CN107667976A - A kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing - Google Patents
A kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing Download PDFInfo
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- CN107667976A CN107667976A CN201711085475.5A CN201711085475A CN107667976A CN 107667976 A CN107667976 A CN 107667976A CN 201711085475 A CN201711085475 A CN 201711085475A CN 107667976 A CN107667976 A CN 107667976A
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- 235000014676 Phragmites communis Nutrition 0.000 title claims abstract description 76
- 210000003746 feather Anatomy 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 21
- 241000287828 Gallus gallus Species 0.000 claims abstract description 40
- 230000008774 maternal effect Effects 0.000 claims abstract description 31
- 230000008775 paternal effect Effects 0.000 claims abstract description 30
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 25
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 238000009396 hybridization Methods 0.000 claims abstract description 13
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 10
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 10
- 210000003278 egg shell Anatomy 0.000 claims abstract description 10
- RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 claims abstract description 4
- 238000004519 manufacturing process Methods 0.000 claims description 18
- 238000002474 experimental method Methods 0.000 claims description 2
- 238000011017 operating method Methods 0.000 claims description 2
- 239000003550 marker Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000012214 genetic breeding Methods 0.000 abstract description 2
- 244000144977 poultry Species 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 description 33
- ONTQJDKFANPPKK-UHFFFAOYSA-L chembl3185981 Chemical compound [Na+].[Na+].CC1=CC(C)=C(S([O-])(=O)=O)C=C1N=NC1=CC(S([O-])(=O)=O)=C(C=CC=C2)C2=C1O ONTQJDKFANPPKK-UHFFFAOYSA-L 0.000 description 4
- 230000036750 egg laying performance Effects 0.000 description 3
- 230000009027 insemination Effects 0.000 description 3
- 210000003765 sex chromosome Anatomy 0.000 description 3
- 210000000436 anus Anatomy 0.000 description 2
- 210000000991 chicken egg Anatomy 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 108091006146 Channels Proteins 0.000 description 1
- 108700003861 Dominant Genes Proteins 0.000 description 1
- 208000021018 autosomal dominant inheritance Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Animal Husbandry (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract
The invention belongs to poultry Biotechnology in Genetic Breeding field, the producing method for seed of the high yield type reed catkins feather layer of green-shell egg of more particularly to a kind of automatic sexing.The producing method for seed of the high yield type reed catkins feather layer of green-shell egg of the automatic sexing, using non-reed catkins plumage high-yield egg chicken A systems and non-reed catkins plumage high-yield egg chicken B systems, form the first paternal A and the first maternal B；It is the second maternal AB to be stayed after the maternal B hybridization pure breedings of first paternal A and first and select high yield, slow plumage, non-reed catkins plumage, non-green shell hen；Fast plumage, non-green shell cock are selected in reed catkins chicken strain as male parent, fast plumage, green shell hen are selected in non-reed catkins chicken strain as maternal, pure breeding after both hybridize, selects fast plumage, reed catkins plumage, oocyan gene homozygous, is terminal paternal line C；The maternal AB of terminal paternal line C and second hybridize to obtain commercial generation, and slow plumage is cock, and fast plumage is hen.The high yield type reed catkins feather layer of green-shell egg of the automatic sexing obtained using the producing method for seed has the advantages of high yield, Down co1or sexing, eggshell are green shell.
Description
Technical field
The invention belongs to poultry Biotechnology in Genetic Breeding field, the green shell of high yield type reed catkins plumage of more particularly to a kind of automatic sexing
The producing method for seed of laying hen.
Background technology
China is with a vast territory, and Germplasm Resources of Local enriches, and different geographical forms the kind with local characteristic, hallmark
Shape protrudes.Wenshang reed catkins chicken has the nutritious advantage of good appearance, delicious meat, egg product, but its yield is relatively low.Green shell chicken
Egg bright color, is loved by consumers, therefore market price is higher, but there is also egg production it is low the shortcomings that.Green-egg-shelled this
Qualitative character is dominant gene control on autosome, therefore if green-egg-shelled channel genes Wenshang reed catkins chicken, cultivation has
The green shell new lines of Wenshang reed catkins chicken appearance traits feature, then itself and high-yield variety are hybridized, improve commercial generation layer of green-shell egg
Egg laying performance will be significant.Automatic sexing system is established using the sex-linked inheritance principle of Feathering type, realizes reed catkins plumage
The polymerization of shape, green-egg-shelled character, high-yield character and speed feather genes, cultivate have keep Wenshang reed catkins chicken appearance traits feature,
Produce green-egg-shelled, the new varieties that egg laying performance greatly improves, by with wide market prospects.
The content of the invention
The present invention is in order to make up the deficiencies in the prior art, there is provided a kind of seed selection science, reasonable and simple to operate from not
The producing method for seed of the high yield type reed catkins feather layer of green-shell egg of male and female, the high yield type reed catkins of the automatic sexing obtained using the producing method for seed
Feather layer of green-shell egg has the advantages of high yield, Down co1or sexing, eggshell are green shell, solves problems of the prior art.
The present invention is achieved through the following technical solutions：A kind of system of the high yield type reed catkins feather layer of green-shell egg of automatic sexing
Kind method, including following operating procedure：
S1：It is tethered at using non-reed catkins plumage high-yield egg chicken A and selects slow plumage, non-green shell individual, forms the first paternal A；Utilize non-reed
Flower plumage high-yield egg chicken B systems, stay and select slow plumage, non-green shell individual, form the first maternal B；
S2：First paternal A and the first maternal B hybridization are obtained to stay after F1 pure breedings and select high yield, slow plumage, non-reed catkins plumage, non-green
Shell hen is the second maternal AB；
S3：Fast plumage, non-green shell cock are selected in reed catkins chicken strain as male parent, is selected in non-reed catkins chicken strain fast
As maternal, pure breeding after both are hybridized, selection target is fast plumage, reed catkins plumage, green shell homozygous gene, and energy for plumage, green shell hen
The strain of enough stable heredity is terminal paternal line C；
S4：By the maternal AB of terminal paternal line C and second hybridization obtain can automatic sexing, there is high yield, reed catkins plumage, green shell
The commercial generation of character, wherein slow plumage is cock, fast plumage is hen.
The fast feather genes of the male parent, oocyan gene and reed catkins feather genes are respectively homozygous genotype Z in step s3kZk、
OO and ZBZB。
In step s3, by the F1' obtained after male parent and hybridization of female parent generations, reed catkins plumage, fast plumage, while hen are selected
The individual pure breeding of green-egg-shelled is produced, is selected and remain in obtained F2' generations according to same selection standard and continues multiple pure breeding, carried out simultaneously
Molecular marker assisted selection and test cross experiment, the fast plumage, reed catkins plumage, green shell strain for finally cultivating into homozygous genotype are terminal father
It is C.
In step s 4, terminal paternal line C eggshell color character gene type is dominant homogeneous genotype OO, by its with it is non-green
The oo hybridization of shell genotype, offspring's genotype of acquisition is Oo, and the phenotype of eggshell color is green.
In step s 4, terminal paternal line C reed catkins plumage character gene is dominant homogeneous genotype ZBZB, by itself and genotype
For ZbAfter second maternal AB hybridization of W non-reed catkins plumage, the plumage color of commercial generation cock and hen shows as reed catkins plumage color.
The present invention has the advantages that：Preparation method of the present invention is simple, using three series mating producing method for seed, with high yield
Slow plumage strain is the first paternal A, using the slow plumage strain of high yield as the first maternal B, the maternal B of the first paternal A and first is hybridized, obtained
Second maternal AB of the slow plumage of high yield, using the strain of homozygous plumage, reed catkins, green shell soon as terminal paternal line C, by the second maternal AB and terminal
Paternal C assemblings production breed system, has following technical advantage：
1st, the second maternal AB high egg productions, egg size is suitable, and food utilization efficiency is high, and often covering the week old of Parent stock 72 can produce
Commercial generation hen 110, reduces breeding cost；
2nd, for the egg production of the week old of Wenshang reed catkins chicken 72 typically within 180, commercial generation egg production of the invention is more purebred
Wenshang reed catkins chicken improves about 45%, and the commercial generation high egg production of 72 week old is up to more than 260；
3rd, the eggshell color of commercial generation chicken is green, meets consumption demand of the consumer to eggshell color；
4th, the present invention produces the appearance traits feature that commercial generation male and female chicken maintains Wenshang reed catkins chicken, deep joyous by consumer
Meet；
5th, the commercial generation of final production can avoid to turn over caused by anus differentiates possibility and intersect dirt according to plumage speed automatic sexing
Dye, the cost differentiated is saved.
The invention adapts to consumer need of the consumer to characteristic soil species chicken, the commercial generation cock produced and the value of hen
More common laying hen significantly improves, and realizes the creative utilization to China's improved seeds resource, has good economic and social benefits.
Brief description of the drawings
The present invention is further illustrated below in conjunction with the accompanying drawings.
Fig. 1 is the breed system production of hybrid seeds ideograph of the high yield type reed catkins feather layer of green-shell egg of the automatic sexing of the present invention；
Fig. 2 is the breed system production of hybrid seeds gene genetic of the high yield type reed catkins feather layer of green-shell egg of the automatic sexing of the embodiment of the present invention
Figure；
Fig. 3 is the green shell of the present invention, reed catkins plumage homozygosis terminal paternal line C seed selection flow charts；
Wherein：
Z/W：The sex chromosome of chicken is expressed as, ZZ is male, and ZW is female；
K/k：Slow plumage and fast plumage character gene are represented, is sex-linked inheritance gene on sex chromosome Z；
B/b：Reed catkins plumage and non-reed catkins feather genes are represented, are sex-linked inheritance gene on sex chromosome Z；
O/o：Green shell and non-oocyan gene are represented, is autosomal dominant inheritance gene on autosome.
Embodiment
For the technical characterstic for illustrating this programme can be understood, below by embodiment, and its accompanying drawing is combined, to this hair
It is bright to be described in detail, but the invention is not limited in this.
Embodiment
As Figure 1-3, the producing method for seed of the high yield type reed catkins feather layer of green-shell egg of the automatic sexing, including following operation step
Suddenly：
S1：It it is great-grandfather's generation using the red No. 1 laying hen A system cocks in high-yield egg chicken capital and hen, 1 age in days, which stays, after hatching selects slow plumage
Individual is used for the first paternal A cultivation, and heterozygous genes, Select gene type ♂ Z are eliminated by means such as traversed by measureKbZKbOo, ♀
ZKbWoo carry out crossbreed, and eliminate weak young bird, using individual cage raise, artificial insemination, quantity 500 of each reserving seed for planting from generation to generation only with
On, male and female ratio 1:10；Finally stay and select homozygous genotype cock ZKbZKbOo, form the first paternal A；
It it is great-grandfather's generation using the red No. 1 laying hen B system cocks in high-yield egg chicken capital and hen, 1 age in days, which stays, after hatching selects slow plumage
Body is used for the first maternal B seed selection, eliminates heterozygous genes by means such as traversed by measure, Select gene type is ♂ ZKbZKbOo and ♀
ZKbWoo carry out crossbreed, and eliminate weak young bird, using individual cage raise, artificial insemination, quantity 2000 of each reserving seed for planting from generation to generation only with
On, male and female ratio 1:10；Finally stay and select homozygous genotype hen ZKbWoo, form the first maternal B.
S2：The maternal B hybridization of first paternal A and first is obtained into F1, you can genotype is obtained as the second homozygous maternal AB,
Genotype is ZKbWoo；
Distinguish during this production of hybrid seeds first with red No. 1 laying hen B systems of the red No. 1 laying hen A system in high-yield egg chicken capital and high-yield egg chicken capital
Breed and turn out the first paternal A and the first maternal B, recycle the first paternal A and first with highly yielding ability and character stabilization
Maternal B individuals hybridization, obtain slow plumage and with the second maternal AB for stablizing hereditary capacity, this lays eggs to the follow-up commercial generation that improves
Measurer plays an important role.
S3：Fast plumage, non-green shell cock are selected in the reed catkins chicken of Wenshang as male parent, its genotype is ♂ ZkBZkBOo, in east
Fast plumage, green shell hen are selected in township's layer of green-shell egg as female parent, its genotype is ♀ ZkbWOO, the F1' generations that both obtain after hybridizing
It is fast plumage, reed catkins plumage, green shell, the genotype of cock is ♂ ZkBZkbOo, the genotype of hen is ♀ ZkBWOo；Then carry out pure
It is numerous, retain fast plumage, reed catkins plumage, the individual of green shell character in obtained F2' generations, the cock genotype now obtained may be
ZkBZkBOo、ZkBZkBOO、ZkBZkbOo、ZkBZkbOO, hen genotype may be ZkBWOo、ZkBWOO, aid in selecting by molecular labeling
Select or the method for test cross, select homozygous genotype ♂ Z respectivelykBZkBOO、♀ZkBWOO individual carries out pure breeding again, and eliminates weak
Young bird, retain strong young bird, raised using single cage, artificial insemination, quantity of each reserving seed for planting from generation to generation more than 500, male and female ratio 1:10；Finally
It is terminal paternal line C to stay and select homozygous cock, and its genotype is ♂ ZkBZkBOO。
S4：By above-mentioned terminal paternal line C ♂ ZkBZkBThe maternal AB ♀ Z of OO and secondKbWoo, which hybridizes to obtain F2, obtains commercial generation,
Phenotype is high yield, reed catkins plumage, green shell character, and wherein cock is slow plumage, and its genotype is ♂ ZkBZKbOo, hen are fast plumage,
Its genotype is ♀ ZkBWOo, commercial generation chicken can be differentiated rapidly by Feathering type.
In the above method, the second maternal AB seed selection, which can also use, obtains the method that the blue A systems in sea and B systems are hybridized,
Seed selection and method for breeding are the same as described in step S1.
The cage pattern that the commercial generation of the present invention can produce according to modern laying hen, have feeding efficiency high, be easy to manage, have
The advantages of beneficial to Product quality and safety is ensured.
Experimental example
1st, Parent stock production performance contrasts, and is shown in Table 1：
Experimental group：The high yield type reed catkins feather layer of green-shell egg Parent stock of automatic sexing；Control group：Purebred Wenshang reed catkins father
Mother is for breeder.
The Parent stock production performance that the present invention of table 1 is cultivated
As can be seen from Table 1, the production performance of Parent stock of the invention is higher, than being mother with purebred Wenshang reed catkins chicken
This, 72 week old egg numbers improve more than 120, improve more than 60% than Wenshang reed catkins chicken egg productivity, breeding cost significantly reduces；
Father and mother godmother chicken is the paternal hen during the high-yield egg chicken production of hybrid seeds, and plumage color is recessive golden plumage, so the cock with reed catkins plumage
Its offspring's plumage color shows as reed catkins plumage after hybridization, and (week old of high-yield egg chicken 72 is laid eggs 300 left sides for its production performance and high-yield egg chicken
It is right) quite, it is seen that the Parent stock of this producing method for seed has higher egg laying performance.
2nd, commercial generation chicken synthesis production performance contrast, is shown in Table 2：
Experimental group：The high yield type reed catkins feather layer of green-shell egg commercial generation cock (abbreviation commercial generation cock) of automatic sexing, certainly not
The high yield type reed catkins feather layer of green-shell egg commercial generation hen (abbreviation commercial generation hen) of male and female；Control group：Purebred Wenshang reed catkins chicken business
Product are for cock, purebred Wenshang reed catkins chicken commercial generation hen.
The commercial generation chicken character pair ratio of table 2
Interpretation of result：As can be seen from Table 2,1, the eggshell color that the commercial generation that the present invention cultivates is laid eggs is green, full
Foot consumption demand of the consumer to eggshell color；2nd, the week old high egg production of commercial generation 72 of the invention is up to 260, more purebred river in Shangdong Province
Upper reed catkins chicken is improved more than 45%, enhances the market competitiveness；3rd, commercial generation cock of the present invention is slow plumage, and commercial generation hen is equal
For fast plumage, it can avoid according to plumage speed automatic sexing and turn over cross pollution caused by anus differentiates possibility, saved the cost differentiated.
Claims (5)
1. a kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing, it is characterized in that, including following operating procedure：
S1：It is tethered at using non-reed catkins plumage high-yield egg chicken A and selects slow plumage, non-green shell individual, forms the first paternal A；Utilize non-reed catkins plumage
High-yield egg chicken B systems, stay and select slow plumage, non-green shell individual, form the first maternal B；
S2：Select high yield, slow plumage, non-reed catkins plumage, non-green shell female by being stayed after the maternal B hybridization acquisition F1 pure breedings of the first paternal A and first
Chicken is the second maternal AB；
S3：Fast plumage, non-green shell cock are selected in reed catkins chicken strain as male parent, fast plumage, green is selected in non-reed catkins chicken strain
For shell hen as maternal, pure breeding after both are hybridized, selection target is fast plumage, reed catkins plumage, green shell homozygous gene, and can be stablized
The strain of heredity is terminal paternal line C；
S4：By the maternal AB of terminal paternal line C and second hybridization obtain can automatic sexing, there is high yield, reed catkins plumage, green shell character
Commercial generation, wherein slow plumage is cock, fast plumage is hen.
2. a kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing according to claim 1, its feature
It is：The fast feather genes of the male parent, oocyan gene and reed catkins feather genes are respectively homozygous genotype Z in step s3kZk, OO and
ZBZB。
3. a kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing according to claim 2, its feature
It is：In step s3, by the F1' obtained after male parent and hybridization of female parent generations, reed catkins plumage, fast plumage are selected, while hen production is green
The individual pure breeding of shell egg, selected and remain in obtained F2' generations according to same selection standard and continue multiple pure breeding, while carry out molecule
Marker assisted selection and test cross experiment, the fast plumage, reed catkins plumage, green shell strain for finally cultivating into homozygous genotype are terminal paternal line C.
4. a kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing according to claim 1, its feature
It is：In step s 4, terminal paternal line C eggshell color character gene type is dominant homogeneous genotype OO, by itself and non-green shell base
Because the oo of type hybridizes, offspring's genotype of acquisition is Oo, and the phenotype of eggshell color is green.
5. a kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing according to claim 1, its feature
It is：In step s 4, terminal paternal line C reed catkins plumage character gene is dominant homogeneous genotype ZBZB, it is Z by itself and genotypebW
Non- reed catkins plumage the second maternal AB hybridization after, the plumage color of commercial generation cock and hen shows as reed catkins plumage color.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108496896A (en) * | 2018-04-09 | 2018-09-07 | 徐杰 | A kind of green shell plumage color of high yield is from other laying hen breeding method and its application |
| CN109122579A (en) * | 2018-09-30 | 2019-01-04 | 辽宁农业职业技术学院 | A method of it improving large bone chicken Feathering type automatic sexing and identifies accuracy rate |
| CN109315350A (en) * | 2018-07-12 | 2019-02-12 | 湖北欣华生态畜禽开发有限公司 | A kind of breeding method of the green shell high-yield egg chicken of recessive white feather short-foot |
| CN111345267A (en) * | 2020-04-08 | 2020-06-30 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Breeding method of hybrid line of special laying hens with feather color double-self-distinguishing male and female black-feather pink shells and high yield |
| CN114667965A (en) * | 2022-02-17 | 2022-06-28 | 江苏省家禽科学研究所科技创新有限公司 | Method for breeding high-reproduction, high-efficiency and high-quality dual-purpose black-feather chickens |
| CN115226671A (en) * | 2022-08-05 | 2022-10-25 | 长顺天农绿壳蛋鸡实业有限公司 | Method for producing hybrid seeds of self-sexing Changshun green-shell laying hens matched line |
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| CN108496896A (en) * | 2018-04-09 | 2018-09-07 | 徐杰 | A kind of green shell plumage color of high yield is from other laying hen breeding method and its application |
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| CN111345267A (en) * | 2020-04-08 | 2020-06-30 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Breeding method of hybrid line of special laying hens with feather color double-self-distinguishing male and female black-feather pink shells and high yield |
| CN111345267B (en) * | 2020-04-08 | 2021-11-19 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Breeding method of hybrid line of special laying hens with feather color double-self-distinguishing male and female black-feather pink shells and high yield |
| CN114667965A (en) * | 2022-02-17 | 2022-06-28 | 江苏省家禽科学研究所科技创新有限公司 | Method for breeding high-reproduction, high-efficiency and high-quality dual-purpose black-feather chickens |
| CN115226671A (en) * | 2022-08-05 | 2022-10-25 | 长顺天农绿壳蛋鸡实业有限公司 | Method for producing hybrid seeds of self-sexing Changshun green-shell laying hens matched line |
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