattf., alkali ground Chinese small iris, Radix Codonopsis, Radix Chloranthi Serrati, Bulbus Allii, Herba cuscutae, Semen Hoveniae (Fructus Hoveniae), Radix Et Rhizoma Rhei, brocade line boomerang, the Radix Pulsatillae, Herba Portulacae, Radix Sanguisorbae, Rhizoma Cimicifugae, Radix Tinosporae, Semen Euryales, Hemsleya sphaerocarpa Kuang et A. M. Lu, Folium Isatidis, Flos Sophorae, Herba Potentillae Chinensis, Mel tank, powder Rhizoma Smilacis Chinensis, Climbing Viloet Herb, Cortex Moutan, Herba Verbenae, Herba Pteridis Multifidae, Herba panici repentics, Herba Violae, Indigo Naturalis, Rhizoma Imperatae, ten calyx eggplants, Balaenoptera borealis Lesson, Radix Puerariae, Herb Polygoni Chinensis, Herba Dianthi and Fructus Bruceae.

The pharmacological property of above-mentioned each raw medicinal material is as follows:

Alkali ground Chinese small iris: nature and flavor: sweet, light, cool.Cure mainly: heat-clearing and toxic substances removing, diuresis, hemostasis.

Cirsium souliei (Franch.) Mattf.: nature and flavor: sweet is bitter.Cure mainly: removing heat from blood, scattered inflammatory swelling.Cure mainly haematemesis, epistaxis, hematuria, metrorrhagia, jaundice, carbuncle sore.

Ten calyx eggplants: nature and flavor: puckery are cool.Cure mainly: expelling phlegm for arresting cough, heat-clearing and toxic substances removing.Leaf: cough and asthma; Herb: rabies, furuncle redness is controlled in external, traumatic hemorrhage.

Balaenoptera borealis Lesson: nature and flavor: bitter, puckery, cold.Cure mainly: heat-clearing and toxic substances removing, cooling blood for hemostasis.For haematemesis of coughing, metrorrhagia, swelling and pain of hemorrhoid, furuncle of finger.

Herba panici repentics: nature and flavor: sweet is flat.Cure mainly: heat clearing away suppressing the hyperactive liver, eliminating damp, detoxifying.Cure mainly hypertension, sinusitis, epistaxis, damp-heat vaginal discharge, urinary tract infection, intercostal neuralgia, icterohepatitis, a fishbone or other bone caught in the throat larynx.

Powder Rhizoma Smilacis Chinensis: nature and flavor: sweet, flat.Cure mainly: heat clearing away, except pathogenic wind toxic.

Climbing Viloet Herb: nature and flavor: bitter, micro-pungent, cold.Cure mainly: heat-clearing and toxic substances removing, detumescence and apocenosis.For hepatitis, pertussis, conjunctival congestion and swelling pain; Acute mastitis is controlled in external, furuncle, carbuncle furuncle, herpes zoster, venom, traumatic injury.

Mel tank: nature and flavor: micro-hardship, cold.Cure mainly: heat-clearing and toxic substances removing, expelling wind and removing dampness.For osteomyelitis, periostitis, icterohepatitis, traumatic injury, bones and muscles pain due to rheumatism; Furuncle is controlled in external.

See that blood is lived: nature and flavor: micro-acid, puckery, cool.Cure mainly: blood pressure lowering, hemostasis, antidotes against snake bite.For hypertension, hemoptysis of pulmonary tuberculosis; Venom is controlled in external, traumatic hemorrhage.

Semen Hoveniae (Fructus Hoveniae): nature and flavor: son: sweet is flat.Cure mainly: clearing away heat and promoting diuresis, cough-relieving relieving restlessness, alcoholic intoxication.For calentura excessive thirst, singultus, vomiting, dysuria, alcoholism.

Brocade line boomerang: source: Rosaceae brocade line boomerang Pptentilla leuconata D. Don, with all herbal medicine.Nature and flavor: pungent, bitter are flat.Cure mainly: heat-clearing and toxic substances removing.Cure mainly acute appendicitis, wind heat celostomia, dysentery.

Radix Chloranthi Serrati: source: " national Chinese herbal medicine compilation ".Source: be golden Li Lanke gold chestnut epidendrum Radix Chloranthi Serrati Chloranthus serratus (Thunb.) Roem. et Schult., with root or all herbal medicine.Summer, autumn excavate herb, clean, dry; Or root is cut down, dry respectively.Nature and flavor: pungent, warm.Poisonous.Cure mainly: relaxing muscles and tendons and activating QI and blood in the collateral, wind-expelling pain-stopping, subduing swelling and detoxicating.For traumatic injury, lumbago, skelalgia of rheumatism, furuncle swelling toxin, venom.

Herba Violae: bitter, acrid, cold.GUIXIN, Liver Channel, heat-clearing and toxic substances removing, removing heat from blood are subsided a swelling.

Herba Portulacae: cold in nature, sweet in the mouth acid; Enter the heart, liver, spleen, large intestine channel, heat-clearing and toxic substances removing, diuretic dries, loose blood detumescence, dust-collection sterilization, anti-inflammatory analgetic.

Halberd Ye Wawei: nature and flavor: bitter, sweet, flat.Cure mainly: relieving stranguria by diuresis, eliminating heat from the lung.Cure mainly oedema due to nephritis, urinary tract infection, calculus of urethra, cough due to lung-heat, spitting of blood, bronchial asthma, the diseases such as pharyngolaryngitis.

The Radix Pulsatillae: bitter in the mouth, cold in nature; Return stomach warp, large intestine channel, heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, removing dampness and killing parasites.

Radix Sanguisorbae: picric acid, cold, nontoxic; Return liver, lung, kidney, large intestine channel, cooling blood for hemostasis, heat-clearing and toxic substances removing, detumescence sore.Deficient cold type is had blood in stool, metrorrhagia and the hemorrhage silt person of having are cautious use of;

Folium Isatidis: bitter, cold; Enter liver, the heart, stomach warp, heat-clearing and toxic substances removing, cooling blood for hemostasis.Deficiency and coldness of spleen and stomach person avoids use;

Indigo Naturalis: salty in the mouth, cold in nature, enter Liver Channel, heat-clearing and toxic substances removing, cooling blood for hemostasis.

Flos Sophorae: bitter in the mouth, cold nature; Return liver, lung, the heart, large intestine channel, cooling blood for hemostasis, purging liver-fire.Deficiency and coldness of spleen and stomach fever due to yin deficiency and being cautious use of without excess-fire person;

Radix Codonopsis: property is put down, sweet in the mouth, micro-acid; Return spleen, lung meridian.Invigorating the spleen and replenishing QI, spleen invigorating lung benefiting.For deficiency of the spleen and lung, cardiopalmus of breathing hard, anorexia and loose stool, dyspnea due to deficiency is coughed, and interior-heat is quenched one's thirst.These product are clinical conventional Qi-tonifying drug, function invigorating the spleen to benefit the lung, and effect person of modern times joins and is more weak, is applicable to various deficiency of vital energy deficiency person.Contained saponin, inulin, Alkaloid, starch etc. have strong effect in various degree to human body multi viscera, can improve the adaptability of human body.Have antiulcer, can increase intestinal tension force, regulate stomach motion, in the intestines and stomach coordinating preparation, reuse the intestines and stomach regulation of mental activities agent of Radix Codonopsis to chronic diarrhea, ulcerative colitis, antral gastritis, chronic atrophic gastritis etc. quite have effect.

Bulbus Allii: warm in nature, acrid in the mouth is put down; Enter spleen, stomach, lung meridian, supplement to the Herbal: " the just unfavorable order of food, polyphagia is bright.Food of a specified duration makes us serum, makes hair white ".Have that warming middle-JIAO helps digestion, the stagnant gas of row, warming the spleen and stomach, removing food stagnancy, removing toxic substances, parasite killing effect, cure mainly eating accumulation, coldness and pain in the epigastrium, edema distension, have loose bowels, dysentery, malaria, pertussis, swollen ulcer drug, white bald tinea skin ulcer, snake bite and insect sting and the card such as ancylostome, pinworm.Though Bulbus Allii has the merit of parasite killing removing toxic substances cold relieving stomach invigorating, but character belongs to damp and hot product, simultaneously also irritant, liver, lung, stomach and eyes can be stimulated, if suffer from liver-heat or hepatitis, taste fire severe one, eyes pain or have inflammation person that it should be guarded against.Bulbus Allii polyphagia then the impairment of the spleen, damage lung, bad liver, hinder ear, raw expectorant, send out and cough.These potential effects that Bulbus Allii has, for prevention with improve atherosclerotic arteriosclerosis, prevent and treat heart disease, open a brand-new natural nursing approach.Meanwhile, Bulbus Allii also has certain effect to reduction hypertension.As long as keep blood normal, be just not easy to suffer from the diseases such as hypertension, heart disease, cerebral hemorrhage.

Herba cuscutae: nature and flavor: sweetness and bitterness; Flat.Gui Jing: liver; Kidney; Urinary bladder channel.Cure mainly: heat-clearing and toxic substances removing; Cooling blood for hemostasis; Invigorating spleen to remove dampness.Main haematemesis; Epistaxis; Have blood in stool; Metrorrhagia; Stranguria with turbid discharge; Leukorrhagia; Dysentery; Yellow cellulitis; Loose stool; Conjunctival congestion and swelling pain; Laryngopharynx swelling and pain; Swollen ulcer drug; Miliaria.

Herba cuscutae Herba cuscutae Herba cuscutae Herba cuscutae Herba cuscutae Herba cuscutae Radix Et Rhizoma Rhei: bitter, cold; Return spleen, stomach, large intestine, Liver Channel.Purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body, eliminating blood stasis and inducing menstruation.For excess-heat constipation, stagnant stomachache, dysentery is not well, jaundice due to damp-heat, and heat in blood tells nosebleed, conjunctival congestion, pharyngeal swelling, abdominalgia with intestinal abscess, carbuncle furuncle, blood stasis amenorrhea, traumatic injury, external treatment burn due to hot liquid or fire; Upper gastrointestinal hemorrhage.Ulcer and gastritis merge hemorrhage, and particularly based on melena, amount of bleeding is person within 500 milliliters; Accompany taste-blindness rate merging hemorrhage to apoplexy and the patient of blood-clotting agent should not be used, especially to accompanying yellowish fur, constipation and having the patient absorbing heat more suitable; Invalid with other haemostatic medicaments, and unsuitable operative treatment person.

Rhizoma Cimicifugae: acrid in the mouth, sweet, cold nature; Return lung meridian, spleen channel, large intestine channel, stomach warp." property of medicine opinion ": " control little eclampsia, infantile convulsion, the hot disease of seasonal epidemic pathogens.Can control the swollen pain of ability to speak wind NI, root of the tooth floats rotten stench, pyretic toxicity pus and blood.Except cardiopulmonary pathogenic wind toxic heat is obstructed and constipated, aphtha, unhappy.Treat carbuncle, smallpox; Decocting silk floss is stained with and is wiped in skin ulcer." Compendium of Material Medica: " speckle removing rash, clots absorbing blood are controlled sun and are fallen into dizzy fortune, the empty pain of the breast side of body, heavy after chronic diarrhea dysentery, lose turbid, leukorrhagia, metrorrhagia, stranguria with blood, hematochezia, impotence cold foot." function heat-clearing and toxic substances removing, rash.Not smooth, maculopapulae due to toxic heat, diseases caused by exogenous pathogenic factor exterior syndrome is thoroughly sent out, weight, women's leukorrhagia, metrorrhagia under epidemic disease fire-toxin, aphtha, pharyngalgia, headache cold and heat, carbuncle sore tumefacting virus, sinking of QI of middle-JIAO, diarrhea due to hypofunction of the spleen, chronic dysentery for measles.

Radix Tinosporae: bitter, cold.Return lung, large intestine channel." property of medicine is examined ": " removing toxic substances.Throat numbness is anxious, and erosion of mucous membrane of the oral cavity should take.The red furuncle Skin-disease of cellulitis carbuncle carbuncle on the back ， inflammation, snakes and scorpions insect bite, mill is coated with.Control ophthalmalgia, bulge of the ear, cough due to heat, haze miasma, tells nosebleed, all outer diseases." " the little knowledge in Citrus chachiensis Hort. garden ": " and inside and outside stagnation of pathogenic heat of dispelling, all over vicious, disappear communicable subtropical diseases, two single throat moth and toothache, and shave contains; Mill is coated with furuncle swelling toxin." function heat-clearing and toxic substances removing, sore-throat relieving, reducing swelling and alleviating pain.For laryngopharynx swelling and pain, carbuncle furunculosis, has loose bowels, dysentery, gastral cavity abdomen burning pain.Except decocting soup and taking orally, also can mash external application with fresh grass.For traumatic hemorrhage, available Radix Glycyrrhizae levigation, spreading is wrapped up; Swell and ache with controlling blood stasis, appropriate with fresh grass, mash external application.

Herba Dianthi: nature and flavor: bitter, cold.Gui Jing: GUIXIN, small intestine meridian.Cure mainly: inducing diuresis for treating stranguria syndrome, removing blood stasis stimulates the menstrual flow.For pyretic stranguria, stranguria with blood, stranguria caused by urinary stone, drenches the puckery pain of drop, menstruation apolipsis.

Fructus Bruceae: source: this product is the dry mature fruit of quassia Fructus Bruceae Brucea javanica (L.) Merr..Gather during fruit maturation in autumn, removing impurity, dries.Nature and flavor: bitter, cold; Slightly poisonous.Gui Jing: return large intestine, Liver Channel.Cure mainly: heat-clearing and toxic substances removing, preventing the attack (or recurrence) of malaria, dysentery relieving, corrosion wart.For dysentery, malaria; External treatment wart, clavus.

Semen Euryales: sweet in the mouth, puckery, property is put down.Return spleen, kidney channel." herbal classic ": " main arthralgia chiefly caused by damp pathogen spinal column gonalgia, invigorating middle warmer is except sudden and violent disease, and beneficial vital essence, Qiang Zhi, makes normal hearing and eyesight." there is supplementing the kidney to control the nocturnal, spleen reinforcing antidiarrheal, the function of damp eliminating leukorrhagia stopping, cures mainly emission, diarrhea due to hypofunction of the spleen, insufficiency of the spleen nebulousurine, is with inferior card.Before and after all diseases caused by exogenous pathogenic factor, malaria dysentery infantile malnutrition hemorrhoid, stagnation of QI painful abdominal mass is swollen, dark coloured urine constipation, eats and not transporting and new puerperal all avoids it.Record according to Compendium of Material Medica, Semen Euryales cure mainly arthralgia chiefly caused by damp pathogen, spinal column gonalgia, invigorating middle warmer, except sudden and violent disease, the strong will of beneficial vital essence, make normal hearing and eyesight, appetizing helps gas, quenches the thirst.

Hemsleya sphaerocarpa Kuang et A. M. Lu: puckery micro-hardship, flat, nontoxic." the practical Chinese herbal medicine in Guangxi newly selects ": " removing toxic substances, convergence, granulation promoting.Control venom, burn, dysentery, diarrhoea." " register planted by Guangxi medicine ": " and heat clearing away. convergence, go to amass, puerperal enriches blood.Control the intestines and stomach damp and hot, bloody dysentery, erosion of the oral mucosa, carbuncle, hemorrhoid, burn." Rhizoma Polygoni coriacei mainly contains heat-clearing and toxic substances removing, movable blood soft hard, removes long-pending dysentery relieving, convergence granulation promoting, antidote against snake bite; Blue or green Hemsleya sphaerocarpa Kuang et A. M. Lu primary efficacy has expelling wind and removing dampness, dissipating blood stasis analgesic therapy, among the people long-term as removing toxic substances and in, expelling wind and removing dampness, hard masses softening and resolving medicinal application, as hemorrhoidal hamorrhage vomiting and diarrhoea, rheumatalgia, worm Serpentis wound, rock swell addiction knot etc.More modern pharmacological researches show infection that three kinds of medicines all have, antiinflammatory, the effect such as anticancer.

Herba Potentillae Chinensis: nature and flavor: bitter, cold.Gui Jing: return liver, large intestine channel.Cure mainly: heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery.For bloody dysentery stomachache, chronic dysentery is more than, bleeding hemorrhoids, carbuncle sore tumefacting virus.

Herba Potentillae Chinensis Cortex Moutan: bitter in the mouth, pungent, cold nature, enters the heart, liver, kidney channel." herbal classic ": " main cold and heat, apoplexy Chi Zong, convulsion, infantile convulsion pathogen, except the hard blood stasis of disease stays house the intestines and stomach, settling five organs, treat carbuncle skin ulcer." " Japan hanako materia medica ": " except pathogen, happy color, clearance natural fibre line of meat blood vessels, evacuation of pus, logical menstruation, disappear and flutter damage blood stasis, reuniting the fractured tendons and bones, except migratory arthralgia, fall tire lower eyelid, puerperal all cold and hot vim and vigour."。Compendium of Material Medica: " nourishing YIN to lower pathogenic fire separates speckle poison, throat, diuresis stasis.Descendant specially controls fire mutually with yellow tiller, does not know that the merit of Cortex Moutan more wins also.Red colored person's profit, the person of spending in vain mends, and people also seldom realizes, and should distinguish it." there is the effect of clearing away heat and cooling blood, promoting blood circulation to remove blood stasis, be mainly used in the treatment epidemic febrile disease pathogenic heat attacking blood system in febrile diseases, send out speckle, tell nosebleed, calentura later stage hot Fu Yin divides heating, deficiency of YIN osteopyrexia and fever, amenorrhea due to stagnation of blood, dysmenorrhea, carbuncle sore tumefacting virus, falls and flutter the card such as the pain of injury, beriberoid pyretic arthralgia.Be apt to purify the blood, and invigorate blood circulation, thus have effect of cool the blood dissipate blood stasis, make blood flow freely and not stay the stasis of blood, the absurd clearly and not row of heat in blood.Therefore to diseases such as intense heat in the blood, Liver and kidney hyperactivity of fire and stagnation of blood stasis, all rely on as key medicine.Cortex Moutan can increase coronary flow to the anesthetized dog heart, reduces cardiac output, reduces the effect of left room work done.The mice heating that paeonol oral disposition typhoid fever, paratyphoid vaccine cause has refrigeration function, and reduces normal mouse body temperature.

Herba Verbenae: bitter, cool.Return liver, spleen channel." Bencao Jingshu ": " Herba Verbenae is originally the medicine of removing heat from blood removing blood stasis.Bottom abscess person, the pole of heat in blood, furthermore damp and hot, therefore blood stains are turbid forms skin ulcer, and have worm also.The cool pyrolysis of blood, the broken and row of dirty person, waste and be not recovered.CHEN Zang-Qi calls its removing blood stasis parasite killing, also this meaning ear." " Japan hanako materia medica ": " logical menstruation, controls married woman's vim and vigour tripe swollen, and the moon waits irregular." venbenalin is in a small amount excited to SNE, suppress in a large number; Galactopoiesis can be promoted to mammal.Promoting blood circulation to remove blood stasis, preventing the attack (or recurrence) of malaria, removing toxic substances, inducing diuresis to remove edema.For lump in the abdomen, amenorrhea dysmenorrhea, malaria, sore throat, carbuncle, edema, pyretic stranguria.Herba Verbenae tea have refresh oneself, mild emotion, eliminate exert one's utmost effort, effect of facilitating digestion, it effectively can also solve the puzzlement of lower part of the body edema in addition, is particularly suitable for doing work room because being sitting in for a long time and causing that the swollen white collar of lower limb is drunk.

Radix Puerariae: nature and flavor: sweet, pungent, cool.Gui Jing: return spleen, stomach warp.Cure mainly: expelling pathogenic factors from muscles for reducing heat, promote the production of body fluid, rash, yang invigorating antidiarrheal.For fever caused by exogenous pathogens headache, stiff nape and back, thirsty, quench one's thirst, measles without adequate eruption, hematodiarrhoea, has loose bowels; Hypertension neck pain.

Herb Polygoni Chinensis: source: " Bencao Tujing ".Nature and flavor: acid is sweet, cool.Cure mainly: clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body.Control and have loose bowels, dysentery, jaundice, wind heat pharynx pain, weak dizzy, summer non-acclimation in children, shies and jerks, women leucorrhea, carbuncle eczema, traumatic injury.

Herba Pteridis Multifidae: light, micro-hardship is cool." the SHENGCAO property of medicine is standby to be wanted ": " wash infantile malnutrition, furuncle, hemorrhoid, loose poison, applies skin ulcer.Control Serpentis and sting all poison, knife injury, energy hemostasia and promoting granulation, spring juice rectification clothes, slag applies affected part.Grinding into powder harvests and store controls pain due to disorder of QI." " conventional Chinese herbal medicine handbook ": " heat clearing away, dampness removing, removing heat from blood.Control bacillary dysentery, enteritis, fever caused by exogenous pathogens, urinary tract infection, leucorrhea, parotitis, furuncle, eczema." clearing away heat-damp and promoting diuresis, removing toxic substances dysentery relieving, cooling blood for hemostasis.For dysentery, gastroenteritis, hepatitis, urinary system infection, cat fever, laryngopharynx swelling and pain, leucorrhea, metrorrhagia, pesticide intoxication; Traumatic hemorrhage is controlled in external, burn and scald.

Rhizoma Imperatae: sweet in the mouth, cold in nature, enter lung, stomach, urinary bladder channel." Bencao Tujing ": " Rhizoma Imperatae, the present has it everywhere.Spring sprouts, and cloth ground is as pin, and the thatch pin called between custom, also can eat, very beneficial children's.Xia Sheng spends in vain, so fine, withered to the autumn, and its root, to pure white, also very sweet and refreshing, adopted root June and used." Compendium of Material Medica: " emesis is defeated in battle all blood, typhoid fever hiccup, lung-heat dyspnea with rapid respiration, edema, jaundice, alcoholic intoxication." have the effect of cooling blood for hemostasis, clearing away heat and promoting diuresis, be mainly used in haematemesis, hemoptysis that lung stomach has accumulated heat, damp invasion of lower energizer pents up the hematuria of bladder, calentura excessive thirst taking fluids profusely, gastropyretic vomiting, the cards such as jaundice due to damp-heat.Rhizoma Imperatae decoct and water logging agent gavage, and have diuresis to normal rabbits, administration 5-10 days, and diuresis is the most obvious.

Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.

Embodiment 1 pharmaceutical composition 1

Embodiments provide a kind of pharmaceutical composition for the treatment of piglet diarrhea, be made up of the raw medicinal material of following parts by weight: see that blood lives 33 weight portions, halberd Ye Wawei 20 weight portion, Cirsium souliei (Franch.) Mattf. 25 weight portion, alkali ground Chinese small iris 31 weight portion, Radix Codonopsis 18 weight portion, Radix Chloranthi Serrati 32 weight portion, Bulbus Allii 25 weight portion, Herba cuscutae 31 weight portion, Semen Hoveniae (Fructus Hoveniae) 21 weight portion, Radix Et Rhizoma Rhei 24 weight portion, brocade line boomerang 32 weight portion, the Radix Pulsatillae 31 weight portion, Herba Portulacae 28 weight portion, Radix Sanguisorbae 32 weight portion, Rhizoma Cimicifugae 16 weight portion, Radix Tinosporae 25 weight portion, Semen Euryales 32 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 30 weight portion, Folium Isatidis 30 weight portion, Flos Sophorae 34 weight portion, Herba Potentillae Chinensis 22 weight portion, Mel tank 32 weight portion, powder Rhizoma Smilacis Chinensis 20 weight portion, Climbing Viloet Herb 14 weight portion, Cortex Moutan 16 weight portion, Herba Verbenae 23 weight portion, Herba Pteridis Multifidae 32 weight portion, Herba panici repentics 28 weight portion, Herba Violae 22 weight portion, Indigo Naturalis 33 weight portion, Rhizoma Imperatae 30 weight portion, ten calyx eggplant 26 weight portions and Balaenoptera borealis Lesson 34 weight portion.

Its preparation method comprises the following steps:

The first step, described raw medicinal material is mixed in proportion, put into super micron mill and be ground into the mixed powder that the micron particles of 3 μm of volume average particle size and particle diameter be less than the nano-scale particle composition of 0.1 μm of volume average particle size, described mixed powder powder rate be at least 95%;

Second step, adding relative to the determining alcohol of the quality 4 times of described mixed powder in the mixed powder that the first step obtains is the ethanol of 90%, stirring and dissolving obtains alcoholic solution, alcoholic solution is left standstill 30 hours under the condition of 9 DEG C, adopt percolation with the speed of 2ml per minute slowly percolation, collect percolate, concentrated and dry, and put into super micron mill pulverizing 50 minutes at this, the micron particles of acquisition 4 μm of volume average particle size and particle diameter are less than the mixed powder of the nano-scale particle composition of 0.1 μm of volume average particle size, the powder rate that obtains of described mixed powder is at least 95%,

3rd step, gets the mixed powder 15g that second step obtains, adds propylene glycol 40g, add 200ml water for injection, stirs and makes it dissolve, inject water to 1000ml further, then add 1.5g needle-use activated carbon, fully stirs 40 minutes; Decarbonization filtering; With 0.23 μm of filtering with microporous membrane; Lyophilization obtains sterilized powder, subpackage embedding.

Embodiment 2 pharmaceutical composition 2

Embodiments provide a kind of pharmaceutical composition for the treatment of piglet diarrhea, be made up of the raw medicinal material of following parts by weight: see that blood lives 33 weight portions, halberd Ye Wawei 20 weight portion, Cirsium souliei (Franch.) Mattf. 25 weight portion, alkali ground Chinese small iris 31 weight portion, Radix Codonopsis 18 weight portion, Radix Chloranthi Serrati 32 weight portion, Bulbus Allii 25 weight portion, Herba cuscutae 31 weight portion, Semen Hoveniae (Fructus Hoveniae) 21 weight portion, Radix Et Rhizoma Rhei 24 weight portion, brocade line boomerang 32 weight portion, the Radix Pulsatillae 31 weight portion, Herba Portulacae 28 weight portion, Radix Sanguisorbae 32 weight portion, Rhizoma Cimicifugae 16 weight portion, Radix Tinosporae 25 weight portion, Semen Euryales 32 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 30 weight portion, Folium Isatidis 30 weight portion, Flos Sophorae 34 weight portion, Herba Potentillae Chinensis 22 weight portion, Mel tank 32 weight portion, powder Rhizoma Smilacis Chinensis 20 weight portion, Climbing Viloet Herb 14 weight portion, Cortex Moutan 16 weight portion, Herba Verbenae 23 weight portion, Herba Pteridis Multifidae 32 weight portion, Herba panici repentics 28 weight portion, Herba Violae 22 weight portion, Indigo Naturalis 33 weight portion, Rhizoma Imperatae 30 weight portion, ten calyx eggplant 26 weight portions and Balaenoptera borealis Lesson 34 weight portion, Radix Puerariae 21 weight portion, Herb Polygoni Chinensis 34 weight portion, Herba Dianthi 30 weight portion and Fructus Bruceae 3 weight portion.

Its preparation method is as follows:

The first step, Herba Portulacae, Radix Sanguisorbae, Rhizoma Cimicifugae, Radix Tinosporae, Semen Euryales, Hemsleya sphaerocarpa Kuang et A. M. Lu, Folium Isatidis, Flos Sophorae, Herba Potentillae Chinensis, Mel tank and powder Rhizoma Smilacis Chinensis are mixed in described ratio, with being the dissolve with ethanol of 90% relative to the determining alcohol of 4 times of mixture quality obtained, reflux was extracted after 3 hours, filter, obtain the first filtrate; By filtering residue again with the dissolve with ethanol of the determining alcohol 90% of 2 times relative to the mixture quality obtained, reflux was extracted after 2 hours, filtered, obtained the second filtrate; Merge the first filtrate and the second filtrate, decompression recycling ethanol to be concentrated into liquor strength be 0.8g crude drug/mL, through the macroporous adsorptive resins eluting that volume is 5L, first use deionized water or the distilled water eluting of 5 times of resin column volumes, be 90% ethanol elution with the determining alcohol of 3 times of resin column volumes again, collect ethanol elution, concentrating under reduced pressure removing ethanol, drying is also ground into powder;

Second step, Herba Verbenae, Herba Pteridis Multifidae, Herba panici repentics, Herba Violae, Indigo Naturalis, Rhizoma Imperatae, ten calyx eggplants and Balaenoptera borealis Lesson are mixed in described ratio, add the ethanol of the determining alcohol 90% of 3 times relative to the mixture quality obtained, heating and refluxing extraction 2 times, extracting solution merges, with the centrifugal remove impurity of tube centrifuge, when the extracting solution after remove impurity being evaporated under the condition of 65 DEG C 60 DEG C, relative density is the mastic of 1.22, and the mastic spray dryer after concentrated is dried to powder;

3rd step, mixes surplus stock medical material in described ratio, adds the water dissolution of 3 times relative to the mixture quality obtained, and heating decoction 3 hours, filters; By filtering residue again with the water dissolution of 1.5 times relative to the mixture quality obtained, again heat decoction 1.5 hours, filter; Merge the filtrate of twice, concentrating under reduced pressure removing moisture, drying is also ground into powder;

4th step, the powder mixing first step, second step and the 3rd step obtained, is screened into 100 object fine powders subsequently, is dropped in mixing and blending machine by the fine powder of acquisition and stirs, namely obtain powder.

The toxicity test of pharmaceutical composition:

1, trial drug

Choose and see that blood lives in 33g, halberd Ye Wawei 20g, Cirsium souliei (Franch.) Mattf. 25g, alkali ground Chinese small iris 31g, the heavy g of Radix Codonopsis 18, Radix Chloranthi Serrati 32g, Bulbus Allii 25g, Herba cuscutae 31g, Semen Hoveniae (Fructus Hoveniae) 21g, Radix Et Rhizoma Rhei 24g, brocade line boomerang 32g, Radix Pulsatillae 31g, Herba Portulacae 28g, Radix Sanguisorbae 32g, Rhizoma Cimicifugae 16g, Radix Tinosporae 25g, Semen Euryales 32g, Hemsleya sphaerocarpa Kuang et A. M. Lu 30g, Folium Isatidis 30g, Flos Sophorae 34 weight portion, Herba Potentillae Chinensis 22g, Mel tank 32g, powder Rhizoma Smilacis Chinensis 20g, Climbing Viloet Herb 14g, Cortex Moutan 16g, Herba Verbenae 23g, Herba Pteridis Multifidae 32g, Herba panici repentics 28g, Herba Violae 22g, Indigo Naturalis 33g, Rhizoma Imperatae 30g, ten calyx eggplant 26g and Balaenoptera borealis Lesson 34g mix, be ground into powder, added by acquisition powder in 1700g cold water and soak 25 hours, with slow fire boiling, decocting time is 1.5 hours, decoction liquor is filtered, obtains the first filtrate, collects filtering residue, again add in 1700g cold water by filtering the filtering residue obtained, with slow fire boiling 3 times, each decoction 1 hour, filters and merges filtrate, obtaining the second filtrate, merge twice filtrate, concentrating under reduced pressure remove portion water, obtain decoction, the crude drug concentration of decoction is 3g/ml.

2, test method: select SD rat, give the decoction of the Chinese medicine additive of the present invention of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, within after last administration 24 hours, respectively group is alive kills 1/2 animal (male and female half and half), and all the other 1/2 animals continue observations 2 weeks rear living and kill.Duration of test observes outward appearance, general behavior, food ration, the body weight change of animal, within after administration 90 days, within 2 weeks, carries out the index checking such as hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology with drug withdrawal.Result of the test shows: Chinese medicine additive of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception; Three dosage groups and matched group hematological examination, blood biochemical analysis, urine biochemical analysis all in normal range, without significant difference between group; Each group of main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.This test dosage is respectively 180,60,20 times of clinical medicine dose, rat is had no significant effect in high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) administration in continuous 90 days according to result of the test Chinese medicine preparation of the present invention, without clear and definite toxicity target organ and sensitive indicator, convalescent period observation also has no retardance toxic reaction, points out the dose safety of Chinese medicine preparation clinical practice of the present invention higher.

Contrast test

Liaocheng of Shandong Province pig plant, in by the end of December, 2012, find that becoming thin in various degree appears in 450 77 age in days piglets nearly, mucus or hemorrhagic dysentery, to have loose bowels, feces is medicated porridge sample or water sample, include mucus, mucosa or blood are feature, even there is dead piglet, the mucus got with the blood streak in fresh excreta is a little, or get fritter and have the direct smear of the colorectal mucosa of obvious pathological changes, fix through flame after natural drying in atmosphere, with ammonium oxalate crystal violet liquid, Ji's nurse dyeing liquor dyes 4 minutes, after smear washing is dried in the shade, examine under a microscope, can see that swine dysentery is crawled spirillum, or above-mentioned pathological material of disease 1 droplet is placed on microscope slide, dripping 1 normal saline, mixing, then cover slide, with dark-field microscopy (400 times), when finding that there is the thalline vivaciously moved in Serpentis sample, swine dysentery can be diagnosed as.

Choose the ill piglet 180 that 77 ages in days are made a definite diagnosis, 180 ill pigs are divided into three groups at random, be respectively embodiment group 1, embodiment group 2 and matched group, the ill pig of three groups all without significant difference, has comparability in body weight, the state of an illness etc.Embodiment group 1, embodiment group 2 and matched group use the powder of the injectable powder of the embodiment of the present invention 1, embodiment 2 and conventional medicament to treat respectively; Wherein, injectable powder adopts subcutaneous injection, each 5g, once a day, and injection 6 days continuously; The using method of powder be by powder according in the part by weight conventional feed of 5g:500g, feed continuously 6 days; Conventional medicament is subcutaneous injection hyper-immune serum 0.2 milliliter, conventional feed of feeding.Experimental period is 6 days, takes each group of ill pig body weight respectively before and after experiment, and period records recovery from illness situation and the death toll of ill pig, calculates average daily gain and cure rate, and the time of recovery from illness number compares, result as table 1, shown in table 2 and table 3.Wherein, cure: Normal-weight, no longer include mucus or hemorrhagic dysentery, medicated porridge sample or water sample is there is not in feces, include the phenomenon of mucus, mucosa or blood, the mucus that fresh excreta is got in sampling is a little, fixes in atmosphere after natural drying through flame, dye 4 minutes with ammonium oxalate crystal violet liquid, Ji's nurse dyeing liquor, smear washing is examined under a microscope after drying in the shade, and does not see that swine dysentery is crawled spirillum; Invalid: clinical symptoms is not improved and even increased the weight of or death.

Table 1 is group piglet average weight synopsis respectively

Group	Sum	Average weight before experiment	Average weight after test	Average daily gain
					Embodiment group 1	60	30.8kg	35.4kg	0.77kg/d
Embodiment group 2	60	30.7kg	35.7kg	0.83 kg/d
					Matched group	60	30.8kg	32.1kg	0.22kg/d

Data from table 1: compared with matched group, use the ill pig of Drug therapy of the present invention, the piglet that its average weight is treated than traditional therapy exceeds 3.3kg and 3.6kg respectively, and average daily gain exceeds 0.55kg/d and 0.61kg/d respectively.

The therapeutic effect synopsis of piglet respectively organized by table 2

Group	Sum	Recovery from illness number	Invalid number	Cure rate
					Embodiment group 1	60	60	0	100%
Embodiment group 2	60	60	0	100%
					Matched group	60	47	13	78.3%

Data from table 2: compared with conventional medicament, the medicine of the embodiment of the present invention 1 and 2 has advantage that is evident in efficacy, cheap, that have no side effect in the little swine fever for the treatment of.

Known by contrast test: compared with conventional medicament, the pharmaceutical composition of the embodiment of the present invention 1 and 2 has toxic and side effects advantage little, evident in efficacy in the little swine fever for the treatment of, but also can be good at the normal growth ensureing piglet.

The time comparative example (%) of curing number for 6 days afterwards treated respectively by table 3 respectively group

Group	Number of cases	1-2 days	3-4 days	5-6 days
					Embodiment group 1	60	（56）93.3%	（4）6.7%	（0）0%
Embodiment 2	60	（58）96.7%	（2）3.3%	（0）0%
					Matched group	47	（3）6.4%	（7）14.9%	（37）78.7%

Data from table 3: pharmaceutical composition of the present invention, to treatment piglet pujos blancos, relative to conventional medicament on therapeutic effect, has significant improvement, obviously shortens and treats the course for the treatment of.

Model case:

300 77 age in days piglets that in January, 2014, Taian Shandong self-employed pig raiser raised, nearly becoming thin appears in 100 2-3 monthly age piglets, mucus or hemorrhagic dysentery, to have loose bowels, feces is medicated porridge sample or water sample, include mucus, mucosa or blood phenomenon, select a wherein seriously ill piglet, the mucus got with the blood streak in fresh excreta is a little, or get fritter and have the direct smear of the colorectal mucosa of obvious pathological changes, fix through flame after natural drying in atmosphere, with ammonium oxalate crystal violet liquid, Ji's nurse dyeing liquor dyes 4 minutes, after smear washing is dried in the shade, examine under a microscope, can see that swine dysentery is crawled spirillum, or above-mentioned pathological material of disease 1 droplet is placed on microscope slide, dripping 1 normal saline, mixing, then cover slide, with dark-field microscopy (400 times), when finding that there is the thalline vivaciously moved in Serpentis sample, swine dysentery can be diagnosed as.Place is raised immediately by not occurring that the piglet of symptom isolates.Choose 50 ill piglets wherein, start to feed and be added with the feedstuff of the embodiment of the present invention 2 powder, powder is mixed in conventional feed according to 5g:500g weight ratio, continuous nursing 6 days, within 1st day, ill piglet symptom is obviously improved, and within the 2nd day, 50 piglets are all fully recovered, after continuing to feed 4 days, compared with sodium selenite, average weight is substantially identical.

The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims

1. treat the pharmaceutical composition of piglet diarrhea for one kind, it is characterized in that, described pharmaceutical composition comprises following raw medicinal material: see blood live, halberd Ye Wawei, Cirsium souliei (Franch.) Mattf., alkali ground Chinese small iris, Radix Codonopsis, Radix Chloranthi Serrati, Bulbus Allii, Herba cuscutae, Semen Hoveniae (Fructus Hoveniae), Radix Et Rhizoma Rhei, bright and beautiful line boomerang, the Radix Pulsatillae, Herba Portulacae, Radix Sanguisorbae, Rhizoma Cimicifugae, Radix Tinosporae, Semen Euryales, Hemsleya sphaerocarpa Kuang et A. M. Lu, Folium Isatidis, Flos Sophorae, Herba Potentillae Chinensis, Mel tank, powder Rhizoma Smilacis Chinensis, Climbing Viloet Herb, Cortex Moutan, Herba Verbenae, Herba Pteridis Multifidae, Herba panici repentics, Herba Violae, Indigo Naturalis, Rhizoma Imperatae, ten calyx eggplant and Balaenoptera borealis Lessons.

2. the pharmaceutical composition for the treatment of piglet diarrhea according to claim 1, is characterized in that, in described pharmaceutical composition, the ratio of weight and number of each raw medicinal material is: see that blood lives 10-35 weight portion, halberd Ye Wawei 12-22 weight portion, Cirsium souliei (Franch.) Mattf. 10-30 weight portion, alkali ground Chinese small iris 11-31 weight portion, Radix Codonopsis 14-28 weight portion, Radix Chloranthi Serrati 15-35 weight portion, Bulbus Allii 15-25 weight portion, Herba cuscutae 13-33 weight portion, Semen Hoveniae (Fructus Hoveniae) 11-21 weight portion, Radix Et Rhizoma Rhei 12-26 weight portion, brocade line boomerang 14-36 weight portion, Radix Pulsatillae 11-33 weight portion, Herba Portulacae 10-30 weight portion, Radix Sanguisorbae 13-33 weight portion, Rhizoma Cimicifugae 10-20 weight portion, Radix Tinosporae 13-27 weight portion, Semen Euryales 14-34 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 11-32 weight portion, Folium Isatidis 10-30 weight portion, Flos Sophorae 12-36 weight portion, Herba Potentillae Chinensis 10-28 weight portion, Mel tank 14-36 weight portion, powder Rhizoma Smilacis Chinensis 10-20 weight portion, Climbing Viloet Herb 12-24 weight portion, Cortex Moutan 10-20 weight portion, Herba Verbenae 11-23 weight portion, Herba Pteridis Multifidae 12-32 weight portion, Herba panici repentics 15-35 weight portion, Herba Violae 12-24 weight portion, Indigo Naturalis 13-33 weight portion, Rhizoma Imperatae 12-31 weight portion, ten calyx eggplant 11-29 weight portions and Balaenoptera borealis Lesson 14-35 weight portion.

3. the pharmaceutical composition for the treatment of piglet diarrhea according to claim 1 and 2, is characterized in that, in described pharmaceutical composition, the ratio of weight and number of each raw medicinal material is: see that blood lives 33 weight portions, halberd Ye Wawei 20 weight portion, Cirsium souliei (Franch.) Mattf. 25 weight portion, alkali ground Chinese small iris 31 weight portion, Radix Codonopsis 18 weight portion, Radix Chloranthi Serrati 32 weight portion, Bulbus Allii 25 weight portion, Herba cuscutae 31 weight portion, Semen Hoveniae (Fructus Hoveniae) 21 weight portion, Radix Et Rhizoma Rhei 24 weight portion, brocade line boomerang 32 weight portion, the Radix Pulsatillae 31 weight portion, Herba Portulacae 28 weight portion, Radix Sanguisorbae 32 weight portion, Rhizoma Cimicifugae 16 weight portion, Radix Tinosporae 25 weight portion, Semen Euryales 32 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 30 weight portion, Folium Isatidis 30 weight portion, Flos Sophorae 34 weight portion, Herba Potentillae Chinensis 22 weight portion, Mel tank 32 weight portion, powder Rhizoma Smilacis Chinensis 20 weight portion, Climbing Viloet Herb 14 weight portion, Cortex Moutan 16 weight portion, Herba Verbenae 23 weight portion, Herba Pteridis Multifidae 32 weight portion, Herba panici repentics 28 weight portion, Herba Violae 22 weight portion, Indigo Naturalis 33 weight portion, Rhizoma Imperatae 30 weight portion, ten calyx eggplant 26 weight portions and Balaenoptera borealis Lesson 34 weight portion.

4. the pharmaceutical composition of the treatment piglet diarrhea according to any one of claim 1-3, is characterized in that, described pharmaceutical composition also comprises Radix Puerariae, Herb Polygoni Chinensis, Herba Dianthi and Fructus Bruceae.

5. the pharmaceutical composition of the treatment piglet diarrhea according to any one of claim 1-4, is characterized in that, in described pharmaceutical composition, the parts by weight of each raw medicinal material are: see that blood lives 10-35 weight portion, halberd Ye Wawei 12-22 weight portion, Cirsium souliei (Franch.) Mattf. 10-30 weight portion, alkali ground Chinese small iris 11-31 weight portion, Radix Codonopsis 14-28 weight portion, Radix Chloranthi Serrati 15-35 weight portion, Bulbus Allii 15-25 weight portion, Herba cuscutae 13-33 weight portion, Semen Hoveniae (Fructus Hoveniae) 11-21 weight portion, Radix Et Rhizoma Rhei 12-26 weight portion, brocade line boomerang 14-36 weight portion, Radix Pulsatillae 11-33 weight portion, Herba Portulacae 10-30 weight portion, Radix Sanguisorbae 13-33 weight portion, Rhizoma Cimicifugae 10-20 weight portion, Radix Tinosporae 13-27 weight portion, Semen Euryales 14-34 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 11-32 weight portion, Folium Isatidis 10-30 weight portion, Flos Sophorae 12-36 weight portion, Herba Potentillae Chinensis 10-28 weight portion, Mel tank 14-36 weight portion, powder Rhizoma Smilacis Chinensis 10-20 weight portion, Climbing Viloet Herb 12-24 weight portion, Cortex Moutan 10-20 weight portion, Herba Verbenae 11-23 weight portion, Herba Pteridis Multifidae 12-32 weight portion, Herba panici repentics 15-35 weight portion, Herba Violae 12-24 weight portion, Indigo Naturalis 13-33 weight portion, Rhizoma Imperatae 12-31 weight portion, ten calyx eggplant 11-29 weight portions, Balaenoptera borealis Lesson 14-35 weight portion, Radix Puerariae 13-23 weight portion, Herb Polygoni Chinensis 14-36 weight portion, Herba Dianthi 12-32 weight portion and Fructus Bruceae 3-5 weight portion.

6. the pharmaceutical composition of the treatment piglet diarrhea according to any one of claim 1-5, is characterized in that, in described pharmaceutical composition, the parts by weight of each raw medicinal material are: see that blood lives 33 weight portions, halberd Ye Wawei 20 weight portion, Cirsium souliei (Franch.) Mattf. 25 weight portion, alkali ground Chinese small iris 31 weight portion, Radix Codonopsis 18 weight portion, Radix Chloranthi Serrati 32 weight portion, Bulbus Allii 25 weight portion, Herba cuscutae 31 weight portion, Semen Hoveniae (Fructus Hoveniae) 21 weight portion, Radix Et Rhizoma Rhei 24 weight portion, brocade line boomerang 32 weight portion, the Radix Pulsatillae 31 weight portion, Herba Portulacae 28 weight portion, Radix Sanguisorbae 32 weight portion, Rhizoma Cimicifugae 16 weight portion, Radix Tinosporae 25 weight portion, Semen Euryales 32 weight portion, Hemsleya sphaerocarpa Kuang et A. M. Lu 30 weight portion, Folium Isatidis 30 weight portion, Flos Sophorae 34 weight portion, Herba Potentillae Chinensis 22 weight portion, Mel tank 32 weight portion, powder Rhizoma Smilacis Chinensis 20 weight portion, Climbing Viloet Herb 14 weight portion, Cortex Moutan 16 weight portion, Herba Verbenae 23 weight portion, Herba Pteridis Multifidae 32 weight portion, Herba panici repentics 28 weight portion, Herba Violae 22 weight portion, Indigo Naturalis 33 weight portion, Rhizoma Imperatae 30 weight portion, ten calyx eggplant 26 weight portions and Balaenoptera borealis Lesson 34 weight portion, Radix Puerariae 21 weight portion, Herb Polygoni Chinensis 34 weight portion, Herba Dianthi 30 weight portion and Fructus Bruceae 3 weight portion.

7. the pharmaceutical composition of the treatment piglet diarrhea according to any one of claim 1-6, is characterized in that, the dosage form of described pharmaceutical composition is injectable powder, and its preparation method comprises the following steps:

8. the pharmaceutical composition of the treatment piglet diarrhea according to any one of claim 1-6, is characterized in that, the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:

9. the pharmaceutical composition of the treatment piglet diarrhea according to any one of claim 1-6, is characterized in that, the dosage form of described pharmaceutical composition is injectable powder, and its preparation method comprises the following steps:

10. a preparation method for the pharmaceutical composition of the treatment piglet diarrhea as described in any one of claim 1-6, is characterized in that, the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps: