CN104706963A - Preparation method of atomized liquid for preoperative intervention - Google Patents

Abstract

The invention discloses a preparation method of atomized liquid for preoperative intervention and aims to solve the problem of broncholithiasis treatment. The preparation method comprises the following steps: extracting volatile oil for standby use from leaves of Cunninghamia lanceolata (Lamb.) Hook., leaves of cannabis sativa L., Rabdosia sculponeata (Vaniot) Hara and Bulbophyllum inconspicuum Maxim. by steam distillation, and reserving residues; mixing adult Gryllotalpa africana Palisot et Beaurois (or adult G. unispina Saussure), root of small-leaf oriental grape, root of Rubus wilsonii Duthie, seeds of Carex baccans Nees, larval Holotrichia diomphalia Bates (or larval Anomala carpulenta Motsch.), Buchnera cruciatas Hamilt., root of Salix wallichiana Anders., seeds of Euryale ferox Salisb. and the residues obtained in step 1, and performing aqueous extraction to obtain an extract; concentrating the extract under reduced pressure, adding ethyl alcohol into the concentrated extract, keeping the concentrated extract standing under refrigeration, taking and filtering supernatant, recycling ethyl alcohol from filtrate, adding 0.5w% of powdered activated carbon into concentrated filtrate, boiling the concentrated filtrate for 30 minutes after uniform stirring, cooling the concentrated filtrate to 50DEG C, and performing suction filtration with a 0.4mu m filter membrane; and adding 0.2w% of potassium sorbate into suction filtrate, mixing the potassium sorbate and the suction filtrate uniformly, and spraying the volatile oil obtained in step 1 into the mixture of the potassium sorbate and the suction filtrate, uniformly mixing the volatile oil and the mixture, and performing subpackaging. Clinical experiments prove that the atomized liquid has the characteristics of good curative effect and high safety in broncholithiasis treatment and is worth application and popularization.

Description

A kind of preparation method of preoperative intervention atomized liquid

Technical field

The present invention relates to the field of Chinese medicines, be specifically related to the preparation method of intervening atomized liquid under a kind of tracheascope before lithotomy.

Background technology

Broncholithiasis refers to be positioned at bronchial lumen or the calcification agglomerate corroded to intracavity can cause serious bronchus stimulation and obstructive symptom.Previously research is thought, the lithogenous reason of shape mostly is: foreign body attracts or infects the lithogenous core of secretions structure, is progressively formed calculus by calcium phosphorus precipitation outward.Different from liver and bile stone and calculus of urethra, the calculus of broncholithiasis is attached to bronchial wall, and be difficult to mobile, therefore in existing expectant treatment, " diuretic calculus method " effect is unsatisfactory.Pathological study result in recent years shows, in the forming process of broncholithiasis, bronchial wall infiltrates a kind of basophilia inflammatory cluster of grains, and this kind of inflammatory cluster of grains has reunion characteristic, then stimulates cell infiltration and fibrosis, leads lithogenic final formation.And this kind " basophilia inflammatory cluster of grains " is the pathologic basis of broncholithiasis reunion attachment.

At present, there is no the specially good effect expectant treatment scheme for broncholithiasis, bronchial lavage thinks invalid more.Some patients can get stone by bronchoscope, but due to adhesion of reuniting because of calculus, if violence or tearing calculus, may meet accident in taking-up process, and therefore wherein have the patient of 68 ~ 81% to get stone failure, and carry out open chest surgery process, wound is huge.

In prior art, there is no the specific treatment scheme for broncholithiasis, bronchial lavage thinks invalid more.Some patients can get stone by bronchoscope.Due to adhesion of reuniting because of calculus, if violence or tearing calculus, may meet accident in taking-up process, therefore wherein have the patient of 68% to get stone failure, and carry out open chest surgery process, wound is huge.Pathological study result in recent years shows, in the forming process of broncholithiasis, bronchial wall infiltrates a kind of basophilia inflammatory cluster of grains, and this kind of inflammatory cluster of grains has reunion characteristic, then stimulates cell infiltration and fibrosis, leads lithogenic final formation.So use Chinese Traditional Medicine " fossil calculus " means, the treatment problem of broncholithiasis can not be solved.

Compared with prior art, the present invention has following characteristics:

1, get Gryllotalpa in prescription, smelly Herba Artemisiae is monarch drug: Gryllotalpa has very strong tissue penetration, walk to alter power strong, and it is little compared with other insect medicine for mucous membrane irritation, laboratory research shows that Gryllotalpa water logging agent (1:4) can make inflammatory cluster of grains loosen in vitro, and its mechanism of action is relevant with enzymolysis.Smelly Herba Artemisiae water extract 12.5mg/kg topical application all has obvious inhibitory action to Oleum Tiglii induced mice auricle acute exudative inflammation, and action intensity, higher than prednisolone acetate, also has the subcutaneous granulation tissue hyperplasia of cotton balls induced mice and shows inhibitory action.Gryllotalpa, smelly both Herba Artemisiaes coordinate the tack that effectively can reduce basophilia inflammatory cluster of grains, make existing " reunion " easier dismission, eliminate existing fiber laydown;

2, get Stachys geobombycis C. Y. Wu, Carex baccans Nees minister assistant Gryllotalpa, increase the penetration capacity of medicine, make it more easily be applied to bronchial wall basilar part, change to alleviate bronchial mucosa inflammation, antagonism bronchial wall cell infiltration and fibrosis;

3, get plumage arrow, Radix rubi pungentis, Folium Cunninghamiae lanceolatae, Salix wallichiana minister help smelly Herba Artemisiae heat-clearing and toxic substances removing; The sodium oxalacetate raised in plumage arrow decoct has the effect regulating extracellular environment abnormal metabolism; And the antagonism of its hemostatic capability prevents Drug Prepared from Insects from moving blood too;

4, Creeper root, Radix Salicis Wallichianae extract can reduce and ooze out and hypertrophy, contribute to preventing inflammatory granule from gathering further, recur probability after reducing treatment;

5, Gorgon fruit, Herba Bulbophylli inconspicui water decoction can reduce mucosa local excitation;

6, HUOMAYE has slight local anesthesia effect, alleviates the stimulation of Drug Prepared from Insects, and it can slight expansion bronchus;

7, medicine material consumption of the present invention is groped to sum up to draw through inventor in a large number, and each raw material dosage is for all to have good curative effect in following weight parts scope.

Summary of the invention

Technical assignment of the present invention is for above the deficiencies in the prior art, intervenes the preparation method of atomized liquid under the tracheascope providing a kind of curative effect to affirm before lithotomy.

The technical scheme that the present invention solves its technical problem is: a kind of inhalable aerosol drug for the treatment of broncholithiasis, it is characterized in that being prepared from by the crude drug of following weight proportion: Gryllotalpa 1 ~ 3 part, smelly Herba Artemisiae 3 ~ 12 parts, Stachys geobombycis C. Y. Wu 2 ~ 5 parts, Carex baccans Nees 3 ~ 12 parts, 12 ~ 20 parts, plumage arrow, Folium Cunninghamiae lanceolatae 10 ~ 15 parts, Radix rubi pungentis 12 ~ 20 parts, Salix wallichiana 12 ~ 20 parts, Creeper root 12 ~ 20 parts, Herba Bulbophylli inconspicui 3 ~ 12 parts, HUOMAYE 1 ~ 2 part, 10 ~ 25 parts, Gorgon fruit.

Chinese medicine of the present invention can be prepared from by following method:

(1) by Folium Cunninghamiae lanceolatae, HUOMAYE, smelly Herba Artemisiae, Herba Bulbophylli inconspicui extract volatile oil for subsequent use, medicinal residues are continued to employ;

(2) the medicinal residues mixing and water adding of Gryllotalpa, Creeper root, Radix rubi pungentis, Carex baccans Nees, Stachys geobombycis C. Y. Wu, plumage arrow, Salix wallichiana, Gorgon fruit and step 1 gained is decocted twice, each 1 hour, extracting solution filters, and merges;

(3) the extracting solution concentrating under reduced pressure of step 2 gained; Under agitation, in concentrated solution, add ethanol, under 0 ~ 4 DEG C of cold preservation, leave standstill 24 hours, get supernatant liquid filtering, filtrate recycling ethanol, and concentrated;

(4) step 3 gained concentrated filtrate by 0.5% weight add powdered activated carbon, boil 30 minutes after stirring evenly, when letting cool to 50 DEG C, with 0.4um filter membrane sucking filtration;

(5) step 4 gained sucking filtration liquid by 0.2% weight add potassium sorbate mix homogeneously after, spray into the volatile oil of step 1 gained, mixing, subpackage.

Wherein said: Gryllotalpa, for the adult of Gryllotalpidae insecticide Gryllotalpa Gryllotalpa africana Palisot et Beaurois or large Gryllotalpa G. unispina Saussure is all.Nature and flavor are salty, cold.Return bladder, large intestine, small intestine meridian.Function cures mainly: inducing diuresis to remove edema.

Smelly Herba Artemisiae is the herb of labiate Hemerocallis citrina Baroni Herba Rabdosiae glaucocalycis Rabdosia sculponeata (Vaniot) Hara.Acrid in the mouth; Warm in nature.Function cures mainly: regulate the flow of vital energy; Dampness removing; Removing toxic substances.

Stachys geobombycis C. Y. Wu, for Scarabeidae insecticide Korea scarab beetle Holotrichia diomphalia Bates or A. Corpulenta A-grade in the first class nomala carpulenta Motsch. is used as medicine with larva body.Salty, tepor.Function cures mainly: removing blood stasis with potent drugs blood, reducing swelling and alleviating pain, improving eyesight.

Carex baccans Nees is Cyperaceae carex Carex baccans Nees Carex baccans Nees seed.Sweet, pungent, flat.Function cures mainly: rash cough-relieving, invigorating middle warmer diuretic.

Plumage arrow is the herb of goatweed Ramulus Euonymi (Herba Buchnerae cruciatae) Buchnera cruciatas Hamilt..Lightly seasoned; Micro-hardship; Cool in nature.Function cures mainly: heat-clearing and toxic substances removing; Cooling blood for hemostasis.

Folium Cunninghamiae lanceolatae is the leaf of taxodiaceae plant China fir Cunninghamia lanceolata (Lamb.) Hook..Nature and flavor are pungent; Slightly warm in nature.Function cures mainly: dispel the wind; Reduce phlegm; Invigorate blood circulation; Removing toxic substances.

Radix rubi pungentis, is Rosaceae Radix rubi pungentis Rubus wilsonii Duthie, is used as medicine with root.Bitter, puckery, cool.Function cures mainly: pain relieving, antiinflammatory, regulating menstruation.

Salix wallichiana, is Salicaceae Salix wallichiana Salix wallichiana Anders., is used as medicine with root.Pungent, sour, puckery, cool.Function cures mainly: wind dispelling is antipyretic, dehumidifying.Cure mainly rheumatic arthritis.And wind syndrome of head pain can be controlled.

Creeper root is the root of vitaceae Pueraria lobota Bud.Nature and flavor are sweet, flat.Function cures mainly: nourishing qi and blood, reuniting the fractured tendons and bones, longue meat.

Herba Bulbophylli inconspicui is the orchid family Bulbophyllum platymiscium Herba Bulbophylli inconspicui Bulbophyllum inconspicuum Maxim., with all herbal medicine.Sweet, light, cool.Function cures mainly: nourishing YIN and clearing away heat, preventing phlegm from forming and stopping coughing.

HUOMAYE is the leaf of moraceae plants Fructus Cannabis Cannabis sativa L..Return lung; Bladder; Large intestine channel.Function cures mainly: preventing the attack (or recurrence) of malaria of relievining asthma; Removing toxic substances parasite killing.

Gorgon fruit is the dry mature kernal of nymphaeaceae plant Gorgon euryale Euryale ferox Salisb..Nature and flavor are sweet, puckery, flat.Gui Jing returns spleen, kidney channel.Function cures mainly: supplementing the kidney to control the nocturnal, spleen reinforcing antidiarrheal, damp eliminating leukorrhagia stopping.

Detailed description of the invention

Below in conjunction with practical situation, the specific embodiment of the present invention is elaborated.

Embodiment 1, crude drug weight proportion: Gryllotalpa 2g, smelly Herba Artemisiae 12g, Carex baccans Nees 12g, Stachys geobombycis C. Y. Wu 5g, plumage arrow 15g, Folium Cunninghamiae lanceolatae 12g, Radix rubi pungentis 15g, Salix wallichiana 15g, Creeper root 15g, Herba Bulbophylli inconspicui 12g, HUOMAYE 1g, Gorgon fruit 20g.

The preparation method of embodiment 1 is: Gryllotalpa, Creeper root, smelly Herba Artemisiae, Carex baccans Nees, Stachys geobombycis C. Y. Wu, plumage arrow, Herba Bulbophylli inconspicui, Salix wallichiana, HUOMAYE, Folium Cunninghamiae lanceolatae, Radix rubi pungentis, Gorgon fruit mixing and water adding are decocted secondary, each 1 hour, extracting solution filters, merge, simmer down to 80ml, be divided into twice, Neulized inhalation uses sooner or later.

The finished product that this kind of preparation method is prepared is liquid, and inconvenience stores, and also described filtered solution can be condensed into thick paste, drying under reduced pressure, pulverize 150 mesh sieves, becomes fine powder.During use, get 10g gained fine powder and add 50 DEG C of normal saline or water purification 40ml shakes up, carry out atomizing face mask suction.Every day 2 times.The extraction process preparing scheme Raw medicine due to above-mentioned two kinds is identical, is therefore only the change in finished product physical behavior, and pharmacology, drug effect are identical.

Embodiment 2, crude drug weight proportion: Gryllotalpa 1 part, smelly Herba Artemisiae 3 parts, Carex baccans Nees 3 parts, Stachys geobombycis C. Y. Wu 2 parts, 12 parts, plumage arrow, Folium Cunninghamiae lanceolatae 10 parts, Radix rubi pungentis 12 parts, Salix wallichiana 12 parts, Creeper root 12 parts, Herba Bulbophylli inconspicui 3 parts, HUOMAYE 1 part, 10 parts, Gorgon fruit.

Embodiment 3, crude drug weight proportion: Gryllotalpa 2 parts, smelly Herba Artemisiae 12 parts, Carex baccans Nees 12 parts, Stachys geobombycis C. Y. Wu 5 parts, 15 parts, plumage arrow, Folium Cunninghamiae lanceolatae 12 parts, Radix rubi pungentis 15 parts, Salix wallichiana 15 parts, Creeper root 15 parts, Herba Bulbophylli inconspicui 12 parts, HUOMAYE 1 part, 20 parts, Gorgon fruit

Embodiment 4, crude drug weight proportion: Gryllotalpa 3 parts, smelly Herba Artemisiae 12 parts, Carex baccans Nees 12 parts, Stachys geobombycis C. Y. Wu 2 parts, 20 parts, plumage arrow, Folium Cunninghamiae lanceolatae 15 parts, Radix rubi pungentis 20 parts, Salix wallichiana 20 parts, Creeper root 20 parts, Herba Bulbophylli inconspicui 12 parts, HUOMAYE 2 parts, 25 parts, Gorgon fruit.

The Chinese medicine of embodiment 2 ~ 4 can be prepared from by following method:

(1) by Folium Cunninghamiae lanceolatae, HUOMAYE, smelly Herba Artemisiae, Herba Bulbophylli inconspicui with vapor distillation extract volatile oil for subsequent use, medicinal residues are continued to employ;

(2) mixed with the medicinal residues of step 1 gained in Gryllotalpa, Creeper root, Radix rubi pungentis, Carex baccans Nees, Stachys geobombycis C. Y. Wu, plumage arrow, Salix wallichiana, Gorgon fruit and add 8 times amount soak by water twice, each 1 hour, extracting solution filters, and merges;

(3) the extracting solution concentrating under reduced pressure of step 2 gained is concentrated solution, and under concentrated solution 25 DEG C of conditions, relative density is 1.16; Under agitation, in concentrated solution, add 95% ethanol, make ethanol content to 70%, under 0 ~ 4 DEG C of cold preservation, leave standstill 24 hours, get supernatant liquid filtering, filtrate recycling ethanol, and be concentrated into every 1L and be equivalent to 5kg medical material;

(4) step 3 gained concentrated filtrate by 0.5% weight add powdered activated carbon, boil 30 minutes after stirring evenly, when letting cool to 50 DEG C, with 0.4um filter membrane sucking filtration;

(5) step 4 gained sucking filtration liquid by 0.2% weight add potassium sorbate mix homogeneously after, spray into the volatile oil of step 1 gained, mixing, subpackage.

During use, get 20ml Neulized inhalation liquid, add 50 DEG C of normal saline or water purification 20ml shakes up, carry out atomizing face mask suction.Every day 2 times.

The efficient combination of said medicine, coordinates mutually, effectively increase endoscopic stone extraction technique success rate, and side effect is less.The above results is clinical data sufficient proof, and interrelated data is as follows.

1 object and method.

1.1 object.

1.1.1 case selection: in January, 2004 ~ 2014 are diagnosed as " broncholithiasis " (intracavity type) and feasible patient 34 example that there is no atomization inspiration treatment contraindication of choosing date for operation through rabat, spiral CT and bronchoscopy year March.35 ~ 68 years old age, 54 years old mean age.

1.1.2 divide into groups: patient is divided into matched group 11 example, decoction atomization group 12 example and atomized liquid group 11 example.Compare in sex, age, the state of an illness, the course of disease before three groups of patient treatments, no significant difference (P>0.05), has comparability.

1.2 method.

1.2.1 Therapeutic Method: have the patient of respiratory symptom to take symptomatic treatment, comprises unobstructed, the suitable oxygen therapy in Hold up voltage road and infection control.Matched group is with normal saline 40ml Neulized inhalation; Decoction atomization group is with gained medicinal liquid 40ml Neulized inhalation in the embodiment of the present invention 1; Atomized liquid group, with gained Neulized inhalation liquid 20ml in the embodiment of the present invention 3, adds 50 DEG C of normal saline or water purification 20ml shakes up, and carries out atomizing face mask suction, every day 2 times.All patient treatments are after 2 weeks, lithotomy under row tracheascope.

1.2.2 statistical analysis: SPSS 16.0 carries out statistical analysis.Measurement data t checks, enumeration data χ ²inspection.

2 results.

2.1 3 groups of corrective surgery situations: under having 7 routine tracheascopies in decoction atomization group in 12 examples, lithotomy takes out calculus (58.33%), 5 customary open chest surgeries, and wherein focus is positioned at sub-segmental bronchus, tracheascope and foreign body forceps cannot arrive 2 examples; Under having 7 routine tracheascopies in atomized liquid group 11 example, lithotomy takes out calculus (63.64%), 4 customary open chest surgeries, and wherein focus is positioned at sub-segmental bronchus, and tracheascope and foreign body forceps cannot arrive 1 example; Under having 2 routine tracheascopies in matched group 11 example, lithotomy takes out calculus (18.18%), 9 customary open chest surgeries, and wherein focus is positioned at sub-segmental bronchus, and tracheascope and foreign body forceps cannot arrive 2 examples.

Stone success rate is got apparently higher than matched group (χ under decoction atomization group and the tracheascope of atomized liquid group ²=3.88, P<0.05; χ ²=4.70, P<0.05).

2.2 3 groups of adverse reactions of patients incidence rates compare: in treatment, obviously relevant to medicine untoward reaction does not all appear in three groups of patients.

3. conclusion.

This result of study shows, under tracheascope, before lithotomy, use the present invention to carry out decoction atomization group and the atomized liquid group of preoperative intervention, get stone success rate under tracheascope all apparently higher than the matched group of simple antiinflammatory, illustrate that pharmaceutical composition prepared by the inventive method has effective, that safety is higher advantage in broncholithiasis treatment.

Claims (1)

1. a preparation method for preoperative intervention atomized liquid, is characterized in that, be made up of following steps:

(1) by Folium Cunninghamiae lanceolatae, HUOMAYE, smelly Herba Artemisiae, Herba Bulbophylli inconspicui extract volatile oil for subsequent use, medicinal residues are continued to employ;

(2) the medicinal residues mixing and water adding of Gryllotalpa, Creeper root, Radix rubi pungentis, Carex baccans Nees, Stachys geobombycis C. Y. Wu, plumage arrow, Salix wallichiana, Gorgon fruit and step 1 gained is decocted twice, each 1 hour, extracting solution filters, and merges;

(3) the extracting solution concentrating under reduced pressure of step 2 gained; Under agitation, in concentrated solution, add ethanol, under 0 ~ 4 DEG C of cold preservation, leave standstill 24 hours, get supernatant liquid filtering, filtrate recycling ethanol, and concentrated;

(4) step 3 gained concentrated filtrate by 0.5% weight add powdered activated carbon, boil 30 minutes after stirring evenly, when letting cool to 50 DEG C, with 0.4um filter membrane sucking filtration;

(5) step 4 gained sucking filtration liquid by 0.2% weight add potassium sorbate mix homogeneously after, spray into the volatile oil of step 1 gained, mixing, subpackage.