CN104758193A - Chick embryo polypeptide mask and preparation method thereof - Google Patents
Chick embryo polypeptide mask and preparation method thereof Download PDFInfo
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- CN104758193A CN104758193A CN201510171834.3A CN201510171834A CN104758193A CN 104758193 A CN104758193 A CN 104758193A CN 201510171834 A CN201510171834 A CN 201510171834A CN 104758193 A CN104758193 A CN 104758193A
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Abstract
The invention relates to a chick embryo polypeptide mask and a preparation method thereof. The chick embryo polypeptide mask comprises the following raw material components in percentage by mass: 10-20% of chick embryo polypeptide powder, 1-2% of a thickener, 20-30% of a humectant, 10-20% of milk, 10-20% of pearl powder and 30-49% of deionized water. The preparation method comprises the following steps: breaking hulls of chick embryo eggs, crushing, performing ultrasonic treatment and enzymolysis, centrifuging, spraying and drying after completely drying to obtain the chick embryo polypeptide mask. The chick embryo polypeptide mask disclosed by the invention not only has the effects of resisting bacteria, delaying skin ageing and resisting oxidization, but also has the effects of whitening skin and moisturizing skin.
Description
Technical field
The present invention relates to a kind of Embryo Gallus domesticus polypeptide mask and preparation method thereof, belong to cosmetic products field.
Background technology
Chicken embryo fertilized eggs, through special hatching technique, makes the continuous growth promoter of embryo, the vital egg of tool, and Embryo Gallus domesticus a series of biology occurs in growth and development process and transforms, and is a kind of desirable Exogenous Oxygen Free Radicals remover.The long-term direct and extraneous contact of skin of face of human body, the elastic fiber of direct sunlight meeting coup injury deep skin and collagen protein, cause skin of face to become lax matt, occur wrinkle.
Embryo Gallus domesticus nutritional labeling contains protein, aminoacid, bioactive peptide, carbohydrate (glucose, fructose, galactose, arabinose, furanose etc.), lipid, fatty acid etc., makes chicken embryo have high nutrition and medical value.Wherein, Embryo Gallus domesticus polypeptide has more and enhances metabolism, improves immunity, slow down aging, the multiple important function such as antibacterial, has been widely used in health food, but its application in cosmetics is few.
Summary of the invention
Technical problem to be solved by this invention is the deficiency that exists for above-mentioned prior art and provides a kind of Embryo Gallus domesticus polypeptide mask and preparation method thereof, preparation technology is simple and convenient, active component enriches, and has the effect of antibacterial, delay skin aging, antioxidation and whitening and skin moistening.
The technical scheme that the problem that the present invention is the above-mentioned proposition of solution adopts is:
A kind of Embryo Gallus domesticus polypeptide mask, its raw material by mass percentage, comprises following component: Embryo Gallus domesticus polypeptide powder 10 ~ 20%, thickening agent 1 ~ 2%, wetting agent 20 ~ 30%, milk 10 ~ 20%, Margarita powder 10 ~ 20%, deionized water 30 ~ 49%.
By such scheme, described Embryo Gallus domesticus polypeptide powder be after chicken embryo is shelled through smashing to pieces, ultrasound wave, enzymolysis, lyophilization, spraying dry obtain.Wherein, described in smash to pieces be by except smashing pulping to pieces with tissue mashing machine after shell breaking, obtain chicken embryo virgin pulp liquid; Described ultrasound wave is pulverized at the vibrations of gained chicken embryo virgin pulp liquid, and temperature is approximately 40-50 DEG C, and the time is approximately 10-20min; The condition of described enzymolysis is: under pH is neutrallty condition, adopts papain to be incubated enzymolysis 5 ~ 6 hours at 50 ~ 60 DEG C; Described lyophilization be by enzymolysis after Embryo Gallus domesticus egg liquid carry out vacuum lyophilization, pre-freezing temperature is-(20-30) DEG C, drying time 12-20h, material thickness 6-10mm, obtains Embryo Gallus domesticus powdered egg water content≤4%; Described spray-dired condition is: inlet temperature about 160-180 DEG C, outlet temperature about 70-90 DEG C, feed rate is at about 440-480mL/h.
By such scheme, described thickening agent is Polyacrylate thickeners, substantially can be divided into two kinds: one is water miscible polyacrylate; Another kind is acrylic acid, the homopolymer of methacrylic acid or copolymer emulsion thickening agent.Such as, such commercially available thickening agent has carbomer 940, carbomer 934, carbomer 941.
By such scheme, described wetting agent is one or more mixture in any proportion in hyaluronic acid, D-panthenol, trehalose, Sorbitol, glycerol.
By such scheme, described wetting agent by mass percentage, comprises following component: hyaluronic acid 1 ~ 5%, D-panthenol 5 ~ 10%, trehalose 10 ~ 20%, Sorbitol 20 ~ 40%, glycerol 40 ~ 60%.
By such scheme, described milk is for adopting commercially available degreasing sterilization plain chocolate, fat content≤0.5%, protein content >=2.9%, non-fat solid >=8.1%.
By such scheme, described Margarita powder adopts nanometer Margarita powder, and granularity is less than 150,000 orders.
The preparation method of Embryo Gallus domesticus polypeptide mask provided by the invention, it comprises the steps:
(1) preparation of Embryo Gallus domesticus polypeptide powder: after chicken embryo is shelled through smashing to pieces, ultrasound wave, enzymolysis, lyophilization, spraying dry, obtain Embryo Gallus domesticus polypeptide powder; Embryo Gallus domesticus polypeptide powder, thickening agent, wetting agent, milk, Margarita powder, deionized water is taken according to the mass percent of component each in raw material;
(2) mixing of raw material: Embryo Gallus domesticus polypeptide powder, milk, Margarita powder is stand-by after being dissolved in a small amount of deionized water, obtains premixed liquid A; By thickening agent in the deionized water of surplus 70-80 DEG C swelling after, add wetting agent mix homogeneously, be cooled to 50-70 DEG C and add above-mentioned stand-by premixed liquid A, obtain Embryo Gallus domesticus polypeptide mask.
Compared with prior art, the invention has the beneficial effects as follows:
First, the present invention adopts and obtains after Embryo Gallus domesticus polypeptide powder by Embryo Gallus domesticus polypeptide through pretreatment, mix with other raw material (thickening agent, wetting agent, milk, Margarita powder, deionized water) again, the Embryo Gallus domesticus polypeptide mask of gained is made not only to have antibacterial, delay skin aging, antioxidation, but also there is the effect of whitening and skin moistening, its reason is to contain SOD activated protein in chicken embryo, has good stability, effectively can eliminate O
2 -and OH, play delay skin aging, antioxidative effect, and have strong inhibition effect to TYR enzymatic activity, can whitening effect, promote the glossiness of skin, and lysozyme wherein has antibacterial action;
Second; Embryo Gallus domesticus polypeptide mask of the present invention is a kind of cosmetics extracting the protection skin that water-soluble active polypeptides matter is prepared as effective active composition from chicken embryo; wetting agent is coordinated preferably to be combined by hyaluronic acid, D-panthenol, trehalose, Sorbitol, glycerol; make the Embryo Gallus domesticus polypeptide mask of gained still have excellent performance of keeping humidity after long-term preservation, preparation technology is simple and convenient, active component is abundant, effect is remarkable.
There is embodiment
Below in conjunction with embodiment, the present invention is described in further details, but content of the present invention is not limited in this.
In embodiment, hyaluronic acid is cosmetics-stage white powder, content>=99.3%; D-panthenol is water white transparency thick liquid, content>=99%, active substance content>=99%, not containing heavy metal; Trehalose is cosmetics-stage 80 order white powder, content>=99%, active substance content>=99%, content of beary metal≤0.02%; Sorbitol is food stage, content>=69%, and density is 1.305 (g/cm
3); Glycerol is pharmaceutical grade, content>=99.5%, and density is 1.2616 (g/cm
3); Milk is degreasing sterilization plain chocolate, fat content≤0.5%, protein content>=2.9%, non-fat solid>=8.1%; Margarita powder adopts nanometer Margarita powder, and granularity is less than 150,000 orders.
Embodiment 1
A kind of Embryo Gallus domesticus polypeptide mask, its raw material by mass percentage, comprises following component: Embryo Gallus domesticus polypeptide powder 10%, thickening agent carbomer 9401%, wetting agent 20%, milk 10%, Margarita powder 10%, deionized water 49%; Wherein said wetting agent by mass percentage, comprises following component: hyaluronic acid 2%, pantothenylol 10%, trehalose 20%, Sorbitol 28%, glycerol 40%.
The preparation method of above-mentioned Embryo Gallus domesticus polypeptide mask, it comprises the steps:
(1) preparation of Embryo Gallus domesticus polypeptide powder: the chicken embryo qualified alive choosing hatching non-broken shell in 14 days, carries out the sterilization of egg table, smashs 10min pulp except after shell breaking to pieces with tissue mashing machine, requires without naked eyes visible particulate thing, obtains chicken embryo virgin pulp liquid; Then pulverized by ultrasonic for chicken embryo virgin pulp liquid 40 DEG C 20min with ultrasound wave, transfer in hydrolysis reactor and stir with 700r/min, under temperature 50 C, pH adds papain enzymolysis 5h under 7.0 conditions, is filtered to below 400 orders; By enzymolysis filter after chicken embryo solution be-30 DEG C at vacuum freeze drier pre-freezing temperature, drying time 16h, material thickness 10mm, obtains Embryo Gallus domesticus powdered egg water content≤4%; Carry out spraying dry again, inlet temperature be 169.1 DEG C, outlet temperature 80.2 DEG C, feed rate 463.6mL/h, namely obtain Embryo Gallus domesticus polypeptide powder;
(2) take each component by proportioning described in the present embodiment raw material, by Embryo Gallus domesticus polypeptide powder, milk, that Margarita powder is dissolved in a small amount of deionized water is stand-by after temperature 50 C mix homogeneously, obtains premixed liquid A; Thickening agent carbomer 940 and wetting agent (hyaluronic acid, pantothenylol, trehalose, Sorbitol, glycerol) are placed in temperature be the balance deionized water of 80 DEG C swelling after, add on agitator that wetting agent low whipping speed is 3000r/min and fully stir 30min to dissolving mix homogeneously, be cooled to 50 DEG C and add above-mentioned stand-by premixed liquid A, obtain Embryo Gallus domesticus polypeptide mask.
Embodiment 2
A kind of Embryo Gallus domesticus polypeptide mask, its raw material by mass percentage, comprises following component: Embryo Gallus domesticus polypeptide powder 10%, carbomer 9341%, wetting agent 30%, milk 19%, Margarita powder 10%, deionized water 30%; Wherein said wetting agent by mass percentage, comprises following component: hyaluronic acid 5%, pantothenylol 5%, trehalose 10%, Sorbitol 20%, glycerol 60%.
The preparation method of above-mentioned Embryo Gallus domesticus polypeptide mask, it comprises the steps:
(1) preparation of Embryo Gallus domesticus polypeptide powder: the chicken embryo qualified alive choosing hatching non-broken shell in 14 days, carries out the sterilization of egg table, smashs 10min pulp except after shell breaking to pieces with tissue mashing machine, requires without naked eyes visible particulate thing, obtains chicken embryo virgin pulp liquid; Then pulverized by ultrasonic for chicken embryo virgin pulp liquid 50 DEG C 10min with ultrasound wave, transfer in hydrolysis reactor and stir with 900r/min, under temperature 60 C, pH adds papain enzymolysis 6h under 7.0 conditions, then uses 400 order filter-cloth filterings; After being filtered by enzymolysis, chicken embryo solution is-20 DEG C at-pre-freezing temperature, drying time 18h, material thickness 8mm, obtains Embryo Gallus domesticus powdered egg water content≤4%; Carry out spraying dry again, inlet temperature be 160.4 DEG C, outlet temperature 70.5 DEG C, feed rate 440.8mL/h, namely obtain Embryo Gallus domesticus polypeptide powder;
(2) take each component by proportioning described in the present embodiment raw material, by Embryo Gallus domesticus polypeptide powder, milk, that Margarita powder is dissolved in a small amount of deionized water is stand-by after temperature 50 C mix homogeneously, obtains premixed liquid A; Thickening agent carbomer 934 and wetting agent (hyaluronic acid, pantothenylol, trehalose, Sorbitol, glycerol) are placed in temperature be the balance deionized water of 70 DEG C swelling after, add on agitator that wetting agent low whipping speed is 2000r/min and fully stir 30min to dissolving mix homogeneously, be cooled to 60 DEG C and add above-mentioned stand-by premixed liquid A, obtain Embryo Gallus domesticus polypeptide mask.
Embodiment 3
A kind of Embryo Gallus domesticus polypeptide mask, its raw material by mass percentage, comprises following component: Embryo Gallus domesticus polypeptide powder 20%, carbomer 9411%, wetting agent 20%, milk 10%, Margarita powder 19%, deionized water 30%; Wherein, described wetting agent by mass percentage, comprises following component: hyaluronic acid 1%, pantothenylol 5%, trehalose 14%, Sorbitol 30%, glycerol 50%.
The preparation method of above-mentioned Embryo Gallus domesticus polypeptide mask, it comprises the steps:
(1) preparation of Embryo Gallus domesticus polypeptide powder: the chicken embryo qualified alive choosing hatching non-broken shell in 14 days, carries out the sterilization of egg table, smashs 15min pulp except after shell breaking to pieces with tissue mashing machine, requires without naked eyes visible particulate thing, obtains chicken embryo virgin pulp liquid; Then pulverized by ultrasonic for chicken embryo virgin pulp liquid 45 DEG C 15min with ultrasound wave, transfer in hydrolysis reactor and stir with 800r/min, under temperature 50 C, pH adds papain enzymolysis 6h under 7.0 conditions, is then filtered to below 400 orders; Pre-freezing temperature is-25 DEG C, drying time 12h, material thickness 6mm, obtains Embryo Gallus domesticus powdered egg water content≤4%; Carry out spraying dry again, inlet temperature be 180.0 DEG C, outlet temperature 90 DEG C, feed rate 480.7mL/h, namely obtain Embryo Gallus domesticus polypeptide powder;
(2) take each component by proportioning described in the present embodiment raw material, by Embryo Gallus domesticus polypeptide powder, milk, that Margarita powder is dissolved in a small amount of deionized water is stand-by after temperature 50 C mix homogeneously, obtains premixed liquid A; Thickening agent carbomer 941 and wetting agent (hyaluronic acid, pantothenylol, trehalose, Sorbitol, glycerol) are placed in temperature be the balance deionized water of 75 DEG C swelling after, add on agitator that wetting agent low whipping speed is 2500r/min and fully stir 30min to dissolving mix homogeneously, be cooled to 70 DEG C and add above-mentioned stand-by premixed liquid A, obtain Embryo Gallus domesticus polypeptide mask.
Measure of merit
Carry out result of use test to the Embryo Gallus domesticus polypeptide mask of embodiment 1-3 gained, and increase by two groups of controlled trials, wherein, matched group 1 is certain brand facial film commercially available; The formula of matched group 2 and the Embryo Gallus domesticus polypeptide mask difference of embodiment 1 are: do not add Embryo Gallus domesticus polypeptide powder, totally five groups of experiments, and result such as table 2 shows.
Result of use method of testing is: experimenter's recipient site skin is all without exception, does not also smear any external preparation, and dry after washing one's face, the 10min that sits quietly treats that the temperature of skin and environment reach balance, facial film is spread on face, washes away after 15min.
The tested crowd often organized is: in age 30-40 year, number 30 people, men and women half and half.Mass-send the facial film of answering of being rivals in a contest to respectively the often group experimenter, service time is 30 days, and fill in the unified questionnaire formulated after using and evaluate, evaluation result adopts average mark, and evaluation criterion is in table 1.
Table 1
Table 2
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Matched group 1 | Matched group 2 | |
| Skin irritation | 20 | 20 | 20 | 18 | 20 |
| Moisturizing degree | 18 | 19 | 20 | 12 | 16 |
| Defying age | 17 | 18 | 20 | 10 | 13 |
| Glossiness | 18 | 17 | 18 | 6 | 10 |
| Greasy degree | 20 | 20 | 18 | 9 | 18 |
In sum, Embryo Gallus domesticus polypeptide mask facial film of the present invention is without any zest and poison side effect, facial film good colour, homogeneous, clearly comfortable after application, without bad impression, be suitable for all kinds of skin, there is antibacterial, delay skin aging, antioxidation, and also have the effect of whitening and skin moistening, fundamentally improve facial skin quality, sense organ, physical and chemical index meet industry standard.
Although above-mentioned, the specific embodiment of the present invention is described; but not limiting the scope of the invention; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment that creative work can make or distortion still can in protection scope of the present invention.
Claims (10)
1. an Embryo Gallus domesticus polypeptide mask, the raw material that it is characterized in that it by mass percentage, comprises following component: Embryo Gallus domesticus polypeptide powder 10 ~ 20%, thickening agent 1 ~ 2%, wetting agent 20 ~ 30%, milk 10 ~ 20%, Margarita powder 10 ~ 20%, deionized water 30 ~ 49%.
2. a kind of Embryo Gallus domesticus polypeptide mask according to claim 1, it is characterized in that described Embryo Gallus domesticus polypeptide powder be after chicken embryo is shelled through smashing to pieces, ultrasound wave, enzymolysis, lyophilization, spraying dry obtain.
3. a kind of Embryo Gallus domesticus polypeptide mask according to claim 2, is characterized in that the condition of described enzymolysis is: under pH is neutrallty condition, adopts papain to be incubated enzymolysis 5 ~ 6 hours at 50 ~ 60 DEG C.
4. a kind of Embryo Gallus domesticus polypeptide mask according to claim 2, it is characterized in that described lyophilization be by enzymolysis after Embryo Gallus domesticus egg liquid carry out vacuum lyophilization, technological parameter is: pre-freezing temperature is-(20-30) DEG C, drying time 12-20h, material thickness 6-10mm, obtains Embryo Gallus domesticus powdered egg water content≤4%.
5. a kind of Embryo Gallus domesticus polypeptide mask according to claim 2, is characterized in that described spray-dired condition is: inlet temperature 160-180 DEG C, outlet temperature 70-90 DEG C, feed rate is at 440-480mL/h.
6. a kind of Embryo Gallus domesticus polypeptide mask according to claim 1, is characterized in that described thickening agent is polyacrylic ester thickener.
7. a kind of Embryo Gallus domesticus polypeptide mask according to claim 1, is characterized in that described wetting agent is one or more mixture in any proportion in hyaluronic acid, pantothenylol, trehalose, Sorbitol, glycerol.
8. a kind of Embryo Gallus domesticus polypeptide mask according to claim 2, it is characterized in that described wetting agent by mass percentage, comprise following component: hyaluronic acid 1 ~ 5%, pantothenylol 5 ~ 10%, trehalose 10 ~ 20%, Sorbitol 20 ~ 40%, glycerol 40 ~ 60%.
9. a kind of Embryo Gallus domesticus polypeptide mask according to claim 2, is characterized in that described milk adopts commercially available degreasing sterilization plain chocolate, fat content≤0.5%, protein content >=2.9%, non-fat solid >=8.1%; Described Margarita powder adopts nanometer Margarita powder, and granularity is less than 150,000 orders.
10. the preparation method of the Embryo Gallus domesticus polypeptide mask that one of claim 1-9 is described, is characterized in that it comprises the steps:
(1) preparation of Embryo Gallus domesticus polypeptide powder: after chicken embryo is shelled through smashing to pieces, ultrasound wave, enzymolysis, lyophilization, spraying dry, obtain Embryo Gallus domesticus polypeptide powder; Embryo Gallus domesticus polypeptide powder, thickening agent, wetting agent, milk, Margarita powder, deionized water is taken according to the mass percent of component each in raw material;
(2) mixing of raw material: Embryo Gallus domesticus polypeptide powder, milk, Margarita powder is stand-by after being dissolved in a small amount of deionized water, obtains premixed liquid A; After thickening agent is swelling in the deionized water of surplus, add wetting agent mix homogeneously, add above-mentioned stand-by premixed liquid A, obtain Embryo Gallus domesticus polypeptide mask.
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Cited By (7)
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| CN105380818A (en) * | 2015-12-30 | 2016-03-09 | 耿威 | Application of chick embryo bioactive peptide in preparing product for protecting cell activity |
| CN105411874A (en) * | 2015-12-30 | 2016-03-23 | 耿威 | Chick embryo bioactive peptide, preparation method and applications |
| CN105534849A (en) * | 2015-12-30 | 2016-05-04 | 耿威 | Chicken embryo peptide as well as preparation method and application thereof in preparation of anti-inflammation, acne removal and black hair generation products |
| CN105646647A (en) * | 2015-12-30 | 2016-06-08 | 耿威 | Chick embryo polypeptide as well as preparation method and application of chick embryo polypeptide |
| CN107114707A (en) * | 2017-05-14 | 2017-09-01 | 江西鹭中健康科技有限公司 | A kind of production technology of chicken embryo polypeptide powdered egg and application |
| CN107951739A (en) * | 2017-12-31 | 2018-04-24 | 山东禹泽医药科技有限公司 | A kind of small peptide combinations of chicken embryo with white-skinned face function |
| CN108743448A (en) * | 2018-08-30 | 2018-11-06 | 吉林省星空生物集团有限公司 | A kind of biological prosthetic facial mask and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105380818A (en) * | 2015-12-30 | 2016-03-09 | 耿威 | Application of chick embryo bioactive peptide in preparing product for protecting cell activity |
| CN105411874A (en) * | 2015-12-30 | 2016-03-23 | 耿威 | Chick embryo bioactive peptide, preparation method and applications |
| CN105534849A (en) * | 2015-12-30 | 2016-05-04 | 耿威 | Chicken embryo peptide as well as preparation method and application thereof in preparation of anti-inflammation, acne removal and black hair generation products |
| CN105646647A (en) * | 2015-12-30 | 2016-06-08 | 耿威 | Chick embryo polypeptide as well as preparation method and application of chick embryo polypeptide |
| CN105411874B (en) * | 2015-12-30 | 2018-11-20 | 耿威 | A kind of chicken embryo active peptide and the preparation method and application thereof |
| CN105534849B (en) * | 2015-12-30 | 2018-11-20 | 耿威 | A kind of chicken embryo peptide and preparation method thereof and the application in preparation anti-inflammatory, anti-acne, the regenerated product of dark hair |
| CN107114707A (en) * | 2017-05-14 | 2017-09-01 | 江西鹭中健康科技有限公司 | A kind of production technology of chicken embryo polypeptide powdered egg and application |
| CN107951739A (en) * | 2017-12-31 | 2018-04-24 | 山东禹泽医药科技有限公司 | A kind of small peptide combinations of chicken embryo with white-skinned face function |
| CN108743448A (en) * | 2018-08-30 | 2018-11-06 | 吉林省星空生物集团有限公司 | A kind of biological prosthetic facial mask and preparation method thereof |
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