CN104829360A - Microbial fertilizer capable of improving plant stress resistance and preparation method thereof - Google Patents
Microbial fertilizer capable of improving plant stress resistance and preparation method thereof Download PDFInfo
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- CN104829360A CN104829360A CN201510226740.1A CN201510226740A CN104829360A CN 104829360 A CN104829360 A CN 104829360A CN 201510226740 A CN201510226740 A CN 201510226740A CN 104829360 A CN104829360 A CN 104829360A
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Abstract
The invention discloses a microbial fertilizer capable of improving the plant stress resistance and a preparation method thereof. The fertilizer is composed of the following components in parts by weight: 70 to 85 parts of edible fungus waste materials, 5 to 10 parts of EM inoculant, 8 to 14 parts of humic acid, 20 to 30 parts of lotus pool sludge, 25 to 40 parts of peanut cake, 6 to 10 parts of ferrous sulfate, 5 to 9 parts of chitosan, 15 to 20 parts of urea, 15 to 25 parts of ammonium sulfate, 8 to 15 parts of potassium permanganate, 12 to 18 parts of phosphor fertilizer, 4 to 5 parts of binder, and water. The organic ecological fertilizer can enhance the stress resistance performance of crops and is prepared by using the edible fungus waste materials which are rich in proteins. Moreover, the humic acid and peanut cake are added to increase the organic substance content; and the humic acid can be rapidly absorbed by crops in advance, so the provided fertilizer can be used to save the dying crops. The microbial fertilizer is efficient, green and environment-friendly.
Description
Technical field
The invention belongs to the Technology field of fertilizer, be specifically related to a kind of microbial fertilizer improving crop anti-adversity and preparation method thereof.
Background technology
Be main raw material with edible fungi leftovers in invention, making a kind ofly provides the carrier of nutrition for microorganism sustenance growth, edible fungi leftovers is the residue that plantation edible mushrooms is left over, general by cotton seed hulls, rice husk, rice brans etc. are made, owing to planting edible mushrooms, generally be rich in the residue of a large amount of edible mushrooms, as residual fragment or the root system of flat mushroom or mushroom, these are rich in proteins and glucide all, here the material of other rich cellulose is mixed as sawdust etc., grind into homogenate, for culturing micro-organisms bacterium, first ferment, the structure function Conversion of measurement units such as protein are made to become small-molecule substance, the material such as nitrogen and phosphorus, plant materials can be total to directly absorb, proved by test: the waste material of edible mushroom after fermentation, organic content reduces, the content of its nitrogen and phosphorus improves.Further, it or a kind of microbial fertilizer embedding microorganism, beneficial microorganism bacterium enters soil, greatly improves soil property, improves water conservation, fertilizer-preserving ability.
Potassium bichromate titrimetric method surveys the principle of soil organic matter content
Under 170-180 DEG C of condition, with the standard weight potassiumchromate-sulphuric acid soln little over amount, oxidation soil organic matter, the titration of remaining potassium bichromate standard ferrous sulfate, calculate organic carbon amounts by the gauge of institute's quota of expenditure ferrous sulfate, thus extrapolate organic content, its reaction formula is as follows:
2K
2Cr
2O
7+3C+8H
2SO
4→2K
2SO
4+2Cr
2(SO
4)
3+3CO
2+8H
2O
K
2Cr
2O
7+6FeSO
4+7H
2SO
4→K
2SO
4+Cr
2(SO
4)
3+3Fe
2(SO
4)
3+7H
2O
Use Fe
2+the remaining Cr of titration
2o
7 2-time, with o-phenanthrolin (C
2h
8n
2) be oxidation-reduction indicator, in titration process, the colourshifting process of indicator is as follows: orange based on potassium bichromate of solution during beginning, and now indicator under oxidative conditions, in light blue, covered by the orange of potassium bichromate, during titration, solution is gradually in green (Cr
3+), to becoming greyish-green close to during terminal.Work as Fe
2+during excessive half of solution, solution then becomes red-brown, represents that color arrives terminal.
Alkaline chitinase clears up the principle that ultraviolet spectrophotometry surveys total nitrogen content
Soil sample in the presence of catalyzer, with the vitriol oil disappear boil time, various organic compounds containing nitrogen, through the high-temperature decomposition reaction of complexity, is converted into ammonium nitrogen.In the alkaline medium of 120 DEG C ~ 124 DEG C, make oxygenant with Potassium Persulphate, nitrogenous compound in water sample can be converted into nitrate, simultaneously the oxidized decomposition of most of organism.Absorbance A is measured respectively in wavelength 220nm and 275nm place by ultraviolet spectrophotometry
220and A
275, use A=A
220-2A
275obtain correction absorbance A, calculated the content of total nitrogen by working curve.
M3 lixiviate-molybdenum antimony resistance colorimetric method surveys the principle of soil phosphorus content
0.2mol/L HOAc-0.25mol/L NH in M3 digestion agent
4nO
3define the strong buffer system of pH2.5, and lixiviate can go out the positively charged ion such as exchangeability K, Ca, Mg, Fe, Mn, Cu, Zn; 0.015mol/LNH
4f-0.013mol/L HNO
3controllable P is from the desorb Ca, Al, Fe inorganic phosphorous sources; 0.001mol/LEDTA can leach Chelating state Cu, Zn, Mn, Fe etc., and therefore, M3 digestion agent can extract the various nutrient elements such as effective phosphorus, potassium, calcium, magnesium, iron, manganese, copper, zinc, boron in soil simultaneously.
Summary of the invention
A kind of microbial fertilizer improving crop anti-adversity, it is characterized in that, be made up of the raw material of following weight part: waste material of edible mushroom 70 ~ 85, EM microbial inoculum 5 ~ 10, humic acid 8 ~ 14, lotus pool mud 20 ~ 30, peanut cake 25 ~ 40, ferrous sulfate 6 ~ 10, chitosan 5 ~ 9, urea 15 ~ 20, ammonium sulfate 15 ~ 25, potassium permanganate 8 ~ 15, phosphate fertilizer 12 ~ 18, binding agent 4 ~ 5 and appropriate water;
Described binding agent is made up of the raw material of following weight part: terra alba 5 ~ 7, glycerol 2 ~ 3, urea-formaldehyde resin 8 ~ 10, Walocel MT 20.000PV 2 ~ 4, sodium laurylsulfonate 1 ~ 2, industrial rapeseed oil 4 ~ 6, ethanol 2 ~ 5 and water 25 ~ 30; Preparation method is (1) mixed industrial rapeseed oil and sodium laurylsulfonate, is blended into water, stirs 20 ~ 30min uniformly solution for standby; (2) in reaction cylinder, mixing urea-formaldehyde resin and the emulsion described in (1), add terra alba, Walocel MT 20.000PV, glycerol and ethanol, be heated to 60 ~ 80 DEG C, stir under 300 ~ 400r/min heat-retaining condition and disperse 30 ~ 50min and carry out composite, finally uniformly emulsion.
Improve a preparation method for the microbial fertilizer of crop anti-adversity, it is characterized in that, comprise following step:
(1) waste material of edible mushroom put into shredder and be blended into the water of total amount 1 ~ 2.5 times, first soaking 5 ~ 10h, be then ground to homogenate;
(2) be added in the homogenate described in (1) after EM microbial inoculum being diluted 3 ~ 5 times, 40 ~ 60min is stirred under 300 ~ 500r/min normal temperature, put into the constant temperature culture room quiescent culture 20 ~ 27 days of 35 ~ 42 DEG C, every 12h stirs once, is suitably watered and keeps the uniform thickener of water content 80%;
(3) by binding agent thin up 3 ~ 5 times, be added in the fermentation material of (2), after stirring, logical hot-air seasoning process, becomes solid block after leaving standstill 24h, is ground into the granule of 40 mesh sieves;
(4) humic acid, lotus pool mud, peanut cake, ferrous sulfate, chitosan, urea, ammonium sulfate, potassium permanganate, phosphate fertilizer and other remaining component is mixed; be crushed to pulvis; then (3) gained granule is added; granulation after the adjustment water content 40% that adds water after abundant mixing; the particle of particle diameter 5 ~ 7mm is made, finally logical hot-air seasoning in rotary drum granulator.
By the thickener after the fermentation of invention step (2) gained, the edible mushrooms fertilizer of flat mushroom and mushroom is undertaken that it is organic, the assay of nitrogen and phosphorus, the concrete change that blank group detects each composition of waste material of edible mushroom before and after fermentation is set simultaneously, draws following data according to detection:
Dried respectively by sample, pulverize, after crossing aperture 0.25mm sieve, organic to sample, full nitrogen, full potassium, full phosphorus carry out analysis mensuration, and wherein organic mensuration adopts potassium bichromate titrimetric method---external heat; Determination of total nitrogen content adopts alkaline chitinase to clear up ultraviolet spectrophotometry; Full phosphorus measures and adopts M3 lixiviate-molybdenum antimony resistance colorimetric method.
Organic mensuration
Titration data
According to following formulae discovery organic content
In formula: V
0feS0 used when------titration is blank
4milliliter number (mean value);
FeS0 used during V------titration soil sample
4milliliter number;
5.00------K used
2cr
2o
7milliliter number
0.1333---K
2cr
20
7the concentration of standardized solution;
0.003---carbon mmole quality 0.012 by reaction in electron exchange number 4 remove 0.003;
1.724---organic carbon content average out to 58%, therefore the coefficient when carbon measured is converted into organic matter is 100/58 ≈ 1.724;
---1.1---correction coefficient.
Before and after fermentation, because subtilis decomposes the organic matter in two kinds of waste material of edible mushroom, the organic content of two kinds of waste material of edible mushroom obviously reduces.
The mensuration of total nitrogen
Nitrogen content before and after two kinds of fermentation waste
Before and after fermentation, because subtilis decomposes the organic matter in two kinds of waste material of edible mushroom, generate nitrogenous compound, the nitrogen content of two kinds of waste material of edible mushroom obviously increases.
The mensuration of phosphorus content
Standard P spectrophotometric value
Soil M3-P (mg/L or mg/kg)=[ρ (P) × V × D]/[V
0or (M)]
In formula:
ρ---P concentration in liquid to be measured, mg/L;
V---nitrite ion volume, 5.00mL;
D---divide and get multiple, leach liquor volume/absorption filtrate volume;
V
0(or M)---soil sample volume, mL or quality of soil sample, g.
P content before and after two kinds of fermentation waste
Before and after fermentation, because subtilis decomposes the organic matter in two kinds of waste material of edible mushroom, generate P contained compound, phosphorus content obviously increases.
Because subtilis decomposes the organic matter in two kinds of waste material of edible mushroom, before and after fermentation, organic content reduces, nitrogenous, phosphorus, potassium compound increases, so the waste material of edible mushroom after fermentation can be used on the cash crop such as vegetables, melon and fruit, tealeaves, tobacco leaf, can effectively increase production.
Beneficial effect of the present invention: the present invention is by the utilization to the waste material of edible mushroom of rich in proteins, obtain a kind of ecological organic fertilizer improving crop anti-adversity, first by probiotics mixed edible bacterium fermentation waste, improve the various plants growth regulators such as organic acid, indolylacetic acid and Plant hormones regulators,gibberellins, improve the vitality of bacterial classification simultaneously, then add humic acid, peanut cake etc. and improve follow-up organism supply, humic acid has characteristic preferential absorbed by crops fast, so fertilizer of the present invention can also save the crop of being at death's door, efficient, green and environmental protection.
Accompanying drawing explanation
The linear regression curves broken line graph that Fig. 1 does with corresponding spectrophotometric value for the P concentration of leach liquor before and after two kinds of fermentation waste.
Embodiment
Embodiment 1:
A kind of microbial fertilizer improving crop anti-adversity, it is characterized in that, be made up of the raw material of following weight part (kg): waste material of edible mushroom 85, EM microbial inoculum 8, humic acid 8, lotus pool mud 24, peanut cake 30, ferrous sulfate 6, chitosan 6, urea 20, ammonium sulfate 15, potassium permanganate 10, phosphate fertilizer 15, binding agent 4 and appropriate water;
Described binding agent is made up of the raw material of following weight part: terra alba 6, glycerol 3, urea-formaldehyde resin 10, Walocel MT 20.000PV 3, sodium laurylsulfonate 1, industrial rapeseed oil 4, ethanol 4 and water 30; Preparation method is (1) mixed industrial rapeseed oil and sodium laurylsulfonate, is blended into water, stirs 20 ~ 30min uniformly solution for standby; (2) in reaction cylinder, mixing urea-formaldehyde resin and the emulsion described in (1), add terra alba, Walocel MT 20.000PV, glycerol and ethanol, be heated to 60 ~ 80 DEG C, stir under 300 ~ 400r/min heat-retaining condition and disperse 30 ~ 50min and carry out composite, finally uniformly emulsion.
Improve a preparation method for the microbial fertilizer of crop anti-adversity, it is characterized in that, comprise following step:
(1) waste material of edible mushroom put into shredder and be blended into the water of total amount 1 ~ 2.5 times, first soaking 5 ~ 10h, be then ground to homogenate;
(2) be added in the homogenate described in (1) after EM microbial inoculum being diluted 3 ~ 5 times, 40 ~ 60min is stirred under 300 ~ 500r/min normal temperature, put into the constant temperature culture room quiescent culture 20 ~ 27 days of 35 ~ 42 DEG C, every 12h stirs once, is suitably watered and keeps the uniform thickener of water content 80%;
(3) by binding agent thin up 3 ~ 5 times, be added in the fermentation material of (2), after stirring, logical hot-air seasoning process, becomes solid block after leaving standstill 24h, is ground into the granule of 40 mesh sieves;
(4) humic acid, lotus pool mud, peanut cake, ferrous sulfate, chitosan, urea, ammonium sulfate, potassium permanganate, phosphate fertilizer and other remaining component is mixed; be crushed to pulvis; then (3) gained granule is added; granulation after the adjustment water content 40% that adds water after abundant mixing; the particle of particle diameter 5 ~ 7mm is made, finally logical hot-air seasoning in rotary drum granulator.
The microbial fertilizer of raising crop anti-adversity of the present invention is used for plant common agricultural experiment, select 5 mu of planting bases, use fertilizer of the present invention, as experimental group, common fertilizer is executed in 5 mu of bases of control group routinely planting requirement, other condition is identical, experiment proves, when using identical amount fertilizer, the farm crop of experimental group plantation, its stalk and branches and leaves more sturdy, growing way is obviously better than control group, output obviously promotes, save labour simultaneously, protection of the environment, significant role is played in prolongation crop yield time and disease and insect resistance, economy comparatively improves 22.7% former years.
Claims (2)
1. one kind is improved the microbial fertilizer of crop anti-adversity, it is characterized in that, be made up of the raw material of following weight part: waste material of edible mushroom 70 ~ 85, EM microbial inoculum 5 ~ 10, humic acid 8 ~ 14, lotus pool mud 20 ~ 30, peanut cake 25 ~ 40, ferrous sulfate 6 ~ 10, chitosan 5 ~ 9, urea 15 ~ 20, ammonium sulfate 15 ~ 25, potassium permanganate 8 ~ 15, phosphate fertilizer 12 ~ 18, binding agent 4 ~ 5 and appropriate water;
Described binding agent is made up of the raw material of following weight part: terra alba 5 ~ 7, glycerol 2 ~ 3, urea-formaldehyde resin 8 ~ 10, Walocel MT 20.000PV 2 ~ 4, sodium laurylsulfonate 1 ~ 2, industrial rapeseed oil 4 ~ 6, ethanol 2 ~ 5 and water 25 ~ 30; Preparation method is (1) mixed industrial rapeseed oil and sodium laurylsulfonate, is blended into water, stirs 20 ~ 30min uniformly solution for standby; (2) in reaction cylinder, mixing urea-formaldehyde resin and the emulsion described in (1), add terra alba, Walocel MT 20.000PV, glycerol and ethanol, be heated to 60 ~ 80 DEG C, stir under 300 ~ 400r/min heat-retaining condition and disperse 30 ~ 50min and carry out composite, finally uniformly emulsion.
2. a kind of preparation method improving the microbial fertilizer of crop anti-adversity according to claim 1, is characterized in that, comprise following step:
(1) waste material of edible mushroom put into shredder and be blended into the water of total amount 1 ~ 2.5 times, first soaking 5 ~ 10h, be then ground to homogenate;
(2) be added in the homogenate described in (1) after EM microbial inoculum being diluted 3 ~ 5 times, 40 ~ 60min is stirred under 300 ~ 500r/min normal temperature, put into the constant temperature culture room quiescent culture 20 ~ 27 days of 35 ~ 42 DEG C, every 12h stirs once, is suitably watered and keeps the uniform thickener of water content 80%;
(3) by binding agent thin up 3 ~ 5 times, be added in the fermentation material of (2), after stirring, logical hot-air seasoning process, becomes solid block after leaving standstill 24h, is ground into the granule of 40 mesh sieves;
(4) humic acid, lotus pool mud, peanut cake, ferrous sulfate, chitosan, urea, ammonium sulfate, potassium permanganate, phosphate fertilizer and other remaining component is mixed; be crushed to pulvis; then (3) gained granule is added; granulation after the adjustment water content 40% that adds water after abundant mixing; the particle of particle diameter 5 ~ 7mm is made, finally logical hot-air seasoning in rotary drum granulator.
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| CN201510226740.1A CN104829360A (en) | 2015-05-06 | 2015-05-06 | Microbial fertilizer capable of improving plant stress resistance and preparation method thereof |
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| CN201510226740.1A CN104829360A (en) | 2015-05-06 | 2015-05-06 | Microbial fertilizer capable of improving plant stress resistance and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105175110A (en) * | 2015-08-19 | 2015-12-23 | 颍上县小张庄农业科技有限公司 | Conditioning fertilizer for green pepper in Yingshang |
| CN105439710A (en) * | 2015-12-11 | 2016-03-30 | 马鞍山科邦生态肥有限公司 | Microbial fertilizer by efficient utilization of editable fungi residue and preparation method thereof |
| CN105481543A (en) * | 2015-12-10 | 2016-04-13 | 马鞍山科邦生态肥有限公司 | Organic fertilizer used for plantation of leek crops in barren lands, and preparation method thereof |
| CN106717902A (en) * | 2016-12-13 | 2017-05-31 | 张学知 | A kind of arable farming method carried out using waste product |
| CN107722988A (en) * | 2017-10-25 | 2018-02-23 | 湖南望隆企业管理咨询有限公司 | One heavy metal species soil-repairing agent and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2037730A (en) * | 1978-12-14 | 1980-07-16 | Genoud E | Fertilizer from fermented organic residues, manure, and peat |
| CN1309110A (en) * | 2001-02-16 | 2001-08-22 | 杨雄 | Process for preparing organic fertilizer from dungs and droppings |
| CN102643120A (en) * | 2011-02-17 | 2012-08-22 | 潍坊市云涛有机肥料有限公司 | Preparation method of organic fertilizer |
-
2015
- 2015-05-06 CN CN201510226740.1A patent/CN104829360A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2037730A (en) * | 1978-12-14 | 1980-07-16 | Genoud E | Fertilizer from fermented organic residues, manure, and peat |
| CN1309110A (en) * | 2001-02-16 | 2001-08-22 | 杨雄 | Process for preparing organic fertilizer from dungs and droppings |
| CN102643120A (en) * | 2011-02-17 | 2012-08-22 | 潍坊市云涛有机肥料有限公司 | Preparation method of organic fertilizer |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105175110A (en) * | 2015-08-19 | 2015-12-23 | 颍上县小张庄农业科技有限公司 | Conditioning fertilizer for green pepper in Yingshang |
| CN105481543A (en) * | 2015-12-10 | 2016-04-13 | 马鞍山科邦生态肥有限公司 | Organic fertilizer used for plantation of leek crops in barren lands, and preparation method thereof |
| CN105439710A (en) * | 2015-12-11 | 2016-03-30 | 马鞍山科邦生态肥有限公司 | Microbial fertilizer by efficient utilization of editable fungi residue and preparation method thereof |
| CN106717902A (en) * | 2016-12-13 | 2017-05-31 | 张学知 | A kind of arable farming method carried out using waste product |
| CN107722988A (en) * | 2017-10-25 | 2018-02-23 | 湖南望隆企业管理咨询有限公司 | One heavy metal species soil-repairing agent and preparation method thereof |
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Application publication date: 20150812 |
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