CN104781394B - 用于培养间充质干细胞的方法 - Google Patents

用于培养间充质干细胞的方法 Download PDF

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CN104781394B
CN104781394B CN201380045750.4A CN201380045750A CN104781394B CN 104781394 B CN104781394 B CN 104781394B CN 201380045750 A CN201380045750 A CN 201380045750A CN 104781394 B CN104781394 B CN 104781394B
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cells
stem cells
mesenchymal stem
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cell therapy
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CN104781394A (zh
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梁允瑄
吴元
吴元一
权纯载
李美娟
田洪培
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    • C12N5/0602—Vertebrate cells
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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CN201380045750.4A 2012-09-03 2013-09-02 用于培养间充质干细胞的方法 Active CN104781394B (zh)

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KR10-2012-0097193 2012-09-03
KR1020120097193A KR101532556B1 (ko) 2012-09-03 2012-09-03 간엽줄기세포의 배양 방법
PCT/KR2013/007891 WO2014035215A1 (en) 2012-09-03 2013-09-02 Method for culturing mesenchymal stem cells

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Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3140417B1 (en) * 2014-05-09 2021-04-21 Reelabs Private Limited Foetal polymix of mesenchymal stem cells under hypoxic conditions for the treatment of clinical disorders
JP6519727B2 (ja) * 2014-09-17 2019-05-29 国立大学法人山口大学 間葉系幹細胞の培養方法、および肝機能障害用治療剤の製造方法
US11000694B2 (en) 2014-10-24 2021-05-11 The Board Of Trustees Of The University Of Illinois Use of lasers for treating and reversing fibrosis
CN107406829A (zh) * 2014-10-29 2017-11-28 R生物有限公司 培养干细胞的培养基组合物
TWI740812B (zh) * 2015-01-05 2021-10-01 國立陽明大學 用於治療肝臟疾病的間葉幹細胞
CN107849533A (zh) * 2015-02-20 2018-03-27 洪士杰 间充质干细胞用于治疗骨关节炎的用途
EP3299023A4 (en) * 2015-05-21 2019-03-20 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences OXYGEN ARM-TREATED MESENCHYMM STEM CELL AND ITS USE
CN109072190A (zh) * 2016-03-09 2018-12-21 蔚山大学校产学协力团 将jak1抑制剂作为有效成分包含的用于干细胞的增殖、分化促进或老化抑制的组成物
KR102447145B1 (ko) * 2016-03-31 2022-09-23 샌바이오, 인크. 줄기 세포 배양 및 치료를 위한 배지, 방법, 세포 및 분비 인자
EP3634438A4 (en) * 2017-06-05 2021-03-03 The Regents of The University of California COMPOSITIONS FOR THE TREATMENT OF NETWORK DISEASES AND METHOD FOR THEIR MANUFACTURE AND USE
JP7173007B2 (ja) 2017-06-27 2022-11-16 味の素株式会社 リボフラビン誘導体含有培地
CN107384858A (zh) * 2017-08-17 2017-11-24 成都康景生物科技有限公司 一种缺氧耐受型间充质干细胞的制备方法及其应用
CN109666631A (zh) * 2017-10-17 2019-04-23 澳门大学 增强干细胞活性的培养方法、应用以及用于治疗中枢神经疾病的制剂、制备方法及治疗方法
CN109706115B (zh) * 2017-10-26 2023-05-30 中国医科大学 一种小鼠骨髓间充质干细胞细胞系的构建方法
CN108359637B (zh) * 2018-02-14 2021-09-28 浙江生创精准医疗科技有限公司 一种宫血干细胞快速扩增的方法
CN109517786B (zh) * 2018-10-18 2022-06-17 广州元帅生物科技有限公司 一种具有修复功能的多能干细胞制剂及其应用
US20220348880A1 (en) * 2019-08-30 2022-11-03 The Texas A&M University System Xenogen-Free Mesenchymal Stem Cell Compositions and Methods of Use
WO2022004938A1 (ko) * 2020-06-30 2022-01-06 주식회사 미래셀바이오 유사 중간엽 줄기세포의 제조방법
CN112147340B (zh) * 2020-09-30 2024-03-08 北京银丰鼎诚生物工程技术有限公司 一种神经干细胞免疫调节功能的检测方法
CN112375733B (zh) * 2020-11-03 2022-09-02 中国人民解放军陆军军医大学第一附属医院 可增进间充质干细胞干细胞性的植物血凝素及其在间充质干细胞扩增培养中的应用
KR102435452B1 (ko) * 2020-11-11 2022-09-07 이엔셀 주식회사 노화가 감소되고 줄기세포능이 보존된 초기 중간엽 줄기세포, 및 그 배양방법
CN113502261B (zh) * 2021-07-16 2023-08-29 上海东方星际干细胞科技有限公司 一种能高效分化成脂的规模化3d低氧间充质干细胞培养体系
CN116218770B (zh) * 2022-12-30 2023-11-24 苏州科为康生物医药科技有限公司 一种间充质干细胞的制备方法和应用
CN117660325B (zh) * 2024-01-31 2024-04-19 苏州科为康生物医药科技有限公司 一种制备脐带血msc的培养基及其方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2039758A1 (en) * 2007-09-21 2009-03-25 Universität Leipzig Cell culture medium and method for culturing stem cells and progenitor cells
WO2010150094A1 (en) * 2009-06-26 2010-12-29 Life & Light Limited Mesenchymal stem cells grown under hypoxic conditions: compositions, methods and uses therefor

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE312615T1 (de) 2001-08-14 2005-12-15 Medipost Co Ltd Zusammensetzung zur behandlung von schäden des gelenkknorpels
US20030143207A1 (en) * 2001-10-18 2003-07-31 Livesey Stephen A. Remodeling of tissues and organ
JP2005517441A (ja) 2002-02-19 2005-06-16 メディポスト・カンパニー・リミテッド 臍帯血由来の間葉幹細胞・前駆細胞の分離培養方法及び間葉組織への分化誘導方法
US20050281788A1 (en) * 2002-07-30 2005-12-22 Cosimo De Bari Compositions comprising muscle progenitor cells and uses thereof
MXPA05013115A (es) * 2003-06-06 2006-05-25 Wyeth Corp Metodos y materiales para identificar agentes que modulan la remodelacion de hueso y agentes identificados por los mismos.
JP2006055106A (ja) * 2004-08-23 2006-03-02 National Institute Of Advanced Industrial & Technology ヒト血清培地を用いるヒト骨髄由来間葉系幹細胞培養法
PT2471907T (pt) * 2005-12-29 2019-01-28 Celularity Inc Populações de células estaminais placentárias
US20070178073A1 (en) * 2006-02-01 2007-08-02 Samsung Life Public Welfare Foundation Composition Comprising Separated or Proliferated Cells from Umbilical Cord Blood for Treating Developmental and/or Chronic Lung Disease
ME01562B (me) * 2007-02-12 2014-09-20 Anthrogenesis Corp Liječenje protuupalnih bolesti putem matičnih stanica posteljice
US8114668B2 (en) * 2007-05-14 2012-02-14 Cardiac Pacemakers, Inc. Composition for cold storage of stem cells
WO2009014272A1 (en) * 2007-07-20 2009-01-29 Dongguk University Industry-Academic Cooperation Foundation Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
GB0715648D0 (en) * 2007-08-10 2007-09-19 Cambridge Silicon Radio Ltd Channel decoding using similarity matching
WO2011043629A2 (ko) 2009-10-08 2011-04-14 서울대학교병원 인간 지방 줄기세포의 고활성을 유도하는 방법 및 배지
WO2012018040A1 (ja) * 2010-08-03 2012-02-09 北海道公立大学法人札幌医科大学 自己血清添加骨髄細胞培養システム、自己血清添加骨髄細胞培養方法および自己血清添加培養骨髄細胞を有効成分とする医薬組成物の製造方法
US20120052577A1 (en) * 2010-08-31 2012-03-01 The Regents Of The University Of California Culture system for stem cell propagation and neural and oligodendrocyte specification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2039758A1 (en) * 2007-09-21 2009-03-25 Universität Leipzig Cell culture medium and method for culturing stem cells and progenitor cells
WO2010150094A1 (en) * 2009-06-26 2010-12-29 Life & Light Limited Mesenchymal stem cells grown under hypoxic conditions: compositions, methods and uses therefor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Effect of the calcium sensing receptor on rat bone marrow-derived mesenchymal stem cell proliferation through the ERK1/2 pathway;Zhihui Xu，et al;《Mol Biol Rep》;20120208;第39卷;7271–7279 *

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ES2755802T3 (es) 2020-04-23
KR101532556B1 (ko) 2015-06-30
US10294456B2 (en) 2019-05-21
EP2893001A4 (en) 2016-03-09
EP2893001A1 (en) 2015-07-15
DK2893001T3 (da) 2019-12-02
CA2884000A1 (en) 2014-03-06
IN2015DN01737A (cg-RX-API-DMAC7.html) 2015-05-29
WO2014035215A1 (en) 2014-03-06
JP6478243B2 (ja) 2019-03-06
US20190218517A1 (en) 2019-07-18
JP2015532596A (ja) 2015-11-12
US11332715B2 (en) 2022-05-17
EP2893001B1 (en) 2019-10-30
US20150284686A1 (en) 2015-10-08
PT2893001T (pt) 2019-12-09
CN104781394A (zh) 2015-07-15
PL2893001T3 (pl) 2020-03-31
AU2013309642B2 (en) 2018-10-25
CA2884000C (en) 2021-08-24
US9580687B2 (en) 2017-02-28
KR20140030703A (ko) 2014-03-12
AU2013309642A1 (en) 2015-03-26
US20170191036A1 (en) 2017-07-06

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