CN104781420A - 纯化核酸的方法及试剂盒 - Google Patents

纯化核酸的方法及试剂盒 Download PDF

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Publication number
CN104781420A
CN104781420A CN201380056277.XA CN201380056277A CN104781420A CN 104781420 A CN104781420 A CN 104781420A CN 201380056277 A CN201380056277 A CN 201380056277A CN 104781420 A CN104781420 A CN 104781420A
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nucleic acid
combination
liquid
mutually
purified
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李莉
李永梅
府宇雷
程亮
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Emerther Co
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Emerther Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明公开了一种纯化核酸的方法及试剂盒。具体地，本发明公开了一种可用于从包含核酸的样本中纯化核酸的试剂组合，基于该试剂组合制成的试剂盒，以及用该试剂组合或试剂盒纯化核酸的方法。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201380056277.XA 2012-10-26 2013-10-25 纯化核酸的方法及试剂盒 Pending CN104781420A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201380056277.XA CN104781420A (zh) 2012-10-26 2013-10-25 纯化核酸的方法及试剂盒

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201210418994.XA CN103789297A (zh) 2012-10-26 2012-10-26 快速纯化核酸的方法及试剂盒
CN201210418994X 2012-10-26
CN201380056277.XA CN104781420A (zh) 2012-10-26 2013-10-25 纯化核酸的方法及试剂盒
PCT/CN2013/085968 WO2014063651A1 (zh) 2012-10-26 2013-10-25 纯化核酸的方法及试剂盒

Publications (1)

Publication Number Publication Date
CN104781420A true CN104781420A (zh) 2015-07-15

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CN201380056277.XA Pending CN104781420A (zh) 2012-10-26 2013-10-25 纯化核酸的方法及试剂盒

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US (1) US20150275269A1 (zh)
CN (2) CN103789297A (zh)
WO (1) WO2014063651A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
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CN105670915A (zh) * 2016-04-05 2016-06-15 苏州英芮诚生化科技有限公司 磁珠预分装采血管及其对血液dna的预处理工艺

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GB2531741A (en) * 2014-10-28 2016-05-04 Bisn Laboratory Services Ltd Molecular and bioinformatics methods for direct sequencing
EP3230447A1 (en) * 2014-12-09 2017-10-18 Corning Incorporated Methods and kits for purifying plasmid dna
WO2018008032A1 (en) * 2016-07-07 2018-01-11 Technion Research & Development Foundation Ltd. Methods for isolation and quantification of short nucleic acid molecules
CN106906207A (zh) * 2017-04-19 2017-06-30 遵义医学院 核酸快速提取试剂盒及其使用方法
WO2019035335A1 (ja) * 2017-08-18 2019-02-21 Ａｇｃ株式会社 核酸の回収方法、核酸結合用担体および核酸回収キット
CN107779451A (zh) * 2017-11-20 2018-03-09 广州海思医疗科技有限公司 一种人类游离dna提取方法及其试剂盒
CN113195714A (zh) * 2018-08-17 2021-07-30 塞弗德公司 核酸分离的方法和组合物
CN112980830B (zh) * 2019-12-18 2023-06-20 深圳华大智造科技股份有限公司 一种用于磁珠法核酸提取的试剂盒、磁珠及其制备方法
CN111534511A (zh) * 2020-06-03 2020-08-14 沈阳德宇生物科技有限公司 用于核酸纯化回收的反应液及其核酸回收试剂盒和应用
CN111607635B (zh) * 2020-06-23 2024-03-22 大连理工大学 一种基于3d打印特形功能体的血液基因组dna提取方法及其应用试剂盒
CN111876410A (zh) * 2020-08-13 2020-11-03 武汉生之源生物科技股份有限公司 一种基因组柱提取试剂盒和提取方法
CN112321870B (zh) * 2020-11-06 2022-11-18 浙江工商大学 一种微针贴片及其制备方法和应用
CN113355320A (zh) * 2021-01-25 2021-09-07 汉远化生医国际科技(北京)有限公司 用于分离和/或纯化核酸的裂解、结合、洗涤和/或洗脱试剂
CN112899266A (zh) * 2021-02-04 2021-06-04 北京中科医学检验实验室有限公司 一种核酸提取用裂解结合液及其试剂盒和应用
CN112941066B (zh) * 2021-02-04 2023-05-05 廊坊诺道中科医学检验实验室有限公司 一种细菌核酸提取用裂解结合液及其试剂盒和应用
CN113265398A (zh) * 2021-06-18 2021-08-17 汉远化生医国际科技(北京)有限公司 一种绿色快速动物组织dna提取方法
CN114350649A (zh) * 2021-12-02 2022-04-15 力因精准医疗产品（上海）有限公司 一种核酸提取试剂盒及核酸提取方法
CN114480372B (zh) * 2022-03-10 2023-04-07 河北三狮生物科技有限公司 一种通用型核酸提取试剂盒及核酸提取方法
CN115058491A (zh) * 2022-06-30 2022-09-16 南京瑞贝西生物科技有限公司 一种基于磁珠法的引物快速脱盐纯化的方法
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
CN117143963B (zh) * 2023-10-30 2024-02-27 北京百力格生物科技有限公司 乙醇-乙酸钠法回收pcr产物中核酸的方法

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CN101684138A (zh) * 2008-09-26 2010-03-31 上海裕隆生物科技有限公司 一种应用纳米磁珠纯化核酸的试剂盒

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CN101023171A (zh) * 2004-08-02 2007-08-22 根特拉体系股份有限公司 采用固相支持物纯化dna的制剂和方法
CN101684138A (zh) * 2008-09-26 2010-03-31 上海裕隆生物科技有限公司 一种应用纳米磁珠纯化核酸的试剂盒
CN101613696A (zh) * 2009-08-05 2009-12-30 公安部物证鉴定中心 提取纯化dna的试剂

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105670915A (zh) * 2016-04-05 2016-06-15 苏州英芮诚生化科技有限公司 磁珠预分装采血管及其对血液dna的预处理工艺

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CN103789297A (zh) 2014-05-14
WO2014063651A1 (zh) 2014-05-01

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