CN104705259A - Dark moth wing gene utilization method in silkworm genetic breeding - Google Patents
Dark moth wing gene utilization method in silkworm genetic breeding Download PDFInfo
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- CN104705259A CN104705259A CN201510044662.3A CN201510044662A CN104705259A CN 104705259 A CN104705259 A CN 104705259A CN 201510044662 A CN201510044662 A CN 201510044662A CN 104705259 A CN104705259 A CN 104705259A
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 12
- 238000012214 genetic breeding Methods 0.000 title claims abstract description 9
- 108700003861 Dominant Genes Proteins 0.000 claims abstract description 39
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims description 21
- 230000009466 transformation Effects 0.000 claims description 16
- 238000007796 conventional method Methods 0.000 claims description 7
- 235000013601 eggs Nutrition 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 108700005079 Recessive Genes Proteins 0.000 claims description 5
- 102000052708 Recessive Genes Human genes 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 239000000975 dye Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 108091006146 Channels Proteins 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a dark moth wing gene utilization method in silkworm genetic breeding. Through a homozygosis method of rapidly introducing a dominant gene, the dominant gene is introduced into one breed of hybridized combination, so that on the basis that the degree of functionization of economic characters is not lowered, and by utilizing the differences of obvious moth wing body color between the dominant gene and another pair of hybridized breed of the hybridized combination, the purposes that pure pairs are distinguished and removed so that the hybrid rate of first generation hybrid cultivar can be improved are achieved. According to the homozygosis method of rapidly introducing the dominant gene, by utilizing the inherent characteristics of a homozygous individual of the dominant gene, individual transgenerational of the dominant gene is selected, the complete homozygous process of the dominant gene can be accelerated, and compared with a traditional breeding method, the number of selecting generations can be substantially reduced. According to the method, the number of generations needed by the gene homozygosis is small, the working procedure is simple, the cost is low, no pollution occurs, the economic characters are good, the practical value is high, by utilizing the preparation characteristics of the dark moth wing gene of the hybridized combination, the hybrid rate of the first generation hybrid cultivar can be substantially improved, and the occurrence that the hybrid rate indicator is not qualified is avoided.
Description
Technical field
The present invention relates to the breeding method of silkworm, refer to especially dark moth wing base in silkworm genetic breeding because of Application way.
Background technology
According to the regulation of national silkworm first generation cross-fertilize seed inspection procedure standard (NY T 327-1997), the hybrid rate of first generation cross-fertilize seed must not lower than 95%, traditional male and female pupa authentication technique is not only time-consuming, and often directly cause because male and female do not occur pure to mating respectively thoroughly and in a large number because technical measures are not in place, hybrid rate index is difficult to up to standard, causes silkworm egg by the gross to be scrapped.
In prior art, silkworm breeding has 2 kinds to avoid the pure technical method to occurring on producing:
1, some silkworm egg production units utilize dyestuff by the kind silkworm moth dyeing of in Hybrid, reach and distinguish pure right object, but this kind of method not only can increase recruitment in kind of field production of hybrid seeds process and input cost, and owing to employing industrial dye, cause silkworm moth can not continue to be applied to deep processing (as male bombycic wine etc.), produce new environmental pollution, do not meet the requirement of current " resources conservation, environmental friendliness ";
2, other silkworm egg production units utilize the silkworm moth of the darkening gene (mln/mln) that isozygotys to be grey black, this recessive gene is imported to a kind in hybrid combination in silkworm breeding process, this kind silkworm moth body colour is caused to be grey black, obvious with ordinary white silkworm moth difference, be easy to distinguish produce in first generation cross-fertilize seed manufacture process pure right, but due to the cocoon weight of this basic silkworm race, cocoon shell weight, Cocoon layer ratio is all lower, economic characters are poor, vitality is also lower, without practical value, need constantly transformation, particularly due to gene linkage, be difficult to increase substantially its practicality by Chromosome recombination technology, constrain current the applying of this veriety.
Summary of the invention
For prior art Problems existing, the object of the present invention is to provide and a kind ofly solve artificially colored technology and cause polluting and the method for the not high technical barrier of darkening genetic resources degree of being practical, use the dark moth wing genetic resources that the method will find in practical kind, the method of isozygotying is imported fast by its channel genes to the kind of in hybrid combination by a kind of dominant gene, make it on the degree of being practical basis not reducing economic characters, to utilize again in itself and hybrid combination another to hand over significant moth wing body colour difference between kind to reach to distinguish and reject pure right, thus improve the object of first generation cross-fertilize seed hybrid rate.Simultaneously, the feature of larger difference is had based on shade between the homozygous individual of this dominant gene and heterozygous individual, the object of isozygotying completely by selecting the darkest individual subculture of color can reach dominant gene fast in Breeding Process, to isozygoty required algebraically relative to the dominant gene in traditional breeding way, the method significantly can reduce the selection subculture number that this dominant gene isozygotys, and isozygoty thoroughly, exist without recessive gene c.
In silkworm genetic breeding provided by the invention dark moth wing base because of Application way, comprise the steps:
(1) cultivated silkworm breed variety that need transform is chosen as parent;
(2) with containing dark moth wing base because of basic silkworm race and the selected cultivated silkworm breed variety transformed that needs hybridize, obtain new proterties after through repeatedly backcrossing, how for selection cross excellent and there is the kind of dark moth wing feature:
(3) by the characteristic new varieties that step (2) obtains, another kind being ordinary white with moth wing combines new first generation cross-fertilize seed and combines, moth wing body colour between two kinds is utilized in this hybrid combination to there is the feature of significant difference, namely a kind is dark moth wing, another is ordinary white moth wing, distinguish and reject in first generation cross-fertilize seed manufacture process issuable pure right, thus reaching the object improving f_1 hybrid eggs of silkworm hybrid rate.
As preferably, the dark moth wing base imported in conventional variety in described step (2) is because dominant gene, and the color of dominant gene homozygous individual is the darkest, the color of the non-homozygous individual of dominant gene (heterozygous individual) is shallower than the color of its homozygous individual, based on this otherness feature, the algebraically that dominant gene can be made to reach object of isozygotying foreshortens to F
3generation, i.e. F
2the dominant gene homozygous individual subculture that generation selects moth wing color the darkest, F
3in generation, just can reach the dominant object of dark moth wing base because isozygotying completely, and the seed selection algebraically of traditional breeding method then at least more than 8 generations comes by generation superseded recessive individual, could realize the object of substantially isozygotying (as shown in Figure 1) of dominant gene:
Dominant dark moth wing gene C is imported in transformation material cc to selected need;
Be all dominant gene heterozygous individual, need transformation material cc selected by backcrossing, 50%;
Transformation material cc is needed, 75% selected by selection Cc individuality backcrosses;
Transformation material cc is needed, 87.5% selected by selection Cc individuality backcrosses;
Transformation material cc is needed, 93.75% selected by selection Cc individuality backcrosses;
Select the individual orthogonal of Cc, preparation F
2, the theoretical proportions 1-(1/2) of contained blood relationship composition
n+1;
The individual orthogonal subculture of the CC selecting color the darkest, and conventional method is superseded cc individuality;
Wherein CC individuality is 100%, and conventional method continues to eliminate cc individuality in CC, Cc, cc colony, until in the new seed selection material of F8 containing the ratio of dominant gene C more than 99%, but recessive gene c still has minor proportion to exist (dark moth wing base because representing with C, for dominant; Ordinary white moth wing c represents, is recessiveness; CC represents that dark moth wing base is because of homozygous individual, and color is the darkest; Cc represents that dark moth wing base is because of heterozygous individual, and color is more shallow; Cc represents that ordinary white is individual; F
1~ BC
3after percentage refer to that subculture individuality needs to transform the theoretical proportions of material blood relationship composition selected by containing).
The present invention is at F
2for time select the darkest individual subculture of moth wing color its gene pure algebraically can be foreshortened to F
3generation, relative to the basic step that dominant gene in silkworm genetic breeding conventional method isozygotys, is the fast method that a kind of special dominant gene isozygotys completely.
The beneficial effect that the present invention compared with prior art has is: gene pure algebraically of the present invention is few, operation is simple, cost is low, and without any pollution, economic characters are good, practical value is high, and utilize dark moth wing base in hybridization Combination nova because of preparation feature, namely kind be dark colored wing, and another is ordinary white, significantly can improve the hybrid rate of first generation cross-fertilize seed, avoid the phenomenon that silkworm egg is eliminated by the gross because hybrid rate index is defective to occur.
Accompanying drawing explanation
Fig. 1 is hybridization flow chart of the present invention.
Embodiment
Be illustrated in figure 1 the invention provides dark moth wing base in silkworm genetic breeding because of Application way, comprise the steps:
(1) cultivated silkworm breed variety that need transform is chosen as parent;
(2) with containing dark moth wing base because of basic silkworm race and the selected cultivated silkworm breed variety transformed that needs hybridize, obtain new proterties after through repeatedly backcrossing, how for selection cross excellent and there is the kind of dark moth wing feature:
Dominant dark moth wing gene C is imported in transformation material cc to selected need;
Be all dominant gene heterozygous individual, need transformation material cc selected by backcrossing, 50%;
Transformation material cc is needed, 75% selected by selection Cc individuality backcrosses;
Transformation material cc is needed, 87.5% selected by selection Cc individuality backcrosses;
Transformation material cc is needed, 93.75% selected by selection Cc individuality backcrosses;
Select the individual orthogonal of Cc, preparation F
2, the theoretical proportions 1-(1/2) of contained blood relationship composition
n+1;
The individual orthogonal subculture of the CC selecting color the darkest, and conventional method is superseded cc individuality;
Wherein CC individuality is 100%, and conventional method continues in CC, Cc, cc colony, eliminate cc individuality, until F
8ratio containing dominant gene C in new seed selection material is more than 99%, but recessive gene c still has minor proportion to exist;
Wherein, dark moth wing base because representing with C, for dominant; Ordinary white moth wing c represents, is recessiveness; CC represents that dark moth wing base is because of homozygous individual, and color is the darkest; Cc represents that dark moth wing base is because of heterozygous individual, and color is more shallow; Cc represents that ordinary white is individual; F
1~ BC
3after percentage refer to that subculture individuality needs to transform the theoretical proportions of material blood relationship composition selected by containing;
(3) by the characteristic new varieties that step (2) obtains, another kind being ordinary white with moth wing combines new first generation cross-fertilize seed and combines, moth wing body colour between two kinds is utilized in this combination to there is the feature of significant difference, namely a kind is dark moth wing, another is ordinary white, distinguish and reject in first generation cross-fertilize seed manufacture process issuable pure right, thus reaching the object improving f_1 hybrid eggs of silkworm hybrid rate.
Dark moth wing base is because dominant gene, and the moth wing color of dominant gene homozygous individual is the darkest, and the color of the non-homozygous individual of dominant gene (i.e. dominant gene heterozygous individual) is shallower than its homozygous individual.In step (2) process, based on this otherness feature, the algebraically in traditional breeding way at least more than 8 generations can be come eliminate recessive individual by generation, reach the required algebraically that dominant gene isozygotys and foreshorten to F
3generation (F
1generation is all dominant gene heterozygous individual, F
2occur in colony that partial dominance gene pure is individual).I.e. F
2the dominant gene homozygous individual subculture that generation selects moth wing color the darkest, F
3in generation, just can reach the dominant object of dark moth wing base because isozygotying completely.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. in silkworm genetic breeding dark moth wing base because of Application way, it is characterized in that, comprise the steps:
(1) cultivated silkworm breed variety that need transform is chosen as parent;
(2) with containing dark moth wing base because of basic silkworm race and the selected cultivated silkworm breed variety transformed that needs hybridize, obtain new proterties after through repeatedly backcrossing, how for selection cross excellent and there is the kind of dark moth wing feature:
(3) by the characteristic new varieties that step (2) obtains, another kind being ordinary white with moth wing combines new first generation cross-fertilize seed and combines, moth wing body colour between two kinds is utilized in this hybrid combination to there is the feature of significant difference, namely a kind is dark moth wing, another is ordinary white moth wing, distinguish and reject in first generation cross-fertilize seed manufacture process issuable pure right, thus reaching the object improving f_1 hybrid eggs of silkworm hybrid rate.
2. in silkworm genetic breeding according to claim 1 dark moth wing base because of Application way, it is characterized in that: the dark moth wing base imported in conventional variety in described step (2) is because dominant gene, and the color of dominant gene homozygous individual is the darkest, the color of the non-homozygous individual of dominant gene (heterozygous individual) is shallower than the color of its homozygous individual, based on this otherness feature, the algebraically that dominant gene can be made to reach object of isozygotying foreshortens to F
3generation, i.e. F
2in generation, selects the darkest dominant gene homozygous individual of moth wing color to carry out subculture, F
3in generation, just can reach the dominant object of dark moth wing base because isozygotying completely, and the seed selection algebraically of traditional breeding method then at least more than 8 generations comes by generation superseded recessive individual, could realize the object of substantially isozygotying (as shown in Figure 1) of dominant gene:
Dominant dark moth wing gene C is imported in transformation material cc to selected need;
Be all dominant gene heterozygous individual, need transformation material cc selected by backcrossing, 50%;
Transformation material cc is needed, 75% selected by selection Cc individuality backcrosses;
Transformation material cc is needed, 87.5% selected by selection Cc individuality backcrosses;
Transformation material cc is needed, 93.75% selected by selection Cc individuality backcrosses;
Select the individual orthogonal of Cc, preparation F
2, the theoretical proportions 1-(1/2) of contained blood relationship composition
n+1;
The individual orthogonal subculture of the CC selecting color the darkest, and conventional method is superseded cc individuality;
Wherein CC individuality is 100%, and conventional method continues in CC, Cc, cc colony, eliminate cc individuality, until F
8in new seed selection material containing the ratio of dominant gene C more than 99%, but recessive gene c still has minor proportion to exist (dark moth wing base because representing with C, for dominant; Ordinary white moth wing c represents, is recessiveness; CC represents that dark moth wing base is because of homozygous individual, and color is the darkest; Cc represents that dark moth wing base is because of heterozygous individual, and color is more shallow; Cc represents that ordinary white is individual; F
1~ BC
3after percentage refer to that subculture individuality needs to transform the theoretical proportions of material blood relationship composition selected by containing).
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| CN104705259A true CN104705259A (en) | 2015-06-17 |
| CN104705259B CN104705259B (en) | 2018-03-27 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU793540A1 (en) * | 1979-07-24 | 1981-01-07 | Главное Управление Шелководствауправления Сельского Хозяйстваузбекской Ccp | Device for obtaining silkworm seed |
| CN1194093A (en) * | 1997-03-21 | 1998-09-30 | 胡祥平 | Technology for artificially cultivating china wood frog |
| CN1296741A (en) * | 2000-11-09 | 2001-05-30 | 上海丰康科技开发有限公司 | Technique for reproducing and raising Chinese wood frog in souch of China |
| RO125576A0 (en) * | 2009-11-25 | 2010-07-30 | Universitatea De Ştiinţe Agricole Şi Medicina Veterinară Cluj-Napoca | Methods for maintaining the breeds in the genetic fund of bombyx mori l species |
| CN201640211U (en) * | 2010-04-20 | 2010-11-24 | 张凤春 | Tadpole hatching breeding pool |
| CN102972360A (en) * | 2012-12-28 | 2013-03-20 | 湖南省蚕桑科学研究所 | Method for applying silkworm moth dark flowery wing gene to domestic silkworm genetic breeding |
-
2015
- 2015-01-25 CN CN201510044662.3A patent/CN104705259B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU793540A1 (en) * | 1979-07-24 | 1981-01-07 | Главное Управление Шелководствауправления Сельского Хозяйстваузбекской Ccp | Device for obtaining silkworm seed |
| CN1194093A (en) * | 1997-03-21 | 1998-09-30 | 胡祥平 | Technology for artificially cultivating china wood frog |
| CN1296741A (en) * | 2000-11-09 | 2001-05-30 | 上海丰康科技开发有限公司 | Technique for reproducing and raising Chinese wood frog in souch of China |
| RO125576A0 (en) * | 2009-11-25 | 2010-07-30 | Universitatea De Ştiinţe Agricole Şi Medicina Veterinară Cluj-Napoca | Methods for maintaining the breeds in the genetic fund of bombyx mori l species |
| CN201640211U (en) * | 2010-04-20 | 2010-11-24 | 张凤春 | Tadpole hatching breeding pool |
| CN102972360A (en) * | 2012-12-28 | 2013-03-20 | 湖南省蚕桑科学研究所 | Method for applying silkworm moth dark flowery wing gene to domestic silkworm genetic breeding |
Non-Patent Citations (4)
| Title |
|---|
| 杜晓华等: "《园林植物遗传育种学》", 30 April 2013, 中国水利水电出版社 * |
| 杨明观等: "桑蚕暗花蛾突变体及其在生产上的应用前景", 《蚕桑通报》 * |
| 郭玉华: "《遗传学》", 30 June 2014, 中国农业大学出版社 * |
| 长岛荣一: "家蚕育种的理论与方法", 《蚕学通讯》 * |
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